MD06G1220700.v1.1

RING-H2 zinc finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0007225
Physical Location & Seq
Forward (+)
35253933 .. 35256802
2870 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1220700.v1.1.491

Sequence Viewer

Length: 1146 bp
ATGCCTCTCACAGAGAGCTCAACTGCAGGAGCAGAGCACATGAAATGGAGAAGACCCAGAACCCAATTCAACCAACCCATTTCAGAAACGGATCCAGCCACCTCCCCCCTCCAATCCACCCGCTGCAAATCCACCATCTCCTCCCTCCTCCTCTCCAGCTTCACTACCTCCACCACCAACAACAACGAAGCCTTACCCACAAGCCTTACCATCAAGAAAAAGACAAACTTTCCCTCGTCGTCGTTCAGAGGCCTGGGCTGCGCCGCCTCCGCCTCGCAGCAAGTCTCGGTGCCGGAGGAGATTCGGAACTCCGCCGATTGGAAGGGAAAGAAGGTGAAGAAGAAAAAGGAGAAGAAGAGCGCCGCCGCCAAGGGCAGCGCTAGCAACGAGAAGAATGAATACCGGGATGTCGGTGATGGCGGGCCCACCATTGGTATAAATTCTGCAACTTGTGTGGATTTTCAGGATGTTTGGTGCGGCCCCGGAATTGGGTTCTCGCCGGAAACTGCGGGCTCGGTGGATTGCGTGGTGGCGAGGAGGAATGTCTCCGGCAGAGGCAAGATTTATGGGGATAATAATAAGATGAGCAGCCACATGAGCAGCCACAGGGAGCGTCCTTGTTTCGCAAGGCGAAACGTGAGCCCCGAAACCATCTCGTTTCTGGATTCTGAACCGGATTTTGTCTCGAGCCGCCCTGAATCAGAGGTATTTGGAAGTAGGTGTTTTCGGCATGTCCGGCATCCATCCCCTGAAGGCCTTGCAGAGATTATGCTGTTTCAAAGTAGTCTTATGATGGGTGGAAGAATGGATAGATTCCGTGACTGGAGACTCGATGTTGATAGCATGTCATATGAGCAACTGCTCGAGTTAGGCGAGAGAATTGGCAACGTGAGTACTGGATTAAAAGAAGATGAAATAAACCACTGCCTTCGAAAGATTAAGCTTCCAACGTCGAGTGATGCGACATCACACTCATCTGCGAAAGTAGAAAGCAAATGCATCATTTGTCAGGAGGAGTACGAAGCAGATGACGAGATGGGCAAACTGCACTGCGGACATAACTTCCACTTACAATGTGTAAAACAGTGGCTCACACAAAAGAATACGTGCCCTTTCTGCAAGGTCGAAGCAATATCTAGATGCTAG

Protein Analysis

382

Amino Acids

42.2

Weight (kDa)

8.56

Isoelectric Point (pI)

