MD14G1188800.v1.1

RING/Ubox like zinc-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0005751
Physical Location & Seq
Reverse (-)
28049934 .. 28051366
1433 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1188800.v1.1.491

Sequence Viewer

Length: 1026 bp
ATGGTTTCCGATTCGATCACCAGAGCCTCCGTCCATGCGGTCCCAAACAATGGGGGCAGGGATTTGGGGAAGAAGAAGAGGGCCAGGTCTGCCAAATTGAAGCAGTGCAAGCTCGATGTTCGTCGGGAGCAATGGCTTTCTCAAGGCGCTATGAAGAACAAGGATTGCAAGGAGGAACAGAACAGTGTCGTTAAAGCAGGCAAAGAGCGGAATAACAACCGGTCATTTGGTAATCTGGAGATGAGGCCCAGAGGTGGACAGATTGATGGATCCATCCACCATCACCACCACCACCATGACAGTGATATGGAGTCGAACAGTCCGAACAGTTTGACAAGTAGTGTCTTGGAGAGCAACTATTCCGAGACTACCTTTACTAGCAGCAGCAGTAGTAGCAGCGGTGGCTGTTGCTCTGGAAATATCACAGAGGATGAGGAAGGTGGTGGCGATGATGGATGCTTGGACGATTGGGAAGCGGTGGCTGACGCCTTAGCTGCGGATGAGAAACCAAAAAATACATGTCCAGAATCGCCCCCAGAGCATGAACCAATTGCCCAAACGGTTTTTCCTCAGGAAACGGCTATTGGGTCTGATCAGGTCGAGCATTTGAACCCTCCATACAGCAGAGTTATCCCTAAAGCTTCAGGGAATGGCCATGCTTGGAGGCCTGATGATGCATTTCGGCCTCAGAGTCTGCCCAATTTGGCTAACCAGCTTAGCTTGCCGAATTCAAACCGTAAACATTACAGTTGTGGTGGTGTTCCTTGGGCCTATAACGGTGTTGTGCCTTCCTCATGTCCTATATGCTATGAAGATTTGGATTTCACAGACACGAGCTTTTTGCCTTGCTTGTGCGGATTCCGGCTCTGCCTTTTCTGCCACAATAGGATTATGGAGGAGGATGGTCGCTGTCCCGGCTGCAGGAAGCCATATAAGCATGAGCCTGTTGAGGCAGAGACAAGTGTGCATGGAGGAAGTCTCACATTTCGGCTGCCTCGTTCTTGTAGCATGATCACAAGGTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000077 GO:0000278 GO:0000288 GO:0000289 GO:0000956 GO:0002682 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006139 GO:0006401 GO:0006402 GO:0006464 GO:0006508 GO:0006511 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006977 GO:0007049 GO:0007093 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009892 GO:0009987 GO:0010468 GO:0010564 GO:0010605 GO:0010629 GO:0010948 GO:0016070 GO:0016071 GO:0016567 GO:0016740 GO:0019222 GO:0019439 GO:0019538 GO:0019787 GO:0019941 GO:0022402 GO:0023052 GO:0030163 GO:0030330 GO:0031570 GO:0031571 GO:0032446 GO:0033554 GO:0034641 GO:0034655 GO:0035556 GO:0036211 GO:0042770 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0044773 GO:0044774 GO:0044783 GO:0044819 GO:0045595 GO:0045637 GO:0045652 GO:0045786 GO:0045787 GO:0045930 GO:0046483 GO:0046700 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051603 GO:0051716 GO:0051726 GO:0051865 GO:0060255 GO:0065007 GO:0070647 GO:0071156 GO:0071158 GO:0071704 GO:0072331 GO:0072395 GO:0072401 GO:0072413 GO:0072422 GO:0072431 GO:0090068 GO:0090304 GO:0140096 GO:1901360 GO:1901361 GO:1901564 GO:1901565 GO:1901575 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902400 GO:1902402 GO:1902403 GO:1902806 GO:1902807 GO:1903047 GO:1903706 GO:2000026 GO:2000045 GO:2000134
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_4

Protein Analysis

342

Amino Acids

37.43

Weight (kDa)

6.1

Isoelectric Point (pI)

