pycom14g17580

subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0007322
Physical Location & Seq
Forward (+)
19454334 .. 19455323
990 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g17580.1

Sequence Viewer

Length: 900 bp
ATGTCATCGTTTAAAAAAAGGTCGTGCTATTCATACGTTTATGTTTATTCTCATACCTACAAAATTAATGGGCAAAAATTAACAATATTGTATATGAAATTGTTTCCCCAACGCCGTCGACCAGTCCAAGCAGTTCTTTCACCAAAACACCTGCGAAACAGTTATCAGCCTCGACCAAATCTCAAATCCGAAATCCCAAAATCCGCCCCAAGATTCGAAATTGATCCGATTGTCGAACACCCAATGGCCCAAAACCCAAGACCCACAACAGTTCTCGAATCCCTGGGCGAAGAACTGATCAGAATCATCACTCCGGTCTCCGTCTGTATGCTGCTGGTGGTCCTTCTGGTCACTGTCCTCATCGACGACGCTTCATCCTCGTCGGAGACGGTGACCTCAATCGCCACCATTGCGTACGACGAGACTGTCTCGGACTCCTCCTGGGACAAATTCGTAGGTGCCCTCTTAAACTCCCTCGTTTTTGTCGTCGTCGTCACTGTCGTCACGTTCGTTCTGGTGCTCCTTTTTTACCTGAGATGCACCAGGTTCCTCAAAATCTACATGGGTTTTTCGTCTTTTGTTGTTCTGGGTTTCATGGGCGGAGAAATCGCCCTTTTTTTGATTCAGGACTTTAATGTTCCGGTCGATTCGATTACGTTTGTTTTGGTGTTGTTCAATTTTGCTGTTGTTGGGGTTTTGGCGGTGTTCATGTCGAAAATGGCGATTTTCGTGACGCAGGGGTATTTGGTTGTGATTGGAATGCTGGTTGCTTATTGGTTTACGTTGTTGCCTGAGTGGACAACTTGGGTGCTGTTGGTTGCCATGGCATTGTATCGATCTTGCCGCGGTTTTGTTGCCGGTTGGGCCGTTGAGGTTATTGGTGGAGCTGGCAATCTCTAG

Protein Analysis

300

Amino Acids

33.53

Weight (kDa)

8.97

Isoelectric Point (pI)

