Rroxscaffold_4G00286260
MYB Family

Myb-like DNA-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0012871
Physical Location & Seq
Forward (+)
7353827 .. 7356006
2180 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00286260.1

Sequence Viewer

Length: 1122 bp
ATGAAGATATCAAGTGAAGGTACCAGTCAAGCTTCTAAAGCAAGTTTATCATCTTCTAGCCAGAGCAAGAAGGAACGTGGTGACGATGAGGAGGAGGATGAGGAGGTGGTGGATAAGGATAGCCAGCAACAGCCCAGCAGCAAGAATGGTGTTAGCTCCAGTAGCAGCACCATTGAAGAGAACGAAAAGTCGAAGGGTGCTTCAGATGGATCTGTACGACAGTATGTGCGTTCCAAGACTTTAAGGCTTCGATGGACTCCTGATCTCCATCTTTGCTTTATTCATGCGGTTGAAAGATTAGGAGGGCAAGAGAGAGCAACACCAAAATTAGTTCTTCAATTGATGAATGTCAAGGGTCTCAGCATAGCACATGTCAAGAGCCATCTTCAAATGTATAGGAGCAAGAAGATGGAAGACCCAAATCAAGTTCTCTCAAAGCAAGGCTTCTACATTGAAGGTGGAGATAATCATATATACAACCTTACTCAACTCTCCGTGCTACAAAGTTTCAACCACCAATGGCCTTCTTCAGGATTGAGATATGGTGCTGATGCCCCTTGGTGTGGCCGCCACGACGACCACCACCACCAGATTTATAGCCCTTACAGTAATTCAGGCAGGACTCAACTATTTGATCGTCATAATACAAGAGTTACAGGACTTTATGGTTCAGTTGCTGAGAGAATATTTGGAAGCAACAACACCAGTGCTAGTACAACTACTACTGCTGCTACTACACCGAACGATATTCAATCACATACAACTCATATTCCATCCACTTGGAGATCAAGGCATCAAATTAGTACTCGAGTCCACAACGAGTTCCGACCCTTCAACAGGCAGTCATGGCAAGATCACAGTTGGTTGGACAACAGTATCGCCACTCGAAACACACTGAAAAGAAAGGCTCCGGATTCTTCAGATAATACTTCTAATTTGGATTTGAACTTATCACTGAAAGTTCCAGCAACAGAAGAAAAGGTACTGGGATTTGGTCTGTCTCTTTCTCTTACTCCAGCTTCAGCGTCCTCGAAGCTCCGCTTGATGAAGAAGCTAGAAGGTAATGGTGGTCATGATAGGGAGCAAGGGAGAAAGGCAAGTACTCTGGACCTCACTCTATGA

Protein Analysis

373

Amino Acids

41.61

Weight (kDa)

9.15

Isoelectric Point (pI)

