MD14G1203000.v1.1
NAC Family

NAC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0008227
Physical Location & Seq
Forward (+)
29132980 .. 29135232
2253 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1203000.v1.1.491

Sequence Viewer

Length: 1086 bp
ATGGAAAACGTTCCAGCTGGGATTGTTGAGGAGGATGATCAGATCGATTTGCCACCCGGGTTTCGCTTCCATCCAACCGATGAAGAGCTTATTTCCCACTACTTGCACAAGAAGGTTATTGATACCAGCTTCTCTTGTAAAGCCATGGGGGAGGTGGACTTGAACAAGTCAGAGCCTTGGCAATTGCCTCGGAAAGCGAAAATGGGAGAAAAGGAGTGGTACTTTTTCTGTGTGAGAGACAGAAAGTACCCAACTGGTCTGAGGACTAACAGGGCGACCGAAGCCGGATACTGGAAGGCCACAGGGAAAGATAAGGAGATCTACAGTACAAAATCTCTGGTTGGGATGAAGAAAACCTTGGTTTTCTACAAAGGAAGAGCCCCGAAAGGAGAAAAAAGTAACTGGGTTATGCATGAATATAGATTGGATGGTAAATTCTCTGTTCACAACCTCCCCAAAACTGCAAAGAATGAATGGGTGATTTGCAGAGTTTTTGAAAAGAATTCTGGTGGCAAGAAAACCCATATTTCTGGATTTGTGACTTTGGCTTCTCTCGGAAATGAAATTGGTTCTTCTGGCCTACCACCATTGATGGATTCTTCGCCCTACAGCGCTCCCAAGGCCAAACCTGCTTCTGAGTCCCATTACGTGCCCTGCTTCTCCAACCCTGATCCCACTGATGTTCAAAGAAACCAAGGGAATATTGCTGATTACATGAACAACAATCCTCTCTTTGGTGTTTCTTCAAACTCTTCCAACTTTTTCCCAAGAATCCCATTTCCCAATTCATTCTACTCTGCTCATAATGCTCCCCCTTCGACAAGTTTCAAATTCCCCGGTTCGGATATACTGCAAGATCAGTCCATCCTGAGGGCCTTGTTAGAAAACAATGCTGCAAACATGAGGCAGCAGAGCTACAAAACAGAGAGGGAACTAGTGAGTGTGTCCCAAGAAACCGGCCTCACAAGCGATATGAACGCTGAAATCTCTTCAGTCATGTCCAATCTTGAAATGGCAACAAGGCCATATGGTGATCAAGATCCTCCAGCTGCAGCAGCAGGCCTGGATAATTTTTGGAATTATTAA

Protein Analysis

362

Amino Acids

40.47

Weight (kDa)

7.09

Isoelectric Point (pI)

