Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0015494

Physical Location & Seq

Genomic Coordinates Forward (+)

3418512 .. 3421097

Gene Structure 2586 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1040900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2499 bp

ATGAGCTCACAGCTCTTATCTTTCATACCTCTACACCCCATAACCAACACCATAACGCCGCCACTTCCACCACCACCGCCACCAACTCCGTCTCGACTCCGCCCTCCAATCGCAACCCCAAAATCACAAACCTTTCACACCCTTTCCCAACGAACCCACATTCCTTCTCATGTCTACAAGCACCCTGCAGCCATCCTCCTCGAGCTCTGCACTTCCGTGCAAGAACTCAATCAAATCATCCCCCTCATCATCAAGAACGGTCTCTACAACGAGCACTTGTTCCAGACTAAGCTTGTCAGCTTGTTCTGCAACTATGGCTGCCCCAATGAGGCCGCTCGGGTTTTCGACACCGTTGAGGACAAACTCGAGGTCTTTTACCACACTCTGCTCAAAGGGTATGCCAAGAACTCGTCTTTAGGTGACGCCTTTTCGTTCTTTTGTCGAATGAAGTATGATGGTGTTAAACCTGTTGTGTATAATTTTACATATTTGTTGAAAGTTTGTGGTGACAATGCTGATCTTAGGAGGGGAAAAGAGATTCATGGGGATCTAATTAGTAGTGGGTTTGCCTCAAATTTGTTTGCGATGACCGCGGTTGTGAATCTGTATGCGAAATGTAGGCAGGTTGATGAGGCATATAAGATGTTTGATAGAATGCCTGAGAGAGACTTGGTTTCTTGGAATACCATTGTTGCTGGTTATGCCCAAAATGGGTTGGCAAAGATAGCTATGGAGTTGGTTATACGGATGCAGGAGGAAGGTCAGAAGCCTGATCCGATCACGTTGGTCACCCTTTTGCCTGCTGTTGCTGATTTTGGGTCTTTGATAATTGGAAAATCAATTCATGCGTATGTTGTAAGAGCTGGTTTCGAATCGCATGTAAACCTTTCTACTGCTCTTGTAGACATGTATTCAAAATGTGGATCGGTGGGGACTGCTCGGTTGATTTTCAATAGGATGAAGCACAAGACTGTTGTTTCTTGGAATTCCATGATAGACGGGTACGTACAAAATGAGGACCCCAAGGAGGCAATGGAGGTTTTCCAGAAGATGTTGGATCAAGGCTTTGAACCAACAAATGTTACTATAATGGAAGCTTTGCATGCTTGTGCTGATTTGGGAGATCTTGAGCGAGGAATGTTCGTGCATAAATTATTGGATCAGATGAAACTCGGATCTGATGTTTCGGTCATGAACTCTTTGATATCCATGTATTCGAAGTGTAAGCGAGTTGACATTGCTGCAAAAATATTTAACAAGTTGCAGGGTAAGACCCCAGTCTCATGGAATGCCATGATATTAGGTTATGCGCAGAATGGGCGCGTCAGCGAGGCTTTAAGTCACTTTTGCGAGATGCATTCGCAAAATATAAAACCAGATTCATTTACAATGGTGAGTGTCATCCCTGCTATCGCGGAGCTATCAGTTACACGACAGGCTAAGTGGATTCATGGACTTGTAATAAGAAAATGTTTTGACAAAAACGTTTTTGTGATGACTGCCCTTGTTGACATGTATGCAAAATGTGGAGCTGTGCACACTGCGAGAAAACTTTTTGACATGATGGATGAGCGGCATGTGACGACATGGAACGCTATGATTGATGGATATGGAACAAATGGGCTCGGAAAAGCTGCTGTGAATCTGTTCAATGAAATGAAAAAAGGAGCAATAAAGCCAAATGATATAACGTTCCTATGTGTGATCTCTGCTTGCAGTCACTCGGGTTTAGTGGAAGAGGGTCTACAATACTTTGCAAGCCTGAAAGAAGATTATGGCTTGGAGCCTGCAATGGATCACTACGGGGCCATGGTTGATCTCCTTGGTCGAGCTGGCCGGCTAAATGAGGCTTGGGATTTTATTCAAAATATGCCTATGGAGCCTGGTATCACCGTCTTCGGAGCCATGTTGGGGGCTTGCAGAATTCATAAAAATATAGAGTTGGGGGAAAAGGCAGCAGATAAGATATTTGAGTTAAATCCTGCGGATGGCGGGTATCATGTTTTGCTTGCTAACATATATCAAACAGCTTCATTGTGGGACAAAGTGGCCAAAGTGCGAACGATGATGGAGAAGAAAGGGCTCCAGAAAACCCCAGGCTGCAGTTTAGTGGATTTGAAGAACGAGGTTCACACTTTCTATTCCGGAAGCACAAGCCATCCCCAATCCAAAAGAATCTATACTTTTCTGGACACACTGGGAGATGAGATCAAGGCGGCTGGTTATGTGCCGGACACCAATTCAATTCATGATGTGGAAGCTGATGTTAAGGAGCAGTTGCTCAATAGCCACAGTGAGAAGCTGGCTATTGCGTTTGGGCTCTTGAACACTACTCCTGGTGCCACCATACACATTCGGAAGAATTTACGAGTTTGCGGAGATTGCCATAACGCAACCAAGTACATTTCCCTTGTAACTAGACGGGAGATCATCGTTCGTGATATGCACCGGTTCCATCATTTCAAGAATGGAACATGTTCGTGTGGCGATTATTGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009507 GO:0009536 GO:0009987 GO:0016070 GO:0016071 GO:0016556 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

833

Amino Acids

92.83

Weight (kDa)

7.96

Isoelectric Point (pI)

31.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	124 - 168	4.5e-06	PPR repeat family
PPR_3	PF13812	125 - 171	1.8e-06	Pentatricopeptide repeat domain
PPR_long	PF17177	128 - 273	1e-05	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	223 - 268	2.6e-07	PPR repeat family
PPR_2	PF13041	324 - 363	4.1e-11	PPR repeat family
PPR	PF01535	326 - 356	1.3e-09	PPR repeat
PPR_2	PF13041	427 - 470	1.7e-10	PPR repeat family
PPR	PF01535	427 - 456	1.1e-07	PPR repeat
PPR_2	PF13041	527 - 573	4.8e-14	PPR repeat family
PPR	PF01535	529 - 555	4.1e-07	PPR repeat
E_motif	PF20431	643 - 704	2.1e-18	E motif
DYW_deaminase	PF14432	741 - 832	6.7e-41	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0015494)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G11290
fragaria_vesca	FvH4_7g04350
malus_domestica	MD07G1040900.v1.1
prunus_persica	Prupe.2G049000_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0329341
rosa_laevigata	RLG00000029901
rosa_multiflora	Rmu_co8161036.1_g000001 Rmu_sc0006184.1_g000011 Rmu_sc0039010.1_g000001
rosa_roxburghii	Rroxscaffold_4G00321210
rosa_rugosa	Rorug01G0078700
rosa_samantha	Rh1AG097000 Rh1BG077600 Rh1DG100500
rosa_wichuraiana	Rw1G007520 Rw1G007580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1311
Acc36I	ACCTGC	1 cut(s)	613
AccB1I	GGYRCC	1 cut(s)	2339
AccBSI	CCGCTC	2 cut(s)	335, 1573
