Rh1AG434600

membrane-associated kinase regulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0009504
Physical Location & Seq
Reverse (-)
66004974 .. 66006220
1247 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG434600.1

Sequence Viewer

Length: 1035 bp
ATGGAGACTCTGTACTTTCTTAAGTTCTGGAAACCCAATCCTTCTCCCATTGCAATTCCCAGTCCTTTGGTAGAAACAGACAATGAATTTGAGGTGGAAGAGGAGGAAGAAGAAGACTCGTTCTTCGATTTGGAGATTAGTACAGAATCAAACACCAAAACCCATGAAGAAAGTTCCAAGCTTTACACAAAAGAAGCCAAAACAGAACCAACCCTTTCTGTTCCTCCTCCTTCTGATCCAATTTCCAAAAGGAAAATCCTCCCAATTGAACCCATTTCCAAGCCTCAGTCCCCCATTGCTCTACTCAAATCAGCTCCAAAGTTTGGGGTTTCGATTTTCAAAAAACCCAAATCAGCTTCAACTAAGAAAACAGAGAAAACAGGGGAAACAGAGTCCAGCATTTTGATGGAGACCCAGAAGCAGAAAAAGCCAGAAGCCAAAGGCAATGAAGCTTCAAGCCCTTCTAAGCTGACCAGGGAAAACAGTTCGAGAAAAACTGGAAAGATATTGCAGAGCCAGAGCTCCGATGATTCGAAAACAGAGCGGTTTTCAAAGGATGTGATAAACAAGTACCTGAAGCTAATCAAGCCTCTGTACTTGAAAGTTTCCAAGAGGTACGGTGACAAGGTGAAGGTTTCCGGCGACTTGCCGATGGCATCGCCGACGTCGTCGCCGGCGGTGTCTATGTACTCCCCGAGGAAGGAGAGGCAGGGGAATATTCCTGCCGGGATTCGAGTTGTTTGTAAGCATCTTGGGAAGAGCAAGTCGGCCACGGTGGCGATGGCGGTGGCGCCGCCGGTGAACCGGAGAGACGACTCGCTGTTGCTTCAAGCAGATGGGATTCAGAGCGCCATTCTGCATTGCAAGAGATCATTCAATTCTAGAGATTCTTCTTGCCTATCAAGATCAACAAGTTGGACTAGTTCGTCCATGAACTCATATTCTAGTGATTCTTCTATGCTATCACGGTTTGCGATCGAATCCCATGAGAGATCTGCGAGAAGCTCCATAGAGGAAGAGCTAGCATGCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

344

Amino Acids

38.15

Weight (kDa)

9.16

Isoelectric Point (pI)

