pycom06g20620

NF-X1-type zinc finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0011120
Physical Location & Seq
Forward (+)
23568765 .. 23571101
2337 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g20620.1

Sequence Viewer

Length: 2337 bp
ATGACTGTTAAGGGAGAGGTGAAAGCAGAAGATGGTGTTTTTTCATGCAGTTCCCCTTGTGGAAAAATGCTTAGTTGCGGTAATCATTCCTGCAGTGAGGTTTGCCATCCAGGGCCGTGCGGAGATTGCAATTTAATGCCAATTAAGATTAAGACATGCCACTGTGGGAAGACAAGCTTGCAAGAGGAACGACGGAGTTGTTTGGATCCGATTCCAACCTGTTCTCAATTATGTAGCAAGTCCCTCCCTTGTGAGATGCACCAGTGTCAAGAGGTGTGCCATACTGGGGATTGCCCACCTTGTTTGGTTGAAGTGACCCAAAAGTGTCGTTGTGGTTCAACTTCTCGGTCTGCGGAATGCTTTAAAACCACAATGGAGAATGAGAAATTCACTTGTGATAAGCCTTGTGGGCGGAAGAAGAATTGTGGAAGGCACCGGTGCAGCGAGAGGTGCTGTCCTCTTTCTAATTTGAATAATGCTCTCTTGGGGGATTGGGATCCTCACTTTTGCTCTATGTCCTGTGGGAAAAAGTTAAGGTGTGGTCAGCATTCTTGTGAATCGCTTTGTCACAGTGGCCACTGCCCTCCTTGCCTTGATACAATCTTCACGGACTTGACCTGTGCATGTGGGAGGACTTCAATCCCTCCTCCACTGCCATGCGGTACCCCTCCTCCATCATGTCAGCTCCCGTGTTCAGTCCCTCAGCCTTGTGGGCATACATCTTCTCACAGCTGCCACTTTGGAGACTGTCCACCATGTTCGGTGCCTGTGGCAAAGGAGTGCATTGGTGGGCATGTGGTCCTCAGGAACATTCCTTGTGGGTCGAGGGACATCAAATGTAACAAGCTCTGTGGGAAGACAAGGCAGTGTGGTATGCATGCTTGTGGCAGGACTTGTCACCCACCTCCCTGTGATACATCTTGTTTGGCAGAACAAGGTTCAAAAACTTCTTGTGGACAGATATGTGGTGCTCCTAGGAGAGATTGCAGGCATACATGTACTTCACTTTGTCACCCTTATGCTTCTTGTCCTGATAGCAGATGTGATTTTCCTGTCACAATCACTTGTTCTTGTGGCCGGATGACAGCAAGTGTTCCTTGTGATTCCGGTGGCAGCAATGCTAGTTTCAAGGCTGATATTGTTTATGAAGCTTCCATTGTTCAAAGGTTGCCAGCACCACTGCAACCAATTGAATCAACCTGCAAGAATATCCTGCTCGGACAGAGGAAGCTTATGTGTGATGATGAATGTGCTAAGAGGGAGCGGAAACGGGTTCTTGCAGATGCTTTTGATATAACTCCTCCAAATTTGGATGCGCTCCATTTTGGTGAGAGTTCTGCTGTTTCTGAGTTGCTTTCGGACCTTCTTAGACGCGATCCCAAGTGGGTATTATCTGTGGAGGAGAGATGCAAGTACTTGGTGCTCGGCAAGAGTAGACGTGCTACAAGTGGTCTCAAGGTTCATGTTTTCTGCCCGATGCTGAAGGAGAAGAGGGATGTAGTTCGGATGATCGATCCTGAAAGATGGAAGCTTGCAGTTCAAGCCGCTGGTTGGGAGCCCAAGCGATTTATTGTGGTTCATGTTACACCTAAATCCAAAGCCCCCGCTCGGATACTTGGGGTTAAGGGCACAATAACAGTGAGTGCACCCCAGCCTCCGGCTTATGAACATTTAGTGGACATGGATCCGAGGCTTGTAGTTTCATTCCCAGATCTCCCTAGAGATGCAGATATAAGTGCACTTGTATTGAGGTTTGGTGGGGAGTGTGAGTTAGTCTGGTTGAACGATAAGAATGCATTAGCTGTATTCAATGATCCTGCTCGAGCGGCAACTGCAAGGAGGAGGTTGGATAATGGTGCACTATATCATGGCGCTATTGCTGTTCACTCCAACGGCAGTGCATCTATGGCGGCATCAGGTTCTAATGCTTGGGGTGGATTGGGGACATCTAGGGAAGGAGGAGCTTCTGCAGTGCTGATGGGTAATCCATGGAAGAAGACTGTTACACAAGAGTCCGGTTGGAGGGAAGATTCATGGGGCAAGGAAGAGTGGCCTGGCAGTTCTACAGATGCGCCAGCAAACGTGTGGAATAAAAAAGCACCAATTGCTGCCTCAGTAAATCGATGGAGTGTACTGGACGGTGACACGGCATTGGGCTCATCCGCTTCATCTCCCAGAGTTGAGGAATCTAGAAAACAGTCTTTGGGTCCATTGAATTCGGCCTTGGACTTGAAAGCAAGTGGTTCGAGTTCGAGTTCCACTTTGGAAGGGCGGCCTGTTGGTGTTATTGCAGAAACACCTGAAGTGGTTGATGACTGGGAGAAGGTTTATGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000122 