Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0007225

Physical Location & Seq

Genomic Coordinates Forward (+)

31101901 .. 31109623

Gene Structure 7723 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1230200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1140 bp

ATGCCTATTCTCACAGAGAGCTCAACAGCAGGAGCAGAGCACATGAAATGGAGAAGACCCAGAACCCAATTCACCCAACCCATTTCAGAAACGGATCCAGCCACCTCCCTCCTCCAATCCACCCGCTGTAAACCCACCATCTCCTCCCTCCTCCTCTCCAGCTTCACCACCACCACAACCACCGACAACAACGAAGCCTTACCTTCAAGCCTTACGATGAAGAAAAAGACAAACTTTCCCTCGTCGACGTTCCGAGGCCTGGGCTGCGCCGCATCCGCCTCGCAGCAAGTCTCGGTGCCGGAGGTGATTCGGAACTCCGCTGATTGGAAGGGAAAGAAGGTGAAGAAGAAAAAGCAGAAGAAGAGCGCCGCCGCCAAGGGCAGCGCAAGCAATGAGAAGAATGAATACAGGGATGTCGGTGATGGTGGGCCCACCTTCGGTATAAATTCTGCAACATGTATGGATTTTCAGGATGTTTGGTGCGGCCCCGGAATTGGGTTATCGGCGGAAACTGCCGGCTCGGTGGATTGCGTGGTGGCGAGGAGGAATGTCTCCGGCAGAGGCAGGATTGCTGCGGACAATAATAAGATGAGCAGCCACAGGGAGCGTCCTTGTTTAGCAAGGCGAAACGTGAGCCCGGAAACCCTCTCGTTTCTGGATTCCGAACCGGATTTTGTCTCGAGCCGCCGTGAAACGGAGGTGTTTGGAAGTAGGTGTTTTCGGCATGTCCGGCATCCATCCCCCGAAGACCTTGCAGAGATTATGCTGTTTCAAAGTAGTCTTATGATGGGTGGAAGACTTGATAGATTTCGTGACTGGAGACTCGATGTCGATAGCATGTCGTATGAGCAACTGCTTGAGTTGGGTGAGAGAATTGGTTACGTGAGTACTGGATTAAAAGAAGATGAAATTAACCGCTGCCTTCGGAAGATTAAGCTTCCAATGTCAAGTGACGCGTCGCCACATTCATCTGGGAAAGGAGATAGCAAATGCATCATTTGTCAGGAGGAGTACGAAGCAGATGACGAGATGGGCAAGCTGCCCTGCGGACACAACTTCCACCTACAATGTATAAAACAGTGGCTTGCACAGAAGAATACTTGCCCATTTTGCAAGGTCGAAGCATTATCTAGATGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0036211 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0051603 GO:0051865 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

380

Amino Acids

41.99

Weight (kDa)

8.75

Isoelectric Point (pI)

