Rw7G001900

RING-H2 zinc finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0007225
Physical Location & Seq
Reverse (-)
1558958 .. 1561331
2374 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G001900.1

Sequence Viewer

Length: 1077 bp
ATGCCTGTTCTTACAGAGAGCTCAGAGCACATGAAATGGAGAAGACCCAGAAGCCAATTAATCCAGCCCATTTCAGAAACAGATCCATACAACCCAAATCCAGCACCATCCATAATCCAATCCACTCGCTGCAAATCCACAATCTCCTCCCTCCTCCTCTCCAACTTCACCACCACCACCAATGAAGCCTCATCCTCAAGCCTCAGCACCAAGAAGAAGACAAACTTCCCCTCATCAGCAAAGTTCAGAGGCCTGGGCTGCACAGCCTCAGCCTCACAGCAAGTCTCAGTGCCAGCTGTGATCAGGAGCTCAGCAGATTGGGAAGGGAAGAAGGTGAAGAAGAAAAAGGTTAATAAGAAGAATGTCAGGAATGAGTTCAAGGACAAGAGCCAGAACCAAGGAGTGGTTGATGGGCCTGTTGGGTTTGGGTTGAATTCTGCAACTTGTATGGATTTTCAGGATGTTTGGTGTGGGCCTGGAATTGGGTTCTCAGCAGAGACTGCTGGCTCTGTGGATTGTGTTGTGGCTAGGAGAAATCGTCCTTGTTTTGCAAGGCGAACTGTGAACCCGGATACTATCTCGTTTCTTGATTCTGAACCTGAATTCCTCTTAAGCCGCCCTGGATCAGAGGTGTTTGGAAGTAGGTGTTATCGACATGTTCGCCATGCCTCTCCTGAAGGCCTCGCAGAGATTATGATGTTTCAAAGTAGTCTTATGATGGGTGGAAGATCAGACATAGATAGATATAGAGAGTGGAGACTTGATGTTGATAACATGACATATGAGGAACTGCTTGAGCTAGGTGAGAGAATTGGTTATGTGAGTACTGGATTAAAAGAAGATGAGATAAGCCGGTGCCTTCGAAAAATTAAGCTTTCAATGTTGAGTGATCGGTCACAACATTCACCTGGGCAAATAGATGGCAAGTGCATCATTTGTCAGGAGGAATATGAAGCAGATGATGAGATGGGTAGACTGAATTGTGGACACCTCTTCCACTTACAGTGTATAGAACAGTGGCTTGCTCATAAAAATACTTGCCCATTTTGTAAGGTTGAAGCGACAACTAAACGCTAG

Protein Analysis

358

Amino Acids

40.11

Weight (kDa)

8.59

Isoelectric Point (pI)

