MD14G1238900.v1.1

NF-X1-type zinc finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0011120
Physical Location & Seq
Forward (+)
31806410 .. 31810952
4543 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1238900.v1.1.491

Sequence Viewer

Length: 3417 bp
ATGAGTTCTCATGTCCGAAATGATCGAAGGGATAGGTCCAGGTTTCCTGCTCAGCCTACTCAGCCTGCTCGTCGAGAGTGGGTGCCGAGAGGATCCAACCCTGCCATTGCCACTGCTGCCGTGAACCCGCCTCCACAAGTCAATCCAAACAACCAGAATGGCAATGTCACCCAGCCTAATCTAAGATTCAATCCAAACAACCTGAATGGCAATGTCAGCCAGCCTACTCCAAGATTCAACCTGGACAACCCGAATAGCAATGTCAGCCAGCCTAATCATAGCTCTGTTCCATCAGAGATTCGGCCGCATAGAGGAGGAAATAATGGCGTTAGAGGACAGGGTCGGCCGGTGAACCATAGAAGGGAGAGGGGAAGGAATGACAATCAAGAGGAGAAGGGATTGAAGGACTCAAATTTGCCCCAGCTTGTGCAAGAAATTCAGGATAAGCTGACGAAGGGCACTGTCGAGTGTATGATTTGTTATGATATGGTGAGGAGGTCTGCACCTGTTTGGTCCTGCTCAAGTTGCTACTCAATTTTCCATCTGACTTGCATCAAGAAATGGGCTCGAGCTCCCACTTCTATCGACATGTCTGCGGGGAAGAATCAGGGGTTTAATTGGCGGTGTCCTGGATGTCAATCTGTACAGCTCACTTCGTCAAAGGAGATTCGGTATGATTGCTTTTGTGGCAAGAGGACAGACCCACCTTCAGATTTGTATTTGACACCACATTCATGTGGCGAGCCTTGCGGGAAGCAACTCGAGAGGGAGGTACCGGGTAAGGGTGTGAGTAAGGACGATCTTTGCCCTCACGTTTGTGTCTTGCAGTGCCACCCAGGTCCATGCCCTCCTTGTAAAGCATTTGCCCCTCCACGTTTATGCCCATGTGGAAAGAAAATAATCACAACAAGATGTTCCGACCGGACATCTGTTCTGACTTGTGGTCAGCATTGTAATAAGCTTCTTGATTGCTGGCGTCACCGCTGTGAGCGGACTTGCCATGTGGGTCCTTGTGATCCTTGTCAGGTTCTTGTTGATGCGTCCTGCTTTTGCAAGAAAAAGGTGGAGGTTGTTCTTTGTGGAGACATGACTGTGAAGGGAGAGGTGAAGGCAGAAGATGGTGTTTTTTCATGCAGTTCCACGTGTGGAAAAATGCTTAGCTGCTGTAATCATTCCTGCAGCGAAGTTTGCCATCCAGGGCCATGTGGAGATTGCAATTTAATGCCAAGTAAGACTAAGACATGCCACTGTGGGAAAACAAGCTTGCAAGAGGAACGACAGAGTTGTTTGGATCCGATTCCAACCTGTTCACAATTATGTGGTAAGTCTCTCCCTTGTGGGATGCACCAGTGTCAAGAGGTGTGCCATACTGGGGATTGCCCACCTTGTTTGGTTGAAGTGACCCAAAAATGTCGTTGTGGGTCAACTTCTCGGGCAGTGGAATGCTTTAAAACCACAATGGAGAATGAGAAATTTACTTGTGACAAGCCTTGTGGGCAGAAGAAGAATTGTGGAAGACATCGGTGCAGTGAGAGGTGCTGTCCCCTTTCTAATTCAAATAATGCTCTCTCAGGGGATTGGGATCCTCACTTTTGCTCTATGCCCTGTGGGAAGAAGTTAAGGTGCGGTCAGCATTCTTGTGAATCGCTTTGTCACAGTGGCCACTGCCCTCCCTGCCTTGATACAATCTTCACGGACCTGACCTGTGCCTGTGGGAGGACTTCAATCCCTCCTCCACTACCATGCGGTACCTCTCCCCCGTCATGTCAGCTGCCGTGTTCACTCCCTCAGCCTTGTGGGCATACATCTTCTCACAGCTGCCACTTTGGAGACTGTCCACCGTGTTCGGTGCCTGTGGCAAAGGAGTGCATTGGTGGGCATGTGGTCCTCAGGAACATTCCTTGTGGGTCGAGGGACATCAAGTGTAACAAGCTCTGTGGGAAGACAAGGCAGTGTGGTATGCATGCTTGTGGCAGGACTTGTCACCCACCTCCCTGTGATACATCTTGCTCAGCAGAACAAGGTTCAAAAACTTCTTGTGGGCAGATATGTGGTGCTCCTAGGAGAGATTGCAGGCATACATGTACTTCACTTTGTCACCCTTATGCTTCTTGTCCTGATAGCAGATGTGATTATTCTGTCACAATCACTTGTTCTTGTGGCCGGATGACAGCAACTGTTCCTTGTGATTCTGGTGGCAACAATGCTAGTTTCAAGGCCGATACTGTGTACGAAGCTTCTGTTATTCAAAGGTTGCCAGCACCACTTCAACCAATTGAATCAACCGGCAAGAAGATCCCGCTTGGACAGAGGAAGCTTATGTGTGATGATGAATGTGCTAAGATGGAGCGGAAACGGGTTCTTGCAGATGCTTTTGATATAGCTCCTCCAAACTTGGATGCACTCCATTTTGGTGAGAGTTCTGCTGTTTCTGAGTTGCTTTCGGACCTTCTTAGACGTGATCCCAAGTGGGTATTATCAGTGGAGGAGAGATGCAAGTACTTAGTGCTCGGCAAGGGCAGAGGTGCTACAAGTGGTCTCAAGGTTCATGTTTTCTGTCCAATGCTAAAGGAGAAGAGGGATGTAGTTCGGATGATCGCTGAAAGATGGAAGCTTGCAGTTCAAGCCGCTGGTTGGGAGCCCAAGCGATTTATTGTGGTTCATGTTACACCAAAATCAAAAGCCCCCGCTCGGATACTTGGGGTTAAGGGTACAACAACAGTTGGTGCACCCCAGCCTCCAGCTTATGATTATTTAGTAGACATGGATCCCAGGCTTGTAGTTTCATTCCCAGATCTTCCTAGAGATGCAGATATAAGTGCACTTGTATTAAGGTTTGGTGGGGAGTGTGAGTTAGTATGGTTGAACGATAAGAATGCATTAGCTGTATTCAACGATCCTGCTCGAGCAGCAACTGCAATGAGGAGGTTGGATAATGGCGCACTATATCATGGCGCTGTAGTTGTCCACTCAAATGGGAGTGCATCTATGGCGGCATCGGGTTCTAACGCTTGGGGTGGACTGGGGACAGCTAAGGAAGGTGGAGCGTCTGCAGTGCTGAGGGGTAATCCGTGGAAGAAGGCTGTTACGCGAGAGTCTGGTTGGAGGGAAGATTCATGGGGTGAGGAAGAGTGGTCAGGCAGTTCTACAGATGCGCAGGCAAACGTGTGGAATAAAGAAGTACCAATTGCTGCCTCAGTAAATCGATGGAGTGTACTAGACAGTGACACGGCATTGGGCTCATCCGCTTCATCTCCCAGGGTTGAGGACTCTAGAAAACAGCCTTTGGGTCCATCAAATTCGGGTTTGGAGTCGAAAGCAAGCGGTTCGAGTTCGAGTTCCACTTTGGCTGGGCAGCCAGTTGGAGTTATTGCAGATACACCTGAAGTTGATGACTGGGAGAAGGCTTATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000122 GO:0000976 GO:0000977 GO:0000981 GO:0000982 GO:0001012 GO:0001067 GO:0001078 GO:0001227 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006082 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006970 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009416 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009636 GO:0009642 GO:0009651 GO:0009696 GO:0009697 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010188 GO:0010310 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0010817 GO:0016053 GO:0016070 GO:0016999 GO:0017000 GO:0017144 GO:0018130 GO:0018958 GO:0019219 GO:0019222 GO:0019438 GO:0019752 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032774 GO:0032787 GO:0034641 GO:0034645 GO:0034654 GO:0042221 GO:0042445 GO:0042446 GO:0042537 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0046189 GO:0046394 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051193 GO:0051252 GO:0051253 GO:0051704 GO:0051707 GO:0060255 GO:0065007 GO:0065008 GO:0071704 GO:0072330 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:0098542 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901615 GO:1901617 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:2000112 GO:2000113 GO:2000377 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko05165 map05165
Pfam Domains
