Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0007322

Physical Location & Seq

Genomic Coordinates Forward (+)

35609410 .. 35610754

Gene Structure 1345 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1225300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1302 bp

ATGGCCCAAAACCCAAGACCCCCAACAATTCTCAAATCCCTGGGCGAAGAACTGATCAGAATCATCACTCCGGTCTCCGTCTGTATGCTGCTGGTGGTCCTTCTGGTCACAGTCCTAAACGATGACGCTTCATCCTCGTCGGAGACGGTGAACTCAATCGCCACCATTGCCTACGACGAGACCGCTTCGGACTCCTCCTGGGACAAATTCGTGGGTGCCCTCTTAAACGCCCTCGTTTTTGTCGCCGTCGTCACGGTCGTCACGTTCGTTTTGGTGCTCCTTTTTTACCTCCGATGCACCAGGTTCCTCAAAATCTACATGGGTTTTTCGTCTTTTGTTGTTCTGGGATTCATGGGCGGAGAAGTCGCCCTGTTTTTGATTCAGGACTTTAATGTTCCAGTCGATTCGATTACGTTTGTTTTGGTGTTGTTCAATTTTGCTGTTGTTGGGGTTTTGGCGGTGTTCATGTCGAAAATGGCGATTTTCGTGACGCAGGGCTATTTGGTTGTGATTGGAATGCTGGTTGCTTATTGGTTTACGTTGTTGCCCGAGTGGACAACTTGGGTGCTGTTGGTTGCCATGGCATTGTATGATCTTGCCGCGGTTTTGTTGCCCGTCGGGCCGTTGAGGTTGTTGGTGGAGCTCGCAATCTCTAGGGATGAGGAGATTCCGGCTCTGGTTTACGAGGCCAGGCCCGTGACTGCTCATGATTCTCAGGGTAATGTGGGTGAGAGGAGGGTTTGGAGAGATAGGAGAAATGAGAATTCGAGTAATGGGAATGAGAATCCCGGGTCAACTTCTAATGTCAATGTCGATCACGCTTCTGGTTTGAATTCAAGTTCGAATCTTGGTGAGTTTGGCAATGTTAATAATGATTCGAGTTTGGTTAGAGCCGAAGAGGGGCGGATTCCAGATAGGGGTCAAGAGCTATCTGCCCCATTACTTGAGCGTGTAGCAAATGTTCCACCTCGCAGGCAGGAAGATGTTGTGGCGAGTGAAAGCTTGTTGCTTGAGGGCATCGGATTGGGGTCCTCCGGTTCAATCAAGCTGGGATTGGGAGACTTCATCTTCTACAGTGTTTTAGTCGGGAGGGCAGCAATGTATGATTTTATGACCGTGTATGCGTGTTATCTTGCCATCATAGGTGGCCTTGGAGTTACTCTGATACTGTTAGCTTTGTATCAGAAAGCTCTGCCAGCTCTTCCGGTGTCAATAATACTCGGCGTACTGATTTATGTATTGACACGGCTCTTACTCGAAGTTTTCGTTGTACAATGTTCCTTGAACCTCATTATGTTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005798 GO:0005886 GO:0005887 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0016020 GO:0016021 GO:0016787 GO:0019538 GO:0031224 GO:0031226 GO:0031410 GO:0031982 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0070011 GO:0071704 GO:0071944 GO:0097708 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04310 ko04330 ko04722 ko05010 ko05165 map04310 map04330 map04722 map05010 map05165

Pfam Domains

Protein Families

Presenilin

Protein Analysis

434

Amino Acids

47.16

Weight (kDa)

4.7

Isoelectric Point (pI)

38.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Presenilin	PF01080	13 - 423	2.8e-129	Presenilin

