pycom07g25570

membrane-associated kinase regulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0009504
Physical Location & Seq
Reverse (-)
25842401 .. 25843642
1242 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g25570.2

Sequence Viewer

Length: 1038 bp
ATGGCAACTCTGTACTTCCTCAAGTTCTGGAAACCCAATGCCTCCACCGGCACACCTCCAAATGCTTCCATTGCAAAAGACAACGAGGTCGAAGAGGAAGAGGAAGATTCTTTCTTCGACTTGGAATTAAGTTTTGATATCATCCAAAGTTCACAAGAAAACAACATCCTCGACTTCAAACCAAAACCAAACACCTCCGACAAAACAGCCGGCGGCAAACCAACCCTTTCTCTGTCCGCTTCCGATCCCATTTCCAAAAGAAAAATCCTCCCTATTGAGCCAGTTTCCAGGCCTCCTCAGTCCCCCATCGCTCTCCTTAAATCCGCCCCAAAGTTCGGAGCTTTAAAGTTCAAAAATCCCAGACAGAAAACAGATCAAAAAACAGGGGAAAAAGGTGAGTCGGAGGTTTCGATGGAGTCCCAGAAGCAGAAAATCAAAGGAGTAATTGCGAAACAGCTCGCCGGAGAAGAAGCTCAAAACCCACCTAAGCTCACCCGAGAAAACAGCTCCAGGAGAAACGGAATGGGATTGCAGAATCTGAAGCCGGAAGACTCGAAAACAGAGAGATTTTCTAAGGATGTTCTGCAGAAGTACCTTAATCTAATCAAACCTCTGTACTCCAAGGTTCCCAGGAGGTCTACTGAGAAGGTGAAGCCCGCCGGAGATTCACCGACGGCGTCTCCGGCGATGCCGATATTCTCTATGTGTTCGCCGAAGAAGGACAAGCAGGGGAACTTTCCGGCGGGATTTAGAGTAGTCTGTAAGAAATTGGGGAAGAGCAAGTCTGCCACGGTGATGGCGGCGCCGCCGGTGCAACGGAGGGACGATTCCTTGCTGCTGCACAACGATGGGATTCAGAGCGCCATTCTACATTGCAAGAGATCGTTCAGTTCTAGAGATTCTTTGTCTTCACTGTCGCGATCAACCAGTGATTCTTCCTCCATGAGATCATATTCTACTGATTCTTCTCTGTTATCACGGATTGCAAGTGATTCCCATGAAAAATCCGCGAGAATGTCGATCGAAGAAGAAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

346

Amino Acids

38.04

Weight (kDa)

9.53

Isoelectric Point (pI)

