MD14G1236900.v1.1

Converts alpha-aldose to the beta-anomer

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0011418
Physical Location & Seq
Reverse (-)
31670770 .. 31673030
2261 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1236900.v1.1.491

Sequence Viewer

Length: 1020 bp
ATGGCGGATGCGAACCAGAATCCAGAAATCTTTGAGCTCAACAATGGGAGCATGCGCGCCCTCATCACAAACTTTGGCTGCACCATCACTTCCTTGTCTGTTCCCGGAAAAGACGGAAAATTGGCTGATGTTGTTCTTGGATTCGACTCCGTGGATCCCTATGTGAAAGGACTTGCTCCTTACTTTGGCTGCATTGTGGGTCGGGTTGCTAATCGAATCAAAGATGGGAAGTTTAAGCTCAATGGAACGGAGTACTCCTTGCCTATCAACAGACCTCCGAACAGTCTCCATGGTGGTCACAAGGGGTACGATAAGCAGATATGGAAGGTAGCAGAACATAAAAAGGGCGATAACCCGTCCATCACTTTTAAGTATCACAGTCATGATGGGGAGGAAGGTTACCCCGGGAATCTTTCACTGACTGCAACTTATACGCTTACTTCAAGCACGACGATGAGACTTGACATGGAAGCAGTGCCTGAGAACAAGCCCACCCCGGTCAGCCTGGCTCAGCACACATACTGGAACTTAGCTGGACATAACTCGGGCAACATACTTGACCATGCAATTCAAATTCGGGCAAACCATGTTACCCCTGTGGATGAAAACACAGTCCCAACAGGTGAAATCAAGCCGGTTAAAGGCACGCCCTTTGATTTCACTGCCGAGAAGAGGGTCGGTGAGTCCATCCATGAGGTCGGTTTAGGGTATGACCACAACTATGTGCTCGACTGTGGGGAAGAGAAACAAGGCCTGAAACATGCTGCAAGAGTGAAGGACCCATCCAGCTCGAGGGTCCTTAACCTATGGAGCAATGCCCCCGGGATGCAGTTTTACACCGCGAATTATGTCAATGGGGTTGTCGGCAAAGGAGGCGCTGTCTATGGAAAGCATGCCGGGCTCTGTCTGGAGACACAAGGGTTCCCGAATGCAATTAACACACCGAACTTCCCGTCTATTGTTGTTCAACCCGGTGAGAAGTACAGCCACACCATGTTGTTTGAGTTTTCAGTTGAGTGA

Protein Analysis

340

Amino Acids

36.96

Weight (kDa)

6.39

Isoelectric Point (pI)