56.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_4 PF14570 266 - 312 4.5e-18 RING/Ubox like zinc-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 50
AccBSI CCGCTC 1 cut(s) 208
AciI CCGC 6 cut(s) 38, 208, 399, 476, 497, 855
AclWI GGATC 2 cut(s) 264, 277
AcoI YGGCCR 1 cut(s) 652
AcsI RAATTY 1 cut(s) 727
AcuI CTGAAG 1 cut(s) 627
AcyI GRCGYC 1 cut(s) 486
AfiI CCNNNNNNNGG 3 cut(s) 50, 254, 921
AflIII ACRYGT 1 cut(s) 518
AgeI ACCGGT 1 cut(s) 219
AgsI TTSAA 3 cut(s) 100, 610, 732
AjnI CCWGG 1 cut(s) 83
AjuI GAANNNNNNNTTGG 4 cut(s) 549, 567, 581, 599
AluBI AGCT 6 cut(s) 112, 494, 641, 715, 720, 837
AluI AGCT 6 cut(s) 112, 494, 641, 715, 720, 837
Alw26I GTCTC 3 cut(s) 359, 950, 983
AlwI GGATC 2 cut(s) 264, 277
AlwNI CAGNNNCTG 1 cut(s) 694
AoxI GGCC 6 cut(s) 81, 245, 652, 665, 683, 768
ApeKI GCWGC 6 cut(s) 381, 384, 396, 494, 918, 991
ApoI RAATTY 1 cut(s) 727
AsiGI ACCGGT 1 cut(s) 219
AspLEI GCGC 1 cut(s) 149
AspS9I GGNCC 5 cut(s) 40, 81, 246, 768, 1020
AsuC2I CCSGG 1 cut(s) 915
AsuHPI GGTGA 2 cut(s) 10, 275
AvaII GGWCC 2 cut(s) 40, 1020
AxyI CCTNAGG 1 cut(s) 570
BalI TGGCCA 1 cut(s) 654
BamHI GGATCC 1 cut(s) 269
BauI CACGAG 1 cut(s) 832
BbvI GCAGC 6 cut(s) 393, 396, 408, 481, 905, 978
BccI CCATC 5 cut(s) 260, 281, 288, 446, 896
BceAI ACGGC 1 cut(s) 594
BcgI CGANNNNNNTGC 2 cut(s) 823, 857
BciT130I CCWGG 1 cut(s) 85
BclI TGATCA 2 cut(s) 592, 1011
BcnI CCSGG 1 cut(s) 915
BcoDI GTCTC 3 cut(s) 359, 950, 983
BfaI CTAG 1 cut(s) 378
BfmI CTRYAG 1 cut(s) 919
BfoI RGCGCY 1 cut(s) 150
BisI GCNGC 6 cut(s) 382, 385, 397, 495, 919, 992
BlpI GCTNAGC 1 cut(s) 716
BlsI GCNGC 6 cut(s) 383, 386, 398, 496, 920, 993
Bme1390I CCNGG 2 cut(s) 85, 915
Bme18I GGWCC 2 cut(s) 40, 1020
BmgT120I GGNCC 5 cut(s) 40, 81, 246, 768, 1020
BmiI GGNNCC 2 cut(s) 42, 271
BmrFI CCNGG 2 cut(s) 85, 915
BmsI GCATC 2 cut(s) 446, 664
BplI GAGNNNNNCTC 2 cut(s) 963, 995
BpmI CTGGAG 1 cut(s) 257
Bpu10I CCTNAGC 1 cut(s) 490
Bpu1102I GCTNAGC 1 cut(s) 716
BpuEI CTTGAG 1 cut(s) 126
BpuMI CCSGG 1 cut(s) 915
BsaHI GRCGYC 1 cut(s) 486
BsaJI CCNNGG 1 cut(s) 764
BsaWI WCCGGW 1 cut(s) 219
Bsc4I CCNNNNNNNGG 3 cut(s) 50, 254, 921
Bse118I RCCGGY 1 cut(s) 219
Bse21I CCTNAGG 1 cut(s) 570
Bse3DI GCAATG 1 cut(s) 137
BseBI CCWGG 1 cut(s) 85
BseDI CCNNGG 1 cut(s) 764
BseGI GGATG 5 cut(s) 273, 436, 461, 505, 907