AccI	GTMKAC	3 cut(s)	174, 903, 1744
AccII	CGCG	3 cut(s)	593, 1323, 1415
AccIII	TCCGGA	1 cut(s)	2144
AclI	AACGTT	2 cut(s)	1485, 1691
AclWI	GGATC	7 cut(s)	555, 767, 931, 1065, 1167, 1183, 1803
AcoI	YGGCCR	2 cut(s)	1834, 2049
AcsI	RAATTY	4 cut(s)	574, 985, 1923, 2362
AcyI	GRCGYC	1 cut(s)	423
AfaI	GTAC	3 cut(s)	1004, 1008, 2402
AfiI	CCNNNNNNNGG	5 cut(s)	328, 711, 1027, 1911, 1988
AflIII	ACRYGT	3 cut(s)	906, 1512, 2474
AgeI	ACCGGT	1 cut(s)	2448
AjnI	CCWGG	3 cut(s)	1882, 2095, 2335
AleI	CACNNNNGTG	1 cut(s)	215
AloI	GAACNNNNNNTCC	2 cut(s)	145, 177
Alw21I	GWGCWC	4 cut(s)	8, 207, 276, 1539
Alw26I	GTCTC	4 cut(s)	96, 266, 660, 1285
Alw44I	GTGCAC	1 cut(s)	1535
AlwI	GGATC	7 cut(s)	555, 767, 931, 1065, 1167, 1183, 1803
Ama87I	CYCGRG	4 cut(s)	200, 336, 365, 1723
Aor13HI	TCCGGA	1 cut(s)	2144
AoxI	GGCC	4 cut(s)	330, 1806, 1834, 2049
ApaLI	GTGCAC	1 cut(s)	1535
ApeKI	GCWGC	6 cut(s)	188, 318, 1241, 1634, 1955, 2100
ApoI	RAATTY	4 cut(s)	574, 985, 1923, 2362
ArsI	GACNNNNNNTTYG	2 cut(s)	354, 386
AsiGI	ACCGGT	1 cut(s)	2448
Asp700I	GAANNNNTTC	2 cut(s)	1646, 2476
AspLEI	GCGC	2 cut(s)	1312, 1323
AspS9I	GGNCC	2 cut(s)	1018, 1806
AsuHPI	GGTGA	5 cut(s)	431, 518, 781, 1405, 1882
AsuII	TTCGAA	2 cut(s)	870, 1217
AvaI	CYCGRG	4 cut(s)	200, 336, 365, 1723
AvaII	GGWCC	1 cut(s)	1018
BaeGI	GKGCMC	1 cut(s)	1539
BalI	TGGCCA	1 cut(s)	2051
BanI	GGYRCC	1 cut(s)	2339
BanII	GRGCYC	5 cut(s)	8, 207, 1626, 2085, 2322
BarI	GAAGNNNNNNTAC	2 cut(s)	1728, 1760
BbsI	GAAGAC	1 cut(s)	1888
Bbv12I	GWGCWC	4 cut(s)	8, 207, 276, 1539
BbvI	GCAGC	6 cut(s)	200, 305, 1228, 1621, 1967, 2087
BccI	CCATC	8 cut(s)	200, 449, 1558, 1598, 1982, 2062, 2166, 2463
BciT130I	CCWGG	3 cut(s)	1884, 2097, 2337
BcoDI	GTCTC	4 cut(s)	96, 266, 660, 1285
BfaI	CTAG	1 cut(s)	2418
BfmI	CTRYAG	2 cut(s)	186, 2101
BfuAI	ACCTGC	1 cut(s)	613
BglII	AGATCT	1 cut(s)	1123
Bme1390I	CCNGG	3 cut(s)	1884, 2097, 2337
Bme18I	GGWCC	1 cut(s)	1018
BmeT110I	CYCGRG	4 cut(s)	200, 336, 365, 1723
BmgT120I	GGNCC	2 cut(s)	1018, 1806
BmiI	GGNNCC	8 cut(s)	1020, 1785, 1807, 1881, 1903, 2084, 2341, 2453
BmrFI	CCNGG	3 cut(s)	1884, 2097, 2337
BmrI	ACTGGG	2 cut(s)	1271, 2207
BmsI	GCATC	2 cut(s)	738, 1344
BmuI	ACTGGG	2 cut(s)	1271, 2207
