MD07G1225200.v1.1
MYB Family

Myb-like DNA-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0012871
Physical Location & Seq
Reverse (-)
30171011 .. 30172813
1803 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1225200.v1.1.491

Sequence Viewer

Length: 1119 bp
ATGAAGAGTGAAAGCACCAGTATTGAAGAAGTTTCTAAATCAAGCCCTTCTACCAGCAAGAAGGAATACGATCACGAAGACGATGAGGGTGAAGAGGATGAGGTGGATGAAGAAGGCCTTCAGCTGAGGAATAACGGTGGCAGCTCAAGCAATAGCACCATTGAAGAGAATCATGAAAAGAAGGGAGCTTCTGGATCTGTAAGGCAGTATGTTCGTTCCAAGACATCGCGGCTTCGATGGACACCTGATCTCCATCTTTGCTTTATTCGTGCAGTTGAAAGATTAGGTGGACAAGAAAGAGCAACTCCTAAGTTGGTTCTTCAATTGATGAATGTCAAGGGTCTTAGCATAGCTCATGTCAAGAGCCATCTTCAAATGTATAGGAGCAAGAAGACGGAAGACCACAATCAAGTTGTGACGGATCAAGCAGGATTTTTCATGGAAGGTGGAGATAACCAACTTTACAACCTTACTCAACTCTCCATGCTACAAAGCTTCAACCGATGGCCTTCTTCCAGATTAAGATATGGTGCTAATGATCATTCCTCGTGGCGAGGCCGCCACCACCAGATTTACAGTCCTTACAGTTCAAGTAGGACTACTGCACTACTTGATCATAATACCAGAAATATTGGACTTTATGGTTCAGTTGCTGAAAGAATACTTGGAAGCAACAATACCAACATTATGAGTACTTCAGCAAATCAGTTATATACAAATCTTCCATCTCCTAGCCATGGACAAAGGAATCAAATTCCTTGGGACGAGTTGTGTCAAACCATGATCCACAGGCCATGCCAAGATCATCATAGTTGGACCGCAGTGATTCGAGACCATCAAGGGCAAAACATGCTGAAAAGGAAGGGTTTGGACACAGACAACTGTGACTTAGATTTAAATCTGTCACTAAAAGTTCCACCAAAACATGATCATGGATTCGGAAAGGGTTTACAGTGTGGTGATGATAATCATCAAAAACTACTCATGGGTTGTAGTTTATCTCTCACTTTGTCTTCGTCGTCGTCCTCGAAGCTTGGCGTCAAGAAACTAGAGGGTACTGGTTACGGTGACGGCAAGCACGGGAAGAACATGGCAAGTACTCTGGACCTCACTTTATGA

Protein Analysis

373

Amino Acids

41.63

Weight (kDa)

7.32

Isoelectric Point (pI)