50.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-rbx1	PF12678	328 - 372	3e-11	RING-H2 zinc finger domain
zf-C3HC4_3	PF13920	328 - 374	5.8e-06	Zinc finger, C3HC4 type (RING finger)
zf-RING_2	PF13639	330 - 372	1.9e-15	Ring finger domain
zf-C3HC4_2	PF13923	330 - 371	6.7e-09	Zinc finger, C3HC4 type (RING finger)
zf-RING_5	PF14634	330 - 372	3.6e-06	zinc-RING finger domain
zf-RING_UBOX	PF13445	331 - 369	2.7e-08	RING-type zinc-finger
zf-C3HC4	PF00097	331 - 371	5.3e-08	Zinc finger, C3HC4 type (RING finger)
zf-RING_11	PF17123	331 - 358	5.3e-07	RING-like zinc finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007225)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G17970 AT1G17970 AT1G73760 AT1G73760
fragaria_vesca	FvH4_5g16700 FvH4_5g16700
malus_domestica	MD06G1220700.v1.1 MD14G1230200.v1.1
prunus_persica	Prupe.5G225100_v2.0.a1 Prupe.5G225100_v2.0.a1
pyrus_communis	pycom06g19660 pycom14g19150
rosa_chinensis	RchiOBHm_Chr7g0179401
rosa_laevigata	RLG00000005359
rosa_multiflora	Rmu_co8089052.1_g000001 Rmu_co8438249.1_g000001 Rmu_sc0002581.1_g000003
rosa_roxburghii	Rroxscaffold_3G00274060
rosa_rugosa	Rorug06G0424100 Rorug06G0424200
rosa_samantha	Rh7AG023500 Rh7BG023100 Rh7CG024500 Rh7DG024000
rosa_wichuraiana	Rw7G001900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	295
AccI	GTMKAC	1 cut(s)	245
AccII	CGCG	1 cut(s)	956
AclWI	GGATC	2 cut(s)	89, 102
AcsI	RAATTY	1 cut(s)	445
AfaI	GTAC	2 cut(s)	889, 1013
AfiI	CCNNNNNNNGG	6 cut(s)	259, 324, 437, 494, 495, 694
AflIII	ACRYGT	2 cut(s)	455, 954
AgsI	TTSAA	2 cut(s)	207, 773
AhdI	GACNNNNNGTC	1 cut(s)	827
AjnI	CCWGG	1 cut(s)	258
AluBI	AGCT	4 cut(s)	21, 162, 937, 1039
AluI	AGCT	4 cut(s)	21, 162, 937, 1039
Alw21I	GWGCWC	2 cut(s)	23, 42
Alw26I	GTCTC	4 cut(s)	295, 556, 682, 814
AlwI	GGATC	2 cut(s)	89, 102
Ama87I	CYCGRG	1 cut(s)	679
AoxI	GGCC	3 cut(s)	256, 428, 484
ApaI	GGGCCC	1 cut(s)	432
ApeKI	GCWGC	7 cut(s)	264, 283, 381, 572, 594, 918, 1039
ApoI	RAATTY	1 cut(s)	445
AspLEI	GCGC	3 cut(s)	269, 368, 386
AspS9I	GGNCC	3 cut(s)	428, 429, 485
AsuC2I	CCSGG	2 cut(s)	489, 638
AsuHPI	GGTGA	6 cut(s)	64, 157, 316, 352, 431, 878
AvaI	CYCGRG	1 cut(s)	679
