MD07G1143300.v1.1

Protein CURVATURE THYLAKOID 1A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0005869
Physical Location & Seq
Reverse (-)
20894524 .. 20897202
2679 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1143300.v1.1.491

Sequence Viewer

Length: 489 bp
ATGGCAGCGACAGCCTCAACCATGGCGGCCACCGCCGTCTTCATCCCACGTTTCCCCACCAAACCCACCTGCTACTCCGCCTTGCCTTACCTCCCTCCTCGCCGCTCCTCCTCCCCTTCTTCCTTCCCTCTTAAATCCTTCTCAGGGTCCCGGAGGCTTTCTCTTCCGGTCAGAGCTTCATCTTCCGACGAGACATCCACATCCGTCGACACTGGTGAACTTTTATCAGACTTGAAGGAGAAGTGGGATGCAGTTGAAAATAAGTCTACAGTACTTGTATATGGAGGTGGGGCGATAGTCGCTGTGTGGTTGTCCTCAATAGTTGTTGGCGCCGTCAATTCAGTGCCACTTCTTCCAAAGGTCCTGGAATTGGTAGGGCTAGGATACACTGGATGGTTTGTCTACCGTTACCTACTCTTCAAGTCAAGCAGAAAAGAACTAGCTACAGACATTGAAGCACTGAAGAAAAAGATTGCTGGAACTGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CAAD

Protein Analysis

163

Amino Acids

17.42

Weight (kDa)

9.52

Isoelectric Point (pI)

