Prupe.5G181100_v2.0.a1

RING/Ubox like zinc-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0005751
Physical Location & Seq
Reverse (-)
15160807 .. 15165182
4376 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G181100.3

Sequence Viewer

Length: 1170 bp
ATGGTTTCCGATTCGATCACCAACGCTTCCATTCATGCAGCCCCAAACAACAACAACGCAGCCCGGGATGTGGGCAAGAAGAAGAGGGTGAGATTTCAATTTCCTTTTCCGGTTTACGATCTCTTGTTATTCTGTACACGAATTTGTATTTATTGGTTGATCTTTAGTAAAATTTCTCGTTTATTTGGGGATTTTCAGGCCAGGTCTGCCAAATTGAAGCAGTGCAAGCTCGATGTTCGTCGTGAGCAATGGCTTTCTCAAGGCGCTGTGAAGAATAAGGACTGTAAAGCGGAACAGAACGGCGTCGTTGAAGAACGCAAAGACCGTAATAACCACCATCCCTTCGGTAATCTAGGGATGAGGCCCGGAGGCGGTGAAAGTGACGGATCGATCCATCACCACCACCACCATGACAGCGATTTGGACTCAAACAGTCCGAACAGTTTGACAAGTAGCGTCTTGGGCAGCAACTATTCCGAGACTAACTTCACTGGGTCCAGCAGCAGCAGCAGCAGCAGCAGCAGCGGTGGCTGTTGCTCTGGAAATATCACAGAGGATGATGAAGGCGGTGGCGATGATGGGTGCTTGGACGATTGGGAAGCCGTGGCTGACGCCTTAGCCGCCAACGAGAAGCCGAAAAACCCATGTTTGGAGTCTCCCCCAGAGCATGATCCAATTGCTCAATCAGGTTCTCCTCAGGAAACGACTATTGGGTCTGATGGGCCGAGTTTTGGGGTTGAGAATTCGAAGCCTCAGTGCGCCAGAACTATCCCTAAAGCTTCAGGAAATGGCCTGGCATGGAGGCCTGATGATGCTTTTCGGCCTCAGAGTCTGCCCAATTTAGCTAAGCAGGTTAGCTTGCCAAATTCGAACCGGAAACATTACGGTTGTGGTGGTGTTCCTTGGGCCTATAGTGGTGTTGTTTCTGCGCCATCCTCATGTCCTATATGCTATGAAGATTTGGATTTCACGGACACAAGCTTTTTGCCATGCTTGTGCGGATTCCGGCTCTGCCTTTTCTGCCACAAGAGGATTCTCGAGGAGGATAGCCGCTGTCCTGGCTGCAGGAAGCCCTATGAGCATGAGCCTGTTGAGGCAGAGGCAAGTGTGCATGGAGGTAGCCTCACGTTTCGGCTGCCTCGTTCTTGTAGCCTGATCACAAGGTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000077 GO:0000278 GO:0000288 GO:0000289 GO:0000956 GO:0002682 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006139 GO:0006401 GO:0006402 GO:0006464 GO:0006508 GO:0006511 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006977 GO:0007049 GO:0007093 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009892 GO:0009987 GO:0010468 GO:0010564 GO:0010605 GO:0010629 GO:0010948 GO:0016070 GO:0016071 GO:0016567 GO:0016740 GO:0019222 GO:0019439 GO:0019538 GO:0019787 GO:0019941 GO:0022402 GO:0023052 GO:0030163 GO:0030330 GO:0031570 GO:0031571 GO:0032446 GO:0033554 GO:0034641 GO:0034655 GO:0035556 GO:0036211 GO:0042770 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0044773 GO:0044774 GO:0044783 GO:0044819 GO:0045595 GO:0045637 GO:0045652 GO:0045786 GO:0045787 GO:0045930 GO:0046483 GO:0046700 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051603 GO:0051716 GO:0051726 GO:0051865 GO:0060255 GO:0065007 GO:0070647 GO:0071156 GO:0071158 GO:0071704 GO:0072331 GO:0072395 GO:0072401 GO:0072413 GO:0072422 GO:0072431 GO:0090068 GO:0090304 GO:0140096 GO:1901360 GO:1901361 GO:1901564 GO:1901565 GO:1901575 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902400 GO:1902402 GO:1902403 GO:1902806 GO:1902807 GO:1903047 GO:1903706 GO:2000026 GO:2000045 GO:2000134
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_4

Protein Analysis

390

Amino Acids

42.31

Weight (kDa)

6.16

Isoelectric Point (pI)