46.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 18 - 142 2.1e-49 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 637
AclI AACGTT 1 cut(s) 9
AclWI GGATC 2 cut(s) 665, 1034
AcsI RAATTY 3 cut(s) 434, 502, 830
AcuI CTGAAG 1 cut(s) 975
AfaI GTAC 3 cut(s) 221, 248, 328
AfeI AGCGCT 1 cut(s) 613
AfiI CCNNNNNNNGG 4 cut(s) 291, 734, 841, 870
AgsI TTSAA 6 cut(s) 163, 497, 686, 747, 829, 1010
AhlI ACTAGT 1 cut(s) 934
AjnI CCWGG 1 cut(s) 1062
AjuI GAANNNNNNNTTGG 2 cut(s) 341, 373
AluBI AGCT 5 cut(s) 17, 88, 129, 915, 1049
AluI AGCT 5 cut(s) 17, 88, 129, 915, 1049
Alw26I GTCTC 1 cut(s) 231
AlwI GGATC 2 cut(s) 665, 1034
Ama87I CYCGRG 1 cut(s) 56
Aor51HI AGCGCT 1 cut(s) 613
AoxI GGCC 7 cut(s) 297, 577, 621, 873, 958, 1022, 1060
ApeKI GCWGC 5 cut(s) 893, 907, 1049, 1052, 1055
ApoI RAATTY 3 cut(s) 434, 502, 830
Asp700I GAANNNNTTC 1 cut(s) 9
AspLEI GCGC 1 cut(s) 614
AspS9I GGNCC 1 cut(s) 873
AsuC2I CCSGG 3 cut(s) 57, 58, 837
AsuHPI GGTGA 2 cut(s) 490, 1043
AvaI CYCGRG 1 cut(s) 56
AxyI CCTNAGG 1 cut(s) 869
BaeGI GKGCMC 1 cut(s) 654
BanII GRGCYC 1 cut(s) 382
BbvI GCAGC 5 cut(s) 880, 919, 1036, 1064, 1067
BccI CCATC 4 cut(s) 78, 422, 586, 872
BcgI CGANNNNNNTGC 2 cut(s) 170, 204
BciT130I CCWGG 1 cut(s) 1064
BciVI GTATCC 1 cut(s) 281
BclI TGATCA 2 cut(s) 37, 1033
BcnI CCSGG 3 cut(s) 57, 58, 837
BcoDI GTCTC 1 cut(s) 231
BcuI ACTAGT 1 cut(s) 934
BfaI CTAG 1 cut(s) 935
BfmI CTRYAG 3 cut(s) 322, 607, 1050
BfoI RGCGCY 1 cut(s) 615
BfuAI ACCTGC 1 cut(s) 637
BfuI GTATCC 1 cut(s) 281
BglII AGATCT 1 cut(s) 318
BisI GCNGC 5 cut(s) 894, 908, 1050, 1053, 1056
BlsI GCNGC 5 cut(s) 895, 909, 1051, 1054, 1057
Bme1390I CCNGG 4 cut(s) 57, 58, 837, 1064
BmeT110I CYCGRG 1 cut(s) 56
BmgT120I GGNCC 1 cut(s) 873
BmrFI CCNGG 4 cut(s) 57, 58, 837, 1064
BmrI ACTGGG 1 cut(s) 412
BmuI ACTGGG 1 cut(s) 412
BpmI CTGGAG 1 cut(s) 1029
BpuMI CCSGG 3 cut(s) 57, 58, 837
Bsa29I ATCGAT 1 cut(s) 45
BsaAI YACGTR 1 cut(s) 649
BsaBI GATNNNNATC 1 cut(s) 1038
BsaJI CCNNGG 8 cut(s) 56, 144, 176, 188, 357, 618, 694, 835
Bsc4I CCNNNNNNNGG 4 cut(s) 291, 734, 841, 870
Bse118I RCCGGY 1 cut(s) 956
Bse1I ACTGG 3 cut(s) 259, 296, 407
Bse21I CCTNAGG 1 cut(s) 869
Bse8I GATNNNNATC 1 cut(s) 1038
BseBI CCWGG 1 cut(s) 1064
BseCI ATCGAT 1 cut(s) 45
BseDI CCNNGG 8 cut(s) 56, 144, 176, 188, 357, 618, 694, 835
BseGI GGATG 5 cut(s) 40, 70, 351, 433, 864
BseJI GATNNNNATC 1 cut(s) 1038
BseLI CCNNNNNNNGG 4 cut(s) 291, 734, 841, 870
BseMII CTCAG 3 cut(s) 251, 627, 860
BseNI ACTGG 3 cut(s) 259, 296, 407
BseRI GAGGAG 1 cut(s) 44
