MD06G1227800.v1.1

Converts alpha-aldose to the beta-anomer

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0011418
Physical Location & Seq
Reverse (-)
35815524 .. 35817763
2240 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1227800.v1.1.491

Sequence Viewer

Length: 1020 bp
ATGGCGGATGCGAACCAGAAACCAGAAATCTTCGAACTGAACAATGGGAGCATGCGCGCCCTCATCACAAACTTAGGCTGCACCATCACCTCCTTGTCTGTTCCCGGCAAAGACGGAAAATTGGCTGATGTTGTTCTTGGATTCGACTCCGTGGATCCCTATGTGAAAGGAGTTGCTCCTTACTTTGGCAGCATTGTGGGTCGGGTTGCTAATAGAATCAAAGACGGGAAGTTTACGCTCAATGGAACGGAGTACTCCTTGCCTGTCAACAAACCTCCGAACAGTCTCCATGGTGGTCACAGGGGGTATGATAAGCAGATATGGGAGGTAGCAGAACTTAAAAAGGGCGATAACCCGTCCATCACTTTTAAGTATCACAGTCATGATGGTGAGGAAGGTTACCCCGGGAATCTGTCGCTGACTGCAACTTATACACTTACTTCAAGCACGACAATGAGACTTGACATGGAAGCAGTGCCTGAGAACAAGCCCACCCCAGTCAGCTTAGCTCAGCACACCTACTGGAACTTAGCTGGCCATAACTCGGGCAACATACTTGACCATGCAATTCAAATTTGGGCAAACCATATTACCCCTGTGGATGAAAACACCATCCCAACAGGTGAAATCAAGCCGGTTAAAGGGACCCCCTTTGATTTCACTGCCGAGAAGAGGGTCGGTGAGTCCATCCATGAGGTCGGTTTAGGGTATGACCACAACTACGTGCTCGACTGTGGGGAAGAGAAAGAAGGCCTGAAACATGCTGCAAGAGTAAGGGACCCATCCAGCTCGAGGGTCCTTAACCTATGGAGCAATGCCCCTGGGATGCAGTTTTACACCGCGAATTATGTCAATGGGGTTGTCGGCAAAGGAGGCGCTGTCTATGGAAAGCATTCCGGGCTCTGTTTGGAGACGCAGGGGTTCCCAAATGCAATTAACACACCAAACTTCCCGTCCATTGTTGTTCAACCCGGTGAGAGGTACAGCCACACCATGTTGTTTGAGTTTTCAGTTGAGTGA

Protein Analysis

340

Amino Acids

36.98

Weight (kDa)

5.9

Isoelectric Point (pI)