43.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Presenilin PF01080 95 - 278 2.2e-54 Presenilin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 159
AasI GACNNNNNNGTC 1 cut(s) 425
Acc36I ACCTGC 1 cut(s) 159
AccB1I GGYRCC 1 cut(s) 458
AccI GTMKAC 1 cut(s) 118
AccII CGCG 1 cut(s) 846
AciI CCGC 5 cut(s) 204, 600, 701, 844, 846
AclWI GGATC 1 cut(s) 218
AcsI RAATTY 1 cut(s) 449
AfaI GTAC 1 cut(s) 416
AgsI TTSAA 1 cut(s) 676
AjnI CCWGG 3 cut(s) 282, 440, 542
AluBI AGCT 1 cut(s) 887
AluI AGCT 1 cut(s) 887
Alw21I GWGCWC 1 cut(s) 522
Alw26I GTCTC 4 cut(s) 322, 380, 416, 433
AlwI GGATC 1 cut(s) 218
AoxI GGCC 2 cut(s) 246, 864
ApeKI GCWGC 1 cut(s) 331
ApoI RAATTY 1 cut(s) 449
AseI ATTAAT 1 cut(s) 66
AspS9I GGNCC 3 cut(s) 247, 340, 864
AsuHPI GGTGA 2 cut(s) 132, 403
AsuII TTCGAA 1 cut(s) 216
AvaII GGWCC 1 cut(s) 340
BaeGI GKGCMC 1 cut(s) 463
BanI GGYRCC 1 cut(s) 458
Bbv12I GWGCWC 1 cut(s) 522
BbvI GCAGC 1 cut(s) 318
BceAI ACGGC 2 cut(s) 99, 851
BciT130I CCWGG 3 cut(s) 284, 442, 544
BclI TGATCA 1 cut(s) 297
BcoDI GTCTC 4 cut(s) 322, 380, 416, 433
BfaI CTAG 1 cut(s) 898
BfuAI ACCTGC 1 cut(s) 159
BglI GCCNNNNNGGC 1 cut(s) 863
BisI GCNGC 2 cut(s) 332, 844
BlsI GCNGC 2 cut(s) 333, 845
Bme1390I CCNGG 3 cut(s) 284, 442, 544
Bme18I GGWCC 1 cut(s) 340
BmgT120I GGNCC 3 cut(s) 247, 340, 864
BmiI GGNNCC 2 cut(s) 460, 548
BmrFI CCNGG 3 cut(s) 284, 442, 544
BmsI GCATC 1 cut(s) 527
BplI GAGNNNNNCTC 2 cut(s) 413, 445
Bpu14I TTCGAA 1 cut(s) 216
Bsa29I ATCGAT 1 cut(s) 835
BsaBI GATNNNNATC 1 cut(s) 302
BsaI GGTCTC 1 cut(s) 322
BsaJI CCNNGG 5 cut(s) 282, 283, 441, 822, 844
BsaWI WCCGGW 2 cut(s) 313, 640
Bse118I RCCGGY 1 cut(s) 857
Bse1I ACTGG 1 cut(s) 122
Bse3DI GCAATG 1 cut(s) 408
Bse8I GATNNNNATC 1 cut(s) 302
BseBI CCWGG 3 cut(s) 284, 442, 544
BseCI ATCGAT 1 cut(s) 835
BseDI CCNNGG 5 cut(s) 282, 283, 441, 822, 844
BseGI GGATG 1 cut(s) 374
BseJI GATNNNNATC 1 cut(s) 302
BseMI GCAATG 1 cut(s) 408
BseMII CTCAG 2 cut(s) 524, 783
BseNI ACTGG 1 cut(s) 122
BseRI GAGGAG 1 cut(s) 427
BseSI GKGCMC 1 cut(s) 463
BseXI GCAGC 1 cut(s) 318
Bsh1236I CGCG 1 cut(s) 846
Bsh1285I CGRYCG 1 cut(s) 645
BshFI GGCC 2 cut(s) 248, 866
BshNI GGYRCC 1 cut(s) 458
BshVI ATCGAT 1 cut(s) 835
BsiEI CGRYCG 1 cut(s) 645
BsiHKAI GWGCWC 1 cut(s) 522
BsiSI CCGG 3 cut(s) 314, 641, 858
BsiWI CGTACG 1 cut(s) 414
BslFI GGGAC 1 cut(s) 458
BsmAI GTCTC 4 cut(s) 322, 380, 416, 433
BsmBI CGTCTC 1 cut(s) 380
BsmFI GGGAC 1 cut(s) 458
BsmI GAATGC 1 cut(s) 765
BsnI GGCC 2 cut(s) 248, 866
Bso31I GGTCTC 1 cut(s) 322
Bsp119I TTCGAA 1 cut(s) 216
Bsp1286I GDGCHC 2 cut(s) 463, 522
Bsp143I GATC 3 cut(s) 223, 297, 836
Bsp19I CCATGG 1 cut(s) 822
BspACI CCGC 5 cut(s) 204, 600, 701, 844, 846
BspANI GGCC 2 cut(s) 248, 866
BspCNI CTCAG 2 cut(s) 525, 784
BspDI ATCGAT 1 cut(s) 835
BspFNI CGCG 1 cut(s) 846
BspLI GGNNCC 2 cut(s) 460, 548
BspMI ACCTGC 1 cut(s) 159
BspPI GGATC 1 cut(s) 218
BspT104I TTCGAA 1 cut(s) 216
BspT107I GGYRCC 1 cut(s) 458
BspTNI GGTCTC 1 cut(s) 322
BsrDI GCAATG 1 cut(s) 408
