MD06G1196100.v1.1
NAC Family

NAC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0008227
Physical Location & Seq
Forward (+)
33005854 .. 33007989
2136 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1196100.v1.1.491

Sequence Viewer

Length: 1086 bp
ATGGAAAACGTTCCAGCTGGGATTGTTGAGGAGGAGGATCATATCGATTTGCCGCCAGGATTCCGATTCCATCCAACCGATGAAGAGCTTATTTCTCACTACTTGCACAAGAAGGTGATCGATACCAGCTTCTCTTGTAAAGCAATTGGGGAGGTGGACTTGAACAAGTCGGAGCCTTGGGAATTGCCTTGGAAAGCGAAAATGGGAGAAAAGAAGTGGTACTTTTTTTGTGTGAGAGACAGAAAGTACCCAACTGGTCTGAGGACAAACAGGGCGACAGAAGCCGGATACTGGAAAGCTACAGGGAAGGATAAGGAGATTTACAGAGGAAAATTTCTGGTTGGGATGAAGAAAACCTTGGTTTTCTACAAAGGAAGAGCTCCCAAAGGAGAAAAAAGTAACTGGGTGATCCATGAATATAGATTGGATGGTAAATTCTCTGTCCACAACCTCCCCAAAACTGCAAAGAATGAATGGGTGATCTGCAGGGTTTTTGAAAAGAATTCTGGTGGCAAGAAAATCCATATTTCTGGATTTGTGAGTGTGGGTTCTCTCGGAAATGAAATCGGTCCTTCTGGCTTACCGCCATTGATGGATTCTTCGCCCTATAGCGCCGCCAAGGCCAAACCTGCTTCCGAGTCCCATTACGTGCCCTGCTTCTCCAACCCTGATCCCACTGATGTTCAAAGAAACCAAGGGAATATTTTTGATTACATGAACAACAATACTCTCTTTGGTGTTTCTTCAAACACTTCCAACTCTTTCCCAATAATCCCATTTCCCAATTCATTCTACTCTGCTCATAATGCTCCAATTTCGCCGAATTTTCAGTTCCCGTGTTCAAATTTACTGCAAGATCAGTCCATCTTGCGGGCCTTGTTTGAAAACAATGCAGCAAACATGAGGCAGCATAGCTTCAAAACAGAGAGAGAAATGGTGAGTGTGTCCCAAGAAACCGGCCTCACAAGCGATATGAACACTGAAATCTCTTCAGTCATGTCAAATCTTGACATGTCAAGAAGGCCATTTGGTGATCAAGATCCTCCAGCTGCAGCAGCAGGCCTGGATAATTTTTGGAATTATTAA

Protein Analysis

362

Amino Acids

40.71

Weight (kDa)

7.66

Isoelectric Point (pI)

