pycom01g22150

membrane-associated kinase regulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0009504
Physical Location & Seq
Reverse (-)
19868216 .. 19869438
1223 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g22150.2

Sequence Viewer

Length: 1047 bp
ATGGAAACTCTGTACTTCCTCAAGTTCTGGAAACCCAATGCCTCCACCGCCACACCTCCAAACTCCTCCGTCGCTAAAGAAAACGACGTCGAAGAAGAAGAAGAAGATTCTTTCTTCGATTTGGAATTAAGTTTCGACCTCATCCAAACCCCACAGGCAAACAACATCTTCGAATCCAAACCAAAATCAAACACCTCGGACAAAACAGCCGGCAAACCAACCCTTTCTCTGTCCACTTCCGACCCGATTTCCAAAAGAAAAATTCTCCCGATCGAGCCAGTTTCCAAGCCTCCTCAGTCCCCCATCGCTCTCCTTAAATCCGCCCCAAAGTTCGGAGCTTTAATGCTCAAAAAACCCAGACAGAAAATAGAGAAAAAAACGGGGGAAAAAGCAGAGCCCGAGGTTTCGATGGAGTCCCACAAGCAGAAAACTAAAGTAGTAATTGCGAAACAGCTCGCCGGAGAAGAGGCTCAAAACCCAACCAAGCTCACCCGTGAAAACAGCTCCAGAAGAAGCGGAATCGGATTGCAGAATCCGAGGATGGAAAACCAGAAAACAGAGAGATTTTGCAAGGATGTTTTGCAGAAGTACCTTAATCTGATCAAACCTCTGTACTCGAAGGTTTCCAGGAGCTCTACTGAGAAGGTGAAACCTTCTGTAGACTCACCGACGGCGTCGCCCGCGATGCCGATATTCTCTATGTGTTCGCCCAAAAAGGACAAACAGGGGAACTTTCCGGCGGGATTTAGAGTTGTCTCTAAGAACTTGGGGAAAAGCAAGTCGGCCACGGCGATGGTGGCGCCGCCGGTGCAACGGAGGGACGATTCCTTGCTGCTGCACAACGATGGGATTCAGAGCGCCATTCTGCATTGCAAGAGATCGTTCAACTCTAGAGATTCCTTGTCTTCGTTGTCGCGATCAACCAGTGATTCCTCCTCTACGAAATCATATTCTACAGATTGTTCTCTGTTGTCACGGTTTGCAAGTGAATCCCACGACAAATCAGCAAGAAACTCGATCGAGGATGGGTACGGTGCCGAAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

349

Amino Acids

38.42

Weight (kDa)

9.47

Isoelectric Point (pI)

