Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0000943

Physical Location & Seq

Genomic Coordinates Reverse (-)

946582 .. 950534

Gene Structure 3953 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1016100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1167 bp

ATGAGCAGCAAGCAGAGACGGCCGCCGGACCCAGTGGCGGTTCTGAGGGGCCACCGCGCCTCCGTCATGGACCTCTCTTTTCATCCTTCCCAACCACTCCTGTTCACCGGTTCATCAGATGGCGAACTGAGGATTTGGGATACTATCCAGCATCGGACTGTATCATCAGCTGGGGTGCATAATGCAGCACATGGTATTGCTTCTGTTTCTTGTACTGCCAACAGAGTTATCAGCCAGGGCAGGGATGGGACGGTTAAATGTTGGGATATTGAAGGTGGAGGTCTGCCCAGGACTCCGTCTGTTACAATCAAGACAAACTCTTACCACTTTTGTAAACTTTCCTTGGTGAAGAGGCCTCAATCTTGTTCTACACGAGTGGACGGGGTTAAGTGTCAGGATGGAGAGGACAGAAGGGTTAGGGGGACCACTGATGAAGATACTCTAGATGACAGTAGAGACAACGTTGAAGGGAATCTGCCTGAGCATCCCAGCGCCTTTGAAGAAAATACTCAAGATGAATTATCTAAATATGTCACTGTAGCAGGGGAGCAGACTTCTGAGGTTGAGATTTGGGATCTTAATACTACAGAAAGGTTTGCACGGCTTCCTCAAAGCTGCTTTGGTGGTTCCCCGGGTATTTCTTCCAAGGAGAGAGGAATGTGCATGGCTATTCAAGCATTCTCACCATCTGAATCACAGGGATTCCTAAATGTCTTGGTTGGTTATGAGGATGGTTCCATGCTTTGGTGGGATATTAGAAATCCGGGGGTTCCAATGACCTCTGTCAGGTTTCATTCAGAACCAGTTCTAAGTCTATGCGTGGATGGGTCATGCAATGGTGGTATCTCAGGAGCTGCAGATGAGAAAGTTGTGCTGTATAGTTTGGATCCTTCAATGGGTACGTGTGTGATCAAGAAAGAAATTATTTTGGAACGACCTGGAATTTCAAGCACTTCCATTCGACCTGATGGTAAAATTTTTGCAACCGCTGGCTGGGACCACAGAGTGAGGGTGTATAACTATCGTAAAGGAAATGCTTTGGCGATATTGAAGTATCACCATGCAACGTGTAATGCCGTTTCTTATTCAGCTGATTGTAAGCTAATGGCATCTGCTTCAGAAGACACAACAGTGGCTCTATGGGAACTCTATCCTCCTCGAACTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000075 GO:0000151 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006282 GO:0006810 GO:0006950 GO:0006974 GO:0007275 GO:0008150 GO:0009555 GO:0009636 GO:0009663 GO:0009790 GO:0009791 GO:0009793 GO:0009908 GO:0009987 GO:0010035 GO:0010038 GO:0010044 GO:0010154 GO:0010162 GO:0010431 GO:0010496 GO:0010497 GO:0010564 GO:0016043 GO:0019219 GO:0019222 GO:0021700 GO:0022414 GO:0022611 GO:0031323 GO:0031461 GO:0031570 GO:0032501 GO:0032502 GO:0032504 GO:0032991 GO:0033554 GO:0034330 GO:0035670 GO:0042221 GO:0042493 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045216 GO:0045786 GO:0045787 GO:0048229 GO:0048316 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048481 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048608 GO:0048609 GO:0048700 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051052 GO:0051171 GO:0051179 GO:0051234 GO:0051239 GO:0051716 GO:0051726 GO:0060255 GO:0061458 GO:0065007 GO:0071156 GO:0071158 GO:0071695 GO:0071840 GO:0072718 GO:0080008 GO:0080090 GO:0080134 GO:0080135 GO:0090068 GO:0090567 GO:0097327 GO:0097439 GO:0099402 GO:1902494 GO:1990234 GO:2000026 GO:2000280 GO:2001020

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

389

Amino Acids

42.45

Weight (kDa)

6.02

Isoelectric Point (pI)