45.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 77 - 128 1.6e-08 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012871)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40260 AT2G40260
fragaria_vesca FvH4_7g24880
malus_domestica MD01G1156500.v1.1 MD07G1225200.v1.1
prunus_persica Prupe.2G253700_v2.0.a1
pyrus_communis pycom01g17330
rosa_chinensis RchiOBHm_Chr1g0370761
rosa_laevigata RLG00000027012
rosa_multiflora Rmu_sc0009474.1_g000002 Rmu_sc0009615.1_g000002
rosa_roxburghii Rroxscaffold_4G00286260
rosa_rugosa Rorug01G0359400
rosa_samantha Rh1AG368600 Rh1BG332200 Rh1CG346100 Rh1DG363600
rosa_wichuraiana Rw1G032450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 229
AciI CCGC 3 cut(s) 229, 559, 819
AclWI GGATC 3 cut(s) 202, 429, 778
AcsI RAATTY 1 cut(s) 753
AcuI CTGAAG 2 cut(s) 104, 681
AcyI GRCGYC 1 cut(s) 1038
AfaI GTAC 3 cut(s) 694, 1057, 1099
AfiI CCNNNNNNNGG 1 cut(s) 737
AgsI TTSAA 7 cut(s) 26, 164, 278, 323, 374, 499, 591
AjuI GAANNNNNNNTTGG 2 cut(s) 648, 680
AluBI AGCT 6 cut(s) 124, 144, 188, 353, 495, 1033
AluI AGCT 6 cut(s) 124, 144, 188, 353, 495, 1033
Alw26I GTCTC 1 cut(s) 825
AlwI GGATC 3 cut(s) 202, 429, 778
AlwNI CAGNNNCTG 1 cut(s) 653
AoxI GGCC 4 cut(s) 115, 506, 556, 791
ApeKI GCWGC 1 cut(s) 141
ApoI RAATTY 1 cut(s) 753
Asp700I GAANNNNTTC 1 cut(s) 117
AspS9I GGNCC 2 cut(s) 816, 1105
AsuHPI GGTGA 3 cut(s) 101, 971, 1079
AvaII GGWCC 2 cut(s) 816, 1105
BarI GAAGNNNNNNTAC 2 cut(s) 661, 693
BauI CACGAG 1 cut(s) 547
BbsI GAAGAC 4 cut(s) 84, 398, 405, 1005
BbvCI CCTCAGC 1 cut(s) 125
BbvI GCAGC 1 cut(s) 153
BccI CCATC 6 cut(s) 231, 261, 375, 498, 733, 843
BceAI ACGGC 1 cut(s) 1087
BcgI CGANNNNNNTGC 2 cut(s) 194, 228
BclI TGATCA 3 cut(s) 538, 613, 928
BcoDI GTCTC 1 cut(s) 825
BfaI CTAG 2 cut(s) 732, 1049
BisI GCNGC 3 cut(s) 142, 230, 559
BlsI GCNGC 3 cut(s) 143, 231, 560
BmcAI AGTACT 2 cut(s) 694, 1099
Bme18I GGWCC 2 cut(s) 816, 1105
BmgT120I GGNCC 2 cut(s) 816, 1105
BpiI GAAGAC 4 cut(s) 84, 398, 405, 1005
Bpu10I CCTNAGC 1 cut(s) 125
BpuEI CTTGAG 1 cut(s) 130
BsaBI GATNNNNATC 4 cut(s) 252, 897, 966, 969
BsaHI GRCGYC 1 cut(s) 1038
BsaI GGTCTC 1 cut(s) 825
BsaJI CCNNGG 2 cut(s) 736, 758
BsaXI ACNNNNNCTCC 2 cut(s) 234, 264
Bsc4I CCNNNNNNNGG 1 cut(s) 737
Bse1I ACTGG 2 cut(s) 18, 1063
Bse8I GATNNNNATC 4 cut(s) 252, 897, 966, 969
BseDI CCNNGG 2 cut(s) 736, 758
BseGI GGATG 2 cut(s) 103, 112
BseJI GATNNNNATC 4 cut(s) 252, 897, 966, 969
BseLI CCNNNNNNNGG 1 cut(s) 737
BseMII CTCAG 1 cut(s) 116
BseNI ACTGG 2 cut(s) 18, 1063
BseXI GCAGC 1 cut(s) 153
BsgI GTGCAG 2 cut(s) 291, 588
Bsh1236I CGCG 1 cut(s) 229
BshFI GGCC 4 cut(s) 117, 508, 558, 793
BslFI GGGAC 1 cut(s) 776
BslI CCNNNNNNNGG 1 cut(s) 737
BsmAI GTCTC 1 cut(s) 825
BsmFI GGGAC 1 cut(s) 776
BsnI GGCC 4 cut(s) 117, 508, 558, 793
Bso31I GGTCTC 1 cut(s) 825