BseSI GKGCMC 1 cut(s) 654
BseXI GCAGC 5 cut(s) 880, 919, 1036, 1064, 1067
BseYI CCCAGC 1 cut(s) 17
Bsh1285I CGRYCG 1 cut(s) 279
BshFI GGCC 7 cut(s) 299, 579, 623, 875, 960, 1024, 1062
BshVI ATCGAT 1 cut(s) 45
BsiEI CGRYCG 1 cut(s) 279
BsiHKCI CYCGRG 1 cut(s) 56
BsiSI CCGG 4 cut(s) 57, 285, 837, 957
BslFI GGGAC 2 cut(s) 625, 931
BslI CCNNNNNNNGG 4 cut(s) 291, 734, 841, 870
BsmAI GTCTC 1 cut(s) 231
BsmFI GGGAC 2 cut(s) 625, 931
BsnI GGCC 7 cut(s) 299, 579, 623, 875, 960, 1024, 1062
BsoBI CYCGRG 1 cut(s) 56
Bsp1286I GDGCHC 2 cut(s) 382, 654
Bsp143I GATC 7 cut(s) 37, 42, 318, 670, 856, 1033, 1039
Bsp19I CCATGG 1 cut(s) 144
BspANI GGCC 7 cut(s) 299, 579, 623, 875, 960, 1024, 1062
BspCNI CTCAG 3 cut(s) 252, 628, 861
BspDI ATCGAT 1 cut(s) 45
BspMAI CTGCAG 1 cut(s) 1054
BspMI ACCTGC 1 cut(s) 637
BspPI GGATC 2 cut(s) 665, 1034
BspQI GCTCTTC 2 cut(s) 78, 370
BsrFI RCCGGY 1 cut(s) 956
BsrI ACTGG 3 cut(s) 259, 296, 407
BssAI RCCGGY 1 cut(s) 956
BssECI CCNNGG 8 cut(s) 56, 144, 176, 188, 357, 618, 694, 835
BssMI GATC 7 cut(s) 37, 42, 318, 670, 856, 1033, 1039
BssT1I CCWWGG 5 cut(s) 144, 176, 357, 618, 694
Bst2UI CCWGG 1 cut(s) 1064
Bst4CI ACNGT 1 cut(s) 326
Bst6I CTCTTC 4 cut(s) 78, 370, 757, 994
BstBAI YACGTR 1 cut(s) 649
BstC8I GCNNGC 1 cut(s) 1060
BstDEI CTNAG 3 cut(s) 260, 636, 869
BstDSI CCRYGG 1 cut(s) 144
BstF5I GGATG 5 cut(s) 40, 70, 351, 433, 864
BstH2I RGCGCY 1 cut(s) 615
BstHHI GCGC 1 cut(s) 614
BstKTI GATC 7 cut(s) 40, 45, 321, 673, 859, 1036, 1042
BstMAI GTCTC 1 cut(s) 231
BstMBI GATC 7 cut(s) 37, 42, 318, 670, 856, 1033, 1039
BstMCI CGRYCG 1 cut(s) 279
BstMWI GCNNNNNNNGC 6 cut(s) 281, 620, 629, 806, 966, 1055
BstNI CCWGG 1 cut(s) 1064
BstSCI CCNGG 4 cut(s) 55, 56, 835, 1062
BstSFI CTRYAG 3 cut(s) 322, 607, 1050
BstSLI GKGCMC 1 cut(s) 654
BstV1I GCAGC 5 cut(s) 880, 919, 1036, 1064, 1067
BstX2I RGATCY 2 cut(s) 318, 1039
BstXI CCANNNNNNTGG 1 cut(s) 530
BstYI RGATCY 2 cut(s) 318, 1039
Bsu15I ATCGAT 1 cut(s) 45
Bsu36I CCTNAGG 1 cut(s) 869
BsuI GTATCC 1 cut(s) 281
BsuRI GGCC 7 cut(s) 299, 579, 623, 875, 960, 1024, 1062
BsuTUI ATCGAT 1 cut(s) 45
BtgI CCRYGG 1 cut(s) 144
BtsCI GGATG 5 cut(s) 40, 70, 351, 433, 864
BtsIMutI CAGTG 1 cut(s) 675
BveI ACCTGC 1 cut(s) 637
Cac8I GCNNGC 1 cut(s) 1060
CfoI GCGC 1 cut(s) 614
Cfr10I RCCGGY 1 cut(s) 956
Cfr13I GGNCC 1 cut(s) 873
Cfr9I CCCGGG 1 cut(s) 56
ClaI ATCGAT 1 cut(s) 45
Csp6I GTAC 3 cut(s) 220, 247, 327
CviAII CATG 5 cut(s) 145, 413, 715, 901, 997
CviQI GTAC 3 cut(s) 220, 247, 327
DdeI CTNAG 3 cut(s) 260, 636, 869
