Rw7G000930

NF-X1-type zinc finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0011120
Physical Location & Seq
Reverse (-)
783026 .. 791587
8562 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G000930.1

Sequence Viewer

Length: 5808 bp
ATGGCTTCTCAGGTCCGAAGTGAGCGTAGGGAGAATAATAATAGGTCCAGGAATCCTGCTCAGCCTCGCTGGGTGCCCAGAGGATCCAACCCAACCCCCGCCGCCGCTGCCGTGAACCCGCCGAGCTTTGGTTCGAACCTACCCGATGGGAATGCAGGGATGCCCAGCAACAGCTCTGAAGCAGGAGAGGGCCGGCAGCATAGAGGAGGACATGCTGGAAATGGAAATGGAGGGCAGCAGAACCACATTTCTGCACCAGCAGAGAGTCGGCAGCATAGAGGAGGATATAGTGGAAATGGTGGGCAGCTCAATTATGTTCGGCTGCATAGAGGAGGACACAATGGGAATGGAGGGCAGTCCAATCACAGCTTTACATTATCGGAGAATCGGCAGCACAGAGGAGGACATAATGGCTCCAGGGGTAATGTGCTTCGGCCAATGAATAATAGAAGGGAGAGGGAAAGGAGTGAGAGTCAGGAGGGGATGGGCATGAAGGACTCTAGTTTGCCGCAGCTTGTGCAAGAAATTCAGGATAAACTGACTAAGGGCACTGTTGAGTGCATGATTTGTTATGACATGGTGAGGAGGTCTGCACCTGTATGGTCCTGCTCAAGCTGTTTCTCGATTTTCCATCTGAATTGCATCAAGAAATGGGCTCGAGCCCCCACTTCTATTGATATGTCTGCAGGAAAGAATCAGGGATTTAATTGGCGGTGTCCAGGATGTCAGTCTGTACAGCTCACATCGTCAAAGGAGATTCGCTACATGTGTTTTTGTGGTAAGCGGACAGACCCACCTTCGGATTTGTATTTGACACCACATTCATGTGGAGAACATTGTGGGAAGCCACTCGAGAAGGAGGTCCCAGGAAAGGGAATGAGTAAGGATGATCTTTGCCCTCATATGTGTGTCTTGCAGTGCCACCCAGGTCCATGCCCTCCTTGTAAAGCATTCGCACCGCCACGGTTATGCCCTTGTGGAAAGAAAACAATAACCACAAGATGCTCAGATCGGACTTCTGTTCTGACTTGTGGCAATCTTTGTAGTAAGCTTCTTGATTGTGGGCGTCACCGCTGTGAGCGGAAGTGCCATGTGGGTCCTTGTGATCCTTGTCAGGTTCCATTCAATGCTTCTTGCTTCTGCCTGAAAAATCTGGAGGTCGTTCTTTGTGGAGAGATGATAGTAAAGGGAGAGGTGAAAGCAGAAGATGGTGTTTTCTCTTGCAGTTCTAGTTGTGGAAAGAAGCTTAGCTGTGGCAATCATGTTTGCAGTGAAATTTGTCATCCAGGCCCTTGTGGAGAGTGCATTTTAATGCCACGGAATATCAAGACATGCCACTGTGGGAAAACAAGCTTGCAGGAGGAACGCCAGAGTTGTTTGGATCCGATTCCAACTTGTTCACAAATATGTGACAAGACTCTTCCTTGTGGGGTGCACCAGTGTCAACAGGTGTGCCATACTGGTGATTGCCCCCCTTGTTTGGTTAAAGTAACCCAAAAATGTCGCTGTGAATCAACTTCTCGGAATGTGGAGTGCTGTAAAACAACAATGGAGAATGAGAAATTTACTTGTGATAAGCCTTGTGGACGGAAGAAGAATTGTGGAAGGCACCGCTGCAGTGAAAGGTGCTGTCCTCTTTCTAATTCAAATAATCATCTTTCAGGGGATTGGGATCCTCACTTGTGCGGGCAGCATTCTTGTGAATCGCTGTGTCACAGTGGCCACTGTCCTCCCTGCCTTGATACAATCTTTACTGACTTGACATGTGCATGTGGGAGAACTTCAATCCCTCCTCCATTGCCCTGTGGTACACCTCCTCCATCATGTCAGCTCCCTTGTTCAGTGCCTCAGCCTTGTGGACATTCATCTTCTCACAGCTGCCACTTTGGAGACTGCCCACCTTGTTCAGTGCCTGTAGCAAAGGAGTGCATCGGTGGACATGTGGTCCTCAGGAACATTCCTTGTGGGTCGAGGGACATTAAATGTAACAAGCTCTGTGGGAAGATGAGGCAATGCGGTATGCATGCTTGCGGCAGAACATGTCACCCACCGCCTTGTGAGTGTTCCTCGTCGGCAGAAGCCAGCTCAAAATCTTCTTGTGGACAGAAATGTGGAGCACCTAGGAGAGATTGCAGGCATACATGTACTGCACCTTGTCACCCTTATGCTTCCTGTCCAGATGCGAGATGTGATTTCCTTGTCACAATTACTTGTTCTTGTGGCCGGATAATAGCAAATGTTCCTTGCGATTCTGGTGGCAGCACTGCTAGTTTTAATGCTGATACTGTGTTTGAAGCTTCCATTATCCAAAAGTTGCCAGTACCGCTTCAACCAGTTGAAGCCACCAACAAGAAGATCCCACTTGGACAGAGGAAGCTCATGTGTGATGATGAATGTGCTAAGTTGGAGCGGAAACGTGTTCTTGCAGATGCTTTTGATATAGTTCCTCCAAATTTGGATGCACTCCATTTTGGTGAGACTACTGTTATGTCTGAGTTGCTGTCAGACCTTTTCAGGCGTGATCCAAAATGGGTATTATCGGTGGAGGAGAGATGCAAACAATTGGTTCTTGGGAAGAACAAAGGTGCTACAAGTGGCCTCCGGGTCCATGTTTTTTGTCCAATGCTGAAGGAAAAGAGAGATGTGGTTAGGGTGATTGCTGACAGGTGGAAGCTTGCAGTTCAAGCTGCTGGCTGGGAGCCCAAGCGTTTTATTGTGGTACATGCTACACCTAAATCCAAAGTCCCAGCCAGGGTGCTTGGGGTAAAGGGTACCACCACAGTGAATGCATCCCAGCCTCCAGCTTTTGATCATTTAGTAGACATGGATCCCAGGCTTGTAGTTTCTTTTCCAGATCTTCCTAGAGATGCAGATATAAGTGCATTGGTCTTGAGGTTTGGTGGTGAATGTGAGCTAGTCTGGTTGAATGACAAGAACGCGTTGGCTGTATTCAATGATCCTGCTCGAGCTGCAACTGCAATGAGGAGGTTGGATAATGGTACACTCTATCATGGGGCTATTGCTGTTCTGTCTGCGGCTTCAGCTGGTTCTAATGCTTGGGGTGGAGGGGGAATAGCCAAGGAAGGAGCCTATACAGCACTGAAGGGTAATGCGTGGAAGAAGGCTGTTATTCGGGAGTCTTCTTGGAGAGAAGATTCATGGGGTGAGGAAGAGTGGTCTGGTGGTTCTGCAGATGTGCAGGCATCTGTGTGGAAAAAAGAAGCTCCAATTGCCGCCTCATTAAACCGCTGGAGTGTACTAGATAGTGAAGTGGCTTTGGGATCATCTTCTGTATCCCCCACAGTAGAGGACTCTGGAAAACAGACTGCTGCTCGTTTAATTTCCATGGTGGAATCAAATGCAAGTGGTTCAACTTCATCAAATGCAAGCGGTTCGACTTCAGCAGGGCAGCTTGGAGGAACTATTACAGAAACATCTGAAGTGGTAGATGACTGGGAGAAGGGGTTCCTACCTCAACCAACTCCAAACGCAAAGTCATTGATCATTTATAATCAGCTTGAGCTTATCATCTTGTGTAATATGCAGGATACATGGAAGCATACACTTCTTCTGTCATTTCAGAGCTTTGGAGTGATATATGGTCAAATGAGTACTGCTCCCTTATATGTCTTTGGGACAATTCATGCACAAGATATCAAATCGGAGGATACAGTTTATGAACTATTCTCCTTCATTTTCTGGACTATGACCATCATTACTTTACTGAAGTATGCCTGTATAGTATTAAGAGCTGATGATAATGGAGAAGGTGGTACTTTTGCTCTGTACTCACTCTTGTGTAGGCATGCCAAAGTGGGTCTGCTTCCAAATGACAGAAGTGCTGATGAGGTTATGCATTATGAGAGGGAAATTCCTTTCACGATGAAGGTGGAATCGAGGGCTAAAAGAACCATTGAAAAACATAAAAGTAGTCACTATGTCATGTTGGTCCTGGCTCTGTTAGGTTCCTGCATGACAATCGGCGTTGGGGTGCTTACCCCAGCTCTTTCGGTCTATTCAGCTTCATCAGGTGTTCAAAGATCACTGTCAGACATGGCCCACCGATTTTCATCTTCGGAAAGGAAACAAGAGTCAATAGCAAAAGCTTTCAAGGATTATGTACCAGTTCCCTTGGCATCTGCTATATTGGTGTGCCTCTTCATGCTGCAGCATTGTGGGACTAGTAAAATTGGGTTTATGTTTGCTCCGATTATCATTATTTGGCTGATATGTATCGGTGGCGTGGGTATATATAATATTTTCTACTGGGACAAACAAATTGTCCATGCAATGTCTCCTGTATACATGTACAGATTTGCAATGTTTGCAGATCTAGGTCATTTCAGAAAGAAATCCATCAAGATTATATTTGTATGCTTGATCTATCCTGTTCTTGTTCTATGCTATGCCGGTCAGGCTGCATTTATCTCCAAAAACTTTCATGAGGATGATTACAATCATCTCAGTGAATCCTTTCCTACACCAGTTCGCGACTGGTTCATAGCATTATCCCTACTTGCTTCAGCTGTAGGAAGTCAAGCTACCATAACAGCCAGTTTCTCCATCATAAATCAGTGCCTGGCACTTGGTTGTTTTCCCAGAGTAAAAGTCATTCATACATCAGATAAGATACATGGGCAGGTTTATATTCCCGATATCAACTGGATATTGATGGTTCTATGCCTTACTGTTACAATTGGTTTTCATGATATTGAGAAAATTGGCAATGCCACAGGTTTGGCCATAATTTCTCCAATGGTTGTCACAACTTGTCTCATGTCACTGGTAATTGCCTTATACTGGGAGAAGAGTTTGTATCAATCTATCTGTTTTCTGCTGTTCTTCGGCTTCATTGAGGCGATGTATGTGTCAGCTTGTATGTTGAATGTCCATAAGGGAGCTTGGTATTTGGTTTTCTTATTGGCATTGTCTTTGACTATCATGATTTCATGGCATTATGGAACCAAAAAGAAGCTCGAATTCGATTTAACAAACAAGGTGTCTGCAGAATGGCTCACAGCTATTAGCCCAGCACTAGGAGTTACCAGGGTACCTGGCATTGGTTTCATTTATACTGATATAGTCTCAGGAATTCCGGCTTTCTTCTCACACTTCATTACGAATCTTCCTGCATTTCATCAAGTTTTGATCTTTGTGTCCTTCAAGTCTCTGCCAGTTCCATATGTCCCTGCAACTCGGAGATACCTTGTGGGAAGGATCGGGCCAAAAGATCTTAAAATATATCGTTGTGTGGTGAGATATGGATATTGTGAGCATATTAGGGACACCGATGATTTTGAGGAAGAGATTATTGGTTCCATTGGAGAATTCATTTCCGTGGAAGAAAATGACTTTGATTCCCTGAATTCATCACAAGATAGAATGATCATAGTTGGCAAGCCACCATCAAATGGAAGTGCTTTGATTCCTTTAAATGAGATCACCTCATATGAGGACTCCGTGAGCTTGGTGCACAATGAAACCCGGTTAAGTACATTGGCCGATGCATGTGAGAGTGGTATGGTTTCGGTGCAGAGAAAAAAGGTTCGGTTCATGTTGCCTCCTAATAGTCCTAAGATGCGAGTATCTGTTAGGGAGGAGCTCCAAGAACTGATTGATGCTAGGGAGAGTGGAACTGCATATTTCTTGGGTCAGGTGCATTTGGCAGTTCGTGAAGGCTCAAACATATTCAAGCGGTTACTGATCATGACCTATGTTTTGAGTGAGAAGAATTGCAGAGAGCCTCCAGTTGCACTCAACATTCCTCACGCTGCTCTTGTTCAGGTTGGTATGGTCTGTAGCATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000122 GO:0000976 GO:0000977 GO:0000981 GO:0000982 GO:0001012 GO:0001067 GO:0001078 GO:0001227 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006082 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006970 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009416 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009636 GO:0009642 GO:0009651 GO:0009696 GO:0009697 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010188 GO:0010310 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0010817 GO:0016053 GO:0016070 GO:0016999 GO:0017000 GO:0017144 GO:0018130 GO:0018958 GO:0019219 GO:0019222 GO:0019438 GO:0019752 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032774 GO:0032787 GO:0034641 GO:0034645 GO:0034654 GO:0042221 GO:0042445 GO:0042446 GO:0042537 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0046189 GO:0046394 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051193 GO:0051252 GO:0051253 GO:0051704 GO:0051707 GO:0060255 GO:0065007 GO:0065008 GO:0071704 GO:0072330 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:0098542 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901615 GO:1901617 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:2000112 GO:2000113 GO:2000377 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko05165 map05165
Pfam Domains
Protein Families
K_trans R3H zf-NF-X1

Protein Analysis

1935

Amino Acids

212.06

Weight (kDa)

8.4

Isoelectric Point (pI)

47.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-NF-X1 PF01422 476 - 493 4.9e-06 NF-X1 type zinc finger