48.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 305 - 351 1e-10 RING-H2 zinc finger domain
zf-RING_2 PF13639 309 - 351 7.3e-15 Ring finger domain
zf-C3HC4_2 PF13923 309 - 350 3.1e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 309 - 351 3.4e-06 zinc-RING finger domain
zf-C3HC4 PF00097 310 - 350 2e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 310 - 348 5.1e-07 RING-type zinc-finger
zf-RING_11 PF17123 310 - 337 2.3e-06 RING-like zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 855
AccB7I CCANNNNNTGG 1 cut(s) 403
AccI GTMKAC 1 cut(s) 973
AciI CCGC 1 cut(s) 616
AclWI GGATC 2 cut(s) 77, 631
AcsI RAATTY 2 cut(s) 433, 602
AcuI CTGAAG 1 cut(s) 696
AfaI GTAC 1 cut(s) 826
AfiI CCNNNNNNNGG 2 cut(s) 403, 482
AflII CTTAAG 1 cut(s) 610
AflIII ACRYGT 1 cut(s) 655
AgsI TTSAA 5 cut(s) 379, 433, 704, 879, 1058
AjnI CCWGG 4 cut(s) 252, 475, 619, 907
AloI GAACNNNNNNTCC 2 cut(s) 588, 620
AluBI AGCT 5 cut(s) 21, 296, 309, 799, 874
AluI AGCT 5 cut(s) 21, 296, 309, 799, 874
Alw21I GWGCWC 3 cut(s) 23, 30, 311
Alw26I GTCTC 3 cut(s) 289, 491, 751
AlwI GGATC 2 cut(s) 77, 631
AlwNI CAGNNNCTG 1 cut(s) 500
AoxI GGCC 4 cut(s) 250, 413, 473, 679
ApeKI GCWGC 2 cut(s) 129, 258
ApoI RAATTY 2 cut(s) 433, 602
AseI ATTAAT 1 cut(s) 59
Asp700I GAANNNNTTC 1 cut(s) 374
AspS9I GGNCC 2 cut(s) 413, 473
AsuC2I CCSGG 1 cut(s) 569
AsuHPI GGTGA 4 cut(s) 160, 346, 815, 897
AsuII TTCGAA 1 cut(s) 862
BanI GGYRCC 1 cut(s) 855
BanII GRGCYC 2 cut(s) 23, 311
BbsI GAAGAC 2 cut(s) 49, 224
Bbv12I GWGCWC 3 cut(s) 23, 30, 311
BbvCI CCTCAGC 2 cut(s) 203, 268
BbvI GCAGC 2 cut(s) 116, 245
BccI CCATC 5 cut(s) 115, 404, 712, 914, 961
BciT130I CCWGG 4 cut(s) 254, 477, 621, 909
BciVI GTATCC 1 cut(s) 565
BclI TGATCA 1 cut(s) 300
BcnI CCSGG 1 cut(s) 569
BcoDI GTCTC 3 cut(s) 289, 491, 751
BfaI CTAG 3 cut(s) 528, 800, 1075
BfrI CTTAAG 1 cut(s) 610
BfuI GTATCC 1 cut(s) 565
BisI GCNGC 3 cut(s) 130, 259, 616
BlpI GCTNAGC 1 cut(s) 310
BlsI GCNGC 3 cut(s) 131, 260, 617
BmcAI AGTACT 1 cut(s) 826
Bme1390I CCNGG 5 cut(s) 254, 477, 569, 621, 909
BmgT120I GGNCC 2 cut(s) 413, 473
BmiI GGNNCC 1 cut(s) 857
BmrFI CCNGG 5 cut(s) 254, 477, 569, 621, 909
BmsI GCATC 1 cut(s) 939
BpiI GAAGAC 2 cut(s) 49, 224
Bpu10I CCTNAGC 2 cut(s) 203, 268
Bpu1102I GCTNAGC 1 cut(s) 310
Bpu14I TTCGAA 1 cut(s) 862
BpuEI CTTGAG 2 cut(s) 181, 815
