Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0007142

Physical Location & Seq

Genomic Coordinates Reverse (-)

34969282 .. 34971213

Gene Structure 1932 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1288700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1932 bp

ATGGCTCTCTCCAAACCCACCTTCCTTGCCCACCTCAAAACCCTAGCAAAACCCCCAATCCGCCGCCGTTTAACGCCACCCTCCGTCATCTCCCTCCGCTTCCTCTCCTTCGCCACCCCCGAAGAAGCCGCGGCCGAACGACGCCAGCGGAAGCGCCGCCTCCGACTCGAACCCCCGCTCTCCTCCCTCCACCGCAACCAGCAACAACAACAGCAACAGTCCCCAAAACCCCAAATAAACCCTAACGCCCCGAAGCTCCCCGAACCCGTCTCGGCCCTCTCCGGCAACCGCCTCAACCTCCACAACCGCATTCTCACCCTCATCCGCCAGAATGATCTCGAGGAGGCAGCACTCTACACTCGCCACTCCATCTACTCCAACTGCCGACCCACCATTTTCACCGTCAATGCCGTGCTCACCGCTCAGCTCCGCCAGTCCAAGTACTCCGACCTCCTCACCCTCCACCGCTTCATCACTCAGGCCGGGGTTGCCCCCAATATCATCACTCACAATCTCATCTTCCAGACTTATCTGGATTGCCGAAAGCCCGATACCGCCATGGAACACTACAAGCAGCTGATCAATGACGCCCCTTTCAACCCTTCCCCAACCACTTATCGGATTTTGATCAAAGGGCTAGTGGATAACAACAAATTGGACAGGGCTATGGAGCTCAAGGAGGAAATTGATGTCAAGGGTTTCGCCCCGGACCCGGTTGTTTACCATTATTTGATGCAGGGTTGTGTAAAGAATTCGGATTCGGATGGGGTTTTCAAGCTTTTCGAGGAGCTGAAGGAGAAGTTGGGAGGGGTTGTGGAAGATGGGGTTGTGTATGGGAACTTGATGAAGGGGTATTTCATGAGGGAGATGGAGAAGGAGGCCATGGAGTGTTATGAGGAGGCTGTGGGGGAGAGTTCCAAGGTGAAGATGAGCGCGGTGGCTTATAATTCGGTTTTGGATGCATTGAGCAAGAATGGCAAGTTTGATGAGGCCTTGAGGTTGTTTGATAGGATGATCCAGGAGCATAACCCACCGAGGCGCTTGGCTGTGAATTTGGGAAGCTTTAATGTGATGGTGGACGGGTATTGCGCGCAAGGGAGGTTTAAGGATGCCATTGAGGTTTTCAGGAAGATGGGAGACTATAGGTGTAGTCCGGATACATTGTCTTTCAATAATTTGATTGATCAGTTGTGTAAGAATGGGATGCTGAGTGAAGCTGAGGAGCTTTATGGAGAAATGCCCGAGAAGGGAGTGAATCCGGATGAGTTTACATATGTCCTGTTGATGGATACTTGTTTTGAGGAGAATAGGCCAGATGATGCAGCGGAGTACTTCAGAAAAATGGTTGATGCCAAGCTGAGGCCGAACTTGGCAGTTTATAATAAGTTGGCTGACGGATTGGTTAAGGTAGGGAAAATTGATGAGGCAAAATTGTTTTTCGATTTGATGGTGAAGAAGCTCAAGATGGACATTCCGAGCTATCAGTTTATAATGAAGATGTTGAGTGATGCTGGGAAACTCGATGAGGTGTTTAATGTGGTTGATACAATGCTGGATGACGATGGGGTTGAATTCGATGAGGAGGTGCATGAGTTTGTTAAAGGGTTGCTGAGAAAGGAAGGGAGAGAGGACGAAGTTGGGAAGCTTATGGAGGAGAAAGAAAGGCAGAAGGCTGAAGCTAAGGCTAAGGAGCTGGAGGCAGCAGAAGCAGCAAAGAGAAGTGCAAGAGCTGCTGTCGCCTCCTTACTTCCATCCAAGTTGTTCGGGAATAAAGAACCCGAGACAGAGTCTGCAGAGGCCACAGAAAATGCAAGTGAGGCTGCCTCGAGTGAAGCTGCACAGACTGGTCAGGAGGAACATAGTGGAGAAGGCGAAAATTCAGCCGAGACAGAATCCAAAAGTGACGGTGCAACTGAGCAGGTATCCGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000166 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006349 GO:0006725 GO:0006807 GO:0007275 GO:0008144 GO:0008150 GO:0008152 GO:0009451 GO:0009507 GO:0009536 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0010468 GO:0016070 GO:0016787 GO:0016788 GO:0017076 GO:0019222 GO:0022414 GO:0030554 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0034641 GO:0035639 GO:0036094 GO:0040029 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0060255 GO:0061458 GO:0065007 GO:0071514 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:0097367 GO:1901265 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