Protein Families
K_trans R3H zf-NF-X1

Protein Analysis

1139

Amino Acids

123.37

Weight (kDa)

8.47

Isoelectric Point (pI)

50.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-NF-X1 PF01422 446 - 463 6.5e-07 NF-X1 type zinc finger
RRM_NFXL1 PF24435 912 - 990 4.4e-42 NFXL1 RRM-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011120)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10170 AT1G10170 AT1G10170 AT1G10170
fragaria_vesca FvH4_5g17660
malus_domestica MD06G1232100.v1.1 MD14G1238900.v1.1
prunus_persica Prupe.5G236400_v2.0.a1
pyrus_communis pycom06g20620 pycom14g19990
rosa_chinensis RchiOBHm_Chr7g0178011
rosa_laevigata RLG00000005482
rosa_multiflora Rmu_sc0013399.1_g000001
rosa_roxburghii Rroxscaffold_3G00275290
rosa_rugosa Rorug06G0412300
rosa_samantha Rh7AG011800 Rh7BG011200 Rh7CG011800 Rh7DG011800
rosa_wichuraiana Rw7G000930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 3156
Acc65I GGTACC 2 cut(s) 772, 1748
AccB1I GGYRCC 4 cut(s) 82, 772, 1748, 1849
AccBSI CCGCTC 3 cut(s) 991, 2350, 2690
AccI GTMKAC 1 cut(s) 2760
AccII CGCG 1 cut(s) 3091
AcoI YGGCCR 4 cut(s) 302, 344, 1660, 2161
AcsI RAATTY 4 cut(s) 412, 435, 1472, 3298
AcuI CTGAAG 2 cut(s) 693, 3405
AcvI CACGTG 1 cut(s) 1143
AcyI GRCGYC 1 cut(s) 976
AfaI GTAC 9 cut(s) 645, 774, 1750, 2086, 2231, 2501, 2713, 3183, 3216
AfiI CCNNNNNNNGG 7 cut(s) 311, 361, 782, 1372, 1716, 2634, 2691
AflIII ACRYGT 4 cut(s) 588, 1142, 2081, 3165
AhdI GACNNNNNGTC 1 cut(s) 942
AjiI CACGTC 1 cut(s) 2459
AjnI CCWGG 7 cut(s) 38, 240, 628, 835, 1195, 2771, 3257
AleI CACNNNNGTG 2 cut(s) 816, 984
Alw21I GWGCWC 5 cut(s) 574, 2059, 2511, 2731, 2824
Alw26I GTCTC 4 cut(s) 1077, 1332, 1824, 2543
Alw44I GTGCAC 2 cut(s) 2727, 2820
AlwNI CAGNNNCTG 2 cut(s) 2177, 2915
Ama87I CYCGRG 4 cut(s) 567, 761, 1431, 2904
AoxI GGCC 6 cut(s) 302, 344, 1199, 1660, 2161, 2217
ApaLI GTGCAC 2 cut(s) 2727, 2820
ApeKI GCWGC 8 cut(s) 116, 1161, 1179, 1771, 1818, 2909, 3191, 3355
ApoI RAATTY 4 cut(s) 412, 435, 1472, 3298
Asp718I GGTACC 2 cut(s) 772, 1748
AspA2I CCTAGG 1 cut(s) 2060
AspLEI GCGC 3 cut(s) 2942, 2957, 3157
AspS9I GGNCC 9 cut(s) 36, 513, 839, 1007, 1199, 1696, 1885, 2446, 3290
AsuC2I CCSGG 1 cut(s) 777
AsuHPI GGTGA 9 cut(s) 160, 361, 502, 971, 1117, 1976, 2090, 2426, 3134
AvaI CYCGRG 4 cut(s) 567, 761, 1431, 2904
AvaII GGWCC 8 cut(s) 36, 513, 839, 1007, 1696, 1885, 2446, 3290
AvrII CCTAGG 1 cut(s) 2060
AxyI CCTNAGG 1 cut(s) 1889
BaeGI GKGCMC 3 cut(s) 461, 2731, 2824
BalI TGGCCA 1 cut(s) 1662
BamHI GGATCC 4 cut(s) 92, 1291, 1582, 2767
