MD06G1232100.v1.1

NF-X1-type zinc finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0011120
Physical Location & Seq
Forward (+)
36306323 .. 36310853
4531 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1232100.v1.1.491

Sequence Viewer

Length: 3489 bp
ATGAGTTTTCGTGTCCAAAATGAGCGAAGGGATAGGTCCAGGTTTCCTGCTCAGCCTGCTACTCAGCCTACTCAGCCTGCTCGTCGAGAGTGGGTGCTGAGAGGTACCGACCCTACCACTACCACTGCTGCTGTTAACCCGCCACCAAGAGTCTACCCGAACAACCCGAATGTCAATGCCAACCAGCCTAATCCAAGATTCAATCCAAACAACCCGAACGGCAATGTTAGCCAGCCTAATCCAAGATTTAATCCAAGCAACCTGAATGGCAATCTCAGCCAGCCTAATCCAAGATTCAATCCTAACAACCTGAATGGGAATGCTGGCCTGCCTAATCATAGCTCTGTTCCACCATCAGAGATTCGGCCACACAGAGGAGGAAATAATGGCGTTATTAAAGGCCATATGGGCCAGTCAGTGAACCATAGAAGGGAGAGGGGAAGGAGTGAGAATCAGGAGGAAAAGGGATTGAAGGACTCGAATTTGCCCCAGCTTGTGCAAGAAATTCAGGATAAGCTGACAAGGGGCACTGTCGAGTGCATGATTTGTTATGAAATGGTGAGGAGGTCTGCACCTGTTTGGTCCTGCTCAAGCTGTTACTCAATTTTCCATCTGAATTGCATCAAGAAATGGGCTCGAGCTCCCACTTCTATTGACATGTCTGCGGAGAAGAATCAGGGGTTTAATTGGCGGTGTCCTGGATGTCAGTCTGTCCAGCTCACATCTTCAAAGGAGATTCGATATGTTTGCTTTTGTGGCAAGAGGACAGACCCGCCTTCAGATTTGTATTTGACACCGCATTCGTGTGGTGAGCCTTGCGGGAAGCAACTCGAGAGGGAGGTCCCAGGCAATGGTGTGAGTAAGGAGGATCTTTGCCCTCACATTTGTGTCTTGCAGTGCCACCCAGGTCCATGCCCTCCGTGTAAAGCATTTGCCCCACCACGTTTATGCCCTTGTGGAAAGAAAACAATCACAACGAGATGTTCTGACCGGGCATCTGTTCTGACTTGTGGTCAGGATTGTAATAAGCTGCTTGATTGTTGGCGTCATCGCTGCGAGCGGACTTGCCATGTGGGTCCTTGTGATCCTTGTCAGGTTCTGGTTGATGCTTCCTGCTTTTGCAAGAAAAAGGTGGAGGTTGTTCTCTGTGGAGACATGACTGTTAAGGGAGAGGTGAAAGCAGAAGATGGTGTTTTTTCATGTAGTTCCACCTGTGGAAAAATGCTTAGCTGCGGTAATCATTCCTGCAGTGAGGTTTGCCATCCAGGGCCGTGCGGAGATTGCAATTTAATGCCAACTAGGATTAAGACATGCCACTGTGGGAAGACAAGCTTGCAAGAGGAACGACGGAGTTGTTTGGATCCGATTCCAACCTGTTCTCAATTATGTAGCAAGTCCCTCCCTTGTGAGATGCACCAGTGTCAAGAGGTGTGCCATACTGGGGATTGCCCACCTTGTTTGGTTGAAGTGACCCAAAAATGTCGTTGTGGGTCAACTTCTCGGACTGCGGAATGCTTTAAAACCACAATGGAGAATGAGAAATTCACTTGTGATAAGCCTTGTGGGCGGAAGAAGAATTGTGGAAGGCACAGGTGCAGCGAGAGGTGCTGTCCTCTTTCTAATTTGAATAATGCTCTCTTGGGGAATTGGGATCCTCACTTTTGCTCTATGTCCTGTGGGAAGAAGTTAAGGTGCGGTCAGCATTCTTGCGAATCGCTTTGTCACAGCGGCCACTGCCCTCCCTGCCTTGATACAATCTTCACGGACTTGACCTGTGCATGTGGGAGGACTTCAATCCCTCCTCCACTGCCATGCGGTACCCCTCCCCCATCATGTCAGCTCCCGTGTTCAGTCCCTCAGCCTTGTGGGCATACATCCTCTCACAGCTGCCACTTTGGAGACTGTCCACCATGTGCGGTGCCTGTGGCAAAGGAGTGCATTGGTGGGCATGTGGTCCTCAGGAACATTCCTTGTGGGTCGAGGGACATCAAATGTAACAAGCTCTGTGGGAAGACAAGGCAGTGTGGTATGCATGCTTGTGGCAGGACTTGTCACCCGCCTCCCTGTGATACATCTTGTTTGGCAGAACAAGGTTCAAAAACTTCTTGTGGACAGATATGTGGTGCTCCTAGGAGAGATTGCAGGCATACATGTACTTCACTTTGTCACCCTTATGCTTCTTGTCCTGATAGCAGCTGTGATTTTCCTGTCACAATCACTTGTTCTTGTGGCCGGATGACAGCTAGTGTTCCTTGTGATTCCGGTGGAAGCAATGCTAGTTTCAAGGCCGATACTGTTTATGAAGCTTCCATTGTTCAAAGGTTGCCAGCACCACTGCAACCAATTGAATCAACCAGCAAGAATATCCCGCTCGGACAGAGGAAGCTTATGTGTGATGATGAATGTGCTAAGATGGAGCGGAAACGGGTTCTTGCAGATGCTTTTGATATAACTCCTCCAAACTTGGATGCGCTCCATTTTGGTGAGAGTTCTGCTGTTTCTGAGTTGCTTTCAGACCTTCTTAGACGCGATCCCAAGTGGGTATTATCTGTGGAGGAGAGATGCAAGTACTTGGTGCTCGGCAAGAGTAGAGGTGCTACAAGTGGTCTCAAGGTTCATGTTTTCTGCCCGATGCTGAAGGAGAAGAGGGATGTAGTTCGGATGATCGCTGAAAGATGGAAGCTTGCAGTTCAAGCCGCTGGCTGGGAGCCCAAGCGATTTATTGTGGTTCATGTTACACCTAAATCCAAAGCCCCCGCTCGGATACTTGGGGTTAAGGGCACAACAACAGTGAGTGCACCCAAGCCTCCGTCTTATGATCATTTAGTGGACATGGATCCCAGGCTTGTAGTTTCATTCCCAGATCTCCCTAGAGATGCAGATATAAGTGCACTTGTATTGAGGTTTGGTGGGGAGTGTGAGTTAGTCTGGTTGAACGATAAGAATGCACTAGCCGTATTCAACGACCCTGCTCGAGCAGCAACTGCAATGAGGAGGTTGGATAATGGTGCACTATATCATGGCGCTATTGTTGTTCACTCCAATGGCAGTGCATCTATGGCGGCATCAGGTTCTAATGCTTGGGGTGGATTGGGGACATCTAGGGAAGGAGGAGCTTCTGCAGTGCTGATGGGTAATCCGTGGAAGAAGACTGTTACACGAGAGTCCGGTTGGAGGGAAGATTCATGGGGCGAGGAAGAGTGGCCTGGCAGTTCTACAGATGCGCCGGCAAACGTGTGGAATAAAAAAGCACCAATTGCTGCCTCAGTAAATCGATGGAGTGTACTGGACGGTGACACGGCATTGGGCTCATCCGCTTCATCTCTCAGAGTTGAGGACTATAGAAAACTGTCTTTGGGTCCATTGAATTCGGCCTTGGACTCGAAAGCGAGTGGTTCGAGTTCGAGTTCCACTTTTGAAGGGCAGCCTGTTGGAGTTATTGCAGAAACACCTGAAGTGGTTGATGACTGGGAGAAGGCTTATGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000122 GO:0000976 GO:0000977 GO:0000981 GO:0000982 GO:0001012 GO:0001067 GO:0001078 GO:0001227 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006082 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006970 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009416 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009636 GO:0009642 GO:0009651 GO:0009696 GO:0009697 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010188 GO:0010310 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0010817 GO:0016053 GO:0016070 GO:0016999 GO:0017000 GO:0017144 GO:0018130 GO:0018958 GO:0019219 GO:0019222 GO:0019438 GO:0019752 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032774 GO:0032787 GO:0034641 GO:0034645 GO:0034654 GO:0042221 GO:0042445 GO:0042446 GO:0042537 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0046189 GO:0046394 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051193 GO:0051252 GO:0051253 GO:0051704 GO:0051707 GO:0060255 GO:0065007 GO:0065008 GO:0071704 GO:0072330 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:0098542 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901615 GO:1901617 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:2000112 GO:2000113 GO:2000377 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko05165 map05165