BaeGI	GKGCMC	1 cut(s)	432
BamHI	GGATCC	1 cut(s)	94
BanI	GGYRCC	1 cut(s)	295
BanII	GRGCYC	3 cut(s)	23, 432, 638
BarI	GAAGNNNNNNTAC	2 cut(s)	389, 421
BbsI	GAAGAC	3 cut(s)	61, 753, 802
Bbv12I	GWGCWC	2 cut(s)	23, 42
BbvI	GCAGC	7 cut(s)	251, 295, 393, 559, 606, 905, 1026
BccI	CCATC	5 cut(s)	146, 416, 745, 781, 1024
BceAI	ACGGC	1 cut(s)	672
BcgI	CGANNNNNNTGC	4 cut(s)	261, 295, 734, 768
BciT130I	CCWGG	1 cut(s)	260
BcnI	CCSGG	2 cut(s)	489, 638
BcoDI	GTCTC	4 cut(s)	295, 556, 682, 814
BfaI	CTAG	2 cut(s)	1131, 1138
BfoI	RGCGCY	1 cut(s)	369
BmcAI	AGTACT	1 cut(s)	889
Bme1390I	CCNGG	3 cut(s)	260, 489, 638
BmeRI	GACNNNNNGTC	1 cut(s)	827
BmeT110I	CYCGRG	1 cut(s)	679
BmgT120I	GGNCC	3 cut(s)	428, 429, 485
BmiI	GGNNCC	4 cut(s)	96, 297, 430, 487
BmrFI	CCNGG	3 cut(s)	260, 489, 638
BmsI	GCATC	4 cut(s)	281, 742, 1002, 1124
BpiI	GAAGAC	3 cut(s)	61, 753, 802
BpmI	CTGGAG	2 cut(s)	142, 838
BpuEI	CTTGAG	1 cut(s)	878
BpuMI	CCSGG	2 cut(s)	489, 638
BsaAI	YACGTR	1 cut(s)	883
BsaJI	CCNNGG	4 cut(s)	253, 259, 375, 487
BsaWI	WCCGGW	1 cut(s)	667
Bsc4I	CCNNNNNNNGG	6 cut(s)	259, 324, 437, 494, 495, 694
Bse118I	RCCGGY	1 cut(s)	515
Bse1I	ACTGG	2 cut(s)	821, 895
Bse3DI	GCAATG	1 cut(s)	397
BseBI	CCWGG	1 cut(s)	260
BseDI	CCNNGG	4 cut(s)	253, 259, 375, 487
BseGI	GGATG	5 cut(s)	272, 418, 478, 733, 737
BseLI	CCNNNNNNNGG	6 cut(s)	259, 324, 437, 494, 495, 694
BseMI	GCAATG	1 cut(s)	397
BseNI	ACTGG	2 cut(s)	821, 895
BseRI	GAGGAG	6 cut(s)	101, 133, 140, 143, 556, 1022
BseSI	GKGCMC	1 cut(s)	432
BseXI	GCAGC	7 cut(s)	251, 295, 393, 559, 606, 905, 1026
Bsh1236I	CGCG	1 cut(s)	956
BshFI	GGCC	3 cut(s)	258, 430, 486
BshNI	GGYRCC	1 cut(s)	295
BsiHKAI	GWGCWC	2 cut(s)	23, 42
BsiHKCI	CYCGRG	1 cut(s)	679
BsiSI	CCGG	7 cut(s)	299, 489, 516, 555, 638, 668, 730
BslI	CCNNNNNNNGG	6 cut(s)	259, 324, 437, 494, 495, 694
BsmAI	GTCTC	4 cut(s)	295, 556, 682, 814
BsnI	GGCC	3 cut(s)	258, 430, 486
BsoBI	CYCGRG	1 cut(s)	679
Bsp120I	GGGCCC	1 cut(s)	428
Bsp1286I	GDGCHC	4 cut(s)	23, 42, 432, 638
Bsp143I	GATC	1 cut(s)	94
BspANI	GGCC	3 cut(s)	258, 430, 486
BspFNI	CGCG	1 cut(s)	956
BspLI	GGNNCC	4 cut(s)	96, 297, 430, 487
BspPI	GGATC	2 cut(s)	89, 102
BspQI	GCTCTTC	1 cut(s)	356