Bsp143I GATC 9 cut(s) 70, 194, 247, 421, 538, 613, 783, 802, 928
Bsp19I CCATGG 1 cut(s) 736
BspACI CCGC 3 cut(s) 229, 559, 819
BspANI GGCC 4 cut(s) 117, 508, 558, 793
BspCNI CTCAG 1 cut(s) 117
BspFNI CGCG 1 cut(s) 229
BspHI TCATGA 1 cut(s) 172
BspPI GGATC 3 cut(s) 202, 429, 778
BspTNI GGTCTC 1 cut(s) 825
BsrI ACTGG 2 cut(s) 18, 1063
BssECI CCNNGG 2 cut(s) 736, 758
BssMI GATC 9 cut(s) 70, 194, 247, 421, 538, 613, 783, 802, 928
BssNI GRCGYC 1 cut(s) 1038
BssSI CACGAG 1 cut(s) 547
BssT1I CCWWGG 2 cut(s) 736, 758
Bst2BI CACGAG 1 cut(s) 547
Bst4CI ACNGT 6 cut(s) 137, 578, 587, 884, 954, 1067
Bst6I CTCTTC 2 cut(s) 87, 159
BstACI GRCGYC 1 cut(s) 1038
BstAPI GCANNNNNTGC 1 cut(s) 850
BstC8I GCNNGC 1 cut(s) 1076
BstDEI CTNAG 4 cut(s) 125, 309, 344, 889
BstDSI CCRYGG 1 cut(s) 736
BstF5I GGATG 2 cut(s) 103, 112
BstFNI CGCG 1 cut(s) 229
BstKTI GATC 9 cut(s) 73, 197, 250, 424, 541, 616, 786, 805, 931
BstMAI GTCTC 1 cut(s) 825
BstMBI GATC 9 cut(s) 70, 194, 247, 421, 538, 613, 783, 802, 928
BstMWI GCNNNNNNNGC 2 cut(s) 147, 850
BstNSI RCATGY 1 cut(s) 853
BstUI CGCG 1 cut(s) 229
BstV1I GCAGC 1 cut(s) 153
BstV2I GAAGAC 4 cut(s) 84, 398, 405, 1005
BstX2I RGATCY 1 cut(s) 194
BstYI RGATCY 1 cut(s) 194
BsuRI GGCC 4 cut(s) 117, 508, 558, 793
BtgI CCRYGG 1 cut(s) 736
BtgZI GCGATG 1 cut(s) 210
BtsCI GGATG 2 cut(s) 103, 112
BtsI GCAGTG 1 cut(s) 828
BtsIMutI CAGTG 2 cut(s) 828, 959
Cac8I GCNNGC 1 cut(s) 1076
CaiI CAGNNNCTG 1 cut(s) 653
CciI TCATGA 1 cut(s) 172
Cfr13I GGNCC 2 cut(s) 816, 1105
CseI GACGC 1 cut(s) 1027
Csp6I GTAC 3 cut(s) 693, 1056, 1098
CviQI GTAC 3 cut(s) 693, 1056, 1098
DdeI CTNAG 4 cut(s) 125, 309, 344, 889
DpnI GATC 9 cut(s) 72, 196, 249, 423, 540, 615, 785, 804, 930
DpnII GATC 9 cut(s) 70, 194, 247, 421, 538, 613, 783, 802, 928
DraI TTTAAA 1 cut(s) 897
Eam1104I CTCTTC 2 cut(s) 87, 159
EarI CTCTTC 2 cut(s) 87, 159
Eco130I CCWWGG 2 cut(s) 736, 758
Eco147I AGGCCT 1 cut(s) 117
Eco31I GGTCTC 1 cut(s) 825
Eco47I GGWCC 2 cut(s) 816, 1105
Eco57I CTGAAG 2 cut(s) 104, 681
EcoT14I CCWWGG 2 cut(s) 736, 758
ErhI CCWWGG 2 cut(s) 736, 758
FalI AAGNNNNNCTT 2 cut(s) 102, 134
FaqI GGGAC 1 cut(s) 776
FbaI TGATCA 3 cut(s) 538, 613, 928
Fnu4HI GCNGC 3 cut(s) 142, 230, 559
FokI GGATG 2 cut(s) 110, 119
Fsp4HI GCNGC 3 cut(s) 142, 230, 559
FspBI CTAG 2 cut(s) 732, 1049
GluI GCNGC 3 cut(s) 142, 230, 559
HaeIII GGCC 4 cut(s) 117, 508, 558, 793
HgaI GACGC 1 cut(s) 1027
Hin1I GRCGYC 1 cut(s) 1038
HindIII AAGCTT 2 cut(s) 493, 1031
HinfI GANTC 4 cut(s) 169, 748, 826, 936
HphI GGTGA 3 cut(s) 101, 971, 1079
Hpy166II GTNNAC 2 cut(s) 290, 950
Hpy188I TCNGA 1 cut(s) 941
Hpy188III TCNNGA 8 cut(s) 74, 173, 192, 361, 516, 830, 1042, 1103
Hpy8I GTNNAC 2 cut(s) 290, 950
Hpy99I CGWCG 2 cut(s) 1021, 1024
HpyAV CCTTC 8 cut(s) 55, 57, 107, 128, 175, 437, 519, 856
