MD14G1212200.v1.1

subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0007322
Physical Location & Seq
Forward (+)
29714488 .. 29715835
1348 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1212200.v1.1.491

Sequence Viewer

Length: 1260 bp
ATGGCCCAAAACCCAAGACCCACCACAGTTCTCGAATCCCTGGGCGAAGAACTGATCAGAATCATCACTCCGGTCTCCGTCTGTATGCTGCTGGTGGTCCTTCTGGTCACTGTCCTCACCGACGACGCTTCATCCTCGTCGGAGAGGGTGACGTCAATCGCCACCATTGCGTACGACGAGACCGCCTCGGACTCCTCCTGGGACAAATTCGTAGGTGCCCTCTTAAACTCCCTCGTTTTTGTCGCCGTCGTCACTGCCGTCACATTCGTTTTGGTGCTCCTTTTTTACCTCAGATGCACCAGGTTCCTCAAAATCTACATGGGTTTTTCGTCTTTTGTTGTTCTGGGATTCATGGGCGGAGAAATCGCCCTGTTTCTGATTCAGGACTTTAATGTTCCGGTCGATTCGATTACGTTTGTTTTGGTGTTGTTCAATTTTGCTGTTGTTGGGGTTTTGGCGGTGTTCATGTCGAAAATGGCGATTTTCGTGACGCAGGGGTATTTGGTTGTGATTGGAATGCTGGTTGCTTATTGGTTTACGTTGTTGCCTGAGTGGACAACTTGGGTGCTGTTGGTTGCCATGGCATTGTATGATCTTGCCGCGGTTTTGTTGCCGGTCGGGCCGTTGAGGTTATTGGTGGAGCTGGCAATCTCTAGGGATGAGGAGATTCCGGCTCTCGTTTACGAGGCCAGGCCCGTGACTCCTCATGATTCTCAGGGTCCTGTGGGTGAGAGGAGGGTTTGGAGAGATAGGAGAAATGAGAATTCGAGTATTGGGAATGAGAATCCGAATTCAAATTCGAATCTTGGTGAGTTCGGCAATGTTGATAATGATTCGAGTTTGGTTAGAGCCGAAGAGGGGCGGATTCCAGAGAGGGATCAAGAGCTATCTGCACCATTACTCGAGCGTGTAGCGAATGTTCCGCCTTGCAGGCAGGAAGATGTTGCGGCGAGTGAAAGTTTGCTGCTTGAGGGCATCGGATTGGGGTCCTCCGGTTCAATCAAGCTGGGATTGGGAGACTTCATCTTCTACAGTGTTTTAGTCGGGAGGGCGGCAATGTATGATTTTATGACGGTGTATGCGTGTTATCTTGCCATCATAGGTGGCCTTGGAGTGACTCTGATACTGTTAGCTGTGTATCAGAAAGCTTTGCCAGCTCTTCCCGTGTCAATAATGCTCGGCGTACTGTTTTATGTATTGACACGGCTCTTACTCGAAGTTTTCGTTGTACAATGTTCTTTGAACCTCATCATGTTCTAG

Protein Analysis

420

Amino Acids

45.91

Weight (kDa)

4.54

Isoelectric Point (pI)

