Rroxscaffold_3G00275290

NF-X1-type zinc finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0011120
Physical Location & Seq
Forward (+)
66769932 .. 66774457
4526 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00275290.1

Sequence Viewer

Length: 2811 bp
ATGGCTTCTCAGGTCCGAAGTGAGCGTAGGGAGAATAATAGGTCCAGGAATCCTGCTCAGCCTCGCTGGGTGCCCAGAGGATCCAACCCTACCGCCGCTGCCGTGAACCCGCCGAGCTTTGGTTCGAACCTACCCGATGGGAATGCTGGGATGCCCAACAACAGCTCTGAAGCAGGAGAGGGCCGGCAGCATAGAGGAGGACATGGTGGAAATGGAGGGCAGCAGAACCACATTTCGGCACCAGCAGAGAGTCGGCAGCATAGAGGAAATGGTGGGCAACTCAATTATCTGTCAGCGGCACCGTCAGAGAGTCGGCAGCATAGAGGGGGACATAATGGAAATGGAGGCCAGTCCAACTACAGCTTCACAGCACCGGATGGTCGGCAGCAGAGAGGAGGACATAATGCAAGTGTGGGGCAGTTCAGTCACAGTTCTGCGACATCAGAGAGTCGGCTGCATAGAGGAGCACACAATGGGAATGGAGGGCAGTCCAGTCACAGCTTTACATTATCGGAGAATTGGCAGCACAGAGGAGGACATAATGGTTCCAGGGGTAATGTGGTTCGGCCAATGAATCATAGAAGGGAGAGGGAAAGGAGTGAGAGTCAGGAGGGGATGGGCATGAAGGACTCTAGTTTGCCGCAGCTTGTGCAAGAAATTCAGGAAAAACTGACTAAGGGCACTGTTGAGTGCATGATTTGTTATGATATGGTGAGGAGGTCTGCACCTGTATGGTCCTGCTCAAGCTGTTTCTCGATTTTCCATCTGAATTGCATCAAGAAATGGGCTCGAGCTCCCACTTCTATTGATATGTCTGCAGGGAAGAATCAGGGATTTAATTGGCGGTGTCCAGGATGTCAGTCTGTACAGCTCACATCGTCAAAGGAGATTCGCTACATGTGTTTTTGTGGTAAGCGGACAGACCCACCTTCGGATTTGTATTTGACACCACATTCATGTGGAGAACATTGTGGGAAGCCACTCGAGAAGGAGGTCCCAGGAAAGGGAATGAGTAAGGATGATCTTTGCCCTCATATGTGTGTCTTGCAGTGCCACCCAGGTCCATGCCCTCCTTGTAAAGCATTCGCACCGCCACGGTTATGCCCTTGTGGAAAGAAAACAATAACTACAAGATGCTCAGATCGGACTTCTGTTCTGACTTGTGGCAATCTTTGTAGTAAGCTTCTTGATTGTGGGCGTCACCGCTGTGAGCGGAAGTGCCATGTGGGTCCTTGTGATCCTTGTCAGGTTCCATTCAATGCTTCTTGCTTTTGCCTGAAAAAGCTGGAGGCCGTTCTTTGTGGAGAGATGATAGTAAAGGGAGAGGTGAAAGCAGAAGATGGTGTTTTCTCTTGCAGTTCTAGTTGTGGAAAGAAGCTTAGCTGTGGCAATCATGTTTGCAGTGAAATTTGTCATCCAGGCCCTTGTGGAGAGTGCATTTTAATGCCACGGAATATCAAGACATGCCACTGTGGGAAAACAAGCTTGCAGGAGGAACGCCAGAGTTGTTTGGATCCGATTCCGACTTGTTCACAAATATGTGACAAGACTCTCCCTTGTGGGGTGCACCAGTGTCAACAGGTGTGCCATACTGGTGATTGCCCCCCTTGTTTGGTTAAAGTAACCCAAAAATGTCGCTGTGAATCAACTTCTCGGAATGTGGAGTGCTGTAAAACAACAATGGAGGATGAGAAATTTACTTGTGATAAGCCTTGTGGACGGAAGAAGAATTGTGGAAGGCACCGCTGCAGTGAAAGGTGCTGTCCTCTTTCTAATTCAAATAATCATCTTTCAGGGGATTGGGATCCTCACTTGTGCTCCATGCCCTGTGGGAAGAAATTAAGGTGCGGGCAGCACTCTTGTGAATCGCTGTGTCACAGTGGCCACTGTCCTCCCTGCCTGGATACAATCTTTACTGACTTGACATGTGCATGTGGGAGAACTTCAATCCCTCCTCCATTGCCCTGTGGTACACCTCCTCCATCATGTCAGCTCCCTTGTTCGGTGCCTCAGCCTTGTGGACATTCATCTTCTCACAGCTGCCACTTTGGAGACTGCCCACCTTGTTCAGTGCCTGTAGCAAAGGAGTGCATCGGTGGACATGTGGTCCTCAGGAACATTCCTTGTGGATCGAGGGACATTAAATGTAACAAGCTCTGTGGGAAGATGAGGCAATGCGGTATGCATGCTTGCGGCAGAACATGTCACCCACCGCCTTGTGAGTGTTCCTCGTCAGCAGAAGCCGGCTCAAAATCTTCTTGTGGACAGAAATGTGGAGCTCCTAGGAGAGATTGCAGGCATACATGTACTGCACCTTGTCACCCTTATGCTTCCTGTCCAGATGCGAGATGTGATTTCCTTGTCACAATTACTTGTTCTTGTGGCCGGATAACAGCAAATGTTCCTTGCGATTCTGGTGGCACCTCTGCTAGTTTTAATGCTGATACTGTGTTTGAAGCTTCCATTATCCAAAAGTTGCCAGTACCGCTTCAACCAGTTGAAGCCATCAACAAGAAGATCCCACTTGGACAGAGGAAGCTCATGTGTGATGATGAATGTGCTAAGTTGGAGCGGAAACGTGTTCTTGCAGATGCTTTTGATATAGTTCCTCCAAATTTGGATGCACTCCATTTTGGTGAGACTACTGTTATTTCTGAGTTGCTGTCGGACCTTTTCAGGCGTGATCCAAAATGGGTATTATCGGTGGAGGAGAGATGCAAACAATTGGTTCTTGGGAAGAGCAAAGGTGCTACAAGTGGCCTCCGGGTCCATGTTTTTTGTCCAATGCTGAAGGAAAAGAGATGTGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000122 GO:0000976 GO:0000977 GO:0000981 GO:0000982 GO:0001012 GO:0001067 GO:0001078 GO:0001227 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006082 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006970 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009416 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009636 GO:0009642 GO:0009651 GO:0009696 GO:0009697 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010188 GO:0010310 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0010817 GO:0016053 GO:0016070 GO:0016999 GO:0017000 GO:0017144 GO:0018130 GO:0018958 GO:0019219 GO:0019222 GO:0019438 GO:0019752 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032774 GO:0032787 GO:0034641 GO:0034645 GO:0034654 GO:0042221 GO:0042445 GO:0042446 GO:0042537 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0046189 GO:0046394 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051193 GO:0051252 GO:0051253 GO:0051704 GO:0051707 GO:0060255 GO:0065007 GO:0065008 GO:0071704 GO:0072330 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:0098542 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901615 GO:1901617 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:2000112 GO:2000113 GO:2000377 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko05165 map05165
Pfam Domains
Protein Families
K_trans R3H zf-NF-X1

Protein Analysis

936

Amino Acids

101.45

Weight (kDa)

8.67

Isoelectric Point (pI)

57.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-NF-X1 PF01422 398 - 416 4.9e-06 NF-X1 type zinc finger