HpyCH4III ACNGT 6 cut(s) 137, 578, 587, 884, 954, 1067
HpyCH4V TGCA 2 cut(s) 272, 605
HpyF10VI GCNNNNNNNGC 2 cut(s) 147, 850
HpyF3I CTNAG 4 cut(s) 125, 309, 344, 889
Hsp92I GRCGYC 1 cut(s) 1038
Ksp22I TGATCA 3 cut(s) 538, 613, 928
Kzo9I GATC 9 cut(s) 70, 194, 247, 421, 538, 613, 783, 802, 928
LmnI GCTCC 2 cut(s) 185, 384
Lsp1109I GCAGC 1 cut(s) 153
MaeI CTAG 2 cut(s) 732, 1049
MaeIII GTNAC 5 cut(s) 415, 884, 903, 1061, 1067
MalI GATC 9 cut(s) 72, 196, 249, 423, 540, 615, 785, 804, 930
MboI GATC 9 cut(s) 70, 194, 247, 421, 538, 613, 783, 802, 928
MfeI CAATTG 1 cut(s) 323
MflI RGATCY 1 cut(s) 194
MluCI AATT 2 cut(s) 323, 753
MmeI TCCRAC 1 cut(s) 794
MnlI CCTC 9 cut(s) 79, 88, 94, 120, 548, 556, 1036, 1045, 1118
MroXI GAANNNNTTC 1 cut(s) 117
MseI TTAA 2 cut(s) 521, 896
MslI CAYNNNNRTG 1 cut(s) 930
MspA1I CMGCKG 1 cut(s) 124
MunI CAATTG 1 cut(s) 323
MvnI CGCG 1 cut(s) 229
MwoI GCNNNNNNNGC 2 cut(s) 147, 850
NcoI CCATGG 1 cut(s) 736
NdeII GATC 9 cut(s) 70, 194, 247, 421, 538, 613, 783, 802, 928
NmuCI GTSAC 4 cut(s) 415, 884, 903, 1067
NspI RCATGY 1 cut(s) 853
PagI TCATGA 1 cut(s) 172
PceI AGGCCT 1 cut(s) 117
PcsI WCGNNNNNNNCGW 1 cut(s) 1025
PdmI GAANNNNTTC 1 cut(s) 117
PfeI GAWTC 4 cut(s) 169, 748, 826, 936
PkrI GCNGC 3 cut(s) 143, 231, 560
PspPI GGNCC 2 cut(s) 816, 1105
PstNI CAGNNNCTG 1 cut(s) 653
PsuI RGATCY 1 cut(s) 194
PvuII CAGCTG 1 cut(s) 124
RsaI GTAC 3 cut(s) 694, 1057, 1099
RsaNI GTAC 3 cut(s) 693, 1056, 1098
RseI CAYNNNNRTG 1 cut(s) 930
SaqAI TTAA 2 cut(s) 521, 896
SatI GCNGC 3 cut(s) 142, 230, 559
Sau3AI GATC 9 cut(s) 70, 194, 247, 421, 538, 613, 783, 802, 928
Sau96I GGNCC 2 cut(s) 816, 1105
ScaI AGTACT 2 cut(s) 694, 1099
SinI GGWCC 2 cut(s) 816, 1105
SmiI ATTTAAAT 1 cut(s) 897
SmiMI CAYNNNNRTG 1 cut(s) 930
SmlI CTYRAG 1 cut(s) 145
SmoI CTYRAG 1 cut(s) 145
Sse9I AATT 2 cut(s) 323, 753
SseBI AGGCCT 1 cut(s) 117
SsiI CCGC 3 cut(s) 229, 559, 819
SspI AATATT 1 cut(s) 631
SspMI CTAG 2 cut(s) 732, 1049
StuI AGGCCT 1 cut(s) 117
StyI CCWWGG 2 cut(s) 736, 758
SwaI ATTTAAAT 1 cut(s) 897
TaaI ACNGT 6 cut(s) 137, 578, 587, 884, 954, 1067
TaqI TCGA 3 cut(s) 235, 829, 1028
TasI AATT 2 cut(s) 323, 753
TatI WGTACW 2 cut(s) 692, 1097
TauI GCSGC 2 cut(s) 232, 561
TfiI GAWTC 4 cut(s) 169, 748, 826, 936
Tru1I TTAA 2 cut(s) 521, 896
Tru9I TTAA 2 cut(s) 521, 896
TscAI CASTG 2 cut(s) 828, 959
TseFI GTSAC 4 cut(s) 415, 884, 903, 1067
TseI GCWGC 1 cut(s) 141
Tsp45I GTSAC 4 cut(s) 415, 884, 903, 1067
TspDTI ATGAA 5 cut(s) 17, 123, 189, 344, 427
TspGWI ACGGA 2 cut(s) 410, 434
TspRI CASTG 2 cut(s) 828, 959
VpaK11BI GGWCC 2 cut(s) 816, 1105
XapI RAATTY 1 cut(s) 753
XceI RCATGY 1 cut(s) 853
XmnI GAANNNNTTC 1 cut(s) 117
XspI CTAG 2 cut(s) 732, 1049
ZrmI AGTACT 2 cut(s) 694, 1099
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.