39.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Presenilin PF01080 14 - 409 1.1e-123 Presenilin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 155
AccB1I GGYRCC 1 cut(s) 215
AccII CGCG 1 cut(s) 602
AciI CCGC 9 cut(s) 183, 357, 458, 600, 602, 862, 923, 947, 1052
AclWI GGATC 1 cut(s) 885
AcsI RAATTY 4 cut(s) 206, 763, 790, 796
AcyI GRCGYC 1 cut(s) 152
AfaI GTAC 3 cut(s) 173, 1185, 1230
AfiI CCNNNNNNNGG 1 cut(s) 858
AgsI TTSAA 4 cut(s) 433, 795, 999, 1243
AjnI CCWGG 4 cut(s) 39, 197, 299, 689
AluBI AGCT 6 cut(s) 643, 886, 1006, 1133, 1148, 1157
AluI AGCT 6 cut(s) 643, 886, 1006, 1133, 1148, 1157
Alw21I GWGCWC 1 cut(s) 279
Alw26I GTCTC 3 cut(s) 79, 173, 1011
AlwI GGATC 1 cut(s) 885
Ama87I CYCGRG 1 cut(s) 902
AoxI GGCC 5 cut(s) 3, 620, 687, 692, 1105
ApeKI GCWGC 2 cut(s) 88, 964
ApoI RAATTY 4 cut(s) 206, 763, 790, 796
AspS9I GGNCC 6 cut(s) 4, 97, 620, 693, 719, 987
AsuHPI GGTGA 4 cut(s) 109, 160, 740, 821
AsuII TTCGAA 1 cut(s) 800
AvaI CYCGRG 1 cut(s) 902
AvaII GGWCC 3 cut(s) 97, 719, 987
BaeGI GKGCMC 1 cut(s) 220
BanI GGYRCC 1 cut(s) 215
Bbv12I GWGCWC 1 cut(s) 279
BbvI GCAGC 2 cut(s) 75, 951
BccI CCATC 1 cut(s) 1103
BceAI ACGGC 4 cut(s) 230, 242, 607, 1220
BciT130I CCWGG 4 cut(s) 41, 199, 301, 691
BclI TGATCA 1 cut(s) 54
BcoDI GTCTC 3 cut(s) 79, 173, 1011
BfaI CTAG 2 cut(s) 654, 1258
BfmI CTRYAG 1 cut(s) 1030
BglI GCCNNNNNGGC 2 cut(s) 619, 931
BisI GCNGC 5 cut(s) 89, 600, 948, 965, 1053
BlsI GCNGC 5 cut(s) 90, 601, 949, 966, 1054
Bme1390I CCNGG 4 cut(s) 41, 199, 301, 691
Bme18I GGWCC 3 cut(s) 97, 719, 987
BmeT110I CYCGRG 1 cut(s) 902
BmgT120I GGNCC 6 cut(s) 4, 97, 620, 693, 719, 987
BmiI GGNNCC 4 cut(s) 217, 305, 720, 988
BmrFI CCNGG 4 cut(s) 41, 199, 301, 691
BmsI GCATC 2 cut(s) 284, 984
BplI GAGNNNNNCTC 2 cut(s) 170, 202
Bpu14I TTCGAA 1 cut(s) 800
BpuEI CTTGAG 1 cut(s) 989
BsaBI GATNNNNATC 1 cut(s) 59
BsaHI GRCGYC 1 cut(s) 152
BsaI GGTCTC 2 cut(s) 79, 173
BsaJI CCNNGG 7 cut(s) 39, 40, 186, 198, 579, 600, 1108
BsaWI WCCGGW 3 cut(s) 70, 397, 992
Bsc4I CCNNNNNNNGG 1 cut(s) 858
Bse118I RCCGGY 1 cut(s) 613
Bse3DI GCAATG 3 cut(s) 165, 826, 1062
Bse8I GATNNNNATC 1 cut(s) 59
BseBI CCWGG 4 cut(s) 41, 199, 301, 691
BseDI CCNNGG 7 cut(s) 39, 40, 186, 198, 579, 600, 1108
BseGI GGATG 2 cut(s) 131, 664
BseJI GATNNNNATC 1 cut(s) 59
BseLI CCNNNNNNNGG 1 cut(s) 858
BseMI GCAATG 3 cut(s) 165, 826, 1062
BseMII CTCAG 3 cut(s) 304, 540, 728
BseRI GAGGAG 4 cut(s) 184, 677, 693, 748
BseSI GKGCMC 1 cut(s) 220
BseXI GCAGC 2 cut(s) 75, 951
BseYI CCCAGC 1 cut(s) 1006
BsgI GTGCAG 1 cut(s) 876
Bsh1236I CGCG 1 cut(s) 602
Bsh1285I CGRYCG 2 cut(s) 402, 618