48.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 327 - 374 1.1e-10 RING-H2 zinc finger domain
zf-RING_2 PF13639 332 - 374 7.3e-14 Ring finger domain
zf-C3HC4_2 PF13923 332 - 373 8.2e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 333 - 371 4.6e-07 RING-type zinc-finger
zf-C3HC4 PF00097 333 - 373 1.6e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 289
AclWI GGATC 2 cut(s) 86, 99
AcsI RAATTY 1 cut(s) 439
AcuI CTGAAG 1 cut(s) 771
AfaI GTAC 2 cut(s) 895, 1019
AfeI AGCGCT 1 cut(s) 379
AfiI CCNNNNNNNGG 4 cut(s) 318, 431, 488, 489
AgsI TTSAA 2 cut(s) 70, 779
AjnI CCWGG 1 cut(s) 252
AluBI AGCT 3 cut(s) 18, 159, 943
AluI AGCT 3 cut(s) 18, 159, 943
Alw21I GWGCWC 2 cut(s) 20, 39
Alw26I GTCTC 4 cut(s) 289, 550, 688, 820
AlwI GGATC 2 cut(s) 86, 99
Ama87I CYCGRG 2 cut(s) 685, 863
Aor51HI AGCGCT 1 cut(s) 379
AoxI GGCC 4 cut(s) 250, 422, 478, 754
ApaI GGGCCC 1 cut(s) 426
ApeKI GCWGC 6 cut(s) 123, 258, 277, 375, 588, 600
ApoI RAATTY 1 cut(s) 439
AspLEI GCGC 3 cut(s) 263, 362, 380
AspS9I GGNCC 3 cut(s) 422, 423, 479
AsuC2I CCSGG 2 cut(s) 404, 483
AsuHPI GGTGA 2 cut(s) 346, 425
AsuII TTCGAA 1 cut(s) 931
AsuNHI GCTAGC 1 cut(s) 380
AvaI CYCGRG 2 cut(s) 685, 863
BaeGI GKGCMC 2 cut(s) 426, 1112
BamHI GGATCC 1 cut(s) 91
BanI GGYRCC 1 cut(s) 289
BanII GRGCYC 4 cut(s) 20, 426, 515, 644
BarI GAAGNNNNNNTAC 2 cut(s) 383, 415
BbsI GAAGAC 1 cut(s) 58
Bbv12I GWGCWC 2 cut(s) 20, 39
BbvI GCAGC 6 cut(s) 110, 245, 289, 387, 600, 612
BccI CCATC 7 cut(s) 143, 218, 410, 659, 751, 787, 1030
BciT130I CCWGG 1 cut(s) 254
BcnI CCSGG 2 cut(s) 404, 483
BcoDI GTCTC 4 cut(s) 289, 550, 688, 820
BfaI CTAG 3 cut(s) 381, 1137, 1144
BfmI CTRYAG 1 cut(s) 24
BfoI RGCGCY 2 cut(s) 363, 381
BmcAI AGTACT 1 cut(s) 895
Bme1390I CCNGG 3 cut(s) 254, 404, 483
BmeT110I CYCGRG 2 cut(s) 685, 863
BmgT120I GGNCC 3 cut(s) 422, 423, 479
BmiI GGNNCC 4 cut(s) 93, 291, 424, 481
BmrFI CCNGG 3 cut(s) 254, 404, 483
BmsI GCATC 4 cut(s) 748, 949, 1008, 1130
BmtI GCTAGC 1 cut(s) 384
BpiI GAAGAC 1 cut(s) 58
BpmI CTGGAG 2 cut(s) 139, 844
Bpu14I TTCGAA 1 cut(s) 931
BpuMI CCSGG 2 cut(s) 404, 483
BsaAI YACGTR 1 cut(s) 1107
BsaJI CCNNGG 3 cut(s) 253, 369, 481
BsaWI WCCGGW 1 cut(s) 673
Bsc4I CCNNNNNNNGG 4 cut(s) 318, 431, 488, 489
Bse1I ACTGG 2 cut(s) 827, 901
BseBI CCWGG 1 cut(s) 254
BseDI CCNNGG 3 cut(s) 253, 369, 481
BseGI GGATG 4 cut(s) 412, 472, 739, 743
BseLI CCNNNNNNNGG 4 cut(s) 318, 431, 488, 489