24.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldose_epim PF01263 11 - 334 6.5e-98 Aldose 1-epimerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011418)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17940
fragaria_vesca FvH4_5g17440 FvH4_5g17440 FvH4_5g17440
malus_domestica MD06G1227800.v1.1 MD14G1236900.v1.1
prunus_persica Prupe.5G234200_v2.0.a1
pyrus_communis pycom06g20410 pycom14g19790
rosa_chinensis RchiOBHm_Chr7g0178251
rosa_laevigata RLG00000005459
rosa_multiflora Rmu_co8199974.1_g000001
rosa_roxburghii Rroxscaffold_3G00275040
rosa_rugosa Rorug06G0415500 Rorug06G0415600
rosa_samantha Rh7AG014600 Rh7BG014000 Rh7CG013900 Rh7DG014200
rosa_wichuraiana Rw7G001140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 57, 842
AciI CCGC 2 cut(s) 5, 840
AclWI GGATC 2 cut(s) 149, 162
AcsI RAATTY 1 cut(s) 573
AfaI GTAC 3 cut(s) 254, 308, 983
AfiI CCNNNNNNNGG 4 cut(s) 185, 641, 672, 792
AgsI TTSAA 3 cut(s) 444, 572, 968
AjnI CCWGG 1 cut(s) 504
AluBI AGCT 4 cut(s) 37, 238, 533, 789
AluI AGCT 4 cut(s) 37, 238, 533, 789
Alw21I GWGCWC 2 cut(s) 39, 729
Alw26I GTCTC 3 cut(s) 290, 451, 905
AlwI GGATC 2 cut(s) 149, 162
AlwNI CAGNNNCTG 1 cut(s) 479
Ama87I CYCGRG 4 cut(s) 404, 544, 790, 821
AoxI GGCC 1 cut(s) 751
ApeKI GCWGC 3 cut(s) 78, 189, 764
ApoI RAATTY 1 cut(s) 573
ArsI GACNNNNNNTTYG 2 cut(s) 938, 970
AspLEI GCGC 3 cut(s) 57, 59, 878
AspS9I GGNCC 2 cut(s) 778, 796
AsuC2I CCSGG 8 cut(s) 105, 405, 406, 497, 822, 823, 898, 972
AsuHPI GGTGA 3 cut(s) 635, 692, 986
AvaI CYCGRG 4 cut(s) 404, 544, 790, 821
AvaII GGWCC 2 cut(s) 778, 796
BamHI GGATCC 1 cut(s) 154
BanII GRGCYC 2 cut(s) 39, 903
Bbv12I GWGCWC 2 cut(s) 39, 729
BbvI GCAGC 3 cut(s) 65, 176, 751
BccI CCATC 6 cut(s) 92, 218, 368, 380, 695, 790
BciT130I CCWGG 1 cut(s) 506
BcnI CCSGG 8 cut(s) 105, 405, 406, 497, 822, 823, 898, 972
BcoDI GTCTC 3 cut(s) 290, 451, 905
BfoI RGCGCY 1 cut(s) 879
BisI GCNGC 3 cut(s) 79, 190, 765
BlpI GCTNAGC 1 cut(s) 510
BlsI GCNGC 3 cut(s) 80, 191, 766
BmcAI AGTACT 1 cut(s) 254
Bme1390I CCNGG 9 cut(s) 105, 405, 406, 497, 506, 822, 823, 898, 972
Bme18I GGWCC 2 cut(s) 778, 796
BmeT110I CYCGRG 4 cut(s) 404, 544, 790, 821
BmgT120I GGNCC 2 cut(s) 778, 796
BmiI GGNNCC 4 cut(s) 156, 780, 797, 923
BmrFI CCNGG 9 cut(s) 105, 405, 406, 497, 506, 822, 823, 898, 972
BmsI GCATC 1 cut(s) 816
BpmI CTGGAG 1 cut(s) 929
Bpu1102I GCTNAGC 1 cut(s) 510
BpuMI CCSGG 8 cut(s) 105, 405, 406, 497, 822, 823, 898, 972
BsaJI CCNNGG 7 cut(s) 150, 289, 403, 404, 495, 820, 821
BsaXI ACNNNNNCTCC 2 cut(s) 864, 894