55.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 712
Acc36I ACCTGC 1 cut(s) 841
AciI CCGC 7 cut(s) 290, 372, 525, 567, 621, 999, 1051
AclWI GGATC 3 cut(s) 385, 394, 665
AcsI RAATTY 4 cut(s) 141, 171, 742, 865
AcuI CTGAAG 1 cut(s) 765
AcyI GRCGYC 2 cut(s) 303, 612
AfaI GTAC 1 cut(s) 136
AfiI CCNNNNNNNGG 4 cut(s) 70, 371, 649, 731
AgsI TTSAA 3 cut(s) 98, 217, 311
AjnI CCWGG 3 cut(s) 200, 792, 1057
AjuI GAANNNNNNNTTGG 2 cut(s) 693, 725
AluBI AGCT 5 cut(s) 229, 779, 845, 858, 981
AluI AGCT 5 cut(s) 229, 779, 845, 858, 981
Alw26I GTCTC 2 cut(s) 473, 660
AlwI GGATC 3 cut(s) 385, 394, 665
AlwNI CAGNNNCTG 1 cut(s) 832
Ama87I CYCGRG 2 cut(s) 63, 1037
AoxI GGCC 7 cut(s) 198, 362, 722, 790, 803, 821, 906
ApoI RAATTY 4 cut(s) 141, 171, 742, 865
AspLEI GCGC 3 cut(s) 266, 761, 931
AspS9I GGNCC 5 cut(s) 363, 495, 722, 906, 1164
AsuC2I CCSGG 3 cut(s) 64, 65, 366
AsuHPI GGTGA 4 cut(s) 10, 100, 386, 389
AsuII TTCGAA 2 cut(s) 746, 869
AvaI CYCGRG 2 cut(s) 63, 1037
AvaII GGWCC 2 cut(s) 495, 1164
AxyI CCTNAGG 1 cut(s) 696
BccI CCATC 5 cut(s) 345, 402, 572, 713, 940
BceAI ACGGC 2 cut(s) 316, 587
BciT130I CCWGG 3 cut(s) 202, 794, 1059
BclI TGATCA 1 cut(s) 1155
BcnI CCSGG 3 cut(s) 64, 65, 366
BcoDI GTCTC 2 cut(s) 473, 660
BfaI CTAG 1 cut(s) 353
BfmI CTRYAG 2 cut(s) 910, 1063
BfoI RGCGCY 1 cut(s) 267
BfuAI ACCTGC 1 cut(s) 841
BlpI GCTNAGC 1 cut(s) 846
Bme1390I CCNGG 6 cut(s) 64, 65, 202, 366, 794, 1059
Bme18I GGWCC 2 cut(s) 495, 1164
BmeT110I CYCGRG 2 cut(s) 63, 1037
BmgT120I GGNCC 5 cut(s) 363, 495, 722, 906, 1164
BmiI GGNNCC 1 cut(s) 496
BmrFI CCNGG 6 cut(s) 64, 65, 202, 366, 794, 1059
BmrI ACTGGG 1 cut(s) 501
BmsI GCATC 1 cut(s) 802
BmuI ACTGGG 1 cut(s) 501
BplI GAGNNNNNCTC 2 cut(s) 1107, 1139
Bpu10I CCTNAGC 1 cut(s) 616
Bpu1102I GCTNAGC 1 cut(s) 846
Bpu14I TTCGAA 2 cut(s) 746, 869
BpuEI CTTGAG 1 cut(s) 243
BpuMI CCSGG 3 cut(s) 64, 65, 366
Bsa29I ATCGAT 1 cut(s) 389
BsaHI GRCGYC 2 cut(s) 303, 612
BsaJI CCNNGG 3 cut(s) 63, 603, 902
BsaWI WCCGGW 2 cut(s) 109, 873
Bsc4I CCNNNNNNNGG 4 cut(s) 70, 371, 649, 731
Bse1I ACTGG 1 cut(s) 496
Bse21I CCTNAGG 1 cut(s) 696
Bse3DI GCAATG 1 cut(s) 254
BseBI CCWGG 3 cut(s) 202, 794, 1059
BseCI ATCGAT 1 cut(s) 389
BseDI CCNNGG 3 cut(s) 63, 603, 902
BseGI GGATG 5 cut(s) 73, 337, 363, 562, 932
BseLI CCNNNNNNNGG 4 cut(s) 70, 371, 649, 731
BseMI GCAATG 1 cut(s) 254
BseMII CTCAG 3 cut(s) 710, 767, 839
BseNI ACTGG 1 cut(s) 496