GO:0000976 GO:0000977 GO:0000981 GO:0000982 GO:0001012 GO:0001067 GO:0001078 GO:0001227 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006082 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006970 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009416 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009636 GO:0009642 GO:0009651 GO:0009696 GO:0009697 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010188 GO:0010310 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0010817 GO:0016053 GO:0016070 GO:0016999 GO:0017000 GO:0017144 GO:0018130 GO:0018958 GO:0019219 GO:0019222 GO:0019438 GO:0019752 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032774 GO:0032787 GO:0034641 GO:0034645 GO:0034654 GO:0042221 GO:0042445 GO:0042446 GO:0042537 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0046189 GO:0046394 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051193 GO:0051252 GO:0051253 GO:0051704 GO:0051707 GO:0060255 GO:0065007 GO:0065008 GO:0071704 GO:0072330 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:0098542 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901615 GO:1901617 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:2000112 GO:2000113 GO:2000377 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko05165 map05165
Pfam Domains
Protein Families
K_trans R3H zf-NF-X1

Protein Analysis

779

Amino Acids

83.9

Weight (kDa)

8.16

Isoelectric Point (pI)

56.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_NFXL1 PF24435 551 - 629 8.6e-40 NFXL1 RRM-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011120)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10170 AT1G10170 AT1G10170 AT1G10170
fragaria_vesca FvH4_5g17660
malus_domestica MD06G1232100.v1.1 MD14G1238900.v1.1
prunus_persica Prupe.5G236400_v2.0.a1
pyrus_communis pycom06g20620 pycom14g19990
rosa_chinensis RchiOBHm_Chr7g0178011
rosa_laevigata RLG00000005482
rosa_multiflora Rmu_sc0013399.1_g000001
rosa_roxburghii Rroxscaffold_3G00275290
rosa_rugosa Rorug06G0412300
