RchiOBHm_Chr7g0179401

RING-H2 zinc finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0007225
Physical Location & Seq
Reverse (-)
1683435 .. 1686406
2972 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ15992

Sequence Viewer

Length: 1134 bp
ATGCCTGTTCTTACAGAGAGCTCAGAGCACATGAAATGGAGAAGACCCAGAAGCCAATTAATCCAGCCCATTTCAGAAACAGATCCATACAACCCAAATCCAGCACCATCCATAATCCAATCCACTCGCTGCAAATCCACAATCTCCTCCCTCCTCCTCTCCAACTTCACCGCCACCACCAATGAAGCCTCATCCTCAAGCCTCAGCACCAAGAAGAAGACAAACTTCCCCTCATCAGCAAAGTTCAGAGGCTTGGGCTGCACAGCCTCAGCCTCACAGCAAGTCTCAGTGCCAGCTGTGATCAGGAGCTCAGCAGATTGGGAAGGGAAGAAGGTGAAGAAGAAAAAGGTTAATAAGAAGAATGTCAGGAATGAGTTCAAGGACAAGAGCCAGAACCAAGGAGTGGTTGATGGGCCTGTTGGGTTTGGGTTAAATTCTGCAACTTGTATGGATTTTCAGGATGTTTGGTGTGGGCCTGGAATTGGGTTCTCAGCAGAGACTGCTGGCTCTGTGGATTGTGTTGTGGCTAGGAGAAATGTTTCTGGAAGAGGCAAGATTGATGGGGATCATAATAGGATGAACAGCCACAGGGAGCGTCCTTGTTTAGCAAGGCGAACTGTGAACCCGGATACTATCTCGTTTCTTGATTCTGAACCTGAATTCCTCTTAAGCCGCCCTGGATCAGAGGTGTTTGGAAGTAGGTGTTATCGACATGTTCGCCATGCCTCTCCTGAAGGCCTCGCAGAGATTATGATGTTTCAAAGTAGTCTTATGATGGGTGGAAGATCAGACATGGATAGATATAGAGAGTGGAGACTTGATGTTGATAACATGACATATGAGGAACTGCTTGAGCTAGGTGAGAGAATTGGTTATGTGAGTACTGGATTAAAAGAAGATGAGATAAGCCGGTGCCTTCGAAAAATTAAGCTTTCAATGTTGAGTGATCGGTCACAACATTCACCTGGGCAAATAGATGGCAAGTGCATCATTTGTCAGGAGGAATATGAAGCAGATGATGAGATGGGTAGACTGAATTGTGGACACCTCTTCCACTTACAGTGTATAGAACAGTGGCTTGCTCATAAAAATACTTGCCCTTTTTGTAAGGTTGAAGCGACAACTAAACGCTAG

Protein Analysis

377

Amino Acids

42.22

Weight (kDa)

8.69

Isoelectric Point (pI)