60.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 90
AccB1I GGYRCC 2 cut(s) 799, 1034
AccI GTMKAC 1 cut(s) 660
AccII CGCG 2 cut(s) 683, 916
AciI CCGC 6 cut(s) 48, 321, 516, 681, 740, 803
AcoI YGGCCR 1 cut(s) 783
AcsI RAATTY 1 cut(s) 261
AcyI GRCGYC 3 cut(s) 87, 674, 800
AfaI GTAC 4 cut(s) 14, 590, 614, 1031
AfiI CCNNNNNNNGG 1 cut(s) 332
AgsI TTSAA 1 cut(s) 886
AjnI CCWGG 1 cut(s) 626
AluBI AGCT 5 cut(s) 338, 454, 487, 504, 633
AluI AGCT 5 cut(s) 338, 454, 487, 504, 633
Alw21I GWGCWC 1 cut(s) 635
Alw26I GTCTC 1 cut(s) 760
Ama87I CYCGRG 1 cut(s) 398
AoxI GGCC 1 cut(s) 783
ApeKI GCWGC 2 cut(s) 832, 835
ApoI RAATTY 1 cut(s) 261
AspLEI GCGC 2 cut(s) 802, 860
AsuHPI GGTGA 3 cut(s) 481, 657, 658
AsuII TTCGAA 1 cut(s) 171
AvaI CYCGRG 1 cut(s) 398
BanI GGYRCC 2 cut(s) 799, 1034
BanII GRGCYC 2 cut(s) 399, 635
BbsI GAAGAC 1 cut(s) 897
Bbv12I GWGCWC 1 cut(s) 635
BbvI GCAGC 2 cut(s) 819, 822
BccI CCATC 6 cut(s) 311, 403, 535, 787, 839, 1019
BceAI ACGGC 2 cut(s) 687, 804
BcgI CGANNNNNNTGC 2 cut(s) 996, 1030
BciT130I CCWGG 1 cut(s) 628
BclI TGATCA 1 cut(s) 600
BcoDI GTCTC 1 cut(s) 760
BfaI CTAG 1 cut(s) 891
BfmI CTRYAG 2 cut(s) 657, 954
BfoI RGCGCY 2 cut(s) 803, 861
BisI GCNGC 3 cut(s) 803, 833, 836
BlsI GCNGC 3 cut(s) 804, 834, 837
Bme1390I CCNGG 1 cut(s) 628
BmeT110I CYCGRG 1 cut(s) 398
BmiI GGNNCC 2 cut(s) 801, 1036
BmrFI CCNGG 1 cut(s) 628
BmsI GCATC 1 cut(s) 675
BpiI GAAGAC 1 cut(s) 897
BpmI CTGGAG 1 cut(s) 490
Bpu14I TTCGAA 1 cut(s) 171
BpuEI CTTGAG 1 cut(s) 5
BsaHI GRCGYC 3 cut(s) 87, 674, 800
BsaJI CCNNGG 4 cut(s) 195, 399, 536, 786
Bsc4I CCNNNNNNNGG 1 cut(s) 332
Bse118I RCCGGY 2 cut(s) 209, 805
Bse1I ACTGG 2 cut(s) 278, 924
Bse3DI GCAATG 1 cut(s) 868
BseBI CCWGG 1 cut(s) 628
BseDI CCNNGG 4 cut(s) 195, 399, 536, 786
BseGI GGATG 4 cut(s) 141, 546, 580, 1030
BseLI CCNNNNNNNGG 1 cut(s) 332
BseMI GCAATG 1 cut(s) 868
BseMII CTCAG 2 cut(s) 308, 630
BseNI ACTGG 2 cut(s) 278, 924
BseRI GAGGAG 3 cut(s) 55, 282, 925
BseXI GCAGC 2 cut(s) 819, 822
BsgI GTGCAG 1 cut(s) 821
Bsh1236I CGCG 2 cut(s) 683, 916
Bsh1285I CGRYCG 2 cut(s) 273, 1020
BshFI GGCC 1 cut(s) 785
BshNI GGYRCC 2 cut(s) 799, 1034
BsiEI CGRYCG 2 cut(s) 273, 1020
BsiHKAI GWGCWC 1 cut(s) 635
BsiHKCI CYCGRG 1 cut(s) 398
BsiSI CCGG 4 cut(s) 210, 459, 737, 806