zf-NF-X1 PF01422 520 - 537 2.2e-06 NF-X1 type zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011120)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10170 AT1G10170 AT1G10170 AT1G10170
fragaria_vesca FvH4_5g17660
malus_domestica MD06G1232100.v1.1 MD14G1238900.v1.1
prunus_persica Prupe.5G236400_v2.0.a1
pyrus_communis pycom06g20620 pycom14g19990
rosa_chinensis RchiOBHm_Chr7g0178011
rosa_laevigata RLG00000005482
rosa_multiflora Rmu_sc0013399.1_g000001
rosa_roxburghii Rroxscaffold_3G00275290
rosa_rugosa Rorug06G0412300
rosa_samantha Rh7AG011800 Rh7BG011200 Rh7CG011800 Rh7DG011800
rosa_wichuraiana Rw7G000930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 6 cut(s) 70, 238, 298, 1740, 2005, 2420
AccBSI CCGCTC 2 cut(s) 1213, 2572
AcoI YGGCCR 3 cut(s) 566, 1882, 2383
AcsI RAATTY 4 cut(s) 657, 1407, 1694, 2614
AcuI CTGAAG 2 cut(s) 189, 2810
AcyI GRCGYC 1 cut(s) 1198
AfaI GTAC 4 cut(s) 867, 1972, 2308, 2484
AfiI CCNNNNNNNGG 8 cut(s) 119, 235, 931, 1003, 1004, 1561, 1612, 2002
AflIII ACRYGT 6 cut(s) 897, 1925, 2101, 2201, 2303, 2578
AgsI TTSAA 6 cut(s) 1258, 1779, 1947, 2456, 2492, 2501
AhdI GACNNNNNGTC 1 cut(s) 2105
AjnI CCWGG 7 cut(s) 44, 548, 850, 997, 1057, 1417, 1899
AjuI GAANNNNNNNTTGG 2 cut(s) 347, 379
AleI CACNNNNGTG 2 cut(s) 1206, 1860
Alw21I GWGCWC 5 cut(s) 469, 796, 1569, 1820, 2281
Alw26I GTCTC 2 cut(s) 2046, 2633
Alw44I GTGCAC 1 cut(s) 1565
AlwNI CAGNNNCTG 1 cut(s) 2075
Ama87I CYCGRG 2 cut(s) 789, 983
AoxI GGCC 8 cut(s) 181, 346, 566, 1290, 1420, 1882, 2383, 2758
ApaLI GTGCAC 1 cut(s) 1565
ApoI RAATTY 4 cut(s) 657, 1407, 1694, 2614
AspA2I CCTAGG 1 cut(s) 2282
AsuC2I CCSGG 1 cut(s) 2765
AsuHPI GGTGA 7 cut(s) 724, 1193, 1339, 1607, 2198, 2312, 2648
AsuII TTCGAA 1 cut(s) 125
AvaI CYCGRG 2 cut(s) 789, 983
AvaII GGWCC 9 cut(s) 13, 42, 735, 994, 1061, 1229, 2107, 2668, 2767
AvrII CCTAGG 1 cut(s) 2282
AxyI CCTNAGG 1 cut(s) 2111
BaeGI GKGCMC 3 cut(s) 75, 683, 1569
BalI TGGCCA 1 cut(s) 1884
BamHI GGATCC 3 cut(s) 80, 1513, 1804
BanI GGYRCC 6 cut(s) 70, 238, 298, 1740, 2005, 2420
BanII GRGCYC 3 cut(s) 790, 796, 2281
Bbv12I GWGCWC 5 cut(s) 469, 796, 1569, 1820, 2281
BbvCI CCTCAGC 1 cut(s) 2010
BccI CCATC 7 cut(s) 131, 371, 610, 771, 1334, 1990, 2513
BceAI ACGGC 2 cut(s) 86, 1277
BcgI CGANNNNNNTGC 2 cut(s) 125, 159
BciT130I CCWGG 7 cut(s) 46, 550, 852, 999, 1059, 1419, 1901
BciVI GTATCC 1 cut(s) 1897
BcnI CCSGG 1 cut(s) 2765