38.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAAD PF14159 77 - 160 6.7e-32 CAAD domains of cyanobacterial aminoacyl-tRNA synthetase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 77
Acc36I ACCTGC 1 cut(s) 77
AccB1I GGYRCC 1 cut(s) 329
AccBSI CCGCTC 1 cut(s) 105
AccI GTMKAC 3 cut(s) 207, 266, 402
AciI CCGC 4 cut(s) 26, 33, 78, 103
AcoI YGGCCR 1 cut(s) 27
AcuI CTGAAG 1 cut(s) 482
AcyI GRCGYC 1 cut(s) 330
AfaI GTAC 1 cut(s) 273
AfiI CCNNNNNNNGG 2 cut(s) 144, 370
AgsI TTSAA 4 cut(s) 235, 257, 421, 455
AjnI CCWGG 1 cut(s) 363
AluBI AGCT 2 cut(s) 176, 443
AluI AGCT 2 cut(s) 176, 443
Alw26I GTCTC 1 cut(s) 185
AoxI GGCC 1 cut(s) 27
ApeKI GCWGC 1 cut(s) 5
AspLEI GCGC 1 cut(s) 332
AspS9I GGNCC 2 cut(s) 147, 361
AsuC2I CCSGG 1 cut(s) 151
AsuHPI GGTGA 1 cut(s) 227
AvaII GGWCC 2 cut(s) 147, 361
BanI GGYRCC 1 cut(s) 329
BbsI GAAGAC 1 cut(s) 31
BbvI GCAGC 1 cut(s) 17
BccI CCATC 1 cut(s) 387
BceAI ACGGC 2 cut(s) 20, 317
BciT130I CCWGG 1 cut(s) 365
BciVI GTATCC 1 cut(s) 377
BcnI CCSGG 1 cut(s) 151
BcoDI GTCTC 1 cut(s) 185
BfaI CTAG 2 cut(s) 380, 440
BfmI CTRYAG 2 cut(s) 267, 444
BfoI RGCGCY 1 cut(s) 333
BfuAI ACCTGC 1 cut(s) 77
BfuI GTATCC 1 cut(s) 377
BisI GCNGC 3 cut(s) 6, 27, 103
BlsI GCNGC 3 cut(s) 7, 28, 104
BmcAI AGTACT 1 cut(s) 273
Bme1390I CCNGG 2 cut(s) 151, 365
Bme18I GGWCC 2 cut(s) 147, 361
BmgT120I GGNCC 2 cut(s) 147, 361
BmiI GGNNCC 3 cut(s) 148, 149, 331
BmrFI CCNGG 2 cut(s) 151, 365
BmsI GCATC 1 cut(s) 238
BpiI GAAGAC 1 cut(s) 31
BplI GAGNNNNNCTC 2 cut(s) 145, 177
BpuMI CCSGG 1 cut(s) 151
BsaHI GRCGYC 1 cut(s) 330
BsaJI CCNNGG 1 cut(s) 21
BsaWI WCCGGW 1 cut(s) 166
Bsc4I CCNNNNNNNGG 2 cut(s) 144, 370
Bse1I ACTGG 2 cut(s) 217, 394
BseBI CCWGG 1 cut(s) 365
BseDI CCNNGG 1 cut(s) 21
BseGI GGATG 5 cut(s) 42, 194, 200, 253, 398
BseLI CCNNNNNNNGG 2 cut(s) 144, 370
BseMII CTCAG 1 cut(s) 156
BseNI ACTGG 2 cut(s) 217, 394
BseRI GAGGAG 3 cut(s) 87, 97, 100
BseXI GCAGC 1 cut(s) 17
BshFI GGCC 1 cut(s) 29
BshNI GGYRCC 1 cut(s) 329
BsiSI CCGG 2 cut(s) 151, 167
BslFI GGGAC 1 cut(s) 133
BslI CCNNNNNNNGG 2 cut(s) 144, 370
BsmAI GTCTC 1 cut(s) 185
BsmFI GGGAC 1 cut(s) 133
BsnI GGCC 1 cut(s) 29
Bsp19I CCATGG 1 cut(s) 21
BspACI CCGC 4 cut(s) 26, 33, 78, 103
BspANI GGCC 1 cut(s) 29
BspCNI CTCAG 1 cut(s) 155
BspLI GGNNCC 3 cut(s) 148, 149, 331
BspMI ACCTGC 1 cut(s) 77
BspT107I GGYRCC 1 cut(s) 329
BsrBI CCGCTC 1 cut(s) 105
BsrI ACTGG 2 cut(s) 217, 394
BssECI CCNNGG 1 cut(s) 21
BssNI GRCGYC 1 cut(s) 330
BssT1I CCWWGG 1 cut(s) 21
Bst2UI CCWGG 1 cut(s) 365
Bst4CI ACNGT 2 cut(s) 271, 407
Bst6I CTCTTC 2 cut(s) 168, 422
BstACI GRCGYC 1 cut(s) 330
BstDEI CTNAG 1 cut(s) 142
BstDSI CCRYGG 1 cut(s) 21
BstF5I GGATG 5 cut(s) 42, 194, 200, 253, 398
BstH2I RGCGCY 1 cut(s) 333
BstHHI GCGC 1 cut(s) 332
BstMAI GTCTC 1 cut(s) 185
BstMWI GCNNNNNNNGC 3 cut(s) 11, 32, 299
BstNI CCWGG 1 cut(s) 365
BstSCI CCNGG 2 cut(s) 149, 363
BstSFI CTRYAG 2 cut(s) 267, 444
BstV1I GCAGC 1 cut(s) 17
BstV2I GAAGAC 1 cut(s) 31
BsuI GTATCC 1 cut(s) 377
BsuRI GGCC 1 cut(s) 29
BtgI CCRYGG 1 cut(s) 21
BtsCI GGATG 5 cut(s) 42, 194, 200, 253, 398
BtsIMutI CAGTG 4 cut(s) 210, 348, 387, 458
BveI ACCTGC 1 cut(s) 77
CfoI GCGC 1 cut(s) 332
Cfr13I GGNCC 2 cut(s) 147, 361
Csp6I GTAC 1 cut(s) 272
CviAII CATG 1 cut(s) 22
CviJI RGCY 6 cut(s) 14, 29, 157, 176, 379, 443
CviKI_1 RGCY 6 cut(s) 14, 29, 157, 176, 379, 443
CviQI GTAC 1 cut(s) 272
DdeI CTNAG 1 cut(s) 142
DinI GGCGCC 1 cut(s) 331
EaeI YGGCCR 1 cut(s) 27
Eam1104I CTCTTC 2 cut(s) 168, 422
EarI CTCTTC 2 cut(s) 168, 422