68.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 86
AccB1I GGYRCC 1 cut(s) 802
AccI GTMKAC 1 cut(s) 638
AccII CGCG 2 cut(s) 919, 1010
AciI CCGC 8 cut(s) 213, 237, 324, 657, 743, 800, 806, 1008
AclWI GGATC 1 cut(s) 239
AcsI RAATTY 1 cut(s) 1032
AcuI CTGAAG 1 cut(s) 560
AcyI GRCGYC 2 cut(s) 677, 803
AfaI GTAC 3 cut(s) 14, 593, 617
AfiI CCNNNNNNNGG 1 cut(s) 335
AgsI TTSAA 2 cut(s) 178, 352
AjnI CCWGG 3 cut(s) 287, 509, 629
AluBI AGCT 5 cut(s) 341, 457, 473, 490, 507
AluI AGCT 5 cut(s) 341, 457, 473, 490, 507
Alw26I GTCTC 1 cut(s) 684
AlwI GGATC 1 cut(s) 239
AlwNI CAGNNNCTG 1 cut(s) 538
Ama87I CYCGRG 1 cut(s) 495
AoxI GGCC 1 cut(s) 290
ApeKI GCWGC 2 cut(s) 835, 838
ApoI RAATTY 1 cut(s) 1032
AspLEI GCGC 2 cut(s) 805, 863
AsuHPI GGTGA 5 cut(s) 407, 484, 660, 661, 805
AvaI CYCGRG 1 cut(s) 495
BanI GGYRCC 1 cut(s) 802
BbsI GAAGAC 2 cut(s) 555, 900
BbvI GCAGC 2 cut(s) 822, 825
BccI CCATC 4 cut(s) 314, 406, 790, 842
BceAI ACGGC 1 cut(s) 690
BciT130I CCWGG 3 cut(s) 289, 511, 631
BcoDI GTCTC 1 cut(s) 684
BfaI CTAG 1 cut(s) 894
BfmI CTRYAG 1 cut(s) 584
BfoI RGCGCY 2 cut(s) 806, 864
BisI GCNGC 5 cut(s) 214, 801, 806, 836, 839
BlsI GCNGC 5 cut(s) 215, 802, 807, 837, 840
Bme1390I CCNGG 3 cut(s) 289, 511, 631
BmeT110I CYCGRG 1 cut(s) 495
BmiI GGNNCC 2 cut(s) 627, 804
BmrFI CCNGG 3 cut(s) 289, 511, 631
BmsI GCATC 1 cut(s) 678
BpiI GAAGAC 2 cut(s) 555, 900
BpmI CTGGAG 1 cut(s) 493
Bpu10I CCTNAGC 1 cut(s) 486
BpuEI CTTGAG 1 cut(s) 5
BsaHI GRCGYC 2 cut(s) 677, 803
BsaJI CCNNGG 3 cut(s) 621, 629, 789
BsaXI ACNNNNNCTCC 2 cut(s) 664, 694
Bsc4I CCNNNNNNNGG 1 cut(s) 335
Bse118I RCCGGY 3 cut(s) 47, 209, 808
Bse1I ACTGG 2 cut(s) 281, 927
Bse3DI GCAATG 2 cut(s) 69, 871
BseBI CCWGG 3 cut(s) 289, 511, 631
BseDI CCNNGG 3 cut(s) 621, 629, 789
BseGI GGATG 3 cut(s) 141, 165, 583
BseLI CCNNNNNNNGG 1 cut(s) 335
BseMI GCAATG 2 cut(s) 69, 871
BseMII CTCAG 2 cut(s) 311, 633
BseNI ACTGG 2 cut(s) 281, 927
BseRI GAGGAG 1 cut(s) 285
BseXI GCAGC 2 cut(s) 822, 825
BsgI GTGCAG 1 cut(s) 824
Bsh1236I CGCG 2 cut(s) 919, 1010
Bsh1285I CGRYCG 1 cut(s) 1023
BshFI GGCC 1 cut(s) 292
BshNI GGYRCC 1 cut(s) 802
BsiEI CGRYCG 1 cut(s) 1023
BsiHKCI CYCGRG 1 cut(s) 495
BsiSI CCGG 8 cut(s) 48, 210, 462, 545, 660, 683, 740, 809
BslFI GGGAC 3 cut(s) 286, 403, 836
BslI CCNNNNNNNGG 1 cut(s) 335
BsmAI GTCTC 1 cut(s) 684
BsmBI CGTCTC 1 cut(s) 684