RRM_NFXL1 PF24435 932 - 1009 2.4e-42 NFXL1 RRM-like domain
K_trans PF02705 1192 - 1440 2.1e-53 K+ potassium transporter integral membrane domain
K_trans PF02705 1440 - 1673 8e-64 K+ potassium transporter integral membrane domain
K_trans_C PF22776 1689 - 1935 2.9e-22 K+ potassium transporter C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011120)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10170 AT1G10170 AT1G10170 AT1G10170
fragaria_vesca FvH4_5g17660
malus_domestica MD06G1232100.v1.1 MD14G1238900.v1.1
prunus_persica Prupe.5G236400_v2.0.a1
pyrus_communis pycom06g20620 pycom14g19990
rosa_chinensis RchiOBHm_Chr7g0178011
rosa_laevigata RLG00000005482
rosa_multiflora Rmu_sc0013399.1_g000001
rosa_roxburghii Rroxscaffold_3G00275290
rosa_rugosa Rorug06G0412300
rosa_samantha Rh7AG011800 Rh7BG011200 Rh7CG011800 Rh7DG011800
rosa_wichuraiana Rw7G000930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3510
Acc36I ACCTGC 1 cut(s) 4630
Acc65I GGTACC 2 cut(s) 2771, 5050
AccB1I GGYRCC 4 cut(s) 73, 1608, 2771, 5050
AccB7I CCANNNNNTGG 1 cut(s) 5411
AccBSI CCGCTC 2 cut(s) 1081, 2410
AccI GTMKAC 2 cut(s) 2820, 4302
AccII CGCG 2 cut(s) 2939, 4491
AcoI YGGCCR 5 cut(s) 434, 1720, 2221, 4740, 5499
AcsI RAATTY 9 cut(s) 525, 1275, 1562, 2452, 3870, 4979, 5091, 5327, 5365
AcuI CTGAAG 8 cut(s) 198, 2648, 3024, 3122, 3384, 3459, 3746, 4506
AcyI GRCGYC 1 cut(s) 1066
AflIII ACRYGT 8 cut(s) 765, 1763, 1939, 2039, 2141, 2416, 2937, 4305
AhdI GACNNNNNGTC 1 cut(s) 1943
AhlI ACTAGT 1 cut(s) 4181
AjuI GAANNNNNNNTTGG 2 cut(s) 5295, 5327
AleI CACNNNNGTG 3 cut(s) 1074, 2780, 3796
AloI GAACNNNNNNTCC 2 cut(s) 3449, 3481
Alw21I GWGCWC 4 cut(s) 1437, 2119, 5475, 5604
Alw26I GTCTC 6 cut(s) 1884, 2471, 4299, 4778, 5089, 5172
Alw44I GTGCAC 2 cut(s) 1433, 5471
AlwNI CAGNNNCTG 2 cut(s) 1913, 4579
Ama87I CYCGRG 3 cut(s) 657, 851, 2964
ApaLI GTGCAC 2 cut(s) 1433, 5471
ApoI RAATTY 9 cut(s) 525, 1275, 1562, 2452, 3870, 4979, 5091, 5327, 5365
ArsI GACNNNNNNTTYG 8 cut(s) 3479, 3511, 4266, 4298, 4985, 5017, 5701, 5733
Asp700I GAANNNNTTC 2 cut(s) 3464, 4473
Asp718I GGTACC 2 cut(s) 2771, 5050
AspA2I CCTAGG 1 cut(s) 2120
AsuC2I CCSGG 2 cut(s) 2603, 5485
AsuII TTCGAA 1 cut(s) 134
AvaI CYCGRG 3 cut(s) 657, 851, 2964
AvaII GGWCC 9 cut(s) 13, 45, 603, 862, 929, 1097, 1945, 2605, 3949
AvrII CCTAGG 1 cut(s) 2120
AxyI CCTNAGG 1 cut(s) 1949
BaeGI GKGCMC 4 cut(s) 78, 551, 1437, 5475
BaeI ACNNNNGTAYC 2 cut(s) 5034, 5067
BalI TGGCCA 2 cut(s) 1722, 4742
BamHI GGATCC 4 cut(s) 83, 1381, 1672, 2827
BanI GGYRCC 4 cut(s) 73, 1608, 2771, 5050