43.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40	PF00400	11 - 47	7.7e-10	WD domain, G-beta repeat
Beta-prop_WDR5	PF25175	12 - 95	9.8e-12	WDR5 beta-propeller domain
WD40_Prp19	PF24814	12 - 93	1.1e-08	Prp19 WD40 domain
Beta-prop_WDR3_1st	PF25173	13 - 91	4.2e-12	WDR3 first beta-propeller domain
EIF3I	PF24805	14 - 200	8.3e-06	EIF3I
Beta-prop_THOC3	PF25174	14 - 195	5.2e-12	THOC3 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	15 - 89	3.1e-07	WDR3 second beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	17 - 97	2.4e-10	TEP-1 second beta-propeller
WD40_CDC20-Fz	PF24807	19 - 93	1.3e-06	CDC20/Fizzy WD40 domain
Beta-prop_IFT140_1st	PF23383	20 - 102	7.9e-07	IFT140 first beta-propeller
Beta-prop_WDR3_1st	PF25173	184 - 301	7.8e-06	WDR3 first beta-propeller domain
WD40_Prp19	PF24814	237 - 383	6.3e-16	Prp19 WD40 domain
Beta-prop_WDR3_2nd	PF25172	237 - 382	9.1e-10	WDR3 second beta-propeller domain
WD40_CDC20-Fz	PF24807	238 - 383	1.1e-09	CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	238 - 383	2.6e-07	WDR36/Utp21 second beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	239 - 383	8e-06	TEP-1 second beta-propeller
Beta-prop_THOC3	PF25174	241 - 383	4.2e-14	THOC3 beta-propeller domain
WD40_Gbeta	PF25391	242 - 382	7.4e-07	G protein beta WD-40 repeat protein
Beta-prop_EML_2	PF23414	243 - 383	5e-07	Echinoderm microtubule-associated protein second beta-propeller
WD40_MABP1-WDR62_2nd	PF24782	273 - 383	7.1e-07	MABP1/WDR62 second WD40 domain
Beta-prop_WDR3_1st	PF25173	314 - 383	8.5e-09	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	314 - 383	3.8e-14	WDR5 beta-propeller domain
WD40_WDHD1_1st	PF24817	317 - 383	1.8e-06	WDHD1 first WD40 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000943)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G29860
fragaria_vesca	FvH4_2g39120 FvH4_2g39120 FvH4_2g39120 FvH4_2g39120 FvH4_2g39120 FvH4_2g39120 FvH4_2g39120
malus_domestica	MD08G1017100.v1.1 MD15G1016100.v1.1 MD15G1017900.v1.1 MD15G1018000.v1.1
prunus_persica	Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1
pyrus_communis	pycom08g01490 pycom15g01390
rosa_chinensis	RchiOBHm_Chr6g0272131 RchiOBHm_Chr6g0302231
rosa_laevigata	RLG00000011183 RLG00000013699
rosa_multiflora	Rmu_sc0013301.1_g000007 Rmu_sc0027601.1_g000001
rosa_roxburghii	Rroxscaffold_7G00165940 Rroxscaffold_7G00196580
rosa_rugosa	Rorug06G0065900 Rorug06G0313400.1
