Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0000943

Physical Location & Seq

Genomic Coordinates Reverse (-)

1314102 .. 1317713

Gene Structure 3612 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1017100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1239 bp

ATGAGCAGCCAGCAGAGACGTCCGCCGGACCCAGTGGCGGTGCTGAGAGGCCACCGCGCCTCCGTCATGGACCTTTCTTTTCATCCTTACCTACCTCTCCTGTTCACCGGTTCATCAGACGGCGAGTTGCGGATTTGGGATACTATCCAGCATCGGACTGTATCCTCAGCAGGGGTGCATAATGCAGCACATGGTATTGCTTCTGTTGCTTGTACTGCCAAAAGAGTTATCAGCCAGGGTAGGGATGGAACGGTTAAGTGTTGGGATATTGAAGATGGAGGTCTGTCCAGGGTTCCATCTGTTACAATCAAGACAAATTCTTACCACTTTTGTAAACTTTCCTTGGTGAAGAGGCCTCAATCTTGTTCTACACGGATGGACGGGGTTAGGTTTTGTGACGGAGAGGAGAGAAGGGTGAAGGGGACTACCGATGAAGATACTTTGGACGACAGTAGAGACAACGGTGAAGGGGATCTGCCTGAGCAGCCCAGCGCCTTTGAAGAAAATACTCAAGATGAAGGATCTAAATATGTCGCTGTAGCAGGGGAGCAGACTTCTGAGGTTGAGATTTGGGATCTTAGTACTGCAGAAAGGTTTGCACGGTTGCCTCAAAGCTGCTCTGGTGGCTCCTTGGGTATTCCTTCCAAGGAGAGCGGTCCCTTTCTAATTCATTCAACCTTTGGCTTACTAGTTTCTGGATTGGATTGTGTTTCAGTTTATAGCCGACGAATGTGCATGGCAATTCAAGCATTCTCACCGTCTGAATCACCGGGATTCTTAAATGTCTTGGTCGGTTATGAGGATGGTTCCATGCTTTGGTGGGATATTAGAAATCCGGGGGTTCCAATGACCACTGTCAAGTTTCATTCAGAGCCAGTTCTAAGTCTATGCGTTGATGGGTCATGCAATGGTGGTATCTCAGGAGCCGCTGATGAGAAAGTTGTGCTGTATAGTTTGGATCCTTCAATGGGTACGTGCGTGATCAAGAAAGAAATTGTTTTGGAACGACCTGGAATTTCAAGCACTTCCATTCGACCTGACGGTAAAATTTTTGCAACAGCTGGTTGGGACCACAGAGTGAGGGTGTATAACTATCGTAAAGGAAATGCTTTGGCGATATTGAGGTATCACCATGCAACGTGTAATGCGGTTTCTTATTCCGGTGATTGTAAGCTAATGGCATCTGCATCAGAAGATACGACGGTGGCGCTATGGGAACTCTACCCTCCTCAAACTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000075 GO:0000151 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006282 GO:0006810 GO:0006950 GO:0006974 GO:0007275 GO:0008150 GO:0009555 GO:0009636 GO:0009663 GO:0009790 GO:0009791 GO:0009793 GO:0009908 GO:0009987 GO:0010035 GO:0010038 GO:0010044 GO:0010154 GO:0010162 GO:0010431 GO:0010496 GO:0010497 GO:0010564 GO:0016043 GO:0019219 GO:0019222 GO:0021700 GO:0022414 GO:0022611 GO:0031323 GO:0031461 GO:0031570 GO:0032501 GO:0032502 GO:0032504 GO:0032991 GO:0033554 GO:0034330 GO:0035670 GO:0042221 GO:0042493 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045216 GO:0045786 GO:0045787 GO:0048229 GO:0048316 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048481 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048608 GO:0048609 GO:0048700 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051052 GO:0051171 GO:0051179 GO:0051234 GO:0051239 GO:0051716 GO:0051726 GO:0060255 GO:0061458 GO:0065007 GO:0071156 GO:0071158 GO:0071695 GO:0071840 GO:0072718 GO:0080008 GO:0080090 GO:0080134 GO:0080135 GO:0090068 GO:0090567 GO:0097327 GO:0097439 GO:0099402 GO:1902494 GO:1990234 GO:2000026 GO:2000280 GO:2001020