rosa_samantha Rh7AG011800 Rh7BG011200 Rh7CG011800 Rh7DG011800
rosa_wichuraiana Rw7G000930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1208
Acc65I GGTACC 1 cut(s) 662
AccB1I GGYRCC 3 cut(s) 432, 662, 763
AccBSI CCGCTC 3 cut(s) 1264, 1607, 1826
AccI GTMKAC 1 cut(s) 1435
AccII CGCG 1 cut(s) 1374
AclWI GGATC 9 cut(s) 200, 213, 491, 504, 1370, 1508, 1679, 1692, 1808
AcoI YGGCCR 2 cut(s) 574, 1075
AcsI RAATTY 3 cut(s) 386, 1306, 2215
AcuI CTGAAG 2 cut(s) 1502, 2322
AfaI GTAC 4 cut(s) 664, 1000, 1415, 2133
AfiI CCNNNNNNNGG 5 cut(s) 286, 1551, 1608, 1657, 2022
AflIII ACRYGT 2 cut(s) 995, 2082
AgeI ACCGGT 1 cut(s) 435
AjiI CACGTC 1 cut(s) 1439
AjnI CCWGG 2 cut(s) 109, 2053
AjuI GAANNNNNNNTTGG 2 cut(s) 2207, 2239
AluBI AGCT 9 cut(s) 177, 685, 732, 847, 1151, 1231, 1531, 1802, 1964
AluI AGCT 9 cut(s) 177, 685, 732, 847, 1151, 1231, 1531, 1802, 1964
Alw21I GWGCWC 5 cut(s) 973, 1425, 1648, 1741, 1861
Alw26I GTCTC 2 cut(s) 738, 1457
Alw44I GTGCAC 3 cut(s) 1644, 1737, 1857
AlwI GGATC 9 cut(s) 200, 213, 491, 504, 1370, 1508, 1679, 1692, 1808
Ama87I CYCGRG 1 cut(s) 1821
AoxI GGCC 6 cut(s) 113, 574, 1075, 2051, 2220, 2273
ApaLI GTGCAC 3 cut(s) 1644, 1737, 1857
ApeKI GCWGC 4 cut(s) 441, 732, 1113, 2108
ApoI RAATTY 3 cut(s) 386, 1306, 2215
AsiGI ACCGGT 1 cut(s) 435
Asp718I GGTACC 1 cut(s) 662
AspA2I CCTAGG 1 cut(s) 974
AspLEI GCGC 3 cut(s) 1318, 1874, 2074
AspS9I GGNCC 4 cut(s) 113, 799, 1360, 2207
AsuHPI GGTGA 5 cut(s) 31, 890, 1004, 1340, 2153
AvaI CYCGRG 1 cut(s) 1821
AvaII GGWCC 3 cut(s) 799, 1360, 2207
AvrII CCTAGG 1 cut(s) 974
AxyI CCTNAGG 1 cut(s) 803
BaeGI GKGCMC 4 cut(s) 1631, 1648, 1741, 1861
BalI TGGCCA 1 cut(s) 576
BamHI GGATCC 3 cut(s) 205, 496, 1684
BanI GGYRCC 3 cut(s) 432, 662, 763
BanII GRGCYC 2 cut(s) 1560, 2159
BbsI GAAGAC 3 cut(s) 176, 863, 2003
Bbv12I GWGCWC 5 cut(s) 973, 1425, 1648, 1741, 1861
BbvCI CCTCAGC 1 cut(s) 702
BbvI GCAGC 4 cut(s) 453, 719, 1125, 2095
BccI CCATC 6 cut(s) 26, 114, 682, 1518, 1972, 2118
BceAI ACGGC 3 cut(s) 100, 1909, 2163
BcgI CGANNNNNNTGC 4 cut(s) 1775, 1809, 2226, 2260
BciT130I CCWGG 2 cut(s) 111, 2055
BciVI GTATCC 1 cut(s) 1605
BcoDI GTCTC 2 cut(s) 738, 1457
BfaI CTAG 5 cut(s) 975, 1122, 1719, 1950, 2190
BfmI CTRYAG 3 cut(s) 91, 1968, 2064
BfoI RGCGCY 1 cut(s) 1875
BfuAI ACCTGC 1 cut(s) 1208
BfuI GTATCC 1 cut(s) 1605
BglI GCCNNNNNGGC 2 cut(s) 409, 712
