MD07G1010200.v1.1

histone methyltransferase activity (H3-K4 specific)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0010082
Physical Location & Seq
Reverse (-)
928072 .. 928438
367 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1010200.v1.1.491

Sequence Viewer

Length: 216 bp
ATGGTGTTGCAGTTGCTGTTAACAGTTGCCTTCTCTGCAGTGCCTCTGACTCTGTACGTTCCTCCAGTCAGAAGCCTAAACCCCTTCGTGGAGACCATGGAGGAACTCCTCTCCGAATCAAGAACTCACACCAATAGGGTATACCCACGTGTCCGAGATCTTTGGTTGAGGATCTTGGATTGCTTTCTTTGCAGCAGAACCACAAGGTCTGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

72

Amino Acids

8.29

Weight (kDa)

7.84

Isoelectric Point (pI)

65.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 141
AclWI GGATC 1 cut(s) 179
AcvI CACGTG 1 cut(s) 149
AfaI GTAC 1 cut(s) 56
AfiI CCNNNNNNNGG 1 cut(s) 88
AflIII ACRYGT 1 cut(s) 148
Alw26I GTCTC 1 cut(s) 86
AlwI GGATC 1 cut(s) 179
AlwNI CAGNNNCTG 1 cut(s) 16
ApeKI GCWGC 1 cut(s) 192
BbrPI CACGTG 1 cut(s) 149
BbvI GCAGC 1 cut(s) 204
BcoDI GTCTC 1 cut(s) 86
BfmI CTRYAG 1 cut(s) 36
BglII AGATCT 1 cut(s) 157
BisI GCNGC 1 cut(s) 193
BlsI GCNGC 1 cut(s) 194
BpmI CTGGAG 1 cut(s) 48
BsaAI YACGTR 1 cut(s) 149
BsaI GGTCTC 1 cut(s) 86
BsaJI CCNNGG 1 cut(s) 96
Bsc4I CCNNNNNNNGG 1 cut(s) 88
Bse1I ACTGG 1 cut(s) 65
BseDI CCNNGG 1 cut(s) 96
BseLI CCNNNNNNNGG 1 cut(s) 88
BseMII CTCAG 1 cut(s) 201
BseNI ACTGG 1 cut(s) 65
BseRI GAGGAG 1 cut(s) 98
BseXI GCAGC 1 cut(s) 204
BslI CCNNNNNNNGG 1 cut(s) 88
BsmAI GTCTC 1 cut(s) 86
Bso31I GGTCTC 1 cut(s) 86
Bsp143I GATC 2 cut(s) 157, 171
Bsp19I CCATGG 1 cut(s) 96
BspCNI CTCAG 1 cut(s) 202
BspMAI CTGCAG 1 cut(s) 40
BspPI GGATC 1 cut(s) 179
BspTNI GGTCTC 1 cut(s) 86
BsrI ACTGG 1 cut(s) 65
BssECI CCNNGG 1 cut(s) 96
BssMI GATC 2 cut(s) 157, 171
BssNAI GTATAC 1 cut(s) 142
BssT1I CCWWGG 1 cut(s) 96
Bst1107I GTATAC 1 cut(s) 142
Bst4CI ACNGT 1 cut(s) 25
BstBAI YACGTR 1 cut(s) 149
BstDEI CTNAG 1 cut(s) 210
BstDSI CCRYGG 1 cut(s) 96
BstKTI GATC 2 cut(s) 160, 174
BstMAI GTCTC 1 cut(s) 86
BstMBI GATC 2 cut(s) 157, 171
BstMWI GCNNNNNNNGC 2 cut(s) 35, 189
BstSFI CTRYAG 1 cut(s) 36
BstV1I GCAGC 1 cut(s) 204
BstX2I RGATCY 2 cut(s) 157, 171
BstYI RGATCY 2 cut(s) 157, 171
BstZ17I GTATAC 1 cut(s) 142
BtgI CCRYGG 1 cut(s) 96
BtsI GCAGTG 1 cut(s) 45
BtsIMutI CAGTG 1 cut(s) 45
CaiI CAGNNNCTG 1 cut(s) 16
