MD07G1281700.v1.1

membrane-associated kinase regulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0009504
Physical Location & Seq
Reverse (-)
34490462 .. 34491762
1301 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1281700.v1.1.491

Sequence Viewer

Length: 405 bp
ATGCCGATATTCTCTGTGTGTTCGCCTAAGAAGGACAAGCAGGGGAACTTTCCGGCGGGATTTAGAGTTGTCTGTAAGAACTTGGGGAAGAGCAAGTCTGCCACGGTGATGGCGGCGCCGCCAGTGCAACGGAGGGACGATTCCTTGCTGCTGCACAACGATGGGATTCAGAGCGCCATTCTACATTGCAAGAGATCGTTCAGTTCTAGAGATTCTTTGTCCTCACTGTCGCGGTCAACCAGTGATTCTTCCTCCATGAAATCATATTCTACTGATTCTTTTCTGTTATCACGGATTGCAAGTGATTCCCATGAAAAATCCGCGAGAATGTCGATCGAAGAAGAAATTTCAAGCTCTGCTCGTCGGATTTTAATGGATAAAACGCGGGAAAAACAGAGGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

135

Amino Acids

14.86

Weight (kDa)

9.89

Isoelectric Point (pI)

83.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 115
AccII CGCG 3 cut(s) 232, 323, 385
AciI CCGC 6 cut(s) 56, 113, 119, 232, 321, 385
AcsI RAATTY 1 cut(s) 345
AcyI GRCGYC 1 cut(s) 116
AgsI TTSAA 1 cut(s) 351
AluBI AGCT 1 cut(s) 354
AluI AGCT 1 cut(s) 354
ApeKI GCWGC 2 cut(s) 148, 151
ApoI RAATTY 1 cut(s) 345
AspLEI GCGC 2 cut(s) 118, 176
AsuHPI GGTGA 1 cut(s) 118
BanI GGYRCC 1 cut(s) 115
BbvI GCAGC 2 cut(s) 135, 138
BccI CCATC 2 cut(s) 103, 155
BfaI CTAG 1 cut(s) 207
BfoI RGCGCY 2 cut(s) 119, 177
BisI GCNGC 4 cut(s) 114, 119, 149, 152
BlsI GCNGC 4 cut(s) 115, 120, 150, 153
BmiI GGNNCC 1 cut(s) 117
BsaHI GRCGYC 1 cut(s) 116
BsaJI CCNNGG 1 cut(s) 102
Bse1I ACTGG 2 cut(s) 122, 240
Bse3DI GCAATG 1 cut(s) 184
BseDI CCNNGG 1 cut(s) 102
BseMI GCAATG 1 cut(s) 184
BseNI ACTGG 2 cut(s) 122, 240
BseXI GCAGC 2 cut(s) 135, 138
BsgI GTGCAG 1 cut(s) 137
Bsh1236I CGCG 3 cut(s) 232, 323, 385
Bsh1285I CGRYCG 1 cut(s) 336
BshNI GGYRCC 1 cut(s) 115
BsiEI CGRYCG 1 cut(s) 336
BsiSI CCGG 1 cut(s) 53
BslFI GGGAC 1 cut(s) 149
BsmFI GGGAC 1 cut(s) 149
Bsp143I GATC 2 cut(s) 194, 333
BspACI CCGC 6 cut(s) 56, 113, 119, 232, 321, 385
BspFNI CGCG 3 cut(s) 232, 323, 385
BspLI GGNNCC 1 cut(s) 117
BspQI GCTCTTC 1 cut(s) 83
BspT107I GGYRCC 1 cut(s) 115
BsrDI GCAATG 1 cut(s) 184
BsrI ACTGG 2 cut(s) 122, 240
BssECI CCNNGG 1 cut(s) 102
BssMI GATC 2 cut(s) 194, 333
BssNI GRCGYC 1 cut(s) 116
Bst4CI ACNGT 2 cut(s) 106, 228
Bst6I CTCTTC 1 cut(s) 83
BstACI GRCGYC 1 cut(s) 116
BstDEI CTNAG 1 cut(s) 27
BstDSI CCRYGG 1 cut(s) 102
BstFNI CGCG 3 cut(s) 232, 323, 385
BstH2I RGCGCY 2 cut(s) 119, 177
BstHHI GCGC 2 cut(s) 118, 176
BstKTI GATC 2 cut(s) 197, 336
BstMBI GATC 2 cut(s) 194, 333
BstMCI CGRYCG 1 cut(s) 336
BstMWI GCNNNNNNNGC 1 cut(s) 124
BstUI CGCG 3 cut(s) 232, 323, 385
BstV1I GCAGC 2 cut(s) 135, 138
BstXI CCANNNNNNTGG 1 cut(s) 109
BtgI CCRYGG 1 cut(s) 102
BtsIMutI CAGTG 3 cut(s) 129, 224, 247
CfoI GCGC 2 cut(s) 118, 176
CviAII CATG 2 cut(s) 256, 311
CviJI RGCY 1 cut(s) 354
CviKI_1 RGCY 1 cut(s) 354
DdeI CTNAG 1 cut(s) 27
DinI GGCGCC 1 cut(s) 117
DpnI GATC 2 cut(s) 196, 335