BseNI ACTGG 2 cut(s) 827, 901
BseRI GAGGAG 6 cut(s) 130, 137, 140, 311, 550, 1028
BseSI GKGCMC 2 cut(s) 426, 1112
BseXI GCAGC 6 cut(s) 110, 245, 289, 387, 600, 612
BsgI GTGCAG 1 cut(s) 1031
BshFI GGCC 4 cut(s) 252, 424, 480, 756
BshNI GGYRCC 1 cut(s) 289
BsiHKAI GWGCWC 2 cut(s) 20, 39
BsiHKCI CYCGRG 2 cut(s) 685, 863
BsiSI CCGG 7 cut(s) 293, 403, 483, 500, 549, 674, 736
BslI CCNNNNNNNGG 4 cut(s) 318, 431, 488, 489
BsmAI GTCTC 4 cut(s) 289, 550, 688, 820
BsnI GGCC 4 cut(s) 252, 424, 480, 756
BsoBI CYCGRG 2 cut(s) 685, 863
Bsp119I TTCGAA 1 cut(s) 931
Bsp120I GGGCCC 1 cut(s) 422
Bsp1286I GDGCHC 6 cut(s) 20, 39, 426, 515, 644, 1112
Bsp143I GATC 1 cut(s) 91
BspANI GGCC 4 cut(s) 252, 424, 480, 756
BspLI GGNNCC 4 cut(s) 93, 291, 424, 481
BspMAI CTGCAG 1 cut(s) 28
BspOI GCTAGC 1 cut(s) 384
BspPI GGATC 2 cut(s) 86, 99
BspQI GCTCTTC 1 cut(s) 350
BspT104I TTCGAA 1 cut(s) 931
BspT107I GGYRCC 1 cut(s) 289
BsrI ACTGG 2 cut(s) 827, 901
BssECI CCNNGG 3 cut(s) 253, 369, 481
BssMI GATC 1 cut(s) 91
BssT1I CCWWGG 1 cut(s) 369
Bst2UI CCWGG 1 cut(s) 254
Bst4CI ACNGT 1 cut(s) 1086
Bst6I CTCTTC 1 cut(s) 350
BstBAI YACGTR 1 cut(s) 1107
BstBI TTCGAA 1 cut(s) 931
BstC8I GCNNGC 3 cut(s) 382, 422, 511
BstF5I GGATG 4 cut(s) 412, 472, 739, 743
BstH2I RGCGCY 2 cut(s) 363, 381
BstHHI GCGC 3 cut(s) 263, 362, 380
BstKTI GATC 1 cut(s) 94
BstMAI GTCTC 4 cut(s) 289, 550, 688, 820
BstMBI GATC 1 cut(s) 91
BstMWI GCNNNNNNNGC 6 cut(s) 258, 269, 381, 597, 736, 1116
BstNI CCWGG 1 cut(s) 254
BstNSI RCATGY 2 cut(s) 734, 847
BstSCI CCNGG 3 cut(s) 252, 402, 481
BstSFI CTRYAG 1 cut(s) 24
BstSLI GKGCMC 2 cut(s) 426, 1112
BstV1I GCAGC 6 cut(s) 110, 245, 289, 387, 600, 612
BstV2I GAAGAC 1 cut(s) 58
BstX2I RGATCY 1 cut(s) 91
BstYI RGATCY 1 cut(s) 91
BsuRI GGCC 4 cut(s) 252, 424, 480, 756
BtsCI GGATG 4 cut(s) 412, 472, 739, 743
BtsI GCAGTG 2 cut(s) 922, 1048
BtsIMutI CAGTG 3 cut(s) 922, 1048, 1091
Cac8I GCNNGC 3 cut(s) 382, 422, 511
CfoI GCGC 3 cut(s) 263, 362, 380
Cfr13I GGNCC 3 cut(s) 422, 423, 479
CseI GACGC 1 cut(s) 602
Csp6I GTAC 2 cut(s) 894, 1018
CspCI CAANNNNNGTGG 2 cut(s) 435, 470
CviAII CATG 4 cut(s) 40, 595, 731, 844
CviQI GTAC 2 cut(s) 894, 1018
DpnI GATC 1 cut(s) 93
DpnII GATC 1 cut(s) 91
Eam1104I CTCTTC 1 cut(s) 350
EarI CTCTTC 1 cut(s) 350
EciI GGCGGA 2 cut(s) 259, 301
Ecl136II GAGCTC 1 cut(s) 18
Eco130I CCWWGG 1 cut(s) 369
Eco147I AGGCCT 2 cut(s) 252, 756
Eco24I GRGCYC 4 cut(s) 20, 426, 515, 644