BslFI GGGAC 3 cut(s) 283, 400, 833
BslI CCNNNNNNNGG 1 cut(s) 332
BsmAI GTCTC 1 cut(s) 760
BsmFI GGGAC 3 cut(s) 283, 400, 833
BsnI GGCC 1 cut(s) 785
BsoBI CYCGRG 1 cut(s) 398
Bsp119I TTCGAA 1 cut(s) 171
Bsp1286I GDGCHC 2 cut(s) 399, 635
Bsp143I GATC 5 cut(s) 270, 600, 878, 917, 1017
Bsp68I TCGCGA 1 cut(s) 916
BspACI CCGC 6 cut(s) 48, 321, 516, 681, 740, 803
BspANI GGCC 1 cut(s) 785
BspCNI CTCAG 2 cut(s) 307, 631
BspFNI CGCG 2 cut(s) 683, 916
BspLI GGNNCC 2 cut(s) 801, 1036
BspT104I TTCGAA 1 cut(s) 171
BspT107I GGYRCC 2 cut(s) 799, 1034
BsrDI GCAATG 1 cut(s) 868
BsrFI RCCGGY 2 cut(s) 209, 805
BsrI ACTGG 2 cut(s) 278, 924
BssAI RCCGGY 2 cut(s) 209, 805
BssECI CCNNGG 4 cut(s) 195, 399, 536, 786
BssMI GATC 5 cut(s) 270, 600, 878, 917, 1017
BssNI GRCGYC 3 cut(s) 87, 674, 800
Bst2UI CCWGG 1 cut(s) 628
Bst4CI ACNGT 2 cut(s) 978, 1034
Bst6I CTCTTC 1 cut(s) 459
BstACI GRCGYC 3 cut(s) 87, 674, 800
BstBI TTCGAA 1 cut(s) 171
BstC8I GCNNGC 3 cut(s) 211, 456, 681
BstDEI CTNAG 3 cut(s) 294, 639, 759
BstDSI CCRYGG 1 cut(s) 786
BstF5I GGATG 4 cut(s) 141, 546, 580, 1030
BstFNI CGCG 2 cut(s) 683, 916
BstH2I RGCGCY 2 cut(s) 803, 861
BstHHI GCGC 2 cut(s) 802, 860
BstKTI GATC 5 cut(s) 273, 603, 881, 920, 1020
BstMAI GTCTC 1 cut(s) 760
BstMBI GATC 5 cut(s) 270, 600, 878, 917, 1017
BstMCI CGRYCG 2 cut(s) 273, 1020
BstMWI GCNNNNNNNGC 5 cut(s) 47, 680, 685, 797, 808
BstNI CCWGG 1 cut(s) 628
BstSCI CCNGG 1 cut(s) 626
BstSFI CTRYAG 2 cut(s) 657, 954
BstUI CGCG 2 cut(s) 683, 916
BstV1I GCAGC 2 cut(s) 819, 822
BstV2I GAAGAC 1 cut(s) 897
BstXI CCANNNNNNTGG 1 cut(s) 793
BsuRI GGCC 1 cut(s) 785
BtgI CCRYGG 1 cut(s) 786
BtgZI GCGATG 3 cut(s) 289, 698, 806
BtsCI GGATG 4 cut(s) 141, 546, 580, 1030
BtsIMutI CAGTG 1 cut(s) 931
BtuMI TCGCGA 1 cut(s) 916
Cac8I GCNNGC 3 cut(s) 211, 456, 681
CfoI GCGC 2 cut(s) 802, 860
Cfr10I RCCGGY 2 cut(s) 209, 805
CseI GACGC 1 cut(s) 663
Csp6I GTAC 4 cut(s) 13, 589, 613, 1030
CviQI GTAC 4 cut(s) 13, 589, 613, 1030
DdeI CTNAG 3 cut(s) 294, 639, 759
DinI GGCGCC 1 cut(s) 801
DpnI GATC 5 cut(s) 272, 602, 880, 919, 1019
DpnII GATC 5 cut(s) 270, 600, 878, 917, 1017
EaeI YGGCCR 1 cut(s) 783
Eam1104I CTCTTC 1 cut(s) 459
EarI CTCTTC 1 cut(s) 459
EciI GGCGGA 1 cut(s) 310
Ecl136II GAGCTC 1 cut(s) 633
Eco24I GRGCYC 2 cut(s) 399, 635