DpnII GATC 2 cut(s) 194, 333
Eam1104I CTCTTC 1 cut(s) 83
EarI CTCTTC 1 cut(s) 83
EgeI GGCGCC 1 cut(s) 117
EheI GGCGCC 1 cut(s) 117
FaeI CATG 2 cut(s) 259, 314
FaiI YATR 3 cut(s) 257, 265, 312
FaqI GGGAC 1 cut(s) 149
FatI CATG 2 cut(s) 255, 310
FauI CCCGC 2 cut(s) 49, 378
Fnu4HI GCNGC 4 cut(s) 114, 119, 149, 152
Fsp4HI GCNGC 4 cut(s) 114, 119, 149, 152
FspBI CTAG 1 cut(s) 207
GlaI GCGC 2 cut(s) 117, 175
GluI GCNGC 4 cut(s) 114, 119, 149, 152
HaeII RGCGCY 2 cut(s) 119, 177
HapII CCGG 1 cut(s) 53
HhaI GCGC 2 cut(s) 118, 176
Hin1I GRCGYC 1 cut(s) 116
Hin1II CATG 2 cut(s) 259, 314
Hin6I GCGC 2 cut(s) 116, 174
HinP1I GCGC 2 cut(s) 116, 174
HincII GTYRAC 1 cut(s) 237
HindII GTYRAC 1 cut(s) 237
HinfI GANTC 6 cut(s) 140, 166, 212, 245, 275, 305
HpaII CCGG 1 cut(s) 53
HphI GGTGA 1 cut(s) 118
Hpy166II GTNNAC 1 cut(s) 237
Hpy188I TCNGA 2 cut(s) 171, 366
Hpy188III TCNNGA 1 cut(s) 207
Hpy8I GTNNAC 1 cut(s) 237
Hpy99I CGWCG 1 cut(s) 366
HpyAV CCTTC 1 cut(s) 25
HpyCH4III ACNGT 2 cut(s) 106, 228
HpyCH4V TGCA 4 cut(s) 127, 154, 189, 299
HpyF10VI GCNNNNNNNGC 1 cut(s) 124
HpyF3I CTNAG 1 cut(s) 27
Hsp92I GRCGYC 1 cut(s) 116
Hsp92II CATG 2 cut(s) 259, 314
HspAI GCGC 2 cut(s) 116, 174
KasI GGCGCC 1 cut(s) 115
Kzo9I GATC 2 cut(s) 194, 333
LguI GCTCTTC 1 cut(s) 83
LpnPI CCDG 4 cut(s) 26, 66, 135, 253
Lsp1109I GCAGC 2 cut(s) 135, 138
MaeI CTAG 1 cut(s) 207
MalI GATC 2 cut(s) 196, 335
MboI GATC 2 cut(s) 194, 333
MboII GAAGA 4 cut(s) 100, 240, 350, 353
MluCI AATT 1 cut(s) 345
Mly113I GGCGCC 1 cut(s) 116
MmeI TCCRAC 1 cut(s) 344
MnlI CCTC 4 cut(s) 126, 232, 262, 390
MseI TTAA 1 cut(s) 371
MslI CAYNNNNRTG 2 cut(s) 107, 159
MspI CCGG 1 cut(s) 53
MvnI CGCG 3 cut(s) 232, 323, 385
MwoI GCNNNNNNNGC 1 cut(s) 124
NarI GGCGCC 1 cut(s) 116
NdeII GATC 2 cut(s) 194, 333
NlaIII CATG 2 cut(s) 259, 314
NlaIV GGNNCC 1 cut(s) 117
PciSI GCTCTTC 1 cut(s) 83
PfeI GAWTC 6 cut(s) 140, 166, 212, 245, 275, 305
PkrI GCNGC 4 cut(s) 115, 120, 150, 153
Ple19I CGATCG 1 cut(s) 336
PluTI GGCGCC 1 cut(s) 119
PspN4I GGNNCC 1 cut(s) 117
PvuI CGATCG 1 cut(s) 336
RseI CAYNNNNRTG 2 cut(s) 107, 159
SapI GCTCTTC 1 cut(s) 83
SaqAI TTAA 1 cut(s) 371
SatI GCNGC 4 cut(s) 114, 119, 149, 152
Sau3AI GATC 2 cut(s) 194, 333
SetI ASST 1 cut(s) 356
SfoI GGCGCC 1 cut(s) 117
SmiMI CAYNNNNRTG 2 cut(s) 107, 159
Sse9I AATT 1 cut(s) 345
SsiI CCGC 6 cut(s) 56, 113, 119, 232, 321, 385
SspDI GGCGCC 1 cut(s) 115
SspMI CTAG 1 cut(s) 207
TaaI ACNGT 2 cut(s) 106, 228
TaqI TCGA 2 cut(s) 332, 336
TasI AATT 1 cut(s) 345
TauI GCSGC 2 cut(s) 116, 121
TfiI GAWTC 6 cut(s) 140, 166, 212, 245, 275, 305
Tru1I TTAA 1 cut(s) 371
Tru9I TTAA 1 cut(s) 371
TscAI CASTG 3 cut(s) 129, 231, 247
TseI GCWGC 2 cut(s) 148, 151
TspDTI ATGAA 2 cut(s) 272, 327
TspGWI ACGGA 2 cut(s) 145, 307
TspRI CASTG 3 cut(s) 129, 231, 247
XapI RAATTY 1 cut(s) 345
XbaI TCTAGA 1 cut(s) 206
XspI CTAG 1 cut(s) 207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.