Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0015494

Physical Location & Seq

Genomic Coordinates Reverse (-)

50466421 .. 50469328

Gene Structure 2908 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00321210.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2466 bp

ATGAGCTCACAGCTCTTGCCAGTCACACCTCTGCTCTCCATAACCAACACCAAAACACCACCACCAATATCAACTAGACTTCCAATACCAACATCAACATTTCACCAGCTTTCCCAAAGAAGCCACATTCCTTCTCATGTCTACAAGCACCCTACAGCCATACTCCTCGAGCTCTGCACTTCCATGAGAGAGCTCCACCAAATCTTCCCCCTCATAATCAAGAACGGTCTCTACAACGAGCACTACTTCCAGACCAAGCTCGTCAGCCTCTTCTGCAAATACGGCAGCCCAATAGAAGCCCACCGGGTTTTCGACGCCGTCGGGGACAAACTCGACGTTTTCTACCACACTCTGCTCAAAGGGTACGCCAAGAACTCCTCTTTAGGGGAGGCCATTTCCTTCTTTTGCCGATTGAAGCATGATGGGGTTGCCCCTGTTGTGTATAACTTTACTTACTTGCTCAAGGCTTGTGGCGATAATGCTGACCTCAGAAGGGGGAAGGAGATTCATGGCTCTCTGATTACCACTGGCTTTGCTACTAATCTGTTTGCGATGACCGGAGTTGTGAATTTGTATGCTAAGTGTAGGCAGATTGATGATGCATATAAGATGTTCGATAGAATGCCTGAGAGGGACTTGGTTTCTTGGAACACTATCGTTGCTGCTTATTCGCAAAATGGGTTGGCCAGGAGAGCTTTGGAGTTGGTTATAAGGATGCAGGAGGAAGGCCAGAGGCCCGATTCCATCACATTGGTCACTGCACTGCCTGCTGTTGCAGATTTTGGGTCCTTGGTGATCGGAAAGTCGATTCATGCCTATGTTATAAGAGCCAGTTTTGAGTCACTTGTGAACATTTCTACCGCTCTTCTAGACATGTATTCGAAATGTGGATCTGTGGCCACTGCTCGGTCGATTTTCAACAGGATGAATCACAAGACTGTTGTTTCCTGGAATTCTATGATGGATGGGTATGTACAAAATGAAGATCCTGAGGAAGCAATGGCGATTTTCCAGAAGATGCTGGATGAAGGCTTTGAGCCAACCAATGTTACTATTATGGAAGCTTTACATGCTTGTGCTGATTTAGGGGATCTTGAGCGGGGAAAGTTTGTGCATAAATTATTGGATCAGCTGAACCTTGGATATGATGTCTCAGTTGTGAATTCTTTGATATCCATGTATTCCAAGTGTAAGAGAGTTGATATCGCTGCAAAATTGTTTAGAGGCTTAAAGGGTAAGACCATCGTCTCATGGAATGCCATGATATTAGGTTTTGCACAGAATGGTCGCGTCAGCGAGGCTTTAAGTCACTTCTGTGAGATGCAATCTCAAAATATAAAGCCAGATTCGTTTACAATGGTGAGTGTAATTCCAGCTCTTGCAGAGTTATCAGTTACACGTCAGGCTAAGTGGATTCATGGACTCGTTGTAAGAAAATGTTTTGACAAAAACGTTTTTGTGATGACTGCTCTTGTTGATATGTACGCAAAATGTGGAGCTGTCCACACTGCGAGAAAACTTTTTGACATGATGGATGACCGGCATGTGACAACATGGAACAGTATGATCGATGGATATGGAACACATGGGCTTGGAAAGGCTGCTGTGGAATTGTTCAATGAAATGAAAAAGGGCATAATAAAGCCAAATGATATAACATTTCTATGTATGATATCCGCTTGTTCTCATTCAGGATTGGTGGAAGATGGTCTTCAATACTTTGAAAGCATGAAGGATGACTATGGTTTGGAGCCTGCAATGGATCACTATGGTGCCATGGTTGATCTTCTTGGTCGAGCTGGCCGACTAAATGAAGCTTGGGATTTTATTCAAAAGATGCCTATGGAACCTGGCATTACAGTCTTTGGTGCAATGTTAGGGGCTTGCAGAATTCATAAAAATGTTGAACTGGGGGAAAAGGCAGCAGATAAAATATTCGAGTTGAACCCTGTTGAAGGTGGGTACCATGTTTTGCTTGCTAACATATATTCCACAGCTTCAATGTGGGACAAAGTGGCCAAAGTGCGAACAACAATGGAGAAGAAAGGGCTCAAGAAAACTCCAGGCTGCAGTTTGGTGGATTTAAAGAACCAAGTTCACACTTTCTATTCTGGAAGTACAAGCCATCCCCAATCCAAGAGAATATATACGTATCTGGAGACACTGGGAGATAAGATCAAGGCTGCTGGTTATGTGCCGGACACAAATTCAATTCATGATGTGGAAGATGATGTCAAGGAGCAATTGCTAAATAGCCATAGTGAGAAGCTGGCTATTGCATTTGGACTCTTGAATACTACCCCGGGTACAACAATACACATTCGAAAGAACCTACGAGTTTGTGGTGATTGCCACAACGCAACCAAGTACATTTCACTAGTAACTGGAAGGGAAATCATAGTGCGTGATATGCATCGGTTCCATCATTTCAGGAATGGAACTTGTTCTTGTGGAGATTATTGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009507 GO:0009536 GO:0009987 GO:0016070 GO:0016071 GO:0016556 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

821

Amino Acids

91.77

Weight (kDa)

8.24

Isoelectric Point (pI)

31.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	212 - 253	1e-06	PPR repeat family
PPR_2	PF13041	313 - 352	1.7e-10	PPR repeat family
PPR	PF01535	315 - 345	2.9e-09	PPR repeat
PPR_2	PF13041	414 - 461	1.1e-11	PPR repeat family
PPR	PF01535	416 - 446	2.2e-07	PPR repeat
PPR_2	PF13041	516 - 562	6.7e-14	PPR repeat family
PPR	PF01535	518 - 544	7.1e-08	PPR repeat
E_motif	PF20431	632 - 693	8.4e-20	E motif
DYW_deaminase	PF14432	730 - 821	1.9e-41	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0015494)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G11290
fragaria_vesca	FvH4_7g04350
malus_domestica	MD07G1040900.v1.1
prunus_persica	Prupe.2G049000_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0329341
rosa_laevigata	RLG00000029901
rosa_multiflora	Rmu_co8161036.1_g000001 Rmu_sc0006184.1_g000011 Rmu_sc0039010.1_g000001
rosa_roxburghii	Rroxscaffold_4G00321210
rosa_rugosa	Rorug01G0078700
rosa_samantha	Rh1AG097000 Rh1BG077600 Rh1DG100500
rosa_wichuraiana	Rw1G007520 Rw1G007580

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	710, 824
Acc65I	GGTACC	1 cut(s)	1962
AccB1I	GGYRCC	2 cut(s)	1772, 1962
AccBSI	CCGCTC	2 cut(s)	863, 1099
AccI	GTMKAC	1 cut(s)	141
AccII	CGCG	1 cut(s)	1290
AciI	CCGC	3 cut(s)	861, 1099, 1677
AclI	AACGTT	1 cut(s)	1452
AclWI	GGATC	5 cut(s)	898, 980, 1098, 1134, 1770
AcoI	YGGCCR	4 cut(s)	684, 897, 1801, 2016
AcsI	RAATTY	5 cut(s)	568, 952, 1162, 1890, 2206
AcyI	GRCGYC	1 cut(s)	315
AfaI	GTAC	7 cut(s)	365, 975, 1484, 1964, 2119, 2308, 2369
AfiI	CCNNNNNNNGG	4 cut(s)	384, 493, 906, 1955
AflIII	ACRYGT	2 cut(s)	873, 1397
AhlI	ACTAGT	1 cut(s)	2377
AjiI	CACGTC	1 cut(s)	1400
AjnI	CCWGG	4 cut(s)	686, 947, 1849, 2062