47.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 18 - 142 7.1e-48 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 637
AciI CCGC 4 cut(s) 53, 584, 615, 871
AclI AACGTT 1 cut(s) 9
AclWI GGATC 4 cut(s) 45, 403, 665, 1034
AcsI RAATTY 5 cut(s) 332, 434, 502, 823, 844
AcuI CTGAAG 1 cut(s) 975
AfaI GTAC 2 cut(s) 221, 248
AfiI CCNNNNNNNGG 2 cut(s) 291, 870
AflIII ACRYGT 1 cut(s) 1011
AgsI TTSAA 7 cut(s) 163, 497, 686, 747, 843, 884, 919
AjnI CCWGG 2 cut(s) 55, 1062
AjuI GAANNNNNNNTTGG 2 cut(s) 341, 373
AluBI AGCT 7 cut(s) 17, 88, 129, 299, 380, 915, 1049
AluI AGCT 7 cut(s) 17, 88, 129, 299, 380, 915, 1049
Alw21I GWGCWC 1 cut(s) 382
Alw26I GTCTC 1 cut(s) 231
AlwI GGATC 4 cut(s) 45, 403, 665, 1034
AoxI GGCC 5 cut(s) 621, 873, 958, 1022, 1060
ApeKI GCWGC 5 cut(s) 893, 907, 1049, 1052, 1055
ApoI RAATTY 5 cut(s) 332, 434, 502, 823, 844
Asp700I GAANNNNTTC 1 cut(s) 9
AspLEI GCGC 1 cut(s) 614
AspS9I GGNCC 2 cut(s) 569, 873
AsuHPI GGTGA 5 cut(s) 127, 418, 490, 949, 1043
AvaII GGWCC 1 cut(s) 569
BaeGI GKGCMC 1 cut(s) 654
BanII GRGCYC 1 cut(s) 382
Bbv12I GWGCWC 1 cut(s) 382
BbvI GCAGC 5 cut(s) 905, 919, 1036, 1064, 1067
BccI CCATC 4 cut(s) 78, 422, 586, 872
BciT130I CCWGG 2 cut(s) 57, 1064
BciVI GTATCC 1 cut(s) 281
BclI TGATCA 1 cut(s) 1033
BcoDI GTCTC 1 cut(s) 231
BfmI CTRYAG 4 cut(s) 300, 484, 607, 1050
BfoI RGCGCY 1 cut(s) 615
BfuAI ACCTGC 1 cut(s) 637
BfuI GTATCC 1 cut(s) 281
BglI GCCNNNNNGGC 1 cut(s) 620
BisI GCNGC 7 cut(s) 53, 615, 894, 908, 1050, 1053, 1056
BlsI GCNGC 7 cut(s) 54, 616, 895, 909, 1051, 1054, 1057
Bme1390I CCNGG 2 cut(s) 57, 1064
Bme18I GGWCC 1 cut(s) 569
BmgT120I GGNCC 2 cut(s) 569, 873
BmiI GGNNCC 1 cut(s) 174
BmrFI CCNGG 2 cut(s) 57, 1064
BmrI ACTGGG 1 cut(s) 412
BmuI ACTGGG 1 cut(s) 412
BpmI CTGGAG 1 cut(s) 1029
Bsa29I ATCGAT 2 cut(s) 45, 120
BsaAI YACGTR 1 cut(s) 649
BsaBI GATNNNNATC 1 cut(s) 1038
BsaJI CCNNGG 5 cut(s) 176, 188, 357, 618, 694
Bsc4I CCNNNNNNNGG 2 cut(s) 291, 870
Bse118I RCCGGY 1 cut(s) 956
Bse1I ACTGG 3 cut(s) 259, 296, 407
Bse8I GATNNNNATC 1 cut(s) 1038
BseBI CCWGG 2 cut(s) 57, 1064
BseCI ATCGAT 2 cut(s) 45, 120
BseDI CCNNGG 5 cut(s) 176, 188, 357, 618, 694
BseGI GGATG 3 cut(s) 70, 351, 433
BseJI GATNNNNATC 1 cut(s) 1038
BseLI CCNNNNNNNGG 2 cut(s) 291, 870
BseMII CTCAG 1 cut(s) 251
BseNI ACTGG 3 cut(s) 259, 296, 407
BseRI GAGGAG 2 cut(s) 44, 47
