Rh1DG423200

membrane-associated kinase regulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0009504
Physical Location & Seq
Reverse (-)
63734876 .. 63737219
2344 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG423200.1

Sequence Viewer

Length: 975 bp
ATGGAGACTCTGTACTTTCTTAAGTTCTGGAAACCCAATCCTTCTCCCATTGCAATTCCCAGTCCTTTGGTAGAAACAGACAACGAATTTGAGGTGGAAGAGGAGGAAGAGGAAGACTCGTTCTTCGATTTGGAGATTAGTACAGAATCAAACACCAAAACCCATGAGGAAAGTTCGAAGCTTTACTCAAAAGAAGCCAAAACAGAACCAACCCTTTATGTTTCTCCTCCTCCTTCTGATCCAATTTCCAAAAGGAAAATCCTCCCAATTGAACCCATTTCCAAGCCTCAGTCCCCCATTGCTCTACTCAAATCAGCTCCAAAGTTTGGGGTGTCGATTTTCAAAAAACCCAAATCAGCTTCAACTAAGAAAACAGGGGAAACAGAGTCCAGCATTTTGATGGAGACCCAGAAGCAGAAAAAGCCAGAAGCCAAAGGCAATGAAGCTTCAAGCCCTTCTAAGCTGACCAGGGACAACAGTTCGAGAAAAACTGGAAGGATATTGCAGAGCCAGAGCTCCGATGATTCGAAAACAGAGCGGTTTTCAAAGGATGTGATAAACAAGTACCTGAAGCTAATCAAGCCTCTGTACTTGAAAGTTTCCAAGAGGTACAGTGACAAGGTGAAGGTTTCCGGCGACTTGCCGATGGCATCGCCGACGTCGTCGCCGGCGGTGTCTATGTACTCCCCTAGGAAGGAGAGGCAGGGGAATATTCCTGCCGGGATTCGAGTTGTTTGTAAGCATCTTGGGAAGAGCAAGTCGGCCACGGCGGCGATGGCGGTGGCGCCGCCGGTGAACCGGAGAGACGACTCGCTGGTGCTTCAAGCAGATGGGATTCAGAGCGCCATTCTGCATTGCAAGAGATCATTCAATTCTAGAGGTACTGAAAATTTCATTTCGTTTTTGCAGTTTGAATTTTCAGGCTCTTCTATAATTTTTAGAGGTACTCCTCAATTTTTAGAGCTGAAGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

324

Amino Acids

36.07

Weight (kDa)

9.38

Isoelectric Point (pI)