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

413

Amino Acids

44.9

Weight (kDa)

5.7

Isoelectric Point (pI)

48.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40	PF00400	11 - 47	9.7e-09	WD domain, G-beta repeat
Beta-prop_WDR5	PF25175	12 - 95	5.6e-11	WDR5 beta-propeller domain
WD40_Prp19	PF24814	12 - 94	1.2e-07	Prp19 WD40 domain
Beta-prop_WDR3_1st	PF25173	13 - 92	4.7e-11	WDR3 first beta-propeller domain
Beta-prop_THOC3	PF25174	14 - 111	1.8e-09	THOC3 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	15 - 89	1.7e-06	WDR3 second beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	33 - 100	2.8e-09	TEP-1 second beta-propeller
Beta-prop_WDR3_2nd	PF25172	257 - 406	3.1e-10	WDR3 second beta-propeller domain
WD40_Prp19	PF24814	261 - 407	5e-16	Prp19 WD40 domain
WD40_CDC20-Fz	PF24807	262 - 407	1.2e-09	CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	262 - 407	6e-07	WDR36/Utp21 second beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	263 - 407	4e-06	TEP-1 second beta-propeller
Beta-prop_THOC3	PF25174	266 - 407	5.9e-14	THOC3 beta-propeller domain
WD40_Gbeta	PF25391	266 - 406	1.2e-06	G protein beta WD-40 repeat protein
Beta-prop_EML_2	PF23414	267 - 407	2.2e-06	Echinoderm microtubule-associated protein second beta-propeller
WD40_MABP1-WDR62_2nd	PF24782	296 - 407	1.4e-06	MABP1/WDR62 second WD40 domain
Beta-prop_WDR5	PF25175	337 - 407	1.1e-13	WDR5 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	339 - 407	3e-08	WDR3 first beta-propeller domain
WD40_WDHD1_1st	PF24817	341 - 407	3.3e-06	WDHD1 first WD40 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000943)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G29860
fragaria_vesca	FvH4_2g39120 FvH4_2g39120 FvH4_2g39120 FvH4_2g39120 FvH4_2g39120 FvH4_2g39120 FvH4_2g39120
malus_domestica	MD08G1017100.v1.1 MD15G1016100.v1.1 MD15G1017900.v1.1 MD15G1018000.v1.1
prunus_persica	Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1
pyrus_communis	pycom08g01490 pycom15g01390
rosa_chinensis	RchiOBHm_Chr6g0272131 RchiOBHm_Chr6g0302231
rosa_laevigata	RLG00000011183 RLG00000013699