BoxI	GACNNNNGTC	1 cut(s)	1277
BpiI	GAAGAC	1 cut(s)	1888
BpmI	CTGGAG	1 cut(s)	2069
Bpu14I	TTCGAA	2 cut(s)	870, 1217
BpuEI	CTTGAG	1 cut(s)	1148
BsaAI	YACGTR	1 cut(s)	1006
BsaHI	GRCGYC	1 cut(s)	423
BsaI	GGTCTC	1 cut(s)	266
BsaJI	CCNNGG	5 cut(s)	591, 1023, 1809, 1822, 2095
BsaWI	WCCGGW	2 cut(s)	2144, 2448
Bsc4I	CCNNNNNNNGG	5 cut(s)	328, 711, 1027, 1911, 1988
Bse118I	RCCGGY	2 cut(s)	1836, 2448
Bse1I	ACTGG	2 cut(s)	1277, 2202
Bse3DI	GCAATG	3 cut(s)	1038, 1236, 1797
BseAI	TCCGGA	1 cut(s)	2144
BseBI	CCWGG	3 cut(s)	1884, 2097, 2337
BseDI	CCNNGG	5 cut(s)	591, 1023, 1809, 1822, 2095
BseGI	GGATG	8 cut(s)	192, 237, 753, 963, 1401, 1573, 1993, 2158
BseLI	CCNNNNNNNGG	5 cut(s)	328, 711, 1027, 1911, 1988
BseMI	GCAATG	3 cut(s)	1038, 1236, 1797
BseMII	CTCAG	1 cut(s)	651
BseNI	ACTGG	2 cut(s)	1277, 2202
BseRI	GAGGAG	1 cut(s)	188
BseSI	GKGCMC	1 cut(s)	1539
BseXI	GCAGC	6 cut(s)	200, 305, 1228, 1621, 1967, 2087
BsgI	GTGCAG	1 cut(s)	193
Bsh1236I	CGCG	3 cut(s)	593, 1323, 1415
BshFI	GGCC	4 cut(s)	332, 1808, 1836, 2051
BshNI	GGYRCC	1 cut(s)	2339
BshTI	ACCGGT	1 cut(s)	2448
BsiHKAI	GWGCWC	4 cut(s)	8, 207, 276, 1539
BsiHKCI	CYCGRG	4 cut(s)	200, 336, 365, 1723
BsiSI	CCGG	4 cut(s)	1837, 2145, 2231, 2449
BslFI	GGGAC	2 cut(s)	946, 2054
BslI	CCNNNNNNNGG	5 cut(s)	328, 711, 1027, 1911, 1988
BsmAI	GTCTC	4 cut(s)	96, 266, 660, 1285
BsmBI	CGTCTC	1 cut(s)	96
BsmFI	GGGAC	2 cut(s)	946, 2054
BsmI	GAATGC	3 cut(s)	660, 1294, 1357
BsnI	GGCC	4 cut(s)	332, 1808, 1836, 2051
Bso31I	GGTCTC	1 cut(s)	266
BsoBI	CYCGRG	4 cut(s)	200, 336, 365, 1723
Bsp119I	TTCGAA	2 cut(s)	870, 1217
Bsp1286I	GDGCHC	7 cut(s)	8, 207, 276, 1539, 1626, 2085, 2322
Bsp13I	TCCGGA	1 cut(s)	2144
Bsp19I	CCATGG	1 cut(s)	1809
BspANI	GGCC	4 cut(s)	332, 1808, 1836, 2051
BspCNI	CTCAG	1 cut(s)	652
BspEI	TCCGGA	1 cut(s)	2144
BspFNI	CGCG	3 cut(s)	593, 1323, 1415
BspHI	TCATGA	2 cut(s)	1191, 2248
BspLI	GGNNCC	8 cut(s)	1020, 1785, 1807, 1881, 1903, 2084, 2341, 2453
BspMAI	CTGCAG	2 cut(s)	190, 2105
BspMI	ACCTGC	1 cut(s)	613
BspPI	GGATC	7 cut(s)	555, 767, 931, 1065, 1167, 1183, 1803
BspT104I	TTCGAA	2 cut(s)	870, 1217
BspT107I	GGYRCC	1 cut(s)	2339
BspTNI	GGTCTC	1 cut(s)	266
BsrBI	CCGCTC	2 cut(s)	335, 1573