BpuMI CCSGG 1 cut(s) 569
BsaJI CCNNGG 4 cut(s) 253, 397, 619, 908
BsaXI ACNNNNNCTCC 2 cut(s) 523, 553
Bsc4I CCNNNNNNNGG 2 cut(s) 403, 482
Bse118I RCCGGY 1 cut(s) 852
Bse1I ACTGG 1 cut(s) 832
BseBI CCWGG 4 cut(s) 254, 477, 621, 909
BseDI CCNNGG 4 cut(s) 253, 397, 619, 908
BseGI GGATG 3 cut(s) 107, 191, 466
BseLI CCNNNNNNNGG 2 cut(s) 403, 482
BseMII CTCAG 6 cut(s) 36, 217, 282, 300, 324, 504
BseNI ACTGG 1 cut(s) 832
BseRI GAGGAG 3 cut(s) 136, 143, 146
BseXI GCAGC 2 cut(s) 116, 245
BsgI GTGCAG 1 cut(s) 244
BshFI GGCC 4 cut(s) 252, 415, 475, 681
BshNI GGYRCC 1 cut(s) 855
BsiHKAI GWGCWC 3 cut(s) 23, 30, 311
BsiSI CCGG 2 cut(s) 569, 853
BslI CCNNNNNNNGG 2 cut(s) 403, 482
BsmAI GTCTC 3 cut(s) 289, 491, 751
BsnI GGCC 4 cut(s) 252, 415, 475, 681
Bsp119I TTCGAA 1 cut(s) 862
Bsp1286I GDGCHC 3 cut(s) 23, 30, 311
Bsp143I GATC 5 cut(s) 82, 300, 623, 728, 889
Bsp1720I GCTNAGC 1 cut(s) 310
BspACI CCGC 1 cut(s) 616
BspANI GGCC 4 cut(s) 252, 415, 475, 681
BspCNI CTCAG 6 cut(s) 35, 216, 281, 299, 323, 503
BspLI GGNNCC 1 cut(s) 857
BspPI GGATC 2 cut(s) 77, 631
BspT104I TTCGAA 1 cut(s) 862
BspT107I GGYRCC 1 cut(s) 855
BspTI CTTAAG 1 cut(s) 610
BsrFI RCCGGY 1 cut(s) 852
BsrI ACTGG 1 cut(s) 832
BssAI RCCGGY 1 cut(s) 852
BssECI CCNNGG 4 cut(s) 253, 397, 619, 908
BssMI GATC 5 cut(s) 82, 300, 623, 728, 889
BssT1I CCWWGG 1 cut(s) 397
Bst2UI CCWGG 4 cut(s) 254, 477, 621, 909
Bst4CI ACNGT 3 cut(s) 562, 1005, 1017
Bst6I CTCTTC 1 cut(s) 998
BstAFI CTTAAG 1 cut(s) 610
BstAPI GCANNNNNTGC 1 cut(s) 500
BstBI TTCGAA 1 cut(s) 862
BstC8I GCNNGC 3 cut(s) 294, 505, 1023
BstDEI CTNAG 6 cut(s) 22, 203, 268, 286, 310, 490
BstF5I GGATG 3 cut(s) 107, 191, 466
BstKTI GATC 5 cut(s) 85, 303, 626, 731, 892
BstMAI GTCTC 3 cut(s) 289, 491, 751
BstMBI GATC 5 cut(s) 82, 300, 623, 728, 889
BstMWI GCNNNNNNNGC 2 cut(s) 258, 500
BstNI CCWGG 4 cut(s) 254, 477, 621, 909
BstNSI RCATGY 1 cut(s) 659
BstSCI CCNGG 5 cut(s) 252, 475, 567, 619, 907
BstV1I GCAGC 2 cut(s) 116, 245
BstV2I GAAGAC 2 cut(s) 49, 224
BstX2I RGATCY 1 cut(s) 82
BstYI RGATCY 1 cut(s) 82
BsuI GTATCC 1 cut(s) 565
BsuRI GGCC 4 cut(s) 252, 415, 475, 681
BtsCI GGATG 3 cut(s) 107, 191, 466
BtsIMutI CAGTG 3 cut(s) 294, 1010, 1022
Cac8I GCNNGC 3 cut(s) 294, 505, 1023
CaiI CAGNNNCTG 1 cut(s) 500
Cfr10I RCCGGY 1 cut(s) 852
Cfr13I GGNCC 2 cut(s) 413, 473
Csp6I GTAC 1 cut(s) 825