BsmFI GGGAC 3 cut(s) 286, 403, 836
BsnI GGCC 1 cut(s) 292
BsoBI CYCGRG 1 cut(s) 495
Bsp143I GATC 6 cut(s) 244, 373, 881, 920, 947, 1020
Bsp68I TCGCGA 1 cut(s) 919
BspACI CCGC 8 cut(s) 213, 237, 324, 657, 743, 800, 806, 1008
BspANI GGCC 1 cut(s) 292
BspCNI CTCAG 2 cut(s) 310, 634
BspFNI CGCG 2 cut(s) 919, 1010
BspLI GGNNCC 2 cut(s) 627, 804
BspMAI CTGCAG 1 cut(s) 588
BspPI GGATC 1 cut(s) 239
BspQI GCTCTTC 1 cut(s) 770
BspT107I GGYRCC 1 cut(s) 802
BsrDI GCAATG 2 cut(s) 69, 871
BsrFI RCCGGY 3 cut(s) 47, 209, 808
BsrI ACTGG 2 cut(s) 281, 927
BssAI RCCGGY 3 cut(s) 47, 209, 808
BssECI CCNNGG 3 cut(s) 621, 629, 789
BssMI GATC 6 cut(s) 244, 373, 881, 920, 947, 1020
BssNI GRCGYC 2 cut(s) 677, 803
BssT1I CCWWGG 1 cut(s) 621
Bst2UI CCWGG 3 cut(s) 289, 511, 631
Bst4CI ACNGT 2 cut(s) 793, 915
Bst6I CTCTTC 3 cut(s) 87, 93, 770
BstACI GRCGYC 2 cut(s) 677, 803
BstC8I GCNNGC 3 cut(s) 211, 459, 657
BstDEI CTNAG 4 cut(s) 297, 486, 573, 642
BstDSI CCRYGG 1 cut(s) 789
BstF5I GGATG 3 cut(s) 141, 165, 583
BstFNI CGCG 2 cut(s) 919, 1010
BstH2I RGCGCY 2 cut(s) 806, 864
BstHHI GCGC 2 cut(s) 805, 863
BstKTI GATC 6 cut(s) 247, 376, 884, 923, 950, 1023
BstMAI GTCTC 1 cut(s) 684
BstMBI GATC 6 cut(s) 244, 373, 881, 920, 947, 1020
BstMCI CGRYCG 1 cut(s) 1023
BstMWI GCNNNNNNNGC 3 cut(s) 71, 683, 811
BstNI CCWGG 3 cut(s) 289, 511, 631
BstSCI CCNGG 3 cut(s) 287, 509, 629
BstSFI CTRYAG 1 cut(s) 584
BstUI CGCG 2 cut(s) 919, 1010
BstV1I GCAGC 2 cut(s) 822, 825
BstV2I GAAGAC 2 cut(s) 555, 900
BstXI CCANNNNNNTGG 1 cut(s) 796
BsuRI GGCC 1 cut(s) 292
BtgI CCRYGG 1 cut(s) 789
BtgZI GCGATG 2 cut(s) 292, 701
BtsCI GGATG 3 cut(s) 141, 165, 583
BtsIMutI CAGTG 2 cut(s) 911, 934
BtuMI TCGCGA 1 cut(s) 919
Cac8I GCNNGC 3 cut(s) 211, 459, 657
CaiI CAGNNNCTG 1 cut(s) 538
CfoI GCGC 2 cut(s) 805, 863
Cfr10I RCCGGY 3 cut(s) 47, 209, 808
CseI GACGC 1 cut(s) 666
Csp6I GTAC 3 cut(s) 13, 592, 616
CviAII CATG 2 cut(s) 943, 998
CviQI GTAC 3 cut(s) 13, 592, 616
DdeI CTNAG 4 cut(s) 297, 486, 573, 642
DinI GGCGCC 1 cut(s) 804
DpnI GATC 6 cut(s) 246, 375, 883, 922, 949, 1022
DpnII GATC 6 cut(s) 244, 373, 881, 920, 947, 1020
DraI TTTAAA 1 cut(s) 345
DrdI GACNNNNNNGTC 1 cut(s) 86
DseDI GACNNNNNNGTC 1 cut(s) 86
Eam1104I CTCTTC 3 cut(s) 87, 93, 770
EarI CTCTTC 3 cut(s) 87, 93, 770
EciI GGCGGA 1 cut(s) 313
Eco130I CCWWGG 1 cut(s) 621
Eco147I AGGCCT 1 cut(s) 292