24.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldose_epim PF01263 11 - 334 2.4e-98 Aldose 1-epimerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011418)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17940
fragaria_vesca FvH4_5g17440 FvH4_5g17440 FvH4_5g17440
malus_domestica MD06G1227800.v1.1 MD14G1236900.v1.1
prunus_persica Prupe.5G234200_v2.0.a1
pyrus_communis pycom06g20410 pycom14g19790
rosa_chinensis RchiOBHm_Chr7g0178251
rosa_laevigata RLG00000005459
rosa_multiflora Rmu_co8199974.1_g000001
rosa_roxburghii Rroxscaffold_3G00275040
rosa_rugosa Rorug06G0415500 Rorug06G0415600
rosa_samantha Rh7AG014600 Rh7BG014000 Rh7CG013900 Rh7DG014200
rosa_wichuraiana Rw7G001140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 57, 842
AciI CCGC 2 cut(s) 5, 840
AclWI GGATC 2 cut(s) 149, 162
AcoI YGGCCR 1 cut(s) 535
AcsI RAATTY 1 cut(s) 573
AfaI GTAC 2 cut(s) 254, 983
AfiI CCNNNNNNNGG 6 cut(s) 185, 544, 641, 672, 792, 978
AgsI TTSAA 3 cut(s) 444, 572, 968
AjnI CCWGG 1 cut(s) 820
AluBI AGCT 4 cut(s) 504, 509, 533, 789
AluI AGCT 4 cut(s) 504, 509, 533, 789
Alw21I GWGCWC 1 cut(s) 729
Alw26I GTCTC 3 cut(s) 290, 451, 905
AlwI GGATC 2 cut(s) 149, 162
AlwNI CAGNNNCTG 1 cut(s) 479
Ama87I CYCGRG 3 cut(s) 404, 544, 790
AoxI GGCC 2 cut(s) 535, 751
ApeKI GCWGC 3 cut(s) 78, 189, 764
ApoI RAATTY 1 cut(s) 573
ArsI GACNNNNNNTTYG 2 cut(s) 938, 970
Asp700I GAANNNNTTC 1 cut(s) 892
AspLEI GCGC 3 cut(s) 57, 59, 878
AspS9I GGNCC 3 cut(s) 645, 778, 796
AsuC2I CCSGG 5 cut(s) 105, 405, 406, 898, 972
AsuHPI GGTGA 5 cut(s) 79, 401, 635, 692, 986
AsuII TTCGAA 1 cut(s) 33
AvaI CYCGRG 3 cut(s) 404, 544, 790
AvaII GGWCC 3 cut(s) 645, 778, 796
BalI TGGCCA 1 cut(s) 537
BamHI GGATCC 1 cut(s) 154
BanII GRGCYC 1 cut(s) 903
Bbv12I GWGCWC 1 cut(s) 729
BbvI GCAGC 3 cut(s) 65, 201, 751
BccI CCATC 6 cut(s) 92, 368, 380, 620, 695, 790
BciT130I CCWGG 1 cut(s) 822
BcnI CCSGG 5 cut(s) 105, 405, 406, 898, 972
BcoDI GTCTC 3 cut(s) 290, 451, 905
BfoI RGCGCY 1 cut(s) 879
BisI GCNGC 3 cut(s) 79, 190, 765
BlpI GCTNAGC 2 cut(s) 505, 510
BlsI GCNGC 3 cut(s) 80, 191, 766
BmcAI AGTACT 1 cut(s) 254
Bme1390I CCNGG 6 cut(s) 105, 405, 406, 822, 898, 972
Bme18I GGWCC 3 cut(s) 645, 778, 796
BmeT110I CYCGRG 3 cut(s) 404, 544, 790
BmgT120I GGNCC 3 cut(s) 645, 778, 796
BmiI GGNNCC 7 cut(s) 156, 646, 647, 779, 780, 797, 923
BmrFI CCNGG 6 cut(s) 105, 405, 406, 822, 898, 972
BmrI ACTGGG 1 cut(s) 491
BmsI GCATC 1 cut(s) 816
BmuI ACTGGG 1 cut(s) 491
Bpu1102I GCTNAGC 2 cut(s) 505, 510
Bpu14I TTCGAA 1 cut(s) 33
BpuMI CCSGG 5 cut(s) 105, 405, 406, 898, 972
BsaAI YACGTR 1 cut(s) 724
BsaJI CCNNGG 6 cut(s) 150, 289, 403, 404, 820, 821
BsaXI ACNNNNNCTCC 2 cut(s) 864, 894
Bsc4I CCNNNNNNNGG 6 cut(s) 185, 544, 641, 672, 792, 978
Bse118I RCCGGY 1 cut(s) 634