BcoDI GTCTC 2 cut(s) 2046, 2633
BfaI CTAG 4 cut(s) 633, 1362, 2283, 2430
BfmI CTRYAG 4 cut(s) 358, 816, 1747, 2076
BfuI GTATCC 1 cut(s) 1897
BlnI CCTAGG 1 cut(s) 2282
BlpI GCTNAGC 2 cut(s) 57, 1379
Bme1390I CCNGG 8 cut(s) 46, 550, 852, 999, 1059, 1419, 1901, 2765
Bme18I GGWCC 9 cut(s) 13, 42, 735, 994, 1061, 1229, 2107, 2668, 2767
BmeRI GACNNNNNGTC 1 cut(s) 2105
BmeT110I CYCGRG 2 cut(s) 789, 983
BmrFI CCNGG 8 cut(s) 46, 550, 852, 999, 1059, 1419, 1901, 2765
BmsI GCATC 8 cut(s) 141, 783, 1124, 2100, 2332, 2581, 2611, 2705
BplI GAGNNNNNCTC 2 cut(s) 2213, 2245
BpmI CTGGAG 1 cut(s) 1307
Bpu10I CCTNAGC 1 cut(s) 2010
Bpu1102I GCTNAGC 2 cut(s) 57, 1379
Bpu14I TTCGAA 1 cut(s) 125
BpuEI CTTGAG 1 cut(s) 727
BpuMI CCSGG 1 cut(s) 2765
BsaBI GATNNNNATC 1 cut(s) 1803
BsaHI GRCGYC 1 cut(s) 1198
BsaJI CCNNGG 6 cut(s) 549, 997, 1057, 1094, 1448, 2282
BsaWI WCCGGW 1 cut(s) 373
BsaXI ACNNNNNCTCC 6 cut(s) 589, 619, 1802, 1832, 1963, 1993
Bsc4I CCNNNNNNNGG 8 cut(s) 119, 235, 931, 1003, 1004, 1561, 1612, 2002
Bse118I RCCGGY 2 cut(s) 183, 2243
Bse1I ACTGG 6 cut(s) 349, 492, 1570, 1597, 2480, 2495
Bse21I CCTNAGG 1 cut(s) 2111
Bse3DI GCAATG 2 cut(s) 1958, 2180
Bse8I GATNNNNATC 1 cut(s) 1803
BseBI CCWGG 7 cut(s) 46, 550, 852, 999, 1059, 1419, 1901
BseDI CCNNGG 6 cut(s) 549, 997, 1057, 1094, 1448, 2282
BseGI GGATG 8 cut(s) 156, 382, 621, 860, 1024, 1414, 1693, 2626
BseJI GATNNNNATC 1 cut(s) 1803
BseLI CCNNNNNNNGG 8 cut(s) 119, 235, 931, 1003, 1004, 1561, 1612, 2002
BseMI GCAATG 2 cut(s) 1958, 2180
BseMII CTCAG 6 cut(s) 23, 71, 1152, 2024, 2125, 2646
BseNI ACTGG 6 cut(s) 349, 492, 1570, 1597, 2480, 2495
BseRI GAGGAG 8 cut(s) 210, 408, 477, 546, 730, 1944, 1968, 2723
BseSI GKGCMC 3 cut(s) 75, 683, 1569
BseYI CCCAGC 2 cut(s) 66, 146
BsgI GTGCAG 2 cut(s) 708, 2295
BshFI GGCC 8 cut(s) 183, 348, 568, 1292, 1422, 1884, 2385, 2760
BshNI GGYRCC 6 cut(s) 70, 238, 298, 1740, 2005, 2420
BsiHKAI GWGCWC 5 cut(s) 469, 796, 1569, 1820, 2281
BsiHKCI CYCGRG 2 cut(s) 789, 983
BsiSI CCGG 5 cut(s) 184, 374, 2244, 2386, 2764
BslFI GGGAC 3 cut(s) 342, 980, 2150
BslI CCNNNNNNNGG 8 cut(s) 119, 235, 931, 1003, 1004, 1561, 1612, 2002
BsmAI GTCTC 2 cut(s) 2046, 2633
BsmFI GGGAC 3 cut(s) 342, 980, 2150
BsmI GAATGC 2 cut(s) 148, 1082
BsnI GGCC 8 cut(s) 183, 348, 568, 1292, 1422, 1884, 2385, 2760
BsoBI CYCGRG 2 cut(s) 789, 983
Bsp119I TTCGAA 1 cut(s) 125