BanI GGYRCC 4 cut(s) 82, 772, 1748, 1849
BanII GRGCYC 4 cut(s) 568, 574, 2643, 3242
BbrPI CACGTG 1 cut(s) 1143
BbsI GAAGAC 2 cut(s) 1522, 1949
Bbv12I GWGCWC 5 cut(s) 574, 2059, 2511, 2731, 2824
BbvCI CCTCAGC 2 cut(s) 1788, 3059
BbvI GCAGC 8 cut(s) 103, 1148, 1191, 1758, 1805, 2921, 3178, 3367
BccI CCATC 8 cut(s) 298, 549, 1112, 1200, 2338, 2601, 3201, 3301
BceAI ACGGC 3 cut(s) 104, 1759, 3246
BcgI CGANNNNNNTGC 6 cut(s) 575, 609, 2858, 2892, 3309, 3343
BciT130I CCWGG 7 cut(s) 40, 242, 630, 837, 1197, 2773, 3259
BciVI GTATCC 1 cut(s) 2688
BcnI CCSGG 1 cut(s) 777
BcoDI GTCTC 4 cut(s) 1077, 1332, 1824, 2543
BfaI CTAG 5 cut(s) 2061, 2208, 2802, 3218, 3273
BfmI CTRYAG 4 cut(s) 1177, 2958, 3051, 3147
BfoI RGCGCY 1 cut(s) 2958
BfuI GTATCC 1 cut(s) 2688
BglI GCCNNNNNGGC 2 cut(s) 1495, 1798
BglII AGATCT 1 cut(s) 2794
BlnI CCTAGG 1 cut(s) 2060
BlpI GCTNAGC 3 cut(s) 51, 1157, 2011
BmcAI AGTACT 1 cut(s) 2501
Bme1390I CCNGG 8 cut(s) 40, 242, 630, 777, 837, 1197, 2773, 3259
Bme18I GGWCC 8 cut(s) 36, 513, 839, 1007, 1696, 1885, 2446, 3290
BmeRI GACNNNNNGTC 1 cut(s) 942
BmeT110I CYCGRG 4 cut(s) 567, 761, 1431, 2904
BmgBI CACGTC 1 cut(s) 2459
BmgT120I GGNCC 9 cut(s) 36, 513, 839, 1007, 1199, 1696, 1885, 2446, 3290
BmrFI CCNGG 8 cut(s) 40, 242, 630, 777, 837, 1197, 2773, 3259
BmrI ACTGGG 3 cut(s) 1380, 3032, 3406
BmuI ACTGGG 3 cut(s) 1380, 3032, 3406
BpiI GAAGAC 2 cut(s) 1522, 1949
BpmI CTGGAG 1 cut(s) 2724
Bpu10I CCTNAGC 3 cut(s) 1788, 3033, 3059
Bpu1102I GCTNAGC 3 cut(s) 51, 1157, 2011
BpuEI CTTGAG 2 cut(s) 505, 2525
BpuMI CCSGG 1 cut(s) 777
Bsa29I ATCGAT 1 cut(s) 3205
BsaAI YACGTR 1 cut(s) 1143
BsaBI GATNNNNATC 2 cut(s) 637, 1581
BsaHI GRCGYC 1 cut(s) 976
BsaI GGTCTC 1 cut(s) 2543
BsaJI CCNNGG 7 cut(s) 835, 1196, 2060, 2771, 3071, 3257, 3258
BsaWI WCCGGW 1 cut(s) 921
BsaXI ACNNNNNCTCC 4 cut(s) 656, 686, 2837, 2867
Bsc4I CCNNNNNNNGG 7 cut(s) 311, 361, 782, 1372, 1716, 2634, 2691
Bse118I RCCGGY 2 cut(s) 346, 2285
Bse1I ACTGG 5 cut(s) 1348, 1375, 3027, 3359, 3401
Bse21I CCTNAGG 1 cut(s) 1889
Bse3DI GCAATG 5 cut(s) 105, 169, 217, 265, 2925
Bse8I GATNNNNATC 2 cut(s) 637, 1581
BseBI CCWGG 7 cut(s) 40, 242, 630, 837, 1197, 2773, 3259
BseCI ATCGAT 1 cut(s) 3205
BseDI CCNNGG 7 cut(s) 835, 1196, 2060, 2771, 3071, 3257, 3258
BseGI GGATG 8 cut(s) 638, 1192, 1347, 2172, 2404, 2587, 2598, 3242
BseJI GATNNNNATC 2 cut(s) 637, 1581
BseLI CCNNNNNNNGG 7 cut(s) 311, 361, 782, 1372, 1716, 2634, 2691
BseMI GCAATG 5 cut(s) 105, 169, 217, 265, 2925
BseMII CTCAG 9 cut(s) 65, 74, 1584, 1802, 1903, 2025, 2424, 3050, 3210