63.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 662
AccB1I GGYRCC 1 cut(s) 784
AccB7I CCANNNNNTGG 1 cut(s) 326
AccBSI CCGCTC 1 cut(s) 538
AciI CCGC 5 cut(s) 538, 671, 770, 779, 788
AclWI GGATC 1 cut(s) 233
AcoI YGGCCR 1 cut(s) 762
AcsI RAATTY 3 cut(s) 86, 889, 914
AcuI CTGAAG 1 cut(s) 590
AcyI GRCGYC 2 cut(s) 659, 785
AfaI GTAC 8 cut(s) 14, 142, 566, 590, 611, 683, 883, 946
AfiI CCNNNNNNNGG 2 cut(s) 326, 694
AflII CTTAAG 1 cut(s) 20
AgsI TTSAA 9 cut(s) 272, 343, 363, 450, 546, 595, 824, 871, 914
AjnI CCWGG 1 cut(s) 467
AloI GAACNNNNNNTCC 2 cut(s) 104, 136
AluBI AGCT 8 cut(s) 181, 317, 359, 446, 463, 516, 574, 964
AluI AGCT 8 cut(s) 181, 317, 359, 446, 463, 516, 574, 964
Alw21I GWGCWC 1 cut(s) 518
Alw26I GTCTC 2 cut(s) 398, 798
AlwI GGATC 1 cut(s) 233
AoxI GGCC 1 cut(s) 762
ApoI RAATTY 3 cut(s) 86, 889, 914
ArsI GACNNNNNNTTYG 4 cut(s) 71, 103, 107, 139
AspA2I CCTAGG 1 cut(s) 689
AspLEI GCGC 2 cut(s) 787, 845
AsuC2I CCSGG 1 cut(s) 721
AsuHPI GGTGA 2 cut(s) 634, 805
AsuII TTCGAA 2 cut(s) 176, 527
AvrII CCTAGG 1 cut(s) 689
BanI GGYRCC 1 cut(s) 784
BanII GRGCYC 1 cut(s) 518
BbsI GAAGAC 1 cut(s) 120
Bbv12I GWGCWC 1 cut(s) 518
BccI CCATC 4 cut(s) 394, 640, 769, 824
BceAI ACGGC 1 cut(s) 783
BciT130I CCWGG 1 cut(s) 469
BcnI CCSGG 1 cut(s) 721
BcoDI GTCTC 2 cut(s) 398, 798
BfaI CTAG 2 cut(s) 690, 876
BfoI RGCGCY 2 cut(s) 788, 846
BfrI CTTAAG 1 cut(s) 20
BglI GCCNNNNNGGC 1 cut(s) 770
BisI GCNGC 2 cut(s) 771, 788
BlnI CCTAGG 1 cut(s) 689
BlsI GCNGC 2 cut(s) 772, 789
Bme1390I CCNGG 2 cut(s) 469, 721
BmiI GGNNCC 1 cut(s) 786
BmrFI CCNGG 2 cut(s) 469, 721
BmrI ACTGGG 1 cut(s) 54
BmsI GCATC 2 cut(s) 659, 751
BmuI ACTGGG 1 cut(s) 54
BpiI GAAGAC 1 cut(s) 120
BplI GAGNNNNNCTC 2 cut(s) 101, 133
Bpu14I TTCGAA 2 cut(s) 176, 527
BpuMI CCSGG 1 cut(s) 721
BsaHI GRCGYC 2 cut(s) 659, 785
BsaI GGTCTC 1 cut(s) 398
BsaJI CCNNGG 3 cut(s) 468, 689, 765
BsaWI WCCGGW 1 cut(s) 798
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 2 cut(s) 326, 694
Bse118I RCCGGY 2 cut(s) 667, 790
Bse1I ACTGG 2 cut(s) 60, 496
Bse3DI GCAATG 4 cut(s) 48, 297, 445, 853
BseBI CCWGG 1 cut(s) 469
BseDI CCNNGG 3 cut(s) 468, 689, 765
BseGI GGATG 1 cut(s) 556
BseLI CCNNNNNNNGG 2 cut(s) 326, 694
BseMI GCAATG 4 cut(s) 48, 297, 445, 853
BseMII CTCAG 1 cut(s) 302
BseNI ACTGG 2 cut(s) 60, 496
BseRI GAGGAG 4 cut(s) 116, 216, 219, 939