BglII AGATCT 1 cut(s) 1711
BisI GCNGC 8 cut(s) 442, 733, 1114, 1545, 1827, 1911, 2109, 2273
BlnI CCTAGG 1 cut(s) 974
BlsI GCNGC 8 cut(s) 443, 734, 1115, 1546, 1828, 1912, 2110, 2274
BmcAI AGTACT 1 cut(s) 1415
Bme1390I CCNGG 2 cut(s) 111, 2055
Bme18I GGWCC 3 cut(s) 799, 1360, 2207
BmeT110I CYCGRG 1 cut(s) 1821
BmgBI CACGTC 1 cut(s) 1439
BmgT120I GGNCC 4 cut(s) 113, 799, 1360, 2207
BmiI GGNNCC 8 cut(s) 207, 434, 498, 664, 765, 1557, 1686, 2208
BmrFI CCNGG 2 cut(s) 111, 2055
BmrI ACTGGG 2 cut(s) 294, 2326
BmsI GCATC 9 cut(s) 246, 1273, 1303, 1397, 1467, 1714, 1910, 1922, 2059
BmuI ACTGGG 2 cut(s) 294, 2326
BpiI GAAGAC 3 cut(s) 176, 863, 2003
Bpu10I CCTNAGC 1 cut(s) 702
BpuEI CTTGAG 1 cut(s) 1439
Bsa29I ATCGAT 2 cut(s) 1512, 2122
BsaBI GATNNNNATC 1 cut(s) 495
BsaI GGTCTC 1 cut(s) 1457
BsaJI CCNNGG 5 cut(s) 110, 974, 1688, 1988, 2223
BsaWI WCCGGW 3 cut(s) 435, 1106, 2015
BsaXI ACNNNNNCTCC 4 cut(s) 655, 685, 1754, 1784
Bsc4I CCNNNNNNNGG 5 cut(s) 286, 1551, 1608, 1657, 2022
Bse118I RCCGGY 1 cut(s) 435
Bse1I ACTGG 4 cut(s) 262, 289, 2139, 2321
Bse21I CCTNAGG 1 cut(s) 803
Bse3DI GCAATG 1 cut(s) 1123
Bse8I GATNNNNATC 1 cut(s) 495
BseBI CCWGG 2 cut(s) 111, 2055
BseCI ATCGAT 2 cut(s) 1512, 2122
BseDI CCNNGG 5 cut(s) 110, 974, 1688, 1988, 2223
BseGI GGATG 6 cut(s) 106, 1086, 1318, 1501, 1512, 2159
BseJI GATNNNNATC 1 cut(s) 495
BseLI CCNNNNNNNGG 5 cut(s) 286, 1551, 1608, 1657, 2022
BseMI GCAATG 1 cut(s) 1123
BseMII CTCAG 4 cut(s) 716, 817, 1338, 2127
BseNI ACTGG 4 cut(s) 262, 289, 2139, 2321
BseRI GAGGAG 6 cut(s) 636, 660, 1290, 1415, 1855, 1974
BseSI GKGCMC 4 cut(s) 1631, 1648, 1741, 1861
BseXI GCAGC 4 cut(s) 453, 719, 1125, 2095
BseYI CCCAGC 1 cut(s) 1650
BsgI GTGCAG 1 cut(s) 460
Bsh1236I CGCG 1 cut(s) 1374
BshFI GGCC 6 cut(s) 115, 576, 1077, 2053, 2222, 2275
BshNI GGYRCC 3 cut(s) 432, 662, 763
BshTI ACCGGT 1 cut(s) 435
BshVI ATCGAT 2 cut(s) 1512, 2122
BsiHKAI GWGCWC 5 cut(s) 973, 1425, 1648, 1741, 1861
BsiHKCI CYCGRG 1 cut(s) 1821
BsiSI CCGG 5 cut(s) 436, 1078, 1107, 1658, 2016
BslFI GGGAC 4 cut(s) 226, 683, 842, 1957
BslI CCNNNNNNNGG 5 cut(s) 286, 1551, 1608, 1657, 2022
BsmAI GTCTC 2 cut(s) 738, 1457
BsmFI GGGAC 4 cut(s) 226, 683, 842, 1957
BsmI GAATGC 3 cut(s) 362, 547, 1798
BsnI GGCC 6 cut(s) 115, 576, 1077, 2053, 2222, 2275
Bso31I GGTCTC 1 cut(s) 1457
BsoBI CYCGRG 1 cut(s) 1821