BseRI GAGGAG 2 cut(s) 684, 1055
BshFI GGCC 7 cut(s) 200, 364, 724, 792, 805, 823, 908
BshVI ATCGAT 1 cut(s) 389
BsiHKCI CYCGRG 2 cut(s) 63, 1037
BsiSI CCGG 5 cut(s) 64, 110, 366, 874, 1006
BslI CCNNNNNNNGG 4 cut(s) 70, 371, 649, 731
BsmAI GTCTC 2 cut(s) 473, 660
BsnI GGCC 7 cut(s) 200, 364, 724, 792, 805, 823, 908
BsoBI CYCGRG 2 cut(s) 63, 1037
Bsp119I TTCGAA 2 cut(s) 746, 869
Bsp1407I TGTACA 1 cut(s) 134
Bsp143I GATC 7 cut(s) 15, 118, 159, 386, 390, 670, 1155
Bsp1720I GCTNAGC 1 cut(s) 846
BspACI CCGC 7 cut(s) 290, 372, 525, 567, 621, 999, 1051
BspANI GGCC 7 cut(s) 200, 364, 724, 792, 805, 823, 908
BspCNI CTCAG 3 cut(s) 709, 766, 838
BspDI ATCGAT 1 cut(s) 389
BspLI GGNNCC 1 cut(s) 496
BspMAI CTGCAG 1 cut(s) 1067
BspMI ACCTGC 1 cut(s) 841
BspPI GGATC 3 cut(s) 385, 394, 665
BspT104I TTCGAA 2 cut(s) 746, 869
BsrDI GCAATG 1 cut(s) 254
BsrGI TGTACA 1 cut(s) 134
BsrI ACTGG 1 cut(s) 496
BssECI CCNNGG 3 cut(s) 63, 603, 902
BssMI GATC 7 cut(s) 15, 118, 159, 386, 390, 670, 1155
BssNI GRCGYC 2 cut(s) 303, 612
BssT1I CCWWGG 1 cut(s) 902
Bst2UI CCWGG 3 cut(s) 202, 794, 1059
Bst4CI ACNGT 5 cut(s) 284, 326, 434, 443, 887
Bst6I CTCTTC 1 cut(s) 77
BstACI GRCGYC 2 cut(s) 303, 612
BstAUI TGTACA 1 cut(s) 134
BstBI TTCGAA 2 cut(s) 746, 869
BstC8I GCNNGC 2 cut(s) 227, 860
BstDEI CTNAG 5 cut(s) 616, 696, 753, 825, 846
BstDSI CCRYGG 1 cut(s) 603
BstF5I GGATG 5 cut(s) 73, 337, 363, 562, 932
BstH2I RGCGCY 1 cut(s) 267
BstHHI GCGC 3 cut(s) 266, 761, 931
BstKTI GATC 7 cut(s) 18, 121, 162, 389, 393, 673, 1158
BstMAI GTCTC 2 cut(s) 473, 660
BstMBI GATC 7 cut(s) 15, 118, 159, 386, 390, 670, 1155
BstNI CCWGG 3 cut(s) 202, 794, 1059
BstSCI CCNGG 6 cut(s) 62, 63, 200, 364, 792, 1057
BstSFI CTRYAG 2 cut(s) 910, 1063
Bsu15I ATCGAT 1 cut(s) 389
Bsu36I CCTNAGG 1 cut(s) 696
BsuRI GGCC 7 cut(s) 200, 364, 724, 792, 805, 823, 908
BsuTUI ATCGAT 1 cut(s) 389
BtgI CCRYGG 1 cut(s) 603
BtgZI GCGATG 1 cut(s) 588
BtsCI GGATG 5 cut(s) 73, 337, 363, 562, 932
BtsI GCAGTG 1 cut(s) 227
BtsIMutI CAGTG 3 cut(s) 227, 489, 761
BveI ACCTGC 1 cut(s) 841
Cac8I GCNNGC 2 cut(s) 227, 860
CaiI CAGNNNCTG 1 cut(s) 832
CfoI GCGC 3 cut(s) 266, 761, 931
Cfr13I GGNCC 5 cut(s) 363, 495, 722, 906, 1164
Cfr9I CCCGGG 1 cut(s) 63
ClaI ATCGAT 1 cut(s) 389
CseI GACGC 3 cut(s) 292, 445, 620
Csp6I GTAC 1 cut(s) 135
CviAII CATG 9 cut(s) 35, 410, 645, 668, 798, 939, 990, 1082, 1112
CviQI GTAC 1 cut(s) 135
DdeI CTNAG 5 cut(s) 616, 696, 753, 825, 846