Eco32I GATATC 1 cut(s) 139
Eco57I CTGAAG 1 cut(s) 560
Eco88I CYCGRG 1 cut(s) 495
EcoRII CCWGG 3 cut(s) 287, 509, 629
EcoRV GATATC 1 cut(s) 139
EcoT14I CCWWGG 1 cut(s) 621
EgeI GGCGCC 1 cut(s) 804
EheI GGCGCC 1 cut(s) 804
ErhI CCWWGG 1 cut(s) 621
Esp3I CGTCTC 1 cut(s) 684
FaeI CATG 2 cut(s) 946, 1001
FaiI YATR 4 cut(s) 704, 944, 952, 999
FaqI GGGAC 3 cut(s) 286, 403, 836
FatI CATG 2 cut(s) 942, 997
FauI CCCGC 2 cut(s) 664, 736
FblI GTMKAC 1 cut(s) 638
Fnu4HI GCNGC 5 cut(s) 214, 801, 806, 836, 839
FokI GGATG 3 cut(s) 128, 152, 590
Fsp4HI GCNGC 5 cut(s) 214, 801, 806, 836, 839
FspBI CTAG 1 cut(s) 894
GlaI GCGC 2 cut(s) 804, 862
GluI GCNGC 5 cut(s) 214, 801, 806, 836, 839
GsuI CTGGAG 1 cut(s) 493
HaeII RGCGCY 2 cut(s) 806, 864
HaeIII GGCC 1 cut(s) 292
HapII CCGG 8 cut(s) 48, 210, 462, 545, 660, 683, 740, 809
HgaI GACGC 1 cut(s) 666
HhaI GCGC 2 cut(s) 805, 863
Hin1I GRCGYC 2 cut(s) 677, 803
Hin1II CATG 2 cut(s) 946, 1001
Hin6I GCGC 2 cut(s) 803, 861
HinP1I GCGC 2 cut(s) 803, 861
HpaII CCGG 8 cut(s) 48, 210, 462, 545, 660, 683, 740, 809
HphI GGTGA 5 cut(s) 407, 484, 660, 661, 805
Hpy166II GTNNAC 2 cut(s) 152, 639
Hpy188I TCNGA 6 cut(s) 199, 244, 338, 403, 540, 858
Hpy188III TCNNGA 3 cut(s) 28, 894, 918
Hpy8I GTNNAC 2 cut(s) 152, 639
Hpy99I CGWCG 1 cut(s) 676
HpyAV CCTTC 2 cut(s) 640, 712
HpyCH4III ACNGT 2 cut(s) 793, 915
HpyCH4V TGCA 7 cut(s) 74, 532, 586, 814, 841, 876, 986
HpyF10VI GCNNNNNNNGC 3 cut(s) 71, 683, 811
HpyF3I CTNAG 4 cut(s) 297, 486, 573, 642
Hsp92I GRCGYC 2 cut(s) 677, 803
Hsp92II CATG 2 cut(s) 946, 1001
HspAI GCGC 2 cut(s) 803, 861
KasI GGCGCC 1 cut(s) 802
KroI GCCGGC 1 cut(s) 209
KroNI GCCGGC 1 cut(s) 211
Kzo9I GATC 6 cut(s) 244, 373, 881, 920, 947, 1020
LguI GCTCTTC 1 cut(s) 770
LmnI GCTCC 2 cut(s) 338, 512
Lsp1109I GCAGC 2 cut(s) 822, 825
LweI GCATC 1 cut(s) 678
MaeI CTAG 1 cut(s) 894
MalI GATC 6 cut(s) 246, 375, 883, 922, 949, 1022
MboI GATC 6 cut(s) 244, 373, 881, 920, 947, 1020
MluCI AATT 4 cut(s) 125, 444, 767, 1032
Mly113I GGCGCC 1 cut(s) 803
MlyI GAGTC 3 cut(s) 407, 425, 545
MmeI TCCRAC 2 cut(s) 222, 381
MroNI GCCGGC 1 cut(s) 209
MseI TTAA 4 cut(s) 128, 318, 344, 597
MslI CAYNNNNRTG 2 cut(s) 794, 846
MspI CCGG 8 cut(s) 48, 210, 462, 545, 660, 683, 740, 809
MspR9I CCNGG 3 cut(s) 289, 511, 631
MvaI CCWGG 3 cut(s) 289, 511, 631
MvnI CGCG 2 cut(s) 919, 1010