CviAII CATG 4 cut(s) 31, 656, 665, 775
CviQI GTAC 1 cut(s) 825
DdeI CTNAG 6 cut(s) 22, 203, 268, 286, 310, 490
DpnI GATC 5 cut(s) 84, 302, 625, 730, 891
DpnII GATC 5 cut(s) 82, 300, 623, 728, 889
Eam1104I CTCTTC 1 cut(s) 998
EarI CTCTTC 1 cut(s) 998
Ecl136II GAGCTC 2 cut(s) 21, 309
Eco130I CCWWGG 1 cut(s) 397
Eco147I AGGCCT 2 cut(s) 252, 681
Eco24I GRGCYC 2 cut(s) 23, 311
Eco53kI GAGCTC 2 cut(s) 21, 309
Eco57I CTGAAG 1 cut(s) 696
EcoICRI GAGCTC 2 cut(s) 21, 309
EcoRI GAATTC 2 cut(s) 433, 602
EcoRII CCWGG 4 cut(s) 252, 475, 619, 907
EcoT14I CCWWGG 1 cut(s) 397
EcoT38I GRGCYC 2 cut(s) 23, 311
ErhI CCWWGG 1 cut(s) 397
FaeI CATG 4 cut(s) 34, 659, 668, 778
FalI AAGNNNNNCTT 2 cut(s) 209, 241
FatI CATG 4 cut(s) 30, 655, 664, 774
FauNDI CATATG 1 cut(s) 781
FbaI TGATCA 1 cut(s) 300
FblI GTMKAC 1 cut(s) 973
Fnu4HI GCNGC 3 cut(s) 130, 259, 616
FokI GGATG 3 cut(s) 94, 178, 473
FriOI GRGCYC 2 cut(s) 23, 311
Fsp4HI GCNGC 3 cut(s) 130, 259, 616
FspBI CTAG 3 cut(s) 528, 800, 1075
GluI GCNGC 3 cut(s) 130, 259, 616
HaeIII GGCC 4 cut(s) 252, 415, 475, 681
HapII CCGG 2 cut(s) 569, 853
Hin1II CATG 4 cut(s) 34, 659, 668, 778
HindIII AAGCTT 1 cut(s) 872
HinfI GANTC 1 cut(s) 590
HpaII CCGG 2 cut(s) 569, 853
HphI GGTGA 4 cut(s) 160, 346, 815, 897
Hpy166II GTNNAC 3 cut(s) 565, 974, 986
Hpy188I TCNGA 6 cut(s) 25, 76, 248, 595, 628, 733
Hpy188III TCNNGA 6 cut(s) 304, 367, 458, 587, 674, 941
Hpy8I GTNNAC 3 cut(s) 565, 974, 986
HpyAV CCTTC 4 cut(s) 317, 325, 671, 869
HpyCH4III ACNGT 3 cut(s) 562, 1005, 1017
HpyCH4V TGCA 5 cut(s) 132, 261, 440, 551, 930
HpyF10VI GCNNNNNNNGC 2 cut(s) 258, 500
HpyF3I CTNAG 6 cut(s) 22, 203, 268, 286, 310, 490
Hsp92II CATG 4 cut(s) 34, 659, 668, 778
Ksp22I TGATCA 1 cut(s) 300
Kzo9I GATC 5 cut(s) 82, 300, 623, 728, 889
LmnI GCTCC 1 cut(s) 306
Lsp1109I GCAGC 2 cut(s) 116, 245
LweI GCATC 1 cut(s) 939
MaeI CTAG 3 cut(s) 528, 800, 1075
MaeIII GTNAC 1 cut(s) 894
MalI GATC 5 cut(s) 84, 302, 625, 730, 891
MboI GATC 5 cut(s) 82, 300, 623, 728, 889
MflI RGATCY 1 cut(s) 82
MhlI GDGCHC 3 cut(s) 23, 30, 311
MluCI AATT 7 cut(s) 56, 433, 480, 602, 810, 867, 979
MmeI TCCRAC 1 cut(s) 186
MroXI GAANNNNTTC 1 cut(s) 374
MseI TTAA 5 cut(s) 59, 351, 611, 833, 870
MspA1I CMGCKG 1 cut(s) 296
MspCI CTTAAG 1 cut(s) 610
MspI CCGG 2 cut(s) 569, 853
MspR9I CCNGG 5 cut(s) 254, 477, 569, 621, 909