Eco53kI GAGCTC 1 cut(s) 633
Eco88I CYCGRG 1 cut(s) 398
EcoICRI GAGCTC 1 cut(s) 633
EcoRII CCWGG 1 cut(s) 626
EcoT38I GRGCYC 2 cut(s) 399, 635
EgeI GGCGCC 1 cut(s) 801
EheI GGCGCC 1 cut(s) 801
FaiI YATR 2 cut(s) 701, 949
FaqI GGGAC 3 cut(s) 283, 400, 833
FauI CCCGC 2 cut(s) 688, 733
FbaI TGATCA 1 cut(s) 600
FblI GTMKAC 1 cut(s) 660
Fnu4HI GCNGC 3 cut(s) 803, 833, 836
FokI GGATG 4 cut(s) 128, 553, 587, 1037
FriOI GRGCYC 2 cut(s) 399, 635
Fsp4HI GCNGC 3 cut(s) 803, 833, 836
FspBI CTAG 1 cut(s) 891
GlaI GCGC 2 cut(s) 801, 859
GluI GCNGC 3 cut(s) 803, 833, 836
GsuI CTGGAG 1 cut(s) 490
HaeII RGCGCY 2 cut(s) 803, 861
HaeIII GGCC 1 cut(s) 785
HapII CCGG 4 cut(s) 210, 459, 737, 806
HgaI GACGC 1 cut(s) 663
HhaI GCGC 2 cut(s) 802, 860
Hin1I GRCGYC 3 cut(s) 87, 674, 800
Hin6I GCGC 2 cut(s) 800, 858
HinP1I GCGC 2 cut(s) 800, 858
HpaII CCGG 4 cut(s) 210, 459, 737, 806
HphI GGTGA 3 cut(s) 481, 657, 658
Hpy166II GTNNAC 2 cut(s) 234, 661
Hpy188I TCNGA 8 cut(s) 199, 241, 335, 524, 537, 600, 855, 1046
Hpy188III TCNNGA 5 cut(s) 28, 268, 507, 891, 915
Hpy8I GTNNAC 2 cut(s) 234, 661
Hpy99I CGWCG 5 cut(s) 74, 89, 92, 673, 679
HpyAV CCTTC 3 cut(s) 613, 637, 663
HpyCH4III ACNGT 2 cut(s) 978, 1034
HpyCH4IV ACGT 1 cut(s) 87
HpyCH4V TGCA 8 cut(s) 529, 570, 583, 811, 838, 868, 873, 983
HpyF10VI GCNNNNNNNGC 5 cut(s) 47, 680, 685, 797, 808
HpyF3I CTNAG 3 cut(s) 294, 639, 759
HpySE526I ACGT 1 cut(s) 87
Hsp92I GRCGYC 3 cut(s) 87, 674, 800
HspAI GCGC 2 cut(s) 800, 858
KasI GGCGCC 1 cut(s) 799
KroI GCCGGC 1 cut(s) 209
KroNI GCCGGC 1 cut(s) 211
Ksp22I TGATCA 1 cut(s) 600
Kzo9I GATC 5 cut(s) 270, 600, 878, 917, 1017
LmnI GCTCC 3 cut(s) 335, 509, 630
Lsp1109I GCAGC 2 cut(s) 819, 822
LweI GCATC 1 cut(s) 675
MaeI CTAG 1 cut(s) 891
MaeII ACGT 1 cut(s) 87
MaeIII GTNAC 1 cut(s) 972
MalI GATC 5 cut(s) 272, 602, 880, 919, 1019
MboI GATC 5 cut(s) 270, 600, 878, 917, 1017
MhlI GDGCHC 2 cut(s) 399, 635
MluCI AATT 3 cut(s) 125, 261, 441
Mly113I GGCGCC 1 cut(s) 800
MlyI GAGTC 2 cut(s) 422, 656
MmeI TCCRAC 1 cut(s) 264
MroNI GCCGGC 1 cut(s) 209
MseI TTAA 4 cut(s) 128, 315, 341, 594
MslI CAYNNNNRTG 2 cut(s) 791, 843
MspI CCGG 4 cut(s) 210, 459, 737, 806
MspR9I CCNGG 1 cut(s) 628
MvaI CCWGG 1 cut(s) 628
MvnI CGCG 2 cut(s) 683, 916
MwoI GCNNNNNNNGC 5 cut(s) 47, 680, 685, 797, 808