BanII GRGCYC 4 cut(s) 658, 664, 2703, 5604
BarI GAAGNNNNNNTAC 2 cut(s) 4525, 4557
BbsI GAAGAC 1 cut(s) 3132
Bbv12I GWGCWC 4 cut(s) 1437, 2119, 5475, 5604
BbvCI CCTCAGC 1 cut(s) 1848
BceAI ACGGC 1 cut(s) 95
BcgI CGANNNNNNTGC 6 cut(s) 134, 168, 3375, 3409, 3961, 3995
BciVI GTATCC 3 cut(s) 3304, 3541, 3661
BclI TGATCA 4 cut(s) 2809, 3501, 5385, 5703
BcnI CCSGG 2 cut(s) 2603, 5485
BcoDI GTCTC 6 cut(s) 1884, 2471, 4299, 4778, 5089, 5172
BcuI ACTAGT 1 cut(s) 4181
BfmI CTRYAG 8 cut(s) 684, 1615, 1914, 3189, 4166, 4527, 5004, 5797
BfuAI ACCTGC 1 cut(s) 4630
BfuI GTATCC 3 cut(s) 3304, 3541, 3661
BglI GCCNNNNNGGC 1 cut(s) 4415
BglII AGATCT 3 cut(s) 2854, 4330, 5230
BlnI CCTAGG 1 cut(s) 2120
BlpI GCTNAGC 2 cut(s) 60, 1247
BmcAI AGTACT 1 cut(s) 3613
Bme18I GGWCC 9 cut(s) 13, 45, 603, 862, 929, 1097, 1945, 2605, 3949
BmeRI GACNNNNNGTC 1 cut(s) 1943
BmeT110I CYCGRG 3 cut(s) 657, 851, 2964
BmrI ACTGGG 3 cut(s) 3463, 4276, 4810
BmuI ACTGGG 3 cut(s) 3463, 4276, 4810
BpiI GAAGAC 1 cut(s) 3132
BplI GAGNNNNNCTC 6 cut(s) 2051, 2083, 3601, 3633, 5429, 5461
BpmI CTGGAG 5 cut(s) 400, 1175, 2784, 3271, 5730
Bpu10I CCTNAGC 1 cut(s) 1848
Bpu1102I GCTNAGC 2 cut(s) 60, 1247
Bpu14I TTCGAA 1 cut(s) 134
BpuEI CTTGAG 3 cut(s) 595, 2911, 3539
BpuMI CCSGG 2 cut(s) 2603, 5485
BsaBI GATNNNNATC 4 cut(s) 1671, 4069, 4232, 4455
BsaHI GRCGYC 1 cut(s) 1066
BsaXI ACNNNNNCTCC 6 cut(s) 457, 487, 1801, 1831, 3449, 3479
Bse118I RCCGGY 2 cut(s) 192, 4409
Bse21I CCTNAGG 1 cut(s) 1949
Bse3DI GCAATG 6 cut(s) 1796, 2018, 2985, 4296, 4326, 4732
Bse8I GATNNNNATC 4 cut(s) 1671, 4069, 4232, 4455
BseGI GGATG 8 cut(s) 165, 489, 728, 892, 1282, 2464, 2789, 4453
BseJI GATNNNNATC 4 cut(s) 1671, 4069, 4232, 4455
BseMI GCAATG 6 cut(s) 1796, 2018, 2985, 4296, 4326, 4732
BseMII CTCAG 8 cut(s) 23, 74, 1020, 1862, 1963, 2484, 4477, 5100
BseSI GKGCMC 4 cut(s) 78, 551, 1437, 5475
BseYI CCCAGC 7 cut(s) 69, 164, 2694, 2746, 2793, 4000, 5029
BsgI GTGCAG 5 cut(s) 237, 576, 2133, 3218, 5552
Bsh1236I CGCG 2 cut(s) 2939, 4491
BshNI GGYRCC 4 cut(s) 73, 1608, 2771, 5050
BsiHKAI GWGCWC 4 cut(s) 1437, 2119, 5475, 5604
BsiHKCI CYCGRG 3 cut(s) 657, 851, 2964
BsiSI CCGG 6 cut(s) 193, 2224, 2602, 4410, 5096, 5485
BslFI GGGAC 8 cut(s) 848, 1988, 2729, 3649, 4192, 4283, 5171, 5297
BsmAI GTCTC 6 cut(s) 1884, 2471, 4299, 4778, 5089, 5172
BsmFI GGGAC 8 cut(s) 848, 1988, 2729, 3649, 4192, 4283, 5171, 5297
BsmI GAATGC 4 cut(s) 157, 950, 1693, 2791
BsoBI CYCGRG 3 cut(s) 657, 851, 2964
Bsp119I TTCGAA 1 cut(s) 134
Bsp1286I GDGCHC 9 cut(s) 78, 551, 658, 664, 1437, 2119, 2703, 5475, 5604