BseNI ACTGG 5 cut(s) 1348, 1375, 3027, 3359, 3401
BseRI GAGGAG 7 cut(s) 327, 404, 508, 1722, 2376, 2501, 2938
BseSI GKGCMC 3 cut(s) 461, 2731, 2824
BseX3I CGGCCG 2 cut(s) 302, 344
BseXI GCAGC 8 cut(s) 103, 1148, 1191, 1758, 1805, 2921, 3178, 3367
BseYI CCCAGC 4 cut(s) 171, 420, 2733, 3350
BsgI GTGCAG 2 cut(s) 486, 1546
Bsh1236I CGCG 1 cut(s) 3091
Bsh1285I CGRYCG 3 cut(s) 305, 347, 922
BshFI GGCC 6 cut(s) 304, 346, 1201, 1662, 2163, 2219
BshNI GGYRCC 4 cut(s) 82, 772, 1748, 1849
BshVI ATCGAT 1 cut(s) 3205
BsiEI CGRYCG 3 cut(s) 305, 347, 922
BsiHKAI GWGCWC 5 cut(s) 574, 2059, 2511, 2731, 2824
BsiHKCI CYCGRG 4 cut(s) 567, 761, 1431, 2904
BsiSI CCGG 5 cut(s) 347, 776, 922, 2164, 2286
BslFI GGGAC 3 cut(s) 1527, 1928, 3040
BslI CCNNNNNNNGG 7 cut(s) 311, 361, 782, 1372, 1716, 2634, 2691
BsmAI GTCTC 4 cut(s) 1077, 1332, 1824, 2543
BsmFI GGGAC 3 cut(s) 1527, 1928, 3040
BsmI GAATGC 3 cut(s) 1448, 1633, 2881
BsnI GGCC 6 cut(s) 304, 346, 1201, 1662, 2163, 2219
Bso31I GGTCTC 1 cut(s) 2543
BsoBI CYCGRG 4 cut(s) 567, 761, 1431, 2904
Bsp1286I GDGCHC 9 cut(s) 461, 568, 574, 2059, 2511, 2643, 2731, 2824, 3242
Bsp1407I TGTACA 1 cut(s) 643
Bsp1720I GCTNAGC 3 cut(s) 51, 1157, 2011
BspANI GGCC 6 cut(s) 304, 346, 1201, 1662, 2163, 2219
BspCNI CTCAG 9 cut(s) 64, 73, 1583, 1801, 1902, 2024, 2425, 3051, 3209
BspDI ATCGAT 1 cut(s) 3205
BspFNI CGCG 1 cut(s) 3091
BspMAI CTGCAG 2 cut(s) 1181, 3055
BspT107I GGYRCC 4 cut(s) 82, 772, 1748, 1849
BspTNI GGTCTC 1 cut(s) 2543
BsrBI CCGCTC 3 cut(s) 991, 2350, 2690
BsrDI GCAATG 5 cut(s) 105, 169, 217, 265, 2925
BsrFI RCCGGY 2 cut(s) 346, 2285
BsrGI TGTACA 1 cut(s) 643
BsrI ACTGG 5 cut(s) 1348, 1375, 3027, 3359, 3401
BssAI RCCGGY 2 cut(s) 346, 2285
BssECI CCNNGG 7 cut(s) 835, 1196, 2060, 2771, 3071, 3257, 3258
BssNI GRCGYC 1 cut(s) 976
BssT1I CCWWGG 1 cut(s) 2060
Bst2UI CCWGG 7 cut(s) 40, 242, 630, 837, 1197, 2773, 3259
Bst6I CTCTTC 2 cut(s) 2570, 3123
BstACI GRCGYC 1 cut(s) 976
BstAPI GCANNNNNTGC 1 cut(s) 2915
BstAUI TGTACA 1 cut(s) 643
BstBAI YACGTR 1 cut(s) 1143
BstDSI CCRYGG 1 cut(s) 3071
BstENI CCTNNNNNAGG 1 cut(s) 1714
BstF5I GGATG 8 cut(s) 638, 1192, 1347, 2172, 2404, 2587, 2598, 3242
BstFNI CGCG 1 cut(s) 3091
BstH2I RGCGCY 1 cut(s) 2958
BstHHI GCGC 3 cut(s) 2942, 2957, 3157
BstMAI GTCTC 4 cut(s) 1077, 1332, 1824, 2543
BstMCI CGRYCG 3 cut(s) 305, 347, 922
BstNI CCWGG 7 cut(s) 40, 242, 630, 837, 1197, 2773, 3259
BstNSI RCATGY 5 cut(s) 592, 1245, 1883, 1967, 2085
BstSCI CCNGG 8 cut(s) 38, 240, 628, 775, 835, 1195, 2771, 3257
BstSFI CTRYAG 4 cut(s) 1177, 2958, 3051, 3147