rosa_multiflora	Rmu_sc0013301.1_g000007 Rmu_sc0027601.1_g000001
rosa_roxburghii	Rroxscaffold_7G00165940 Rroxscaffold_7G00196580
rosa_rugosa	Rorug06G0065900 Rorug06G0313400.1
rosa_samantha	Rh6AG183100 Rh6BG184000 Rh6BG476300 Rh6CG184400 Rh6DG176100 Rh6DG425800
rosa_wichuraiana	Rw4G012510 Rw6G015760 Rw6G036890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	22
AccB7I	CCANNNNNTGG	1 cut(s)	816
AccBSI	CCGCTC	1 cut(s)	654
AccII	CGCG	1 cut(s)	57
AciI	CCGC	7 cut(s)	23, 38, 55, 130, 654, 927, 1148
AclWI	GGATC	5 cut(s)	480, 529, 582, 953, 966
AcsI	RAATTY	3 cut(s)	316, 1014, 1047
AcyI	GRCGYC	1 cut(s)	19
AdeI	CACNNNGTG	1 cut(s)	1078
AfaI	GTAC	3 cut(s)	214, 583, 973
AfiI	CCNNNNNNNGG	5 cut(s)	37, 171, 241, 816, 968
AflIII	ACRYGT	1 cut(s)	1139
AgeI	ACCGGT	1 cut(s)	107
AgsI	TTSAA	6 cut(s)	272, 500, 675, 746, 966, 1020
AhlI	ACTAGT	1 cut(s)	688
AjnI	CCWGG	3 cut(s)	234, 287, 1009
AluBI	AGCT	3 cut(s)	615, 1061, 1174
AluI	AGCT	3 cut(s)	615, 1061, 1174
Alw26I	GTCTC	2 cut(s)	10, 450
AlwI	GGATC	5 cut(s)	480, 529, 582, 953, 966
AoxI	GGCC	2 cut(s)	49, 353
ApeKI	GCWGC	4 cut(s)	6, 185, 484, 615
ApoI	RAATTY	3 cut(s)	316, 1014, 1047
AsiGI	ACCGGT	1 cut(s)	107
AspLEI	GCGC	3 cut(s)	59, 494, 1210
AspS9I	GGNCC	4 cut(s)	28, 70, 656, 1069
AsuC2I	CCSGG	2 cut(s)	771, 837
AsuHPI	GGTGA	8 cut(s)	97, 358, 427, 476, 747, 759, 1121, 1175
AvaII	GGWCC	4 cut(s)	28, 70, 656, 1069
BaeI	ACNNNNGTAYC	4 cut(s)	963, 996, 1188, 1221
BamHI	GGATCC	1 cut(s)	958
BbvCI	CCTCAGC	1 cut(s)	166
BbvI	GCAGC	4 cut(s)	18, 197, 496, 602
BccI	CCATC	6 cut(s)	239, 269, 304, 370, 797, 890
BceAI	ACGGC	1 cut(s)	136
BcgI	CGANNNNNNTGC	2 cut(s)	714, 748
BciT130I	CCWGG	3 cut(s)	236, 289, 1011
BciVI	GTATCC	2 cut(s)	133, 172
BclI	TGATCA	1 cut(s)	981
BcnI	CCSGG	2 cut(s)	771, 837
BcoDI	GTCTC	2 cut(s)	10, 450
BcuI	ACTAGT	1 cut(s)	688
BfaI	CTAG	1 cut(s)	689
BfmI	CTRYAG	2 cut(s)	537, 585
BfoI	RGCGCY	2 cut(s)	495, 1211
BfuI	GTATCC	2 cut(s)	133, 172
BisI	GCNGC	5 cut(s)	7, 186, 485, 616, 927
BlsI	GCNGC	5 cut(s)	8, 187, 486, 617, 928
BmcAI	AGTACT	1 cut(s)	583
Bme1390I	CCNGG	5 cut(s)	236, 289, 771, 837, 1011
Bme18I	GGWCC	4 cut(s)	28, 70, 656, 1069