Eco47III AGCGCT 1 cut(s) 379
Eco53kI GAGCTC 1 cut(s) 18
Eco57I CTGAAG 1 cut(s) 771
Eco88I CYCGRG 2 cut(s) 685, 863
EcoICRI GAGCTC 1 cut(s) 18
EcoRII CCWGG 1 cut(s) 252
EcoT14I CCWWGG 1 cut(s) 369
EcoT22I ATGCAT 1 cut(s) 1001
EcoT38I GRGCYC 4 cut(s) 20, 426, 515, 644
ErhI CCWWGG 1 cut(s) 369
FaeI CATG 4 cut(s) 43, 598, 734, 847
FalI AAGNNNNNCTT 2 cut(s) 212, 244
FatI CATG 4 cut(s) 39, 594, 730, 843
FauI CCCGC 3 cut(s) 128, 413, 502
FauNDI CATATG 1 cut(s) 850
FokI GGATG 4 cut(s) 419, 479, 726, 730
FriOI GRGCYC 4 cut(s) 20, 426, 515, 644
FspBI CTAG 3 cut(s) 381, 1137, 1144
GlaI GCGC 3 cut(s) 262, 361, 379
GsuI CTGGAG 2 cut(s) 139, 844
HaeII RGCGCY 2 cut(s) 363, 381
HaeIII GGCC 4 cut(s) 252, 424, 480, 756
HapII CCGG 7 cut(s) 293, 403, 483, 500, 549, 674, 736
HgaI GACGC 1 cut(s) 602
HhaI GCGC 3 cut(s) 263, 362, 380
Hin1II CATG 4 cut(s) 43, 598, 734, 847
Hin6I GCGC 3 cut(s) 261, 360, 378
HinP1I GCGC 3 cut(s) 261, 360, 378
HindIII AAGCTT 1 cut(s) 941
HinfI GANTC 5 cut(s) 301, 665, 698, 813, 828
HpaII CCGG 7 cut(s) 293, 403, 483, 500, 549, 674, 736
HphI GGTGA 2 cut(s) 346, 425
Hpy188I TCNGA 5 cut(s) 85, 248, 306, 670, 703
Hpy188III TCNNGA 6 cut(s) 214, 464, 662, 685, 1010, 1137
Hpy99I CGWCG 3 cut(s) 241, 244, 955
HpyAV CCTTC 4 cut(s) 316, 325, 746, 938
HpyCH4III ACNGT 1 cut(s) 1086
HpyCH4IV ACGT 4 cut(s) 636, 888, 950, 1106
HpyCH4V TGCA 7 cut(s) 26, 126, 446, 761, 999, 1048, 1119
HpyF10VI GCNNNNNNNGC 6 cut(s) 258, 269, 381, 597, 736, 1116
HpySE526I ACGT 4 cut(s) 636, 888, 950, 1106
Hsp92II CATG 4 cut(s) 43, 598, 734, 847
HspAI GCGC 3 cut(s) 261, 360, 378
Kzo9I GATC 1 cut(s) 91
LguI GCTCTTC 1 cut(s) 350
LmnI GCTCC 2 cut(s) 29, 610
Lsp1109I GCAGC 6 cut(s) 110, 245, 289, 387, 600, 612
LweI GCATC 4 cut(s) 748, 949, 1008, 1130
MaeI CTAG 3 cut(s) 381, 1137, 1144
MaeII ACGT 4 cut(s) 636, 888, 950, 1106
MaeIII GTNAC 1 cut(s) 818
MalI GATC 1 cut(s) 93
MboI GATC 1 cut(s) 91
MboII GAAGA 8 cut(s) 63, 349, 352, 364, 367, 403, 813, 920
MflI RGATCY 1 cut(s) 91
MhlI GDGCHC 6 cut(s) 20, 39, 426, 515, 644, 1112
MluCI AATT 4 cut(s) 65, 439, 486, 879
MlyI GAGTC 1 cut(s) 822
MmeI TCCRAC 1 cut(s) 971
Mph1103I ATGCAT 1 cut(s) 1001
MseI TTAA 2 cut(s) 902, 939
MspA1I CMGCKG 1 cut(s) 123
MspI CCGG 7 cut(s) 293, 403, 483, 500, 549, 674, 736
MspR9I CCNGG 3 cut(s) 254, 404, 483
MvaI CCWGG 1 cut(s) 254
MwoI GCNNNNNNNGC 6 cut(s) 258, 269, 381, 597, 736, 1116