BstSLI GKGCMC 3 cut(s) 461, 2731, 2824
BstUI CGCG 1 cut(s) 3091
BstV1I GCAGC 8 cut(s) 103, 1148, 1191, 1758, 1805, 2921, 3178, 3367
BstV2I GAAGAC 2 cut(s) 1522, 1949
BstX2I RGATCY 6 cut(s) 92, 1291, 1582, 2295, 2767, 2794
BstXI CCANNNNNNTGG 1 cut(s) 2975
BstYI RGATCY 6 cut(s) 92, 1291, 1582, 2295, 2767, 2794
BstZI CGGCCG 2 cut(s) 302, 344
Bsu15I ATCGAT 1 cut(s) 3205
Bsu36I CCTNAGG 1 cut(s) 1889
BsuI GTATCC 1 cut(s) 2688
BsuRI GGCC 6 cut(s) 304, 346, 1201, 1662, 2163, 2219
BsuTUI ATCGAT 1 cut(s) 3205
BtgI CCRYGG 1 cut(s) 3071
BtrI CACGTC 1 cut(s) 2459
BtsCI GGATG 8 cut(s) 638, 1192, 1347, 2172, 2404, 2587, 2598, 3242
BtsI GCAGTG 7 cut(s) 111, 833, 1443, 1534, 1663, 1958, 3060
CaiI CAGNNNCTG 2 cut(s) 2177, 2915
CfoI GCGC 3 cut(s) 2942, 2957, 3157
Cfr10I RCCGGY 2 cut(s) 346, 2285
Cfr13I GGNCC 9 cut(s) 36, 513, 839, 1007, 1199, 1696, 1885, 2446, 3290
ClaI ATCGAT 1 cut(s) 3205
CseI GACGC 3 cut(s) 965, 1029, 3036
Csp6I GTAC 9 cut(s) 644, 773, 1749, 2085, 2230, 2500, 2712, 3182, 3215
CspCI CAANNNNNGTGG 6 cut(s) 1371, 1406, 1474, 1509, 1918, 1953
CviQI GTAC 9 cut(s) 644, 773, 1749, 2085, 2230, 2500, 2712, 3182, 3215
DraI TTTAAA 1 cut(s) 1450
DriI GACNNNNNGTC 1 cut(s) 942
EaeI YGGCCR 4 cut(s) 302, 344, 1660, 2161
EagI CGGCCG 2 cut(s) 302, 344
Eam1104I CTCTTC 2 cut(s) 2570, 3123
Eam1105I GACNNNNNGTC 1 cut(s) 942
EarI CTCTTC 2 cut(s) 2570, 3123
Ecl136II GAGCTC 1 cut(s) 572
EclXI CGGCCG 2 cut(s) 302, 344
Eco130I CCWWGG 1 cut(s) 2060
Eco24I GRGCYC 4 cut(s) 568, 574, 2643, 3242
Eco31I GGTCTC 1 cut(s) 2543
Eco47I GGWCC 8 cut(s) 36, 513, 839, 1007, 1696, 1885, 2446, 3290
Eco52I CGGCCG 2 cut(s) 302, 344
Eco53kI GAGCTC 1 cut(s) 572
Eco57I CTGAAG 2 cut(s) 693, 3405
Eco72I CACGTG 1 cut(s) 1143
Eco81I CCTNAGG 1 cut(s) 1889
Eco88I CYCGRG 4 cut(s) 567, 761, 1431, 2904
EcoICRI GAGCTC 1 cut(s) 572
EcoNI CCTNNNNNAGG 1 cut(s) 1714
EcoO109I RGGNCCY 1 cut(s) 1007
EcoRII CCWGG 7 cut(s) 38, 240, 628, 835, 1195, 2771, 3257
EcoT14I CCWWGG 1 cut(s) 2060
EcoT22I ATGCAT 2 cut(s) 1965, 2881
EcoT38I GRGCYC 4 cut(s) 568, 574, 2643, 3242
ErhI CCWWGG 1 cut(s) 2060
FaqI GGGAC 3 cut(s) 1527, 1928, 3040
FauI CCCGC 5 cut(s) 135, 589, 743, 2307, 2695
FblI GTMKAC 1 cut(s) 2760
FokI GGATG 8 cut(s) 645, 1179, 1354, 2179, 2411, 2594, 2605, 3229
FriOI GRGCYC 4 cut(s) 568, 574, 2643, 3242
FspBI CTAG 5 cut(s) 2061, 2208, 2802, 3218, 3273
FspI TGCGCA 1 cut(s) 3156
GlaI GCGC 3 cut(s) 2941, 2956, 3156
GsaI CCCAGC 4 cut(s) 175, 424, 2737, 3354
GsuI CTGGAG 1 cut(s) 2724
HaeII RGCGCY 1 cut(s) 2958