644

Amino Acids

72.47

Weight (kDa)

5.44

Isoelectric Point (pI)

43.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	202 - 250	7.4e-07	PPR repeat family
PPR_1	PF12854	309 - 338	6.6e-06	PPR repeat
PPR_2	PF13041	311 - 344	5.8e-07	PPR repeat family
PPR	PF01535	313 - 340	3.1e-08	PPR repeat
PPR_1	PF12854	351 - 378	5.4e-07	PPR repeat
PPR_2	PF13041	353 - 394	1.7e-09	PPR repeat family
PPR	PF01535	354 - 381	2.7e-06	PPR repeat
PPR_1	PF12854	383 - 413	1.4e-08	PPR repeat
PPR_2	PF13041	385 - 432	2.1e-14	PPR repeat family
PPR	PF01535	389 - 418	3.3e-08	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0007142)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G49240
fragaria_vesca	FvH4_2g09580
malus_domestica	MD07G1288700.v1.1
prunus_persica	Prupe.2G310200_v2.0.a1
pyrus_communis	pycom01g22880
rosa_chinensis	RchiOBHm_Chr1g0380631
rosa_laevigata	RLG00000026280
rosa_multiflora	Rmu_sc0004647.1_g000010 Rmu_sc0020141.1_g000001 Rmu_sc0020141.1_g000002 Rmu_sc0021483.1_g000003 Rmu_ssc0000015.1_g000013
rosa_roxburghii	Rroxscaffold_4G00279120
rosa_rugosa	Rorug01G0122900 Rorug01G0423100
rosa_samantha	Rh1AG447400 Rh1BG113000 Rh1BG151000 Rh1BG402900 Rh1CG417300 Rh1DG182800 Rh1DG433200 Rh7CG247500
rosa_wichuraiana	Rw1G038940 Rw7G019950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	945, 1380, 1490
Acc36I	ACCTGC	1 cut(s)	1909
AccBSI	CCGCTC	2 cut(s)	178, 422
AccII	CGCG	3 cut(s)	131, 935, 1091
AccIII	TCCGGA	2 cut(s)	1153, 1258
AclWI	GGATC	1 cut(s)	1009
AcoI	YGGCCR	1 cut(s)	132
AcsI	RAATTY	4 cut(s)	751, 1051, 1571, 1876
AcuI	CTGAAG	3 cut(s)	812, 1318, 1695
AcyI	GRCGYC	2 cut(s)	142, 588
AfaI	GTAC	2 cut(s)	443, 1331
AfiI	CCNNNNNNNGG	5 cut(s)	618, 712, 1247, 1285, 1359
AgsI	TTSAA	4 cut(s)	598, 775, 1171, 1571
AjnI	CCWGG	1 cut(s)	1017
AjuI	GAANNNNNNNTTGG	3 cut(s)	37, 785, 817
Alw21I	GWGCWC	2 cut(s)	417, 675
Alw26I	GTCTC	4 cut(s)	274, 1131, 1775, 1880
AlwI	GGATC	1 cut(s)	1009
AlwNI	CAGNNNCTG	1 cut(s)	1790
Ama87I	CYCGRG	4 cut(s)	338, 1241, 1778, 1825
Aor13HI	TCCGGA	2 cut(s)	1153, 1258
AoxI	GGCC	8 cut(s)	132, 273, 480, 879, 990, 1310, 1361, 1797
ApeKI	GCWGC	8 cut(s)	347, 574, 1322, 1700, 1709, 1730, 1820, 1835
ApoI	RAATTY	4 cut(s)	751, 1051, 1571, 1876
AspLEI	GCGC	5 cut(s)	156, 935, 1041, 1091, 1093