Pfam Domains
Protein Families
K_trans R3H zf-NF-X1

Protein Analysis

1163

Amino Acids

126.42

Weight (kDa)

8.48

Isoelectric Point (pI)

50.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_NFXL1 PF24435 936 - 1013 9.3e-42 NFXL1 RRM-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011120)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10170 AT1G10170 AT1G10170 AT1G10170
fragaria_vesca FvH4_5g17660
malus_domestica MD06G1232100.v1.1 MD14G1238900.v1.1
prunus_persica Prupe.5G236400_v2.0.a1
pyrus_communis pycom06g20620 pycom14g19990
rosa_chinensis RchiOBHm_Chr7g0178011
rosa_laevigata RLG00000005482
rosa_multiflora Rmu_sc0013399.1_g000001
rosa_roxburghii Rroxscaffold_3G00275290
rosa_rugosa Rorug06G0412300
rosa_samantha Rh7AG011800 Rh7BG011200 Rh7CG011800 Rh7DG011800
rosa_wichuraiana Rw7G000930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 104, 1817
AccB1I GGYRCC 3 cut(s) 104, 1817, 1918
AccB7I CCANNNNNTGG 1 cut(s) 851
AccBSI CCGCTC 4 cut(s) 1060, 2371, 2419, 2759
AccI GTMKAC 1 cut(s) 153
AccII CGCG 1 cut(s) 2529
AclWI GGATC 9 cut(s) 876, 1079, 1355, 1368, 1646, 1659, 2525, 2831, 2844
AcoI YGGCCR 3 cut(s) 365, 1729, 2230
AcsI RAATTY 4 cut(s) 481, 504, 1541, 3367
AcuI CTGAAG 3 cut(s) 762, 2657, 3474
AcyI GRCGYC 1 cut(s) 1045
AdeI CACNNNGTG 1 cut(s) 1913
AfaI GTAC 5 cut(s) 106, 1819, 2155, 2570, 3285
AfiI CCNNNNNNNGG 7 cut(s) 374, 430, 851, 1441, 2703, 2760, 3174
AflIII ACRYGT 3 cut(s) 657, 2150, 3234
AhdI GACNNNNNGTC 1 cut(s) 1011
AjnI CCWGG 7 cut(s) 38, 697, 844, 904, 1264, 2840, 3205
AjuI GAANNNNNNNTTGG 2 cut(s) 3359, 3391
AleI CACNNNNGTG 1 cut(s) 885
Alw21I GWGCWC 6 cut(s) 643, 2128, 2580, 2800, 2893, 3013
Alw26I GTCTC 3 cut(s) 1146, 1893, 2612
Alw44I GTGCAC 3 cut(s) 2796, 2889, 3009
AlwI GGATC 9 cut(s) 876, 1079, 1355, 1368, 1646, 1659, 2525, 2831, 2844
AlwNI CAGNNNCTG 2 cut(s) 1099, 2984
Ama87I CYCGRG 3 cut(s) 636, 830, 2973
ApaLI GTGCAC 3 cut(s) 2796, 2889, 3009
ApoI RAATTY 4 cut(s) 481, 504, 1541, 3367
ArsI GACNNNNNNTTYG 2 cut(s) 784, 816
Asp718I GGTACC 2 cut(s) 104, 1817
AspA2I CCTAGG 1 cut(s) 2129
AspLEI GCGC 3 cut(s) 2473, 3026, 3226
AspS9I GGNCC 9 cut(s) 36, 409, 582, 841, 908, 1076, 1268, 1954, 3359
AsuC2I CCSGG 1 cut(s) 992
AsuHPI GGTGA 7 cut(s) 571, 821, 1186, 2045, 2159, 2495, 3305