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0007322)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G29900
fragaria_vesca	FvH4_5g13800 FvH4_5g13800
malus_domestica	MD06G1225300.v1.1 MD14G1212200.v1.1
prunus_persica	Prupe.5G205400_v2.0.a1
pyrus_communis	pycom06g17980 pycom14g17580 pycom15g04200
rosa_chinensis	RchiOBHm_Chr7g0183141
rosa_laevigata	RLG00000005075 RLG00000009030
rosa_multiflora	Rmu_sc0001023.1_g000022 Rmu_ssc0000111.1_g000011
rosa_roxburghii	Rroxscaffold_3G00270720 Rroxscaffold_6G00424050
rosa_rugosa	Rorug06G0453800
rosa_samantha	Rh3DG071900 Rh4BG110000 Rh4CG124300 Rh7AG056500 Rh7BG055800 Rh7CG057200 Rh7DG056100
rosa_wichuraiana	Rw7G004610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	215
AccII	CGCG	1 cut(s)	602
AciI	CCGC	6 cut(s)	183, 357, 458, 600, 602, 904
AcsI	RAATTY	3 cut(s)	206, 763, 832
AfaI	GTAC	2 cut(s)	1227, 1272
AfiI	CCNNNNNNNGG	2 cut(s)	900, 917
AgsI	TTSAA	5 cut(s)	433, 832, 837, 1041, 1285
AjnI	CCWGG	4 cut(s)	39, 197, 299, 689
AluBI	AGCT	7 cut(s)	643, 928, 1002, 1048, 1175, 1190, 1199
AluI	AGCT	7 cut(s)	643, 928, 1002, 1048, 1175, 1190, 1199
Alw21I	GWGCWC	2 cut(s)	279, 645
Alw26I	GTCTC	4 cut(s)	79, 137, 173, 1053
Ama87I	CYCGRG	2 cut(s)	548, 788
AoxI	GGCC	5 cut(s)	3, 620, 687, 692, 1147
ApeKI	GCWGC	2 cut(s)	88, 1094
ApoI	RAATTY	3 cut(s)	206, 763, 832
AspS9I	GGNCC	5 cut(s)	4, 97, 620, 693, 1029
AsuC2I	CCSGG	2 cut(s)	789, 790
AsuHPI	GGTGA	3 cut(s)	160, 740, 863
AsuII	TTCGAA	1 cut(s)	842
AvaI	CYCGRG	2 cut(s)	548, 788
AvaII	GGWCC	2 cut(s)	97, 1029
BaeGI	GKGCMC	1 cut(s)	220
BanI	GGYRCC	1 cut(s)	215
BanII	GRGCYC	1 cut(s)	645
Bbv12I	GWGCWC	2 cut(s)	279, 645
BbvI	GCAGC	2 cut(s)	75, 1106
BccI	CCATC	1 cut(s)	1145
BceAI	ACGGC	3 cut(s)	230, 607, 1262
BciT130I	CCWGG	4 cut(s)	41, 199, 301, 691
BclI	TGATCA	1 cut(s)	54
BcnI	CCSGG	2 cut(s)	789, 790
BcoDI	GTCTC	4 cut(s)	79, 137, 173, 1053
BfaI	CTAG	2 cut(s)	654, 1300
BfmI	CTRYAG	1 cut(s)	1072
BglI	GCCNNNNNGGC	1 cut(s)	619
BisI	GCNGC	3 cut(s)	89, 600, 1095
BlsI	GCNGC	3 cut(s)	90, 601, 1096
Bme1390I	CCNGG	6 cut(s)	41, 199, 301, 691, 789, 790
Bme18I	GGWCC	2 cut(s)	97, 1029
BmeT110I	CYCGRG	2 cut(s)	548, 788