AspS9I	GGNCC	2 cut(s)	274, 709
AsuC2I	CCSGG	3 cut(s)	484, 707, 713
AsuHPI	GGTGA	6 cut(s)	307, 391, 409, 448, 934, 1462
AvaI	CYCGRG	4 cut(s)	338, 1241, 1778, 1825
AvaII	GGWCC	1 cut(s)	709
BanII	GRGCYC	1 cut(s)	675
Bbv12I	GWGCWC	2 cut(s)	417, 675
BbvCI	CCTCAGC	2 cut(s)	1218, 1358
BbvI	GCAGC	8 cut(s)	359, 586, 1334, 1712, 1717, 1721, 1807, 1822
BceAI	ACGGC	2 cut(s)	51, 395
BciT130I	CCWGG	1 cut(s)	1019
BciVI	GTATCC	2 cut(s)	1150, 1282
BclI	TGATCA	3 cut(s)	579, 627, 1183
BcnI	CCSGG	3 cut(s)	484, 707, 713
BcoDI	GTCTC	4 cut(s)	274, 1131, 1775, 1880
BfaI	CTAG	2 cut(s)	44, 638
BfmI	CTRYAG	2 cut(s)	1141, 1791
BfoI	RGCGCY	2 cut(s)	157, 1042
BfuAI	ACCTGC	1 cut(s)	1909
BfuI	GTATCC	2 cut(s)	1150, 1282
BlpI	GCTNAGC	1 cut(s)	423
BmcAI	AGTACT	2 cut(s)	443, 1331
Bme1390I	CCNGG	4 cut(s)	484, 707, 713, 1019
Bme18I	GGWCC	1 cut(s)	709
BmeT110I	CYCGRG	4 cut(s)	338, 1241, 1778, 1825
BmgT120I	GGNCC	2 cut(s)	274, 709
BmiI	GGNNCC	1 cut(s)	711
BmrFI	CCNGG	4 cut(s)	484, 707, 713, 1019
BmsI	GCATC	7 cut(s)	723, 949, 1099, 1194, 1309, 1339, 1498
BplI	GAGNNNNNCTC	2 cut(s)	1808, 1840
BpmI	CTGGAG	1 cut(s)	1715
Bpu10I	CCTNAGC	4 cut(s)	1218, 1358, 1680, 1686
Bpu1102I	GCTNAGC	1 cut(s)	423
BpuEI	CTTGAG	3 cut(s)	659, 1015, 1445
BpuMI	CCSGG	3 cut(s)	484, 707, 713
BsaBI	GATNNNNATC	1 cut(s)	626
BsaHI	GRCGYC	2 cut(s)	142, 588
BsaJI	CCNNGG	7 cut(s)	129, 483, 558, 705, 882, 918, 1034
BsaWI	WCCGGW	2 cut(s)	1153, 1258
BsaXI	ACNNNNNCTCC	2 cut(s)	798, 828
Bsc4I	CCNNNNNNNGG	5 cut(s)	618, 712, 1247, 1285, 1359
Bse1I	ACTGG	2 cut(s)	433, 1849
Bse8I	GATNNNNATC	1 cut(s)	626
BseAI	TCCGGA	2 cut(s)	1153, 1258
BseBI	CCWGG	1 cut(s)	1019
BseDI	CCNNGG	7 cut(s)	129, 483, 558, 705, 882, 918, 1034
BseGI	GGATG	9 cut(s)	321, 769, 964, 1017, 1114, 1209, 1267, 1561, 1751
BseJI	GATNNNNATC	1 cut(s)	626
BseLI	CCNNNNNNNGG	5 cut(s)	618, 712, 1247, 1285, 1359
BseMII	CTCAG	7 cut(s)	437, 491, 1199, 1209, 1349, 1601, 1905
BseNI	ACTGG	2 cut(s)	433, 1849
BsePI	GCGCGC	1 cut(s)	1089
BseRI	GAGGAG	9 cut(s)	172, 356, 443, 800, 911, 1235, 1316, 1595, 1667
BseX3I	CGGCCG	1 cut(s)	132
BseXI	GCAGC	8 cut(s)	359, 586, 1334, 1712, 1717, 1721, 1807, 1822
BseYI	CCCAGC	1 cut(s)	1511
BsgI	GTGCAG	1 cut(s)	1821
Bsh1236I	CGCG	3 cut(s)	131, 935, 1091