AvaI CYCGRG 3 cut(s) 636, 830, 2973
AvaII GGWCC 7 cut(s) 36, 582, 841, 908, 1076, 1954, 3359
AvrII CCTAGG 1 cut(s) 2129
AxyI CCTNAGG 1 cut(s) 1958
BaeGI GKGCMC 5 cut(s) 530, 2783, 2800, 2893, 3013
BamHI GGATCC 3 cut(s) 1360, 1651, 2836
BanI GGYRCC 3 cut(s) 104, 1817, 1918
BanII GRGCYC 4 cut(s) 637, 643, 2712, 3311
BauI CACGAG 1 cut(s) 3159
BbsI GAAGAC 3 cut(s) 1331, 2018, 3155
Bbv12I GWGCWC 6 cut(s) 643, 2128, 2580, 2800, 2893, 3013
BbvCI CCTCAGC 1 cut(s) 1857
BccI CCATC 9 cut(s) 361, 618, 1181, 1269, 1837, 2407, 2670, 3124, 3270
BceAI ACGGC 4 cut(s) 235, 1255, 2939, 3315
BcgI CGANNNNNNTGC 4 cut(s) 729, 763, 2927, 2961
BciT130I CCWGG 7 cut(s) 40, 699, 846, 906, 1266, 2842, 3207
BciVI GTATCC 1 cut(s) 2757
BclI TGATCA 1 cut(s) 2818
BcnI CCSGG 1 cut(s) 992
BcoDI GTCTC 3 cut(s) 1146, 1893, 2612
BfaI CTAG 7 cut(s) 1299, 2130, 2244, 2277, 2871, 2951, 3102
BfmI CTRYAG 4 cut(s) 1246, 3120, 3216, 3340
BfoI RGCGCY 1 cut(s) 3027
BfuI GTATCC 1 cut(s) 2757
BglI GCCNNNNNGGC 3 cut(s) 408, 1564, 1867
BglII AGATCT 1 cut(s) 2863
BlnI CCTAGG 1 cut(s) 2129
BlpI GCTNAGC 2 cut(s) 51, 1226
BmcAI AGTACT 1 cut(s) 2570
Bme1390I CCNGG 8 cut(s) 40, 699, 846, 906, 992, 1266, 2842, 3207
Bme18I GGWCC 7 cut(s) 36, 582, 841, 908, 1076, 1954, 3359
BmeRI GACNNNNNGTC 1 cut(s) 1011
BmeT110I CYCGRG 3 cut(s) 636, 830, 2973
BmgT120I GGNCC 9 cut(s) 36, 409, 582, 841, 908, 1076, 1268, 1954, 3359
BmrFI CCNGG 8 cut(s) 40, 699, 846, 906, 992, 1266, 2842, 3207
BmrI ACTGGG 2 cut(s) 1449, 3478
BmuI ACTGGG 2 cut(s) 1449, 3478
BpiI GAAGAC 3 cut(s) 1331, 2018, 3155
Bpu10I CCTNAGC 1 cut(s) 1857
Bpu1102I GCTNAGC 2 cut(s) 51, 1226
BpuEI CTTGAG 2 cut(s) 574, 2594
BpuMI CCSGG 1 cut(s) 992
Bsa29I ATCGAT 1 cut(s) 3274
BsaHI GRCGYC 1 cut(s) 1045
BsaI GGTCTC 1 cut(s) 2612
BsaJI CCNNGG 7 cut(s) 844, 904, 1265, 2129, 2840, 3140, 3375
BsaWI WCCGGW 2 cut(s) 2261, 3167
BsaXI ACNNNNNCTCC 2 cut(s) 2906, 2936
Bsc4I CCNNNNNNNGG 7 cut(s) 374, 430, 851, 1441, 2703, 2760, 3174
Bse118I RCCGGY 1 cut(s) 3226
Bse1I ACTGG 5 cut(s) 412, 1417, 1444, 3291, 3473
Bse21I CCTNAGG 1 cut(s) 1958
Bse3DI GCAATG 4 cut(s) 229, 856, 2278, 2994
BseBI CCWGG 7 cut(s) 40, 699, 846, 906, 1266, 2842, 3207
BseCI ATCGAT 1 cut(s) 3274
BseDI CCNNGG 7 cut(s) 844, 904, 1265, 2129, 2840, 3140, 3375
BseGI GGATG 8 cut(s) 707, 1261, 1874, 2241, 2473, 2656, 2667, 3311
BseLI CCNNNNNNNGG 7 cut(s) 374, 430, 851, 1441, 2703, 2760, 3174
BseMI GCAATG 4 cut(s) 229, 856, 2278, 2994
BseNI ACTGG 5 cut(s) 412, 1417, 1444, 3291, 3473