rosa_samantha	Rh6AG183100 Rh6BG184000 Rh6BG476300 Rh6CG184400 Rh6DG176100 Rh6DG425800
rosa_wichuraiana	Rw4G012510 Rw6G015760 Rw6G036890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	744
AccII	CGCG	1 cut(s)	57
AciI	CCGC	4 cut(s)	23, 38, 55, 987
AclI	AACGTT	1 cut(s)	462
AclWI	GGATC	3 cut(s)	582, 881, 894
AcoI	YGGCCR	1 cut(s)	20
AcsI	RAATTY	2 cut(s)	942, 975
AcuI	CTGAAG	1 cut(s)	1101
AdeI	CACNNNGTG	1 cut(s)	1006
AfaI	GTAC	2 cut(s)	214, 901
AfiI	CCNNNNNNNGG	6 cut(s)	37, 241, 744, 786, 896, 993
AflIII	ACRYGT	2 cut(s)	902, 1067
AgeI	ACCGGT	1 cut(s)	107
AgsI	TTSAA	7 cut(s)	272, 467, 500, 674, 894, 948, 1051
AjnI	CCWGG	3 cut(s)	234, 287, 937
AleI	CACNNNNGTG	1 cut(s)	1130
AluBI	AGCT	5 cut(s)	170, 615, 854, 1091, 1102
AluI	AGCT	5 cut(s)	170, 615, 854, 1091, 1102
Alw26I	GTCTC	2 cut(s)	10, 450
AlwI	GGATC	3 cut(s)	582, 881, 894
AlwNI	CAGNNNCTG	1 cut(s)	854
Ama87I	CYCGRG	1 cut(s)	631
AoxI	GGCC	3 cut(s)	20, 49, 353
ApeKI	GCWGC	4 cut(s)	6, 185, 615, 854
ApoI	RAATTY	2 cut(s)	942, 975
AsiGI	ACCGGT	1 cut(s)	107
Asp700I	GAANNNNTTC	1 cut(s)	804
AspLEI	GCGC	2 cut(s)	59, 494
AspS9I	GGNCC	5 cut(s)	28, 49, 70, 423, 997
AsuC2I	CCSGG	3 cut(s)	632, 633, 765
AsuHPI	GGTGA	4 cut(s)	97, 358, 675, 1049
AvaI	CYCGRG	1 cut(s)	631
AvaII	GGWCC	4 cut(s)	28, 70, 423, 997
BaeI	ACNNNNGTAYC	2 cut(s)	891, 924
BamHI	GGATCC	1 cut(s)	886
BauI	CACGAG	1 cut(s)	372
BbsI	GAAGAC	1 cut(s)	1128
BbvI	GCAGC	4 cut(s)	18, 197, 602, 841
BccI	CCATC	7 cut(s)	113, 239, 392, 694, 725, 818, 962
BceAI	ACGGC	3 cut(s)	35, 617, 1061
BciT130I	CCWGG	3 cut(s)	236, 289, 939
BciVI	GTATCC	1 cut(s)	133
BclI	TGATCA	1 cut(s)	909
BcnI	CCSGG	3 cut(s)	632, 633, 765
BcoDI	GTCTC	2 cut(s)	10, 450
BfaI	CTAG	1 cut(s)	443
BfmI	CTRYAG	3 cut(s)	537, 585, 855
BfoI	RGCGCY	1 cut(s)	495
BfuI	GTATCC	1 cut(s)	133
BisI	GCNGC	5 cut(s)	7, 23, 186, 616, 855
BlsI	GCNGC	5 cut(s)	8, 24, 187, 617, 856
Bme1390I	CCNGG	6 cut(s)	236, 289, 632, 633, 765, 939
Bme18I	GGWCC	4 cut(s)	28, 70, 423, 997
BmeT110I	CYCGRG	1 cut(s)	631
BmgT120I	GGNCC	5 cut(s)	28, 49, 70, 423, 997
BmiI	GGNNCC	8 cut(s)	30, 50, 424, 628, 736, 771, 888, 998
BmrFI	CCNGG	6 cut(s)	236, 289, 632, 633, 765, 939
BmrI	ACTGGG	1 cut(s)	26
BmsI	GCATC	3 cut(s)	160, 493, 1118
BmuI	ACTGGG	1 cut(s)	26
BoxI	GACNNNNGTC	1 cut(s)	782
BpiI	GAAGAC	1 cut(s)	1128
Bpu10I	CCTNAGC	1 cut(s)	480
BpuEI	CTTGAG	1 cut(s)	495
BpuMI	CCSGG	3 cut(s)	632, 633, 765
BsaAI	YACGTR	1 cut(s)	903
BsaJI	CCNNGG	7 cut(s)	235, 287, 342, 630, 631, 645, 764
BsaWI	WCCGGW	1 cut(s)	107
