Rh7BG023100

RING-H2 zinc finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0007225
Physical Location & Seq
Reverse (-)
1631070 .. 1633870
2801 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG023100.1

Sequence Viewer

Length: 1137 bp
ATGCCTGTTCTTACAGAGAGCTCAGAGCACATGAAATGGAGAAGACCCAGAAGCCAATTAATCCAGCCCATTTCAGAAACAGATCCATACAACCCAAATCCAGCACCATCCATAATCCAATCCACTCGCTGCAAATCCACAATCTCCTCCCTCCTCCTCTCCAGCTTCACCACCACCACCACCAATGAAGCCTCATCCTCAAGCCTCAGCACCAAGAAGAAGACAAACTTCCCCTCACCAGCAAAGTTCAGAGGCTTGGGCTGCACAGCCTCAGCCTCACAGCAAGTCTCAGTGCCAGCTGTGATCAGGAGCTCAGCAGATTGGGAAGGGAAGAAGGTGAAGAAGAAAAAGGTTAATAAGAAGAATGTCAGGAATGAGTTCAAGGACAAGAGCCAGAACCAAGGAGTGGTTGATGGGCCTGTTGGGTTTGGGTTGAATTCTGCAACTTGTATGGATTTTCAGGATGTTTGGTGTGGGCCTGGAATTGGGTTCTCAGCAGAGACTGCTGGCTCTGTGGATTGTGTTGTGGCTAGGAGAAATGTTTCTGGAAGAGGCAAGATTGATGGGGATCATAATAGGATGAACAGCCACAGGGAGCGTCCTTGTTTAGCAAGGCGAACTGTGAACCCGGATACTATCTCGTTTCTTGATTCTGAACCTGAATTCCTCTTAAGCCGCCCTGGATCAGAGGTGTTTGGAAGTAGGTGTTATCGACATGTTCGCCATGCCTCTCCTGAAGGCCTCGCAGAGATTATGATGTTTCAAAGTAGTCTTATGATGGGTGGAAGATCAGACATGGATAGATATAGAGAGTGGAGACTTGATGTTGATAACATGACATACGAGGAACTGCTTGAGTTAGGTGAGAGAATTGGTTATGTGAGTACTGGATTAAAAGAAGATGAGATAAGCCGGTGCCTTCGAAAAATTAAGCTTTCAATGTTGAGTGATCGGTCACAACATTCACCTGGGCAAATAGATGGCAAGTGCATCATTTGTCAGGAGGAATATGAAGCAGATGATGAGATGGGTAGACTGAATTGTGGACACCTCTTCCACTTACAGTGTATAGAACAGTGGCTTGCTCATAAAAATACTTGCCCTTTTTGTAAGGTTGAAGCGACAACTAAACGCTAG

Protein Analysis

378

Amino Acids

42.33

Weight (kDa)

8.69

Isoelectric Point (pI)

