MD06G1182900.v1.1

RING/Ubox like zinc-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0005751
Physical Location & Seq
Reverse (-)
32138329 .. 32140103
1775 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1182900.v1.1.491

Sequence Viewer

Length: 1023 bp
ATGCTTTCCGATGCAATTACCAGCGCCTCCGTCCATGCAGCCCCGAACAGCGGCGGCAGGGATTTGGGGAAGAAGAAGAGGGCCAGGTCTGCCAAATTGAAGCAGTGCAAGCTCGATGTTCGTCGGGAGCAATGGCTTTCTCAAGGCGCTGTGAAGAACAAGGATTGCAAGGAGGAACTGAACGGCGTCGTTAAAGCGGGAAAAGAGCGGAATCACAACCGTTCATTTGGTAATCTGGGGATGAGGCCCAGGGGTGGACAGATTGACGGATCGATCCACCATCACCATCACCATGACAGCGATTTGGAGTCGAACAGTCCGACCAGTTTGACAAGTAGTGTATTGGGCAGCAACTATTCCGAGACTACATTTACTAGCAGCAGTAGTAGCAGCGGTGGCTGTTGCTCTGGTAATATCACAGAGGATGAGGAAGGTGGTGGTGACGATGGTTGCTTGGACGATTGGGAAGCGGTGGCTGACGCCTTAGCTGCGGATGAGAAGCCGAAAATCCCATGTCCAGAATCGCCCCCAGAGCATGATCCAATTGCTCAAATGGTTTCTCCTCAGGAAACGACTATTGGGTCTGATGGGGTTGAGAATTTGAACCCTCAGTGCGGCAGAGCTATCCCTAAAGCTTGGGGGAATGGCCATGCTTGGAGGCCTGACGATGCTTTTCGGCCTCAGAGTCTGCCCAATTTGGCTAAGCAGCTTAGCTTGCCGAATTCAAACCGGAAACATTACAGTTGTGGTGGTGTTCCTTGGGCCTATAATGGTGGTGCCGTGCCATCCTCGTGTCCTATATGCTATGAAGATTTGGATTTCACGGACACGAGCTTTTTGCCTTGCTTGTGCGGATTCCGGCTCTGCCTTTTCTGCCACAATAGGATTGTGGAGGAGGATGGCCGCTGTCCCGGCTGCAGGAAGCCATACGAGCATGAGCCTGTTGAGGCAGGGACAAGTGTGCATGGAGGAAGTCTCACATTTCAGCTGCCTCGTTCTTGTAGCATGATCACAAGGTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000077 GO:0000278 GO:0000288 GO:0000289 GO:0000956 GO:0002682 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006139 GO:0006401 GO:0006402 GO:0006464 GO:0006508 GO:0006511 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006977 GO:0007049 GO:0007093 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009892 GO:0009987 GO:0010468 GO:0010564 GO:0010605 GO:0010629 GO:0010948 GO:0016070 GO:0016071 GO:0016567 GO:0016740 GO:0019222 GO:0019439 GO:0019538 GO:0019787 GO:0019941 GO:0022402 GO:0023052 GO:0030163 GO:0030330 GO:0031570 GO:0031571 GO:0032446 GO:0033554 GO:0034641 GO:0034655 GO:0035556 GO:0036211 GO:0042770 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0044773 GO:0044774 GO:0044783 GO:0044819 GO:0045595 GO:0045637 GO:0045652 GO:0045786 GO:0045787 GO:0045930 GO:0046483 GO:0046700 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051603 GO:0051716 GO:0051726 GO:0051865 GO:0060255 GO:0065007 GO:0070647 GO:0071156 GO:0071158 GO:0071704 GO:0072331 GO:0072395 GO:0072401 GO:0072413 GO:0072422 GO:0072431 GO:0090068 GO:0090304 GO:0140096 GO:1901360 GO:1901361 GO:1901564 GO:1901565 GO:1901575 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902400 GO:1902402 GO:1902403 GO:1902806 GO:1902807 GO:1903047 GO:1903706 GO:2000026 GO:2000045 GO:2000134
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_4