53.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 82 - 133 2.4e-08 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012871)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40260 AT2G40260
fragaria_vesca FvH4_7g24880
malus_domestica MD01G1156500.v1.1 MD07G1225200.v1.1
prunus_persica Prupe.2G253700_v2.0.a1
pyrus_communis pycom01g17330
rosa_chinensis RchiOBHm_Chr1g0370761
rosa_laevigata RLG00000027012
rosa_multiflora Rmu_sc0009474.1_g000002 Rmu_sc0009615.1_g000002
rosa_roxburghii Rroxscaffold_4G00286260
rosa_rugosa Rorug01G0359400
rosa_samantha Rh1AG368600 Rh1BG332200 Rh1CG346100 Rh1DG363600
rosa_wichuraiana Rw1G032450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 20
AccB1I GGYRCC 1 cut(s) 20
AccIII TCCGGA 1 cut(s) 910
AciI CCGC 3 cut(s) 287, 568, 1039
AclWI GGATC 1 cut(s) 217
AcoI YGGCCR 1 cut(s) 565
AcuI CTGAAG 4 cut(s) 186, 513, 903, 1005
AfaI GTAC 6 cut(s) 22, 216, 715, 805, 984, 1102
AfiI CCNNNNNNNGG 3 cut(s) 530, 563, 837
AflIII ACRYGT 1 cut(s) 370
AgsI TTSAA 9 cut(s) 176, 293, 338, 389, 455, 511, 752, 835, 946
AluBI AGCT 5 cut(s) 32, 156, 1019, 1036, 1054
AluI AGCT 5 cut(s) 32, 156, 1019, 1036, 1054
Alw26I GTCTC 2 cut(s) 362, 1005
AlwI GGATC 1 cut(s) 217
AlwNI CAGNNNCTG 1 cut(s) 677
Ama87I CYCGRG 1 cut(s) 807
Aor13HI TCCGGA 1 cut(s) 910
AoxI GGCC 2 cut(s) 521, 565
ApeKI GCWGC 3 cut(s) 138, 165, 728
Asp718I GGTACC 1 cut(s) 20
AspS9I GGNCC 1 cut(s) 1108
AsuHPI GGTGA 1 cut(s) 92
AvaI CYCGRG 1 cut(s) 807
AvaII GGWCC 1 cut(s) 1108
BaeI ACNNNNGTAYC 2 cut(s) 859, 892
BanI GGYRCC 1 cut(s) 20
BarI GAAGNNNNNNTAC 2 cut(s) 966, 998
BbsI GAAGAC 1 cut(s) 420
BbvI GCAGC 3 cut(s) 150, 177, 715
BccI CCATC 6 cut(s) 200, 246, 276, 390, 403, 781
BcoDI GTCTC 2 cut(s) 362, 1005
BfaI CTAG 3 cut(s) 57, 711, 1055
BisI GCNGC 4 cut(s) 139, 166, 568, 729
BlsI GCNGC 4 cut(s) 140, 167, 569, 730
BmcAI AGTACT 2 cut(s) 805, 1102
Bme18I GGWCC 1 cut(s) 1108
BmeT110I CYCGRG 1 cut(s) 807
BmgT120I GGNCC 1 cut(s) 1108
BmiI GGNNCC 2 cut(s) 22, 909
BmrI ACTGGG 1 cut(s) 995
BmsI GCATC 2 cut(s) 541, 802
BmuI ACTGGG 1 cut(s) 995
BpiI GAAGAC 1 cut(s) 420
BpmI CTGGAG 2 cut(s) 142, 999
BsaBI GATNNNNATC 1 cut(s) 267
BsaI GGTCTC 1 cut(s) 362
BsaJI CCNNGG 1 cut(s) 557
BsaWI WCCGGW 1 cut(s) 910
Bsc4I CCNNNNNNNGG 3 cut(s) 530, 563, 837
Bse1I ACTGG 4 cut(s) 24, 159, 705, 990
Bse8I GATNNNNATC 1 cut(s) 267
BseAI TCCGGA 1 cut(s) 910
BseDI CCNNGG 1 cut(s) 557
BseGI GGATG 2 cut(s) 103, 773
BseJI GATNNNNATC 1 cut(s) 267
BseLI CCNNNNNNNGG 3 cut(s) 530, 563, 837
BseMII CTCAG 2 cut(s) 373, 669
BseNI ACTGG 4 cut(s) 24, 159, 705, 990
BseRI GAGGAG 3 cut(s) 104, 107, 116
BseXI GCAGC 3 cut(s) 150, 177, 715
BseYI CCCAGC 1 cut(s) 134
BshFI GGCC 2 cut(s) 523, 567
BshNI GGYRCC 1 cut(s) 20
BsiHKCI CYCGRG 1 cut(s) 807
BsiSI CCGG 1 cut(s) 911
BslI CCNNNNNNNGG 3 cut(s) 530, 563, 837
BsmAI GTCTC 2 cut(s) 362, 1005