47.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 324 - 370 1.1e-10 RING-H2 zinc finger domain
zf-RING_2 PF13639 328 - 370 7.9e-15 Ring finger domain
zf-C3HC4_2 PF13923 328 - 369 3.3e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 328 - 370 3.7e-06 zinc-RING finger domain
zf-C3HC4 PF00097 329 - 369 2.1e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 329 - 367 5.5e-07 RING-type zinc-finger
zf-RING_11 PF17123 329 - 356 2.5e-06 RING-like zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 912
AccB7I CCANNNNNTGG 1 cut(s) 403
AccI GTMKAC 1 cut(s) 1030
AciI CCGC 2 cut(s) 171, 673
AclWI GGATC 3 cut(s) 77, 573, 688
AcsI RAATTY 2 cut(s) 433, 659
AcuI CTGAAG 1 cut(s) 753
AfaI GTAC 1 cut(s) 883
AfiI CCNNNNNNNGG 2 cut(s) 403, 482
AflII CTTAAG 1 cut(s) 667
AflIII ACRYGT 1 cut(s) 712
AgsI TTSAA 4 cut(s) 379, 761, 936, 1115
AjnI CCWGG 3 cut(s) 475, 676, 964
AloI GAACNNNNNNTCC 2 cut(s) 645, 677
AluBI AGCT 5 cut(s) 21, 296, 309, 856, 931
AluI AGCT 5 cut(s) 21, 296, 309, 856, 931
Alw21I GWGCWC 3 cut(s) 23, 30, 311
Alw26I GTCTC 3 cut(s) 289, 491, 808
AlwI GGATC 3 cut(s) 77, 573, 688
AlwNI CAGNNNCTG 1 cut(s) 500
AoxI GGCC 3 cut(s) 413, 473, 736
ApeKI GCWGC 2 cut(s) 129, 258
ApoI RAATTY 2 cut(s) 433, 659
AseI ATTAAT 1 cut(s) 59
Asp700I GAANNNNTTC 2 cut(s) 374, 538
AspS9I GGNCC 2 cut(s) 413, 473
AsuC2I CCSGG 1 cut(s) 626
AsuHPI GGTGA 4 cut(s) 160, 346, 872, 954
AsuII TTCGAA 1 cut(s) 919
BanI GGYRCC 1 cut(s) 912
BanII GRGCYC 2 cut(s) 23, 311
BbsI GAAGAC 2 cut(s) 49, 224
Bbv12I GWGCWC 3 cut(s) 23, 30, 311
BbvCI CCTCAGC 2 cut(s) 203, 268
BbvI GCAGC 2 cut(s) 116, 245
BccI CCATC 6 cut(s) 115, 404, 554, 769, 971, 1018
BciT130I CCWGG 3 cut(s) 477, 678, 966
BciVI GTATCC 1 cut(s) 622
BclI TGATCA 1 cut(s) 300
BcnI CCSGG 1 cut(s) 626
BcoDI GTCTC 3 cut(s) 289, 491, 808
BfaI CTAG 3 cut(s) 528, 857, 1132
BfrI CTTAAG 1 cut(s) 667
BfuI GTATCC 1 cut(s) 622
BisI GCNGC 3 cut(s) 130, 259, 673
BlpI GCTNAGC 1 cut(s) 310
BlsI GCNGC 3 cut(s) 131, 260, 674
BmcAI AGTACT 1 cut(s) 883
Bme1390I CCNGG 4 cut(s) 477, 626, 678, 966
BmgT120I GGNCC 2 cut(s) 413, 473
BmiI GGNNCC 1 cut(s) 914
BmrFI CCNGG 4 cut(s) 477, 626, 678, 966
BmsI GCATC 1 cut(s) 996
BpiI GAAGAC 2 cut(s) 49, 224
Bpu10I CCTNAGC 2 cut(s) 203, 268
Bpu1102I GCTNAGC 1 cut(s) 310
Bpu14I TTCGAA 1 cut(s) 919
BpuEI CTTGAG 2 cut(s) 181, 872
BpuMI CCSGG 1 cut(s) 626
BsaBI GATNNNNATC 1 cut(s) 564