Bsp1286I GDGCHC 8 cut(s) 973, 1425, 1560, 1631, 1648, 1741, 1861, 2159
Bsp143I GATC 8 cut(s) 205, 496, 1375, 1509, 1513, 1684, 1711, 1813
Bsp19I CCATGG 1 cut(s) 1988
BspANI GGCC 6 cut(s) 115, 576, 1077, 2053, 2222, 2275
BspCNI CTCAG 4 cut(s) 715, 816, 1339, 2126
BspDI ATCGAT 2 cut(s) 1512, 2122
BspFNI CGCG 1 cut(s) 1374
BspLI GGNNCC 8 cut(s) 207, 434, 498, 664, 765, 1557, 1686, 2208
BspMAI CTGCAG 2 cut(s) 95, 1972
BspMI ACCTGC 1 cut(s) 1208
BspPI GGATC 9 cut(s) 200, 213, 491, 504, 1370, 1508, 1679, 1692, 1808
BspT107I GGYRCC 3 cut(s) 432, 662, 763
BspTNI GGTCTC 1 cut(s) 1457
BsrBI CCGCTC 3 cut(s) 1264, 1607, 1826
BsrDI GCAATG 1 cut(s) 1123
BsrFI RCCGGY 1 cut(s) 435
BsrI ACTGG 4 cut(s) 262, 289, 2139, 2321
BssAI RCCGGY 1 cut(s) 435
BssECI CCNNGG 5 cut(s) 110, 974, 1688, 1988, 2223
BssMI GATC 8 cut(s) 205, 496, 1375, 1509, 1513, 1684, 1711, 1813
BssT1I CCWWGG 3 cut(s) 974, 1988, 2223
Bst2UI CCWGG 2 cut(s) 111, 2055
Bst4CI ACNGT 8 cut(s) 7, 164, 572, 749, 1639, 2002, 2141, 2199
Bst6I CTCTTC 2 cut(s) 1484, 2040
BstAPI GCANNNNNTGC 1 cut(s) 2105
BstC8I GCNNGC 6 cut(s) 179, 879, 989, 1173, 1533, 2076
BstDEI CTNAG 7 cut(s) 71, 702, 803, 1254, 1347, 1367, 2113
BstDSI CCRYGG 1 cut(s) 1988
BstF5I GGATG 6 cut(s) 106, 1086, 1318, 1501, 1512, 2159
BstFNI CGCG 1 cut(s) 1374
BstH2I RGCGCY 1 cut(s) 1875
BstHHI GCGC 3 cut(s) 1318, 1874, 2074
BstKTI GATC 8 cut(s) 208, 499, 1378, 1512, 1516, 1687, 1714, 1816
BstMAI GTCTC 2 cut(s) 738, 1457
BstMBI GATC 8 cut(s) 205, 496, 1375, 1509, 1513, 1684, 1711, 1813
BstNI CCWGG 2 cut(s) 111, 2055
BstNSI RCATGY 5 cut(s) 159, 627, 797, 881, 999
BstSCI CCNGG 2 cut(s) 109, 2053
BstSFI CTRYAG 3 cut(s) 91, 1968, 2064
BstSLI GKGCMC 4 cut(s) 1631, 1648, 1741, 1861
BstUI CGCG 1 cut(s) 1374
BstV1I GCAGC 4 cut(s) 453, 719, 1125, 2095
BstV2I GAAGAC 3 cut(s) 176, 863, 2003
BstX2I RGATCY 4 cut(s) 205, 496, 1684, 1711
BstYI RGATCY 4 cut(s) 205, 496, 1684, 1711
Bsu15I ATCGAT 2 cut(s) 1512, 2122
Bsu36I CCTNAGG 1 cut(s) 803
BsuI GTATCC 1 cut(s) 1605
BsuRI GGCC 6 cut(s) 115, 576, 1077, 2053, 2222, 2275
BsuTUI ATCGAT 2 cut(s) 1512, 2122
BtgI CCRYGG 1 cut(s) 1988
BtrI CACGTC 1 cut(s) 1439
BtsCI GGATG 6 cut(s) 106, 1086, 1318, 1501, 1512, 2159
BtsI GCAGTG 7 cut(s) 100, 577, 650, 872, 1178, 1903, 1977
BveI ACCTGC 1 cut(s) 1208
Cac8I GCNNGC 6 cut(s) 179, 879, 989, 1173, 1533, 2076
CfoI GCGC 3 cut(s) 1318, 1874, 2074