BspT107I	GGYRCC	1 cut(s)	295
BsrDI	GCAATG	1 cut(s)	397
BsrFI	RCCGGY	1 cut(s)	515
BsrI	ACTGG	2 cut(s)	821, 895
BssAI	RCCGGY	1 cut(s)	515
BssECI	CCNNGG	4 cut(s)	253, 259, 375, 487
BssMI	GATC	1 cut(s)	94
BssT1I	CCWWGG	1 cut(s)	375
Bst2UI	CCWGG	1 cut(s)	260
Bst4CI	ACNGT	1 cut(s)	1080
Bst6I	CTCTTC	1 cut(s)	356
BstBAI	YACGTR	1 cut(s)	883
BstC8I	GCNNGC	4 cut(s)	388, 517, 1037, 1086
BstF5I	GGATG	5 cut(s)	272, 418, 478, 733, 737
BstFNI	CGCG	1 cut(s)	956
BstH2I	RGCGCY	1 cut(s)	369
BstHHI	GCGC	3 cut(s)	269, 368, 386
BstKTI	GATC	1 cut(s)	97
BstMAI	GTCTC	4 cut(s)	295, 556, 682, 814
BstMBI	GATC	1 cut(s)	94
BstMWI	GCNNNNNNNGC	6 cut(s)	264, 275, 387, 512, 730, 1110
BstNI	CCWGG	1 cut(s)	260
BstNSI	RCATGY	3 cut(s)	459, 728, 841
BstSCI	CCNGG	3 cut(s)	258, 487, 636
BstSLI	GKGCMC	1 cut(s)	432
BstUI	CGCG	1 cut(s)	956
BstV1I	GCAGC	7 cut(s)	251, 295, 393, 559, 606, 905, 1026
BstV2I	GAAGAC	3 cut(s)	61, 753, 802
BstX2I	RGATCY	1 cut(s)	94
BstYI	RGATCY	1 cut(s)	94
BsuRI	GGCC	3 cut(s)	258, 430, 486
BtsCI	GGATG	5 cut(s)	272, 418, 478, 733, 737
BtsIMutI	CAGTG	1 cut(s)	1085
Cac8I	GCNNGC	4 cut(s)	388, 517, 1037, 1086
CfoI	GCGC	3 cut(s)	269, 368, 386
Cfr10I	RCCGGY	1 cut(s)	515
Cfr13I	GGNCC	3 cut(s)	428, 429, 485
CseI	GACGC	3 cut(s)	596, 945, 962
Csp6I	GTAC	2 cut(s)	888, 1012
CviAII	CATG	4 cut(s)	43, 456, 725, 838
CviQI	GTAC	2 cut(s)	888, 1012
DpnI	GATC	1 cut(s)	96
DpnII	GATC	1 cut(s)	94
DriI	GACNNNNNGTC	1 cut(s)	827
Eam1104I	CTCTTC	1 cut(s)	356
Eam1105I	GACNNNNNGTC	1 cut(s)	827
EarI	CTCTTC	1 cut(s)	356
EciI	GGCGGA	2 cut(s)	265, 521
Ecl136II	GAGCTC	1 cut(s)	21
Eco130I	CCWWGG	1 cut(s)	375
Eco147I	AGGCCT	1 cut(s)	258
Eco24I	GRGCYC	3 cut(s)	23, 432, 638
Eco53kI	GAGCTC	1 cut(s)	21
Eco88I	CYCGRG	1 cut(s)	679
EcoICRI	GAGCTC	1 cut(s)	21
EcoRII	CCWGG	1 cut(s)	258
EcoT14I	CCWWGG	1 cut(s)	375
EcoT22I	ATGCAT	1 cut(s)	995
EcoT38I	GRGCYC	3 cut(s)	23, 432, 638
ErhI	CCWWGG	1 cut(s)	375
FaeI	CATG	4 cut(s)	46, 459, 728, 841
FalI	AAGNNNNNCTT	2 cut(s)	218, 250
FatI	CATG	4 cut(s)	42, 455, 724, 837
FauI	CCCGC	1 cut(s)	131
FblI	GTMKAC	1 cut(s)	245
FokI	GGATG	5 cut(s)	259, 425, 485, 720, 724
FriOI	GRGCYC	3 cut(s)	23, 432, 638
FspBI	CTAG	2 cut(s)	1131, 1138