Bse1I ACTGG 2 cut(s) 497, 527
Bse3DI GCAATG 1 cut(s) 820
BseBI CCWGG 1 cut(s) 822
BseDI CCNNGG 6 cut(s) 150, 289, 403, 404, 820, 821
BseGI GGATG 6 cut(s) 13, 607, 612, 687, 782, 831
BseLI CCNNNNNNNGG 6 cut(s) 185, 544, 641, 672, 792, 978
BseMI GCAATG 1 cut(s) 820
BseMII CTCAG 2 cut(s) 471, 524
BseNI ACTGG 2 cut(s) 497, 527
BsePI GCGCGC 1 cut(s) 55
BseXI GCAGC 3 cut(s) 65, 201, 751
BsgI GTGCAG 1 cut(s) 64
Bsh1236I CGCG 2 cut(s) 57, 842
BshFI GGCC 2 cut(s) 537, 753
BsiHKAI GWGCWC 1 cut(s) 729
BsiHKCI CYCGRG 3 cut(s) 404, 544, 790
BsiSI CCGG 5 cut(s) 105, 405, 635, 897, 972
BslFI GGGAC 2 cut(s) 658, 791
BslI CCNNNNNNNGG 6 cut(s) 185, 544, 641, 672, 792, 978
BsmAI GTCTC 3 cut(s) 290, 451, 905
BsmBI CGTCTC 1 cut(s) 905
BsmFI GGGAC 2 cut(s) 658, 791
BsmI GAATGC 1 cut(s) 892
BsnI GGCC 2 cut(s) 537, 753
BsoBI CYCGRG 3 cut(s) 404, 544, 790
Bsp119I TTCGAA 1 cut(s) 33
Bsp1286I GDGCHC 2 cut(s) 729, 903
Bsp143I GATC 1 cut(s) 154
Bsp1720I GCTNAGC 2 cut(s) 505, 510
Bsp19I CCATGG 1 cut(s) 289
BspACI CCGC 2 cut(s) 5, 840
BspANI GGCC 2 cut(s) 537, 753
BspCNI CTCAG 2 cut(s) 472, 523
BspFNI CGCG 2 cut(s) 57, 842
BspHI TCATGA 1 cut(s) 382
BspLI GGNNCC 7 cut(s) 156, 646, 647, 779, 780, 797, 923
BspPI GGATC 2 cut(s) 149, 162
BspT104I TTCGAA 1 cut(s) 33
BsrDI GCAATG 1 cut(s) 820
BsrFI RCCGGY 1 cut(s) 634
BsrI ACTGG 2 cut(s) 497, 527
BssAI RCCGGY 1 cut(s) 634
BssECI CCNNGG 6 cut(s) 150, 289, 403, 404, 820, 821
BssHII GCGCGC 1 cut(s) 55
BssMI GATC 1 cut(s) 154
BssT1I CCWWGG 1 cut(s) 289
Bst2UI CCWGG 1 cut(s) 822
Bst4CI ACNGT 3 cut(s) 284, 380, 734
Bst6I CTCTTC 2 cut(s) 665, 735
BstBAI YACGTR 1 cut(s) 724
BstBI TTCGAA 1 cut(s) 33
BstC8I GCNNGC 3 cut(s) 53, 57, 535
BstDEI CTNAG 5 cut(s) 73, 480, 505, 510, 529
BstDSI CCRYGG 2 cut(s) 150, 289
BstEII GGTNACC 1 cut(s) 398
BstF5I GGATG 6 cut(s) 13, 607, 612, 687, 782, 831
BstFNI CGCG 2 cut(s) 57, 842
BstH2I RGCGCY 1 cut(s) 879
BstHHI GCGC 3 cut(s) 57, 59, 878
BstKTI GATC 1 cut(s) 157
BstMAI GTCTC 3 cut(s) 290, 451, 905
BstMBI GATC 1 cut(s) 154
BstMWI GCNNNNNNNGC 2 cut(s) 873, 898
BstNI CCWGG 1 cut(s) 822
BstNSI RCATGY 2 cut(s) 55, 764
BstPI GGTNACC 1 cut(s) 398
BstSCI CCNGG 6 cut(s) 103, 403, 404, 820, 896, 970
BstUI CGCG 2 cut(s) 57, 842
BstV1I GCAGC 3 cut(s) 65, 201, 751
BstX2I RGATCY 1 cut(s) 154
BstYI RGATCY 1 cut(s) 154
BsuRI GGCC 2 cut(s) 537, 753
BtgI CCRYGG 2 cut(s) 150, 289
BtsCI GGATG 6 cut(s) 13, 607, 612, 687, 782, 831
BtsI GCAGTG 2 cut(s) 480, 660
BtsIMutI CAGTG 2 cut(s) 480, 660
Cac8I GCNNGC 3 cut(s) 53, 57, 535
CaiI CAGNNNCTG 1 cut(s) 479
CciI TCATGA 1 cut(s) 382
CfoI GCGC 3 cut(s) 57, 59, 878
Cfr10I RCCGGY 1 cut(s) 634
Cfr13I GGNCC 3 cut(s) 645, 778, 796
Cfr9I CCCGGG 1 cut(s) 404
CseI GACGC 1 cut(s) 922
Csp6I GTAC 2 cut(s) 253, 982