DpnI GATC 7 cut(s) 39, 44, 320, 672, 858, 1035, 1041
DpnII GATC 7 cut(s) 37, 42, 318, 670, 856, 1033, 1039
Eam1104I CTCTTC 4 cut(s) 78, 370, 757, 994
EarI CTCTTC 4 cut(s) 78, 370, 757, 994
Eco130I CCWWGG 5 cut(s) 144, 176, 357, 618, 694
Eco147I AGGCCT 1 cut(s) 1062
Eco24I GRGCYC 1 cut(s) 382
Eco47III AGCGCT 1 cut(s) 613
Eco57I CTGAAG 1 cut(s) 975
Eco81I CCTNAGG 1 cut(s) 869
Eco88I CYCGRG 1 cut(s) 56
EcoO109I RGGNCCY 1 cut(s) 873
EcoRI GAATTC 1 cut(s) 502
EcoRII CCWGG 1 cut(s) 1062
EcoT14I CCWWGG 5 cut(s) 144, 176, 357, 618, 694
EcoT22I ATGCAT 1 cut(s) 414
EcoT38I GRGCYC 1 cut(s) 382
ErhI CCWWGG 5 cut(s) 144, 176, 357, 618, 694
FaeI CATG 5 cut(s) 148, 416, 718, 904, 1000
FalI AAGNNNNNCTT 2 cut(s) 341, 373
FaqI GGGAC 2 cut(s) 625, 931
FatI CATG 5 cut(s) 144, 412, 714, 900, 996
FauNDI CATATG 1 cut(s) 1027
FbaI TGATCA 2 cut(s) 37, 1033
Fnu4HI GCNGC 5 cut(s) 894, 908, 1050, 1053, 1056
FokI GGATG 5 cut(s) 47, 57, 358, 440, 851
FriOI GRGCYC 1 cut(s) 382
Fsp4HI GCNGC 5 cut(s) 894, 908, 1050, 1053, 1056
FspBI CTAG 1 cut(s) 935
GlaI GCGC 1 cut(s) 613
GluI GCNGC 5 cut(s) 894, 908, 1050, 1053, 1056
GsaI CCCAGC 1 cut(s) 21
GsuI CTGGAG 1 cut(s) 1029
HaeII RGCGCY 1 cut(s) 615
HaeIII GGCC 7 cut(s) 299, 579, 623, 875, 960, 1024, 1062
HapII CCGG 4 cut(s) 57, 285, 837, 957
HhaI GCGC 1 cut(s) 614
Hin1II CATG 5 cut(s) 148, 416, 718, 904, 1000
Hin6I GCGC 1 cut(s) 612
HinP1I GCGC 1 cut(s) 612
HinfI GANTC 3 cut(s) 596, 638, 771
HpaII CCGG 4 cut(s) 57, 285, 837, 957
HphI GGTGA 2 cut(s) 490, 1043
Hpy166II GTNNAC 2 cut(s) 157, 445
Hpy188I TCNGA 7 cut(s) 42, 172, 192, 261, 557, 637, 844
Hpy188III TCNNGA 4 cut(s) 531, 868, 1007, 1037
Hpy8I GTNNAC 2 cut(s) 157, 445
HpyAV CCTTC 3 cut(s) 106, 289, 825
HpyCH4III ACNGT 1 cut(s) 326
HpyCH4IV ACGT 2 cut(s) 9, 648
HpyCH4V TGCA 7 cut(s) 106, 412, 464, 486, 853, 896, 1052
HpyF10VI GCNNNNNNNGC 6 cut(s) 281, 620, 629, 806, 966, 1055
HpyF3I CTNAG 3 cut(s) 260, 636, 869
HpySE526I ACGT 2 cut(s) 9, 648
Hsp92II CATG 5 cut(s) 148, 416, 718, 904, 1000
HspAI GCGC 1 cut(s) 612
Ksp22I TGATCA 2 cut(s) 37, 1033
Kzo9I GATC 7 cut(s) 37, 42, 318, 670, 856, 1033, 1039
LguI GCTCTTC 2 cut(s) 78, 370
LmnI GCTCC 2 cut(s) 619, 814
Lsp1109I GCAGC 5 cut(s) 880, 919, 1036, 1064, 1067
MaeI CTAG 1 cut(s) 935
MaeII ACGT 2 cut(s) 9, 648
MaeIII GTNAC 2 cut(s) 398, 538
MalI GATC 7 cut(s) 39, 44, 320, 672, 858, 1035, 1041
MboI GATC 7 cut(s) 37, 42, 318, 670, 856, 1033, 1039
MboII GAAGA 8 cut(s) 95, 361, 387, 564, 591, 735, 744, 981
MfeI CAATTG 1 cut(s) 182
MflI RGATCY 2 cut(s) 318, 1039
MhlI GDGCHC 2 cut(s) 382, 654