Protein Analysis

341

Amino Acids

36.66

Weight (kDa)

6.06

Isoelectric Point (pI)

54.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_4 PF14570 265 - 311 5.6e-18 RING/Ubox like zinc-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 580
AccB1I GGYRCC 1 cut(s) 776
AccBSI CCGCTC 1 cut(s) 208
AclWI GGATC 3 cut(s) 268, 277, 533
AcoI YGGCCR 2 cut(s) 646, 901
AcsI RAATTY 2 cut(s) 598, 721
AcyI GRCGYC 2 cut(s) 186, 480
AfiI CCNNNNNNNGG 4 cut(s) 50, 254, 614, 918
AgsI TTSAA 3 cut(s) 100, 604, 726
AjnI CCWGG 2 cut(s) 83, 248
AjuI GAANNNNNNNTTGG 2 cut(s) 561, 593
AluBI AGCT 8 cut(s) 112, 488, 623, 635, 709, 714, 834, 988
AluI AGCT 8 cut(s) 112, 488, 623, 635, 709, 714, 834, 988
Alw26I GTCTC 2 cut(s) 356, 980
AlwI GGATC 3 cut(s) 268, 277, 533
AlwNI CAGNNNCTG 1 cut(s) 688
AoxI GGCC 7 cut(s) 81, 245, 646, 659, 677, 762, 901
ApeKI GCWGC 8 cut(s) 38, 348, 378, 390, 488, 706, 915, 988
ApoI RAATTY 2 cut(s) 598, 721
AspLEI GCGC 2 cut(s) 26, 149
AspS9I GGNCC 3 cut(s) 81, 246, 762
AsuC2I CCSGG 1 cut(s) 912
AsuHPI GGTGA 3 cut(s) 275, 281, 452
AxyI CCTNAGG 1 cut(s) 564
BalI TGGCCA 1 cut(s) 648
BanI GGYRCC 1 cut(s) 776
BauI CACGAG 2 cut(s) 790, 829
BbvI GCAGC 8 cut(s) 50, 360, 390, 402, 475, 718, 902, 975
BccI CCATC 6 cut(s) 288, 294, 440, 581, 793, 893
BceAI ACGGC 2 cut(s) 199, 764
BcgI CGANNNNNNTGC 2 cut(s) 820, 854
BciT130I CCWGG 2 cut(s) 85, 250
BclI TGATCA 1 cut(s) 1008
BcnI CCSGG 1 cut(s) 912
BcoDI GTCTC 2 cut(s) 356, 980
BfaI CTAG 1 cut(s) 375
BfmI CTRYAG 1 cut(s) 916
BfoI RGCGCY 2 cut(s) 27, 150
BlpI GCTNAGC 2 cut(s) 702, 710
Bme1390I CCNGG 3 cut(s) 85, 250, 912
BmgT120I GGNCC 3 cut(s) 81, 246, 762
BmiI GGNNCC 1 cut(s) 778
BmrFI CCNGG 3 cut(s) 85, 250, 912
BmsI GCATC 1 cut(s) 658
BplI GAGNNNNNCTC 2 cut(s) 960, 992
Bpu10I CCTNAGC 1 cut(s) 484
Bpu1102I GCTNAGC 2 cut(s) 702, 710
BpuEI CTTGAG 1 cut(s) 126
BpuMI CCSGG 1 cut(s) 912
Bsa29I ATCGAT 1 cut(s) 272
BsaHI GRCGYC 2 cut(s) 186, 480
BsaJI CCNNGG 3 cut(s) 248, 249, 758
BsaWI WCCGGW 1 cut(s) 729
Bsc4I CCNNNNNNNGG 4 cut(s) 50, 254, 614, 918
Bse1I ACTGG 1 cut(s) 324
Bse21I CCTNAGG 1 cut(s) 564
Bse3DI GCAATG 1 cut(s) 137
BseBI CCWGG 2 cut(s) 85, 250
BseCI ATCGAT 1 cut(s) 272