BsrFI RCCGGY 1 cut(s) 857
BsrI ACTGG 1 cut(s) 122
BssAI RCCGGY 1 cut(s) 857
BssECI CCNNGG 5 cut(s) 282, 283, 441, 822, 844
BssMI GATC 3 cut(s) 223, 297, 836
BssT1I CCWWGG 1 cut(s) 822
Bst2UI CCWGG 3 cut(s) 284, 442, 544
Bst4CI ACNGT 6 cut(s) 161, 271, 355, 391, 427, 499
BstBI TTCGAA 1 cut(s) 216
BstC8I GCNNGC 1 cut(s) 889
BstDEI CTNAG 2 cut(s) 533, 792
BstDSI CCRYGG 2 cut(s) 822, 844
BstEII GGTNACC 1 cut(s) 391
BstF5I GGATG 1 cut(s) 374
BstFNI CGCG 1 cut(s) 846
BstKTI GATC 3 cut(s) 226, 300, 839
BstMAI GTCTC 4 cut(s) 322, 380, 416, 433
BstMBI GATC 3 cut(s) 223, 297, 836
BstMCI CGRYCG 1 cut(s) 645
BstMWI GCNNNNNNNGC 2 cut(s) 410, 863
BstNI CCWGG 3 cut(s) 284, 442, 544
BstPI GGTNACC 1 cut(s) 391
BstSCI CCNGG 3 cut(s) 282, 440, 542
BstSLI GKGCMC 1 cut(s) 463
BstUI CGCG 1 cut(s) 846
BstV1I GCAGC 1 cut(s) 318
Bsu15I ATCGAT 1 cut(s) 835
BsuRI GGCC 2 cut(s) 248, 866
BsuTUI ATCGAT 1 cut(s) 835
BtgI CCRYGG 2 cut(s) 822, 844
BtsCI GGATG 1 cut(s) 374
BtsIMutI CAGTG 2 cut(s) 351, 495
BveI ACCTGC 1 cut(s) 159
Cac8I GCNNGC 1 cut(s) 889
Cfr10I RCCGGY 1 cut(s) 857
Cfr13I GGNCC 3 cut(s) 247, 340, 864
Cfr42I CCGCGG 1 cut(s) 847
ClaI ATCGAT 1 cut(s) 835
CseI GACGC 2 cut(s) 377, 742
CsiI ACCWGGT 1 cut(s) 542
Csp6I GTAC 1 cut(s) 415
CviAII CATG 4 cut(s) 562, 595, 709, 823
CviJI RGCY 4 cut(s) 169, 248, 866, 887
CviKI_1 RGCY 4 cut(s) 169, 248, 866, 887
CviQI GTAC 1 cut(s) 415
DdeI CTNAG 2 cut(s) 533, 792
DpnI GATC 3 cut(s) 225, 299, 838
DpnII GATC 3 cut(s) 223, 297, 836
DraI TTTAAA 1 cut(s) 13
DrdI GACNNNNNNGTC 1 cut(s) 425
DseDI GACNNNNNNGTC 1 cut(s) 425
EciI GGCGGA 2 cut(s) 193, 615
Eco130I CCWWGG 1 cut(s) 822
Eco31I GGTCTC 1 cut(s) 322
Eco47I GGWCC 1 cut(s) 340
Eco91I GGTNACC 1 cut(s) 391
EcoO65I GGTNACC 1 cut(s) 391
EcoRII CCWGG 3 cut(s) 282, 440, 542
EcoT14I CCWWGG 1 cut(s) 822
ErhI CCWWGG 1 cut(s) 822
Esp3I CGTCTC 1 cut(s) 380
FaeI CATG 4 cut(s) 565, 598, 712, 826
FalI AAGNNNNNCTT 2 cut(s) 120, 152
FaqI GGGAC 1 cut(s) 458
FatI CATG 4 cut(s) 561, 594, 708, 822
FbaI TGATCA 1 cut(s) 297
FblI GTMKAC 1 cut(s) 118
Fnu4HI GCNGC 2 cut(s) 332, 844
FokI GGATG 1 cut(s) 361
Fsp4HI GCNGC 2 cut(s) 332, 844
FspBI CTAG 1 cut(s) 898
GluI GCNGC 2 cut(s) 332, 844
HaeIII GGCC 2 cut(s) 248, 866
HapII CCGG 3 cut(s) 314, 641, 858
HgaI GACGC 2 cut(s) 377, 742
Hin1II CATG 4 cut(s) 565, 598, 712, 826
HincII GTYRAC 1 cut(s) 119
HindII GTYRAC 1 cut(s) 119
HinfI GANTC 6 cut(s) 213, 278, 303, 434, 622, 647
HpaII CCGG 3 cut(s) 314, 641, 858
HphI GGTGA 2 cut(s) 132, 403
Hpy166II GTNNAC 3 cut(s) 119, 780, 798
Hpy188I TCNGA 5 cut(s) 190, 228, 302, 385, 433
Hpy188III TCNNGA 3 cut(s) 275, 626, 730
Hpy8I GTNNAC 3 cut(s) 119, 780, 798
Hpy99I CGWCG 7 cut(s) 120, 368, 371, 385, 422, 491, 494
HpyAV CCTTC 1 cut(s) 353
HpyCH4III ACNGT 6 cut(s) 161, 271, 355, 391, 427, 499
HpyCH4IV ACGT 4 cut(s) 36, 506, 656, 782
HpyCH4V TGCA 1 cut(s) 540
HpyF10VI GCNNNNNNNGC 2 cut(s) 410, 863
HpyF3I CTNAG 2 cut(s) 533, 792
HpySE526I ACGT 4 cut(s) 36, 506, 656, 782