BsrDI	GCAATG	3 cut(s)	1038, 1236, 1797
BsrFI	RCCGGY	2 cut(s)	1836, 2448
BsrI	ACTGG	2 cut(s)	1277, 2202
BssAI	RCCGGY	2 cut(s)	1836, 2448
BssECI	CCNNGG	5 cut(s)	591, 1023, 1809, 1822, 2095
BssNI	GRCGYC	1 cut(s)	423
BssT1I	CCWWGG	3 cut(s)	1023, 1809, 1822
Bst2UI	CCWGG	3 cut(s)	1884, 2097, 2337
Bst4CI	ACNGT	5 cut(s)	260, 352, 973, 1894, 2294
Bst6I	CTCTTC	1 cut(s)	1731
BstACI	GRCGYC	1 cut(s)	423
BstBAI	YACGTR	1 cut(s)	1006
BstBI	TTCGAA	2 cut(s)	870, 1217
BstDEI	CTNAG	4 cut(s)	288, 521, 660, 1440
BstDSI	CCRYGG	2 cut(s)	591, 1809
BstEII	GGTNACC	1 cut(s)	787
BstF5I	GGATG	8 cut(s)	192, 237, 753, 963, 1401, 1573, 1993, 2158
BstFNI	CGCG	3 cut(s)	593, 1323, 1415
BstHHI	GCGC	2 cut(s)	1312, 1323
BstMAI	GTCTC	4 cut(s)	96, 266, 660, 1285
BstMWI	GCNNNNNNNGC	9 cut(s)	306, 315, 590, 701, 725, 1103, 1318, 1879, 2382
BstNI	CCWGG	3 cut(s)	1884, 2097, 2337
BstNSI	RCATGY	6 cut(s)	881, 910, 1106, 1516, 1580, 2478
BstPAI	GACNNNNGTC	1 cut(s)	1277
BstPI	GGTNACC	1 cut(s)	787
BstSCI	CCNGG	3 cut(s)	1882, 2095, 2335
BstSFI	CTRYAG	2 cut(s)	186, 2101
BstSLI	GKGCMC	1 cut(s)	1539
BstSNI	TACGTA	1 cut(s)	1006
BstUI	CGCG	3 cut(s)	593, 1323, 1415
BstV1I	GCAGC	6 cut(s)	200, 305, 1228, 1621, 1967, 2087
BstV2I	GAAGAC	1 cut(s)	1888
BstX2I	RGATCY	3 cut(s)	547, 1123, 1175
BstXI	CCANNNNNNTGG	1 cut(s)	1284
BstYI	RGATCY	3 cut(s)	547, 1123, 1175
BsuRI	GGCC	4 cut(s)	332, 1808, 1836, 2051
BtgI	CCRYGG	2 cut(s)	591, 1809
BtgZI	GCGATG	1 cut(s)	599
BtsCI	GGATG	8 cut(s)	192, 237, 753, 963, 1401, 1573, 1993, 2158
BtsI	GCAGTG	1 cut(s)	1539
BtsIMutI	CAGTG	3 cut(s)	1539, 2195, 2299
BveI	ACCTGC	1 cut(s)	613
CciI	TCATGA	2 cut(s)	1191, 2248
CfoI	GCGC	2 cut(s)	1312, 1323
Cfr10I	RCCGGY	2 cut(s)	1836, 2448
Cfr13I	GGNCC	2 cut(s)	1018, 1806
Cfr42I	CCGCGG	1 cut(s)	594
CseI	GACGC	2 cut(s)	431, 1312
Csp6I	GTAC	3 cut(s)	1003, 1007, 2401
CspAI	ACCGGT	1 cut(s)	2448
CviQI	GTAC	3 cut(s)	1003, 1007, 2401
DdeI	CTNAG	4 cut(s)	288, 521, 660, 1440
EaeI	YGGCCR	2 cut(s)	1834, 2049
Eam1104I	CTCTTC	1 cut(s)	1731
EarI	CTCTTC	1 cut(s)	1731
EciI	GGCGGA	1 cut(s)	89
Ecl136II	GAGCTC	2 cut(s)	6, 205
Eco105I	TACGTA	1 cut(s)	1006
Eco130I	CCWWGG	3 cut(s)	1023, 1809, 1822
Eco24I	GRGCYC	5 cut(s)	8, 207, 1626, 2085, 2322
Eco31I	GGTCTC	1 cut(s)	266