Bsp1286I GDGCHC 8 cut(s) 75, 469, 683, 790, 796, 1569, 1820, 2281
Bsp1407I TGTACA 1 cut(s) 865
Bsp143I GATC 9 cut(s) 80, 1021, 1141, 1237, 1513, 1804, 2129, 2517, 2683
Bsp1720I GCTNAGC 2 cut(s) 57, 1379
BspANI GGCC 8 cut(s) 183, 348, 568, 1292, 1422, 1884, 2385, 2760
BspCNI CTCAG 6 cut(s) 22, 70, 1151, 2023, 2124, 2647
BspMAI CTGCAG 2 cut(s) 820, 1751
BspQI GCTCTTC 1 cut(s) 2732
BspT104I TTCGAA 1 cut(s) 125
BspT107I GGYRCC 6 cut(s) 70, 238, 298, 1740, 2005, 2420
BsrBI CCGCTC 2 cut(s) 1213, 2572
BsrDI GCAATG 2 cut(s) 1958, 2180
BsrFI RCCGGY 2 cut(s) 183, 2243
BsrGI TGTACA 1 cut(s) 865
BsrI ACTGG 6 cut(s) 349, 492, 1570, 1597, 2480, 2495
BssAI RCCGGY 2 cut(s) 183, 2243
BssECI CCNNGG 6 cut(s) 549, 997, 1057, 1094, 1448, 2282
BssMI GATC 9 cut(s) 80, 1021, 1141, 1237, 1513, 1804, 2129, 2517, 2683
BssNI GRCGYC 1 cut(s) 1198
BssT1I CCWWGG 1 cut(s) 2282
Bst2UI CCWGG 7 cut(s) 46, 550, 852, 999, 1059, 1419, 1901
Bst4CI ACNGT 9 cut(s) 303, 431, 685, 1098, 1472, 1880, 1889, 2449, 2647
Bst6I CTCTTC 1 cut(s) 2732
BstACI GRCGYC 1 cut(s) 1198
BstAPI GCANNNNNTGC 1 cut(s) 649
BstAUI TGTACA 1 cut(s) 865
BstBI TTCGAA 1 cut(s) 125
BstC8I GCNNGC 7 cut(s) 185, 1487, 1850, 2187, 2191, 2245, 2297
BstDEI CTNAG 9 cut(s) 9, 57, 675, 1138, 1379, 2010, 2111, 2562, 2655
BstDSI CCRYGG 2 cut(s) 1094, 1448
BstF5I GGATG 8 cut(s) 156, 382, 621, 860, 1024, 1414, 1693, 2626
BstKTI GATC 9 cut(s) 83, 1024, 1144, 1240, 1516, 1807, 2132, 2520, 2686
BstMAI GTCTC 2 cut(s) 2046, 2633
BstMBI GATC 9 cut(s) 80, 1021, 1141, 1237, 1513, 1804, 2129, 2517, 2683
BstMWI GCNNNNNNNGC 2 cut(s) 649, 2485
BstNI CCWGG 7 cut(s) 46, 550, 852, 999, 1059, 1419, 1901
BstNSI RCATGY 8 cut(s) 901, 1467, 1929, 1935, 2105, 2189, 2205, 2307
BstSCI CCNGG 8 cut(s) 44, 548, 850, 997, 1057, 1417, 1899, 2763
BstSFI CTRYAG 4 cut(s) 358, 816, 1747, 2076
BstSLI GKGCMC 3 cut(s) 75, 683, 1569
BstX2I RGATCY 4 cut(s) 80, 1513, 1804, 2517
BstYI RGATCY 4 cut(s) 80, 1513, 1804, 2517
Bsu36I CCTNAGG 1 cut(s) 2111
BsuI GTATCC 1 cut(s) 1897
BsuRI GGCC 8 cut(s) 183, 348, 568, 1292, 1422, 1884, 2385, 2760
BtgI CCRYGG 2 cut(s) 1094, 1448
BtsCI GGATG 8 cut(s) 156, 382, 621, 860, 1024, 1414, 1693, 2626
BtsI GCAGTG 3 cut(s) 1055, 1408, 1756
BtsIMutI CAGTG 9 cut(s) 681, 1055, 1408, 1468, 1577, 1756, 1885, 1885, 2076
Cac8I GCNNGC 7 cut(s) 185, 1487, 1850, 2187, 2191, 2245, 2297
CaiI CAGNNNCTG 1 cut(s) 2075