BsaXI	ACNNNNNCTCC	2 cut(s)	44, 74
Bsc4I	CCNNNNNNNGG	6 cut(s)	37, 241, 744, 786, 896, 993
Bse118I	RCCGGY	1 cut(s)	107
Bse1I	ACTGG	2 cut(s)	32, 803
Bse3DI	GCAATG	1 cut(s)	841
BseBI	CCWGG	3 cut(s)	236, 289, 939
BseDI	CCNNGG	7 cut(s)	235, 287, 342, 630, 631, 645, 764
BseGI	GGATG	6 cut(s)	82, 250, 403, 484, 736, 829
BseLI	CCNNNNNNNGG	6 cut(s)	37, 241, 744, 786, 896, 993
BseMI	GCAATG	1 cut(s)	841
BseMII	CTCAG	5 cut(s)	35, 119, 471, 549, 861
BseNI	ACTGG	2 cut(s)	32, 803
BseRI	GAGGAG	1 cut(s)	1146
BseX3I	CGGCCG	1 cut(s)	20
BseXI	GCAGC	4 cut(s)	18, 197, 602, 841
BseYI	CCCAGC	3 cut(s)	170, 488, 993
Bsh1236I	CGCG	1 cut(s)	57
Bsh1285I	CGRYCG	1 cut(s)	23
BshFI	GGCC	3 cut(s)	22, 51, 355
BshTI	ACCGGT	1 cut(s)	107
BsiEI	CGRYCG	1 cut(s)	23
BsiHKCI	CYCGRG	1 cut(s)	631
BsiSI	CCGG	4 cut(s)	26, 108, 632, 764
BslFI	GGGAC	3 cut(s)	262, 436, 1010
BslI	CCNNNNNNNGG	6 cut(s)	37, 241, 744, 786, 896, 993
BsmAI	GTCTC	2 cut(s)	10, 450
BsmBI	CGTCTC	1 cut(s)	10
BsmFI	GGGAC	3 cut(s)	262, 436, 1010
BsmI	GAATGC	1 cut(s)	677
BsnI	GGCC	3 cut(s)	22, 51, 355
BsoBI	CYCGRG	1 cut(s)	631
Bsp143I	GATC	3 cut(s)	574, 886, 909
BspACI	CCGC	4 cut(s)	23, 38, 55, 987
BspANI	GGCC	3 cut(s)	22, 51, 355
BspCNI	CTCAG	5 cut(s)	36, 120, 472, 550, 860
BspFNI	CGCG	1 cut(s)	57
BspLI	GGNNCC	8 cut(s)	30, 50, 424, 628, 736, 771, 888, 998
BspMAI	CTGCAG	1 cut(s)	859
BspPI	GGATC	3 cut(s)	582, 881, 894
BsrDI	GCAATG	1 cut(s)	841
BsrFI	RCCGGY	1 cut(s)	107
BsrI	ACTGG	2 cut(s)	32, 803
BssAI	RCCGGY	1 cut(s)	107
BssECI	CCNNGG	7 cut(s)	235, 287, 342, 630, 631, 645, 764
BssMI	GATC	3 cut(s)	574, 886, 909
BssSI	CACGAG	1 cut(s)	372
BssT1I	CCWWGG	2 cut(s)	342, 645
Bst2BI	CACGAG	1 cut(s)	372
Bst2UI	CCWGG	3 cut(s)	236, 289, 939
Bst4CI	ACNGT	5 cut(s)	160, 253, 452, 538, 1132
Bst6I	CTCTTC	1 cut(s)	344
BstBAI	YACGTR	1 cut(s)	903
BstC8I	GCNNGC	2 cut(s)	11, 991
BstDEI	CTNAG	6 cut(s)	44, 128, 480, 558, 809, 847
BstENI	CCTNNNNNAGG	1 cut(s)	784
BstF5I	GGATG	6 cut(s)	82, 250, 403, 484, 736, 829
BstFNI	CGCG	1 cut(s)	57
BstH2I	RGCGCY	1 cut(s)	495
BstHHI	GCGC	2 cut(s)	59, 494
BstKTI	GATC	3 cut(s)	577, 889, 912
BstMAI	GTCTC	2 cut(s)	10, 450
BstMBI	GATC	3 cut(s)	574, 886, 909
BstMCI	CGRYCG	1 cut(s)	23
BstMWI	GCNNNNNNNGC	2 cut(s)	19, 674
BstNI	CCWGG	3 cut(s)	236, 289, 939
BstPAI	GACNNNNGTC	1 cut(s)	782
BstSCI	CCNGG	6 cut(s)	234, 287, 630, 631, 763, 937
BstSFI	CTRYAG	3 cut(s)	537, 585, 855
BstUI	CGCG	1 cut(s)	57
BstV1I	GCAGC	4 cut(s)	18, 197, 602, 841