Bsh1285I	CGRYCG	1 cut(s)	135
BshFI	GGCC	8 cut(s)	134, 275, 482, 881, 992, 1312, 1363, 1799
BsiEI	CGRYCG	1 cut(s)	135
BsiHKAI	GWGCWC	2 cut(s)	417, 675
BsiHKCI	CYCGRG	4 cut(s)	338, 1241, 1778, 1825
BsiSI	CCGG	6 cut(s)	282, 483, 707, 713, 1154, 1259
BslFI	GGGAC	1 cut(s)	205
BslI	CCNNNNNNNGG	5 cut(s)	618, 712, 1247, 1285, 1359
BsmAI	GTCTC	4 cut(s)	274, 1131, 1775, 1880
BsmBI	CGTCTC	1 cut(s)	274
BsmFI	GGGAC	1 cut(s)	205
BsmI	GAATGC	1 cut(s)	309
BsnI	GGCC	8 cut(s)	134, 275, 482, 881, 992, 1312, 1363, 1799
BsoBI	CYCGRG	4 cut(s)	338, 1241, 1778, 1825
Bsp1286I	GDGCHC	2 cut(s)	417, 675
Bsp13I	TCCGGA	2 cut(s)	1153, 1258
Bsp143I	GATC	5 cut(s)	334, 579, 627, 1014, 1183
Bsp1720I	GCTNAGC	1 cut(s)	423
Bsp19I	CCATGG	2 cut(s)	558, 882
BspANI	GGCC	8 cut(s)	134, 275, 482, 881, 992, 1312, 1363, 1799
BspCNI	CTCAG	7 cut(s)	436, 490, 1200, 1210, 1350, 1602, 1906
BspEI	TCCGGA	2 cut(s)	1153, 1258
BspFNI	CGCG	3 cut(s)	131, 935, 1091
BspHI	TCATGA	1 cut(s)	858
BspLI	GGNNCC	1 cut(s)	711
BspMAI	CTGCAG	1 cut(s)	1795
BspMI	ACCTGC	1 cut(s)	1909
BspPI	GGATC	1 cut(s)	1009
BsrBI	CCGCTC	2 cut(s)	178, 422
BsrI	ACTGG	2 cut(s)	433, 1849
BssECI	CCNNGG	7 cut(s)	129, 483, 558, 705, 882, 918, 1034
BssHII	GCGCGC	1 cut(s)	1089
BssMI	GATC	5 cut(s)	334, 579, 627, 1014, 1183
BssNI	GRCGYC	2 cut(s)	142, 588
BssT1I	CCWWGG	3 cut(s)	558, 882, 918
Bst2UI	CCWGG	1 cut(s)	1019
Bst4CI	ACNGT	3 cut(s)	219, 403, 1907
BstACI	GRCGYC	2 cut(s)	142, 588
BstAPI	GCANNNNNTGC	1 cut(s)	1730
BstC8I	GCNNGC	2 cut(s)	146, 1091
BstDEI	CTNAG	9 cut(s)	423, 477, 1208, 1218, 1358, 1610, 1680, 1686, 1914
BstDSI	CCRYGG	3 cut(s)	129, 558, 882
BstF5I	GGATG	9 cut(s)	321, 769, 964, 1017, 1114, 1209, 1267, 1561, 1751
BstFNI	CGCG	3 cut(s)	131, 935, 1091
BstH2I	RGCGCY	2 cut(s)	157, 1042
BstHHI	GCGC	5 cut(s)	156, 935, 1041, 1091, 1093
BstKTI	GATC	5 cut(s)	337, 582, 630, 1017, 1186
BstMAI	GTCTC	4 cut(s)	274, 1131, 1775, 1880
BstMBI	GATC	5 cut(s)	334, 579, 627, 1014, 1183
BstMCI	CGRYCG	1 cut(s)	135
BstMWI	GCNNNNNNNGC	7 cut(s)	488, 975, 1706, 1709, 1730, 1736, 1817
BstNI	CCWGG	1 cut(s)	1019
BstSCI	CCNGG	4 cut(s)	482, 705, 711, 1017
BstSFI	CTRYAG	2 cut(s)	1141, 1791
BstUI	CGCG	3 cut(s)	131, 935, 1091
BstV1I	GCAGC	8 cut(s)	359, 586, 1334, 1712, 1717, 1721, 1807, 1822