AleI	CACNNNNGTG	2 cut(s)	1314, 1770
Alw21I	GWGCWC	4 cut(s)	8, 174, 195, 243
Alw26I	GTCTC	4 cut(s)	233, 1156, 1252, 2153
AlwI	GGATC	5 cut(s)	898, 980, 1098, 1134, 1770
Ama87I	CYCGRG	2 cut(s)	167, 2302
AoxI	GGCC	7 cut(s)	390, 684, 727, 734, 897, 1801, 2016
ApeKI	GCWGC	7 cut(s)	285, 662, 1208, 1601, 1922, 2067, 2183
ApoI	RAATTY	5 cut(s)	568, 952, 1162, 1890, 2206
Asp700I	GAANNNNTTC	1 cut(s)	2443
Asp718I	GGTACC	1 cut(s)	1962
AspS9I	GGNCC	2 cut(s)	735, 786
AsuC2I	CCSGG	3 cut(s)	305, 2303, 2304
AsuHPI	GGTGA	4 cut(s)	95, 805, 1372, 2357
AsuII	TTCGAA	2 cut(s)	881, 2323
AvaI	CYCGRG	2 cut(s)	167, 2302
AvaII	GGWCC	1 cut(s)	786
AxyI	CCTNAGG	1 cut(s)	990
BalI	TGGCCA	3 cut(s)	686, 899, 2018
BanI	GGYRCC	2 cut(s)	1772, 1962
BanII	GRGCYC	4 cut(s)	8, 174, 195, 2052
BbsI	GAAGAC	1 cut(s)	1703
Bbv12I	GWGCWC	4 cut(s)	8, 174, 195, 243
BbvI	GCAGC	7 cut(s)	297, 649, 1195, 1588, 1934, 2054, 2170
BceAI	ACGGC	2 cut(s)	298, 302
BciT130I	CCWGG	4 cut(s)	688, 949, 1851, 2064
BcnI	CCSGG	3 cut(s)	305, 2303, 2304
BcoDI	GTCTC	4 cut(s)	233, 1156, 1252, 2153
BcuI	ACTAGT	1 cut(s)	2377
BfaI	CTAG	3 cut(s)	75, 869, 2378
BfmI	CTRYAG	2 cut(s)	153, 2068
BisI	GCNGC	7 cut(s)	286, 663, 1209, 1602, 1923, 2068, 2184
BlsI	GCNGC	7 cut(s)	287, 664, 1210, 1603, 1924, 2069, 2185
Bme1390I	CCNGG	7 cut(s)	305, 688, 949, 1851, 2064, 2303, 2304
Bme18I	GGWCC	1 cut(s)	786
BmeT110I	CYCGRG	2 cut(s)	167, 2302
BmgBI	CACGTC	1 cut(s)	1400
BmgT120I	GGNCC	2 cut(s)	735, 786
BmiI	GGNNCC	6 cut(s)	787, 1752, 1774, 1848, 1964, 2420
BmrFI	CCNGG	7 cut(s)	305, 688, 949, 1851, 2064, 2303, 2304
BmrI	ACTGGG	2 cut(s)	1919, 2174
BmsI	GCATC	6 cut(s)	589, 705, 1008, 1311, 1827, 2422
BmuI	ACTGGG	2 cut(s)	1919, 2174
BoxI	GACNNNNGTC	1 cut(s)	1244
BpiI	GAAGAC	1 cut(s)	1703
BpmI	CTGGAG	2 cut(s)	2046, 2177
Bpu14I	TTCGAA	2 cut(s)	881, 2323
BpuEI	CTTGAG	3 cut(s)	446, 1115, 2036
BpuMI	CCSGG	3 cut(s)	305, 2303, 2304
Bsa29I	ATCGAT	1 cut(s)	1569
BsaAI	YACGTR	1 cut(s)	2151
BsaBI	GATNNNNATC	1 cut(s)	2412
BsaHI	GRCGYC	1 cut(s)	315
BsaI	GGTCTC	1 cut(s)	233
BsaJI	CCNNGG	5 cut(s)	789, 1138, 1776, 2301, 2302
BsaWI	WCCGGW	1 cut(s)	557
Bsc4I	CCNNNNNNNGG	4 cut(s)	384, 493, 906, 1955
Bse118I	RCCGGY	1 cut(s)	1539
Bse1I	ACTGG	6 cut(s)	20, 532, 831, 1914, 2169, 2389
Bse21I	CCTNAGG	1 cut(s)	990
Bse3DI	GCAATG	3 cut(s)	1005, 1764, 1878
Bse8I	GATNNNNATC	1 cut(s)	2412
BseBI	CCWGG	4 cut(s)	688, 949, 1851, 2064
