RchiOBHm_Chr1g0358531

Protein CURVATURE THYLAKOID 1A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0005869
Physical Location & Seq
Reverse (-)
50669803 .. 50672217
2415 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ58366

Sequence Viewer

Length: 486 bp
ATGGCAGCGACAGCCTCCTCCATGGCGGCCACCGCCGTGTTTGTGCCACGTGTCCCCTCCACAACCGCCCGCTGCTCGGCCTTGCGTTACCTCCCACCTCGTGTCTCCTCCTCCCCGTCTTGCTTTGCCATTAGAGCTTCCTCAGAGTCCCGGAGGCGTTCCCTTCAGGTCAGAGCATCATCTGAAGAGACATCCACTTCCGTTGACACTGGAGAACTAATATCAGACTTGAAGGAGAAGTGGGATGCAGTTGAAAACAAGTCAACAGTACTTGTGTATGGAGGTGGGGCAATCGTTGCTTTGTGGCTGTCTTCAATAGTTGTTGGTGCCGTCAATTCAGTGCCACTGCTTCCAAAGATCCTGGAGTTGGTAGGGCTAGGATATACTGGATGGTTCGTCTACCGTTACCTGCTCTTCAAGTCGAGCAGAAAAGAACTAGCTACAGACATTGATGCATTGAAGAAGAAGATCACTGGAACCGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CAAD

Protein Analysis

161

Amino Acids

17.33

Weight (kDa)

9.51

Isoelectric Point (pI)