BstV2I	GAAGAC	1 cut(s)	1128
BstX2I	RGATCY	2 cut(s)	574, 886
BstYI	RGATCY	2 cut(s)	574, 886
BstZI	CGGCCG	1 cut(s)	20
BsuI	GTATCC	1 cut(s)	133
BsuRI	GGCC	3 cut(s)	22, 51, 355
BtsCI	GGATG	6 cut(s)	82, 250, 403, 484, 736, 829
BtsIMutI	CAGTG	4 cut(s)	39, 426, 534, 1137
Cac8I	GCNNGC	2 cut(s)	11, 991
CaiI	CAGNNNCTG	1 cut(s)	854
CfoI	GCGC	2 cut(s)	59, 494
Cfr10I	RCCGGY	1 cut(s)	107
Cfr13I	GGNCC	5 cut(s)	28, 49, 70, 423, 997
Cfr9I	CCCGGG	1 cut(s)	631
Csp6I	GTAC	2 cut(s)	213, 900
CspAI	ACCGGT	1 cut(s)	107
CviAII	CATG	6 cut(s)	67, 191, 664, 739, 831, 1061
CviQI	GTAC	2 cut(s)	213, 900
DdeI	CTNAG	6 cut(s)	44, 128, 480, 558, 809, 847
DpnI	GATC	3 cut(s)	576, 888, 911
DpnII	GATC	3 cut(s)	574, 886, 909
DraIII	CACNNNGTG	1 cut(s)	1006
EaeI	YGGCCR	1 cut(s)	20
EagI	CGGCCG	1 cut(s)	20
Eam1104I	CTCTTC	1 cut(s)	344
EarI	CTCTTC	1 cut(s)	344
EclXI	CGGCCG	1 cut(s)	20
Eco130I	CCWWGG	2 cut(s)	342, 645
Eco147I	AGGCCT	1 cut(s)	355
Eco47I	GGWCC	4 cut(s)	28, 70, 423, 997
Eco52I	CGGCCG	1 cut(s)	20
Eco57I	CTGAAG	1 cut(s)	1101
Eco88I	CYCGRG	1 cut(s)	631
EcoNI	CCTNNNNNAGG	1 cut(s)	784
EcoRII	CCWGG	3 cut(s)	234, 287, 937
EcoT14I	CCWWGG	2 cut(s)	342, 645
ErhI	CCWWGG	2 cut(s)	342, 645
Esp3I	CGTCTC	1 cut(s)	10
FaeI	CATG	6 cut(s)	70, 194, 667, 742, 834, 1064
FaqI	GGGAC	3 cut(s)	262, 436, 1010
FatI	CATG	6 cut(s)	66, 190, 663, 738, 830, 1060
FbaI	TGATCA	1 cut(s)	909
Fnu4HI	GCNGC	5 cut(s)	7, 23, 186, 616, 855
FokI	GGATG	6 cut(s)	69, 257, 410, 471, 743, 836
Fsp4HI	GCNGC	5 cut(s)	7, 23, 186, 616, 855
FspBI	CTAG	1 cut(s)	443
GlaI	GCGC	2 cut(s)	58, 493
GluI	GCNGC	5 cut(s)	7, 23, 186, 616, 855
GsaI	CCCAGC	3 cut(s)	174, 492, 997
HaeII	RGCGCY	1 cut(s)	495
HaeIII	GGCC	3 cut(s)	22, 51, 355
HapII	CCGG	4 cut(s)	26, 108, 632, 764
HhaI	GCGC	2 cut(s)	59, 494
Hin1II	CATG	6 cut(s)	70, 194, 667, 742, 834, 1064
Hin6I	GCGC	2 cut(s)	57, 492
HinP1I	GCGC	2 cut(s)	57, 492
HinfI	GANTC	4 cut(s)	292, 472, 692, 702
HpaII	CCGG	4 cut(s)	26, 108, 632, 764
HphI	GGTGA	4 cut(s)	97, 358, 675, 1049
Hpy166II	GTNNAC	3 cut(s)	105, 335, 379
Hpy188I	TCNGA	7 cut(s)	45, 118, 156, 559, 691, 799, 1120
Hpy188III	TCNNGA	6 cut(s)	310, 395, 443, 512, 849, 913
Hpy8I	GTNNAC	3 cut(s)	105, 335, 379
HpyAV	CCTTC	5 cut(s)	96, 266, 405, 461, 900
HpyCH4III	ACNGT	5 cut(s)	160, 253, 452, 538, 1132
HpyCH4IV	ACGT	3 cut(s)	462, 902, 1067
HpyCH4V	TGCA	8 cut(s)	178, 185, 599, 663, 834, 857, 983, 1064
HpyF10VI	GCNNNNNNNGC	2 cut(s)	19, 674
HpyF3I	CTNAG	6 cut(s)	44, 128, 480, 558, 809, 847