BmgT120I	GGNCC	5 cut(s)	4, 97, 620, 693, 1029
BmiI	GGNNCC	3 cut(s)	217, 305, 1030
BmrFI	CCNGG	6 cut(s)	41, 199, 301, 691, 789, 790
BmsI	GCATC	2 cut(s)	284, 1026
Bpu14I	TTCGAA	1 cut(s)	842
BpuEI	CTTGAG	2 cut(s)	965, 1031
BpuMI	CCSGG	2 cut(s)	789, 790
BsaBI	GATNNNNATC	1 cut(s)	59
BsaI	GGTCTC	2 cut(s)	79, 173
BsaJI	CCNNGG	7 cut(s)	39, 40, 198, 579, 600, 788, 1150
BsaWI	WCCGGW	3 cut(s)	70, 1034, 1204
Bsc4I	CCNNNNNNNGG	2 cut(s)	900, 917
Bse1I	ACTGG	1 cut(s)	398
Bse3DI	GCAATG	3 cut(s)	165, 868, 1104
Bse8I	GATNNNNATC	1 cut(s)	59
BseBI	CCWGG	4 cut(s)	41, 199, 301, 691
BseDI	CCNNGG	7 cut(s)	39, 40, 198, 579, 600, 788, 1150
BseGI	GGATG	2 cut(s)	131, 664
BseJI	GATNNNNATC	1 cut(s)	59
BseLI	CCNNNNNNNGG	2 cut(s)	900, 917
BseMI	GCAATG	3 cut(s)	165, 868, 1104
BseMII	CTCAG	1 cut(s)	728
BseNI	ACTGG	1 cut(s)	398
BseRI	GAGGAG	3 cut(s)	184, 677, 748
BseSI	GKGCMC	1 cut(s)	220
BseXI	GCAGC	2 cut(s)	75, 1106
BseYI	CCCAGC	1 cut(s)	1048
Bsh1236I	CGCG	1 cut(s)	602
Bsh1285I	CGRYCG	1 cut(s)	258
BshFI	GGCC	5 cut(s)	5, 622, 689, 694, 1149
BshNI	GGYRCC	1 cut(s)	215
BsiEI	CGRYCG	1 cut(s)	258
BsiHKAI	GWGCWC	2 cut(s)	279, 645
BsiHKCI	CYCGRG	2 cut(s)	548, 788
BsiSI	CCGG	5 cut(s)	71, 671, 789, 1035, 1205
BslFI	GGGAC	1 cut(s)	215
BslI	CCNNNNNNNGG	2 cut(s)	900, 917
BsmAI	GTCTC	4 cut(s)	79, 137, 173, 1053
BsmBI	CGTCTC	1 cut(s)	137
BsmFI	GGGAC	1 cut(s)	215
BsmI	GAATGC	1 cut(s)	522
BsnI	GGCC	5 cut(s)	5, 622, 689, 694, 1149
Bso31I	GGTCTC	2 cut(s)	79, 173
BsoBI	CYCGRG	2 cut(s)	548, 788
Bsp119I	TTCGAA	1 cut(s)	842
Bsp1286I	GDGCHC	3 cut(s)	220, 279, 645
Bsp1407I	TGTACA	1 cut(s)	1270
Bsp143I	GATC	3 cut(s)	54, 592, 814
Bsp19I	CCATGG	1 cut(s)	579
BspACI	CCGC	6 cut(s)	183, 357, 458, 600, 602, 904
BspANI	GGCC	5 cut(s)	5, 622, 689, 694, 1149
BspCNI	CTCAG	1 cut(s)	727
BspFNI	CGCG	1 cut(s)	602
BspHI	TCATGA	1 cut(s)	706
BspLI	GGNNCC	3 cut(s)	217, 305, 1030
BspQI	GCTCTTC	1 cut(s)	1206
BspT104I	TTCGAA	1 cut(s)	842
BspT107I	GGYRCC	1 cut(s)	215
BspTNI	GGTCTC	2 cut(s)	79, 173
BsrDI	GCAATG	3 cut(s)	165, 868, 1104
BsrGI	TGTACA	1 cut(s)	1270
BsrI	ACTGG	1 cut(s)	398
BssECI	CCNNGG	7 cut(s)	39, 40, 198, 579, 600, 788, 1150
BssMI	GATC	3 cut(s)	54, 592, 814
BssT1I	CCWWGG	2 cut(s)	579, 1150