75.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 925
AatII GACGTC 1 cut(s) 668
AccB1I GGYRCC 1 cut(s) 790
AccB7I CCANNNNNTGG 1 cut(s) 323
AccBSI CCGCTC 1 cut(s) 544
AciI CCGC 4 cut(s) 544, 677, 785, 794
AclWI GGATC 1 cut(s) 230
AcoI YGGCCR 1 cut(s) 768
AcsI RAATTY 1 cut(s) 86
AcuI CTGAAG 1 cut(s) 596
AcyI GRCGYC 2 cut(s) 665, 791
AfaI GTAC 6 cut(s) 14, 142, 572, 596, 617, 689
AfiI CCNNNNNNNGG 2 cut(s) 323, 700
AflII CTTAAG 1 cut(s) 20
AgsI TTSAA 8 cut(s) 269, 340, 360, 456, 552, 601, 830, 877
AhlI ACTAGT 1 cut(s) 920
AjnI CCWGG 1 cut(s) 473
AluBI AGCT 9 cut(s) 181, 314, 356, 452, 469, 522, 580, 1005, 1021
AluI AGCT 9 cut(s) 181, 314, 356, 452, 469, 522, 580, 1005, 1021
Alw21I GWGCWC 1 cut(s) 524
Alw26I GTCTC 2 cut(s) 404, 804
AlwI GGATC 1 cut(s) 230
Ama87I CYCGRG 1 cut(s) 694
AoxI GGCC 1 cut(s) 768
ApoI RAATTY 1 cut(s) 86
ArsI GACNNNNNNTTYG 4 cut(s) 71, 103, 107, 139
AspLEI GCGC 2 cut(s) 793, 851
AsuC2I CCSGG 1 cut(s) 727
AsuHPI GGTGA 3 cut(s) 632, 640, 811
AsuII TTCGAA 1 cut(s) 533
AsuNHI GCTAGC 1 cut(s) 1021
AvaI CYCGRG 1 cut(s) 694
BanI GGYRCC 1 cut(s) 790
BanII GRGCYC 1 cut(s) 524
BbsI GAAGAC 1 cut(s) 120
Bbv12I GWGCWC 1 cut(s) 524
BccI CCATC 4 cut(s) 400, 646, 775, 830
BciT130I CCWGG 1 cut(s) 475
BcnI CCSGG 1 cut(s) 727
BcoDI GTCTC 2 cut(s) 404, 804
BcuI ACTAGT 1 cut(s) 920
BfaI CTAG 4 cut(s) 882, 921, 945, 1022
BfoI RGCGCY 2 cut(s) 794, 852
BfrI CTTAAG 1 cut(s) 20
BglI GCCNNNNNGGC 1 cut(s) 776
BglII AGATCT 1 cut(s) 992
BisI GCNGC 1 cut(s) 794
BlsI GCNGC 1 cut(s) 795
Bme1390I CCNGG 2 cut(s) 475, 727
BmeT110I CYCGRG 1 cut(s) 694
BmiI GGNNCC 1 cut(s) 792
BmrFI CCNGG 2 cut(s) 475, 727
BmrI ACTGGG 1 cut(s) 54
BmsI GCATC 2 cut(s) 665, 757
BmtI GCTAGC 1 cut(s) 1025
BmuI ACTGGG 1 cut(s) 54
BpiI GAAGAC 1 cut(s) 120
Bpu14I TTCGAA 1 cut(s) 533
BpuMI CCSGG 1 cut(s) 727
BsaHI GRCGYC 2 cut(s) 665, 791
BsaI GGTCTC 1 cut(s) 404
BsaJI CCNNGG 3 cut(s) 474, 695, 771
BsaWI WCCGGW 1 cut(s) 804
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 2 cut(s) 323, 700
Bse118I RCCGGY 2 cut(s) 673, 796
Bse1I ACTGG 2 cut(s) 60, 502
Bse3DI GCAATG 4 cut(s) 48, 294, 451, 859
BseBI CCWGG 1 cut(s) 475
BseDI CCNNGG 3 cut(s) 474, 695, 771
BseGI GGATG 1 cut(s) 562
BseLI CCNNNNNNNGG 2 cut(s) 323, 700
BseMI GCAATG 4 cut(s) 48, 294, 451, 859
BseMII CTCAG 1 cut(s) 299
BseNI ACTGG 2 cut(s) 60, 502
BseRI GAGGAG 2 cut(s) 116, 216
Bsh1285I CGRYCG 1 cut(s) 978
BshFI GGCC 1 cut(s) 770
BshNI GGYRCC 1 cut(s) 790
BsiEI CGRYCG 1 cut(s) 978