49.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 325 - 371 1.1e-10 RING-H2 zinc finger domain
zf-RING_2 PF13639 329 - 371 7.9e-15 Ring finger domain
zf-C3HC4_2 PF13923 329 - 370 3.4e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 329 - 371 3.7e-06 zinc-RING finger domain
zf-C3HC4 PF00097 330 - 370 2.1e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 330 - 368 5.5e-07 RING-type zinc-finger
zf-RING_11 PF17123 330 - 357 2.5e-06 RING-like zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 915
AccB7I CCANNNNNTGG 1 cut(s) 406
AccI GTMKAC 1 cut(s) 1033
AciI CCGC 1 cut(s) 676
AclWI GGATC 3 cut(s) 77, 576, 691
AcsI RAATTY 2 cut(s) 436, 662
AcuI CTGAAG 1 cut(s) 756
AfaI GTAC 1 cut(s) 886
AfiI CCNNNNNNNGG 2 cut(s) 406, 485
AflII CTTAAG 1 cut(s) 670
AflIII ACRYGT 1 cut(s) 715
AgsI TTSAA 5 cut(s) 382, 436, 764, 939, 1118
AjnI CCWGG 3 cut(s) 478, 679, 967
AloI GAACNNNNNNTCC 2 cut(s) 648, 680
AluBI AGCT 5 cut(s) 21, 165, 299, 312, 934
AluI AGCT 5 cut(s) 21, 165, 299, 312, 934
Alw21I GWGCWC 3 cut(s) 23, 30, 314
Alw26I GTCTC 3 cut(s) 292, 494, 811
AlwI GGATC 3 cut(s) 77, 576, 691
AlwNI CAGNNNCTG 1 cut(s) 503
AoxI GGCC 3 cut(s) 416, 476, 739
ApeKI GCWGC 2 cut(s) 129, 261
ApoI RAATTY 2 cut(s) 436, 662
AseI ATTAAT 1 cut(s) 59
Asp700I GAANNNNTTC 2 cut(s) 377, 541
AspS9I GGNCC 2 cut(s) 416, 476
AsuC2I CCSGG 1 cut(s) 629
AsuHPI GGTGA 5 cut(s) 160, 228, 349, 875, 957
AsuII TTCGAA 1 cut(s) 922
BanI GGYRCC 1 cut(s) 915
BanII GRGCYC 2 cut(s) 23, 314
BbsI GAAGAC 2 cut(s) 49, 227
Bbv12I GWGCWC 3 cut(s) 23, 30, 314
BbvCI CCTCAGC 2 cut(s) 206, 271
BbvI GCAGC 2 cut(s) 116, 248
BccI CCATC 6 cut(s) 115, 407, 557, 772, 974, 1021
BciT130I CCWGG 3 cut(s) 480, 681, 969
BciVI GTATCC 1 cut(s) 625
BclI TGATCA 1 cut(s) 303
BcnI CCSGG 1 cut(s) 629
BcoDI GTCTC 3 cut(s) 292, 494, 811
BfaI CTAG 2 cut(s) 531, 1135
BfrI CTTAAG 1 cut(s) 670
BfuI GTATCC 1 cut(s) 625
BisI GCNGC 3 cut(s) 130, 262, 676
BlpI GCTNAGC 1 cut(s) 313
BlsI GCNGC 3 cut(s) 131, 263, 677
BmcAI AGTACT 1 cut(s) 886
Bme1390I CCNGG 4 cut(s) 480, 629, 681, 969
BmgT120I GGNCC 2 cut(s) 416, 476
BmiI GGNNCC 1 cut(s) 917
BmrFI CCNGG 4 cut(s) 480, 629, 681, 969
BmsI GCATC 1 cut(s) 999
BpiI GAAGAC 2 cut(s) 49, 227
BpmI CTGGAG 1 cut(s) 145
Bpu10I CCTNAGC 2 cut(s) 206, 271
Bpu1102I GCTNAGC 1 cut(s) 313
Bpu14I TTCGAA 1 cut(s) 922
BpuEI CTTGAG 2 cut(s) 184, 875