BseDI CCNNGG 3 cut(s) 248, 249, 758
BseGI GGATG 5 cut(s) 246, 430, 499, 785, 904
BseLI CCNNNNNNNGG 4 cut(s) 50, 254, 614, 918
BseMI GCAATG 1 cut(s) 137
BseMII CTCAG 3 cut(s) 578, 623, 695
BseNI ACTGG 1 cut(s) 324
BseRI GAGGAG 2 cut(s) 552, 908
BseXI GCAGC 8 cut(s) 50, 360, 390, 402, 475, 718, 902, 975
BshFI GGCC 7 cut(s) 83, 247, 648, 661, 679, 764, 903
BshNI GGYRCC 1 cut(s) 776
BshVI ATCGAT 1 cut(s) 272
BsiSI CCGG 3 cut(s) 730, 859, 912
BslFI GGGAC 2 cut(s) 894, 967
BslI CCNNNNNNNGG 4 cut(s) 50, 254, 614, 918
BsmAI GTCTC 2 cut(s) 356, 980
BsmFI GGGAC 2 cut(s) 894, 967
BsnI GGCC 7 cut(s) 83, 247, 648, 661, 679, 764, 903
Bsp143I GATC 4 cut(s) 269, 273, 538, 1008
Bsp1720I GCTNAGC 2 cut(s) 702, 710
BspANI GGCC 7 cut(s) 83, 247, 648, 661, 679, 764, 903
BspCNI CTCAG 3 cut(s) 577, 622, 694
BspDI ATCGAT 1 cut(s) 272
BspLI GGNNCC 1 cut(s) 778
BspMAI CTGCAG 1 cut(s) 920
BspPI GGATC 3 cut(s) 268, 277, 533
BspT107I GGYRCC 1 cut(s) 776
BsrBI CCGCTC 1 cut(s) 208
BsrDI GCAATG 1 cut(s) 137
BsrI ACTGG 1 cut(s) 324
BssECI CCNNGG 3 cut(s) 248, 249, 758
BssMI GATC 4 cut(s) 269, 273, 538, 1008
BssNI GRCGYC 2 cut(s) 186, 480
BssSI CACGAG 2 cut(s) 790, 829
BssT1I CCWWGG 1 cut(s) 758
Bst2BI CACGAG 2 cut(s) 790, 829
Bst2UI CCWGG 2 cut(s) 85, 250
Bst4CI ACNGT 3 cut(s) 221, 317, 743
Bst6I CTCTTC 1 cut(s) 71
BstACI GRCGYC 2 cut(s) 186, 480
BstC8I GCNNGC 2 cut(s) 110, 716
BstDEI CTNAG 6 cut(s) 484, 564, 609, 681, 702, 710
BstF5I GGATG 5 cut(s) 246, 430, 499, 785, 904
BstH2I RGCGCY 2 cut(s) 27, 150
BstHHI GCGC 2 cut(s) 26, 149
BstKTI GATC 4 cut(s) 272, 276, 541, 1011
BstMAI GTCTC 2 cut(s) 356, 980
BstMBI GATC 4 cut(s) 269, 273, 538, 1008
BstNI CCWGG 2 cut(s) 85, 250
BstSCI CCNGG 3 cut(s) 83, 248, 910
BstSFI CTRYAG 1 cut(s) 916
BstV1I GCAGC 8 cut(s) 50, 360, 390, 402, 475, 718, 902, 975
Bsu15I ATCGAT 1 cut(s) 272
Bsu36I CCTNAGG 1 cut(s) 564
BsuRI GGCC 7 cut(s) 83, 247, 648, 661, 679, 764, 903
BsuTUI ATCGAT 1 cut(s) 272
BtsCI GGATG 5 cut(s) 246, 430, 499, 785, 904
BtsI GCAGTG 1 cut(s) 110
BtsIMutI CAGTG 2 cut(s) 110, 617
Cac8I GCNNGC 2 cut(s) 110, 716
CaiI CAGNNNCTG 1 cut(s) 688
CfoI GCGC 2 cut(s) 26, 149
Cfr13I GGNCC 3 cut(s) 81, 246, 762