BsiHKAI GWGCWC 1 cut(s) 524
BsiHKCI CYCGRG 1 cut(s) 694
BsiSI CCGG 5 cut(s) 639, 674, 726, 797, 805
BslFI GGGAC 1 cut(s) 274
BslI CCNNNNNNNGG 2 cut(s) 323, 700
BsmAI GTCTC 2 cut(s) 404, 804
BsmBI CGTCTC 1 cut(s) 804
BsmFI GGGAC 1 cut(s) 274
BsnI GGCC 1 cut(s) 770
Bso31I GGTCTC 1 cut(s) 404
BsoBI CYCGRG 1 cut(s) 694
Bsp119I TTCGAA 1 cut(s) 533
Bsp1286I GDGCHC 1 cut(s) 524
Bsp143I GATC 5 cut(s) 235, 869, 905, 975, 992
BspACI CCGC 4 cut(s) 544, 677, 785, 794
BspANI GGCC 1 cut(s) 770
BspCNI CTCAG 1 cut(s) 298
BspLI GGNNCC 1 cut(s) 792
BspOI GCTAGC 1 cut(s) 1025
BspPI GGATC 1 cut(s) 230
BspQI GCTCTTC 2 cut(s) 752, 1011
BspT104I TTCGAA 1 cut(s) 533
BspT107I GGYRCC 1 cut(s) 790
BspTI CTTAAG 1 cut(s) 20
BspTNI GGTCTC 1 cut(s) 404
BsrBI CCGCTC 1 cut(s) 544
BsrDI GCAATG 4 cut(s) 48, 294, 451, 859
BsrFI RCCGGY 2 cut(s) 673, 796
BsrI ACTGG 2 cut(s) 60, 502
BssAI RCCGGY 2 cut(s) 673, 796
BssECI CCNNGG 3 cut(s) 474, 695, 771
BssMI GATC 5 cut(s) 235, 869, 905, 975, 992
BssNI GRCGYC 2 cut(s) 665, 791
Bst2UI CCWGG 1 cut(s) 475
Bst4CI ACNGT 4 cut(s) 485, 620, 775, 969
Bst6I CTCTTC 3 cut(s) 93, 752, 1011
BstACI GRCGYC 2 cut(s) 665, 791
BstAFI CTTAAG 1 cut(s) 20
BstBI TTCGAA 1 cut(s) 533
BstC8I GCNNGC 3 cut(s) 675, 1023, 1027
BstDEI CTNAG 3 cut(s) 285, 363, 465
BstDSI CCRYGG 1 cut(s) 771
BstF5I GGATG 1 cut(s) 562
BstH2I RGCGCY 2 cut(s) 794, 852
BstHHI GCGC 2 cut(s) 793, 851
BstKTI GATC 5 cut(s) 238, 872, 908, 978, 995
BstMAI GTCTC 2 cut(s) 404, 804
BstMBI GATC 5 cut(s) 235, 869, 905, 975, 992
BstMCI CGRYCG 1 cut(s) 978
BstMWI GCNNNNNNNGC 3 cut(s) 427, 586, 776
BstNI CCWGG 1 cut(s) 475
BstNSI RCATGY 1 cut(s) 1029
BstSCI CCNGG 2 cut(s) 473, 725
BstV2I GAAGAC 1 cut(s) 120
BstX2I RGATCY 1 cut(s) 992
BstXI CCANNNNNNTGG 1 cut(s) 67
BstYI RGATCY 1 cut(s) 992
BsuRI GGCC 1 cut(s) 770
BtgI CCRYGG 1 cut(s) 771
BtgZI GCGATG 2 cut(s) 642, 794
BtsCI GGATG 1 cut(s) 562
Cac8I GCNNGC 3 cut(s) 675, 1023, 1027
CfoI GCGC 2 cut(s) 793, 851
Cfr10I RCCGGY 2 cut(s) 673, 796
Csp6I GTAC 6 cut(s) 13, 141, 571, 595, 616, 688
CviAII CATG 4 cut(s) 164, 931, 986, 1026
CviQI GTAC 6 cut(s) 13, 141, 571, 595, 616, 688
DdeI CTNAG 3 cut(s) 285, 363, 465
DinI GGCGCC 1 cut(s) 792
DpnI GATC 5 cut(s) 237, 871, 907, 977, 994
DpnII GATC 5 cut(s) 235, 869, 905, 975, 992
DrdI GACNNNNNNGTC 1 cut(s) 925
DseDI GACNNNNNNGTC 1 cut(s) 925
EaeI YGGCCR 1 cut(s) 768
Eam1104I CTCTTC 3 cut(s) 93, 752, 1011
EarI CTCTTC 3 cut(s) 93, 752, 1011
Ecl136II GAGCTC 1 cut(s) 522