Bsp1407I TGTACA 2 cut(s) 733, 4308
Bsp1720I GCTNAGC 2 cut(s) 60, 1247
Bsp19I CCATGG 1 cut(s) 3345
Bsp68I TCGCGA 1 cut(s) 4491
BspCNI CTCAG 8 cut(s) 22, 73, 1019, 1861, 1962, 2485, 4476, 5099
BspFNI CGCG 2 cut(s) 2939, 4491
BspHI TCATGA 4 cut(s) 4441, 4705, 4941, 5706
BspMAI CTGCAG 5 cut(s) 688, 1619, 3193, 4170, 5008
BspMI ACCTGC 1 cut(s) 4630
BspT104I TTCGAA 1 cut(s) 134
BspT107I GGYRCC 4 cut(s) 73, 1608, 2771, 5050
BsrBI CCGCTC 2 cut(s) 1081, 2410
BsrDI GCAATG 6 cut(s) 1796, 2018, 2985, 4296, 4326, 4732
BsrFI RCCGGY 2 cut(s) 192, 4409
BsrGI TGTACA 2 cut(s) 733, 4308
BssAI RCCGGY 2 cut(s) 192, 4409
BssNAI GTATAC 1 cut(s) 4303
BssNI GRCGYC 1 cut(s) 1066
BssT1I CCWWGG 4 cut(s) 2120, 3078, 3345, 4131
Bst1107I GTATAC 1 cut(s) 4303
Bst6I CTCTTC 5 cut(s) 1425, 3165, 4163, 4802, 5298
BstACI GRCGYC 1 cut(s) 1066
BstAPI GCANNNNNTGC 2 cut(s) 517, 4325
BstAUI TGTACA 2 cut(s) 733, 4308
BstBI TTCGAA 1 cut(s) 134
BstDSI CCRYGG 4 cut(s) 962, 1316, 3345, 5337
BstF5I GGATG 8 cut(s) 165, 489, 728, 892, 1282, 2464, 2789, 4453
BstFNI CGCG 2 cut(s) 2939, 4491
BstMAI GTCTC 6 cut(s) 1884, 2471, 4299, 4778, 5089, 5172
BstSFI CTRYAG 8 cut(s) 684, 1615, 1914, 3189, 4166, 4527, 5004, 5797
BstSLI GKGCMC 4 cut(s) 78, 551, 1437, 5475
BstUI CGCG 2 cut(s) 2939, 4491
BstV2I GAAGAC 1 cut(s) 3132
BstX2I RGATCY 8 cut(s) 83, 1381, 1672, 2355, 2827, 2854, 4330, 5230
BstXI CCANNNNNNTGG 1 cut(s) 4738
BstYI RGATCY 8 cut(s) 83, 1381, 1672, 2355, 2827, 2854, 4330, 5230
BstZ17I GTATAC 1 cut(s) 4303
Bsu36I CCTNAGG 1 cut(s) 1949
BsuI GTATCC 3 cut(s) 3304, 3541, 3661
BtgI CCRYGG 4 cut(s) 962, 1316, 3345, 5337
BtgZI GCGATG 1 cut(s) 4874
BtsCI GGATG 8 cut(s) 165, 489, 728, 892, 1282, 2464, 2789, 4453
BtsI GCAGTG 4 cut(s) 923, 1276, 1624, 2262
BtuMI TCGCGA 1 cut(s) 4491
BveI ACCTGC 1 cut(s) 4630
CaiI CAGNNNCTG 2 cut(s) 1913, 4579
CciI TCATGA 4 cut(s) 4441, 4705, 4941, 5706
Cfr10I RCCGGY 2 cut(s) 192, 4409
CseI GACGC 1 cut(s) 1055
CspCI CAANNNNNGTGG 4 cut(s) 655, 690, 1978, 2013
DraI TTTAAA 1 cut(s) 5433
DriI GACNNNNNGTC 1 cut(s) 1943
EaeI YGGCCR 5 cut(s) 434, 1720, 2221, 4740, 5499
Eam1104I CTCTTC 5 cut(s) 1425, 3165, 4163, 4802, 5298
Eam1105I GACNNNNNGTC 1 cut(s) 1943
EarI CTCTTC 5 cut(s) 1425, 3165, 4163, 4802, 5298
Ecl136II GAGCTC 1 cut(s) 5602
Eco130I CCWWGG 4 cut(s) 2120, 3078, 3345, 4131
Eco24I GRGCYC 4 cut(s) 658, 664, 2703, 5604
Eco32I GATATC 2 cut(s) 3655, 4657
Eco47I GGWCC 9 cut(s) 13, 45, 603, 862, 929, 1097, 1945, 2605, 3949
Eco53kI GAGCTC 1 cut(s) 5602
Eco57I CTGAAG 8 cut(s) 198, 2648, 3024, 3122, 3384, 3459, 3746, 4506
Eco81I CCTNAGG 1 cut(s) 1949