BstZI	CGGCCG	1 cut(s)	132
BsuI	GTATCC	2 cut(s)	1150, 1282
BsuRI	GGCC	8 cut(s)	134, 275, 482, 881, 992, 1312, 1363, 1799
BtgI	CCRYGG	3 cut(s)	129, 558, 882
BtsCI	GGATG	9 cut(s)	321, 769, 964, 1017, 1114, 1209, 1267, 1561, 1751
BveI	ACCTGC	1 cut(s)	1909
Cac8I	GCNNGC	2 cut(s)	146, 1091
CaiI	CAGNNNCTG	1 cut(s)	1790
CciI	TCATGA	1 cut(s)	858
CfoI	GCGC	5 cut(s)	156, 935, 1041, 1091, 1093
Cfr13I	GGNCC	2 cut(s)	274, 709
Cfr42I	CCGCGG	1 cut(s)	132
CseI	GACGC	2 cut(s)	150, 596
Csp6I	GTAC	2 cut(s)	442, 1330
CspCI	CAANNNNNGTGG	2 cut(s)	7, 42
CviAII	CATG	4 cut(s)	559, 859, 883, 1589
CviQI	GTAC	2 cut(s)	442, 1330
DdeI	CTNAG	9 cut(s)	423, 477, 1208, 1218, 1358, 1610, 1680, 1686, 1914
DpnI	GATC	5 cut(s)	336, 581, 629, 1016, 1185
DpnII	GATC	5 cut(s)	334, 579, 627, 1014, 1183
EaeI	YGGCCR	1 cut(s)	132
EagI	CGGCCG	1 cut(s)	132
EciI	GGCGGA	3 cut(s)	50, 314, 419
Ecl136II	GAGCTC	1 cut(s)	673
EclXI	CGGCCG	1 cut(s)	132
Eco130I	CCWWGG	3 cut(s)	558, 882, 918
Eco147I	AGGCCT	1 cut(s)	992
Eco24I	GRGCYC	1 cut(s)	675
Eco47I	GGWCC	1 cut(s)	709
Eco52I	CGGCCG	1 cut(s)	132
Eco53kI	GAGCTC	1 cut(s)	673
Eco57I	CTGAAG	3 cut(s)	812, 1318, 1695
Eco88I	CYCGRG	4 cut(s)	338, 1241, 1778, 1825
EcoICRI	GAGCTC	1 cut(s)	673
EcoRI	GAATTC	2 cut(s)	751, 1571
EcoRII	CCWGG	1 cut(s)	1017
EcoT14I	CCWWGG	3 cut(s)	558, 882, 918
EcoT22I	ATGCAT	1 cut(s)	964
EcoT38I	GRGCYC	1 cut(s)	675
ErhI	CCWWGG	3 cut(s)	558, 882, 918
Esp3I	CGTCTC	1 cut(s)	274
FaeI	CATG	4 cut(s)	562, 862, 886, 1592
FaqI	GGGAC	1 cut(s)	205
FatI	CATG	4 cut(s)	558, 858, 882, 1588
FauI	CCCGC	1 cut(s)	183
FauNDI	CATATG	1 cut(s)	1273
FbaI	TGATCA	3 cut(s)	579, 627, 1183
FokI	GGATG	9 cut(s)	308, 776, 971, 1024, 1121, 1216, 1274, 1568, 1738
FriOI	GRGCYC	1 cut(s)	675
FspBI	CTAG	2 cut(s)	44, 638
GlaI	GCGC	5 cut(s)	155, 934, 1040, 1090, 1092
GsaI	CCCAGC	1 cut(s)	1515
GsuI	CTGGAG	1 cut(s)	1715
HaeII	RGCGCY	2 cut(s)	157, 1042
HaeIII	GGCC	8 cut(s)	134, 275, 482, 881, 992, 1312, 1363, 1799
HapII	CCGG	6 cut(s)	282, 483, 707, 713, 1154, 1259
HgaI	GACGC	2 cut(s)	150, 596
HhaI	GCGC	5 cut(s)	156, 935, 1041, 1091, 1093
Hin1I	GRCGYC	2 cut(s)	142, 588
Hin1II	CATG	4 cut(s)	562, 862, 886, 1592
Hin6I	GCGC	5 cut(s)	154, 933, 1039, 1089, 1091
HinP1I	GCGC	5 cut(s)	154, 933, 1039, 1089, 1091