BseLI CCNNNNNNNGG 3 cut(s) 50, 254, 921
BseMI GCAATG 1 cut(s) 137
BseMII CTCAG 2 cut(s) 584, 701
BseRI GAGGAG 1 cut(s) 911
BseXI GCAGC 6 cut(s) 393, 396, 408, 481, 905, 978
BshFI GGCC 6 cut(s) 83, 247, 654, 667, 685, 770
BshTI ACCGGT 1 cut(s) 219
BsiSI CCGG 3 cut(s) 220, 862, 915
BslFI GGGAC 2 cut(s) 26, 897
BslI CCNNNNNNNGG 3 cut(s) 50, 254, 921
BsmAI GTCTC 3 cut(s) 359, 950, 983
BsmFI GGGAC 2 cut(s) 26, 897
BsnI GGCC 6 cut(s) 83, 247, 654, 667, 685, 770
Bsp143I GATC 4 cut(s) 15, 269, 592, 1011
Bsp1720I GCTNAGC 1 cut(s) 716
BspACI CCGC 6 cut(s) 38, 208, 399, 476, 497, 855
BspANI GGCC 6 cut(s) 83, 247, 654, 667, 685, 770
BspCNI CTCAG 2 cut(s) 583, 700
BspLI GGNNCC 2 cut(s) 42, 271
BspMAI CTGCAG 1 cut(s) 923
BspPI GGATC 2 cut(s) 264, 277
BsrBI CCGCTC 1 cut(s) 208
BsrDI GCAATG 1 cut(s) 137
BsrFI RCCGGY 1 cut(s) 219
BssAI RCCGGY 1 cut(s) 219
BssECI CCNNGG 1 cut(s) 764
BssMI GATC 4 cut(s) 15, 269, 592, 1011
BssNI GRCGYC 1 cut(s) 486
BssSI CACGAG 1 cut(s) 832
BssT1I CCWWGG 1 cut(s) 764
Bst2BI CACGAG 1 cut(s) 832
Bst2UI CCWGG 1 cut(s) 85
Bst4CI ACNGT 8 cut(s) 185, 302, 320, 329, 562, 737, 749, 779
Bst6I CTCTTC 1 cut(s) 71
BstACI GRCGYC 1 cut(s) 486
BstC8I GCNNGC 3 cut(s) 110, 199, 722
BstDEI CTNAG 4 cut(s) 490, 570, 687, 716
BstF5I GGATG 5 cut(s) 273, 436, 461, 505, 907
BstH2I RGCGCY 1 cut(s) 150
BstHHI GCGC 1 cut(s) 149
BstKTI GATC 4 cut(s) 18, 272, 595, 1014
BstMAI GTCTC 3 cut(s) 359, 950, 983
BstMBI GATC 4 cut(s) 15, 269, 592, 1011
BstNI CCWGG 1 cut(s) 85
BstNSI RCATGY 1 cut(s) 522
BstSCI CCNGG 2 cut(s) 83, 913
BstSFI CTRYAG 1 cut(s) 919
BstV1I GCAGC 6 cut(s) 393, 396, 408, 481, 905, 978
BstX2I RGATCY 1 cut(s) 269
BstYI RGATCY 1 cut(s) 269
Bsu36I CCTNAGG 1 cut(s) 570
BsuRI GGCC 6 cut(s) 83, 247, 654, 667, 685, 770
BtgZI GCGATG 1 cut(s) 462
BtsCI GGATG 5 cut(s) 273, 436, 461, 505, 907
BtsI GCAGTG 1 cut(s) 110
BtsIMutI CAGTG 3 cut(s) 110, 190, 307
Cac8I GCNNGC 3 cut(s) 110, 199, 722
CaiI CAGNNNCTG 1 cut(s) 694
CfoI GCGC 1 cut(s) 149
Cfr10I RCCGGY 1 cut(s) 219
Cfr13I GGNCC 5 cut(s) 40, 81, 246, 768, 1020
CseI GACGC 1 cut(s) 494
CspAI ACCGGT 1 cut(s) 219
CviAII CATG 9 cut(s) 35, 296, 519, 542, 656, 795, 938, 968, 1009
DdeI CTNAG 4 cut(s) 490, 570, 687, 716
DpnI GATC 4 cut(s) 17, 271, 594, 1013
DpnII GATC 4 cut(s) 15, 269, 592, 1011
EaeI YGGCCR 1 cut(s) 652
Eam1104I CTCTTC 1 cut(s) 71