Bsc4I CCNNNNNNNGG 4 cut(s) 185, 641, 672, 792
Bse118I RCCGGY 1 cut(s) 634
Bse1I ACTGG 1 cut(s) 527
Bse3DI GCAATG 1 cut(s) 820
BseBI CCWGG 1 cut(s) 506
BseDI CCNNGG 7 cut(s) 150, 289, 403, 404, 495, 820, 821
BseGI GGATG 5 cut(s) 13, 607, 687, 782, 831
BseLI CCNNNNNNNGG 4 cut(s) 185, 641, 672, 792
BseMI GCAATG 1 cut(s) 820
BseMII CTCAG 2 cut(s) 471, 524
BseNI ACTGG 1 cut(s) 527
BsePI GCGCGC 1 cut(s) 55
BseXI GCAGC 3 cut(s) 65, 176, 751
BsgI GTGCAG 1 cut(s) 64
Bsh1236I CGCG 2 cut(s) 57, 842
BshFI GGCC 1 cut(s) 753
BsiHKAI GWGCWC 2 cut(s) 39, 729
BsiHKCI CYCGRG 4 cut(s) 404, 544, 790, 821
BsiSI CCGG 7 cut(s) 105, 405, 497, 635, 822, 897, 972
BslFI GGGAC 1 cut(s) 599
BslI CCNNNNNNNGG 4 cut(s) 185, 641, 672, 792
BsmAI GTCTC 3 cut(s) 290, 451, 905
BsmFI GGGAC 1 cut(s) 599
BsmI GAATGC 1 cut(s) 934
BsnI GGCC 1 cut(s) 753
BsoBI CYCGRG 4 cut(s) 404, 544, 790, 821
Bsp1286I GDGCHC 3 cut(s) 39, 729, 903
Bsp143I GATC 1 cut(s) 154
Bsp1720I GCTNAGC 1 cut(s) 510
Bsp19I CCATGG 1 cut(s) 289
BspACI CCGC 2 cut(s) 5, 840
BspANI GGCC 1 cut(s) 753
BspCNI CTCAG 2 cut(s) 472, 523
BspFNI CGCG 2 cut(s) 57, 842
BspHI TCATGA 1 cut(s) 382
BspLI GGNNCC 4 cut(s) 156, 780, 797, 923
BspPI GGATC 2 cut(s) 149, 162
BsrDI GCAATG 1 cut(s) 820
BsrFI RCCGGY 1 cut(s) 634
BsrI ACTGG 1 cut(s) 527
BssAI RCCGGY 1 cut(s) 634
BssECI CCNNGG 7 cut(s) 150, 289, 403, 404, 495, 820, 821
BssHII GCGCGC 1 cut(s) 55
BssMI GATC 1 cut(s) 154
BssT1I CCWWGG 1 cut(s) 289
Bst2UI CCWGG 1 cut(s) 506
Bst4CI ACNGT 4 cut(s) 284, 380, 613, 734
Bst6I CTCTTC 2 cut(s) 665, 735
BstC8I GCNNGC 4 cut(s) 53, 57, 647, 894
BstDEI CTNAG 3 cut(s) 480, 510, 529
BstDSI CCRYGG 2 cut(s) 150, 289
BstEII GGTNACC 1 cut(s) 398
BstF5I GGATG 5 cut(s) 13, 607, 687, 782, 831
BstFNI CGCG 2 cut(s) 57, 842
BstH2I RGCGCY 1 cut(s) 879
BstHHI GCGC 3 cut(s) 57, 59, 878
BstKTI GATC 1 cut(s) 157
BstMAI GTCTC 3 cut(s) 290, 451, 905
BstMBI GATC 1 cut(s) 154
BstMWI GCNNNNNNNGC 2 cut(s) 873, 898
BstNI CCWGG 1 cut(s) 506
BstNSI RCATGY 3 cut(s) 55, 764, 896
BstPI GGTNACC 1 cut(s) 398
BstSCI CCNGG 9 cut(s) 103, 403, 404, 495, 504, 820, 821, 896, 970
BstUI CGCG 2 cut(s) 57, 842
BstV1I GCAGC 3 cut(s) 65, 176, 751
BstX2I RGATCY 1 cut(s) 154
BstYI RGATCY 1 cut(s) 154
BsuRI GGCC 1 cut(s) 753
BtgI CCRYGG 2 cut(s) 150, 289
BtsCI GGATG 5 cut(s) 13, 607, 687, 782, 831
BtsI GCAGTG 2 cut(s) 480, 660
BtsIMutI CAGTG 3 cut(s) 416, 480, 660
Cac8I GCNNGC 4 cut(s) 53, 57, 647, 894
CaiI CAGNNNCTG 1 cut(s) 479
CciI TCATGA 1 cut(s) 382
CfoI GCGC 3 cut(s) 57, 59, 878