BseCI	ATCGAT	1 cut(s)	1569
BseDI	CCNNGG	5 cut(s)	789, 1138, 1776, 2301, 2302
BseGI	GGATG	7 cut(s)	720, 930, 970, 1030, 1540, 1741, 2125
BseJI	GATNNNNATC	1 cut(s)	2412
BseLI	CCNNNNNNNGG	4 cut(s)	384, 493, 906, 1955
BseMI	GCAATG	3 cut(s)	1005, 1764, 1878
BseMII	CTCAG	4 cut(s)	502, 618, 981, 1167
BseNI	ACTGG	6 cut(s)	20, 532, 831, 1914, 2169, 2389
BseRI	GAGGAG	2 cut(s)	155, 367
BseXI	GCAGC	7 cut(s)	297, 649, 1195, 1588, 1934, 2054, 2170
BsgI	GTGCAG	2 cut(s)	160, 744
Bsh1236I	CGCG	1 cut(s)	1290
Bsh1285I	CGRYCG	1 cut(s)	911
BshFI	GGCC	7 cut(s)	392, 686, 729, 736, 899, 1803, 2018
BshNI	GGYRCC	2 cut(s)	1772, 1962
BshVI	ATCGAT	1 cut(s)	1569
BsiEI	CGRYCG	1 cut(s)	911
BsiHKAI	GWGCWC	4 cut(s)	8, 174, 195, 243
BsiHKCI	CYCGRG	2 cut(s)	167, 2302
BsiSI	CCGG	5 cut(s)	304, 558, 1540, 2198, 2303
BslFI	GGGAC	3 cut(s)	338, 647, 2021
BslI	CCNNNNNNNGG	4 cut(s)	384, 493, 906, 1955
BsmAI	GTCTC	4 cut(s)	233, 1156, 1252, 2153
BsmBI	CGTCTC	1 cut(s)	1252
BsmFI	GGGAC	3 cut(s)	338, 647, 2021
BsmI	GAATGC	2 cut(s)	627, 1261
BsnI	GGCC	7 cut(s)	392, 686, 729, 736, 899, 1803, 2018
Bso31I	GGTCTC	1 cut(s)	233
BsoBI	CYCGRG	2 cut(s)	167, 2302
Bsp119I	TTCGAA	2 cut(s)	881, 2323
Bsp1286I	GDGCHC	5 cut(s)	8, 174, 195, 243, 2052
Bsp1407I	TGTACA	1 cut(s)	973
Bsp143I	GATC	9 cut(s)	795, 890, 985, 1090, 1126, 1566, 1762, 1783, 2175
Bsp19I	CCATGG	1 cut(s)	1776
BspACI	CCGC	3 cut(s)	861, 1099, 1677
BspANI	GGCC	7 cut(s)	392, 686, 729, 736, 899, 1803, 2018
BspCNI	CTCAG	4 cut(s)	501, 619, 982, 1166
BspDI	ATCGAT	1 cut(s)	1569
BspFNI	CGCG	1 cut(s)	1290
BspHI	TCATGA	1 cut(s)	2215
BspLI	GGNNCC	6 cut(s)	787, 1752, 1774, 1848, 1964, 2420
BspMAI	CTGCAG	1 cut(s)	2072
BspPI	GGATC	5 cut(s)	898, 980, 1098, 1134, 1770
BspQI	GCTCTTC	1 cut(s)	870
BspT104I	TTCGAA	2 cut(s)	881, 2323
BspT107I	GGYRCC	2 cut(s)	1772, 1962
BspTNI	GGTCTC	1 cut(s)	233
BsrBI	CCGCTC	2 cut(s)	863, 1099
BsrDI	GCAATG	3 cut(s)	1005, 1764, 1878
BsrFI	RCCGGY	1 cut(s)	1539
BsrGI	TGTACA	1 cut(s)	973
BsrI	ACTGG	6 cut(s)	20, 532, 831, 1914, 2169, 2389
BssAI	RCCGGY	1 cut(s)	1539
BssECI	CCNNGG	5 cut(s)	789, 1138, 1776, 2301, 2302
BssMI	GATC	9 cut(s)	795, 890, 985, 1090, 1126, 1566, 1762, 1783, 2175
BssNI	GRCGYC	1 cut(s)	315
BssT1I	CCWWGG	3 cut(s)	789, 1138, 1776
Bst2UI	CCWGG	4 cut(s)	688, 949, 1851, 2064
Bst4CI	ACNGT	4 cut(s)	227, 940, 1562, 1861
Bst6I	CTCTTC	2 cut(s)	275, 870
BstACI	GRCGYC	1 cut(s)	315