Eco88I CYCGRG 3 cut(s) 657, 851, 2964
EcoICRI GAGCTC 1 cut(s) 5602
EcoO109I RGGNCCY 3 cut(s) 862, 1097, 1289
EcoRI GAATTC 4 cut(s) 4979, 5091, 5327, 5365
EcoRV GATATC 2 cut(s) 3655, 4657
EcoT14I CCWWGG 4 cut(s) 2120, 3078, 3345, 4131
EcoT22I ATGCAT 4 cut(s) 2025, 2791, 3858, 5509
EcoT38I GRGCYC 4 cut(s) 658, 664, 2703, 5604
ErhI CCWWGG 4 cut(s) 2120, 3078, 3345, 4131
FaqI GGGAC 8 cut(s) 848, 1988, 2729, 3649, 4192, 4283, 5171, 5297
FauI CCCGC 3 cut(s) 106, 126, 1679
FauNDI CATATG 3 cut(s) 903, 5182, 5449
FbaI TGATCA 4 cut(s) 2809, 3501, 5385, 5703
FblI GTMKAC 2 cut(s) 2820, 4302
FokI GGATG 8 cut(s) 172, 496, 735, 899, 1269, 2471, 2776, 4460
FriOI GRGCYC 4 cut(s) 658, 664, 2703, 5604
GsaI CCCAGC 7 cut(s) 73, 168, 2698, 2750, 2797, 4004, 5033
GsuI CTGGAG 5 cut(s) 400, 1175, 2784, 3271, 5730
HapII CCGG 6 cut(s) 193, 2224, 2602, 4410, 5096, 5485
HgaI GACGC 1 cut(s) 1055
Hin1I GRCGYC 1 cut(s) 1066
HincII GTYRAC 1 cut(s) 1445
HindII GTYRAC 1 cut(s) 1445
HindIII AAGCTT 6 cut(s) 1049, 1244, 1351, 2295, 2672, 4104
HpaII CCGG 6 cut(s) 193, 2224, 2602, 4410, 5096, 5485
Hpy99I CGWCG 1 cut(s) 2074
HpyCH4IV ACGT 1 cut(s) 2416
HpySE526I ACGT 1 cut(s) 2416
Hsp92I GRCGYC 1 cut(s) 1066
KpnI GGTACC 2 cut(s) 2775, 5054
KroI GCCGGC 1 cut(s) 192
KroNI GCCGGC 1 cut(s) 194
Ksp22I TGATCA 4 cut(s) 2809, 3501, 5385, 5703
MaeII ACGT 1 cut(s) 2416
MbiI CCGCTC 2 cut(s) 1081, 2410
MfeI CAATTG 3 cut(s) 2561, 3228, 4695
MflI RGATCY 8 cut(s) 83, 1381, 1672, 2355, 2827, 2854, 4330, 5230
MhlI GDGCHC 9 cut(s) 78, 551, 658, 664, 1437, 2119, 2703, 5475, 5604
MlsI TGGCCA 2 cut(s) 1722, 4742
MluI ACGCGT 1 cut(s) 2937
MluNI TGGCCA 2 cut(s) 1722, 4742
MlyI GAGTC 8 cut(s) 274, 481, 491, 1411, 3146, 3305, 4100, 5450
MmeI TCCRAC 4 cut(s) 111, 1415, 2385, 2970
Mox20I TGGCCA 2 cut(s) 1722, 4742
Mph1103I ATGCAT 4 cut(s) 2025, 2791, 3858, 5509
MroNI GCCGGC 1 cut(s) 192
MroXI GAANNNNTTC 2 cut(s) 3464, 4473
MscI TGGCCA 2 cut(s) 1722, 4742
Msp20I TGGCCA 2 cut(s) 1722, 4742
MspA1I CMGCKG 7 cut(s) 107, 1074, 1614, 1878, 3044, 3249, 4526
MspI CCGG 6 cut(s) 193, 2224, 2602, 4410, 5096, 5485
MunI CAATTG 3 cut(s) 2561, 3228, 4695
Mva1269I GAATGC 4 cut(s) 157, 950, 1693, 2791
MvnI CGCG 2 cut(s) 2939, 4491
NaeI GCCGGC 1 cut(s) 194
NciI CCSGG 2 cut(s) 2603, 5485
NcoI CCATGG 1 cut(s) 3345
NdeI CATATG 3 cut(s) 903, 5182, 5449
NgoMIV GCCGGC 1 cut(s) 192
NmeAIII GCCGAG 1 cut(s) 147
NmuCI GTSAC 9 cut(s) 1067, 1409, 1712, 2042, 2156, 2200, 3932, 4762, 4779
NruI TCGCGA 1 cut(s) 4491
NsiI ATGCAT 4 cut(s) 2025, 2791, 3858, 5509
NspV TTCGAA 1 cut(s) 134