Cfr10I RCCGGY 1 cut(s) 435
Cfr13I GGNCC 4 cut(s) 113, 799, 1360, 2207
ClaI ATCGAT 2 cut(s) 1512, 2122
CseI GACGC 1 cut(s) 1380
Csp6I GTAC 4 cut(s) 663, 999, 1414, 2132
CspAI ACCGGT 1 cut(s) 435
CspCI CAANNNNNGTGG 4 cut(s) 285, 320, 832, 867
CviQI GTAC 4 cut(s) 663, 999, 1414, 2132
DdeI CTNAG 7 cut(s) 71, 702, 803, 1254, 1347, 1367, 2113
DpnI GATC 8 cut(s) 207, 498, 1377, 1511, 1515, 1686, 1713, 1815
DpnII GATC 8 cut(s) 205, 496, 1375, 1509, 1513, 1684, 1711, 1813
DraI TTTAAA 1 cut(s) 364
EaeI YGGCCR 2 cut(s) 574, 1075
Eam1104I CTCTTC 2 cut(s) 1484, 2040
EarI CTCTTC 2 cut(s) 1484, 2040
EciI GGCGGA 1 cut(s) 427
Eco130I CCWWGG 3 cut(s) 974, 1988, 2223
Eco24I GRGCYC 2 cut(s) 1560, 2159
Eco31I GGTCTC 1 cut(s) 1457
Eco47I GGWCC 3 cut(s) 799, 1360, 2207
Eco57I CTGAAG 2 cut(s) 1502, 2322
Eco81I CCTNAGG 1 cut(s) 803
Eco88I CYCGRG 1 cut(s) 1821
EcoRI GAATTC 1 cut(s) 2215
EcoRII CCWGG 2 cut(s) 109, 2053
EcoT14I CCWWGG 3 cut(s) 974, 1988, 2223
EcoT22I ATGCAT 2 cut(s) 879, 1798
EcoT38I GRGCYC 2 cut(s) 1560, 2159
ErhI CCWWGG 3 cut(s) 974, 1988, 2223
FalI AAGNNNNNCTT 4 cut(s) 161, 193, 1081, 1113
FaqI GGGAC 4 cut(s) 226, 683, 842, 1957
FauI CCCGC 1 cut(s) 1612
FblI GTMKAC 1 cut(s) 1435
Fnu4HI GCNGC 8 cut(s) 442, 733, 1114, 1545, 1827, 1911, 2109, 2273
FokI GGATG 6 cut(s) 93, 1093, 1325, 1508, 1519, 2146
FriOI GRGCYC 2 cut(s) 1560, 2159
Fsp4HI GCNGC 8 cut(s) 442, 733, 1114, 1545, 1827, 1911, 2109, 2273
FspBI CTAG 5 cut(s) 975, 1122, 1719, 1950, 2190
GlaI GCGC 3 cut(s) 1317, 1873, 2073
GluI GCNGC 8 cut(s) 442, 733, 1114, 1545, 1827, 1911, 2109, 2273
GsaI CCCAGC 1 cut(s) 1654
HaeII RGCGCY 1 cut(s) 1875
HaeIII GGCC 6 cut(s) 115, 576, 1077, 2053, 2222, 2275
HapII CCGG 5 cut(s) 436, 1078, 1107, 1658, 2016
HgaI GACGC 1 cut(s) 1380
HhaI GCGC 3 cut(s) 1318, 1874, 2074
Hin6I GCGC 3 cut(s) 1316, 1872, 2072
HinP1I GCGC 3 cut(s) 1316, 1872, 2072
HindIII AAGCTT 4 cut(s) 175, 1149, 1229, 1529
HinfI GANTC 7 cut(s) 211, 557, 1103, 1193, 2012, 2030, 2186
HpaII CCGG 5 cut(s) 436, 1078, 1107, 1658, 2016
HphI GGTGA 5 cut(s) 31, 890, 1004, 1340, 2153
Hpy166II GTNNAC 9 cut(s) 752, 956, 1436, 1646, 1678, 1739, 1859, 1885, 2132
Hpy188I TCNGA 7 cut(s) 210, 1220, 1348, 1360, 1506, 1611, 1689
Hpy188III TCNNGA 5 cut(s) 269, 805, 1031, 1517, 2190
Hpy8I GTNNAC 9 cut(s) 752, 956, 1436, 1646, 1678, 1739, 1859, 1885, 2132
Hpy99I CGWCG 1 cut(s) 195