Hsp92II CATG 4 cut(s) 565, 598, 712, 826
Ksp22I TGATCA 1 cut(s) 297
KspI CCGCGG 1 cut(s) 847
Kzo9I GATC 3 cut(s) 223, 297, 836
LmnI GCTCC 2 cut(s) 525, 884
Lsp1109I GCAGC 1 cut(s) 318
LweI GCATC 1 cut(s) 527
MabI ACCWGGT 1 cut(s) 542
MaeI CTAG 1 cut(s) 898
MaeII ACGT 4 cut(s) 36, 506, 656, 782
MaeIII GTNAC 5 cut(s) 349, 391, 493, 502, 730
MalI GATC 3 cut(s) 225, 299, 838
MboI GATC 3 cut(s) 223, 297, 836
MboII GAAGA 1 cut(s) 302
MhlI GDGCHC 2 cut(s) 463, 522
MluCI AATT 6 cut(s) 63, 77, 98, 219, 449, 676
MlyI GAGTC 1 cut(s) 428
MmeI TCCRAC 1 cut(s) 363
MnlI CCTC 9 cut(s) 180, 368, 388, 406, 448, 473, 485, 560, 865
MseI TTAA 5 cut(s) 12, 66, 80, 467, 633
MspA1I CMGCKG 1 cut(s) 846
MspI CCGG 3 cut(s) 314, 641, 858
MspR9I CCNGG 3 cut(s) 284, 442, 544
Mva1269I GAATGC 1 cut(s) 765
MvaI CCWGG 3 cut(s) 284, 442, 544
MvnI CGCG 1 cut(s) 846
MwoI GCNNNNNNNGC 2 cut(s) 410, 863
NcoI CCATGG 1 cut(s) 822
NdeII GATC 3 cut(s) 223, 297, 836
NlaIII CATG 4 cut(s) 565, 598, 712, 826
NlaIV GGNNCC 2 cut(s) 460, 548
NmuCI GTSAC 5 cut(s) 349, 391, 493, 502, 730
NspV TTCGAA 1 cut(s) 216
PaqCI CACCTGC 1 cut(s) 159
PasI CCCWGGG 1 cut(s) 283
PcsI WCGNNNNNNNCGW 4 cut(s) 483, 498, 507, 719
PctI GAATGC 1 cut(s) 765
PfeI GAWTC 5 cut(s) 213, 278, 303, 622, 647
Pfl23II CGTACG 1 cut(s) 414
PkrI GCNGC 2 cut(s) 333, 845
PleI GAGTC 1 cut(s) 428
PpsI GAGTC 1 cut(s) 428
PshBI ATTAAT 1 cut(s) 66
Psp6I CCWGG 3 cut(s) 282, 440, 542
PspEI GGTNACC 1 cut(s) 391
PspGI CCWGG 3 cut(s) 282, 440, 542
PspLI CGTACG 1 cut(s) 414
PspN4I GGNNCC 2 cut(s) 460, 548
PspPI GGNCC 3 cut(s) 247, 340, 864
RsaI GTAC 1 cut(s) 416
RsaNI GTAC 1 cut(s) 415
SacII CCGCGG 1 cut(s) 847
SalI GTCGAC 1 cut(s) 117
SaqAI TTAA 5 cut(s) 12, 66, 80, 467, 633
SatI GCNGC 2 cut(s) 332, 844
Sau3AI GATC 3 cut(s) 223, 297, 836
Sau96I GGNCC 3 cut(s) 247, 340, 864
SchI GAGTC 1 cut(s) 428
ScrFI CCNGG 3 cut(s) 284, 442, 544
SduI GDGCHC 2 cut(s) 463, 522
SexAI ACCWGGT 1 cut(s) 542
SfaNI GCATC 1 cut(s) 527
Sfr303I CCGCGG 1 cut(s) 847
SfuI TTCGAA 1 cut(s) 216
SgrBI CCGCGG 1 cut(s) 847
SinI GGWCC 1 cut(s) 340
Sse9I AATT 6 cut(s) 63, 77, 98, 219, 449, 676
SsiI CCGC 5 cut(s) 204, 600, 701, 844, 846
SspI AATATT 1 cut(s) 87
SspMI CTAG 1 cut(s) 898
StyD4I CCNGG 3 cut(s) 282, 440, 542
StyI CCWWGG 1 cut(s) 822
TaaI ACNGT 6 cut(s) 161, 271, 355, 391, 427, 499
TaiI ACGT 4 cut(s) 39, 509, 659, 785
TasI AATT 6 cut(s) 63, 77, 98, 219, 449, 676
TauI GCSGC 1 cut(s) 846
TfiI GAWTC 5 cut(s) 213, 278, 303, 622, 647
Tru1I TTAA 5 cut(s) 12, 66, 80, 467, 633
Tru9I TTAA 5 cut(s) 12, 66, 80, 467, 633
TscAI CASTG 2 cut(s) 358, 502
TseFI GTSAC 5 cut(s) 349, 391, 493, 502, 730
TseI GCWGC 1 cut(s) 331
Tsp45I GTSAC 5 cut(s) 349, 391, 493, 502, 730
TspDTI ATGAA 5 cut(s) 21, 110, 363, 583, 697
TspGWI ACGGA 1 cut(s) 310
TspRI CASTG 2 cut(s) 358, 502
VpaK11BI GGWCC 1 cut(s) 340
VspI ATTAAT 1 cut(s) 66
XapI RAATTY 1 cut(s) 449
XmiI GTMKAC 1 cut(s) 118
XspI CTAG 1 cut(s) 898
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.