OliI CACNNNNGTG 3 cut(s) 1074, 2780, 3796
PaeI GCATGC 2 cut(s) 2027, 3809
PaeR7I CTCGAG 3 cut(s) 657, 851, 2964
PagI TCATGA 4 cut(s) 4441, 4705, 4941, 5706
PciI ACATGT 6 cut(s) 765, 1763, 1939, 2039, 2141, 4305
PctI GAATGC 4 cut(s) 157, 950, 1693, 2791
PdiI GCCGGC 1 cut(s) 194
PdmI GAANNNNTTC 2 cut(s) 3464, 4473
PflMI CCANNNNNTGG 1 cut(s) 5411
PfoI TCCNGGA 2 cut(s) 47, 718
PleI GAGTC 8 cut(s) 273, 480, 491, 1411, 3145, 3305, 4099, 5450
PpsI GAGTC 8 cut(s) 273, 480, 491, 1411, 3145, 3305, 4099, 5450
PpuMI RGGWCCY 2 cut(s) 862, 1097
PscI ACATGT 6 cut(s) 765, 1763, 1939, 2039, 2141, 4305
PsiI TTATAA 1 cut(s) 3510
Psp124BI GAGCTC 1 cut(s) 5604
Psp5II RGGWCCY 2 cut(s) 862, 1097
PspFI CCCAGC 7 cut(s) 69, 164, 2694, 2746, 2793, 4000, 5029
PspPPI RGGWCCY 2 cut(s) 862, 1097
PspXI VCTCGAGB 2 cut(s) 657, 2964
PstI CTGCAG 5 cut(s) 688, 1619, 3193, 4170, 5008
PstNI CAGNNNCTG 2 cut(s) 1913, 4579
PsuI RGATCY 8 cut(s) 83, 1381, 1672, 2355, 2827, 2854, 4330, 5230
PvuII CAGCTG 3 cut(s) 1878, 3044, 4526
RruI TCGCGA 1 cut(s) 4491
SacI GAGCTC 1 cut(s) 5604
ScaI AGTACT 1 cut(s) 3613
SchI GAGTC 8 cut(s) 274, 481, 491, 1411, 3146, 3305, 4100, 5450
SduI GDGCHC 9 cut(s) 78, 551, 658, 664, 1437, 2119, 2703, 5475, 5604
SfcI CTRYAG 8 cut(s) 684, 1615, 1914, 3189, 4166, 4527, 5004, 5797
Sfr274I CTCGAG 3 cut(s) 657, 851, 2964
SfuI TTCGAA 1 cut(s) 134
SinI GGWCC 9 cut(s) 13, 45, 603, 862, 929, 1097, 1945, 2605, 3949
SlaI CTCGAG 3 cut(s) 657, 851, 2964
SmlI CTYRAG 6 cut(s) 610, 657, 851, 2890, 2964, 3518
SmoI CTYRAG 6 cut(s) 610, 657, 851, 2890, 2964, 3518
SpeI ACTAGT 1 cut(s) 4181
SphI GCATGC 2 cut(s) 2027, 3809
SspI AATATT 1 cut(s) 4258
SstI GAGCTC 1 cut(s) 5604
StyI CCWWGG 4 cut(s) 2120, 3078, 3345, 4131
TaiI ACGT 1 cut(s) 2419
TaqII GACCGA 1 cut(s) 4000
TatI WGTACW 7 cut(s) 733, 2144, 3256, 3611, 3786, 4308, 5492
TauI GCSGC 6 cut(s) 104, 107, 511, 2034, 3038, 3236
TseFI GTSAC 9 cut(s) 1067, 1409, 1712, 2042, 2156, 2200, 3932, 4762, 4779
Tsp45I GTSAC 9 cut(s) 1067, 1409, 1712, 2042, 2156, 2200, 3932, 4762, 4779
TspGWI ACGGA 4 cut(s) 1333, 1603, 5326, 5449
Van91I CCANNNNNTGG 1 cut(s) 5411
VneI GTGCAC 2 cut(s) 1433, 5471
VpaK11BI GGWCC 9 cut(s) 13, 45, 603, 862, 929, 1097, 1945, 2605, 3949
XapI RAATTY 9 cut(s) 525, 1275, 1562, 2452, 3870, 4979, 5091, 5327, 5365
XcmI CCANNNNNNNNNTGG 1 cut(s) 4491
XhoI CTCGAG 3 cut(s) 657, 851, 2964
XmaJI CCTAGG 1 cut(s) 2120
XmiI GTMKAC 2 cut(s) 2820, 4302
XmnI GAANNNNTTC 2 cut(s) 3464, 4473
ZrmI AGTACT 1 cut(s) 3613
Zsp2I ATGCAT 4 cut(s) 2025, 2791, 3858, 5509
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.