BstAPI	GCANNNNNTGC	1 cut(s)	767
BstAUI	TGTACA	1 cut(s)	973
BstBAI	YACGTR	1 cut(s)	2151
BstBI	TTCGAA	2 cut(s)	881, 2323
BstC8I	GCNNGC	6 cut(s)	768, 1755, 1801, 1885, 1977, 2271
BstDEI	CTNAG	6 cut(s)	488, 579, 627, 990, 1153, 1407
BstDSI	CCRYGG	1 cut(s)	1776
BstENI	CCTNNNNNAGG	1 cut(s)	1953
BstF5I	GGATG	7 cut(s)	720, 930, 970, 1030, 1540, 1741, 2125
BstFNI	CGCG	1 cut(s)	1290
BstKTI	GATC	9 cut(s)	798, 893, 988, 1093, 1129, 1569, 1765, 1786, 2178
BstMAI	GTCTC	4 cut(s)	233, 1156, 1252, 2153
BstMBI	GATC	9 cut(s)	795, 890, 985, 1090, 1126, 1566, 1762, 1783, 2175
BstMCI	CGRYCG	1 cut(s)	911
BstMWI	GCNNNNNNNGC	6 cut(s)	273, 282, 692, 767, 1070, 2410
BstNI	CCWGG	4 cut(s)	688, 949, 1851, 2064
BstNSI	RCATGY	3 cut(s)	877, 1073, 1547
BstPAI	GACNNNNGTC	1 cut(s)	1244
BstSCI	CCNGG	7 cut(s)	303, 686, 947, 1849, 2062, 2301, 2302
BstSFI	CTRYAG	2 cut(s)	153, 2068
BstSNI	TACGTA	1 cut(s)	2151
BstUI	CGCG	1 cut(s)	1290
BstV1I	GCAGC	7 cut(s)	297, 649, 1195, 1588, 1934, 2054, 2170
BstV2I	GAAGAC	1 cut(s)	1703
BstX2I	RGATCY	3 cut(s)	890, 985, 1090
BstXI	CCANNNNNNTGG	1 cut(s)	751
BstYI	RGATCY	3 cut(s)	890, 985, 1090
Bsu15I	ATCGAT	1 cut(s)	1569
Bsu36I	CCTNAGG	1 cut(s)	990
BsuRI	GGCC	7 cut(s)	392, 686, 729, 736, 899, 1803, 2018
BsuTUI	ATCGAT	1 cut(s)	1569
BtgI	CCRYGG	1 cut(s)	1776
BtgZI	GCGATG	1 cut(s)	566
BtrI	CACGTC	1 cut(s)	1400
BtsCI	GGATG	7 cut(s)	720, 930, 970, 1030, 1540, 1741, 2125
BtsI	GCAGTG	4 cut(s)	756, 761, 900, 1506
BtsIMutI	CAGTG	6 cut(s)	525, 756, 761, 900, 1506, 2162
Cac8I	GCNNGC	6 cut(s)	768, 1755, 1801, 1885, 1977, 2271
CciI	TCATGA	1 cut(s)	2215
Cfr10I	RCCGGY	1 cut(s)	1539
Cfr13I	GGNCC	2 cut(s)	735, 786
Cfr9I	CCCGGG	1 cut(s)	2302
ClaI	ATCGAT	1 cut(s)	1569
CseI	GACGC	2 cut(s)	323, 1279
Csp6I	GTAC	7 cut(s)	364, 974, 1483, 1963, 2118, 2307, 2368
CspCI	CAANNNNNGTGG	4 cut(s)	451, 486, 514, 549
CviQI	GTAC	7 cut(s)	364, 974, 1483, 1963, 2118, 2307, 2368
DdeI	CTNAG	6 cut(s)	488, 579, 627, 990, 1153, 1407
DpnI	GATC	9 cut(s)	797, 892, 987, 1092, 1128, 1568, 1764, 1785, 2177
DpnII	GATC	9 cut(s)	795, 890, 985, 1090, 1126, 1566, 1762, 1783, 2175
DraI	TTTAAA	1 cut(s)	2085
EaeI	YGGCCR	4 cut(s)	684, 897, 1801, 2016
Eam1104I	CTCTTC	2 cut(s)	275, 870
EarI	CTCTTC	2 cut(s)	275, 870
Ecl136II	GAGCTC	3 cut(s)	6, 172, 193
Eco105I	TACGTA	1 cut(s)	2151
Eco130I	CCWWGG	3 cut(s)	789, 1138, 1776
Eco24I	GRGCYC	4 cut(s)	8, 174, 195, 2052