Cfr10I RCCGGY 1 cut(s) 634
Cfr13I GGNCC 2 cut(s) 778, 796
Cfr9I CCCGGG 2 cut(s) 404, 821
Csp6I GTAC 3 cut(s) 253, 307, 982
CviQI GTAC 3 cut(s) 253, 307, 982
DdeI CTNAG 3 cut(s) 480, 510, 529
DpnI GATC 1 cut(s) 156
DpnII GATC 1 cut(s) 154
Eam1104I CTCTTC 2 cut(s) 665, 735
EarI CTCTTC 2 cut(s) 665, 735
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 37
Eco130I CCWWGG 1 cut(s) 289
Eco147I AGGCCT 1 cut(s) 753
Eco24I GRGCYC 2 cut(s) 39, 903
Eco47I GGWCC 2 cut(s) 778, 796
Eco53kI GAGCTC 1 cut(s) 37
Eco88I CYCGRG 4 cut(s) 404, 544, 790, 821
Eco91I GGTNACC 1 cut(s) 398
EcoICRI GAGCTC 1 cut(s) 37
EcoO109I RGGNCCY 2 cut(s) 778, 796
EcoO65I GGTNACC 1 cut(s) 398
EcoRII CCWGG 1 cut(s) 504
EcoT14I CCWWGG 1 cut(s) 289
EcoT38I GRGCYC 2 cut(s) 39, 903
ErhI CCWWGG 1 cut(s) 289
FaqI GGGAC 1 cut(s) 599
Fnu4HI GCNGC 3 cut(s) 79, 190, 765
FokI GGATG 5 cut(s) 20, 614, 674, 769, 838
FriOI GRGCYC 2 cut(s) 39, 903
Fsp4HI GCNGC 3 cut(s) 79, 190, 765
GlaI GCGC 3 cut(s) 56, 58, 877
GluI GCNGC 3 cut(s) 79, 190, 765
GsuI CTGGAG 1 cut(s) 929
HaeII RGCGCY 1 cut(s) 879
HaeIII GGCC 1 cut(s) 753
HapII CCGG 7 cut(s) 105, 405, 497, 635, 822, 897, 972
HhaI GCGC 3 cut(s) 57, 59, 878
Hin6I GCGC 3 cut(s) 55, 57, 876
HinP1I GCGC 3 cut(s) 55, 57, 876
HinfI GANTC 6 cut(s) 19, 141, 146, 216, 409, 683
HpaII CCGG 7 cut(s) 105, 405, 497, 635, 822, 897, 972
HphI GGTGA 3 cut(s) 635, 692, 986
Hpy188I TCNGA 1 cut(s) 279
Hpy188III TCNNGA 4 cut(s) 23, 383, 908, 925
Hpy99I CGWCG 1 cut(s) 454
HpyAV CCTTC 3 cut(s) 319, 389, 769
HpyCH4III ACNGT 4 cut(s) 284, 380, 613, 734
HpyCH4V TGCA 7 cut(s) 81, 192, 425, 566, 767, 829, 932
HpyF10VI GCNNNNNNNGC 2 cut(s) 873, 898
HpyF3I CTNAG 3 cut(s) 480, 510, 529
HspAI GCGC 3 cut(s) 55, 57, 876
Kzo9I GATC 1 cut(s) 154
LmnI GCTCC 3 cut(s) 48, 181, 810
Lsp1109I GCAGC 3 cut(s) 65, 176, 751
LweI GCATC 1 cut(s) 816
MaeIII GTNAC 3 cut(s) 296, 398, 589
MalI GATC 1 cut(s) 156
MboI GATC 1 cut(s) 154
MboII GAAGA 2 cut(s) 682, 752
MflI RGATCY 1 cut(s) 154
MhlI GDGCHC 3 cut(s) 39, 729, 903
MluCI AATT 5 cut(s) 119, 567, 573, 844, 933
MlyI GAGTC 2 cut(s) 140, 692
MnlI CCTC 7 cut(s) 71, 285, 385, 666, 688, 786, 866
MseI TTAA 5 cut(s) 234, 369, 639, 801, 936
MslI CAYNNNNRTG 3 cut(s) 381, 452, 720
MspI CCGG 7 cut(s) 105, 405, 497, 635, 822, 897, 972
MspR9I CCNGG 9 cut(s) 105, 405, 406, 497, 506, 822, 823, 898, 972
Mva1269I GAATGC 1 cut(s) 934
MvaI CCWGG 1 cut(s) 506
MvnI CGCG 2 cut(s) 57, 842
MwoI GCNNNNNNNGC 2 cut(s) 873, 898
NciI CCSGG 8 cut(s) 105, 405, 406, 497, 822, 823, 898, 972
NcoI CCATGG 1 cut(s) 289