DpnI GATC 7 cut(s) 17, 120, 161, 388, 392, 672, 1157
DpnII GATC 7 cut(s) 15, 118, 159, 386, 390, 670, 1155
DrdI GACNNNNNNGTC 1 cut(s) 712
DseDI GACNNNNNNGTC 1 cut(s) 712
Eam1104I CTCTTC 1 cut(s) 77
EarI CTCTTC 1 cut(s) 77
Eco130I CCWWGG 1 cut(s) 902
Eco147I AGGCCT 1 cut(s) 805
Eco47I GGWCC 2 cut(s) 495, 1164
Eco57I CTGAAG 1 cut(s) 765
Eco81I CCTNAGG 1 cut(s) 696
Eco88I CYCGRG 2 cut(s) 63, 1037
EcoO109I RGGNCCY 1 cut(s) 1164
EcoRI GAATTC 1 cut(s) 742
EcoRII CCWGG 3 cut(s) 200, 792, 1057
EcoT14I CCWWGG 1 cut(s) 902
ErhI CCWWGG 1 cut(s) 902
FaeI CATG 9 cut(s) 38, 413, 648, 671, 801, 942, 993, 1085, 1115
FatI CATG 9 cut(s) 34, 409, 644, 667, 797, 938, 989, 1081, 1111
FbaI TGATCA 1 cut(s) 1155
FokI GGATG 5 cut(s) 80, 324, 370, 569, 919
FspBI CTAG 1 cut(s) 353
GlaI GCGC 3 cut(s) 265, 760, 930
HaeII RGCGCY 1 cut(s) 267
HaeIII GGCC 7 cut(s) 200, 364, 724, 792, 805, 823, 908
HapII CCGG 5 cut(s) 64, 110, 366, 874, 1006
HgaI GACGC 3 cut(s) 292, 445, 620
HhaI GCGC 3 cut(s) 266, 761, 931
Hin1I GRCGYC 2 cut(s) 303, 612
Hin1II CATG 9 cut(s) 38, 413, 648, 671, 801, 942, 993, 1085, 1115
Hin6I GCGC 3 cut(s) 264, 759, 929
HinP1I GCGC 3 cut(s) 264, 759, 929
HindIII AAGCTT 2 cut(s) 777, 979
HinfI GANTC 6 cut(s) 11, 425, 653, 829, 1002, 1033
HpaII CCGG 5 cut(s) 64, 110, 366, 874, 1006
HphI GGTGA 4 cut(s) 10, 100, 386, 389
Hpy166II GTNNAC 2 cut(s) 115, 137
Hpy188I TCNGA 5 cut(s) 10, 438, 478, 718, 828
Hpy188III TCNNGA 5 cut(s) 242, 540, 698, 783, 1037
Hpy8I GTNNAC 2 cut(s) 115, 137
Hpy99I CGWCG 2 cut(s) 243, 308
HpyAV CCTTC 2 cut(s) 352, 557
HpyCH4III ACNGT 5 cut(s) 284, 326, 434, 443, 887
HpyCH4IV ACGT 1 cut(s) 1127
HpyCH4V TGCA 4 cut(s) 38, 225, 1065, 1111
HpyF3I CTNAG 5 cut(s) 616, 696, 753, 825, 846
HpySE526I ACGT 1 cut(s) 1127
Hsp92I GRCGYC 2 cut(s) 303, 612
Hsp92II CATG 9 cut(s) 38, 413, 648, 671, 801, 942, 993, 1085, 1115
HspAI GCGC 3 cut(s) 264, 759, 929
Ksp22I TGATCA 1 cut(s) 1155
Kzo9I GATC 7 cut(s) 15, 118, 159, 386, 390, 670, 1155
LweI GCATC 1 cut(s) 802
MaeI CTAG 1 cut(s) 353
MaeII ACGT 1 cut(s) 1127
MaeIII GTNAC 1 cut(s) 380
MalI GATC 7 cut(s) 17, 120, 161, 388, 392, 672, 1157
MboI GATC 7 cut(s) 15, 118, 159, 386, 390, 670, 1155
MboII GAAGA 5 cut(s) 91, 94, 283, 323, 968
MfeI CAATTG 1 cut(s) 675
MluCI AATT 8 cut(s) 98, 141, 171, 212, 675, 742, 838, 865
MlyI GAGTC 3 cut(s) 419, 662, 838
MslI CAYNNNNRTG 3 cut(s) 408, 937, 994
MspA1I CMGCKG 2 cut(s) 525, 1053