Eco31I	GGTCTC	1 cut(s)	233
Eco32I	GATATC	3 cut(s)	1173, 1204, 1674
Eco47I	GGWCC	1 cut(s)	786
Eco53kI	GAGCTC	3 cut(s)	6, 172, 193
Eco81I	CCTNAGG	1 cut(s)	990
Eco88I	CYCGRG	2 cut(s)	167, 2302
EcoICRI	GAGCTC	3 cut(s)	6, 172, 193
EcoNI	CCTNNNNNAGG	1 cut(s)	1953
EcoO109I	RGGNCCY	1 cut(s)	786
EcoRI	GAATTC	3 cut(s)	952, 1162, 1890
EcoRII	CCWGG	4 cut(s)	686, 947, 1849, 2062
EcoRV	GATATC	3 cut(s)	1173, 1204, 1674
EcoT14I	CCWWGG	3 cut(s)	789, 1138, 1776
EcoT22I	ATGCAT	2 cut(s)	604, 2415
EcoT38I	GRGCYC	4 cut(s)	8, 174, 195, 2052
ErhI	CCWWGG	3 cut(s)	789, 1138, 1776
Esp3I	CGTCTC	1 cut(s)	1252
FalI	AAGNNNNNCTT	2 cut(s)	1695, 1727
FaqI	GGGAC	3 cut(s)	338, 647, 2021
FauI	CCCGC	1 cut(s)	1092
FblI	GTMKAC	1 cut(s)	141
Fnu4HI	GCNGC	7 cut(s)	286, 663, 1209, 1602, 1923, 2068, 2184
FokI	GGATG	7 cut(s)	727, 937, 977, 1037, 1547, 1748, 2112
FriOI	GRGCYC	4 cut(s)	8, 174, 195, 2052
Fsp4HI	GCNGC	7 cut(s)	286, 663, 1209, 1602, 1923, 2068, 2184
FspBI	CTAG	3 cut(s)	75, 869, 2378
GluI	GCNGC	7 cut(s)	286, 663, 1209, 1602, 1923, 2068, 2184
GsuI	CTGGAG	2 cut(s)	2046, 2177
HaeIII	GGCC	7 cut(s)	392, 686, 729, 736, 899, 1803, 2018
HapII	CCGG	5 cut(s)	304, 558, 1540, 2198, 2303
HgaI	GACGC	2 cut(s)	323, 1279
Hin1I	GRCGYC	1 cut(s)	315
HindIII	AAGCTT	2 cut(s)	1062, 1815
HinfI	GANTC	9 cut(s)	505, 740, 808, 839, 928, 1346, 1414, 1422, 2286
HpaII	CCGG	5 cut(s)	304, 558, 1540, 2198, 2303
HphI	GGTGA	4 cut(s)	95, 805, 1372, 2357
Hpy166II	GTNNAC	5 cut(s)	142, 850, 1353, 1504, 2098
Hpy188I	TCNGA	3 cut(s)	491, 519, 800
Hpy8I	GTNNAC	5 cut(s)	142, 850, 1353, 1504, 2098
Hpy99I	CGWCG	3 cut(s)	317, 323, 338
HpyAV	CCTTC	9 cut(s)	141, 409, 486, 493, 719, 1022, 1726, 1949, 2382
HpyCH4III	ACNGT	4 cut(s)	227, 940, 1562, 1861
HpyCH4IV	ACGT	4 cut(s)	336, 1399, 1452, 2150
HpyF10VI	GCNNNNNNNGC	6 cut(s)	273, 282, 692, 767, 1070, 2410
HpyF3I	CTNAG	6 cut(s)	488, 579, 627, 990, 1153, 1407
HpySE526I	ACGT	4 cut(s)	336, 1399, 1452, 2150
Hsp92I	GRCGYC	1 cut(s)	315
KpnI	GGTACC	1 cut(s)	1966
Kzo9I	GATC	9 cut(s)	795, 890, 985, 1090, 1126, 1566, 1762, 1783, 2175
LguI	GCTCTTC	1 cut(s)	870
LmnI	GCTCC	4 cut(s)	198, 1496, 1750, 2239
Lsp1109I	GCAGC	7 cut(s)	297, 649, 1195, 1588, 1934, 2054, 2170
LweI	GCATC	6 cut(s)	589, 705, 1008, 1311, 1827, 2422
MaeI	CTAG	3 cut(s)	75, 869, 2378
MaeII	ACGT	4 cut(s)	336, 1399, 1452, 2150
MaeIII	GTNAC	8 cut(s)	22, 754, 840, 1048, 1307, 1393, 1546, 2380