NaeI GCCGGC 1 cut(s) 211
NarI GGCGCC 1 cut(s) 800
NdeII GATC 5 cut(s) 270, 600, 878, 917, 1017
NgoMIV GCCGGC 1 cut(s) 209
NlaIV GGNNCC 2 cut(s) 801, 1036
NmuCI GTSAC 1 cut(s) 972
NruI TCGCGA 1 cut(s) 916
NspV TTCGAA 1 cut(s) 171
PcsI WCGNNNNNNNCGW 1 cut(s) 788
PdiI GCCGGC 1 cut(s) 211
PfeI GAWTC 9 cut(s) 107, 173, 519, 532, 824, 850, 896, 929, 989
PflFI GACNNNGTC 1 cut(s) 673
PfoI TCCNGGA 1 cut(s) 626
PkrI GCNGC 3 cut(s) 804, 834, 837
Ple19I CGATCG 2 cut(s) 273, 1020
PleI GAGTC 2 cut(s) 421, 656
PluTI GGCGCC 1 cut(s) 803
PpsI GAGTC 2 cut(s) 421, 656
Psp124BI GAGCTC 1 cut(s) 635
Psp6I CCWGG 1 cut(s) 626
PspGI CCWGG 1 cut(s) 626
PspN4I GGNNCC 2 cut(s) 801, 1036
PsyI GACNNNGTC 1 cut(s) 673
PvuI CGATCG 2 cut(s) 273, 1020
RruI TCGCGA 1 cut(s) 916
RsaI GTAC 4 cut(s) 14, 590, 614, 1031
RsaNI GTAC 4 cut(s) 13, 589, 613, 1030
RseI CAYNNNNRTG 2 cut(s) 791, 843
SacI GAGCTC 1 cut(s) 635
SaqAI TTAA 4 cut(s) 128, 315, 341, 594
SatI GCNGC 3 cut(s) 803, 833, 836
Sau3AI GATC 5 cut(s) 270, 600, 878, 917, 1017
SchI GAGTC 2 cut(s) 422, 656
ScrFI CCNGG 1 cut(s) 628
SduI GDGCHC 2 cut(s) 399, 635
SfaNI GCATC 1 cut(s) 675
SfcI CTRYAG 2 cut(s) 657, 954
SfoI GGCGCC 1 cut(s) 801
SfuI TTCGAA 1 cut(s) 171
SgrAI CRCCGGYG 1 cut(s) 805
SmiMI CAYNNNNRTG 2 cut(s) 791, 843
SmlI CTYRAG 1 cut(s) 20
SmoI CTYRAG 1 cut(s) 20
Sse9I AATT 3 cut(s) 125, 261, 441
SsiI CCGC 6 cut(s) 48, 321, 516, 681, 740, 803
SspDI GGCGCC 1 cut(s) 799
SspMI CTAG 1 cut(s) 891
SstI GAGCTC 1 cut(s) 635
StyD4I CCNGG 1 cut(s) 626
TaaI ACNGT 2 cut(s) 978, 1034
TaiI ACGT 1 cut(s) 90
TaqI TCGA 9 cut(s) 90, 117, 135, 171, 273, 407, 617, 1016, 1020
TasI AATT 3 cut(s) 125, 261, 441
TatI WGTACW 2 cut(s) 12, 612
TauI GCSGC 1 cut(s) 805
TfiI GAWTC 9 cut(s) 107, 173, 519, 532, 824, 850, 896, 929, 989
Tru1I TTAA 4 cut(s) 128, 315, 341, 594
Tru9I TTAA 4 cut(s) 128, 315, 341, 594
TscAI CASTG 1 cut(s) 931
TseFI GTSAC 1 cut(s) 972
TseI GCWGC 2 cut(s) 832, 835
Tsp45I GTSAC 1 cut(s) 972
TspGWI ACGGA 2 cut(s) 58, 829
TspRI CASTG 1 cut(s) 931
Tth111I GACNNNGTC 1 cut(s) 673
XapI RAATTY 1 cut(s) 261
XbaI TCTAGA 1 cut(s) 890
XcmI CCANNNNNNNNNTGG 1 cut(s) 793
XmiI GTMKAC 1 cut(s) 660
XspI CTAG 1 cut(s) 891
ZraI GACGTC 1 cut(s) 88
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.