Bst2UI	CCWGG	4 cut(s)	41, 199, 301, 691
Bst4CI	ACNGT	6 cut(s)	112, 148, 256, 1076, 1117, 1170
Bst6I	CTCTTC	2 cut(s)	891, 1206
BstAUI	TGTACA	1 cut(s)	1270
BstBI	TTCGAA	1 cut(s)	842
BstC8I	GCNNGC	3 cut(s)	645, 974, 1197
BstDEI	CTNAG	1 cut(s)	714
BstDSI	CCRYGG	2 cut(s)	579, 600
BstF5I	GGATG	2 cut(s)	131, 664
BstFNI	CGCG	1 cut(s)	602
BstKTI	GATC	3 cut(s)	57, 595, 817
BstMAI	GTCTC	4 cut(s)	79, 137, 173, 1053
BstMBI	GATC	3 cut(s)	54, 592, 814
BstMCI	CGRYCG	1 cut(s)	258
BstMWI	GCNNNNNNNGC	3 cut(s)	167, 619, 1196
BstNI	CCWGG	4 cut(s)	41, 199, 301, 691
BstSCI	CCNGG	6 cut(s)	39, 197, 299, 689, 787, 788
BstSFI	CTRYAG	1 cut(s)	1072
BstSLI	GKGCMC	1 cut(s)	220
BstUI	CGCG	1 cut(s)	602
BstV1I	GCAGC	2 cut(s)	75, 1106
BsuRI	GGCC	5 cut(s)	5, 622, 689, 694, 1149
BtgI	CCRYGG	2 cut(s)	579, 600
BtsCI	GGATG	2 cut(s)	131, 664
BtsIMutI	CAGTG	1 cut(s)	1081
Cac8I	GCNNGC	3 cut(s)	645, 974, 1197
CciI	TCATGA	1 cut(s)	706
Cfr13I	GGNCC	5 cut(s)	4, 97, 620, 693, 1029
Cfr42I	CCGCGG	1 cut(s)	603
Cfr9I	CCCGGG	1 cut(s)	788
CseI	GACGC	2 cut(s)	134, 499
CsiI	ACCWGGT	1 cut(s)	299
Csp6I	GTAC	2 cut(s)	1226, 1271
CviAII	CATG	5 cut(s)	319, 352, 466, 580, 707
CviQI	GTAC	2 cut(s)	1226, 1271
DdeI	CTNAG	1 cut(s)	714
DpnI	GATC	3 cut(s)	56, 594, 816
DpnII	GATC	3 cut(s)	54, 592, 814
Eam1104I	CTCTTC	2 cut(s)	891, 1206
EarI	CTCTTC	2 cut(s)	891, 1206
EciI	GGCGGA	2 cut(s)	372, 919
Ecl136II	GAGCTC	1 cut(s)	643
Eco130I	CCWWGG	2 cut(s)	579, 1150
Eco24I	GRGCYC	1 cut(s)	645
Eco31I	GGTCTC	2 cut(s)	79, 173
Eco47I	GGWCC	2 cut(s)	97, 1029
Eco53kI	GAGCTC	1 cut(s)	643
Eco88I	CYCGRG	2 cut(s)	548, 788
EcoICRI	GAGCTC	1 cut(s)	643
EcoO109I	RGGNCCY	1 cut(s)	1029
EcoRI	GAATTC	2 cut(s)	763, 832
EcoRII	CCWGG	4 cut(s)	39, 197, 299, 689
EcoT14I	CCWWGG	2 cut(s)	579, 1150
EcoT38I	GRGCYC	1 cut(s)	645
ErhI	CCWWGG	2 cut(s)	579, 1150
Esp3I	CGTCTC	1 cut(s)	137
FaeI	CATG	5 cut(s)	322, 355, 469, 583, 710
FaqI	GGGAC	1 cut(s)	215
FatI	CATG	5 cut(s)	318, 351, 465, 579, 706
FbaI	TGATCA	1 cut(s)	54
Fnu4HI	GCNGC	3 cut(s)	89, 600, 1095
FokI	GGATG	2 cut(s)	118, 671
FriOI	GRGCYC	1 cut(s)	645
Fsp4HI	GCNGC	3 cut(s)	89, 600, 1095
FspBI	CTAG	2 cut(s)	654, 1300
GluI	GCNGC	3 cut(s)	89, 600, 1095