GlaI	GCGC	3 cut(s)	268, 367, 385
GsuI	CTGGAG	2 cut(s)	142, 838
HaeII	RGCGCY	1 cut(s)	369
HaeIII	GGCC	3 cut(s)	258, 430, 486
HapII	CCGG	7 cut(s)	299, 489, 516, 555, 638, 668, 730
HgaI	GACGC	3 cut(s)	596, 945, 962
HhaI	GCGC	3 cut(s)	269, 368, 386
Hin1II	CATG	4 cut(s)	46, 459, 728, 841
Hin6I	GCGC	3 cut(s)	267, 366, 384
HinP1I	GCGC	3 cut(s)	267, 366, 384
HincII	GTYRAC	1 cut(s)	246
HindII	GTYRAC	1 cut(s)	246
HindIII	AAGCTT	1 cut(s)	935
HinfI	GANTC	3 cut(s)	307, 659, 822
HpaII	CCGG	7 cut(s)	299, 489, 516, 555, 638, 668, 730
HphI	GGTGA	6 cut(s)	64, 157, 316, 352, 431, 878
Hpy166II	GTNNAC	2 cut(s)	131, 246
Hpy188I	TCNGA	5 cut(s)	88, 254, 312, 664, 927
Hpy188III	TCNNGA	6 cut(s)	470, 656, 679, 812, 1004, 1131
Hpy8I	GTNNAC	2 cut(s)	131, 246
Hpy99I	CGWCG	3 cut(s)	247, 250, 961
HpyAV	CCTTC	5 cut(s)	213, 322, 331, 445, 932
HpyCH4III	ACNGT	1 cut(s)	1080
HpyCH4IV	ACGT	3 cut(s)	248, 630, 882
HpyCH4V	TGCA	5 cut(s)	452, 755, 993, 1088, 1113
HpyF10VI	GCNNNNNNNGC	6 cut(s)	264, 275, 387, 512, 730, 1110
HpySE526I	ACGT	3 cut(s)	248, 630, 882
Hsp92II	CATG	4 cut(s)	46, 459, 728, 841
HspAI	GCGC	3 cut(s)	267, 366, 384
KroI	GCCGGC	1 cut(s)	515
KroNI	GCCGGC	1 cut(s)	517
Kzo9I	GATC	1 cut(s)	94
LguI	GCTCTTC	1 cut(s)	356
LmnI	GCTCC	2 cut(s)	32, 604
Lsp1109I	GCAGC	7 cut(s)	251, 295, 393, 559, 606, 905, 1026
LweI	GCATC	4 cut(s)	281, 742, 1002, 1124
MaeI	CTAG	2 cut(s)	1131, 1138
MaeII	ACGT	3 cut(s)	248, 630, 882
MaeIII	GTNAC	3 cut(s)	812, 878, 950
MalI	GATC	1 cut(s)	96
MboI	GATC	1 cut(s)	94
MflI	RGATCY	1 cut(s)	94
MhlI	GDGCHC	4 cut(s)	23, 42, 432, 638
MluCI	AATT	5 cut(s)	68, 445, 492, 873, 909
MluI	ACGCGT	1 cut(s)	954
MlyI	GAGTC	1 cut(s)	816
Mph1103I	ATGCAT	1 cut(s)	995
MroNI	GCCGGC	1 cut(s)	515
MseI	TTAA	3 cut(s)	896, 912, 933
MspA1I	CMGCKG	3 cut(s)	126, 320, 918
MspI	CCGG	7 cut(s)	299, 489, 516, 555, 638, 668, 730
MspR9I	CCNGG	3 cut(s)	260, 489, 638
MvaI	CCWGG	1 cut(s)	260
MvnI	CGCG	1 cut(s)	956
MwoI	GCNNNNNNNGC	6 cut(s)	264, 275, 387, 512, 730, 1110
NaeI	GCCGGC	1 cut(s)	517
NciI	CCSGG	2 cut(s)	489, 638
NdeII	GATC	1 cut(s)	94
NgoMIV	GCCGGC	1 cut(s)	515
NlaIII	CATG	4 cut(s)	46, 459, 728, 841
NlaIV	GGNNCC	4 cut(s)	96, 297, 430, 487