Eco24I GRGCYC 1 cut(s) 524
Eco31I GGTCTC 1 cut(s) 404
Eco53kI GAGCTC 1 cut(s) 522
Eco57I CTGAAG 1 cut(s) 596
Eco88I CYCGRG 1 cut(s) 694
EcoICRI GAGCTC 1 cut(s) 522
EcoRII CCWGG 1 cut(s) 473
EcoT38I GRGCYC 1 cut(s) 524
EgeI GGCGCC 1 cut(s) 792
EheI GGCGCC 1 cut(s) 792
Esp3I CGTCTC 1 cut(s) 804
FaeI CATG 4 cut(s) 167, 934, 989, 1029
FaiI YATR 8 cut(s) 165, 686, 932, 940, 959, 987, 1010, 1027
FaqI GGGAC 1 cut(s) 274
FatI CATG 4 cut(s) 163, 930, 985, 1025
Fnu4HI GCNGC 1 cut(s) 794
FokI GGATG 1 cut(s) 569
FriOI GRGCYC 1 cut(s) 524
Fsp4HI GCNGC 1 cut(s) 794
FspBI CTAG 4 cut(s) 882, 921, 945, 1022
GlaI GCGC 2 cut(s) 792, 850
GluI GCNGC 1 cut(s) 794
HaeII RGCGCY 2 cut(s) 794, 852
HaeIII GGCC 1 cut(s) 770
HapII CCGG 5 cut(s) 639, 674, 726, 797, 805
HhaI GCGC 2 cut(s) 793, 851
Hin1I GRCGYC 2 cut(s) 665, 791
Hin1II CATG 4 cut(s) 167, 934, 989, 1029
Hin6I GCGC 2 cut(s) 791, 849
HinP1I GCGC 2 cut(s) 791, 849
HindIII AAGCTT 2 cut(s) 179, 450
HpaII CCGG 5 cut(s) 639, 674, 726, 797, 805
HphI GGTGA 3 cut(s) 632, 640, 811
Hpy166II GTNNAC 1 cut(s) 802
Hpy188I TCNGA 4 cut(s) 235, 526, 846, 1034
Hpy188III TCNNGA 4 cut(s) 28, 489, 882, 903
Hpy8I GTNNAC 1 cut(s) 802
Hpy99I CGWCG 3 cut(s) 667, 670, 673
HpyAV CCTTC 5 cut(s) 51, 240, 471, 625, 694
HpyCH4III ACNGT 4 cut(s) 485, 620, 775, 969
HpyCH4IV ACGT 1 cut(s) 665
HpyCH4V TGCA 4 cut(s) 53, 511, 859, 864
HpyF10VI GCNNNNNNNGC 3 cut(s) 427, 586, 776
HpyF3I CTNAG 3 cut(s) 285, 363, 465
HpySE526I ACGT 1 cut(s) 665
Hsp92I GRCGYC 2 cut(s) 665, 791
Hsp92II CATG 4 cut(s) 167, 934, 989, 1029
HspAI GCGC 2 cut(s) 791, 849
KasI GGCGCC 1 cut(s) 790
KroI GCCGGC 1 cut(s) 673
KroNI GCCGGC 1 cut(s) 675
Kzo9I GATC 5 cut(s) 235, 869, 905, 975, 992
LguI GCTCTTC 2 cut(s) 752, 1011
LmnI GCTCC 3 cut(s) 319, 527, 1010
LweI GCATC 2 cut(s) 665, 757
MaeI CTAG 4 cut(s) 882, 921, 945, 1022
MaeII ACGT 1 cut(s) 665
MaeIII GTNAC 1 cut(s) 620
MalI GATC 5 cut(s) 237, 871, 907, 977, 994
MbiI CCGCTC 1 cut(s) 544
MboI GATC 5 cut(s) 235, 869, 905, 975, 992
MfeI CAATTG 1 cut(s) 264
MflI RGATCY 1 cut(s) 992
MhlI GDGCHC 1 cut(s) 524
MluCI AATT 5 cut(s) 54, 86, 240, 264, 877
Mly113I GGCGCC 1 cut(s) 791
MlyI GAGTC 3 cut(s) 110, 401, 809
MmeI TCCRAC 1 cut(s) 896
MreI CGCCGGCG 1 cut(s) 673
MroNI GCCGGC 1 cut(s) 673
MseI TTAA 1 cut(s) 21
MslI CAYNNNNRTG 1 cut(s) 404
MspCI CTTAAG 1 cut(s) 20
MspI CCGG 5 cut(s) 639, 674, 726, 797, 805
MspR9I CCNGG 2 cut(s) 475, 727
MunI CAATTG 1 cut(s) 264
MvaI CCWGG 1 cut(s) 475