NdeII GATC 1 cut(s) 154
NlaIV GGNNCC 4 cut(s) 156, 780, 797, 923
NmeAIII GCCGAG 1 cut(s) 691
NmuCI GTSAC 1 cut(s) 296
NspI RCATGY 3 cut(s) 55, 764, 896
PaeI GCATGC 2 cut(s) 55, 896
PaeR7I CTCGAG 1 cut(s) 790
PagI TCATGA 1 cut(s) 382
PauI GCGCGC 1 cut(s) 55
PceI AGGCCT 1 cut(s) 753
PctI GAATGC 1 cut(s) 934
PfeI GAWTC 4 cut(s) 19, 141, 216, 409
PfoI TCCNGGA 1 cut(s) 103
PkrI GCNGC 3 cut(s) 80, 191, 766
PleI GAGTC 2 cut(s) 140, 691
PpsI GAGTC 2 cut(s) 140, 691
PpuMI RGGWCCY 2 cut(s) 778, 796
Psp124BI GAGCTC 1 cut(s) 39
Psp5II RGGWCCY 2 cut(s) 778, 796
Psp6I CCWGG 1 cut(s) 504
PspEI GGTNACC 1 cut(s) 398
PspGI CCWGG 1 cut(s) 504
PspN4I GGNNCC 4 cut(s) 156, 780, 797, 923
PspPI GGNCC 2 cut(s) 778, 796
PspPPI RGGWCCY 2 cut(s) 778, 796
PspXI VCTCGAGB 1 cut(s) 790
PstNI CAGNNNCTG 1 cut(s) 479
PsuI RGATCY 1 cut(s) 154
PteI GCGCGC 1 cut(s) 55
RsaI GTAC 3 cut(s) 254, 308, 983
RsaNI GTAC 3 cut(s) 253, 307, 982
RseI CAYNNNNRTG 3 cut(s) 381, 452, 720
SacI GAGCTC 1 cut(s) 39
SaqAI TTAA 5 cut(s) 234, 369, 639, 801, 936
SatI GCNGC 3 cut(s) 79, 190, 765
Sau3AI GATC 1 cut(s) 154
Sau96I GGNCC 2 cut(s) 778, 796
ScaI AGTACT 1 cut(s) 254
SchI GAGTC 2 cut(s) 140, 692
ScrFI CCNGG 9 cut(s) 105, 405, 406, 497, 506, 822, 823, 898, 972
SduI GDGCHC 3 cut(s) 39, 729, 903
SfaNI GCATC 1 cut(s) 816
Sfr274I CTCGAG 1 cut(s) 790
SinI GGWCC 2 cut(s) 778, 796
SlaI CTCGAG 1 cut(s) 790
SmaI CCCGGG 2 cut(s) 406, 823
SmiMI CAYNNNNRTG 3 cut(s) 381, 452, 720
SmlI CTYRAG 1 cut(s) 790
SmoI CTYRAG 1 cut(s) 790
SphI GCATGC 2 cut(s) 55, 896
Sse9I AATT 5 cut(s) 119, 567, 573, 844, 933
SseBI AGGCCT 1 cut(s) 753
SsiI CCGC 2 cut(s) 5, 840
SstI GAGCTC 1 cut(s) 39
StuI AGGCCT 1 cut(s) 753
StyD4I CCNGG 9 cut(s) 103, 403, 404, 495, 504, 820, 821, 896, 970
StyI CCWWGG 1 cut(s) 289
TaaI ACNGT 4 cut(s) 284, 380, 613, 734
TaqI TCGA 4 cut(s) 144, 214, 729, 791
TasI AATT 5 cut(s) 119, 567, 573, 844, 933
TatI WGTACW 2 cut(s) 252, 981
TfiI GAWTC 4 cut(s) 19, 141, 216, 409
Tru1I TTAA 5 cut(s) 234, 369, 639, 801, 936
Tru9I TTAA 5 cut(s) 234, 369, 639, 801, 936
TscAI CASTG 3 cut(s) 423, 480, 667
TseFI GTSAC 1 cut(s) 296
TseI GCWGC 3 cut(s) 78, 189, 764
Tsp45I GTSAC 1 cut(s) 296
TspDTI ATGAA 1 cut(s) 618
TspGWI ACGGA 3 cut(s) 129, 139, 263
TspMI CCCGGG 2 cut(s) 404, 821
TspRI CASTG 3 cut(s) 423, 480, 667
VpaK11BI GGWCC 2 cut(s) 778, 796
XapI RAATTY 1 cut(s) 573
XceI RCATGY 3 cut(s) 55, 764, 896
XhoI CTCGAG 1 cut(s) 790
XmaI CCCGGG 2 cut(s) 404, 821
ZrmI AGTACT 1 cut(s) 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.