Csp6I GTAC 1 cut(s) 55
CviAII CATG 1 cut(s) 97
CviJI RGCY 1 cut(s) 75
CviKI_1 RGCY 1 cut(s) 75
CviQI GTAC 1 cut(s) 55
DdeI CTNAG 1 cut(s) 210
DpnI GATC 2 cut(s) 159, 173
DpnII GATC 2 cut(s) 157, 171
Eco130I CCWWGG 1 cut(s) 96
Eco31I GGTCTC 1 cut(s) 86
Eco72I CACGTG 1 cut(s) 149
EcoT14I CCWWGG 1 cut(s) 96
ErhI CCWWGG 1 cut(s) 96
FaeI CATG 1 cut(s) 100
FaiI YATR 2 cut(s) 98, 142
FatI CATG 1 cut(s) 96
FblI GTMKAC 1 cut(s) 141
Fnu4HI GCNGC 1 cut(s) 193
Fsp4HI GCNGC 1 cut(s) 193
GluI GCNGC 1 cut(s) 193
GsuI CTGGAG 1 cut(s) 48
Hin1II CATG 1 cut(s) 100
HincII GTYRAC 1 cut(s) 21
HindII GTYRAC 1 cut(s) 21
HinfI GANTC 2 cut(s) 49, 116
HpaI GTTAAC 1 cut(s) 21
Hpy166II GTNNAC 2 cut(s) 21, 142
Hpy188I TCNGA 5 cut(s) 48, 71, 115, 155, 211
Hpy188III TCNNGA 1 cut(s) 120
Hpy8I GTNNAC 2 cut(s) 21, 142
HpyAV CCTTC 2 cut(s) 40, 94
HpyCH4III ACNGT 1 cut(s) 25
HpyCH4IV ACGT 2 cut(s) 57, 148
HpyCH4V TGCA 3 cut(s) 10, 38, 192
HpyF10VI GCNNNNNNNGC 2 cut(s) 35, 189
HpyF3I CTNAG 1 cut(s) 210
HpySE526I ACGT 2 cut(s) 57, 148
Hsp92II CATG 1 cut(s) 100
KspAI GTTAAC 1 cut(s) 21
Kzo9I GATC 2 cut(s) 157, 171
LpnPI CCDG 1 cut(s) 78
Lsp1109I GCAGC 1 cut(s) 204
MaeII ACGT 2 cut(s) 57, 148
MalI GATC 2 cut(s) 159, 173
MboI GATC 2 cut(s) 157, 171
MflI RGATCY 2 cut(s) 157, 171
MlyI GAGTC 1 cut(s) 43
MnlI CCTC 5 cut(s) 54, 72, 94, 119, 162
MseI TTAA 1 cut(s) 20
MwoI GCNNNNNNNGC 2 cut(s) 35, 189
NcoI CCATGG 1 cut(s) 96
NdeII GATC 2 cut(s) 157, 171
NlaIII CATG 1 cut(s) 100
PfeI GAWTC 1 cut(s) 116
PkrI GCNGC 1 cut(s) 194
PleI GAGTC 1 cut(s) 43
PmaCI CACGTG 1 cut(s) 149
PmlI CACGTG 1 cut(s) 149
PpsI GAGTC 1 cut(s) 43
Ppu21I YACGTR 1 cut(s) 149
PspCI CACGTG 1 cut(s) 149
PstI CTGCAG 1 cut(s) 40
PstNI CAGNNNCTG 1 cut(s) 16
PsuI RGATCY 2 cut(s) 157, 171
RsaI GTAC 1 cut(s) 56
RsaNI GTAC 1 cut(s) 55
SaqAI TTAA 1 cut(s) 20
SatI GCNGC 1 cut(s) 193
Sau3AI GATC 2 cut(s) 157, 171
SchI GAGTC 1 cut(s) 43
SetI ASST 3 cut(s) 60, 151, 209
SfcI CTRYAG 1 cut(s) 36
SgeI CNNG 8 cut(s) 77, 100, 109, 132, 159, 161, 167, 187
StyI CCWWGG 1 cut(s) 96
TaaI ACNGT 1 cut(s) 25
TaiI ACGT 2 cut(s) 60, 151
TfiI GAWTC 1 cut(s) 116
Tru1I TTAA 1 cut(s) 20
Tru9I TTAA 1 cut(s) 20
TscAI CASTG 1 cut(s) 45
TseI GCWGC 1 cut(s) 192
TspRI CASTG 1 cut(s) 45
XmiI GTMKAC 1 cut(s) 141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.