BshFI GGCC 1 cut(s) 764
BshNI GGYRCC 1 cut(s) 784
BsiHKAI GWGCWC 1 cut(s) 518
BsiSI CCGG 5 cut(s) 633, 668, 720, 791, 799
BslFI GGGAC 2 cut(s) 277, 485
BslI CCNNNNNNNGG 2 cut(s) 326, 694
BsmAI GTCTC 2 cut(s) 398, 798
BsmBI CGTCTC 1 cut(s) 798
BsmFI GGGAC 2 cut(s) 277, 485
BsnI GGCC 1 cut(s) 764
Bso31I GGTCTC 1 cut(s) 398
Bsp119I TTCGAA 2 cut(s) 176, 527
Bsp1286I GDGCHC 1 cut(s) 518
Bsp143I GATC 2 cut(s) 238, 863
BspACI CCGC 5 cut(s) 538, 671, 770, 779, 788
BspANI GGCC 1 cut(s) 764
BspCNI CTCAG 1 cut(s) 301
BspLI GGNNCC 1 cut(s) 786
BspPI GGATC 1 cut(s) 233
BspQI GCTCTTC 2 cut(s) 746, 931
BspT104I TTCGAA 2 cut(s) 176, 527
BspT107I GGYRCC 1 cut(s) 784
BspTI CTTAAG 1 cut(s) 20
BspTNI GGTCTC 1 cut(s) 398
BsrBI CCGCTC 1 cut(s) 538
BsrDI GCAATG 4 cut(s) 48, 297, 445, 853
BsrFI RCCGGY 2 cut(s) 667, 790
BsrI ACTGG 2 cut(s) 60, 496
BssAI RCCGGY 2 cut(s) 667, 790
BssECI CCNNGG 3 cut(s) 468, 689, 765
BssMI GATC 2 cut(s) 238, 863
BssNI GRCGYC 2 cut(s) 659, 785
BssT1I CCWWGG 1 cut(s) 689
Bst2UI CCWGG 1 cut(s) 469
Bst4CI ACNGT 2 cut(s) 479, 614
Bst6I CTCTTC 4 cut(s) 93, 102, 746, 931
BstACI GRCGYC 2 cut(s) 659, 785
BstAFI CTTAAG 1 cut(s) 20
BstBI TTCGAA 2 cut(s) 176, 527
BstC8I GCNNGC 1 cut(s) 669
BstDEI CTNAG 3 cut(s) 288, 366, 459
BstDSI CCRYGG 1 cut(s) 765
BstF5I GGATG 1 cut(s) 556
BstH2I RGCGCY 2 cut(s) 788, 846
BstHHI GCGC 2 cut(s) 787, 845
BstKTI GATC 2 cut(s) 241, 866
BstMAI GTCTC 2 cut(s) 398, 798
BstMBI GATC 2 cut(s) 238, 863
BstMWI GCNNNNNNNGC 4 cut(s) 421, 580, 770, 776
BstNI CCWGG 1 cut(s) 469
BstSCI CCNGG 2 cut(s) 467, 719
BstV2I GAAGAC 1 cut(s) 120
BstXI CCANNNNNNTGG 1 cut(s) 67
BsuRI GGCC 1 cut(s) 764
BtgI CCRYGG 1 cut(s) 765
BtgZI GCGATG 2 cut(s) 636, 788
BtsCI GGATG 1 cut(s) 556
BtsIMutI CAGTG 1 cut(s) 619
Cac8I GCNNGC 1 cut(s) 669
CfoI GCGC 2 cut(s) 787, 845
Cfr10I RCCGGY 2 cut(s) 667, 790
Csp6I GTAC 8 cut(s) 13, 141, 565, 589, 610, 682, 882, 945
CviAII CATG 1 cut(s) 164
CviQI GTAC 8 cut(s) 13, 141, 565, 589, 610, 682, 882, 945
DdeI CTNAG 3 cut(s) 288, 366, 459
DinI GGCGCC 1 cut(s) 786
DpnI GATC 2 cut(s) 240, 865
DpnII GATC 2 cut(s) 238, 863
EaeI YGGCCR 1 cut(s) 762
Eam1104I CTCTTC 4 cut(s) 93, 102, 746, 931
EarI CTCTTC 4 cut(s) 93, 102, 746, 931
Ecl136II GAGCTC 1 cut(s) 516
Eco130I CCWWGG 1 cut(s) 689
Eco24I GRGCYC 1 cut(s) 518
Eco31I GGTCTC 1 cut(s) 398
Eco53kI GAGCTC 1 cut(s) 516