BseRI GAGGAG 7 cut(s) 390, 577, 1791, 2445, 2570, 3007, 3126
BseSI GKGCMC 5 cut(s) 530, 2783, 2800, 2893, 3013
BseYI CCCAGC 2 cut(s) 489, 2703
BsgI GTGCAG 2 cut(s) 555, 1615
Bsh1236I CGCG 1 cut(s) 2529
BshNI GGYRCC 3 cut(s) 104, 1817, 1918
BshVI ATCGAT 1 cut(s) 3274
BsiHKAI GWGCWC 6 cut(s) 643, 2128, 2580, 2800, 2893, 3013
BsiHKCI CYCGRG 3 cut(s) 636, 830, 2973
BsiSI CCGG 5 cut(s) 991, 2233, 2262, 3168, 3227
BslFI GGGAC 5 cut(s) 827, 1381, 1838, 1997, 3109
BslI CCNNNNNNNGG 7 cut(s) 374, 430, 851, 1441, 2703, 2760, 3174
BsmAI GTCTC 3 cut(s) 1146, 1893, 2612
BsmFI GGGAC 5 cut(s) 827, 1381, 1838, 1997, 3109
BsmI GAATGC 5 cut(s) 325, 799, 1517, 1702, 2950
Bso31I GGTCTC 1 cut(s) 2612
BsoBI CYCGRG 3 cut(s) 636, 830, 2973
Bsp143I GATC 9 cut(s) 868, 1084, 1360, 1651, 2530, 2664, 2818, 2836, 2863
Bsp1720I GCTNAGC 2 cut(s) 51, 1226
BspDI ATCGAT 1 cut(s) 3274
BspFNI CGCG 1 cut(s) 2529
BspMAI CTGCAG 2 cut(s) 1250, 3124
BspPI GGATC 9 cut(s) 876, 1079, 1355, 1368, 1646, 1659, 2525, 2831, 2844
BspT107I GGYRCC 3 cut(s) 104, 1817, 1918
BspTNI GGTCTC 1 cut(s) 2612
BsrBI CCGCTC 4 cut(s) 1060, 2371, 2419, 2759
BsrDI GCAATG 4 cut(s) 229, 856, 2278, 2994
BsrFI RCCGGY 1 cut(s) 3226
BsrI ACTGG 5 cut(s) 412, 1417, 1444, 3291, 3473
BssAI RCCGGY 1 cut(s) 3226
BssECI CCNNGG 7 cut(s) 844, 904, 1265, 2129, 2840, 3140, 3375
BssMI GATC 9 cut(s) 868, 1084, 1360, 1651, 2530, 2664, 2818, 2836, 2863
BssNI GRCGYC 1 cut(s) 1045
BssSI CACGAG 1 cut(s) 3159
BssT1I CCWWGG 2 cut(s) 2129, 3375
Bst2BI CACGAG 1 cut(s) 3159
Bst2UI CCWGG 7 cut(s) 40, 699, 846, 906, 1266, 2842, 3207
Bst4CI ACNGT 9 cut(s) 532, 1162, 1319, 1904, 2296, 2791, 3154, 3293, 3351
Bst6I CTCTTC 2 cut(s) 2639, 3192
BstACI GRCGYC 1 cut(s) 1045
BstAPI GCANNNNNTGC 2 cut(s) 2984, 3257
BstDSI CCRYGG 1 cut(s) 3140
BstF5I GGATG 8 cut(s) 707, 1261, 1874, 2241, 2473, 2656, 2667, 3311
BstFNI CGCG 1 cut(s) 2529
BstH2I RGCGCY 1 cut(s) 3027
BstHHI GCGC 3 cut(s) 2473, 3026, 3226
BstKTI GATC 9 cut(s) 871, 1087, 1363, 1654, 2533, 2667, 2821, 2839, 2866
BstMAI GTCTC 3 cut(s) 1146, 1893, 2612
BstMBI GATC 9 cut(s) 868, 1084, 1360, 1651, 2530, 2664, 2818, 2836, 2863
BstNI CCWGG 7 cut(s) 40, 699, 846, 906, 1266, 2842, 3207
BstNSI RCATGY 6 cut(s) 661, 1314, 1782, 1952, 2036, 2154
BstSCI CCNGG 8 cut(s) 38, 697, 844, 904, 990, 1264, 2840, 3205
BstSFI CTRYAG 4 cut(s) 1246, 3120, 3216, 3340
BstSLI GKGCMC 5 cut(s) 530, 2783, 2800, 2893, 3013
BstUI CGCG 1 cut(s) 2529
BstV2I GAAGAC 3 cut(s) 1331, 2018, 3155