BpuMI CCSGG 1 cut(s) 629
BsaBI GATNNNNATC 1 cut(s) 567
BsaJI CCNNGG 3 cut(s) 400, 679, 968
Bsc4I CCNNNNNNNGG 2 cut(s) 406, 485
Bse118I RCCGGY 1 cut(s) 912
Bse1I ACTGG 1 cut(s) 892
Bse8I GATNNNNATC 1 cut(s) 567
BseBI CCWGG 3 cut(s) 480, 681, 969
BseDI CCNNGG 3 cut(s) 400, 679, 968
BseGI GGATG 4 cut(s) 107, 194, 469, 585
BseJI GATNNNNATC 1 cut(s) 567
BseLI CCNNNNNNNGG 2 cut(s) 406, 485
BseMII CTCAG 6 cut(s) 36, 220, 285, 303, 327, 507
BseNI ACTGG 1 cut(s) 892
BseRI GAGGAG 3 cut(s) 136, 143, 146
BseXI GCAGC 2 cut(s) 116, 248
BsgI GTGCAG 1 cut(s) 247
BshFI GGCC 3 cut(s) 418, 478, 741
BshNI GGYRCC 1 cut(s) 915
BsiHKAI GWGCWC 3 cut(s) 23, 30, 314
BsiSI CCGG 2 cut(s) 629, 913
BslI CCNNNNNNNGG 2 cut(s) 406, 485
BsmAI GTCTC 3 cut(s) 292, 494, 811
BsnI GGCC 3 cut(s) 418, 478, 741
Bsp119I TTCGAA 1 cut(s) 922
Bsp1286I GDGCHC 3 cut(s) 23, 30, 314
Bsp143I GATC 6 cut(s) 82, 303, 568, 683, 788, 949
Bsp1720I GCTNAGC 1 cut(s) 313
BspACI CCGC 1 cut(s) 676
BspANI GGCC 3 cut(s) 418, 478, 741
BspCNI CTCAG 6 cut(s) 35, 219, 284, 302, 326, 506
BspLI GGNNCC 1 cut(s) 917
BspPI GGATC 3 cut(s) 77, 576, 691
BspT104I TTCGAA 1 cut(s) 922
BspT107I GGYRCC 1 cut(s) 915
BspTI CTTAAG 1 cut(s) 670
BsrFI RCCGGY 1 cut(s) 912
BsrI ACTGG 1 cut(s) 892
BssAI RCCGGY 1 cut(s) 912
BssECI CCNNGG 3 cut(s) 400, 679, 968
BssMI GATC 6 cut(s) 82, 303, 568, 683, 788, 949
BssT1I CCWWGG 1 cut(s) 400
Bst2UI CCWGG 3 cut(s) 480, 681, 969
Bst4CI ACNGT 3 cut(s) 622, 1065, 1077
Bst6I CTCTTC 2 cut(s) 544, 1058
BstAFI CTTAAG 1 cut(s) 670
BstAPI GCANNNNNTGC 1 cut(s) 503
BstBI TTCGAA 1 cut(s) 922
BstC8I GCNNGC 3 cut(s) 297, 508, 1083
BstDEI CTNAG 6 cut(s) 22, 206, 271, 289, 313, 493
BstF5I GGATG 4 cut(s) 107, 194, 469, 585
BstKTI GATC 6 cut(s) 85, 306, 571, 686, 791, 952
BstMAI GTCTC 3 cut(s) 292, 494, 811
BstMBI GATC 6 cut(s) 82, 303, 568, 683, 788, 949
BstMWI GCNNNNNNNGC 2 cut(s) 261, 503
BstNI CCWGG 3 cut(s) 480, 681, 969
BstNSI RCATGY 1 cut(s) 719
BstSCI CCNGG 4 cut(s) 478, 627, 679, 967
BstV1I GCAGC 2 cut(s) 116, 248
BstV2I GAAGAC 2 cut(s) 49, 227
BstX2I RGATCY 1 cut(s) 82
BstYI RGATCY 1 cut(s) 82
BsuI GTATCC 1 cut(s) 625
BsuRI GGCC 3 cut(s) 418, 478, 741
BtsCI GGATG 4 cut(s) 107, 194, 469, 585
BtsIMutI CAGTG 3 cut(s) 297, 1070, 1082
Cac8I GCNNGC 3 cut(s) 297, 508, 1083
CaiI CAGNNNCTG 1 cut(s) 503
Cfr10I RCCGGY 1 cut(s) 912
Cfr13I GGNCC 2 cut(s) 416, 476