MwoI GCNNNNNNNGC 3 cut(s) 427, 586, 776
NaeI GCCGGC 1 cut(s) 675
NarI GGCGCC 1 cut(s) 791
NciI CCSGG 1 cut(s) 727
NdeII GATC 5 cut(s) 235, 869, 905, 975, 992
NgoMIV GCCGGC 1 cut(s) 673
NheI GCTAGC 1 cut(s) 1021
NlaIII CATG 4 cut(s) 167, 934, 989, 1029
NlaIV GGNNCC 1 cut(s) 792
NmuCI GTSAC 1 cut(s) 620
NspI RCATGY 1 cut(s) 1029
NspV TTCGAA 1 cut(s) 533
PaeI GCATGC 1 cut(s) 1029
PciSI GCTCTTC 2 cut(s) 752, 1011
PcsI WCGNNNNNNNCGW 1 cut(s) 665
PdiI GCCGGC 1 cut(s) 675
PfeI GAWTC 7 cut(s) 146, 530, 730, 841, 887, 950, 980
PflFI GACNNNGTC 1 cut(s) 667
PflMI CCANNNNNTGG 1 cut(s) 323
PkrI GCNGC 1 cut(s) 795
Ple19I CGATCG 1 cut(s) 978
PleI GAGTC 3 cut(s) 110, 400, 809
PluTI GGCGCC 1 cut(s) 794
PpsI GAGTC 3 cut(s) 110, 400, 809
Psp124BI GAGCTC 1 cut(s) 524
Psp6I CCWGG 1 cut(s) 473
PspGI CCWGG 1 cut(s) 473
PspN4I GGNNCC 1 cut(s) 792
PsuI RGATCY 1 cut(s) 992
PsyI GACNNNGTC 1 cut(s) 667
PvuI CGATCG 1 cut(s) 978
RsaI GTAC 6 cut(s) 14, 142, 572, 596, 617, 689
RsaNI GTAC 6 cut(s) 13, 141, 571, 595, 616, 688
RseI CAYNNNNRTG 1 cut(s) 404
SacI GAGCTC 1 cut(s) 524
SapI GCTCTTC 2 cut(s) 752, 1011
SaqAI TTAA 1 cut(s) 21
SatI GCNGC 1 cut(s) 794
Sau3AI GATC 5 cut(s) 235, 869, 905, 975, 992
SchI GAGTC 3 cut(s) 110, 401, 809
ScrFI CCNGG 2 cut(s) 475, 727
SduI GDGCHC 1 cut(s) 524
SfaNI GCATC 2 cut(s) 665, 757
SfoI GGCGCC 1 cut(s) 792
SfuI TTCGAA 1 cut(s) 533
SgrAI CRCCGGYG 2 cut(s) 673, 796
SmiMI CAYNNNNRTG 1 cut(s) 404
SmlI CTYRAG 1 cut(s) 20
SmoI CTYRAG 1 cut(s) 20
SpeI ACTAGT 1 cut(s) 920
SphI GCATGC 1 cut(s) 1029
Sse9I AATT 5 cut(s) 54, 86, 240, 264, 877
SsiI CCGC 4 cut(s) 544, 677, 785, 794
SspDI GGCGCC 1 cut(s) 790
SspI AATATT 1 cut(s) 718
SspMI CTAG 4 cut(s) 882, 921, 945, 1022
SstI GAGCTC 1 cut(s) 524
StyD4I CCNGG 2 cut(s) 473, 725
TaaI ACNGT 4 cut(s) 485, 620, 775, 969
TaiI ACGT 1 cut(s) 668
TaqI TCGA 6 cut(s) 126, 332, 488, 533, 733, 978
TasI AATT 5 cut(s) 54, 86, 240, 264, 877
TatI WGTACW 4 cut(s) 12, 140, 594, 687
TauI GCSGC 1 cut(s) 796
TfiI GAWTC 7 cut(s) 146, 530, 730, 841, 887, 950, 980
Tru1I TTAA 1 cut(s) 21
Tru9I TTAA 1 cut(s) 21
TseFI GTSAC 1 cut(s) 620
Tsp45I GTSAC 1 cut(s) 620
TspDTI ATGAA 4 cut(s) 99, 180, 462, 947
Tth111I GACNNNGTC 1 cut(s) 667
Van91I CCANNNNNTGG 1 cut(s) 323
Vha464I CTTAAG 1 cut(s) 20
XapI RAATTY 1 cut(s) 86
XbaI TCTAGA 1 cut(s) 881
XceI RCATGY 1 cut(s) 1029
XcmI CCANNNNNNNNNTGG 2 cut(s) 403, 778
XspI CTAG 4 cut(s) 882, 921, 945, 1022
ZraI GACGTC 1 cut(s) 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.