BstX2I RGATCY 5 cut(s) 868, 1360, 1651, 2836, 2863
BstYI RGATCY 5 cut(s) 868, 1360, 1651, 2836, 2863
Bsu15I ATCGAT 1 cut(s) 3274
Bsu36I CCTNAGG 1 cut(s) 1958
BsuI GTATCC 1 cut(s) 2757
BsuTUI ATCGAT 1 cut(s) 3274
BtgI CCRYGG 1 cut(s) 3140
BtgZI GCGATG 1 cut(s) 1034
BtsCI GGATG 8 cut(s) 707, 1261, 1874, 2241, 2473, 2656, 2667, 3311
BtsI GCAGTG 9 cut(s) 123, 902, 1255, 1732, 1805, 2027, 2333, 3055, 3129
CaiI CAGNNNCTG 2 cut(s) 1099, 2984
CfoI GCGC 3 cut(s) 2473, 3026, 3226
Cfr10I RCCGGY 1 cut(s) 3226
Cfr13I GGNCC 9 cut(s) 36, 409, 582, 841, 908, 1076, 1268, 1954, 3359
ClaI ATCGAT 1 cut(s) 3274
CseI GACGC 2 cut(s) 1034, 2535
Csp6I GTAC 5 cut(s) 105, 1818, 2154, 2569, 3284
CspCI CAANNNNNGTGG 6 cut(s) 634, 669, 1440, 1475, 1987, 2022
CviQI GTAC 5 cut(s) 105, 1818, 2154, 2569, 3284
DpnI GATC 9 cut(s) 870, 1086, 1362, 1653, 2532, 2666, 2820, 2838, 2865
DpnII GATC 9 cut(s) 868, 1084, 1360, 1651, 2530, 2664, 2818, 2836, 2863
DraI TTTAAA 1 cut(s) 1519
DraIII CACNNNGTG 1 cut(s) 1913
DriI GACNNNNNGTC 1 cut(s) 1011
EaeI YGGCCR 3 cut(s) 365, 1729, 2230
Eam1104I CTCTTC 2 cut(s) 2639, 3192
Eam1105I GACNNNNNGTC 1 cut(s) 1011
EarI CTCTTC 2 cut(s) 2639, 3192
EciI GGCGGA 1 cut(s) 1582
Ecl136II GAGCTC 1 cut(s) 641
Eco130I CCWWGG 2 cut(s) 2129, 3375
Eco24I GRGCYC 4 cut(s) 637, 643, 2712, 3311
Eco31I GGTCTC 1 cut(s) 2612
Eco47I GGWCC 7 cut(s) 36, 582, 841, 908, 1076, 1954, 3359
Eco53kI GAGCTC 1 cut(s) 641
Eco57I CTGAAG 3 cut(s) 762, 2657, 3474
Eco81I CCTNAGG 1 cut(s) 1958
Eco88I CYCGRG 3 cut(s) 636, 830, 2973
EcoICRI GAGCTC 1 cut(s) 641
EcoO109I RGGNCCY 2 cut(s) 841, 1076
EcoRI GAATTC 1 cut(s) 3367
EcoRII CCWGG 7 cut(s) 38, 697, 844, 904, 1264, 2840, 3205
EcoT14I CCWWGG 2 cut(s) 2129, 3375
EcoT22I ATGCAT 1 cut(s) 2034
EcoT38I GRGCYC 4 cut(s) 637, 643, 2712, 3311
ErhI CCWWGG 2 cut(s) 2129, 3375
FalI AAGNNNNNCTT 2 cut(s) 1316, 1348
FaqI GGGAC 5 cut(s) 827, 1381, 1838, 1997, 3109
FauI CCCGC 6 cut(s) 147, 780, 812, 2064, 2376, 2764
FauNDI CATATG 1 cut(s) 405
FbaI TGATCA 1 cut(s) 2818
FblI GTMKAC 1 cut(s) 153
FokI GGATG 8 cut(s) 714, 1248, 1861, 2248, 2480, 2663, 2674, 3298
FriOI GRGCYC 4 cut(s) 637, 643, 2712, 3311
FspBI CTAG 7 cut(s) 1299, 2130, 2244, 2277, 2871, 2951, 3102
GlaI GCGC 3 cut(s) 2472, 3025, 3225
GsaI CCCAGC 2 cut(s) 493, 2707
HaeII RGCGCY 1 cut(s) 3027
HapII CCGG 5 cut(s) 991, 2233, 2262, 3168, 3227
HgaI GACGC 2 cut(s) 1034, 2535
HhaI GCGC 3 cut(s) 2473, 3026, 3226
Hin1I GRCGYC 1 cut(s) 1045
Hin6I GCGC 3 cut(s) 2471, 3024, 3224