MluCI AATT 8 cut(s) 182, 434, 502, 564, 784, 830, 1069, 1078
MlyI GAGTC 1 cut(s) 647
MmeI TCCRAC 3 cut(s) 98, 687, 780
Mph1103I ATGCAT 1 cut(s) 414
MroXI GAANNNNTTC 1 cut(s) 9
MseI TTAA 1 cut(s) 1084
MspA1I CMGCKG 2 cut(s) 17, 1049
MspI CCGG 4 cut(s) 57, 285, 837, 957
MspR9I CCNGG 4 cut(s) 57, 58, 837, 1064
MunI CAATTG 1 cut(s) 182
MvaI CCWGG 1 cut(s) 1064
MwoI GCNNNNNNNGC 6 cut(s) 281, 620, 629, 806, 966, 1055
NciI CCSGG 3 cut(s) 57, 58, 837
NcoI CCATGG 1 cut(s) 144
NdeI CATATG 1 cut(s) 1027
NdeII GATC 7 cut(s) 37, 42, 318, 670, 856, 1033, 1039
NlaIII CATG 5 cut(s) 148, 416, 718, 904, 1000
NmuCI GTSAC 1 cut(s) 538
NsiI ATGCAT 1 cut(s) 414
PceI AGGCCT 1 cut(s) 1062
PciSI GCTCTTC 2 cut(s) 78, 370
PdmI GAANNNNTTC 1 cut(s) 9
PfeI GAWTC 2 cut(s) 596, 771
PkrI GCNGC 5 cut(s) 895, 909, 1051, 1054, 1057
PleI GAGTC 1 cut(s) 646
PpsI GAGTC 1 cut(s) 646
Ppu21I YACGTR 1 cut(s) 649
Psp1406I AACGTT 1 cut(s) 9
Psp6I CCWGG 1 cut(s) 1062
PspFI CCCAGC 1 cut(s) 17
PspGI CCWGG 1 cut(s) 1062
PspPI GGNCC 1 cut(s) 873
PstI CTGCAG 1 cut(s) 1054
PsuI RGATCY 2 cut(s) 318, 1039
PvuII CAGCTG 2 cut(s) 17, 1049
RsaI GTAC 3 cut(s) 221, 248, 328
RsaNI GTAC 3 cut(s) 220, 247, 327
SapI GCTCTTC 2 cut(s) 78, 370
SaqAI TTAA 1 cut(s) 1084
SatI GCNGC 5 cut(s) 894, 908, 1050, 1053, 1056
Sau3AI GATC 7 cut(s) 37, 42, 318, 670, 856, 1033, 1039
Sau96I GGNCC 1 cut(s) 873
SchI GAGTC 1 cut(s) 647
ScrFI CCNGG 4 cut(s) 57, 58, 837, 1064
SduI GDGCHC 2 cut(s) 382, 654
SfcI CTRYAG 3 cut(s) 322, 607, 1050
SmaI CCCGGG 1 cut(s) 58
SpeI ACTAGT 1 cut(s) 934
Sse9I AATT 8 cut(s) 182, 434, 502, 564, 784, 830, 1069, 1078
SseBI AGGCCT 1 cut(s) 1062
SspI AATATT 1 cut(s) 703
SspMI CTAG 1 cut(s) 935
StuI AGGCCT 1 cut(s) 1062
StyD4I CCNGG 4 cut(s) 55, 56, 835, 1062
StyI CCWWGG 5 cut(s) 144, 176, 357, 618, 694
TaaI ACNGT 1 cut(s) 326
TaiI ACGT 2 cut(s) 12, 651
TaqI TCGA 2 cut(s) 45, 818
TaqII GACCGA 1 cut(s) 293
TasI AATT 8 cut(s) 182, 434, 502, 564, 784, 830, 1069, 1078
TatI WGTACW 1 cut(s) 326
TfiI GAWTC 2 cut(s) 596, 771
Tru1I TTAA 1 cut(s) 1084
Tru9I TTAA 1 cut(s) 1084
TscAI CASTG 1 cut(s) 682
TseFI GTSAC 1 cut(s) 538
TseI GCWGC 5 cut(s) 893, 907, 1049, 1052, 1055
Tsp45I GTSAC 1 cut(s) 538
TspDTI ATGAA 8 cut(s) 96, 362, 429, 486, 576, 731, 777, 989
TspMI CCCGGG 1 cut(s) 56
TspRI CASTG 1 cut(s) 682
XapI RAATTY 3 cut(s) 434, 502, 830
XcmI CCANNNNNNNNNTGG 2 cut(s) 151, 1009
XmaI CCCGGG 1 cut(s) 56
XmnI GAANNNNTTC 1 cut(s) 9
XspI CTAG 1 cut(s) 935
Zsp2I ATGCAT 1 cut(s) 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.