HpyAV CCTTC 6 cut(s) 423, 1373, 1477, 1949, 2261, 2317
HpyCH4III ACNGT 8 cut(s) 7, 164, 572, 749, 1639, 2002, 2141, 2199
HpyCH4IV ACGT 2 cut(s) 1438, 2082
HpyF3I CTNAG 7 cut(s) 71, 702, 803, 1254, 1347, 1367, 2113
HpySE526I ACGT 2 cut(s) 1438, 2082
HspAI GCGC 3 cut(s) 1316, 1872, 2072
KpnI GGTACC 1 cut(s) 666
Kzo9I GATC 8 cut(s) 205, 496, 1375, 1509, 1513, 1684, 1711, 1813
LmnI GCTCC 6 cut(s) 690, 976, 1261, 1323, 1555, 1961
Lsp1109I GCAGC 4 cut(s) 453, 719, 1125, 2095
LweI GCATC 9 cut(s) 246, 1273, 1303, 1397, 1467, 1714, 1910, 1922, 2059
MaeI CTAG 5 cut(s) 975, 1122, 1719, 1950, 2190
MaeII ACGT 2 cut(s) 1438, 2082
MaeIII GTNAC 9 cut(s) 313, 566, 839, 896, 1010, 1054, 1582, 2002, 2141
MalI GATC 8 cut(s) 207, 498, 1377, 1511, 1515, 1686, 1713, 1815
MbiI CCGCTC 3 cut(s) 1264, 1607, 1826
MboI GATC 8 cut(s) 205, 496, 1375, 1509, 1513, 1684, 1711, 1813
MfeI CAATTG 2 cut(s) 1188, 2103
MflI RGATCY 4 cut(s) 205, 496, 1684, 1711
MhlI GDGCHC 8 cut(s) 973, 1425, 1560, 1631, 1648, 1741, 1861, 2159
MlsI TGGCCA 1 cut(s) 576
MluNI TGGCCA 1 cut(s) 576
MlyI GAGTC 1 cut(s) 2021
MmeI TCCRAC 4 cut(s) 239, 1827, 1914, 2000
Mox20I TGGCCA 1 cut(s) 576
Mph1103I ATGCAT 2 cut(s) 879, 1798
MscI TGGCCA 1 cut(s) 576
MseI TTAA 7 cut(s) 9, 134, 144, 150, 363, 533, 1623
MslI CAYNNNNRTG 5 cut(s) 552, 655, 882, 1017, 1326
Msp20I TGGCCA 1 cut(s) 576
MspA1I CMGCKG 2 cut(s) 732, 1547
MspI CCGG 5 cut(s) 436, 1078, 1107, 1658, 2016
MspR9I CCNGG 2 cut(s) 111, 2055
MunI CAATTG 2 cut(s) 1188, 2103
Mva1269I GAATGC 3 cut(s) 362, 547, 1798
MvaI CCWGG 2 cut(s) 111, 2055
MvnI CGCG 1 cut(s) 1374
NcoI CCATGG 1 cut(s) 1988
NdeII GATC 8 cut(s) 205, 496, 1375, 1509, 1513, 1684, 1711, 1813
NlaIV GGNNCC 8 cut(s) 207, 434, 498, 664, 765, 1557, 1686, 2208
NmeAIII GCCGAG 1 cut(s) 1404
NmuCI GTSAC 6 cut(s) 313, 566, 896, 1010, 1054, 2141
NsiI ATGCAT 2 cut(s) 879, 1798
NspI RCATGY 5 cut(s) 159, 627, 797, 881, 999
PaeI GCATGC 1 cut(s) 881
PaeR7I CTCGAG 1 cut(s) 1821
PciI ACATGT 1 cut(s) 995
PctI GAATGC 3 cut(s) 362, 547, 1798
PfeI GAWTC 6 cut(s) 211, 557, 1103, 1193, 2030, 2186
PinAI ACCGGT 1 cut(s) 435
PkrI GCNGC 8 cut(s) 443, 734, 1115, 1546, 1828, 1912, 2110, 2274
PleI GAGTC 1 cut(s) 2020
PpsI GAGTC 1 cut(s) 2020
PscI ACATGT 1 cut(s) 995
Psp6I CCWGG 2 cut(s) 109, 2053
PspFI CCCAGC 1 cut(s) 1650
PspGI CCWGG 2 cut(s) 109, 2053