MvaI CCWGG 4 cut(s) 254, 477, 621, 909
MwoI GCNNNNNNNGC 2 cut(s) 258, 500
NciI CCSGG 1 cut(s) 569
NdeI CATATG 1 cut(s) 781
NdeII GATC 5 cut(s) 82, 300, 623, 728, 889
NlaIII CATG 4 cut(s) 34, 659, 668, 778
NlaIV GGNNCC 1 cut(s) 857
NmuCI GTSAC 1 cut(s) 894
NspI RCATGY 1 cut(s) 659
NspV TTCGAA 1 cut(s) 862
PceI AGGCCT 2 cut(s) 252, 681
PciI ACATGT 1 cut(s) 655
PdmI GAANNNNTTC 1 cut(s) 374
PfeI GAWTC 1 cut(s) 590
PflMI CCANNNNNTGG 1 cut(s) 403
PkrI GCNGC 3 cut(s) 131, 260, 617
PscI ACATGT 1 cut(s) 655
PshBI ATTAAT 1 cut(s) 59
Psp124BI GAGCTC 2 cut(s) 23, 311
Psp6I CCWGG 4 cut(s) 252, 475, 619, 907
PspGI CCWGG 4 cut(s) 252, 475, 619, 907
PspN4I GGNNCC 1 cut(s) 857
PspPI GGNCC 2 cut(s) 413, 473
PstNI CAGNNNCTG 1 cut(s) 500
PsuI RGATCY 1 cut(s) 82
PvuII CAGCTG 1 cut(s) 296
RsaI GTAC 1 cut(s) 826
RsaNI GTAC 1 cut(s) 825
SacI GAGCTC 2 cut(s) 23, 311
SaqAI TTAA 5 cut(s) 59, 351, 611, 833, 870
SatI GCNGC 3 cut(s) 130, 259, 616
Sau3AI GATC 5 cut(s) 82, 300, 623, 728, 889
Sau96I GGNCC 2 cut(s) 413, 473
ScaI AGTACT 1 cut(s) 826
ScrFI CCNGG 5 cut(s) 254, 477, 569, 621, 909
SduI GDGCHC 3 cut(s) 23, 30, 311
SfaNI GCATC 1 cut(s) 939
SfuI TTCGAA 1 cut(s) 862
SmlI CTYRAG 3 cut(s) 196, 610, 794
SmoI CTYRAG 3 cut(s) 196, 610, 794
Sse9I AATT 7 cut(s) 56, 433, 480, 602, 810, 867, 979
SseBI AGGCCT 2 cut(s) 252, 681
SsiI CCGC 1 cut(s) 616
SspMI CTAG 3 cut(s) 528, 800, 1075
SstI GAGCTC 2 cut(s) 23, 311
StuI AGGCCT 2 cut(s) 252, 681
StyD4I CCNGG 5 cut(s) 252, 475, 567, 619, 907
StyI CCWWGG 1 cut(s) 397
TaaI ACNGT 3 cut(s) 562, 1005, 1017
TaqI TCGA 2 cut(s) 652, 862
TaqII GACCGA 1 cut(s) 882
TasI AATT 7 cut(s) 56, 433, 480, 602, 810, 867, 979
TatI WGTACW 1 cut(s) 824
TauI GCSGC 1 cut(s) 618
TfiI GAWTC 1 cut(s) 590
Tru1I TTAA 5 cut(s) 59, 351, 611, 833, 870
Tru9I TTAA 5 cut(s) 59, 351, 611, 833, 870
TscAI CASTG 3 cut(s) 294, 1010, 1022
TseFI GTSAC 1 cut(s) 894
TseI GCWGC 2 cut(s) 129, 258
Tsp45I GTSAC 1 cut(s) 894
TspDTI ATGAA 3 cut(s) 47, 198, 966
TspRI CASTG 3 cut(s) 294, 1010, 1022
Van91I CCANNNNNTGG 1 cut(s) 403
Vha464I CTTAAG 1 cut(s) 610
VspI ATTAAT 1 cut(s) 59
XapI RAATTY 2 cut(s) 433, 602
XceI RCATGY 1 cut(s) 659
XmiI GTMKAC 1 cut(s) 973
XmnI GAANNNNTTC 1 cut(s) 374
XspI CTAG 3 cut(s) 528, 800, 1075
ZrmI AGTACT 1 cut(s) 826
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.