Eco32I	GATATC	1 cut(s)	1206
Eco47I	GGWCC	1 cut(s)	1018
Eco53kI	GAGCTC	2 cut(s)	6, 205
Eco88I	CYCGRG	4 cut(s)	200, 336, 365, 1723
Eco91I	GGTNACC	1 cut(s)	787
EcoICRI	GAGCTC	2 cut(s)	6, 205
EcoO109I	RGGNCCY	1 cut(s)	1018
EcoO65I	GGTNACC	1 cut(s)	787
EcoRI	GAATTC	2 cut(s)	985, 1923
EcoRII	CCWGG	3 cut(s)	1882, 2095, 2335
EcoRV	GATATC	1 cut(s)	1206
EcoT14I	CCWWGG	3 cut(s)	1023, 1809, 1822
EcoT22I	ATGCAT	1 cut(s)	1359
EcoT38I	GRGCYC	5 cut(s)	8, 207, 1626, 2085, 2322
ErhI	CCWWGG	3 cut(s)	1023, 1809, 1822
Esp3I	CGTCTC	1 cut(s)	96
FaqI	GGGAC	2 cut(s)	946, 2054
FauI	CCCGC	1 cut(s)	1985
FblI	GTMKAC	3 cut(s)	174, 903, 1744
FokI	GGATG	8 cut(s)	179, 224, 760, 970, 1388, 1580, 2000, 2145
FriOI	GRGCYC	5 cut(s)	8, 207, 1626, 2085, 2322
FspBI	CTAG	1 cut(s)	2418
FspI	TGCGCA	1 cut(s)	1311
GlaI	GCGC	2 cut(s)	1311, 1322
GsuI	CTGGAG	1 cut(s)	2069
HaeIII	GGCC	4 cut(s)	332, 1808, 1836, 2051
HapII	CCGG	4 cut(s)	1837, 2145, 2231, 2449
HgaI	GACGC	2 cut(s)	431, 1312
HhaI	GCGC	2 cut(s)	1312, 1323
Hin1I	GRCGYC	1 cut(s)	423
Hin6I	GCGC	2 cut(s)	1310, 1321
HinP1I	GCGC	2 cut(s)	1310, 1321
HincII	GTYRAC	2 cut(s)	1234, 1510
HindII	GTYRAC	2 cut(s)	1234, 1510
HindIII	AAGCTT	2 cut(s)	290, 1095
HinfI	GANTC	8 cut(s)	96, 538, 601, 872, 1379, 1447, 1642, 2175
HpaII	CCGG	4 cut(s)	1837, 2145, 2231, 2449
HphI	GGTGA	5 cut(s)	431, 518, 781, 1405, 1882
Hpy166II	GTNNAC	8 cut(s)	175, 883, 904, 1234, 1510, 1537, 1745, 2131
Hpy188I	TCNGA	8 cut(s)	765, 777, 1164, 1175, 1180, 1628, 1901, 2358
Hpy8I	GTNNAC	8 cut(s)	175, 883, 904, 1234, 1510, 1537, 1745, 2131
HpyAV	CCTTC	2 cut(s)	174, 752
HpyCH4III	ACNGT	5 cut(s)	260, 352, 973, 1894, 2294
HpyCH4IV	ACGT	4 cut(s)	782, 1005, 1485, 1691
HpyF10VI	GCNNNNNNNGC	9 cut(s)	306, 315, 590, 701, 725, 1103, 1318, 1879, 2382
HpyF3I	CTNAG	4 cut(s)	288, 521, 660, 1440
HpySE526I	ACGT	4 cut(s)	782, 1005, 1485, 1691
Hsp92I	GRCGYC	1 cut(s)	423
HspAI	GCGC	2 cut(s)	1310, 1321
Kpn2I	TCCGGA	1 cut(s)	2144
KroI	GCCGGC	1 cut(s)	1836
KroNI	GCCGGC	1 cut(s)	1838
KspI	CCGCGG	1 cut(s)	594
LmnI	GCTCC	8 cut(s)	1417, 1529, 1667, 1783, 1879, 1901, 2088, 2272
Lsp1109I	GCAGC	6 cut(s)	200, 305, 1228, 1621, 1967, 2087
LweI	GCATC	2 cut(s)	738, 1344
MaeI	CTAG	1 cut(s)	2418