BseSI GKGCMC 1 cut(s) 654
BseXI GCAGC 5 cut(s) 905, 919, 1036, 1064, 1067
BseYI CCCAGC 1 cut(s) 17
BshFI GGCC 5 cut(s) 623, 875, 960, 1024, 1062
BshVI ATCGAT 2 cut(s) 45, 120
BsiHKAI GWGCWC 1 cut(s) 382
BsiSI CCGG 2 cut(s) 285, 957
BslFI GGGAC 2 cut(s) 625, 931
BslI CCNNNNNNNGG 2 cut(s) 291, 870
BsmAI GTCTC 1 cut(s) 231
BsmFI GGGAC 2 cut(s) 625, 931
BsnI GGCC 5 cut(s) 623, 875, 960, 1024, 1062
Bsp1286I GDGCHC 2 cut(s) 382, 654
Bsp143I GATC 8 cut(s) 37, 117, 408, 480, 670, 856, 1033, 1039
BspACI CCGC 4 cut(s) 53, 584, 615, 871
BspANI GGCC 5 cut(s) 623, 875, 960, 1024, 1062
BspCNI CTCAG 1 cut(s) 252
BspDI ATCGAT 2 cut(s) 45, 120
BspLI GGNNCC 1 cut(s) 174
BspMAI CTGCAG 2 cut(s) 488, 1054
BspMI ACCTGC 1 cut(s) 637
BspPI GGATC 4 cut(s) 45, 403, 665, 1034
BspQI GCTCTTC 2 cut(s) 78, 370
BsrFI RCCGGY 1 cut(s) 956
BsrI ACTGG 3 cut(s) 259, 296, 407
BssAI RCCGGY 1 cut(s) 956
BssECI CCNNGG 5 cut(s) 176, 188, 357, 618, 694
BssMI GATC 8 cut(s) 37, 117, 408, 480, 670, 856, 1033, 1039
BssT1I CCWWGG 5 cut(s) 176, 188, 357, 618, 694
Bst2UI CCWGG 2 cut(s) 57, 1064
Bst6I CTCTTC 3 cut(s) 78, 370, 994
BstBAI YACGTR 1 cut(s) 649
BstC8I GCNNGC 2 cut(s) 873, 1060
BstDEI CTNAG 1 cut(s) 260
BstF5I GGATG 3 cut(s) 70, 351, 433
BstH2I RGCGCY 1 cut(s) 615
BstHHI GCGC 1 cut(s) 614
BstKTI GATC 8 cut(s) 40, 120, 411, 483, 673, 859, 1036, 1042
BstMAI GTCTC 1 cut(s) 231
BstMBI GATC 8 cut(s) 37, 117, 408, 480, 670, 856, 1033, 1039
BstMWI GCNNNNNNNGC 6 cut(s) 281, 620, 629, 806, 966, 1055
BstNI CCWGG 2 cut(s) 57, 1064
BstNSI RCATGY 1 cut(s) 1015
BstSCI CCNGG 2 cut(s) 55, 1062
BstSFI CTRYAG 4 cut(s) 300, 484, 607, 1050
BstSLI GKGCMC 1 cut(s) 654
BstV1I GCAGC 5 cut(s) 905, 919, 1036, 1064, 1067
BstX2I RGATCY 1 cut(s) 1039
BstXI CCANNNNNNTGG 1 cut(s) 530
BstYI RGATCY 1 cut(s) 1039
Bsu15I ATCGAT 2 cut(s) 45, 120
BsuI GTATCC 1 cut(s) 281
BsuRI GGCC 5 cut(s) 623, 875, 960, 1024, 1062
BsuTUI ATCGAT 2 cut(s) 45, 120
BtsCI GGATG 3 cut(s) 70, 351, 433
BtsIMutI CAGTG 2 cut(s) 675, 978
BveI ACCTGC 1 cut(s) 637
Cac8I GCNNGC 2 cut(s) 873, 1060
CfoI GCGC 1 cut(s) 614
Cfr10I RCCGGY 1 cut(s) 956
Cfr13I GGNCC 2 cut(s) 569, 873
ClaI ATCGAT 2 cut(s) 45, 120
Csp6I GTAC 2 cut(s) 220, 247
CviAII CATG 5 cut(s) 413, 715, 901, 997, 1012
CviQI GTAC 2 cut(s) 220, 247
DdeI CTNAG 1 cut(s) 260
DpnI GATC 8 cut(s) 39, 119, 410, 482, 672, 858, 1035, 1041