HaeIII GGCC 6 cut(s) 304, 346, 1201, 1662, 2163, 2219
HapII CCGG 5 cut(s) 347, 776, 922, 2164, 2286
HgaI GACGC 3 cut(s) 965, 1029, 3036
HhaI GCGC 3 cut(s) 2942, 2957, 3157
Hin1I GRCGYC 1 cut(s) 976
Hin6I GCGC 3 cut(s) 2940, 2955, 3155
HinP1I GCGC 3 cut(s) 2940, 2955, 3155
HincII GTYRAC 1 cut(s) 1425
HindII GTYRAC 1 cut(s) 1425
HindIII AAGCTT 5 cut(s) 959, 1261, 2235, 2315, 2612
HpaII CCGG 5 cut(s) 347, 776, 922, 2164, 2286
HphI GGTGA 9 cut(s) 160, 361, 502, 971, 1117, 1976, 2090, 2426, 3134
Hpy99I CGWCG 1 cut(s) 75
HpyCH4IV ACGT 5 cut(s) 813, 874, 1142, 2458, 3165
HpySE526I ACGT 5 cut(s) 813, 874, 1142, 2458, 3165
Hsp92I GRCGYC 1 cut(s) 976
HspAI GCGC 3 cut(s) 2940, 2955, 3155
KpnI GGTACC 2 cut(s) 776, 1752
LmnI GCTCC 6 cut(s) 577, 2062, 2347, 2389, 2638, 3044
Lsp1109I GCAGC 8 cut(s) 103, 1148, 1191, 1758, 1805, 2921, 3178, 3367
MaeI CTAG 5 cut(s) 2061, 2208, 2802, 3218, 3273
MaeII ACGT 5 cut(s) 813, 874, 1142, 2458, 3165
MbiI CCGCTC 3 cut(s) 991, 2350, 2690
MfeI CAATTG 2 cut(s) 2274, 3186
MflI RGATCY 6 cut(s) 92, 1291, 1582, 2295, 2767, 2794
MhlI GDGCHC 9 cut(s) 461, 568, 574, 2059, 2511, 2643, 2731, 2824, 3242
MlsI TGGCCA 1 cut(s) 1662
MluNI TGGCCA 1 cut(s) 1662
MlyI GAGTC 4 cut(s) 401, 3104, 3263, 3320
MmeI TCCRAC 6 cut(s) 120, 942, 1325, 2910, 3083, 3343
Mox20I TGGCCA 1 cut(s) 1662
Mph1103I ATGCAT 2 cut(s) 1965, 2881
MscI TGGCCA 1 cut(s) 1662
MseI TTAA 6 cut(s) 615, 1220, 1449, 1619, 2706, 2831
Msp20I TGGCCA 1 cut(s) 1662
MspA1I CMGCKG 4 cut(s) 984, 1771, 1818, 2630
MspI CCGG 5 cut(s) 347, 776, 922, 2164, 2286
MspR9I CCNGG 8 cut(s) 40, 242, 630, 777, 837, 1197, 2773, 3259
MunI CAATTG 2 cut(s) 2274, 3186
Mva1269I GAATGC 3 cut(s) 1448, 1633, 2881
MvaI CCWGG 7 cut(s) 40, 242, 630, 837, 1197, 2773, 3259
MvnI CGCG 1 cut(s) 3091
NciI CCSGG 1 cut(s) 777
NmeAIII GCCGAG 2 cut(s) 111, 2490
NmuCI GTSAC 9 cut(s) 166, 977, 1399, 1481, 1652, 1982, 2096, 2140, 3224
NsbI TGCGCA 1 cut(s) 3156
NsiI ATGCAT 2 cut(s) 1965, 2881
NspI RCATGY 5 cut(s) 592, 1245, 1883, 1967, 2085
OliI CACNNNNGTG 2 cut(s) 816, 984
PaeI GCATGC 1 cut(s) 1967
PaeR7I CTCGAG 3 cut(s) 567, 761, 2904
PasI CCCWGGG 1 cut(s) 3258
PciI ACATGT 2 cut(s) 588, 2081
PctI GAATGC 3 cut(s) 1448, 1633, 2881
PflFI GACNNNGTC 1 cut(s) 339
PfoI TCCNGGA 1 cut(s) 628
PleI GAGTC 4 cut(s) 401, 3103, 3263, 3319
PmaCI CACGTG 1 cut(s) 1143
PmlI CACGTG 1 cut(s) 1143
PpsI GAGTC 4 cut(s) 401, 3103, 3263, 3319
Ppu21I YACGTR 1 cut(s) 1143
PpuMI RGGWCCY 1 cut(s) 1007
PscI ACATGT 2 cut(s) 588, 2081
Psp124BI GAGCTC 1 cut(s) 574
Psp5II RGGWCCY 1 cut(s) 1007