GsaI	CCCAGC	1 cut(s)	1052
HaeIII	GGCC	5 cut(s)	5, 622, 689, 694, 1149
HapII	CCGG	5 cut(s)	71, 671, 789, 1035, 1205
HgaI	GACGC	2 cut(s)	134, 499
Hin1II	CATG	5 cut(s)	322, 355, 469, 583, 710
HincII	GTYRAC	1 cut(s)	795
HindII	GTYRAC	1 cut(s)	795
HindIII	AAGCTT	1 cut(s)	1000
HpaII	CCGG	5 cut(s)	71, 671, 789, 1035, 1205
HphI	GGTGA	3 cut(s)	160, 740, 863
Hpy166II	GTNNAC	5 cut(s)	151, 537, 555, 682, 795
Hpy188I	TCNGA	7 cut(s)	59, 142, 190, 293, 1022, 1164, 1185
Hpy188III	TCNNGA	6 cut(s)	383, 487, 707, 911, 923, 1087
Hpy8I	GTNNAC	5 cut(s)	151, 537, 555, 682, 795
Hpy99I	CGWCG	4 cut(s)	142, 179, 251, 620
HpyAV	CCTTC	1 cut(s)	110
HpyCH4III	ACNGT	6 cut(s)	112, 148, 256, 1076, 1117, 1170
HpyCH4IV	ACGT	3 cut(s)	263, 413, 539
HpyCH4V	TGCA	1 cut(s)	297
HpyF10VI	GCNNNNNNNGC	3 cut(s)	167, 619, 1196
HpyF3I	CTNAG	1 cut(s)	714
HpySE526I	ACGT	3 cut(s)	263, 413, 539
Hsp92II	CATG	5 cut(s)	322, 355, 469, 583, 710
Ksp22I	TGATCA	1 cut(s)	54
KspI	CCGCGG	1 cut(s)	603
Kzo9I	GATC	3 cut(s)	54, 592, 814
LguI	GCTCTTC	1 cut(s)	1206
LmnI	GCTCC	2 cut(s)	282, 640
Lsp1109I	GCAGC	2 cut(s)	75, 1106
LweI	GCATC	2 cut(s)	284, 1026
MabI	ACCWGGT	1 cut(s)	299
MaeI	CTAG	2 cut(s)	654, 1300
MaeII	ACGT	3 cut(s)	263, 413, 539
MaeIII	GTNAC	6 cut(s)	106, 250, 259, 487, 697, 1156
MalI	GATC	3 cut(s)	56, 594, 816
MboI	GATC	3 cut(s)	54, 592, 814
MboII	GAAGA	5 cut(s)	59, 908, 992, 1060, 1193
MhlI	GDGCHC	3 cut(s)	220, 279, 645
MluCI	AATT	5 cut(s)	27, 206, 433, 763, 832
MlyI	GAGTC	1 cut(s)	185
MmeI	TCCRAC	1 cut(s)	120
MseI	TTAA	3 cut(s)	224, 390, 867
MspA1I	CMGCKG	1 cut(s)	602
MspI	CCGG	5 cut(s)	71, 671, 789, 1035, 1205
MspR9I	CCNGG	6 cut(s)	41, 199, 301, 691, 789, 790
Mva1269I	GAATGC	1 cut(s)	522
MvaI	CCWGG	4 cut(s)	41, 199, 301, 691
MvnI	CGCG	1 cut(s)	602
MwoI	GCNNNNNNNGC	3 cut(s)	167, 619, 1196
NciI	CCSGG	2 cut(s)	789, 790
NcoI	CCATGG	1 cut(s)	579
NdeII	GATC	3 cut(s)	54, 592, 814
NlaIII	CATG	5 cut(s)	322, 355, 469, 583, 710
NlaIV	GGNNCC	3 cut(s)	217, 305, 1030
NmeAIII	GCCGAG	1 cut(s)	1200
NmuCI	GTSAC	5 cut(s)	106, 250, 259, 487, 697
NspV	TTCGAA	1 cut(s)	842
PagI	TCATGA	1 cut(s)	706
PasI	CCCWGGG	1 cut(s)	40
PciSI	GCTCTTC	1 cut(s)	1206