MalI	GATC	9 cut(s)	797, 892, 987, 1092, 1128, 1568, 1764, 1785, 2177
MbiI	CCGCTC	2 cut(s)	863, 1099
MboI	GATC	9 cut(s)	795, 890, 985, 1090, 1126, 1566, 1762, 1783, 2175
MfeI	CAATTG	1 cut(s)	2243
MflI	RGATCY	3 cut(s)	890, 985, 1090
MhlI	GDGCHC	5 cut(s)	8, 174, 195, 243, 2052
MlsI	TGGCCA	3 cut(s)	686, 899, 2018
MluNI	TGGCCA	3 cut(s)	686, 899, 2018
MlyI	GAGTC	3 cut(s)	848, 1416, 2280
Mox20I	TGGCCA	3 cut(s)	686, 899, 2018
Mph1103I	ATGCAT	2 cut(s)	604, 2415
MroXI	GAANNNNTTC	1 cut(s)	2443
MscI	TGGCCA	3 cut(s)	686, 899, 2018
MseI	TTAA	3 cut(s)	1229, 1304, 2084
MslI	CAYNNNNRTG	7 cut(s)	182, 816, 1074, 1314, 1663, 1770, 1899
Msp20I	TGGCCA	3 cut(s)	686, 899, 2018
MspA1I	CMGCKG	1 cut(s)	1132
MspI	CCGG	5 cut(s)	304, 558, 1540, 2198, 2303
MspR9I	CCNGG	7 cut(s)	305, 688, 949, 1851, 2064, 2303, 2304
MunI	CAATTG	1 cut(s)	2243
Mva1269I	GAATGC	2 cut(s)	627, 1261
MvaI	CCWGG	4 cut(s)	688, 949, 1851, 2064
MvnI	CGCG	1 cut(s)	1290
MwoI	GCNNNNNNNGC	6 cut(s)	273, 282, 692, 767, 1070, 2410
NciI	CCSGG	3 cut(s)	305, 2303, 2304
NcoI	CCATGG	1 cut(s)	1776
NdeII	GATC	9 cut(s)	795, 890, 985, 1090, 1126, 1566, 1762, 1783, 2175
NlaIV	GGNNCC	6 cut(s)	787, 1752, 1774, 1848, 1964, 2420
NmuCI	GTSAC	5 cut(s)	22, 754, 840, 1307, 1546
NsiI	ATGCAT	2 cut(s)	604, 2415
NspI	RCATGY	3 cut(s)	877, 1073, 1547
NspV	TTCGAA	2 cut(s)	881, 2323
OliI	CACNNNNGTG	2 cut(s)	1314, 1770
PaeR7I	CTCGAG	1 cut(s)	167
PagI	TCATGA	1 cut(s)	2215
PciI	ACATGT	1 cut(s)	873
PciSI	GCTCTTC	1 cut(s)	870
PcsI	WCGNNNNNNNCGW	1 cut(s)	1801
PctI	GAATGC	2 cut(s)	627, 1261
PdmI	GAANNNNTTC	1 cut(s)	2443
PfeI	GAWTC	6 cut(s)	505, 740, 808, 928, 1346, 1414
PflFI	GACNNNGTC	1 cut(s)	317
PfoI	TCCNGGA	1 cut(s)	947
PkrI	GCNGC	7 cut(s)	287, 664, 1210, 1603, 1924, 2069, 2185
PleI	GAGTC	3 cut(s)	847, 1416, 2280
PpsI	GAGTC	3 cut(s)	847, 1416, 2280
Ppu21I	YACGTR	1 cut(s)	2151
PpuMI	RGGWCCY	1 cut(s)	786
PscI	ACATGT	1 cut(s)	873
PshAI	GACNNNNGTC	1 cut(s)	1244
PsiI	TTATAA	2 cut(s)	710, 824
Psp124BI	GAGCTC	3 cut(s)	8, 174, 195
Psp1406I	AACGTT	1 cut(s)	1452
Psp5II	RGGWCCY	1 cut(s)	786
Psp6I	CCWGG	4 cut(s)	686, 947, 1849, 2062
PspGI	CCWGG	4 cut(s)	686, 947, 1849, 2062
PspN4I	GGNNCC	6 cut(s)	787, 1752, 1774, 1848, 1964, 2420
PspPI	GGNCC	2 cut(s)	735, 786
PspPPI	RGGWCCY	1 cut(s)	786
PspXI	VCTCGAGB	1 cut(s)	167
PsrI	GAACNNNNNNTAC	2 cut(s)	215, 247
PstI	CTGCAG	1 cut(s)	2072