MspI CCGG 5 cut(s) 64, 110, 366, 874, 1006
MspR9I CCNGG 6 cut(s) 64, 65, 202, 366, 794, 1059
MunI CAATTG 1 cut(s) 675
MvaI CCWGG 3 cut(s) 202, 794, 1059
NciI CCSGG 3 cut(s) 64, 65, 366
NdeII GATC 7 cut(s) 15, 118, 159, 386, 390, 670, 1155
NlaIII CATG 9 cut(s) 38, 413, 648, 671, 801, 942, 993, 1085, 1115
NlaIV GGNNCC 1 cut(s) 496
NmeAIII GCCGAG 1 cut(s) 750
NmuCI GTSAC 1 cut(s) 380
NspV TTCGAA 2 cut(s) 746, 869
PaeR7I CTCGAG 1 cut(s) 1037
PceI AGGCCT 1 cut(s) 805
PcsI WCGNNNNNNNCGW 2 cut(s) 322, 1138
PfeI GAWTC 3 cut(s) 11, 1002, 1033
PleI GAGTC 3 cut(s) 419, 661, 837
PpsI GAGTC 3 cut(s) 419, 661, 837
PpuMI RGGWCCY 1 cut(s) 1164
Psp5II RGGWCCY 1 cut(s) 1164
Psp6I CCWGG 3 cut(s) 200, 792, 1057
PspGI CCWGG 3 cut(s) 200, 792, 1057
PspN4I GGNNCC 1 cut(s) 496
PspPI GGNCC 5 cut(s) 363, 495, 722, 906, 1164
PspPPI RGGWCCY 1 cut(s) 1164
PstI CTGCAG 1 cut(s) 1067
PstNI CAGNNNCTG 1 cut(s) 832
RsaI GTAC 1 cut(s) 136
RsaNI GTAC 1 cut(s) 135
RseI CAYNNNNRTG 3 cut(s) 408, 937, 994
Sau3AI GATC 7 cut(s) 15, 118, 159, 386, 390, 670, 1155
Sau96I GGNCC 5 cut(s) 363, 495, 722, 906, 1164
SchI GAGTC 3 cut(s) 419, 662, 838
ScrFI CCNGG 6 cut(s) 64, 65, 202, 366, 794, 1059
SfaNI GCATC 1 cut(s) 802
SfcI CTRYAG 2 cut(s) 910, 1063
Sfr274I CTCGAG 1 cut(s) 1037
SfuI TTCGAA 2 cut(s) 746, 869
SinI GGWCC 2 cut(s) 495, 1164
SlaI CTCGAG 1 cut(s) 1037
SmaI CCCGGG 1 cut(s) 65
SmiMI CAYNNNNRTG 3 cut(s) 408, 937, 994
SmlI CTYRAG 2 cut(s) 258, 1037
SmoI CTYRAG 2 cut(s) 258, 1037
Sse9I AATT 8 cut(s) 98, 141, 171, 212, 675, 742, 838, 865
SseBI AGGCCT 1 cut(s) 805
SsiI CCGC 7 cut(s) 290, 372, 525, 567, 621, 999, 1051
SspMI CTAG 1 cut(s) 353
StuI AGGCCT 1 cut(s) 805
StyD4I CCNGG 6 cut(s) 62, 63, 200, 364, 792, 1057
StyI CCWWGG 1 cut(s) 902
TaaI ACNGT 5 cut(s) 284, 326, 434, 443, 887
TaiI ACGT 1 cut(s) 1130
TaqI TCGA 6 cut(s) 14, 231, 389, 746, 869, 1038
TasI AATT 8 cut(s) 98, 141, 171, 212, 675, 742, 838, 865
TatI WGTACW 1 cut(s) 134
TauI GCSGC 2 cut(s) 623, 1053
TfiI GAWTC 3 cut(s) 11, 1002, 1033
TscAI CASTG 3 cut(s) 227, 496, 761
TseFI GTSAC 1 cut(s) 380
Tsp45I GTSAC 1 cut(s) 380
TspDTI ATGAA 3 cut(s) 23, 576, 969
TspGWI ACGGA 2 cut(s) 399, 986
TspMI CCCGGG 1 cut(s) 63
TspRI CASTG 3 cut(s) 227, 496, 761
VpaK11BI GGWCC 2 cut(s) 495, 1164
XapI RAATTY 4 cut(s) 141, 171, 742, 865
XhoI CTCGAG 1 cut(s) 1037
XmaI CCCGGG 1 cut(s) 63
XspI CTAG 1 cut(s) 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.