Eco57I CTGAAG 1 cut(s) 590
EcoICRI GAGCTC 1 cut(s) 516
EcoRII CCWGG 1 cut(s) 467
EcoT14I CCWWGG 1 cut(s) 689
EcoT38I GRGCYC 1 cut(s) 518
EgeI GGCGCC 1 cut(s) 786
EheI GGCGCC 1 cut(s) 786
ErhI CCWWGG 1 cut(s) 689
Esp3I CGTCTC 1 cut(s) 798
FaeI CATG 1 cut(s) 167
FaiI YATR 4 cut(s) 165, 219, 680, 932
FaqI GGGAC 2 cut(s) 277, 485
FatI CATG 1 cut(s) 163
Fnu4HI GCNGC 2 cut(s) 771, 788
FokI GGATG 1 cut(s) 563
FriOI GRGCYC 1 cut(s) 518
Fsp4HI GCNGC 2 cut(s) 771, 788
FspBI CTAG 2 cut(s) 690, 876
GlaI GCGC 2 cut(s) 786, 844
GluI GCNGC 2 cut(s) 771, 788
HaeII RGCGCY 2 cut(s) 788, 846
HaeIII GGCC 1 cut(s) 764
HapII CCGG 5 cut(s) 633, 668, 720, 791, 799
HhaI GCGC 2 cut(s) 787, 845
Hin1I GRCGYC 2 cut(s) 659, 785
Hin1II CATG 1 cut(s) 167
Hin6I GCGC 2 cut(s) 785, 843
HinP1I GCGC 2 cut(s) 785, 843
HindIII AAGCTT 2 cut(s) 179, 444
HinfI GANTC 8 cut(s) 7, 116, 146, 386, 524, 724, 809, 835
HpaII CCGG 5 cut(s) 633, 668, 720, 791, 799
HphI GGTGA 2 cut(s) 634, 805
Hpy166II GTNNAC 1 cut(s) 796
Hpy188I TCNGA 3 cut(s) 238, 520, 840
Hpy188III TCNNGA 3 cut(s) 28, 483, 876
Hpy8I GTNNAC 1 cut(s) 796
Hpy99I CGWCG 3 cut(s) 661, 664, 667
HpyAV CCTTC 6 cut(s) 51, 243, 465, 489, 619, 688
HpyCH4III ACNGT 2 cut(s) 479, 614
HpyCH4IV ACGT 1 cut(s) 659
HpyCH4V TGCA 5 cut(s) 53, 505, 853, 858, 907
HpyF10VI GCNNNNNNNGC 4 cut(s) 421, 580, 770, 776
HpyF3I CTNAG 3 cut(s) 288, 366, 459
HpySE526I ACGT 1 cut(s) 659
Hsp92I GRCGYC 2 cut(s) 659, 785
Hsp92II CATG 1 cut(s) 167
HspAI GCGC 2 cut(s) 785, 843
KasI GGCGCC 1 cut(s) 784
KroI GCCGGC 1 cut(s) 667
KroNI GCCGGC 1 cut(s) 669
Kzo9I GATC 2 cut(s) 238, 863
LguI GCTCTTC 2 cut(s) 746, 931
LmnI GCTCC 2 cut(s) 322, 521
LweI GCATC 2 cut(s) 659, 751
MaeI CTAG 2 cut(s) 690, 876
MaeII ACGT 1 cut(s) 659
MaeIII GTNAC 1 cut(s) 614
MalI GATC 2 cut(s) 240, 865
MbiI CCGCTC 1 cut(s) 538
MboI GATC 2 cut(s) 238, 863
MboII GAAGA 6 cut(s) 110, 115, 119, 125, 763, 918
MfeI CAATTG 1 cut(s) 267
MhlI GDGCHC 1 cut(s) 518
MluCI AATT 9 cut(s) 54, 86, 243, 267, 871, 889, 914, 933, 953
Mly113I GGCGCC 1 cut(s) 785
MlyI GAGTC 3 cut(s) 110, 395, 803
MreI CGCCGGCG 1 cut(s) 667
MroNI GCCGGC 1 cut(s) 667
MseI TTAA 1 cut(s) 21
MslI CAYNNNNRTG 1 cut(s) 398
MspCI CTTAAG 1 cut(s) 20
MspI CCGG 5 cut(s) 633, 668, 720, 791, 799
MspR9I CCNGG 2 cut(s) 469, 721
MunI CAATTG 1 cut(s) 267
MvaI CCWGG 1 cut(s) 469