Cfr10I RCCGGY 2 cut(s) 183, 2243
CseI GACGC 1 cut(s) 1187
Csp6I GTAC 4 cut(s) 866, 1971, 2307, 2483
CspCI CAANNNNNGTGG 4 cut(s) 787, 822, 2140, 2175
CviQI GTAC 4 cut(s) 866, 1971, 2307, 2483
DdeI CTNAG 9 cut(s) 9, 57, 675, 1138, 1379, 2010, 2111, 2562, 2655
DpnI GATC 9 cut(s) 82, 1023, 1143, 1239, 1515, 1806, 2131, 2519, 2685
DpnII GATC 9 cut(s) 80, 1021, 1141, 1237, 1513, 1804, 2129, 2517, 2683
DriI GACNNNNNGTC 1 cut(s) 2105
EaeI YGGCCR 3 cut(s) 566, 1882, 2383
Eam1104I CTCTTC 1 cut(s) 2732
Eam1105I GACNNNNNGTC 1 cut(s) 2105
EarI CTCTTC 1 cut(s) 2732
Ecl136II GAGCTC 2 cut(s) 794, 2279
Eco130I CCWWGG 1 cut(s) 2282
Eco24I GRGCYC 3 cut(s) 790, 796, 2281
Eco47I GGWCC 9 cut(s) 13, 42, 735, 994, 1061, 1229, 2107, 2668, 2767
Eco53kI GAGCTC 2 cut(s) 794, 2279
Eco57I CTGAAG 2 cut(s) 189, 2810
Eco81I CCTNAGG 1 cut(s) 2111
Eco88I CYCGRG 2 cut(s) 789, 983
EcoICRI GAGCTC 2 cut(s) 794, 2279
EcoO109I RGGNCCY 3 cut(s) 994, 1229, 1421
EcoRII CCWGG 7 cut(s) 44, 548, 850, 997, 1057, 1417, 1899
EcoT14I CCWWGG 1 cut(s) 2282
EcoT22I ATGCAT 1 cut(s) 2187
EcoT38I GRGCYC 3 cut(s) 790, 796, 2281
ErhI CCWWGG 1 cut(s) 2282
FaqI GGGAC 3 cut(s) 342, 980, 2150
FauI CCCGC 2 cut(s) 117, 1841
FauNDI CATATG 1 cut(s) 1035
FokI GGATG 8 cut(s) 163, 389, 628, 867, 1031, 1401, 1700, 2633
FriOI GRGCYC 3 cut(s) 790, 796, 2281
FspBI CTAG 4 cut(s) 633, 1362, 2283, 2430
GsaI CCCAGC 2 cut(s) 70, 150
GsuI CTGGAG 1 cut(s) 1307
HaeIII GGCC 8 cut(s) 183, 348, 568, 1292, 1422, 1884, 2385, 2760
HapII CCGG 5 cut(s) 184, 374, 2244, 2386, 2764
HgaI GACGC 1 cut(s) 1187
Hin1I GRCGYC 1 cut(s) 1198
HincII GTYRAC 1 cut(s) 1577
HindII GTYRAC 1 cut(s) 1577
HindIII AAGCTT 4 cut(s) 1181, 1376, 1483, 2457
HpaII CCGG 5 cut(s) 184, 374, 2244, 2386, 2764
HphI GGTGA 7 cut(s) 724, 1193, 1339, 1607, 2198, 2312, 2648
Hpy166II GTNNAC 9 cut(s) 106, 1532, 1567, 1577, 1718, 1973, 2021, 2099, 2264
Hpy188III TCNNGA 9 cut(s) 608, 662, 754, 778, 985, 1187, 1459, 2113, 2339
Hpy8I GTNNAC 9 cut(s) 106, 1532, 1567, 1577, 1718, 1973, 2021, 2099, 2264
HpyAV CCTTC 6 cut(s) 576, 619, 939, 982, 1731, 2785
HpyCH4III ACNGT 9 cut(s) 303, 431, 685, 1098, 1472, 1880, 1889, 2449, 2647
HpyCH4IV ACGT 1 cut(s) 2578
HpyF10VI GCNNNNNNNGC 2 cut(s) 649, 2485
HpyF3I CTNAG 9 cut(s) 9, 57, 675, 1138, 1379, 2010, 2111, 2562, 2655
HpySE526I ACGT 1 cut(s) 2578
Hsp92I GRCGYC 1 cut(s) 1198
KroI GCCGGC 2 cut(s) 183, 2243