EarI CTCTTC 1 cut(s) 71
Eco130I CCWWGG 1 cut(s) 764
Eco147I AGGCCT 1 cut(s) 667
Eco47I GGWCC 2 cut(s) 40, 1020
Eco57I CTGAAG 1 cut(s) 627
Eco81I CCTNAGG 1 cut(s) 570
EcoO109I RGGNCCY 1 cut(s) 1020
EcoRI GAATTC 1 cut(s) 727
EcoRII CCWGG 1 cut(s) 83
EcoT14I CCWWGG 1 cut(s) 764
EcoT22I ATGCAT 1 cut(s) 679
ErhI CCWWGG 1 cut(s) 764
FaeI CATG 9 cut(s) 38, 299, 522, 545, 659, 798, 941, 971, 1012
FaqI GGGAC 2 cut(s) 26, 897
FatI CATG 9 cut(s) 34, 295, 518, 541, 655, 794, 937, 967, 1008
FbaI TGATCA 2 cut(s) 592, 1011
Fnu4HI GCNGC 6 cut(s) 382, 385, 397, 495, 919, 992
FokI GGATG 5 cut(s) 260, 443, 468, 512, 914
Fsp4HI GCNGC 6 cut(s) 382, 385, 397, 495, 919, 992
FspBI CTAG 1 cut(s) 378
GlaI GCGC 1 cut(s) 148
GluI GCNGC 6 cut(s) 382, 385, 397, 495, 919, 992
GsuI CTGGAG 1 cut(s) 257
HaeII RGCGCY 1 cut(s) 150
HaeIII GGCC 6 cut(s) 83, 247, 654, 667, 685, 770
HapII CCGG 3 cut(s) 220, 862, 915
HgaI GACGC 1 cut(s) 494
HhaI GCGC 1 cut(s) 149
Hin1I GRCGYC 1 cut(s) 486
Hin1II CATG 9 cut(s) 38, 299, 522, 545, 659, 798, 941, 971, 1012
Hin6I GCGC 1 cut(s) 147
HinP1I GCGC 1 cut(s) 147
HindIII AAGCTT 1 cut(s) 639
HinfI GANTC 5 cut(s) 11, 311, 527, 691, 858
HpaII CCGG 3 cut(s) 220, 862, 915
HphI GGTGA 2 cut(s) 10, 275
Hpy166II GTNNAC 2 cut(s) 257, 740
Hpy188I TCNGA 5 cut(s) 10, 324, 364, 592, 690
Hpy188III TCNNGA 5 cut(s) 125, 236, 414, 524, 572
Hpy8I GTNNAC 2 cut(s) 257, 740
Hpy99I CGWCG 1 cut(s) 126
HpyAV CCTTC 2 cut(s) 431, 798
HpyCH4III ACNGT 8 cut(s) 185, 302, 320, 329, 562, 737, 749, 779
HpyCH4V TGCA 5 cut(s) 108, 168, 677, 921, 967
HpyF3I CTNAG 4 cut(s) 490, 570, 687, 716
Hsp92I GRCGYC 1 cut(s) 486
Hsp92II CATG 9 cut(s) 38, 299, 522, 545, 659, 798, 941, 971, 1012
HspAI GCGC 1 cut(s) 147
Ksp22I TGATCA 2 cut(s) 592, 1011
Kzo9I GATC 4 cut(s) 15, 269, 592, 1011
LmnI GCTCC 1 cut(s) 127
Lsp1109I GCAGC 6 cut(s) 393, 396, 408, 481, 905, 978
LweI GCATC 2 cut(s) 446, 664
MaeI CTAG 1 cut(s) 378
MalI GATC 4 cut(s) 17, 271, 594, 1013
MbiI CCGCTC 1 cut(s) 208
MboI GATC 4 cut(s) 15, 269, 592, 1011
MboII GAAGA 5 cut(s) 82, 85, 88, 166, 824
MfeI CAATTG 1 cut(s) 549
MflI RGATCY 1 cut(s) 269
MlsI TGGCCA 1 cut(s) 654
MluCI AATT 4 cut(s) 95, 549, 700, 727
MluNI TGGCCA 1 cut(s) 654
MlyI GAGTC 2 cut(s) 320, 700
Mox20I TGGCCA 1 cut(s) 654
Mph1103I ATGCAT 1 cut(s) 679
MscI TGGCCA 1 cut(s) 654