BsnI GGCC 2 cut(s) 523, 567
Bso31I GGTCTC 1 cut(s) 362
BsoBI CYCGRG 1 cut(s) 807
Bsp13I TCCGGA 1 cut(s) 910
Bsp143I GATC 5 cut(s) 209, 262, 634, 785, 853
BspACI CCGC 3 cut(s) 287, 568, 1039
BspANI GGCC 2 cut(s) 523, 567
BspCNI CTCAG 2 cut(s) 372, 670
BspEI TCCGGA 1 cut(s) 910
BspHI TCATGA 1 cut(s) 1072
BspLI GGNNCC 2 cut(s) 22, 909
BspPI GGATC 1 cut(s) 217
BspT107I GGYRCC 1 cut(s) 20
BspTNI GGTCTC 1 cut(s) 362
BsrI ACTGG 4 cut(s) 24, 159, 705, 990
BssECI CCNNGG 1 cut(s) 557
BssMI GATC 5 cut(s) 209, 262, 634, 785, 853
BssT1I CCWWGG 1 cut(s) 557
Bst4CI ACNGT 4 cut(s) 222, 608, 860, 875
Bst6I CTCTTC 1 cut(s) 171
BstC8I GCNNGC 1 cut(s) 125
BstDEI CTNAG 2 cut(s) 359, 678
BstENI CCTNNNNNAGG 2 cut(s) 528, 835
BstF5I GGATG 2 cut(s) 103, 773
BstKTI GATC 5 cut(s) 212, 265, 637, 788, 856
BstMAI GTCTC 2 cut(s) 362, 1005
BstMBI GATC 5 cut(s) 209, 262, 634, 785, 853
BstMWI GCNNNNNNNGC 3 cut(s) 38, 162, 847
BstNSI RCATGY 1 cut(s) 374
BstV1I GCAGC 3 cut(s) 150, 177, 715
BstV2I GAAGAC 1 cut(s) 420
BstX2I RGATCY 1 cut(s) 209
BstXI CCANNNNNNTGG 1 cut(s) 780
BstYI RGATCY 1 cut(s) 209
BsuRI GGCC 2 cut(s) 523, 567
BtsCI GGATG 2 cut(s) 103, 773
BtsIMutI CAGTG 3 cut(s) 712, 893, 953
Cac8I GCNNGC 1 cut(s) 125
CaiI CAGNNNCTG 1 cut(s) 677
CciI TCATGA 1 cut(s) 1072
Cfr13I GGNCC 1 cut(s) 1108
CseI GACGC 1 cut(s) 1014
Csp6I GTAC 6 cut(s) 21, 215, 714, 804, 983, 1101
CviAII CATG 4 cut(s) 284, 371, 846, 1073
CviQI GTAC 6 cut(s) 21, 215, 714, 804, 983, 1101
DdeI CTNAG 2 cut(s) 359, 678
DpnI GATC 5 cut(s) 211, 264, 636, 787, 855
DpnII GATC 5 cut(s) 209, 262, 634, 785, 853
EaeI YGGCCR 1 cut(s) 565
Eam1104I CTCTTC 1 cut(s) 171
EarI CTCTTC 1 cut(s) 171
Eco130I CCWWGG 1 cut(s) 557
Eco31I GGTCTC 1 cut(s) 362
Eco32I GATATC 1 cut(s) 9
Eco47I GGWCC 1 cut(s) 1108
Eco57I CTGAAG 4 cut(s) 186, 513, 903, 1005
Eco88I CYCGRG 1 cut(s) 807
EcoNI CCTNNNNNAGG 2 cut(s) 528, 835
EcoRV GATATC 1 cut(s) 9
EcoT14I CCWWGG 1 cut(s) 557
ErhI CCWWGG 1 cut(s) 557
FaeI CATG 4 cut(s) 287, 374, 849, 1076
FalI AAGNNNNNCTT 4 cut(s) 428, 460, 1025, 1057
FatI CATG 4 cut(s) 283, 370, 845, 1072
Fnu4HI GCNGC 4 cut(s) 139, 166, 568, 729
FokI GGATG 2 cut(s) 110, 760
Fsp4HI GCNGC 4 cut(s) 139, 166, 568, 729
FspBI CTAG 3 cut(s) 57, 711, 1055
GluI GCNGC 4 cut(s) 139, 166, 568, 729
GsaI CCCAGC 1 cut(s) 138
GsuI CTGGAG 2 cut(s) 142, 999
HaeIII GGCC 2 cut(s) 523, 567
HapII CCGG 1 cut(s) 911
HgaI GACGC 1 cut(s) 1014
Hin1II CATG 4 cut(s) 287, 374, 849, 1076
HindIII AAGCTT 1 cut(s) 30
HinfI GANTC 4 cut(s) 256, 622, 810, 914
HpaII CCGG 1 cut(s) 911
HphI GGTGA 1 cut(s) 92
Hpy166II GTNNAC 1 cut(s) 814
Hpy188I TCNGA 3 cut(s) 205, 827, 922
Hpy188III TCNNGA 6 cut(s) 260, 376, 531, 911, 1073, 1106
Hpy8I GTNNAC 1 cut(s) 814
Hpy99I CGWCG 1 cut(s) 578
HpyAV CCTTC 7 cut(s) 11, 64, 187, 449, 534, 841, 1052
HpyCH4III ACNGT 4 cut(s) 222, 608, 860, 875