CseI GACGC 1 cut(s) 587
Csp6I GTAC 1 cut(s) 885
CviAII CATG 5 cut(s) 31, 716, 725, 796, 835
CviQI GTAC 1 cut(s) 885
DdeI CTNAG 6 cut(s) 22, 206, 271, 289, 313, 493
DpnI GATC 6 cut(s) 84, 305, 570, 685, 790, 951
DpnII GATC 6 cut(s) 82, 303, 568, 683, 788, 949
Eam1104I CTCTTC 2 cut(s) 544, 1058
EarI CTCTTC 2 cut(s) 544, 1058
Ecl136II GAGCTC 2 cut(s) 21, 312
Eco130I CCWWGG 1 cut(s) 400
Eco147I AGGCCT 1 cut(s) 741
Eco24I GRGCYC 2 cut(s) 23, 314
Eco53kI GAGCTC 2 cut(s) 21, 312
Eco57I CTGAAG 1 cut(s) 756
EcoICRI GAGCTC 2 cut(s) 21, 312
EcoRI GAATTC 2 cut(s) 436, 662
EcoRII CCWGG 3 cut(s) 478, 679, 967
EcoT14I CCWWGG 1 cut(s) 400
EcoT38I GRGCYC 2 cut(s) 23, 314
ErhI CCWWGG 1 cut(s) 400
FaeI CATG 5 cut(s) 34, 719, 728, 799, 838
FalI AAGNNNNNCTT 2 cut(s) 212, 244
FatI CATG 5 cut(s) 30, 715, 724, 795, 834
FbaI TGATCA 1 cut(s) 303
FblI GTMKAC 1 cut(s) 1033
Fnu4HI GCNGC 3 cut(s) 130, 262, 676
FokI GGATG 4 cut(s) 94, 181, 476, 592
FriOI GRGCYC 2 cut(s) 23, 314
Fsp4HI GCNGC 3 cut(s) 130, 262, 676
FspBI CTAG 2 cut(s) 531, 1135
GluI GCNGC 3 cut(s) 130, 262, 676
GsuI CTGGAG 1 cut(s) 145
HaeIII GGCC 3 cut(s) 418, 478, 741
HapII CCGG 2 cut(s) 629, 913
HgaI GACGC 1 cut(s) 587
Hin1II CATG 5 cut(s) 34, 719, 728, 799, 838
HindIII AAGCTT 1 cut(s) 932
HinfI GANTC 1 cut(s) 650
HpaII CCGG 2 cut(s) 629, 913
HphI GGTGA 5 cut(s) 160, 228, 349, 875, 957
Hpy166II GTNNAC 3 cut(s) 625, 1034, 1046
Hpy188I TCNGA 6 cut(s) 25, 76, 251, 655, 688, 793
Hpy188III TCNNGA 7 cut(s) 307, 370, 461, 546, 647, 734, 1001
Hpy8I GTNNAC 3 cut(s) 625, 1034, 1046
HpyAV CCTTC 4 cut(s) 320, 328, 731, 929
HpyCH4III ACNGT 3 cut(s) 622, 1065, 1077
HpyCH4V TGCA 4 cut(s) 132, 264, 443, 990
HpyF10VI GCNNNNNNNGC 2 cut(s) 261, 503
HpyF3I CTNAG 6 cut(s) 22, 206, 271, 289, 313, 493
Hsp92II CATG 5 cut(s) 34, 719, 728, 799, 838
Ksp22I TGATCA 1 cut(s) 303
Kzo9I GATC 6 cut(s) 82, 303, 568, 683, 788, 949
LmnI GCTCC 2 cut(s) 309, 595
Lsp1109I GCAGC 2 cut(s) 116, 248
LweI GCATC 1 cut(s) 999
MaeI CTAG 2 cut(s) 531, 1135
MaeIII GTNAC 1 cut(s) 954
MalI GATC 6 cut(s) 84, 305, 570, 685, 790, 951
MboI GATC 6 cut(s) 82, 303, 568, 683, 788, 949
MflI RGATCY 1 cut(s) 82
MhlI GDGCHC 3 cut(s) 23, 30, 314
MluCI AATT 7 cut(s) 56, 436, 483, 662, 870, 927, 1039
MroXI GAANNNNTTC 2 cut(s) 377, 541
MseI TTAA 5 cut(s) 59, 354, 671, 893, 930
MspA1I CMGCKG 1 cut(s) 299
MspCI CTTAAG 1 cut(s) 670