Psp6I CCWGG 7 cut(s) 38, 240, 628, 835, 1195, 2771, 3257
PspCI CACGTG 1 cut(s) 1143
PspFI CCCAGC 4 cut(s) 171, 420, 2733, 3350
PspGI CCWGG 7 cut(s) 38, 240, 628, 835, 1195, 2771, 3257
PspPI GGNCC 9 cut(s) 36, 513, 839, 1007, 1199, 1696, 1885, 2446, 3290
PspPPI RGGWCCY 1 cut(s) 1007
PspXI VCTCGAGB 2 cut(s) 567, 2904
PstI CTGCAG 2 cut(s) 1181, 3055
PstNI CAGNNNCTG 2 cut(s) 2177, 2915
PsuI RGATCY 6 cut(s) 92, 1291, 1582, 2295, 2767, 2794
PsyI GACNNNGTC 1 cut(s) 339
PvuII CAGCTG 2 cut(s) 1771, 1818
RsaI GTAC 9 cut(s) 645, 774, 1750, 2086, 2231, 2501, 2713, 3183, 3216
RsaNI GTAC 9 cut(s) 644, 773, 1749, 2085, 2230, 2500, 2712, 3182, 3215
SacI GAGCTC 1 cut(s) 574
SaqAI TTAA 6 cut(s) 615, 1220, 1449, 1619, 2706, 2831
Sau96I GGNCC 9 cut(s) 36, 513, 839, 1007, 1199, 1696, 1885, 2446, 3290
ScaI AGTACT 1 cut(s) 2501
SchI GAGTC 4 cut(s) 401, 3104, 3263, 3320
ScrFI CCNGG 8 cut(s) 40, 242, 630, 777, 837, 1197, 2773, 3259
SduI GDGCHC 9 cut(s) 461, 568, 574, 2059, 2511, 2643, 2731, 2824, 3242
SfcI CTRYAG 4 cut(s) 1177, 2958, 3051, 3147
Sfr274I CTCGAG 3 cut(s) 567, 761, 2904
SinI GGWCC 8 cut(s) 36, 513, 839, 1007, 1696, 1885, 2446, 3290
SlaI CTCGAG 3 cut(s) 567, 761, 2904
SmlI CTYRAG 5 cut(s) 520, 567, 761, 2540, 2904
SmoI CTYRAG 5 cut(s) 520, 567, 761, 2540, 2904
SphI GCATGC 1 cut(s) 1967
SspMI CTAG 5 cut(s) 2061, 2208, 2802, 3218, 3273
SstI GAGCTC 1 cut(s) 574
StyD4I CCNGG 8 cut(s) 38, 240, 628, 775, 835, 1195, 2771, 3257
StyI CCWWGG 1 cut(s) 2060
TaiI ACGT 5 cut(s) 816, 877, 1145, 2461, 3168
TatI WGTACW 4 cut(s) 643, 2084, 2499, 3214
TauI GCSGC 3 cut(s) 307, 2630, 2996
Tru1I TTAA 6 cut(s) 615, 1220, 1449, 1619, 2706, 2831
Tru9I TTAA 6 cut(s) 615, 1220, 1449, 1619, 2706, 2831
TseFI GTSAC 9 cut(s) 166, 977, 1399, 1481, 1652, 1982, 2096, 2140, 3224
TseI GCWGC 8 cut(s) 116, 1161, 1179, 1771, 1818, 2909, 3191, 3355
Tsp45I GTSAC 9 cut(s) 166, 977, 1399, 1481, 1652, 1982, 2096, 2140, 3224
TspDTI ATGAA 8 cut(s) 723, 1119, 2346, 2537, 2651, 2775, 3105, 3240
TspGWI ACGGA 2 cut(s) 1709, 3060
Tth111I GACNNNGTC 1 cut(s) 339
VneI GTGCAC 2 cut(s) 2727, 2820
VpaK11BI GGWCC 8 cut(s) 36, 513, 839, 1007, 1696, 1885, 2446, 3290
XagI CCTNNNNNAGG 1 cut(s) 1714
XapI RAATTY 4 cut(s) 412, 435, 1472, 3298
XbaI TCTAGA 1 cut(s) 3272
XceI RCATGY 5 cut(s) 592, 1245, 1883, 1967, 2085
XhoI CTCGAG 3 cut(s) 567, 761, 2904
XmaJI CCTAGG 1 cut(s) 2060
XmiI GTMKAC 1 cut(s) 2760
XspI CTAG 5 cut(s) 2061, 2208, 2802, 3218, 3273
ZrmI AGTACT 1 cut(s) 2501
Zsp2I ATGCAT 2 cut(s) 1965, 2881
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.