47.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAAD PF14159 76 - 159 2.6e-32 CAAD domains of cyanobacterial aminoacyl-tRNA synthetase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 417
AccB1I GGYRCC 1 cut(s) 326
AccI GTMKAC 1 cut(s) 399
AciI CCGC 4 cut(s) 26, 33, 66, 70
AclWI GGATC 1 cut(s) 352
AcoI YGGCCR 1 cut(s) 27
AcuI CTGAAG 2 cut(s) 149, 204
AcvI CACGTG 1 cut(s) 50
AdeI CACNNNGTG 1 cut(s) 101
AfaI GTAC 1 cut(s) 270
AfiI CCNNNNNNNGG 2 cut(s) 76, 367
AflIII ACRYGT 1 cut(s) 49
AgsI TTSAA 5 cut(s) 232, 254, 315, 418, 460
AjnI CCWGG 1 cut(s) 360
AleI CACNNNNGTG 1 cut(s) 35
AluBI AGCT 2 cut(s) 137, 440
AluI AGCT 2 cut(s) 137, 440
Alw26I GTCTC 2 cut(s) 109, 182
AlwI GGATC 1 cut(s) 352
AoxI GGCC 2 cut(s) 27, 78
ApeKI GCWGC 2 cut(s) 5, 72
AsuC2I CCSGG 1 cut(s) 151
BanI GGYRCC 1 cut(s) 326
BauI CACGAG 1 cut(s) 99
BbrPI CACGTG 1 cut(s) 50
BbsI GAAGAC 1 cut(s) 303
BbvI GCAGC 2 cut(s) 17, 59
BccI CCATC 1 cut(s) 384
BceAI ACGGC 2 cut(s) 20, 314
BciT130I CCWGG 1 cut(s) 362
BcnI CCSGG 1 cut(s) 151
BcoDI GTCTC 2 cut(s) 109, 182
BfaI CTAG 2 cut(s) 377, 437
BfmI CTRYAG 1 cut(s) 441
BfuAI ACCTGC 1 cut(s) 417
BisI GCNGC 3 cut(s) 6, 27, 73
BlsI GCNGC 3 cut(s) 7, 28, 74
BmcAI AGTACT 1 cut(s) 270
Bme1390I CCNGG 2 cut(s) 151, 362
BmiI GGNNCC 2 cut(s) 328, 478
BmrFI CCNGG 2 cut(s) 151, 362
BmsI GCATC 3 cut(s) 185, 235, 442
BpiI GAAGAC 1 cut(s) 303
BpmI CTGGAG 2 cut(s) 231, 383
BpuMI CCSGG 1 cut(s) 151
BsaAI YACGTR 1 cut(s) 50
BsaJI CCNNGG 1 cut(s) 21
Bsc4I CCNNNNNNNGG 2 cut(s) 76, 367
Bse1I ACTGG 3 cut(s) 214, 391, 478
BseBI CCWGG 1 cut(s) 362
BseDI CCNNGG 1 cut(s) 21
BseGI GGATG 3 cut(s) 191, 250, 395
BseLI CCNNNNNNNGG 2 cut(s) 76, 367
BseMII CTCAG 1 cut(s) 156
BseNI ACTGG 3 cut(s) 214, 391, 478
BseRI GAGGAG 3 cut(s) 7, 97, 100
BseXI GCAGC 2 cut(s) 17, 59
BshFI GGCC 2 cut(s) 29, 80
BshNI GGYRCC 1 cut(s) 326
BsiSI CCGG 1 cut(s) 151
BslFI GGGAC 2 cut(s) 38, 133
BslI CCNNNNNNNGG 2 cut(s) 76, 367
BsmAI GTCTC 2 cut(s) 109, 182
BsmFI GGGAC 2 cut(s) 38, 133
BsnI GGCC 2 cut(s) 29, 80
Bsp143I GATC 2 cut(s) 357, 468
Bsp19I CCATGG 1 cut(s) 21
BspACI CCGC 4 cut(s) 26, 33, 66, 70
BspANI GGCC 2 cut(s) 29, 80
BspCNI CTCAG 1 cut(s) 155
BspLI GGNNCC 2 cut(s) 328, 478
BspMI ACCTGC 1 cut(s) 417
BspPI GGATC 1 cut(s) 352
BspQI GCTCTTC 1 cut(s) 419
BspT107I GGYRCC 1 cut(s) 326
BsrI ACTGG 3 cut(s) 214, 391, 478
BssECI CCNNGG 1 cut(s) 21
BssMI GATC 2 cut(s) 357, 468
BssSI CACGAG 1 cut(s) 99
BssT1I CCWWGG 1 cut(s) 21
Bst2BI CACGAG 1 cut(s) 99
Bst2UI CCWGG 1 cut(s) 362
Bst4CI ACNGT 2 cut(s) 268, 404
Bst6I CTCTTC 2 cut(s) 180, 419
BstAPI GCANNNNNTGC 1 cut(s) 296
BstBAI YACGTR 1 cut(s) 50
BstC8I GCNNGC 1 cut(s) 70
BstDEI CTNAG 1 cut(s) 142
BstDSI CCRYGG 1 cut(s) 21
BstF5I GGATG 3 cut(s) 191, 250, 395
BstKTI GATC 2 cut(s) 360, 471
BstMAI GTCTC 2 cut(s) 109, 182
BstMBI GATC 2 cut(s) 357, 468
BstMWI GCNNNNNNNGC 4 cut(s) 11, 32, 134, 296
BstNI CCWGG 1 cut(s) 362
BstSCI CCNGG 2 cut(s) 149, 360
BstSFI CTRYAG 1 cut(s) 441
BstV1I GCAGC 2 cut(s) 17, 59
BstV2I GAAGAC 1 cut(s) 303
BstX2I RGATCY 1 cut(s) 357
BstYI RGATCY 1 cut(s) 357
BsuRI GGCC 2 cut(s) 29, 80
BtgI CCRYGG 1 cut(s) 21
BtsCI GGATG 3 cut(s) 191, 250, 395
BtsI GCAGTG 1 cut(s) 344
BtsIMutI CAGTG 4 cut(s) 207, 344, 345, 471
BveI ACCTGC 1 cut(s) 417
Cac8I GCNNGC 1 cut(s) 70
Csp6I GTAC 1 cut(s) 269
CspCI CAANNNNNGTGG 2 cut(s) 184, 219
CviAII CATG 1 cut(s) 22
CviJI RGCY 7 cut(s) 14, 29, 80, 137, 307, 376, 440
CviKI_1 RGCY 7 cut(s) 14, 29, 80, 137, 307, 376, 440
CviQI GTAC 1 cut(s) 269