BshFI GGCC 5 cut(s) 5, 622, 689, 694, 1107
BshNI GGYRCC 1 cut(s) 215
BsiEI CGRYCG 2 cut(s) 402, 618
BsiHKAI GWGCWC 1 cut(s) 279
BsiHKCI CYCGRG 1 cut(s) 902
BsiSI CCGG 5 cut(s) 71, 398, 614, 671, 993
BsiWI CGTACG 1 cut(s) 171
BslFI GGGAC 1 cut(s) 215
BslI CCNNNNNNNGG 1 cut(s) 858
BsmAI GTCTC 3 cut(s) 79, 173, 1011
BsmFI GGGAC 1 cut(s) 215
BsmI GAATGC 1 cut(s) 522
BsnI GGCC 5 cut(s) 5, 622, 689, 694, 1107
Bso31I GGTCTC 2 cut(s) 79, 173
BsoBI CYCGRG 1 cut(s) 902
Bsp119I TTCGAA 1 cut(s) 800
Bsp1286I GDGCHC 2 cut(s) 220, 279
Bsp1407I TGTACA 1 cut(s) 1228
Bsp143I GATC 3 cut(s) 54, 592, 877
Bsp19I CCATGG 1 cut(s) 579
BspACI CCGC 9 cut(s) 183, 357, 458, 600, 602, 862, 923, 947, 1052
BspANI GGCC 5 cut(s) 5, 622, 689, 694, 1107
BspCNI CTCAG 3 cut(s) 303, 541, 727
BspFNI CGCG 1 cut(s) 602
BspHI TCATGA 1 cut(s) 706
BspLI GGNNCC 4 cut(s) 217, 305, 720, 988
BspPI GGATC 1 cut(s) 885
BspQI GCTCTTC 1 cut(s) 1164
BspT104I TTCGAA 1 cut(s) 800
BspT107I GGYRCC 1 cut(s) 215
BspTNI GGTCTC 2 cut(s) 79, 173
BsrDI GCAATG 3 cut(s) 165, 826, 1062
BsrFI RCCGGY 1 cut(s) 613
BsrGI TGTACA 1 cut(s) 1228
BssAI RCCGGY 1 cut(s) 613
BssECI CCNNGG 7 cut(s) 39, 40, 186, 198, 579, 600, 1108
BssMI GATC 3 cut(s) 54, 592, 877
BssNI GRCGYC 1 cut(s) 152
BssT1I CCWWGG 2 cut(s) 579, 1108
Bst2UI CCWGG 4 cut(s) 41, 199, 301, 691
Bst4CI ACNGT 6 cut(s) 28, 112, 1034, 1075, 1128, 1188
Bst6I CTCTTC 2 cut(s) 849, 1164
BstACI GRCGYC 1 cut(s) 152
BstAUI TGTACA 1 cut(s) 1228
BstBI TTCGAA 1 cut(s) 800
BstC8I GCNNGC 3 cut(s) 645, 932, 1155
BstDEI CTNAG 3 cut(s) 290, 549, 714
BstDSI CCRYGG 2 cut(s) 579, 600
BstF5I GGATG 2 cut(s) 131, 664
BstFNI CGCG 1 cut(s) 602
BstKTI GATC 3 cut(s) 57, 595, 880
BstMAI GTCTC 3 cut(s) 79, 173, 1011
BstMBI GATC 3 cut(s) 54, 592, 877
BstMCI CGRYCG 2 cut(s) 402, 618
BstMWI GCNNNNNNNGC 4 cut(s) 167, 619, 931, 1154
BstNI CCWGG 4 cut(s) 41, 199, 301, 691
BstSCI CCNGG 4 cut(s) 39, 197, 299, 689
BstSFI CTRYAG 1 cut(s) 1030
BstSLI GKGCMC 1 cut(s) 220
BstUI CGCG 1 cut(s) 602
BstV1I GCAGC 2 cut(s) 75, 951
BsuRI GGCC 5 cut(s) 5, 622, 689, 694, 1107
BtgI CCRYGG 2 cut(s) 579, 600
BtsCI GGATG 2 cut(s) 131, 664
BtsI GCAGTG 1 cut(s) 252
BtsIMutI CAGTG 3 cut(s) 108, 252, 1039
Cac8I GCNNGC 3 cut(s) 645, 932, 1155
CciI TCATGA 1 cut(s) 706
Cfr10I RCCGGY 1 cut(s) 613
Cfr13I GGNCC 6 cut(s) 4, 97, 620, 693, 719, 987
Cfr42I CCGCGG 1 cut(s) 603
CseI GACGC 2 cut(s) 134, 499
CsiI ACCWGGT 1 cut(s) 299
Csp6I GTAC 3 cut(s) 172, 1184, 1229
CviAII CATG 6 cut(s) 319, 352, 466, 580, 707, 1252
CviQI GTAC 3 cut(s) 172, 1184, 1229
DdeI CTNAG 3 cut(s) 290, 549, 714
DpnI GATC 3 cut(s) 56, 594, 879
DpnII GATC 3 cut(s) 54, 592, 877