BsaJI CCNNGG 3 cut(s) 397, 676, 965
Bsc4I CCNNNNNNNGG 2 cut(s) 403, 482
Bse118I RCCGGY 1 cut(s) 909
Bse1I ACTGG 1 cut(s) 889
Bse8I GATNNNNATC 1 cut(s) 564
BseBI CCWGG 3 cut(s) 477, 678, 966
BseDI CCNNGG 3 cut(s) 397, 676, 965
BseGI GGATG 4 cut(s) 107, 191, 466, 582
BseJI GATNNNNATC 1 cut(s) 564
BseLI CCNNNNNNNGG 2 cut(s) 403, 482
BseMII CTCAG 6 cut(s) 36, 217, 282, 300, 324, 504
BseNI ACTGG 1 cut(s) 889
BseRI GAGGAG 3 cut(s) 136, 143, 146
BseXI GCAGC 2 cut(s) 116, 245
BsgI GTGCAG 1 cut(s) 244
BshFI GGCC 3 cut(s) 415, 475, 738
BshNI GGYRCC 1 cut(s) 912
BsiHKAI GWGCWC 3 cut(s) 23, 30, 311
BsiSI CCGG 2 cut(s) 626, 910
BslI CCNNNNNNNGG 2 cut(s) 403, 482
BsmAI GTCTC 3 cut(s) 289, 491, 808
BsnI GGCC 3 cut(s) 415, 475, 738
Bsp119I TTCGAA 1 cut(s) 919
Bsp1286I GDGCHC 3 cut(s) 23, 30, 311
Bsp143I GATC 6 cut(s) 82, 300, 565, 680, 785, 946
Bsp1720I GCTNAGC 1 cut(s) 310
BspACI CCGC 2 cut(s) 171, 673
BspANI GGCC 3 cut(s) 415, 475, 738
BspCNI CTCAG 6 cut(s) 35, 216, 281, 299, 323, 503
BspLI GGNNCC 1 cut(s) 914
BspPI GGATC 3 cut(s) 77, 573, 688
BspT104I TTCGAA 1 cut(s) 919
BspT107I GGYRCC 1 cut(s) 912
BspTI CTTAAG 1 cut(s) 667
BsrFI RCCGGY 1 cut(s) 909
BsrI ACTGG 1 cut(s) 889
BssAI RCCGGY 1 cut(s) 909
BssECI CCNNGG 3 cut(s) 397, 676, 965
BssMI GATC 6 cut(s) 82, 300, 565, 680, 785, 946
BssT1I CCWWGG 1 cut(s) 397
Bst2UI CCWGG 3 cut(s) 477, 678, 966
Bst4CI ACNGT 3 cut(s) 619, 1062, 1074
Bst6I CTCTTC 2 cut(s) 541, 1055
BstAFI CTTAAG 1 cut(s) 667
BstAPI GCANNNNNTGC 1 cut(s) 500
BstBI TTCGAA 1 cut(s) 919
BstC8I GCNNGC 3 cut(s) 294, 505, 1080
BstDEI CTNAG 6 cut(s) 22, 203, 268, 286, 310, 490
BstF5I GGATG 4 cut(s) 107, 191, 466, 582
BstKTI GATC 6 cut(s) 85, 303, 568, 683, 788, 949
BstMAI GTCTC 3 cut(s) 289, 491, 808
BstMBI GATC 6 cut(s) 82, 300, 565, 680, 785, 946
BstMWI GCNNNNNNNGC 2 cut(s) 258, 500
BstNI CCWGG 3 cut(s) 477, 678, 966
BstNSI RCATGY 1 cut(s) 716
BstSCI CCNGG 4 cut(s) 475, 624, 676, 964
BstV1I GCAGC 2 cut(s) 116, 245
BstV2I GAAGAC 2 cut(s) 49, 224
BstX2I RGATCY 1 cut(s) 82
BstYI RGATCY 1 cut(s) 82
BsuI GTATCC 1 cut(s) 622
BsuRI GGCC 3 cut(s) 415, 475, 738
BtsCI GGATG 4 cut(s) 107, 191, 466, 582
BtsIMutI CAGTG 3 cut(s) 294, 1067, 1079
Cac8I GCNNGC 3 cut(s) 294, 505, 1080
CaiI CAGNNNCTG 1 cut(s) 500
Cfr10I RCCGGY 1 cut(s) 909
Cfr13I GGNCC 2 cut(s) 413, 473
CseI GACGC 1 cut(s) 584
Csp6I GTAC 1 cut(s) 882