PspN4I GGNNCC 8 cut(s) 207, 434, 498, 664, 765, 1557, 1686, 2208
PspPI GGNCC 4 cut(s) 113, 799, 1360, 2207
PspXI VCTCGAGB 1 cut(s) 1821
PstI CTGCAG 2 cut(s) 95, 1972
PsuI RGATCY 4 cut(s) 205, 496, 1684, 1711
PvuII CAGCTG 1 cut(s) 732
RsaI GTAC 4 cut(s) 664, 1000, 1415, 2133
RsaNI GTAC 4 cut(s) 663, 999, 1414, 2132
RseI CAYNNNNRTG 5 cut(s) 552, 655, 882, 1017, 1326
SaqAI TTAA 7 cut(s) 9, 134, 144, 150, 363, 533, 1623
SatI GCNGC 8 cut(s) 442, 733, 1114, 1545, 1827, 1911, 2109, 2273
Sau3AI GATC 8 cut(s) 205, 496, 1375, 1509, 1513, 1684, 1711, 1813
Sau96I GGNCC 4 cut(s) 113, 799, 1360, 2207
ScaI AGTACT 1 cut(s) 1415
SchI GAGTC 1 cut(s) 2021
ScrFI CCNGG 2 cut(s) 111, 2055
SduI GDGCHC 8 cut(s) 973, 1425, 1560, 1631, 1648, 1741, 1861, 2159
SfaNI GCATC 9 cut(s) 246, 1273, 1303, 1397, 1467, 1714, 1910, 1922, 2059
SfcI CTRYAG 3 cut(s) 91, 1968, 2064
Sfr274I CTCGAG 1 cut(s) 1821
SgrAI CRCCGGYG 1 cut(s) 435
SinI GGWCC 3 cut(s) 799, 1360, 2207
SlaI CTCGAG 1 cut(s) 1821
SmiMI CAYNNNNRTG 5 cut(s) 552, 655, 882, 1017, 1326
SmlI CTYRAG 2 cut(s) 1454, 1821
SmoI CTYRAG 2 cut(s) 1454, 1821
SphI GCATGC 1 cut(s) 881
SspMI CTAG 5 cut(s) 975, 1122, 1719, 1950, 2190
StyD4I CCNGG 2 cut(s) 109, 2053
StyI CCWWGG 3 cut(s) 974, 1988, 2223
TaaI ACNGT 8 cut(s) 7, 164, 572, 749, 1639, 2002, 2141, 2199
TaiI ACGT 2 cut(s) 1441, 2085
TaqI TCGA 6 cut(s) 824, 1512, 1822, 2122, 2246, 2252
TaqII GACCGA 1 cut(s) 336
TatI WGTACW 3 cut(s) 998, 1413, 2131
TauI GCSGC 4 cut(s) 1547, 1829, 1913, 2275
TfiI GAWTC 6 cut(s) 211, 557, 1103, 1193, 2030, 2186
Tru1I TTAA 7 cut(s) 9, 134, 144, 150, 363, 533, 1623
Tru9I TTAA 7 cut(s) 9, 134, 144, 150, 363, 533, 1623
TseFI GTSAC 6 cut(s) 313, 566, 896, 1010, 1054, 2141
TseI GCWGC 4 cut(s) 441, 732, 1113, 2108
Tsp45I GTSAC 6 cut(s) 313, 566, 896, 1010, 1054, 2141
TspDTI ATGAA 9 cut(s) 33, 1161, 1260, 1451, 1568, 1680, 1692, 2022, 2157
TspGWI ACGGA 2 cut(s) 208, 623
VneI GTGCAC 3 cut(s) 1644, 1737, 1857
VpaK11BI GGWCC 3 cut(s) 799, 1360, 2207
XapI RAATTY 3 cut(s) 386, 1306, 2215
XbaI TCTAGA 1 cut(s) 2189
XceI RCATGY 5 cut(s) 159, 627, 797, 881, 999
XcmI CCANNNNNNNNNTGG 1 cut(s) 2082
XhoI CTCGAG 1 cut(s) 1821
XmaJI CCTAGG 1 cut(s) 974
XmiI GTMKAC 1 cut(s) 1435
XspI CTAG 5 cut(s) 975, 1122, 1719, 1950, 2190
ZrmI AGTACT 1 cut(s) 1415
Zsp2I ATGCAT 2 cut(s) 879, 1798
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.