ClaI ATCGAT 1 cut(s) 272
CseI GACGC 2 cut(s) 175, 488
CviAII CATG 8 cut(s) 35, 293, 513, 536, 650, 935, 965, 1006
DdeI CTNAG 6 cut(s) 484, 564, 609, 681, 702, 710
DpnI GATC 4 cut(s) 271, 275, 540, 1010
DpnII GATC 4 cut(s) 269, 273, 538, 1008
DrdI GACNNNNNNGTC 1 cut(s) 580
DseDI GACNNNNNNGTC 1 cut(s) 580
EaeI YGGCCR 2 cut(s) 646, 901
Eam1104I CTCTTC 1 cut(s) 71
EarI CTCTTC 1 cut(s) 71
Eco130I CCWWGG 1 cut(s) 758
Eco147I AGGCCT 1 cut(s) 661
Eco81I CCTNAGG 1 cut(s) 564
EcoRI GAATTC 1 cut(s) 721
EcoRII CCWGG 2 cut(s) 83, 248
EcoT14I CCWWGG 1 cut(s) 758
ErhI CCWWGG 1 cut(s) 758
FaeI CATG 8 cut(s) 38, 296, 516, 539, 653, 938, 968, 1009
FaqI GGGAC 2 cut(s) 894, 967
FatI CATG 8 cut(s) 34, 292, 512, 535, 649, 934, 964, 1005
FauI CCCGC 1 cut(s) 190
FbaI TGATCA 1 cut(s) 1008
FokI GGATG 5 cut(s) 253, 437, 506, 772, 911
FspBI CTAG 1 cut(s) 375
GlaI GCGC 2 cut(s) 25, 148
HaeII RGCGCY 2 cut(s) 27, 150
HaeIII GGCC 7 cut(s) 83, 247, 648, 661, 679, 764, 903
HapII CCGG 3 cut(s) 730, 859, 912
HgaI GACGC 2 cut(s) 175, 488
HhaI GCGC 2 cut(s) 26, 149
Hin1I GRCGYC 2 cut(s) 186, 480
Hin1II CATG 8 cut(s) 38, 296, 516, 539, 653, 938, 968, 1009
Hin6I GCGC 2 cut(s) 24, 147
HinP1I GCGC 2 cut(s) 24, 147
HindIII AAGCTT 1 cut(s) 633
HinfI GANTC 5 cut(s) 211, 308, 521, 685, 855
HpaII CCGG 3 cut(s) 730, 859, 912
HphI GGTGA 3 cut(s) 275, 281, 452
Hpy166II GTNNAC 1 cut(s) 257
Hpy188I TCNGA 5 cut(s) 10, 321, 361, 586, 684
Hpy188III TCNNGA 3 cut(s) 125, 518, 566
Hpy8I GTNNAC 1 cut(s) 257
Hpy99I CGWCG 2 cut(s) 126, 191
HpyAV CCTTC 1 cut(s) 425
HpyCH4III ACNGT 3 cut(s) 221, 317, 743
HpyCH4V TGCA 6 cut(s) 14, 38, 108, 168, 918, 964
HpyF3I CTNAG 6 cut(s) 484, 564, 609, 681, 702, 710
Hsp92I GRCGYC 2 cut(s) 186, 480
Hsp92II CATG 8 cut(s) 38, 296, 516, 539, 653, 938, 968, 1009
HspAI GCGC 2 cut(s) 24, 147
Ksp22I TGATCA 1 cut(s) 1008
Kzo9I GATC 4 cut(s) 269, 273, 538, 1008
LmnI GCTCC 1 cut(s) 127
Lsp1109I GCAGC 8 cut(s) 50, 360, 390, 402, 475, 718, 902, 975
LweI GCATC 1 cut(s) 658
MaeI CTAG 1 cut(s) 375
MaeIII GTNAC 1 cut(s) 440
MalI GATC 4 cut(s) 271, 275, 540, 1010
MbiI CCGCTC 1 cut(s) 208
MboI GATC 4 cut(s) 269, 273, 538, 1008
MboII GAAGA 5 cut(s) 82, 85, 88, 166, 821