KroNI GCCGGC 2 cut(s) 185, 2245
Kzo9I GATC 9 cut(s) 80, 1021, 1141, 1237, 1513, 1804, 2129, 2517, 2683
LguI GCTCTTC 1 cut(s) 2732
LmnI GCTCC 7 cut(s) 464, 799, 1823, 1998, 2276, 2284, 2569
LweI GCATC 8 cut(s) 141, 783, 1124, 2100, 2332, 2581, 2611, 2705
MaeI CTAG 4 cut(s) 633, 1362, 2283, 2430
MaeII ACGT 1 cut(s) 2578
MalI GATC 9 cut(s) 82, 1023, 1143, 1239, 1515, 1806, 2131, 2519, 2685
MbiI CCGCTC 2 cut(s) 1213, 2572
MboI GATC 9 cut(s) 80, 1021, 1141, 1237, 1513, 1804, 2129, 2517, 2683
MfeI CAATTG 1 cut(s) 2723
MflI RGATCY 4 cut(s) 80, 1513, 1804, 2517
MhlI GDGCHC 8 cut(s) 75, 469, 683, 790, 796, 1569, 1820, 2281
MlsI TGGCCA 1 cut(s) 1884
MluNI TGGCCA 1 cut(s) 1884
MlyI GAGTC 6 cut(s) 259, 319, 457, 613, 623, 1543
MmeI TCCRAC 5 cut(s) 108, 378, 1547, 2547, 2646
Mox20I TGGCCA 1 cut(s) 1884
Mph1103I ATGCAT 1 cut(s) 2187
MroNI GCCGGC 2 cut(s) 183, 2243
MscI TGGCCA 1 cut(s) 1884
MseI TTAA 6 cut(s) 837, 1442, 1617, 1841, 2142, 2436
Msp20I TGGCCA 1 cut(s) 1884
MspA1I CMGCKG 5 cut(s) 98, 296, 1206, 1746, 2040
MspI CCGG 5 cut(s) 184, 374, 2244, 2386, 2764
MspR9I CCNGG 8 cut(s) 46, 550, 852, 999, 1059, 1419, 1901, 2765
MunI CAATTG 1 cut(s) 2723
Mva1269I GAATGC 2 cut(s) 148, 1082
MvaI CCWGG 7 cut(s) 46, 550, 852, 999, 1059, 1419, 1901
MwoI GCNNNNNNNGC 2 cut(s) 649, 2485
NaeI GCCGGC 2 cut(s) 185, 2245
NciI CCSGG 1 cut(s) 2765
NdeI CATATG 1 cut(s) 1035
NdeII GATC 9 cut(s) 80, 1021, 1141, 1237, 1513, 1804, 2129, 2517, 2683
NgoMIV GCCGGC 2 cut(s) 183, 2243
NmeAIII GCCGAG 1 cut(s) 138
NmuCI GTSAC 8 cut(s) 425, 494, 1199, 1541, 1874, 2204, 2318, 2362
NsiI ATGCAT 1 cut(s) 2187
NspI RCATGY 8 cut(s) 901, 1467, 1929, 1935, 2105, 2189, 2205, 2307
NspV TTCGAA 1 cut(s) 125
OliI CACNNNNGTG 2 cut(s) 1206, 1860
PaeI GCATGC 1 cut(s) 2189
PaeR7I CTCGAG 2 cut(s) 789, 983
PciI ACATGT 5 cut(s) 897, 1925, 2101, 2201, 2303
PciSI GCTCTTC 1 cut(s) 2732
PctI GAATGC 2 cut(s) 148, 1082
PdiI GCCGGC 2 cut(s) 185, 2245
PfeI GAWTC 8 cut(s) 49, 574, 826, 889, 1519, 1643, 1865, 2411
PfoI TCCNGGA 2 cut(s) 44, 850
PleI GAGTC 6 cut(s) 258, 318, 456, 612, 623, 1543
PpsI GAGTC 6 cut(s) 258, 318, 456, 612, 623, 1543
PpuMI RGGWCCY 2 cut(s) 994, 1229
PscI ACATGT 5 cut(s) 897, 1925, 2101, 2201, 2303
Psp124BI GAGCTC 2 cut(s) 796, 2281
Psp5II RGGWCCY 2 cut(s) 994, 1229
Psp6I CCWGG 7 cut(s) 44, 548, 850, 997, 1057, 1417, 1899
PspFI CCCAGC 2 cut(s) 66, 146