NmuCI	GTSAC	2 cut(s)	812, 950
NsiI	ATGCAT	1 cut(s)	995
NspI	RCATGY	3 cut(s)	459, 728, 841
PaeR7I	CTCGAG	1 cut(s)	679
PceI	AGGCCT	1 cut(s)	258
PciI	ACATGT	1 cut(s)	455
PciSI	GCTCTTC	1 cut(s)	356
PdiI	GCCGGC	1 cut(s)	517
PfeI	GAWTC	2 cut(s)	307, 659
PleI	GAGTC	1 cut(s)	816
PpsI	GAGTC	1 cut(s)	816
Ppu21I	YACGTR	1 cut(s)	883
PscI	ACATGT	1 cut(s)	455
Psp124BI	GAGCTC	1 cut(s)	23
Psp6I	CCWGG	1 cut(s)	258
PspGI	CCWGG	1 cut(s)	258
PspN4I	GGNNCC	4 cut(s)	96, 297, 430, 487
PspOMI	GGGCCC	1 cut(s)	428
PspPI	GGNCC	3 cut(s)	428, 429, 485
PsuI	RGATCY	1 cut(s)	94
RsaI	GTAC	2 cut(s)	889, 1013
RsaNI	GTAC	2 cut(s)	888, 1012
SacI	GAGCTC	1 cut(s)	23
SalI	GTCGAC	1 cut(s)	244
SapI	GCTCTTC	1 cut(s)	356
SaqAI	TTAA	3 cut(s)	896, 912, 933
Sau3AI	GATC	1 cut(s)	94
Sau96I	GGNCC	3 cut(s)	428, 429, 485
ScaI	AGTACT	1 cut(s)	889
SchI	GAGTC	1 cut(s)	816
ScrFI	CCNGG	3 cut(s)	260, 489, 638
SduI	GDGCHC	4 cut(s)	23, 42, 432, 638
SfaNI	GCATC	4 cut(s)	281, 742, 1002, 1124
Sfr274I	CTCGAG	1 cut(s)	679
SgrDI	CGTCGACG	1 cut(s)	244
SlaI	CTCGAG	1 cut(s)	679
SmlI	CTYRAG	2 cut(s)	679, 857
SmoI	CTYRAG	2 cut(s)	679, 857
Sse9I	AATT	5 cut(s)	68, 445, 492, 873, 909
SseBI	AGGCCT	1 cut(s)	258
SspMI	CTAG	2 cut(s)	1131, 1138
SstI	GAGCTC	1 cut(s)	23
StuI	AGGCCT	1 cut(s)	258
StyD4I	CCNGG	3 cut(s)	258, 487, 636
StyI	CCWWGG	1 cut(s)	375
TaaI	ACNGT	1 cut(s)	1080
TaiI	ACGT	3 cut(s)	251, 633, 885
TaqI	TCGA	5 cut(s)	245, 680, 825, 831, 1119
TasI	AATT	5 cut(s)	68, 445, 492, 873, 909
TatI	WGTACW	1 cut(s)	887
TauI	GCSGC	5 cut(s)	272, 371, 374, 486, 687
TfiI	GAWTC	2 cut(s)	307, 659
Tru1I	TTAA	3 cut(s)	896, 912, 933
Tru9I	TTAA	3 cut(s)	896, 912, 933
TscAI	CASTG	1 cut(s)	1085
TseFI	GTSAC	2 cut(s)	812, 950
TseI	GCWGC	7 cut(s)	264, 283, 381, 572, 594, 918, 1039
Tsp45I	GTSAC	2 cut(s)	812, 950
TspDTI	ATGAA	5 cut(s)	59, 233, 417, 921, 957
TspGWI	ACGGA	2 cut(s)	107, 710
TspRI	CASTG	1 cut(s)	1085
XapI	RAATTY	1 cut(s)	445
XbaI	TCTAGA	1 cut(s)	1130
XceI	RCATGY	3 cut(s)	459, 728, 841
XhoI	CTCGAG	1 cut(s)	679
XmiI	GTMKAC	1 cut(s)	245
XspI	CTAG	2 cut(s)	1131, 1138
ZrmI	AGTACT	1 cut(s)	889
Zsp2I	ATGCAT	1 cut(s)	995

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.