PcsI	WCGNNNNNNNCGW	5 cut(s)	255, 260, 264, 476, 1263
PctI	GAATGC	1 cut(s)	522
PkrI	GCNGC	3 cut(s)	90, 601, 1096
PleI	GAGTC	1 cut(s)	185
PpsI	GAGTC	1 cut(s)	185
PpuMI	RGGWCCY	1 cut(s)	1029
Psp124BI	GAGCTC	1 cut(s)	645
Psp5II	RGGWCCY	1 cut(s)	1029
Psp6I	CCWGG	4 cut(s)	39, 197, 299, 689
PspFI	CCCAGC	1 cut(s)	1048
PspGI	CCWGG	4 cut(s)	39, 197, 299, 689
PspN4I	GGNNCC	3 cut(s)	217, 305, 1030
PspPI	GGNCC	5 cut(s)	4, 97, 620, 693, 1029
PspPPI	RGGWCCY	1 cut(s)	1029
PsrI	GAACNNNNNNTAC	2 cut(s)	945, 977
RsaI	GTAC	2 cut(s)	1227, 1272
RsaNI	GTAC	2 cut(s)	1226, 1271
SacI	GAGCTC	1 cut(s)	645
SacII	CCGCGG	1 cut(s)	603
SapI	GCTCTTC	1 cut(s)	1206
SaqAI	TTAA	3 cut(s)	224, 390, 867
SatI	GCNGC	3 cut(s)	89, 600, 1095
Sau3AI	GATC	3 cut(s)	54, 592, 814
Sau96I	GGNCC	5 cut(s)	4, 97, 620, 693, 1029
SchI	GAGTC	1 cut(s)	185
ScrFI	CCNGG	6 cut(s)	41, 199, 301, 691, 789, 790
SduI	GDGCHC	3 cut(s)	220, 279, 645
SexAI	ACCWGGT	1 cut(s)	299
SfaNI	GCATC	2 cut(s)	284, 1026
SfcI	CTRYAG	1 cut(s)	1072
Sfr303I	CCGCGG	1 cut(s)	603
SfuI	TTCGAA	1 cut(s)	842
SgrBI	CCGCGG	1 cut(s)	603
SinI	GGWCC	2 cut(s)	97, 1029
SmaI	CCCGGG	1 cut(s)	790
SmlI	CTYRAG	2 cut(s)	944, 1010
SmoI	CTYRAG	2 cut(s)	944, 1010
Sse9I	AATT	5 cut(s)	27, 206, 433, 763, 832
SsiI	CCGC	6 cut(s)	183, 357, 458, 600, 602, 904
SspMI	CTAG	2 cut(s)	654, 1300
SstI	GAGCTC	1 cut(s)	645
StyD4I	CCNGG	6 cut(s)	39, 197, 299, 689, 787, 788
StyI	CCWWGG	2 cut(s)	579, 1150
TaaI	ACNGT	6 cut(s)	112, 148, 256, 1076, 1117, 1170
TaiI	ACGT	3 cut(s)	266, 416, 542
TaqI	TCGA	8 cut(s)	402, 407, 470, 767, 813, 842, 878, 1257
TasI	AATT	5 cut(s)	27, 206, 433, 763, 832
TatI	WGTACW	1 cut(s)	1270
TauI	GCSGC	1 cut(s)	602
Tru1I	TTAA	3 cut(s)	224, 390, 867
Tru9I	TTAA	3 cut(s)	224, 390, 867
TscAI	CASTG	1 cut(s)	1081
TseFI	GTSAC	5 cut(s)	106, 250, 259, 487, 697
TseI	GCWGC	2 cut(s)	88, 1094
Tsp45I	GTSAC	5 cut(s)	106, 250, 259, 487, 697
TspDTI	ATGAA	4 cut(s)	120, 340, 454, 1054
TspGWI	ACGGA	1 cut(s)	67
TspMI	CCCGGG	1 cut(s)	788
TspRI	CASTG	1 cut(s)	1081
VpaK11BI	GGWCC	2 cut(s)	97, 1029
XapI	RAATTY	3 cut(s)	206, 763, 832
XmaI	CCCGGG	1 cut(s)	788
XspI	CTAG	2 cut(s)	654, 1300

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.