EciI GGCGGA 1 cut(s) 67
Eco130I CCWWGG 1 cut(s) 21
Eco47I GGWCC 2 cut(s) 147, 361
Eco57I CTGAAG 1 cut(s) 482
EcoO109I RGGNCCY 2 cut(s) 147, 361
EcoRII CCWGG 1 cut(s) 363
EcoT14I CCWWGG 1 cut(s) 21
EgeI GGCGCC 1 cut(s) 331
EheI GGCGCC 1 cut(s) 331
ErhI CCWWGG 1 cut(s) 21
FaeI CATG 1 cut(s) 25
FaiI YATR 3 cut(s) 23, 280, 282
FaqI GGGAC 1 cut(s) 133
FatI CATG 1 cut(s) 21
FblI GTMKAC 3 cut(s) 207, 266, 402
Fnu4HI GCNGC 3 cut(s) 6, 27, 103
FokI GGATG 5 cut(s) 29, 181, 187, 260, 405
Fsp4HI GCNGC 3 cut(s) 6, 27, 103
FspBI CTAG 2 cut(s) 380, 440
GlaI GCGC 1 cut(s) 331
GluI GCNGC 3 cut(s) 6, 27, 103
HaeII RGCGCY 1 cut(s) 333
HaeIII GGCC 1 cut(s) 29
HapII CCGG 2 cut(s) 151, 167
HhaI GCGC 1 cut(s) 332
Hin1I GRCGYC 1 cut(s) 330
Hin1II CATG 1 cut(s) 25
Hin6I GCGC 1 cut(s) 330
HinP1I GCGC 1 cut(s) 330
HincII GTYRAC 1 cut(s) 208
HindII GTYRAC 1 cut(s) 208
HpaII CCGG 2 cut(s) 151, 167
HphI GGTGA 1 cut(s) 227
Hpy166II GTNNAC 4 cut(s) 208, 218, 267, 403
Hpy188I TCNGA 3 cut(s) 173, 187, 229
Hpy8I GTNNAC 4 cut(s) 208, 218, 267, 403
Hpy99I CGWCG 2 cut(s) 191, 209
HpyAV CCTTC 4 cut(s) 126, 133, 148, 229
HpyCH4III ACNGT 2 cut(s) 271, 407
HpyCH4IV ACGT 1 cut(s) 49
HpyCH4V TGCA 1 cut(s) 251
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 32, 299
HpyF3I CTNAG 1 cut(s) 142
HpySE526I ACGT 1 cut(s) 49
Hsp92I GRCGYC 1 cut(s) 330
Hsp92II CATG 1 cut(s) 25
HspAI GCGC 1 cut(s) 330
KasI GGCGCC 1 cut(s) 329
KflI GGGWCCC 1 cut(s) 147
LmnI GCTCC 1 cut(s) 110
LpnPI CCDG 9 cut(s) 82, 129, 164, 180, 198, 350, 375, 377, 462
Lsp1109I GCAGC 1 cut(s) 17
LweI GCATC 1 cut(s) 238
MaeI CTAG 2 cut(s) 380, 440
MaeII ACGT 1 cut(s) 49
MaeIII GTNAC 1 cut(s) 407
MbiI CCGCTC 1 cut(s) 105
MboII GAAGA 7 cut(s) 31, 111, 155, 174, 344, 409, 475
MluCI AATT 2 cut(s) 337, 368
Mly113I GGCGCC 1 cut(s) 330
MmeI TCCRAC 1 cut(s) 210
MseI TTAA 1 cut(s) 132
MspI CCGG 2 cut(s) 151, 167
MspR9I CCNGG 2 cut(s) 151, 365
MvaI CCWGG 1 cut(s) 365
MwoI GCNNNNNNNGC 3 cut(s) 11, 32, 299
NarI GGCGCC 1 cut(s) 330
NciI CCSGG 1 cut(s) 151
NcoI CCATGG 1 cut(s) 21
NlaIII CATG 1 cut(s) 25
NlaIV GGNNCC 3 cut(s) 148, 149, 331
PaqCI CACCTGC 1 cut(s) 77
PfoI TCCNGGA 2 cut(s) 149, 363
PkrI GCNGC 3 cut(s) 7, 28, 104
PluTI GGCGCC 1 cut(s) 333
PpuMI RGGWCCY 2 cut(s) 147, 361
Psp5II RGGWCCY 2 cut(s) 147, 361
Psp6I CCWGG 1 cut(s) 363
PspGI CCWGG 1 cut(s) 363
PspN4I GGNNCC 3 cut(s) 148, 149, 331
PspPI GGNCC 2 cut(s) 147, 361
PspPPI RGGWCCY 2 cut(s) 147, 361
RsaI GTAC 1 cut(s) 273
RsaNI GTAC 1 cut(s) 272
SalI GTCGAC 1 cut(s) 206
SaqAI TTAA 1 cut(s) 132
SatI GCNGC 3 cut(s) 6, 27, 103
Sau96I GGNCC 2 cut(s) 147, 361
ScaI AGTACT 1 cut(s) 273
ScrFI CCNGG 2 cut(s) 151, 365
SetI ASST 8 cut(s) 52, 71, 93, 178, 289, 363, 414, 445
SfaNI GCATC 1 cut(s) 238
SfcI CTRYAG 2 cut(s) 267, 444
SfoI GGCGCC 1 cut(s) 331
SinI GGWCC 2 cut(s) 147, 361
Sse9I AATT 2 cut(s) 337, 368
SsiI CCGC 4 cut(s) 26, 33, 78, 103
SspDI GGCGCC 1 cut(s) 329
SspMI CTAG 2 cut(s) 380, 440
StyD4I CCNGG 2 cut(s) 149, 363
StyI CCWWGG 1 cut(s) 21
TaaI ACNGT 2 cut(s) 271, 407
TaiI ACGT 1 cut(s) 52
TaqI TCGA 1 cut(s) 207
TasI AATT 2 cut(s) 337, 368
TatI WGTACW 1 cut(s) 271
TauI GCSGC 2 cut(s) 29, 105
Tru1I TTAA 1 cut(s) 132
Tru9I TTAA 1 cut(s) 132
TscAI CASTG 4 cut(s) 217, 348, 394, 465
TseI GCWGC 1 cut(s) 5
TspDTI ATGAA 2 cut(s) 31, 168
TspGWI ACGGA 1 cut(s) 193
TspRI CASTG 4 cut(s) 217, 348, 394, 465
VpaK11BI GGWCC 2 cut(s) 147, 361
XmiI GTMKAC 3 cut(s) 207, 266, 402
XspI CTAG 2 cut(s) 380, 440
ZrmI AGTACT 1 cut(s) 273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.