DdeI CTNAG 1 cut(s) 142
DpnI GATC 2 cut(s) 359, 470
DpnII GATC 2 cut(s) 357, 468
DraIII CACNNNGTG 1 cut(s) 101
EaeI YGGCCR 1 cut(s) 27
Eam1104I CTCTTC 2 cut(s) 180, 419
EarI CTCTTC 2 cut(s) 180, 419
Eco130I CCWWGG 1 cut(s) 21
Eco57I CTGAAG 2 cut(s) 149, 204
Eco72I CACGTG 1 cut(s) 50
EcoRII CCWGG 1 cut(s) 360
EcoT14I CCWWGG 1 cut(s) 21
EcoT22I ATGCAT 1 cut(s) 457
ErhI CCWWGG 1 cut(s) 21
FaeI CATG 1 cut(s) 25
FaiI YATR 3 cut(s) 23, 279, 384
FaqI GGGAC 2 cut(s) 38, 133
FatI CATG 1 cut(s) 21
FauI CCCGC 1 cut(s) 77
FblI GTMKAC 1 cut(s) 399
Fnu4HI GCNGC 3 cut(s) 6, 27, 73
FokI GGATG 3 cut(s) 178, 257, 402
Fsp4HI GCNGC 3 cut(s) 6, 27, 73
FspBI CTAG 2 cut(s) 377, 437
GluI GCNGC 3 cut(s) 6, 27, 73
GsuI CTGGAG 2 cut(s) 231, 383
HaeIII GGCC 2 cut(s) 29, 80
HapII CCGG 1 cut(s) 151
Hin1II CATG 1 cut(s) 25
HincII GTYRAC 2 cut(s) 205, 264
HindII GTYRAC 2 cut(s) 205, 264
HinfI GANTC 1 cut(s) 146
HpaII CCGG 1 cut(s) 151
Hpy166II GTNNAC 3 cut(s) 205, 264, 400
Hpy188I TCNGA 4 cut(s) 145, 173, 184, 226
Hpy8I GTNNAC 3 cut(s) 205, 264, 400
HpyAV CCTTC 2 cut(s) 173, 226
HpyCH4III ACNGT 2 cut(s) 268, 404
HpyCH4IV ACGT 1 cut(s) 49
HpyCH4V TGCA 2 cut(s) 248, 455
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 32, 134, 296
HpyF3I CTNAG 1 cut(s) 142
HpySE526I ACGT 1 cut(s) 49
Hsp92II CATG 1 cut(s) 25
Kzo9I GATC 2 cut(s) 357, 468
LguI GCTCTTC 1 cut(s) 419
LpnPI CCDG 8 cut(s) 152, 164, 195, 347, 372, 374, 422, 459
Lsp1109I GCAGC 2 cut(s) 17, 59
LweI GCATC 3 cut(s) 185, 235, 442
MaeI CTAG 2 cut(s) 377, 437
MaeII ACGT 1 cut(s) 49
MaeIII GTNAC 2 cut(s) 86, 404
MalI GATC 2 cut(s) 359, 470
MboI GATC 2 cut(s) 357, 468
MboII GAAGA 6 cut(s) 197, 303, 406, 472, 475, 478
MflI RGATCY 1 cut(s) 357
MluCI AATT 1 cut(s) 334
MlyI GAGTC 1 cut(s) 155
Mph1103I ATGCAT 1 cut(s) 457
MslI CAYNNNNRTG 1 cut(s) 35
MspA1I CMGCKG 1 cut(s) 72
MspI CCGG 1 cut(s) 151
MspR9I CCNGG 2 cut(s) 151, 362
MvaI CCWGG 1 cut(s) 362
MwoI GCNNNNNNNGC 4 cut(s) 11, 32, 134, 296
NciI CCSGG 1 cut(s) 151
NcoI CCATGG 1 cut(s) 21
NdeII GATC 2 cut(s) 357, 468
NlaIII CATG 1 cut(s) 25
NlaIV GGNNCC 2 cut(s) 328, 478
NmeAIII GCCGAG 1 cut(s) 56
NsiI ATGCAT 1 cut(s) 457
OliI CACNNNNGTG 1 cut(s) 35
PciSI GCTCTTC 1 cut(s) 419
PfoI TCCNGGA 2 cut(s) 149, 360
PkrI GCNGC 3 cut(s) 7, 28, 74
PleI GAGTC 1 cut(s) 154
PmaCI CACGTG 1 cut(s) 50
PmlI CACGTG 1 cut(s) 50
PpsI GAGTC 1 cut(s) 154
Ppu21I YACGTR 1 cut(s) 50
Psp6I CCWGG 1 cut(s) 360
PspCI CACGTG 1 cut(s) 50
PspGI CCWGG 1 cut(s) 360
PspN4I GGNNCC 2 cut(s) 328, 478
PsuI RGATCY 1 cut(s) 357
RsaI GTAC 1 cut(s) 270
RsaNI GTAC 1 cut(s) 269
RseI CAYNNNNRTG 1 cut(s) 35
SapI GCTCTTC 1 cut(s) 419
SatI GCNGC 3 cut(s) 6, 27, 73
Sau3AI GATC 2 cut(s) 357, 468
ScaI AGTACT 1 cut(s) 270
SchI GAGTC 1 cut(s) 155
ScrFI CCNGG 2 cut(s) 151, 362
SetI ASST 8 cut(s) 52, 93, 100, 139, 171, 286, 411, 442
SfaNI GCATC 3 cut(s) 185, 235, 442
SfcI CTRYAG 1 cut(s) 441
SmiMI CAYNNNNRTG 1 cut(s) 35
Sse9I AATT 1 cut(s) 334
SsiI CCGC 4 cut(s) 26, 33, 66, 70
SspMI CTAG 2 cut(s) 377, 437
StyD4I CCNGG 2 cut(s) 149, 360
StyI CCWWGG 1 cut(s) 21
TaaI ACNGT 2 cut(s) 268, 404
TaiI ACGT 1 cut(s) 52
TaqI TCGA 1 cut(s) 422
TasI AATT 1 cut(s) 334
TatI WGTACW 1 cut(s) 268
TauI GCSGC 1 cut(s) 29
TscAI CASTG 4 cut(s) 214, 345, 351, 478
TseI GCWGC 2 cut(s) 5, 72
TspGWI ACGGA 1 cut(s) 190
TspRI CASTG 4 cut(s) 214, 345, 351, 478
XmiI GTMKAC 1 cut(s) 399
XspI CTAG 2 cut(s) 377, 437
ZrmI AGTACT 1 cut(s) 270
Zsp2I ATGCAT 1 cut(s) 457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.