NciI CCSGG 2 cut(s) 404, 483
NdeI CATATG 1 cut(s) 850
NdeII GATC 1 cut(s) 91
NheI GCTAGC 1 cut(s) 380
NlaIII CATG 4 cut(s) 43, 598, 734, 847
NlaIV GGNNCC 4 cut(s) 93, 291, 424, 481
NmuCI GTSAC 1 cut(s) 818
NsiI ATGCAT 1 cut(s) 1001
NspI RCATGY 2 cut(s) 734, 847
NspV TTCGAA 1 cut(s) 931
PaeR7I CTCGAG 2 cut(s) 685, 863
PceI AGGCCT 2 cut(s) 252, 756
PciSI GCTCTTC 1 cut(s) 350
PcsI WCGNNNNNNNCGW 3 cut(s) 642, 870, 959
PfeI GAWTC 4 cut(s) 301, 665, 698, 813
PleI GAGTC 1 cut(s) 822
PpsI GAGTC 1 cut(s) 822
Ppu21I YACGTR 1 cut(s) 1107
Psp124BI GAGCTC 1 cut(s) 20
Psp6I CCWGG 1 cut(s) 252
PspGI CCWGG 1 cut(s) 252
PspN4I GGNNCC 4 cut(s) 93, 291, 424, 481
PspOMI GGGCCC 1 cut(s) 422
PspPI GGNCC 3 cut(s) 422, 423, 479
PspXI VCTCGAGB 1 cut(s) 863
PstI CTGCAG 1 cut(s) 28
PsuI RGATCY 1 cut(s) 91
RsaI GTAC 2 cut(s) 895, 1019
RsaNI GTAC 2 cut(s) 894, 1018
SacI GAGCTC 1 cut(s) 20
SapI GCTCTTC 1 cut(s) 350
SaqAI TTAA 2 cut(s) 902, 939
Sau3AI GATC 1 cut(s) 91
Sau96I GGNCC 3 cut(s) 422, 423, 479
ScaI AGTACT 1 cut(s) 895
SchI GAGTC 1 cut(s) 822
ScrFI CCNGG 3 cut(s) 254, 404, 483
SduI GDGCHC 6 cut(s) 20, 39, 426, 515, 644, 1112
SfaNI GCATC 4 cut(s) 748, 949, 1008, 1130
SfcI CTRYAG 1 cut(s) 24
Sfr274I CTCGAG 2 cut(s) 685, 863
SfuI TTCGAA 1 cut(s) 931
SlaI CTCGAG 2 cut(s) 685, 863
SmlI CTYRAG 2 cut(s) 685, 863
SmoI CTYRAG 2 cut(s) 685, 863
Sse9I AATT 4 cut(s) 65, 439, 486, 879
SseBI AGGCCT 2 cut(s) 252, 756
SspMI CTAG 3 cut(s) 381, 1137, 1144
SstI GAGCTC 1 cut(s) 20
StuI AGGCCT 2 cut(s) 252, 756
StyD4I CCNGG 3 cut(s) 252, 402, 481
StyI CCWWGG 1 cut(s) 369
TaaI ACNGT 1 cut(s) 1086
TaiI ACGT 4 cut(s) 639, 891, 953, 1109
TaqI TCGA 6 cut(s) 686, 831, 864, 931, 953, 1125
TasI AATT 4 cut(s) 65, 439, 486, 879
TatI WGTACW 1 cut(s) 893
TauI GCSGC 5 cut(s) 266, 365, 368, 480, 693
TfiI GAWTC 4 cut(s) 301, 665, 698, 813
Tru1I TTAA 2 cut(s) 902, 939
Tru9I TTAA 2 cut(s) 902, 939
TscAI CASTG 3 cut(s) 929, 1055, 1091
TseFI GTSAC 1 cut(s) 818
TseI GCWGC 6 cut(s) 123, 258, 277, 375, 588, 600
Tsp45I GTSAC 1 cut(s) 818
TspDTI ATGAA 3 cut(s) 56, 411, 927
TspGWI ACGGA 2 cut(s) 104, 806
TspRI CASTG 3 cut(s) 929, 1055, 1091
XapI RAATTY 1 cut(s) 439
XbaI TCTAGA 1 cut(s) 1136
XceI RCATGY 2 cut(s) 734, 847
XcmI CCANNNNNNNNNTGG 1 cut(s) 658
XhoI CTCGAG 2 cut(s) 685, 863
XspI CTAG 3 cut(s) 381, 1137, 1144
ZrmI AGTACT 1 cut(s) 895
Zsp2I ATGCAT 1 cut(s) 1001
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.