DpnII GATC 8 cut(s) 37, 117, 408, 480, 670, 856, 1033, 1039
Eam1104I CTCTTC 3 cut(s) 78, 370, 994
EarI CTCTTC 3 cut(s) 78, 370, 994
Ecl136II GAGCTC 1 cut(s) 380
Eco130I CCWWGG 5 cut(s) 176, 188, 357, 618, 694
Eco147I AGGCCT 1 cut(s) 1062
Eco24I GRGCYC 1 cut(s) 382
Eco47I GGWCC 1 cut(s) 569
Eco53kI GAGCTC 1 cut(s) 380
Eco57I CTGAAG 1 cut(s) 975
EcoICRI GAGCTC 1 cut(s) 380
EcoRI GAATTC 1 cut(s) 502
EcoRII CCWGG 2 cut(s) 55, 1062
EcoT14I CCWWGG 5 cut(s) 176, 188, 357, 618, 694
EcoT38I GRGCYC 1 cut(s) 382
ErhI CCWWGG 5 cut(s) 176, 188, 357, 618, 694
FaeI CATG 5 cut(s) 416, 718, 904, 1000, 1015
FalI AAGNNNNNCTT 4 cut(s) 206, 238, 341, 373
FaqI GGGAC 2 cut(s) 625, 931
FatI CATG 5 cut(s) 412, 714, 900, 996, 1011
FauI CCCGC 1 cut(s) 864
FbaI TGATCA 1 cut(s) 1033
Fnu4HI GCNGC 7 cut(s) 53, 615, 894, 908, 1050, 1053, 1056
FokI GGATG 3 cut(s) 57, 358, 440
FriOI GRGCYC 1 cut(s) 382
Fsp4HI GCNGC 7 cut(s) 53, 615, 894, 908, 1050, 1053, 1056
GlaI GCGC 1 cut(s) 613
GluI GCNGC 7 cut(s) 53, 615, 894, 908, 1050, 1053, 1056
GsaI CCCAGC 1 cut(s) 21
GsuI CTGGAG 1 cut(s) 1029
HaeII RGCGCY 1 cut(s) 615
HaeIII GGCC 5 cut(s) 623, 875, 960, 1024, 1062
HapII CCGG 2 cut(s) 285, 957
HhaI GCGC 1 cut(s) 614
Hin1II CATG 5 cut(s) 416, 718, 904, 1000, 1015
Hin6I GCGC 1 cut(s) 612
HinP1I GCGC 1 cut(s) 612
HinfI GANTC 4 cut(s) 60, 66, 596, 638
HpaII CCGG 2 cut(s) 285, 957
HphI GGTGA 5 cut(s) 127, 418, 490, 949, 1043
Hpy166II GTNNAC 2 cut(s) 157, 445
Hpy188I TCNGA 5 cut(s) 65, 172, 261, 557, 637
Hpy188III TCNNGA 4 cut(s) 531, 1007, 1017, 1037
Hpy8I GTNNAC 2 cut(s) 157, 445
HpyAV CCTTC 4 cut(s) 106, 301, 582, 1014
HpyCH4IV ACGT 2 cut(s) 9, 648
HpyCH4V TGCA 6 cut(s) 106, 464, 486, 853, 893, 1052
HpyF10VI GCNNNNNNNGC 6 cut(s) 281, 620, 629, 806, 966, 1055
HpyF3I CTNAG 1 cut(s) 260
HpySE526I ACGT 2 cut(s) 9, 648
Hsp92II CATG 5 cut(s) 416, 718, 904, 1000, 1015
HspAI GCGC 1 cut(s) 612
Ksp22I TGATCA 1 cut(s) 1033
Kzo9I GATC 8 cut(s) 37, 117, 408, 480, 670, 856, 1033, 1039
LguI GCTCTTC 2 cut(s) 78, 370
LmnI GCTCC 3 cut(s) 172, 385, 814
Lsp1109I GCAGC 5 cut(s) 905, 919, 1036, 1064, 1067
MaeII ACGT 2 cut(s) 9, 648
MaeIII GTNAC 1 cut(s) 398
MalI GATC 8 cut(s) 39, 119, 410, 482, 672, 858, 1035, 1041
MboI GATC 8 cut(s) 37, 117, 408, 480, 670, 856, 1033, 1039
MboII GAAGA 6 cut(s) 95, 361, 387, 591, 735, 981