MseI TTAA 1 cut(s) 192
MslI CAYNNNNRTG 2 cut(s) 294, 300
Msp20I TGGCCA 1 cut(s) 654
MspA1I CMGCKG 1 cut(s) 399
MspI CCGG 3 cut(s) 220, 862, 915
MspR9I CCNGG 2 cut(s) 85, 915
MunI CAATTG 1 cut(s) 549
MvaI CCWGG 1 cut(s) 85
NciI CCSGG 1 cut(s) 915
NdeII GATC 4 cut(s) 15, 269, 592, 1011
NlaIII CATG 9 cut(s) 38, 299, 522, 545, 659, 798, 941, 971, 1012
NlaIV GGNNCC 2 cut(s) 42, 271
NsiI ATGCAT 1 cut(s) 679
NspI RCATGY 1 cut(s) 522
PceI AGGCCT 1 cut(s) 667
PciI ACATGT 1 cut(s) 518
PcsI WCGNNNNNNNCGW 2 cut(s) 320, 994
PfeI GAWTC 3 cut(s) 11, 527, 858
PflMI CCANNNNNTGG 1 cut(s) 50
PinAI ACCGGT 1 cut(s) 219
PkrI GCNGC 6 cut(s) 383, 386, 398, 496, 920, 993
PleI GAGTC 2 cut(s) 319, 699
PpsI GAGTC 2 cut(s) 319, 699
PpuMI RGGWCCY 1 cut(s) 1020
PscI ACATGT 1 cut(s) 518
Psp5II RGGWCCY 1 cut(s) 1020
Psp6I CCWGG 1 cut(s) 83
PspGI CCWGG 1 cut(s) 83
PspN4I GGNNCC 2 cut(s) 42, 271
PspPI GGNCC 5 cut(s) 40, 81, 246, 768, 1020
PspPPI RGGWCCY 1 cut(s) 1020
PsrI GAACNNNNNNTAC 2 cut(s) 602, 634
PstI CTGCAG 1 cut(s) 923
PstNI CAGNNNCTG 1 cut(s) 694
PsuI RGATCY 1 cut(s) 269
RseI CAYNNNNRTG 2 cut(s) 294, 300
SaqAI TTAA 1 cut(s) 192
SatI GCNGC 6 cut(s) 382, 385, 397, 495, 919, 992
Sau3AI GATC 4 cut(s) 15, 269, 592, 1011
Sau96I GGNCC 5 cut(s) 40, 81, 246, 768, 1020
SchI GAGTC 2 cut(s) 320, 700
ScrFI CCNGG 2 cut(s) 85, 915
SfaNI GCATC 2 cut(s) 446, 664
SfcI CTRYAG 1 cut(s) 919
SinI GGWCC 2 cut(s) 40, 1020
SmiMI CAYNNNNRTG 2 cut(s) 294, 300
SmlI CTYRAG 1 cut(s) 141
SmoI CTYRAG 1 cut(s) 141
Sse9I AATT 4 cut(s) 95, 549, 700, 727
SseBI AGGCCT 1 cut(s) 667
SsiI CCGC 6 cut(s) 38, 208, 399, 476, 497, 855
SspMI CTAG 1 cut(s) 378
StuI AGGCCT 1 cut(s) 667
StyD4I CCNGG 2 cut(s) 83, 913
StyI CCWWGG 1 cut(s) 764
TaaI ACNGT 8 cut(s) 185, 302, 320, 329, 562, 737, 749, 779
TaqI TCGA 4 cut(s) 14, 114, 314, 600
TasI AATT 4 cut(s) 95, 549, 700, 727
TfiI GAWTC 3 cut(s) 11, 527, 858
Tru1I TTAA 1 cut(s) 192
Tru9I TTAA 1 cut(s) 192
TscAI CASTG 3 cut(s) 110, 190, 307
TseI GCWGC 6 cut(s) 381, 384, 396, 494, 918, 991
TspDTI ATGAA 3 cut(s) 167, 558, 825
TspGWI ACGGA 1 cut(s) 19
TspRI CASTG 3 cut(s) 110, 190, 307
Van91I CCANNNNNTGG 1 cut(s) 50
VpaK11BI GGWCC 2 cut(s) 40, 1020
XapI RAATTY 1 cut(s) 727
XceI RCATGY 1 cut(s) 522
XspI CTAG 1 cut(s) 378
Zsp2I ATGCAT 1 cut(s) 679
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.