BmgT120I	GGNCC	4 cut(s)	28, 70, 656, 1069
BmiI	GGNNCC	9 cut(s)	30, 294, 628, 658, 808, 843, 925, 960, 1070
BmrFI	CCNGG	5 cut(s)	236, 289, 771, 837, 1011
BmrI	ACTGGG	1 cut(s)	26
BmsI	GCATC	3 cut(s)	160, 1190, 1196
BmuI	ACTGGG	1 cut(s)	26
BoxI	GACNNNNGTC	1 cut(s)	854
Bpu10I	CCTNAGC	2 cut(s)	166, 480
BpuEI	CTTGAG	1 cut(s)	495
BpuMI	CCSGG	2 cut(s)	771, 837
BsaAI	YACGTR	1 cut(s)	975
BsaHI	GRCGYC	1 cut(s)	19
BsaJI	CCNNGG	6 cut(s)	235, 288, 342, 630, 645, 836
BsaWI	WCCGGW	2 cut(s)	107, 1160
BsaXI	ACNNNNNCTCC	2 cut(s)	44, 74
Bsc4I	CCNNNNNNNGG	5 cut(s)	37, 171, 241, 816, 968
Bse118I	RCCGGY	1 cut(s)	107
Bse1I	ACTGG	2 cut(s)	32, 875
Bse3DI	GCAATG	1 cut(s)	913
BseBI	CCWGG	3 cut(s)	236, 289, 1011
BseDI	CCNNGG	6 cut(s)	235, 288, 342, 630, 645, 836
BseGI	GGATG	4 cut(s)	82, 250, 381, 808
BseLI	CCNNNNNNNGG	5 cut(s)	37, 171, 241, 816, 968
BseMI	GCAATG	1 cut(s)	913
BseMII	CTCAG	5 cut(s)	35, 180, 471, 549, 933
BseNI	ACTGG	2 cut(s)	32, 875
BseRI	GAGGAG	2 cut(s)	419, 1218
BseXI	GCAGC	4 cut(s)	18, 197, 496, 602
BseYI	CCCAGC	1 cut(s)	488
Bsh1236I	CGCG	1 cut(s)	57
BshFI	GGCC	2 cut(s)	51, 355
BshTI	ACCGGT	1 cut(s)	107
BsiSI	CCGG	5 cut(s)	26, 108, 770, 836, 1161
BslFI	GGGAC	3 cut(s)	436, 642, 1082
BslI	CCNNNNNNNGG	5 cut(s)	37, 171, 241, 816, 968
BsmAI	GTCTC	2 cut(s)	10, 450
BsmBI	CGTCTC	1 cut(s)	10
BsmFI	GGGAC	3 cut(s)	436, 642, 1082
BsmI	GAATGC	1 cut(s)	749
BsnI	GGCC	2 cut(s)	51, 355
Bsp143I	GATC	5 cut(s)	472, 521, 574, 958, 981
BspACI	CCGC	7 cut(s)	23, 38, 55, 130, 654, 927, 1148
BspANI	GGCC	2 cut(s)	51, 355
BspCNI	CTCAG	5 cut(s)	36, 179, 472, 550, 932
BspFNI	CGCG	1 cut(s)	57
BspLI	GGNNCC	9 cut(s)	30, 294, 628, 658, 808, 843, 925, 960, 1070
BspMAI	CTGCAG	1 cut(s)	589
BspPI	GGATC	5 cut(s)	480, 529, 582, 953, 966
BsrBI	CCGCTC	1 cut(s)	654
BsrDI	GCAATG	1 cut(s)	913
BsrFI	RCCGGY	1 cut(s)	107
BsrI	ACTGG	2 cut(s)	32, 875
BssAI	RCCGGY	1 cut(s)	107
BssECI	CCNNGG	6 cut(s)	235, 288, 342, 630, 645, 836
BssMI	GATC	5 cut(s)	472, 521, 574, 958, 981
BssNI	GRCGYC	1 cut(s)	19
BssT1I	CCWWGG	3 cut(s)	342, 630, 645
Bst2UI	CCWGG	3 cut(s)	236, 289, 1011
Bst4CI	ACNGT	9 cut(s)	160, 253, 452, 464, 603, 759, 856, 1043, 1204