HindIII	AAGCTT	3 cut(s)	776, 1060, 1643
HinfI	GANTC	5 cut(s)	165, 758, 1255, 1787, 1892
HpaII	CCGG	6 cut(s)	282, 483, 707, 713, 1154, 1259
HphI	GGTGA	6 cut(s)	307, 391, 409, 448, 934, 1462
Hpy166II	GTNNAC	3 cut(s)	721, 1078, 1269
Hpy188I	TCNGA	7 cut(s)	164, 448, 621, 757, 763, 1337, 1476
Hpy8I	GTNNAC	3 cut(s)	721, 1078, 1269
Hpy99I	CGWCG	1 cut(s)	144
HpyCH4III	ACNGT	3 cut(s)	219, 403, 1907
HpyCH4V	TGCA	9 cut(s)	736, 962, 1322, 1588, 1724, 1793, 1811, 1838, 1910
HpyF10VI	GCNNNNNNNGC	7 cut(s)	488, 975, 1706, 1709, 1730, 1736, 1817
HpyF3I	CTNAG	9 cut(s)	423, 477, 1208, 1218, 1358, 1610, 1680, 1686, 1914
Hsp92I	GRCGYC	2 cut(s)	142, 588
Hsp92II	CATG	4 cut(s)	562, 862, 886, 1592
HspAI	GCGC	5 cut(s)	154, 933, 1039, 1089, 1091
Kpn2I	TCCGGA	2 cut(s)	1153, 1258
Ksp22I	TGATCA	3 cut(s)	579, 627, 1183
KspI	CCGCGG	1 cut(s)	132
Kzo9I	GATC	5 cut(s)	334, 579, 627, 1014, 1183
LmnI	GCTCC	7 cut(s)	261, 432, 670, 787, 1021, 1222, 1690
Lsp1109I	GCAGC	8 cut(s)	359, 586, 1334, 1712, 1717, 1721, 1807, 1822
LweI	GCATC	7 cut(s)	723, 949, 1099, 1194, 1309, 1339, 1498
MaeI	CTAG	2 cut(s)	44, 638
MaeIII	GTNAC	1 cut(s)	1901
MalI	GATC	5 cut(s)	336, 581, 629, 1016, 1185
MbiI	CCGCTC	2 cut(s)	178, 422
MboI	GATC	5 cut(s)	334, 579, 627, 1014, 1183
MboII	GAAGA	7 cut(s)	134, 511, 830, 937, 1141, 1465, 1507
MhlI	GDGCHC	2 cut(s)	417, 675
MlyI	GAGTC	2 cut(s)	159, 1796
MmeI	TCCRAC	3 cut(s)	187, 402, 471
Mph1103I	ATGCAT	1 cut(s)	964
MroI	TCCGGA	2 cut(s)	1153, 1258
MseI	TTAA	6 cut(s)	71, 1065, 1104, 1404, 1533, 1599
MspA1I	CMGCKG	4 cut(s)	131, 148, 577, 1325
MspI	CCGG	6 cut(s)	282, 483, 707, 713, 1154, 1259
MspR9I	CCNGG	4 cut(s)	484, 707, 713, 1019
Mva1269I	GAATGC	1 cut(s)	309
MvaI	CCWGG	1 cut(s)	1019
MvnI	CGCG	3 cut(s)	131, 935, 1091
MwoI	GCNNNNNNNGC	7 cut(s)	488, 975, 1706, 1709, 1730, 1736, 1817
NciI	CCSGG	3 cut(s)	484, 707, 713
NcoI	CCATGG	2 cut(s)	558, 882
NdeI	CATATG	1 cut(s)	1273
NdeII	GATC	5 cut(s)	334, 579, 627, 1014, 1183
NlaIII	CATG	4 cut(s)	562, 862, 886, 1592
NlaIV	GGNNCC	1 cut(s)	711
NmeAIII	GCCGAG	2 cut(s)	251, 1909
NmuCI	GTSAC	1 cut(s)	1901
NsiI	ATGCAT	1 cut(s)	964
PaeR7I	CTCGAG	2 cut(s)	338, 1825
PagI	TCATGA	1 cut(s)	858
PauI	GCGCGC	1 cut(s)	1089
PceI	AGGCCT	1 cut(s)	992
PcsI	WCGNNNNNNNCGW	1 cut(s)	117