CviAII CATG 5 cut(s) 31, 713, 722, 793, 832
CviQI GTAC 1 cut(s) 882
DdeI CTNAG 6 cut(s) 22, 203, 268, 286, 310, 490
DpnI GATC 6 cut(s) 84, 302, 567, 682, 787, 948
DpnII GATC 6 cut(s) 82, 300, 565, 680, 785, 946
Eam1104I CTCTTC 2 cut(s) 541, 1055
EarI CTCTTC 2 cut(s) 541, 1055
Ecl136II GAGCTC 2 cut(s) 21, 309
Eco130I CCWWGG 1 cut(s) 397
Eco147I AGGCCT 1 cut(s) 738
Eco24I GRGCYC 2 cut(s) 23, 311
Eco53kI GAGCTC 2 cut(s) 21, 309
Eco57I CTGAAG 1 cut(s) 753
EcoICRI GAGCTC 2 cut(s) 21, 309
EcoRI GAATTC 1 cut(s) 659
EcoRII CCWGG 3 cut(s) 475, 676, 964
EcoT14I CCWWGG 1 cut(s) 397
EcoT38I GRGCYC 2 cut(s) 23, 311
ErhI CCWWGG 1 cut(s) 397
FaeI CATG 5 cut(s) 34, 716, 725, 796, 835
FalI AAGNNNNNCTT 2 cut(s) 209, 241
FatI CATG 5 cut(s) 30, 712, 721, 792, 831
FauNDI CATATG 1 cut(s) 838
FbaI TGATCA 1 cut(s) 300
FblI GTMKAC 1 cut(s) 1030
Fnu4HI GCNGC 3 cut(s) 130, 259, 673
FokI GGATG 4 cut(s) 94, 178, 473, 589
FriOI GRGCYC 2 cut(s) 23, 311
Fsp4HI GCNGC 3 cut(s) 130, 259, 673
FspBI CTAG 3 cut(s) 528, 857, 1132
GluI GCNGC 3 cut(s) 130, 259, 673
HaeIII GGCC 3 cut(s) 415, 475, 738
HapII CCGG 2 cut(s) 626, 910
HgaI GACGC 1 cut(s) 584
Hin1II CATG 5 cut(s) 34, 716, 725, 796, 835
HindIII AAGCTT 1 cut(s) 929
HinfI GANTC 1 cut(s) 647
HpaII CCGG 2 cut(s) 626, 910
HphI GGTGA 4 cut(s) 160, 346, 872, 954
Hpy166II GTNNAC 3 cut(s) 622, 1031, 1043
Hpy188I TCNGA 6 cut(s) 25, 76, 248, 652, 685, 790
Hpy188III TCNNGA 7 cut(s) 304, 367, 458, 543, 644, 731, 998
Hpy8I GTNNAC 3 cut(s) 622, 1031, 1043
HpyAV CCTTC 4 cut(s) 317, 325, 728, 926
HpyCH4III ACNGT 3 cut(s) 619, 1062, 1074
HpyCH4V TGCA 4 cut(s) 132, 261, 440, 987
HpyF10VI GCNNNNNNNGC 2 cut(s) 258, 500
HpyF3I CTNAG 6 cut(s) 22, 203, 268, 286, 310, 490
Hsp92II CATG 5 cut(s) 34, 716, 725, 796, 835
Ksp22I TGATCA 1 cut(s) 300
Kzo9I GATC 6 cut(s) 82, 300, 565, 680, 785, 946
LmnI GCTCC 2 cut(s) 306, 592
Lsp1109I GCAGC 2 cut(s) 116, 245
LweI GCATC 1 cut(s) 996
MaeI CTAG 3 cut(s) 528, 857, 1132
MaeIII GTNAC 1 cut(s) 951
MalI GATC 6 cut(s) 84, 302, 567, 682, 787, 948
MboI GATC 6 cut(s) 82, 300, 565, 680, 785, 946
MflI RGATCY 1 cut(s) 82
MhlI GDGCHC 3 cut(s) 23, 30, 311
MluCI AATT 7 cut(s) 56, 433, 480, 659, 867, 924, 1036
MmeI TCCRAC 1 cut(s) 186
MroXI GAANNNNTTC 2 cut(s) 374, 538
MseI TTAA 6 cut(s) 59, 351, 431, 668, 890, 927
MspA1I CMGCKG 1 cut(s) 296
MspCI CTTAAG 1 cut(s) 667
MspI CCGG 2 cut(s) 626, 910