PsuI	RGATCY	3 cut(s)	890, 985, 1090
PsyI	GACNNNGTC	1 cut(s)	317
PvuII	CAGCTG	1 cut(s)	1132
RsaI	GTAC	7 cut(s)	365, 975, 1484, 1964, 2119, 2308, 2369
RsaNI	GTAC	7 cut(s)	364, 974, 1483, 1963, 2118, 2307, 2368
RseI	CAYNNNNRTG	7 cut(s)	182, 816, 1074, 1314, 1663, 1770, 1899
SacI	GAGCTC	3 cut(s)	8, 174, 195
SapI	GCTCTTC	1 cut(s)	870
SaqAI	TTAA	3 cut(s)	1229, 1304, 2084
SatI	GCNGC	7 cut(s)	286, 663, 1209, 1602, 1923, 2068, 2184
Sau3AI	GATC	9 cut(s)	795, 890, 985, 1090, 1126, 1566, 1762, 1783, 2175
Sau96I	GGNCC	2 cut(s)	735, 786
SchI	GAGTC	3 cut(s)	848, 1416, 2280
ScrFI	CCNGG	7 cut(s)	305, 688, 949, 1851, 2064, 2303, 2304
SduI	GDGCHC	5 cut(s)	8, 174, 195, 243, 2052
SfaNI	GCATC	6 cut(s)	589, 705, 1008, 1311, 1827, 2422
SfcI	CTRYAG	2 cut(s)	153, 2068
Sfr274I	CTCGAG	1 cut(s)	167
SfuI	TTCGAA	2 cut(s)	881, 2323
SinI	GGWCC	1 cut(s)	786
SlaI	CTCGAG	1 cut(s)	167
SmaI	CCCGGG	1 cut(s)	2304
SmiMI	CAYNNNNRTG	7 cut(s)	182, 816, 1074, 1314, 1663, 1770, 1899
SmlI	CTYRAG	4 cut(s)	167, 461, 1094, 2051
SmoI	CTYRAG	4 cut(s)	167, 461, 1094, 2051
SnaBI	TACGTA	1 cut(s)	2151
SpeI	ACTAGT	1 cut(s)	2377
SsiI	CCGC	3 cut(s)	861, 1099, 1677
SspI	AATATT	1 cut(s)	1935
SspMI	CTAG	3 cut(s)	75, 869, 2378
SstI	GAGCTC	3 cut(s)	8, 174, 195
StyD4I	CCNGG	7 cut(s)	303, 686, 947, 1849, 2062, 2301, 2302
StyI	CCWWGG	3 cut(s)	789, 1138, 1776
TaaI	ACNGT	4 cut(s)	227, 940, 1562, 1861
TaiI	ACGT	4 cut(s)	339, 1402, 1455, 2153
TaqII	GACCGA	1 cut(s)	897
TatI	WGTACW	3 cut(s)	973, 2117, 2367
TfiI	GAWTC	6 cut(s)	505, 740, 808, 928, 1346, 1414
Tru1I	TTAA	3 cut(s)	1229, 1304, 2084
Tru9I	TTAA	3 cut(s)	1229, 1304, 2084
TscAI	CASTG	6 cut(s)	532, 763, 768, 907, 1513, 2169
TseFI	GTSAC	5 cut(s)	22, 754, 840, 1307, 1546
TseI	GCWGC	7 cut(s)	285, 662, 1208, 1601, 1922, 2067, 2183
Tsp45I	GTSAC	5 cut(s)	22, 754, 840, 1307, 1546
TspMI	CCCGGG	1 cut(s)	2302
TspRI	CASTG	6 cut(s)	532, 763, 768, 907, 1513, 2169
Tth111I	GACNNNGTC	1 cut(s)	317
VpaK11BI	GGWCC	1 cut(s)	786
XagI	CCTNNNNNAGG	1 cut(s)	1953
XapI	RAATTY	5 cut(s)	568, 952, 1162, 1890, 2206
XbaI	TCTAGA	1 cut(s)	868
XceI	RCATGY	3 cut(s)	877, 1073, 1547
XcmI	CCANNNNNNNNNTGG	1 cut(s)	694
XhoI	CTCGAG	1 cut(s)	167
XmaI	CCCGGG	1 cut(s)	2302
XmiI	GTMKAC	1 cut(s)	141
XmnI	GAANNNNTTC	1 cut(s)	2443
XspI	CTAG	3 cut(s)	75, 869, 2378
Zsp2I	ATGCAT	2 cut(s)	604, 2415

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.