MaeII	ACGT	4 cut(s)	782, 1005, 1485, 1691
MaeIII	GTNAC	9 cut(s)	419, 506, 787, 1081, 1340, 1426, 1579, 1718, 2413
MbiI	CCGCTC	2 cut(s)	335, 1573
MboII	GAAGA	7 cut(s)	1060, 1748, 1781, 1888, 2086, 2131, 2371
MflI	RGATCY	3 cut(s)	547, 1123, 1175
MhlI	GDGCHC	7 cut(s)	8, 207, 276, 1539, 1626, 2085, 2322
MlsI	TGGCCA	1 cut(s)	2051
MluNI	TGGCCA	1 cut(s)	2051
MlyI	GAGTC	1 cut(s)	90
MmeI	TCCRAC	1 cut(s)	1035
Mox20I	TGGCCA	1 cut(s)	2051
Mph1103I	ATGCAT	1 cut(s)	1359
MroI	TCCGGA	1 cut(s)	2144
MroNI	GCCGGC	1 cut(s)	1836
MroXI	GAANNNNTTC	2 cut(s)	1646, 2476
MscI	TGGCCA	1 cut(s)	2051
MseI	TTAA	5 cut(s)	462, 1254, 1337, 1976, 2268
MslI	CAYNNNNRTG	4 cut(s)	215, 849, 1107, 2479
Msp20I	TGGCCA	1 cut(s)	2051
MspA1I	CMGCKG	1 cut(s)	593
MspI	CCGG	4 cut(s)	1837, 2145, 2231, 2449
MspR9I	CCNGG	3 cut(s)	1884, 2097, 2337
Mva1269I	GAATGC	3 cut(s)	660, 1294, 1357
MvaI	CCWGG	3 cut(s)	1884, 2097, 2337
MvnI	CGCG	3 cut(s)	593, 1323, 1415
MwoI	GCNNNNNNNGC	9 cut(s)	306, 315, 590, 701, 725, 1103, 1318, 1879, 2382
NaeI	GCCGGC	1 cut(s)	1838
NcoI	CCATGG	1 cut(s)	1809
NgoMIV	GCCGGC	1 cut(s)	1836
NlaIV	GGNNCC	8 cut(s)	1020, 1785, 1807, 1881, 1903, 2084, 2341, 2453
NmuCI	GTSAC	6 cut(s)	419, 506, 787, 1340, 1579, 1718
NsbI	TGCGCA	1 cut(s)	1311
NsiI	ATGCAT	1 cut(s)	1359
NspI	RCATGY	6 cut(s)	881, 910, 1106, 1516, 1580, 2478
NspV	TTCGAA	2 cut(s)	870, 1217
OliI	CACNNNNGTG	1 cut(s)	215
PaeI	GCATGC	1 cut(s)	1106
PaeR7I	CTCGAG	2 cut(s)	200, 365
PagI	TCATGA	2 cut(s)	1191, 2248
PciI	ACATGT	3 cut(s)	906, 1512, 2474
PctI	GAATGC	3 cut(s)	660, 1294, 1357
PdiI	GCCGGC	1 cut(s)	1838
PdmI	GAANNNNTTC	2 cut(s)	1646, 2476
PfeI	GAWTC	7 cut(s)	538, 601, 872, 1379, 1447, 1642, 2175
PinAI	ACCGGT	1 cut(s)	2448
PleI	GAGTC	1 cut(s)	90
PpsI	GAGTC	1 cut(s)	90
Ppu21I	YACGTR	1 cut(s)	1006
PpuMI	RGGWCCY	1 cut(s)	1018
PscI	ACATGT	3 cut(s)	906, 1512, 2474
PshAI	GACNNNNGTC	1 cut(s)	1277
Psp124BI	GAGCTC	2 cut(s)	8, 207
Psp1406I	AACGTT	2 cut(s)	1485, 1691
Psp5II	RGGWCCY	1 cut(s)	1018
Psp6I	CCWGG	3 cut(s)	1882, 2095, 2335
PspEI	GGTNACC	1 cut(s)	787
PspGI	CCWGG	3 cut(s)	1882, 2095, 2335
PspN4I	GGNNCC	8 cut(s)	1020, 1785, 1807, 1881, 1903, 2084, 2341, 2453
PspPI	GGNCC	2 cut(s)	1018, 1806
PspPPI	RGGWCCY	1 cut(s)	1018