Bst6I	CTCTTC	1 cut(s)	344
BstACI	GRCGYC	1 cut(s)	19
BstBAI	YACGTR	1 cut(s)	975
BstC8I	GCNNGC	1 cut(s)	11
BstDEI	CTNAG	7 cut(s)	44, 166, 480, 558, 578, 881, 919
BstF5I	GGATG	4 cut(s)	82, 250, 381, 808
BstFNI	CGCG	1 cut(s)	57
BstH2I	RGCGCY	2 cut(s)	495, 1211
BstHHI	GCGC	3 cut(s)	59, 494, 1210
BstKTI	GATC	5 cut(s)	475, 524, 577, 961, 984
BstMAI	GTCTC	2 cut(s)	10, 450
BstMBI	GATC	5 cut(s)	472, 521, 574, 958, 981
BstMWI	GCNNNNNNNGC	5 cut(s)	206, 215, 484, 624, 746
BstNI	CCWGG	3 cut(s)	236, 289, 1011
BstPAI	GACNNNNGTC	1 cut(s)	854
BstSCI	CCNGG	5 cut(s)	234, 287, 769, 835, 1009
BstSFI	CTRYAG	2 cut(s)	537, 585
BstUI	CGCG	1 cut(s)	57
BstV1I	GCAGC	4 cut(s)	18, 197, 496, 602
BstX2I	RGATCY	4 cut(s)	472, 521, 574, 958
BstYI	RGATCY	4 cut(s)	472, 521, 574, 958
BsuI	GTATCC	2 cut(s)	133, 172
BsuRI	GGCC	2 cut(s)	51, 355
BtsCI	GGATG	4 cut(s)	82, 250, 381, 808
BtsIMutI	CAGTG	2 cut(s)	39, 852
Cac8I	GCNNGC	1 cut(s)	11
CfoI	GCGC	3 cut(s)	59, 494, 1210
Cfr10I	RCCGGY	1 cut(s)	107
Cfr13I	GGNCC	4 cut(s)	28, 70, 656, 1069
Csp6I	GTAC	3 cut(s)	213, 582, 972
CspAI	ACCGGT	1 cut(s)	107
CviAII	CATG	6 cut(s)	67, 191, 736, 811, 903, 1133
CviQI	GTAC	3 cut(s)	213, 582, 972
DdeI	CTNAG	7 cut(s)	44, 166, 480, 558, 578, 881, 919
DpnI	GATC	5 cut(s)	474, 523, 576, 960, 983
DpnII	GATC	5 cut(s)	472, 521, 574, 958, 981
DraIII	CACNNNGTG	1 cut(s)	1078
Eam1104I	CTCTTC	1 cut(s)	344
EarI	CTCTTC	1 cut(s)	344
EciI	GGCGGA	1 cut(s)	12
Eco130I	CCWWGG	3 cut(s)	342, 630, 645
Eco147I	AGGCCT	1 cut(s)	355
Eco47I	GGWCC	4 cut(s)	28, 70, 656, 1069
EcoRII	CCWGG	3 cut(s)	234, 287, 1009
EcoT14I	CCWWGG	3 cut(s)	342, 630, 645
ErhI	CCWWGG	3 cut(s)	342, 630, 645
Esp3I	CGTCTC	1 cut(s)	10
FaeI	CATG	6 cut(s)	70, 194, 739, 814, 906, 1136
FaqI	GGGAC	3 cut(s)	436, 642, 1082
FatI	CATG	6 cut(s)	66, 190, 735, 810, 902, 1132
FbaI	TGATCA	1 cut(s)	981
Fnu4HI	GCNGC	5 cut(s)	7, 186, 485, 616, 927
FokI	GGATG	4 cut(s)	69, 257, 388, 815
Fsp4HI	GCNGC	5 cut(s)	7, 186, 485, 616, 927
FspBI	CTAG	1 cut(s)	689
GlaI	GCGC	3 cut(s)	58, 493, 1209
GluI	GCNGC	5 cut(s)	7, 186, 485, 616, 927
GsaI	CCCAGC	1 cut(s)	492
HaeII	RGCGCY	2 cut(s)	495, 1211