MfeI CAATTG 1 cut(s) 543
MlsI TGGCCA 1 cut(s) 648
MluCI AATT 6 cut(s) 15, 95, 543, 598, 694, 721
MluNI TGGCCA 1 cut(s) 648
MlyI GAGTC 2 cut(s) 317, 694
MmeI TCCRAC 1 cut(s) 344
Mox20I TGGCCA 1 cut(s) 648
MscI TGGCCA 1 cut(s) 648
MseI TTAA 2 cut(s) 192, 1021
MslI CAYNNNNRTG 2 cut(s) 291, 790
Msp20I TGGCCA 1 cut(s) 648
MspA1I CMGCKG 4 cut(s) 51, 393, 906, 988
MspI CCGG 3 cut(s) 730, 859, 912
MspR9I CCNGG 3 cut(s) 85, 250, 912
MunI CAATTG 1 cut(s) 543
MvaI CCWGG 2 cut(s) 85, 250
NciI CCSGG 1 cut(s) 912
NdeII GATC 4 cut(s) 269, 273, 538, 1008
NlaIII CATG 8 cut(s) 38, 296, 516, 539, 653, 938, 968, 1009
NlaIV GGNNCC 1 cut(s) 778
NmuCI GTSAC 1 cut(s) 440
PasI CCCWGGG 1 cut(s) 249
PceI AGGCCT 1 cut(s) 661
PcsI WCGNNNNNNNCGW 1 cut(s) 317
PfeI GAWTC 3 cut(s) 211, 521, 855
PleI GAGTC 2 cut(s) 316, 693
PpsI GAGTC 2 cut(s) 316, 693
Psp6I CCWGG 2 cut(s) 83, 248
PspGI CCWGG 2 cut(s) 83, 248
PspN4I GGNNCC 1 cut(s) 778
PspPI GGNCC 3 cut(s) 81, 246, 762
PstI CTGCAG 1 cut(s) 920
PstNI CAGNNNCTG 1 cut(s) 688
PvuII CAGCTG 1 cut(s) 988
RseI CAYNNNNRTG 2 cut(s) 291, 790
SaqAI TTAA 2 cut(s) 192, 1021
Sau3AI GATC 4 cut(s) 269, 273, 538, 1008
Sau96I GGNCC 3 cut(s) 81, 246, 762
SchI GAGTC 2 cut(s) 317, 694
ScrFI CCNGG 3 cut(s) 85, 250, 912
SfaNI GCATC 1 cut(s) 658
SfcI CTRYAG 1 cut(s) 916
SmiMI CAYNNNNRTG 2 cut(s) 291, 790
SmlI CTYRAG 1 cut(s) 141
SmoI CTYRAG 1 cut(s) 141
Sse9I AATT 6 cut(s) 15, 95, 543, 598, 694, 721
SseBI AGGCCT 1 cut(s) 661
SspMI CTAG 1 cut(s) 375
StuI AGGCCT 1 cut(s) 661
StyD4I CCNGG 3 cut(s) 83, 248, 910
StyI CCWWGG 1 cut(s) 758
TaaI ACNGT 3 cut(s) 221, 317, 743
TaqI TCGA 3 cut(s) 114, 272, 311
TasI AATT 6 cut(s) 15, 95, 543, 598, 694, 721
TauI GCSGC 4 cut(s) 54, 57, 618, 906
TfiI GAWTC 3 cut(s) 211, 521, 855
Tru1I TTAA 2 cut(s) 192, 1021
Tru9I TTAA 2 cut(s) 192, 1021
TscAI CASTG 2 cut(s) 110, 617
TseFI GTSAC 1 cut(s) 440
TseI GCWGC 8 cut(s) 38, 348, 378, 390, 488, 706, 915, 988
Tsp45I GTSAC 1 cut(s) 440
TspDTI ATGAA 2 cut(s) 213, 822
TspGWI ACGGA 3 cut(s) 19, 282, 839
TspRI CASTG 2 cut(s) 110, 617
XapI RAATTY 2 cut(s) 598, 721
XspI CTAG 1 cut(s) 375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.