CviAII CATG 8 cut(s) 52, 290, 383, 466, 563, 692, 761, 994
CviQI GTAC 2 cut(s) 253, 982
DdeI CTNAG 5 cut(s) 73, 480, 505, 510, 529
DpnI GATC 1 cut(s) 156
DpnII GATC 1 cut(s) 154
EaeI YGGCCR 1 cut(s) 535
Eam1104I CTCTTC 2 cut(s) 665, 735
EarI CTCTTC 2 cut(s) 665, 735
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 1 cut(s) 289
Eco147I AGGCCT 1 cut(s) 753
Eco24I GRGCYC 1 cut(s) 903
Eco47I GGWCC 3 cut(s) 645, 778, 796
Eco88I CYCGRG 3 cut(s) 404, 544, 790
Eco91I GGTNACC 1 cut(s) 398
EcoO109I RGGNCCY 3 cut(s) 645, 778, 796
EcoO65I GGTNACC 1 cut(s) 398
EcoRII CCWGG 1 cut(s) 820
EcoT14I CCWWGG 1 cut(s) 289
EcoT38I GRGCYC 1 cut(s) 903
ErhI CCWWGG 1 cut(s) 289
Esp3I CGTCTC 1 cut(s) 905
FaeI CATG 8 cut(s) 55, 293, 386, 469, 566, 695, 764, 997
FaqI GGGAC 2 cut(s) 658, 791
FatI CATG 8 cut(s) 51, 289, 382, 465, 562, 691, 760, 993
Fnu4HI GCNGC 3 cut(s) 79, 190, 765
FokI GGATG 6 cut(s) 20, 599, 614, 674, 769, 838
FriOI GRGCYC 1 cut(s) 903
Fsp4HI GCNGC 3 cut(s) 79, 190, 765
GlaI GCGC 3 cut(s) 56, 58, 877
GluI GCNGC 3 cut(s) 79, 190, 765
HaeII RGCGCY 1 cut(s) 879
HaeIII GGCC 2 cut(s) 537, 753
HapII CCGG 5 cut(s) 105, 405, 635, 897, 972
HgaI GACGC 1 cut(s) 922
HhaI GCGC 3 cut(s) 57, 59, 878
Hin1II CATG 8 cut(s) 55, 293, 386, 469, 566, 695, 764, 997
Hin6I GCGC 3 cut(s) 55, 57, 876
HinP1I GCGC 3 cut(s) 55, 57, 876
HincII GTYRAC 1 cut(s) 268
HindII GTYRAC 1 cut(s) 268
HinfI GANTC 5 cut(s) 141, 146, 216, 409, 683
HpaII CCGG 5 cut(s) 105, 405, 635, 897, 972
HphI GGTGA 5 cut(s) 79, 401, 635, 692, 986
Hpy166II GTNNAC 2 cut(s) 234, 268
Hpy188I TCNGA 1 cut(s) 279
Hpy188III TCNNGA 1 cut(s) 383
Hpy8I GTNNAC 2 cut(s) 234, 268
HpyAV CCTTC 2 cut(s) 389, 743
HpyCH4III ACNGT 3 cut(s) 284, 380, 734
HpyCH4IV ACGT 1 cut(s) 723
HpyCH4V TGCA 6 cut(s) 81, 425, 566, 767, 829, 932
HpyF10VI GCNNNNNNNGC 2 cut(s) 873, 898
HpyF3I CTNAG 5 cut(s) 73, 480, 505, 510, 529
HpySE526I ACGT 1 cut(s) 723
Hsp92II CATG 8 cut(s) 55, 293, 386, 469, 566, 695, 764, 997
HspAI GCGC 3 cut(s) 55, 57, 876
KflI GGGWCCC 2 cut(s) 645, 778
Kzo9I GATC 1 cut(s) 154
LmnI GCTCC 3 cut(s) 48, 181, 810
Lsp1109I GCAGC 3 cut(s) 65, 201, 751
LweI GCATC 1 cut(s) 816
MaeII ACGT 1 cut(s) 723
MaeIII GTNAC 2 cut(s) 296, 398
MalI GATC 1 cut(s) 156
MboI GATC 1 cut(s) 154
MboII GAAGA 3 cut(s) 22, 682, 752
MflI RGATCY 1 cut(s) 154
MhlI GDGCHC 2 cut(s) 729, 903
MlsI TGGCCA 1 cut(s) 537
MluCI AATT 5 cut(s) 119, 567, 573, 844, 933
MluNI TGGCCA 1 cut(s) 537
MlyI GAGTC 2 cut(s) 140, 692
Mox20I TGGCCA 1 cut(s) 537
MroXI GAANNNNTTC 1 cut(s) 892
MscI TGGCCA 1 cut(s) 537
MseI TTAA 5 cut(s) 339, 369, 639, 801, 936
MslI CAYNNNNRTG 3 cut(s) 381, 387, 452
Msp20I TGGCCA 1 cut(s) 537
MspI CCGG 5 cut(s) 105, 405, 635, 897, 972
MspR9I CCNGG 6 cut(s) 105, 405, 406, 822, 898, 972
Mva1269I GAATGC 1 cut(s) 892