PspXI	VCTCGAGB	2 cut(s)	200, 365
PsrI	GAACNNNNNNTAC	2 cut(s)	248, 280
PstI	CTGCAG	2 cut(s)	190, 2105
PsuI	RGATCY	3 cut(s)	547, 1123, 1175
RsaI	GTAC	3 cut(s)	1004, 1008, 2402
RsaNI	GTAC	3 cut(s)	1003, 1007, 2401
RseI	CAYNNNNRTG	4 cut(s)	215, 849, 1107, 2479
SacI	GAGCTC	2 cut(s)	8, 207
SacII	CCGCGG	1 cut(s)	594
SaqAI	TTAA	5 cut(s)	462, 1254, 1337, 1976, 2268
Sau96I	GGNCC	2 cut(s)	1018, 1806
SchI	GAGTC	1 cut(s)	90
ScrFI	CCNGG	3 cut(s)	1884, 2097, 2337
SduI	GDGCHC	7 cut(s)	8, 207, 276, 1539, 1626, 2085, 2322
SfaNI	GCATC	2 cut(s)	738, 1344
SfcI	CTRYAG	2 cut(s)	186, 2101
Sfr274I	CTCGAG	2 cut(s)	200, 365
Sfr303I	CCGCGG	1 cut(s)	594
SfuI	TTCGAA	2 cut(s)	870, 1217
SgrBI	CCGCGG	1 cut(s)	594
SinI	GGWCC	1 cut(s)	1018
SlaI	CTCGAG	2 cut(s)	200, 365
SmiMI	CAYNNNNRTG	4 cut(s)	215, 849, 1107, 2479
SmlI	CTYRAG	3 cut(s)	200, 365, 1127
SmoI	CTYRAG	3 cut(s)	200, 365, 1127
SnaBI	TACGTA	1 cut(s)	1006
SphI	GCATGC	1 cut(s)	1106
SspI	AATATT	1 cut(s)	1251
SspMI	CTAG	1 cut(s)	2418
SstI	GAGCTC	2 cut(s)	8, 207
StyD4I	CCNGG	3 cut(s)	1882, 2095, 2335
StyI	CCWWGG	3 cut(s)	1023, 1809, 1822
TaaI	ACNGT	5 cut(s)	260, 352, 973, 1894, 2294
TaiI	ACGT	4 cut(s)	785, 1008, 1488, 1694
TaqI	TCGA	8 cut(s)	94, 201, 345, 366, 442, 870, 1217, 1828
TaqII	GACCGA	1 cut(s)	1177
TatI	WGTACW	1 cut(s)	2400
TauI	GCSGC	4 cut(s)	61, 335, 1576, 2219
TfiI	GAWTC	7 cut(s)	538, 601, 872, 1379, 1447, 1642, 2175
Tru1I	TTAA	5 cut(s)	462, 1254, 1337, 1976, 2268
Tru9I	TTAA	5 cut(s)	462, 1254, 1337, 1976, 2268
TscAI	CASTG	3 cut(s)	1546, 2202, 2299
TseFI	GTSAC	6 cut(s)	419, 506, 787, 1340, 1579, 1718
TseI	GCWGC	6 cut(s)	188, 318, 1241, 1634, 1955, 2100
Tsp45I	GTSAC	6 cut(s)	419, 506, 787, 1340, 1579, 1718
TspGWI	ACGGA	3 cut(s)	78, 205, 760
TspRI	CASTG	3 cut(s)	1546, 2202, 2299
VneI	GTGCAC	1 cut(s)	1535
VpaK11BI	GGWCC	1 cut(s)	1018
XapI	RAATTY	4 cut(s)	574, 985, 1923, 2362
XceI	RCATGY	6 cut(s)	881, 910, 1106, 1516, 1580, 2478
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1030
XhoI	CTCGAG	2 cut(s)	200, 365
XmiI	GTMKAC	3 cut(s)	174, 903, 1744
XmnI	GAANNNNTTC	2 cut(s)	1646, 2476
XspI	CTAG	1 cut(s)	2418
Zsp2I	ATGCAT	1 cut(s)	1359

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.