MspR9I CCNGG 4 cut(s) 477, 626, 678, 966
MvaI CCWGG 3 cut(s) 477, 678, 966
MwoI GCNNNNNNNGC 2 cut(s) 258, 500
NciI CCSGG 1 cut(s) 626
NdeI CATATG 1 cut(s) 838
NdeII GATC 6 cut(s) 82, 300, 565, 680, 785, 946
NlaIII CATG 5 cut(s) 34, 716, 725, 796, 835
NlaIV GGNNCC 1 cut(s) 914
NmuCI GTSAC 1 cut(s) 951
NspI RCATGY 1 cut(s) 716
NspV TTCGAA 1 cut(s) 919
PceI AGGCCT 1 cut(s) 738
PciI ACATGT 1 cut(s) 712
PdmI GAANNNNTTC 2 cut(s) 374, 538
PfeI GAWTC 1 cut(s) 647
PflMI CCANNNNNTGG 1 cut(s) 403
PkrI GCNGC 3 cut(s) 131, 260, 674
PscI ACATGT 1 cut(s) 712
PshBI ATTAAT 1 cut(s) 59
Psp124BI GAGCTC 2 cut(s) 23, 311
Psp6I CCWGG 3 cut(s) 475, 676, 964
PspGI CCWGG 3 cut(s) 475, 676, 964
PspN4I GGNNCC 1 cut(s) 914
PspPI GGNCC 2 cut(s) 413, 473
PstNI CAGNNNCTG 1 cut(s) 500
PsuI RGATCY 1 cut(s) 82
PvuII CAGCTG 1 cut(s) 296
RsaI GTAC 1 cut(s) 883
RsaNI GTAC 1 cut(s) 882
SacI GAGCTC 2 cut(s) 23, 311
SaqAI TTAA 6 cut(s) 59, 351, 431, 668, 890, 927
SatI GCNGC 3 cut(s) 130, 259, 673
Sau3AI GATC 6 cut(s) 82, 300, 565, 680, 785, 946
Sau96I GGNCC 2 cut(s) 413, 473
ScaI AGTACT 1 cut(s) 883
ScrFI CCNGG 4 cut(s) 477, 626, 678, 966
SduI GDGCHC 3 cut(s) 23, 30, 311
SfaNI GCATC 1 cut(s) 996
SfuI TTCGAA 1 cut(s) 919
SmlI CTYRAG 3 cut(s) 196, 667, 851
SmoI CTYRAG 3 cut(s) 196, 667, 851
Sse9I AATT 7 cut(s) 56, 433, 480, 659, 867, 924, 1036
SseBI AGGCCT 1 cut(s) 738
SsiI CCGC 2 cut(s) 171, 673
SspMI CTAG 3 cut(s) 528, 857, 1132
SstI GAGCTC 2 cut(s) 23, 311
StuI AGGCCT 1 cut(s) 738
StyD4I CCNGG 4 cut(s) 475, 624, 676, 964
StyI CCWWGG 1 cut(s) 397
TaaI ACNGT 3 cut(s) 619, 1062, 1074
TaqI TCGA 2 cut(s) 709, 919
TaqII GACCGA 1 cut(s) 939
TasI AATT 7 cut(s) 56, 433, 480, 659, 867, 924, 1036
TatI WGTACW 1 cut(s) 881
TauI GCSGC 1 cut(s) 675
TfiI GAWTC 1 cut(s) 647
Tru1I TTAA 6 cut(s) 59, 351, 431, 668, 890, 927
Tru9I TTAA 6 cut(s) 59, 351, 431, 668, 890, 927
TscAI CASTG 3 cut(s) 294, 1067, 1079
TseFI GTSAC 1 cut(s) 951
TseI GCWGC 2 cut(s) 129, 258
Tsp45I GTSAC 1 cut(s) 951
TspDTI ATGAA 4 cut(s) 47, 198, 593, 1023
TspRI CASTG 3 cut(s) 294, 1067, 1079
Van91I CCANNNNNTGG 1 cut(s) 403
Vha464I CTTAAG 1 cut(s) 667
VspI ATTAAT 1 cut(s) 59
XapI RAATTY 2 cut(s) 433, 659
XceI RCATGY 1 cut(s) 716
XmiI GTMKAC 1 cut(s) 1030
XmnI GAANNNNTTC 2 cut(s) 374, 538
XspI CTAG 3 cut(s) 528, 857, 1132
ZrmI AGTACT 1 cut(s) 883
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.