PspGI CCWGG 7 cut(s) 44, 548, 850, 997, 1057, 1417, 1899
PspPPI RGGWCCY 2 cut(s) 994, 1229
PspXI VCTCGAGB 1 cut(s) 789
PstI CTGCAG 2 cut(s) 820, 1751
PstNI CAGNNNCTG 1 cut(s) 2075
PsuI RGATCY 4 cut(s) 80, 1513, 1804, 2517
PvuII CAGCTG 1 cut(s) 2040
RsaI GTAC 4 cut(s) 867, 1972, 2308, 2484
RsaNI GTAC 4 cut(s) 866, 1971, 2307, 2483
SacI GAGCTC 2 cut(s) 796, 2281
SapI GCTCTTC 1 cut(s) 2732
SaqAI TTAA 6 cut(s) 837, 1442, 1617, 1841, 2142, 2436
Sau3AI GATC 9 cut(s) 80, 1021, 1141, 1237, 1513, 1804, 2129, 2517, 2683
SchI GAGTC 6 cut(s) 259, 319, 457, 613, 623, 1543
ScrFI CCNGG 8 cut(s) 46, 550, 852, 999, 1059, 1419, 1901, 2765
SduI GDGCHC 8 cut(s) 75, 469, 683, 790, 796, 1569, 1820, 2281
SfaNI GCATC 8 cut(s) 141, 783, 1124, 2100, 2332, 2581, 2611, 2705
SfcI CTRYAG 4 cut(s) 358, 816, 1747, 2076
Sfr274I CTCGAG 2 cut(s) 789, 983
SfuI TTCGAA 1 cut(s) 125
SinI GGWCC 9 cut(s) 13, 42, 735, 994, 1061, 1229, 2107, 2668, 2767
SlaI CTCGAG 2 cut(s) 789, 983
SmlI CTYRAG 3 cut(s) 742, 789, 983
SmoI CTYRAG 3 cut(s) 742, 789, 983
SphI GCATGC 1 cut(s) 2189
SspMI CTAG 4 cut(s) 633, 1362, 2283, 2430
SstI GAGCTC 2 cut(s) 796, 2281
StyD4I CCNGG 8 cut(s) 44, 548, 850, 997, 1057, 1417, 1899, 2763
StyI CCWWGG 1 cut(s) 2282
TaaI ACNGT 9 cut(s) 303, 431, 685, 1098, 1472, 1880, 1889, 2449, 2647
TaiI ACGT 1 cut(s) 2581
TaqI TCGA 5 cut(s) 125, 755, 790, 984, 2132
TatI WGTACW 2 cut(s) 865, 2306
TauI GCSGC 4 cut(s) 98, 299, 643, 2196
TfiI GAWTC 8 cut(s) 49, 574, 826, 889, 1519, 1643, 1865, 2411
Tru1I TTAA 6 cut(s) 837, 1442, 1617, 1841, 2142, 2436
Tru9I TTAA 6 cut(s) 837, 1442, 1617, 1841, 2142, 2436
TscAI CASTG 9 cut(s) 688, 1055, 1408, 1475, 1577, 1756, 1885, 1892, 2076
TseFI GTSAC 8 cut(s) 425, 494, 1199, 1541, 1874, 2204, 2318, 2362
Tsp45I GTSAC 8 cut(s) 425, 494, 1199, 1541, 1874, 2204, 2318, 2362
TspDTI ATGAA 5 cut(s) 587, 638, 945, 2016, 2568
TspGWI ACGGA 2 cut(s) 1465, 1735
TspRI CASTG 9 cut(s) 688, 1055, 1408, 1475, 1577, 1756, 1885, 1892, 2076
VneI GTGCAC 1 cut(s) 1565
VpaK11BI GGWCC 9 cut(s) 13, 42, 735, 994, 1061, 1229, 2107, 2668, 2767
XapI RAATTY 4 cut(s) 657, 1407, 1694, 2614
XceI RCATGY 8 cut(s) 901, 1467, 1929, 1935, 2105, 2189, 2205, 2307
XcmI CCANNNNNNNNNTGG 1 cut(s) 556
XhoI CTCGAG 2 cut(s) 789, 983
XmaJI CCTAGG 1 cut(s) 2282
XspI CTAG 4 cut(s) 633, 1362, 2283, 2430
Zsp2I ATGCAT 1 cut(s) 2187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.