MvaI CCWGG 1 cut(s) 822
MvnI CGCG 2 cut(s) 57, 842
MwoI GCNNNNNNNGC 2 cut(s) 873, 898
NciI CCSGG 5 cut(s) 105, 405, 406, 898, 972
NcoI CCATGG 1 cut(s) 289
NdeII GATC 1 cut(s) 154
NlaIII CATG 8 cut(s) 55, 293, 386, 469, 566, 695, 764, 997
NlaIV GGNNCC 7 cut(s) 156, 646, 647, 779, 780, 797, 923
NmeAIII GCCGAG 1 cut(s) 691
NmuCI GTSAC 1 cut(s) 296
NspI RCATGY 2 cut(s) 55, 764
NspV TTCGAA 1 cut(s) 33
PaeI GCATGC 1 cut(s) 55
PaeR7I CTCGAG 1 cut(s) 790
PagI TCATGA 1 cut(s) 382
PasI CCCWGGG 1 cut(s) 821
PauI GCGCGC 1 cut(s) 55
PceI AGGCCT 1 cut(s) 753
PctI GAATGC 1 cut(s) 892
PdmI GAANNNNTTC 1 cut(s) 892
PfeI GAWTC 3 cut(s) 141, 216, 409
PkrI GCNGC 3 cut(s) 80, 191, 766
PleI GAGTC 2 cut(s) 140, 691
PpsI GAGTC 2 cut(s) 140, 691
Ppu21I YACGTR 1 cut(s) 724
PpuMI RGGWCCY 3 cut(s) 645, 778, 796
Psp5II RGGWCCY 3 cut(s) 645, 778, 796
Psp6I CCWGG 1 cut(s) 820
PspEI GGTNACC 1 cut(s) 398
PspGI CCWGG 1 cut(s) 820
PspN4I GGNNCC 7 cut(s) 156, 646, 647, 779, 780, 797, 923
PspPI GGNCC 3 cut(s) 645, 778, 796
PspPPI RGGWCCY 3 cut(s) 645, 778, 796
PspXI VCTCGAGB 1 cut(s) 790
PstNI CAGNNNCTG 1 cut(s) 479
PsuI RGATCY 1 cut(s) 154
PteI GCGCGC 1 cut(s) 55
RsaI GTAC 2 cut(s) 254, 983
RsaNI GTAC 2 cut(s) 253, 982
RseI CAYNNNNRTG 3 cut(s) 381, 387, 452
SaqAI TTAA 5 cut(s) 339, 369, 639, 801, 936
SatI GCNGC 3 cut(s) 79, 190, 765
Sau3AI GATC 1 cut(s) 154
Sau96I GGNCC 3 cut(s) 645, 778, 796
ScaI AGTACT 1 cut(s) 254
SchI GAGTC 2 cut(s) 140, 692
ScrFI CCNGG 6 cut(s) 105, 405, 406, 822, 898, 972
SduI GDGCHC 2 cut(s) 729, 903
SfaNI GCATC 1 cut(s) 816
Sfr274I CTCGAG 1 cut(s) 790
SfuI TTCGAA 1 cut(s) 33
SinI GGWCC 3 cut(s) 645, 778, 796
SlaI CTCGAG 1 cut(s) 790
SmaI CCCGGG 1 cut(s) 406
SmiMI CAYNNNNRTG 3 cut(s) 381, 387, 452
SmlI CTYRAG 1 cut(s) 790
SmoI CTYRAG 1 cut(s) 790
SphI GCATGC 1 cut(s) 55
Sse9I AATT 5 cut(s) 119, 567, 573, 844, 933
SseBI AGGCCT 1 cut(s) 753
SsiI CCGC 2 cut(s) 5, 840
StuI AGGCCT 1 cut(s) 753
StyD4I CCNGG 6 cut(s) 103, 403, 404, 820, 896, 970
StyI CCWWGG 1 cut(s) 289
TaaI ACNGT 3 cut(s) 284, 380, 734
TaiI ACGT 1 cut(s) 726
TaqI TCGA 4 cut(s) 33, 144, 729, 791
TasI AATT 5 cut(s) 119, 567, 573, 844, 933
TatI WGTACW 1 cut(s) 252
TfiI GAWTC 3 cut(s) 141, 216, 409
Tru1I TTAA 5 cut(s) 339, 369, 639, 801, 936
Tru9I TTAA 5 cut(s) 339, 369, 639, 801, 936
TscAI CASTG 2 cut(s) 480, 667
TseFI GTSAC 1 cut(s) 296
TseI GCWGC 3 cut(s) 78, 189, 764
Tsp45I GTSAC 1 cut(s) 296
TspDTI ATGAA 1 cut(s) 618
TspGWI ACGGA 3 cut(s) 129, 139, 263
TspMI CCCGGG 1 cut(s) 404
TspRI CASTG 2 cut(s) 480, 667
VpaK11BI GGWCC 3 cut(s) 645, 778, 796
XapI RAATTY 1 cut(s) 573
XceI RCATGY 2 cut(s) 55, 764
XhoI CTCGAG 1 cut(s) 790
XmaI CCCGGG 1 cut(s) 404
XmnI GAANNNNTTC 1 cut(s) 892
ZrmI AGTACT 1 cut(s) 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.