MfeI CAATTG 1 cut(s) 144
MflI RGATCY 1 cut(s) 1039
MhlI GDGCHC 2 cut(s) 382, 654
MlyI GAGTC 1 cut(s) 647
MmeI TCCRAC 4 cut(s) 98, 150, 687, 780
MroXI GAANNNNTTC 1 cut(s) 9
MseI TTAA 1 cut(s) 1084
MspA1I CMGCKG 2 cut(s) 17, 1049
MspI CCGG 2 cut(s) 285, 957
MspR9I CCNGG 2 cut(s) 57, 1064
MunI CAATTG 1 cut(s) 144
MvaI CCWGG 2 cut(s) 57, 1064
MwoI GCNNNNNNNGC 6 cut(s) 281, 620, 629, 806, 966, 1055
NdeII GATC 8 cut(s) 37, 117, 408, 480, 670, 856, 1033, 1039
NlaIII CATG 5 cut(s) 416, 718, 904, 1000, 1015
NlaIV GGNNCC 1 cut(s) 174
NspI RCATGY 1 cut(s) 1015
PceI AGGCCT 1 cut(s) 1062
PciI ACATGT 1 cut(s) 1011
PciSI GCTCTTC 2 cut(s) 78, 370
PdmI GAANNNNTTC 1 cut(s) 9
PfeI GAWTC 3 cut(s) 60, 66, 596
PkrI GCNGC 7 cut(s) 54, 616, 895, 909, 1051, 1054, 1057
PleI GAGTC 1 cut(s) 646
PpsI GAGTC 1 cut(s) 646
Ppu21I YACGTR 1 cut(s) 649
PscI ACATGT 1 cut(s) 1011
Psp124BI GAGCTC 1 cut(s) 382
Psp1406I AACGTT 1 cut(s) 9
Psp6I CCWGG 2 cut(s) 55, 1062
PspFI CCCAGC 1 cut(s) 17
PspGI CCWGG 2 cut(s) 55, 1062
PspN4I GGNNCC 1 cut(s) 174
PspPI GGNCC 2 cut(s) 569, 873
PstI CTGCAG 2 cut(s) 488, 1054
PsuI RGATCY 1 cut(s) 1039
PvuII CAGCTG 2 cut(s) 17, 1049
RsaI GTAC 2 cut(s) 221, 248
RsaNI GTAC 2 cut(s) 220, 247
SacI GAGCTC 1 cut(s) 382
SapI GCTCTTC 2 cut(s) 78, 370
SaqAI TTAA 1 cut(s) 1084
SatI GCNGC 7 cut(s) 53, 615, 894, 908, 1050, 1053, 1056
Sau3AI GATC 8 cut(s) 37, 117, 408, 480, 670, 856, 1033, 1039
Sau96I GGNCC 2 cut(s) 569, 873
SchI GAGTC 1 cut(s) 647
ScrFI CCNGG 2 cut(s) 57, 1064
SduI GDGCHC 2 cut(s) 382, 654
SfcI CTRYAG 4 cut(s) 300, 484, 607, 1050
SinI GGWCC 1 cut(s) 569
SseBI AGGCCT 1 cut(s) 1062
SsiI CCGC 4 cut(s) 53, 584, 615, 871
SspI AATATT 1 cut(s) 703
SstI GAGCTC 1 cut(s) 382
StuI AGGCCT 1 cut(s) 1062
StyD4I CCNGG 2 cut(s) 55, 1062
StyI CCWWGG 5 cut(s) 176, 188, 357, 618, 694
TaiI ACGT 2 cut(s) 12, 651
TaqI TCGA 2 cut(s) 45, 120
TaqII GACCGA 1 cut(s) 557
TauI GCSGC 2 cut(s) 55, 617
TfiI GAWTC 3 cut(s) 60, 66, 596
Tru1I TTAA 1 cut(s) 1084
Tru9I TTAA 1 cut(s) 1084
TscAI CASTG 2 cut(s) 682, 985
TseI GCWGC 5 cut(s) 893, 907, 1049, 1052, 1055
TspDTI ATGAA 8 cut(s) 96, 362, 429, 486, 576, 731, 777, 989
TspRI CASTG 2 cut(s) 682, 985
VpaK11BI GGWCC 1 cut(s) 569
XapI RAATTY 5 cut(s) 332, 434, 502, 823, 844
XceI RCATGY 1 cut(s) 1015
XmnI GAANNNNTTC 1 cut(s) 9
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.