Eam1104I CTCTTC 2 cut(s) 849, 1164
EarI CTCTTC 2 cut(s) 849, 1164
EciI GGCGGA 3 cut(s) 372, 877, 912
Eco130I CCWWGG 2 cut(s) 579, 1108
Eco31I GGTCTC 2 cut(s) 79, 173
Eco47I GGWCC 3 cut(s) 97, 719, 987
Eco88I CYCGRG 1 cut(s) 902
EcoO109I RGGNCCY 2 cut(s) 719, 987
EcoRI GAATTC 2 cut(s) 763, 790
EcoRII CCWGG 4 cut(s) 39, 197, 299, 689
EcoT14I CCWWGG 2 cut(s) 579, 1108
ErhI CCWWGG 2 cut(s) 579, 1108
FaeI CATG 6 cut(s) 322, 355, 469, 583, 710, 1255
FaqI GGGAC 1 cut(s) 215
FatI CATG 6 cut(s) 318, 351, 465, 579, 706, 1251
FbaI TGATCA 1 cut(s) 54
Fnu4HI GCNGC 5 cut(s) 89, 600, 948, 965, 1053
FokI GGATG 2 cut(s) 118, 671
Fsp4HI GCNGC 5 cut(s) 89, 600, 948, 965, 1053
FspBI CTAG 2 cut(s) 654, 1258
GluI GCNGC 5 cut(s) 89, 600, 948, 965, 1053
GsaI CCCAGC 1 cut(s) 1010
HaeIII GGCC 5 cut(s) 5, 622, 689, 694, 1107
HapII CCGG 5 cut(s) 71, 398, 614, 671, 993
HgaI GACGC 2 cut(s) 134, 499
Hin1I GRCGYC 1 cut(s) 152
Hin1II CATG 6 cut(s) 322, 355, 469, 583, 710, 1255
HindIII AAGCTT 1 cut(s) 1146
HpaII CCGG 5 cut(s) 71, 398, 614, 671, 993
HphI GGTGA 4 cut(s) 109, 160, 740, 821
Hpy166II GTNNAC 3 cut(s) 537, 555, 682
Hpy188I TCNGA 9 cut(s) 59, 142, 190, 293, 378, 789, 980, 1122, 1143
Hpy188III TCNNGA 7 cut(s) 32, 383, 487, 707, 869, 881, 1045
Hpy8I GTNNAC 3 cut(s) 537, 555, 682
Hpy99I CGWCG 5 cut(s) 125, 128, 142, 179, 251
HpyAV CCTTC 1 cut(s) 110
HpyCH4III ACNGT 6 cut(s) 28, 112, 1034, 1075, 1128, 1188
HpyCH4IV ACGT 3 cut(s) 152, 413, 539
HpyCH4V TGCA 3 cut(s) 297, 893, 930
HpyF10VI GCNNNNNNNGC 4 cut(s) 167, 619, 931, 1154
HpyF3I CTNAG 3 cut(s) 290, 549, 714
HpySE526I ACGT 3 cut(s) 152, 413, 539
Hsp92I GRCGYC 1 cut(s) 152
Hsp92II CATG 6 cut(s) 322, 355, 469, 583, 710, 1255
Ksp22I TGATCA 1 cut(s) 54
KspI CCGCGG 1 cut(s) 603
Kzo9I GATC 3 cut(s) 54, 592, 877
LguI GCTCTTC 1 cut(s) 1164
LmnI GCTCC 2 cut(s) 282, 640
Lsp1109I GCAGC 2 cut(s) 75, 951
LweI GCATC 2 cut(s) 284, 984
MabI ACCWGGT 1 cut(s) 299
MaeI CTAG 2 cut(s) 654, 1258
MaeII ACGT 3 cut(s) 152, 413, 539
MaeIII GTNAC 7 cut(s) 106, 148, 250, 259, 487, 697, 1114
MalI GATC 3 cut(s) 56, 594, 879
MboI GATC 3 cut(s) 54, 592, 877
MboII GAAGA 5 cut(s) 59, 866, 950, 1018, 1151
MhlI GDGCHC 2 cut(s) 220, 279
MluCI AATT 5 cut(s) 206, 433, 763, 790, 796
MlyI GAGTC 3 cut(s) 185, 694, 1111
MmeI TCCRAC 1 cut(s) 120
MseI TTAA 2 cut(s) 224, 390
MspA1I CMGCKG 1 cut(s) 602
MspI CCGG 5 cut(s) 71, 398, 614, 671, 993
MspR9I CCNGG 4 cut(s) 41, 199, 301, 691
Mva1269I GAATGC 1 cut(s) 522
MvaI CCWGG 4 cut(s) 41, 199, 301, 691
MvnI CGCG 1 cut(s) 602
MwoI GCNNNNNNNGC 4 cut(s) 167, 619, 931, 1154
NcoI CCATGG 1 cut(s) 579
NdeII GATC 3 cut(s) 54, 592, 877