PctI	GAATGC	1 cut(s)	309
PfeI	GAWTC	3 cut(s)	758, 1255, 1892
PflFI	GACNNNGTC	1 cut(s)	1786
PfoI	TCCNGGA	1 cut(s)	1017
PleI	GAGTC	2 cut(s)	159, 1795
PpsI	GAGTC	2 cut(s)	159, 1795
PsiI	TTATAA	3 cut(s)	945, 1380, 1490
Psp124BI	GAGCTC	1 cut(s)	675
Psp6I	CCWGG	1 cut(s)	1017
PspFI	CCCAGC	1 cut(s)	1511
PspGI	CCWGG	1 cut(s)	1017
PspN4I	GGNNCC	1 cut(s)	711
PspPI	GGNCC	2 cut(s)	274, 709
PspXI	VCTCGAGB	1 cut(s)	1825
PstI	CTGCAG	1 cut(s)	1795
PstNI	CAGNNNCTG	1 cut(s)	1790
PsyI	GACNNNGTC	1 cut(s)	1786
PteI	GCGCGC	1 cut(s)	1089
PvuII	CAGCTG	1 cut(s)	577
RsaI	GTAC	2 cut(s)	443, 1331
RsaNI	GTAC	2 cut(s)	442, 1330
SacI	GAGCTC	1 cut(s)	675
SacII	CCGCGG	1 cut(s)	132
SaqAI	TTAA	6 cut(s)	71, 1065, 1104, 1404, 1533, 1599
Sau3AI	GATC	5 cut(s)	334, 579, 627, 1014, 1183
Sau96I	GGNCC	2 cut(s)	274, 709
ScaI	AGTACT	2 cut(s)	443, 1331
SchI	GAGTC	2 cut(s)	159, 1796
ScrFI	CCNGG	4 cut(s)	484, 707, 713, 1019
SduI	GDGCHC	2 cut(s)	417, 675
SfaNI	GCATC	7 cut(s)	723, 949, 1099, 1194, 1309, 1339, 1498
SfcI	CTRYAG	2 cut(s)	1141, 1791
Sfr274I	CTCGAG	2 cut(s)	338, 1825
Sfr303I	CCGCGG	1 cut(s)	132
SgrBI	CCGCGG	1 cut(s)	132
SinI	GGWCC	1 cut(s)	709
SlaI	CTCGAG	2 cut(s)	338, 1825
SmlI	CTYRAG	5 cut(s)	338, 674, 994, 1460, 1825
SmoI	CTYRAG	5 cut(s)	338, 674, 994, 1460, 1825
SseBI	AGGCCT	1 cut(s)	992
SspMI	CTAG	2 cut(s)	44, 638
SstI	GAGCTC	1 cut(s)	675
StuI	AGGCCT	1 cut(s)	992
StyD4I	CCNGG	4 cut(s)	482, 705, 711, 1017
StyI	CCWWGG	3 cut(s)	558, 882, 918
TaaI	ACNGT	3 cut(s)	219, 403, 1907
TaqI	TCGA	7 cut(s)	168, 339, 783, 1440, 1521, 1575, 1826
TatI	WGTACW	2 cut(s)	441, 1329
TauI	GCSGC	4 cut(s)	66, 131, 134, 159
TfiI	GAWTC	3 cut(s)	758, 1255, 1892
Tru1I	TTAA	6 cut(s)	71, 1065, 1104, 1404, 1533, 1599
Tru9I	TTAA	6 cut(s)	71, 1065, 1104, 1404, 1533, 1599
TseFI	GTSAC	1 cut(s)	1901
TseI	GCWGC	8 cut(s)	347, 574, 1322, 1700, 1709, 1730, 1820, 1835
Tsp45I	GTSAC	1 cut(s)	1901
TspDTI	ATGAA	4 cut(s)	460, 847, 860, 1508
TspGWI	ACGGA	2 cut(s)	73, 1410
Tth111I	GACNNNGTC	1 cut(s)	1786
VpaK11BI	GGWCC	1 cut(s)	709
XapI	RAATTY	4 cut(s)	751, 1051, 1571, 1876
XhoI	CTCGAG	2 cut(s)	338, 1825
XspI	CTAG	2 cut(s)	44, 638
ZrmI	AGTACT	2 cut(s)	443, 1331
Zsp2I	ATGCAT	1 cut(s)	964

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.