MwoI GCNNNNNNNGC 4 cut(s) 421, 580, 770, 776
NaeI GCCGGC 1 cut(s) 669
NarI GGCGCC 1 cut(s) 785
NciI CCSGG 1 cut(s) 721
NdeII GATC 2 cut(s) 238, 863
NgoMIV GCCGGC 1 cut(s) 667
NlaIII CATG 1 cut(s) 167
NlaIV GGNNCC 1 cut(s) 786
NmuCI GTSAC 1 cut(s) 614
NspV TTCGAA 2 cut(s) 176, 527
PciSI GCTCTTC 2 cut(s) 746, 931
PcsI WCGNNNNNNNCGW 1 cut(s) 659
PdiI GCCGGC 1 cut(s) 669
PfeI GAWTC 4 cut(s) 146, 524, 724, 835
PflFI GACNNNGTC 1 cut(s) 661
PflMI CCANNNNNTGG 1 cut(s) 326
PkrI GCNGC 2 cut(s) 772, 789
PleI GAGTC 3 cut(s) 110, 394, 803
PluTI GGCGCC 1 cut(s) 788
PpsI GAGTC 3 cut(s) 110, 394, 803
Psp124BI GAGCTC 1 cut(s) 518
Psp6I CCWGG 1 cut(s) 467
PspGI CCWGG 1 cut(s) 467
PspN4I GGNNCC 1 cut(s) 786
PsyI GACNNNGTC 1 cut(s) 661
RsaI GTAC 8 cut(s) 14, 142, 566, 590, 611, 683, 883, 946
RsaNI GTAC 8 cut(s) 13, 141, 565, 589, 610, 682, 882, 945
RseI CAYNNNNRTG 1 cut(s) 398
SacI GAGCTC 1 cut(s) 518
SapI GCTCTTC 2 cut(s) 746, 931
SaqAI TTAA 1 cut(s) 21
SatI GCNGC 2 cut(s) 771, 788
Sau3AI GATC 2 cut(s) 238, 863
SchI GAGTC 3 cut(s) 110, 395, 803
ScrFI CCNGG 2 cut(s) 469, 721
SduI GDGCHC 1 cut(s) 518
SfaNI GCATC 2 cut(s) 659, 751
SfoI GGCGCC 1 cut(s) 786
SfuI TTCGAA 2 cut(s) 176, 527
SgrAI CRCCGGYG 2 cut(s) 667, 790
SmiMI CAYNNNNRTG 1 cut(s) 398
SmlI CTYRAG 1 cut(s) 20
SmoI CTYRAG 1 cut(s) 20
Sse9I AATT 9 cut(s) 54, 86, 243, 267, 871, 889, 914, 933, 953
SsiI CCGC 5 cut(s) 538, 671, 770, 779, 788
SspDI GGCGCC 1 cut(s) 784
SspI AATATT 1 cut(s) 712
SspMI CTAG 2 cut(s) 690, 876
SstI GAGCTC 1 cut(s) 518
StyD4I CCNGG 2 cut(s) 467, 719
StyI CCWWGG 1 cut(s) 689
TaaI ACNGT 2 cut(s) 479, 614
TaiI ACGT 1 cut(s) 662
TaqI TCGA 6 cut(s) 126, 176, 335, 482, 527, 727
TasI AATT 9 cut(s) 54, 86, 243, 267, 871, 889, 914, 933, 953
TatI WGTACW 4 cut(s) 12, 140, 588, 681
TauI GCSGC 2 cut(s) 773, 790
TfiI GAWTC 4 cut(s) 146, 524, 724, 835
Tru1I TTAA 1 cut(s) 21
Tru9I TTAA 1 cut(s) 21
TscAI CASTG 1 cut(s) 619
TseFI GTSAC 1 cut(s) 614
Tsp45I GTSAC 1 cut(s) 614
TspDTI ATGAA 2 cut(s) 456, 883
TspRI CASTG 1 cut(s) 619
Tth111I GACNNNGTC 1 cut(s) 661
Van91I CCANNNNNTGG 1 cut(s) 326
Vha464I CTTAAG 1 cut(s) 20
XapI RAATTY 3 cut(s) 86, 889, 914
XbaI TCTAGA 1 cut(s) 875
XcmI CCANNNNNNNNNTGG 2 cut(s) 397, 772
XmaJI CCTAGG 1 cut(s) 689
XspI CTAG 2 cut(s) 690, 876
ZraI GACGTC 1 cut(s) 660
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.