HaeIII	GGCC	2 cut(s)	51, 355
HapII	CCGG	5 cut(s)	26, 108, 770, 836, 1161
HhaI	GCGC	3 cut(s)	59, 494, 1210
Hin1I	GRCGYC	1 cut(s)	19
Hin1II	CATG	6 cut(s)	70, 194, 739, 814, 906, 1136
Hin6I	GCGC	3 cut(s)	57, 492, 1208
HinP1I	GCGC	3 cut(s)	57, 492, 1208
HinfI	GANTC	2 cut(s)	764, 774
HpaII	CCGG	5 cut(s)	26, 108, 770, 836, 1161
HphI	GGTGA	8 cut(s)	97, 358, 427, 476, 747, 759, 1121, 1175
Hpy166II	GTNNAC	2 cut(s)	105, 335
Hpy188I	TCNGA	6 cut(s)	118, 156, 559, 763, 871, 1192
Hpy188III	TCNNGA	5 cut(s)	310, 512, 696, 921, 985
Hpy8I	GTNNAC	2 cut(s)	105, 335
Hpy99I	CGWCG	2 cut(s)	729, 1204
HpyAV	CCTTC	6 cut(s)	405, 412, 461, 512, 651, 972
HpyCH4III	ACNGT	9 cut(s)	160, 253, 452, 464, 603, 759, 856, 1043, 1204
HpyCH4IV	ACGT	3 cut(s)	19, 974, 1139
HpyCH4V	TGCA	9 cut(s)	178, 185, 587, 599, 735, 906, 1055, 1136, 1187
HpyF10VI	GCNNNNNNNGC	5 cut(s)	206, 215, 484, 624, 746
HpyF3I	CTNAG	7 cut(s)	44, 166, 480, 558, 578, 881, 919
HpySE526I	ACGT	3 cut(s)	19, 974, 1139
Hsp92I	GRCGYC	1 cut(s)	19
Hsp92II	CATG	6 cut(s)	70, 194, 739, 814, 906, 1136
HspAI	GCGC	3 cut(s)	57, 492, 1208
Ksp22I	TGATCA	1 cut(s)	981
Kzo9I	GATC	5 cut(s)	472, 521, 574, 958, 981
LmnI	GCTCC	3 cut(s)	547, 632, 923
Lsp1109I	GCAGC	4 cut(s)	18, 197, 496, 602
LweI	GCATC	3 cut(s)	160, 1190, 1196
MaeI	CTAG	1 cut(s)	689
MaeII	ACGT	3 cut(s)	19, 974, 1139
MaeIII	GTNAC	2 cut(s)	301, 395
MalI	GATC	5 cut(s)	474, 523, 576, 960, 983
MbiI	CCGCTC	1 cut(s)	654
MboI	GATC	5 cut(s)	472, 521, 574, 958, 981
MboII	GAAGA	5 cut(s)	284, 361, 446, 512, 1205
MflI	RGATCY	4 cut(s)	472, 521, 574, 958
MluCI	AATT	6 cut(s)	316, 666, 741, 993, 1014, 1047
MseI	TTAA	3 cut(s)	255, 779, 1237
MspA1I	CMGCKG	2 cut(s)	929, 1061
MspI	CCGG	5 cut(s)	26, 108, 770, 836, 1161
MspR9I	CCNGG	5 cut(s)	236, 289, 771, 837, 1011
Mva1269I	GAATGC	1 cut(s)	749
MvaI	CCWGG	3 cut(s)	236, 289, 1011
MvnI	CGCG	1 cut(s)	57
MwoI	GCNNNNNNNGC	5 cut(s)	206, 215, 484, 624, 746
NciI	CCSGG	2 cut(s)	771, 837
NdeII	GATC	5 cut(s)	472, 521, 574, 958, 981
NlaIII	CATG	6 cut(s)	70, 194, 739, 814, 906, 1136
NlaIV	GGNNCC	9 cut(s)	30, 294, 628, 658, 808, 843, 925, 960, 1070
NmuCI	GTSAC	1 cut(s)	395