HinP1I GCGC 3 cut(s) 2471, 3024, 3224
HincII GTYRAC 2 cut(s) 136, 1494
HindII GTYRAC 2 cut(s) 136, 1494
HindIII AAGCTT 4 cut(s) 1330, 2304, 2384, 2681
HpaI GTTAAC 1 cut(s) 136
HpaII CCGG 5 cut(s) 991, 2233, 2262, 3168, 3227
HphI GGTGA 7 cut(s) 571, 821, 1186, 2045, 2159, 2495, 3305
Hpy188III TCNNGA 9 cut(s) 86, 455, 509, 625, 832, 1016, 1424, 1960, 2186
Hpy99I CGWCG 2 cut(s) 87, 1350
HpyCH4III ACNGT 9 cut(s) 532, 1162, 1319, 1904, 2296, 2791, 3154, 3293, 3351
HpyCH4IV ACGT 2 cut(s) 943, 3234
HpySE526I ACGT 2 cut(s) 943, 3234
Hsp92I GRCGYC 1 cut(s) 1045
HspAI GCGC 3 cut(s) 2471, 3024, 3224
KpnI GGTACC 2 cut(s) 108, 1821
KroI GCCGGC 1 cut(s) 3226
KroNI GCCGGC 1 cut(s) 3228
Ksp22I TGATCA 1 cut(s) 2818
KspAI GTTAAC 1 cut(s) 136
Kzo9I GATC 9 cut(s) 868, 1084, 1360, 1651, 2530, 2664, 2818, 2836, 2863
LmnI GCTCC 7 cut(s) 646, 1845, 2131, 2416, 2478, 2707, 3113
MaeI CTAG 7 cut(s) 1299, 2130, 2244, 2277, 2871, 2951, 3102
MaeII ACGT 2 cut(s) 943, 3234
MalI GATC 9 cut(s) 870, 1086, 1362, 1653, 2532, 2666, 2820, 2838, 2865
MbiI CCGCTC 4 cut(s) 1060, 2371, 2419, 2759
MboI GATC 9 cut(s) 868, 1084, 1360, 1651, 2530, 2664, 2818, 2836, 2863
MfeI CAATTG 2 cut(s) 2343, 3255
MflI RGATCY 5 cut(s) 868, 1360, 1651, 2836, 2863
MlyI GAGTC 4 cut(s) 159, 470, 3173, 3374
MmeI TCCRAC 4 cut(s) 1394, 2979, 3152, 3412
Mph1103I ATGCAT 1 cut(s) 2034
MroNI GCCGGC 1 cut(s) 3226
MslI CAYNNNNRTG 8 cut(s) 804, 885, 946, 1810, 2037, 2172, 2481, 3042
MspA1I CMGCKG 4 cut(s) 1728, 1887, 2196, 2699
MspI CCGG 5 cut(s) 991, 2233, 2262, 3168, 3227
MspR9I CCNGG 8 cut(s) 40, 699, 846, 906, 992, 1266, 2842, 3207
MunI CAATTG 2 cut(s) 2343, 3255
Mva1269I GAATGC 5 cut(s) 325, 799, 1517, 1702, 2950
MvaI CCWGG 7 cut(s) 40, 699, 846, 906, 1266, 2842, 3207
MvnI CGCG 1 cut(s) 2529
NaeI GCCGGC 1 cut(s) 3228
NciI CCSGG 1 cut(s) 992
NdeI CATATG 1 cut(s) 405
NdeII GATC 9 cut(s) 868, 1084, 1360, 1651, 2530, 2664, 2818, 2836, 2863
NgoMIV GCCGGC 1 cut(s) 3226
NmeAIII GCCGAG 1 cut(s) 2559
NmuCI GTSAC 6 cut(s) 1468, 1721, 2051, 2165, 2209, 3293
NsiI ATGCAT 1 cut(s) 2034
NspI RCATGY 6 cut(s) 661, 1314, 1782, 1952, 2036, 2154
OliI CACNNNNGTG 1 cut(s) 885
PaeI GCATGC 1 cut(s) 2036
PaeR7I CTCGAG 3 cut(s) 636, 830, 2973
PciI ACATGT 2 cut(s) 657, 2150
PctI GAATGC 5 cut(s) 325, 799, 1517, 1702, 2950
PdiI GCCGGC 1 cut(s) 3228
PflMI CCANNNNNTGG 1 cut(s) 851
PfoI TCCNGGA 1 cut(s) 697
PleI GAGTC 4 cut(s) 158, 470, 3172, 3374
PpsI GAGTC 4 cut(s) 158, 470, 3172, 3374
PpuMI RGGWCCY 2 cut(s) 841, 1076