MwoI GCNNNNNNNGC 3 cut(s) 71, 683, 811
NaeI GCCGGC 1 cut(s) 211
NarI GGCGCC 1 cut(s) 803
NdeII GATC 6 cut(s) 244, 373, 881, 920, 947, 1020
NgoMIV GCCGGC 1 cut(s) 209
NlaIII CATG 2 cut(s) 946, 1001
NlaIV GGNNCC 2 cut(s) 627, 804
NruI TCGCGA 1 cut(s) 919
PceI AGGCCT 1 cut(s) 292
PciSI GCTCTTC 1 cut(s) 770
PcsI WCGNNNNNNNCGW 1 cut(s) 407
PdiI GCCGGC 1 cut(s) 211
PfeI GAWTC 9 cut(s) 107, 535, 665, 827, 853, 899, 932, 962, 992
PflFI GACNNNGTC 1 cut(s) 676
PfoI TCCNGGA 1 cut(s) 509
PkrI GCNGC 5 cut(s) 215, 802, 807, 837, 840
Ple19I CGATCG 1 cut(s) 1023
PleI GAGTC 3 cut(s) 406, 424, 545
PluTI GGCGCC 1 cut(s) 806
PpsI GAGTC 3 cut(s) 406, 424, 545
Psp6I CCWGG 3 cut(s) 287, 509, 629
PspGI CCWGG 3 cut(s) 287, 509, 629
PspN4I GGNNCC 2 cut(s) 627, 804
PstI CTGCAG 1 cut(s) 588
PstNI CAGNNNCTG 1 cut(s) 538
PsyI GACNNNGTC 1 cut(s) 676
PvuI CGATCG 1 cut(s) 1023
RruI TCGCGA 1 cut(s) 919
RsaI GTAC 3 cut(s) 14, 593, 617
RsaNI GTAC 3 cut(s) 13, 592, 616
RseI CAYNNNNRTG 2 cut(s) 794, 846
SapI GCTCTTC 1 cut(s) 770
SaqAI TTAA 4 cut(s) 128, 318, 344, 597
SatI GCNGC 5 cut(s) 214, 801, 806, 836, 839
Sau3AI GATC 6 cut(s) 244, 373, 881, 920, 947, 1020
SchI GAGTC 3 cut(s) 407, 425, 545
ScrFI CCNGG 3 cut(s) 289, 511, 631
SfaNI GCATC 1 cut(s) 678
SfcI CTRYAG 1 cut(s) 584
SfoI GGCGCC 1 cut(s) 804
SgrAI CRCCGGYG 1 cut(s) 808
SmiMI CAYNNNNRTG 2 cut(s) 794, 846
SmlI CTYRAG 1 cut(s) 20
SmoI CTYRAG 1 cut(s) 20
Sse9I AATT 4 cut(s) 125, 444, 767, 1032
SseBI AGGCCT 1 cut(s) 292
SsiI CCGC 8 cut(s) 213, 237, 324, 657, 743, 800, 806, 1008
SspDI GGCGCC 1 cut(s) 802
SspMI CTAG 1 cut(s) 894
StuI AGGCCT 1 cut(s) 292
StyD4I CCNGG 3 cut(s) 287, 509, 629
StyI CCWWGG 1 cut(s) 621
TaaI ACNGT 2 cut(s) 793, 915
TaqI TCGA 7 cut(s) 90, 117, 171, 410, 554, 1019, 1023
TasI AATT 4 cut(s) 125, 444, 767, 1032
TatI WGTACW 2 cut(s) 12, 615
TauI GCSGC 3 cut(s) 216, 803, 808
TfiI GAWTC 9 cut(s) 107, 535, 665, 827, 853, 899, 932, 962, 992
Tru1I TTAA 4 cut(s) 128, 318, 344, 597
Tru9I TTAA 4 cut(s) 128, 318, 344, 597
TscAI CASTG 2 cut(s) 918, 934
TseI GCWGC 2 cut(s) 835, 838
TspDTI ATGAA 1 cut(s) 1014
TspGWI ACGGA 3 cut(s) 534, 832, 994
TspRI CASTG 2 cut(s) 918, 934
Tth111I GACNNNGTC 1 cut(s) 676
XapI RAATTY 1 cut(s) 1032
XbaI TCTAGA 1 cut(s) 893
XmiI GTMKAC 1 cut(s) 638
XspI CTAG 1 cut(s) 894
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.