PceI	AGGCCT	1 cut(s)	355
PctI	GAATGC	1 cut(s)	749
PfeI	GAWTC	2 cut(s)	764, 774
PflMI	CCANNNNNTGG	1 cut(s)	816
PinAI	ACCGGT	1 cut(s)	107
PkrI	GCNGC	5 cut(s)	8, 187, 486, 617, 928
Ppu21I	YACGTR	1 cut(s)	975
PshAI	GACNNNNGTC	1 cut(s)	854
Psp6I	CCWGG	3 cut(s)	234, 287, 1009
PspFI	CCCAGC	1 cut(s)	488
PspGI	CCWGG	3 cut(s)	234, 287, 1009
PspN4I	GGNNCC	9 cut(s)	30, 294, 628, 658, 808, 843, 925, 960, 1070
PspPI	GGNCC	4 cut(s)	28, 70, 656, 1069
PstI	CTGCAG	1 cut(s)	589
PsuI	RGATCY	4 cut(s)	472, 521, 574, 958
PvuII	CAGCTG	1 cut(s)	1061
RsaI	GTAC	3 cut(s)	214, 583, 973
RsaNI	GTAC	3 cut(s)	213, 582, 972
SaqAI	TTAA	3 cut(s)	255, 779, 1237
SatI	GCNGC	5 cut(s)	7, 186, 485, 616, 927
Sau3AI	GATC	5 cut(s)	472, 521, 574, 958, 981
Sau96I	GGNCC	4 cut(s)	28, 70, 656, 1069
ScaI	AGTACT	1 cut(s)	583
ScrFI	CCNGG	5 cut(s)	236, 289, 771, 837, 1011
SfaNI	GCATC	3 cut(s)	160, 1190, 1196
SfcI	CTRYAG	2 cut(s)	537, 585
SinI	GGWCC	4 cut(s)	28, 70, 656, 1069
SmlI	CTYRAG	1 cut(s)	510
SmoI	CTYRAG	1 cut(s)	510
SpeI	ACTAGT	1 cut(s)	688
Sse9I	AATT	6 cut(s)	316, 666, 741, 993, 1014, 1047
SseBI	AGGCCT	1 cut(s)	355
SsiI	CCGC	7 cut(s)	23, 38, 55, 130, 654, 927, 1148
SspMI	CTAG	1 cut(s)	689
StuI	AGGCCT	1 cut(s)	355
StyD4I	CCNGG	5 cut(s)	234, 287, 769, 835, 1009
StyI	CCWWGG	3 cut(s)	342, 630, 645
TaaI	ACNGT	9 cut(s)	160, 253, 452, 464, 603, 759, 856, 1043, 1204
TaiI	ACGT	3 cut(s)	22, 977, 1142
TaqI	TCGA	1 cut(s)	1033
TasI	AATT	6 cut(s)	316, 666, 741, 993, 1014, 1047
TatI	WGTACW	2 cut(s)	212, 581
TauI	GCSGC	1 cut(s)	929
TfiI	GAWTC	2 cut(s)	764, 774
Tru1I	TTAA	3 cut(s)	255, 779, 1237
Tru9I	TTAA	3 cut(s)	255, 779, 1237
TscAI	CASTG	2 cut(s)	39, 859
TseFI	GTSAC	1 cut(s)	395
TseI	GCWGC	4 cut(s)	6, 185, 484, 615
Tsp45I	GTSAC	1 cut(s)	395
TspDTI	ATGAA	6 cut(s)	71, 102, 447, 531, 659, 854
TspGWI	ACGGA	3 cut(s)	52, 388, 414
TspRI	CASTG	2 cut(s)	39, 859
Van91I	CCANNNNNTGG	1 cut(s)	816
VpaK11BI	GGWCC	4 cut(s)	28, 70, 656, 1069
XapI	RAATTY	3 cut(s)	316, 1014, 1047
XcmI	CCANNNNNNNNNTGG	1 cut(s)	242
XspI	CTAG	1 cut(s)	689
ZraI	GACGTC	1 cut(s)	20
ZrmI	AGTACT	1 cut(s)	583

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.