NlaIII CATG 6 cut(s) 322, 355, 469, 583, 710, 1255
NlaIV GGNNCC 4 cut(s) 217, 305, 720, 988
NmeAIII GCCGAG 1 cut(s) 1158
NmuCI GTSAC 7 cut(s) 106, 148, 250, 259, 487, 697, 1114
NspV TTCGAA 1 cut(s) 800
PaeR7I CTCGAG 1 cut(s) 902
PagI TCATGA 1 cut(s) 706
PasI CCCWGGG 1 cut(s) 40
PciSI GCTCTTC 1 cut(s) 1164
PcsI WCGNNNNNNNCGW 4 cut(s) 255, 476, 1079, 1221
PctI GAATGC 1 cut(s) 522
Pfl23II CGTACG 1 cut(s) 171
PkrI GCNGC 5 cut(s) 90, 601, 949, 966, 1054
PleI GAGTC 3 cut(s) 185, 694, 1111
PpsI GAGTC 3 cut(s) 185, 694, 1111
PpuMI RGGWCCY 2 cut(s) 719, 987
Psp5II RGGWCCY 2 cut(s) 719, 987
Psp6I CCWGG 4 cut(s) 39, 197, 299, 689
PspFI CCCAGC 1 cut(s) 1006
PspGI CCWGG 4 cut(s) 39, 197, 299, 689
PspLI CGTACG 1 cut(s) 171
PspN4I GGNNCC 4 cut(s) 217, 305, 720, 988
PspPI GGNCC 6 cut(s) 4, 97, 620, 693, 719, 987
PspPPI RGGWCCY 2 cut(s) 719, 987
PspXI VCTCGAGB 1 cut(s) 902
PsrI GAACNNNNNNTAC 2 cut(s) 903, 935
RsaI GTAC 3 cut(s) 173, 1185, 1230
RsaNI GTAC 3 cut(s) 172, 1184, 1229
SacII CCGCGG 1 cut(s) 603
SapI GCTCTTC 1 cut(s) 1164
SaqAI TTAA 2 cut(s) 224, 390
SatI GCNGC 5 cut(s) 89, 600, 948, 965, 1053
Sau3AI GATC 3 cut(s) 54, 592, 877
Sau96I GGNCC 6 cut(s) 4, 97, 620, 693, 719, 987
SchI GAGTC 3 cut(s) 185, 694, 1111
ScrFI CCNGG 4 cut(s) 41, 199, 301, 691
SduI GDGCHC 2 cut(s) 220, 279
SexAI ACCWGGT 1 cut(s) 299
SfaNI GCATC 2 cut(s) 284, 984
SfcI CTRYAG 1 cut(s) 1030
Sfr274I CTCGAG 1 cut(s) 902
Sfr303I CCGCGG 1 cut(s) 603
SfuI TTCGAA 1 cut(s) 800
SgrBI CCGCGG 1 cut(s) 603
SinI GGWCC 3 cut(s) 97, 719, 987
SlaI CTCGAG 1 cut(s) 902
SmlI CTYRAG 2 cut(s) 902, 968
SmoI CTYRAG 2 cut(s) 902, 968
Sse9I AATT 5 cut(s) 206, 433, 763, 790, 796
SsiI CCGC 9 cut(s) 183, 357, 458, 600, 602, 862, 923, 947, 1052
SspMI CTAG 2 cut(s) 654, 1258
StyD4I CCNGG 4 cut(s) 39, 197, 299, 689
StyI CCWWGG 2 cut(s) 579, 1108
TaaI ACNGT 6 cut(s) 28, 112, 1034, 1075, 1128, 1188
TaiI ACGT 3 cut(s) 155, 416, 542
TaqI TCGA 9 cut(s) 33, 402, 407, 470, 767, 800, 836, 903, 1215
TasI AATT 5 cut(s) 206, 433, 763, 790, 796
TatI WGTACW 1 cut(s) 1228
TauI GCSGC 3 cut(s) 602, 950, 1055
Tru1I TTAA 2 cut(s) 224, 390
Tru9I TTAA 2 cut(s) 224, 390
TscAI CASTG 3 cut(s) 115, 259, 1039
TseFI GTSAC 7 cut(s) 106, 148, 250, 259, 487, 697, 1114
TseI GCWGC 2 cut(s) 88, 964
Tsp45I GTSAC 7 cut(s) 106, 148, 250, 259, 487, 697, 1114
TspDTI ATGAA 4 cut(s) 120, 340, 454, 1012
TspGWI ACGGA 1 cut(s) 67
TspRI CASTG 3 cut(s) 115, 259, 1039
VpaK11BI GGWCC 3 cut(s) 97, 719, 987
XapI RAATTY 4 cut(s) 206, 763, 790, 796
XhoI CTCGAG 1 cut(s) 902
XspI CTAG 2 cut(s) 654, 1258
ZraI GACGTC 1 cut(s) 153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.