PscI ACATGT 2 cut(s) 657, 2150
Psp124BI GAGCTC 1 cut(s) 643
Psp5II RGGWCCY 2 cut(s) 841, 1076
Psp6I CCWGG 7 cut(s) 38, 697, 844, 904, 1264, 2840, 3205
PspFI CCCAGC 2 cut(s) 489, 2703
PspGI CCWGG 7 cut(s) 38, 697, 844, 904, 1264, 2840, 3205
PspPI GGNCC 9 cut(s) 36, 409, 582, 841, 908, 1076, 1268, 1954, 3359
PspPPI RGGWCCY 2 cut(s) 841, 1076
PspXI VCTCGAGB 2 cut(s) 636, 2973
PstI CTGCAG 2 cut(s) 1250, 3124
PstNI CAGNNNCTG 2 cut(s) 1099, 2984
PsuI RGATCY 5 cut(s) 868, 1360, 1651, 2836, 2863
PvuII CAGCTG 2 cut(s) 1887, 2196
RsaI GTAC 5 cut(s) 106, 1819, 2155, 2570, 3285
RsaNI GTAC 5 cut(s) 105, 1818, 2154, 2569, 3284
RseI CAYNNNNRTG 8 cut(s) 804, 885, 946, 1810, 2037, 2172, 2481, 3042
SacI GAGCTC 1 cut(s) 643
Sau3AI GATC 9 cut(s) 868, 1084, 1360, 1651, 2530, 2664, 2818, 2836, 2863
Sau96I GGNCC 9 cut(s) 36, 409, 582, 841, 908, 1076, 1268, 1954, 3359
ScaI AGTACT 1 cut(s) 2570
SchI GAGTC 4 cut(s) 159, 470, 3173, 3374
ScrFI CCNGG 8 cut(s) 40, 699, 846, 906, 992, 1266, 2842, 3207
SfcI CTRYAG 4 cut(s) 1246, 3120, 3216, 3340
SfiI GGCCNNNNNGGCC 1 cut(s) 408
Sfr274I CTCGAG 3 cut(s) 636, 830, 2973
SinI GGWCC 7 cut(s) 36, 582, 841, 908, 1076, 1954, 3359
SlaI CTCGAG 3 cut(s) 636, 830, 2973
SmiMI CAYNNNNRTG 8 cut(s) 804, 885, 946, 1810, 2037, 2172, 2481, 3042
SmlI CTYRAG 5 cut(s) 589, 636, 830, 2609, 2973
SmoI CTYRAG 5 cut(s) 589, 636, 830, 2609, 2973
SphI GCATGC 1 cut(s) 2036
SspMI CTAG 7 cut(s) 1299, 2130, 2244, 2277, 2871, 2951, 3102
SstI GAGCTC 1 cut(s) 643
StyD4I CCNGG 8 cut(s) 38, 697, 844, 904, 990, 1264, 2840, 3205
StyI CCWWGG 2 cut(s) 2129, 3375
TaaI ACNGT 9 cut(s) 532, 1162, 1319, 1904, 2296, 2791, 3154, 3293, 3351
TaiI ACGT 2 cut(s) 946, 3237
TatI WGTACW 3 cut(s) 2153, 2568, 3283
TauI GCSGC 3 cut(s) 1731, 2699, 3065
TseFI GTSAC 6 cut(s) 1468, 1721, 2051, 2165, 2209, 3293
Tsp45I GTSAC 6 cut(s) 1468, 1721, 2051, 2165, 2209, 3293
TspDTI ATGAA 9 cut(s) 567, 1188, 2316, 2415, 2606, 2720, 2844, 3174, 3309
TspGWI ACGGA 5 cut(s) 909, 1363, 1778, 2799, 3129
Van91I CCANNNNNTGG 1 cut(s) 851
VneI GTGCAC 3 cut(s) 2796, 2889, 3009
VpaK11BI GGWCC 7 cut(s) 36, 582, 841, 908, 1076, 1954, 3359
XapI RAATTY 4 cut(s) 481, 504, 1541, 3367
XceI RCATGY 6 cut(s) 661, 1314, 1782, 1952, 2036, 2154
XhoI CTCGAG 3 cut(s) 636, 830, 2973
XmaJI CCTAGG 1 cut(s) 2129
XmiI GTMKAC 1 cut(s) 153
XspI CTAG 7 cut(s) 1299, 2130, 2244, 2277, 2871, 2951, 3102
ZrmI AGTACT 1 cut(s) 2570
Zsp2I ATGCAT 1 cut(s) 2034
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.