MspI CCGG 2 cut(s) 629, 913
MspR9I CCNGG 4 cut(s) 480, 629, 681, 969
MvaI CCWGG 3 cut(s) 480, 681, 969
MwoI GCNNNNNNNGC 2 cut(s) 261, 503
NciI CCSGG 1 cut(s) 629
NdeII GATC 6 cut(s) 82, 303, 568, 683, 788, 949
NlaIII CATG 5 cut(s) 34, 719, 728, 799, 838
NlaIV GGNNCC 1 cut(s) 917
NmuCI GTSAC 1 cut(s) 954
NspI RCATGY 1 cut(s) 719
NspV TTCGAA 1 cut(s) 922
PceI AGGCCT 1 cut(s) 741
PciI ACATGT 1 cut(s) 715
PdmI GAANNNNTTC 2 cut(s) 377, 541
PfeI GAWTC 1 cut(s) 650
PflMI CCANNNNNTGG 1 cut(s) 406
PkrI GCNGC 3 cut(s) 131, 263, 677
PscI ACATGT 1 cut(s) 715
PshBI ATTAAT 1 cut(s) 59
Psp124BI GAGCTC 2 cut(s) 23, 314
Psp6I CCWGG 3 cut(s) 478, 679, 967
PspGI CCWGG 3 cut(s) 478, 679, 967
PspN4I GGNNCC 1 cut(s) 917
PspPI GGNCC 2 cut(s) 416, 476
PstNI CAGNNNCTG 1 cut(s) 503
PsuI RGATCY 1 cut(s) 82
PvuII CAGCTG 1 cut(s) 299
RsaI GTAC 1 cut(s) 886
RsaNI GTAC 1 cut(s) 885
SacI GAGCTC 2 cut(s) 23, 314
SaqAI TTAA 5 cut(s) 59, 354, 671, 893, 930
SatI GCNGC 3 cut(s) 130, 262, 676
Sau3AI GATC 6 cut(s) 82, 303, 568, 683, 788, 949
Sau96I GGNCC 2 cut(s) 416, 476
ScaI AGTACT 1 cut(s) 886
ScrFI CCNGG 4 cut(s) 480, 629, 681, 969
SduI GDGCHC 3 cut(s) 23, 30, 314
SfaNI GCATC 1 cut(s) 999
SfuI TTCGAA 1 cut(s) 922
SmlI CTYRAG 3 cut(s) 199, 670, 854
SmoI CTYRAG 3 cut(s) 199, 670, 854
Sse9I AATT 7 cut(s) 56, 436, 483, 662, 870, 927, 1039
SseBI AGGCCT 1 cut(s) 741
SsiI CCGC 1 cut(s) 676
SspMI CTAG 2 cut(s) 531, 1135
SstI GAGCTC 2 cut(s) 23, 314
StuI AGGCCT 1 cut(s) 741
StyD4I CCNGG 4 cut(s) 478, 627, 679, 967
StyI CCWWGG 1 cut(s) 400
TaaI ACNGT 3 cut(s) 622, 1065, 1077
TaqI TCGA 2 cut(s) 712, 922
TaqII GACCGA 1 cut(s) 942
TasI AATT 7 cut(s) 56, 436, 483, 662, 870, 927, 1039
TatI WGTACW 1 cut(s) 884
TauI GCSGC 1 cut(s) 678
TfiI GAWTC 1 cut(s) 650
Tru1I TTAA 5 cut(s) 59, 354, 671, 893, 930
Tru9I TTAA 5 cut(s) 59, 354, 671, 893, 930
TscAI CASTG 3 cut(s) 297, 1070, 1082
TseFI GTSAC 1 cut(s) 954
TseI GCWGC 2 cut(s) 129, 261
Tsp45I GTSAC 1 cut(s) 954
TspDTI ATGAA 4 cut(s) 47, 201, 596, 1026
TspRI CASTG 3 cut(s) 297, 1070, 1082
Van91I CCANNNNNTGG 1 cut(s) 406
Vha464I CTTAAG 1 cut(s) 670
VspI ATTAAT 1 cut(s) 59
XapI RAATTY 2 cut(s) 436, 662
XceI RCATGY 1 cut(s) 719
XmiI GTMKAC 1 cut(s) 1033
XmnI GAANNNNTTC 2 cut(s) 377, 541
XspI CTAG 2 cut(s) 531, 1135
ZrmI AGTACT 1 cut(s) 886
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.