HpyCH4IV ACGT 1 cut(s) 76
HpyF10VI GCNNNNNNNGC 3 cut(s) 38, 162, 847
HpyF3I CTNAG 2 cut(s) 359, 678
HpySE526I ACGT 1 cut(s) 76
Hsp92II CATG 4 cut(s) 287, 374, 849, 1076
Kpn2I TCCGGA 1 cut(s) 910
KpnI GGTACC 1 cut(s) 24
Kzo9I GATC 5 cut(s) 209, 262, 634, 785, 853
LmnI GCTCC 5 cut(s) 161, 399, 913, 1041, 1081
Lsp1109I GCAGC 3 cut(s) 150, 177, 715
LweI GCATC 2 cut(s) 541, 802
MaeI CTAG 3 cut(s) 57, 711, 1055
MaeII ACGT 1 cut(s) 76
MaeIII GTNAC 2 cut(s) 80, 652
MalI GATC 5 cut(s) 211, 264, 636, 787, 855
MboI GATC 5 cut(s) 209, 262, 634, 785, 853
MfeI CAATTG 1 cut(s) 338
MflI RGATCY 1 cut(s) 209
MluCI AATT 5 cut(s) 326, 338, 610, 798, 934
MlyI GAGTC 3 cut(s) 250, 616, 819
MmeI TCCRAC 2 cut(s) 846, 850
MnlI CCTC 8 cut(s) 82, 85, 88, 94, 97, 296, 1039, 1121
MroI TCCGGA 1 cut(s) 910
MseI TTAA 1 cut(s) 242
MspI CCGG 1 cut(s) 911
MunI CAATTG 1 cut(s) 338
MwoI GCNNNNNNNGC 3 cut(s) 38, 162, 847
NdeII GATC 5 cut(s) 209, 262, 634, 785, 853
NlaIII CATG 4 cut(s) 287, 374, 849, 1076
NlaIV GGNNCC 2 cut(s) 22, 909
NmuCI GTSAC 1 cut(s) 80
NspI RCATGY 1 cut(s) 374
PaeR7I CTCGAG 1 cut(s) 807
PagI TCATGA 1 cut(s) 1072
PciI ACATGT 1 cut(s) 370
PfeI GAWTC 1 cut(s) 914
PkrI GCNGC 4 cut(s) 140, 167, 569, 730
PleI GAGTC 3 cut(s) 250, 616, 818
PpsI GAGTC 3 cut(s) 250, 616, 818
PscI ACATGT 1 cut(s) 370
PspFI CCCAGC 1 cut(s) 134
PspN4I GGNNCC 2 cut(s) 22, 909
PspPI GGNCC 1 cut(s) 1108
PspXI VCTCGAGB 1 cut(s) 807
PstNI CAGNNNCTG 1 cut(s) 677
PsuI RGATCY 1 cut(s) 209
RsaI GTAC 6 cut(s) 22, 216, 715, 805, 984, 1102
RsaNI GTAC 6 cut(s) 21, 215, 714, 804, 983, 1101
SaqAI TTAA 1 cut(s) 242
SatI GCNGC 4 cut(s) 139, 166, 568, 729
Sau3AI GATC 5 cut(s) 209, 262, 634, 785, 853
Sau96I GGNCC 1 cut(s) 1108
ScaI AGTACT 2 cut(s) 805, 1102
SchI GAGTC 3 cut(s) 250, 616, 819
SfaNI GCATC 2 cut(s) 541, 802
Sfr274I CTCGAG 1 cut(s) 807
SinI GGWCC 1 cut(s) 1108
SlaI CTCGAG 1 cut(s) 807
SmlI CTYRAG 1 cut(s) 807
SmoI CTYRAG 1 cut(s) 807
Sse9I AATT 5 cut(s) 326, 338, 610, 798, 934
SsiI CCGC 3 cut(s) 287, 568, 1039
SspI AATATT 1 cut(s) 687
SspMI CTAG 3 cut(s) 57, 711, 1055
StyI CCWWGG 1 cut(s) 557
TaaI ACNGT 4 cut(s) 222, 608, 860, 875
TaiI ACGT 1 cut(s) 79
TaqI TCGA 5 cut(s) 191, 250, 808, 886, 1031
TasI AATT 5 cut(s) 326, 338, 610, 798, 934
TatI WGTACW 3 cut(s) 713, 803, 1100
TauI GCSGC 1 cut(s) 570
TfiI GAWTC 1 cut(s) 914
Tru1I TTAA 1 cut(s) 242
Tru9I TTAA 1 cut(s) 242
TscAI CASTG 3 cut(s) 712, 900, 960
TseFI GTSAC 1 cut(s) 80
TseI GCWGC 3 cut(s) 138, 165, 728
Tsp45I GTSAC 1 cut(s) 80
TspDTI ATGAA 4 cut(s) 17, 272, 359, 1061
TspGWI ACGGA 1 cut(s) 484
TspRI CASTG 3 cut(s) 712, 900, 960
VpaK11BI GGWCC 1 cut(s) 1108
XagI CCTNNNNNAGG 2 cut(s) 528, 835
XceI RCATGY 1 cut(s) 374
XhoI CTCGAG 1 cut(s) 807
XspI CTAG 3 cut(s) 57, 711, 1055
ZrmI AGTACT 2 cut(s) 805, 1102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.