HpySE526I	ACGT	3 cut(s)	462, 902, 1067
Hsp92II	CATG	6 cut(s)	70, 194, 667, 742, 834, 1064
HspAI	GCGC	2 cut(s)	57, 492
Ksp22I	TGATCA	1 cut(s)	909
Kzo9I	GATC	3 cut(s)	574, 886, 909
LmnI	GCTCC	2 cut(s)	547, 851
Lsp1109I	GCAGC	4 cut(s)	18, 197, 602, 841
LweI	GCATC	3 cut(s)	160, 493, 1118
MaeI	CTAG	1 cut(s)	443
MaeII	ACGT	3 cut(s)	462, 902, 1067
MaeIII	GTNAC	2 cut(s)	301, 532
MalI	GATC	3 cut(s)	576, 888, 911
MboI	GATC	3 cut(s)	574, 886, 909
MboII	GAAGA	5 cut(s)	361, 446, 512, 633, 1133
MflI	RGATCY	2 cut(s)	574, 886
MluCI	AATT	4 cut(s)	518, 921, 942, 975
MlyI	GAGTC	1 cut(s)	286
MroXI	GAANNNNTTC	1 cut(s)	804
MseI	TTAA	4 cut(s)	255, 387, 579, 1165
MslI	CAYNNNNRTG	1 cut(s)	1130
MspA1I	CMGCKG	3 cut(s)	170, 989, 1091
MspI	CCGG	4 cut(s)	26, 108, 632, 764
MspR9I	CCNGG	6 cut(s)	236, 289, 632, 633, 765, 939
Mva1269I	GAATGC	1 cut(s)	677
MvaI	CCWGG	3 cut(s)	236, 289, 939
MvnI	CGCG	1 cut(s)	57
MwoI	GCNNNNNNNGC	2 cut(s)	19, 674
NciI	CCSGG	3 cut(s)	632, 633, 765
NdeII	GATC	3 cut(s)	574, 886, 909
NlaIII	CATG	6 cut(s)	70, 194, 667, 742, 834, 1064
NlaIV	GGNNCC	8 cut(s)	30, 50, 424, 628, 736, 771, 888, 998
NmuCI	GTSAC	1 cut(s)	532
OliI	CACNNNNGTG	1 cut(s)	1130
PceI	AGGCCT	1 cut(s)	355
PctI	GAATGC	1 cut(s)	677
PdmI	GAANNNNTTC	1 cut(s)	804
PfeI	GAWTC	3 cut(s)	472, 692, 702
PflFI	GACNNNGTC	1 cut(s)	295
PflMI	CCANNNNNTGG	1 cut(s)	744
PinAI	ACCGGT	1 cut(s)	107
PkrI	GCNGC	5 cut(s)	8, 24, 187, 617, 856
PleI	GAGTC	1 cut(s)	286
PpsI	GAGTC	1 cut(s)	286
Ppu21I	YACGTR	1 cut(s)	903
PshAI	GACNNNNGTC	1 cut(s)	782
Psp1406I	AACGTT	1 cut(s)	462
Psp6I	CCWGG	3 cut(s)	234, 287, 937
PspFI	CCCAGC	3 cut(s)	170, 488, 993
PspGI	CCWGG	3 cut(s)	234, 287, 937
PspN4I	GGNNCC	8 cut(s)	30, 50, 424, 628, 736, 771, 888, 998
PspPI	GGNCC	5 cut(s)	28, 49, 70, 423, 997
PstI	CTGCAG	1 cut(s)	859
PstNI	CAGNNNCTG	1 cut(s)	854
PsuI	RGATCY	2 cut(s)	574, 886
PsyI	GACNNNGTC	1 cut(s)	295
PvuII	CAGCTG	2 cut(s)	170, 1091
RsaI	GTAC	2 cut(s)	214, 901
RsaNI	GTAC	2 cut(s)	213, 900
RseI	CAYNNNNRTG	1 cut(s)	1130
SaqAI	TTAA	4 cut(s)	255, 387, 579, 1165
SatI	GCNGC	5 cut(s)	7, 23, 186, 616, 855
Sau3AI	GATC	3 cut(s)	574, 886, 909
Sau96I	GGNCC	5 cut(s)	28, 49, 70, 423, 997
SchI	GAGTC	1 cut(s)	286
ScrFI	CCNGG	6 cut(s)	236, 289, 632, 633, 765, 939
SfaNI	GCATC	3 cut(s)	160, 493, 1118
SfcI	CTRYAG	3 cut(s)	537, 585, 855
SinI	GGWCC	4 cut(s)	28, 70, 423, 997
SmaI	CCCGGG	1 cut(s)	633
SmiMI	CAYNNNNRTG	1 cut(s)	1130
SmlI	CTYRAG	1 cut(s)	510
SmoI	CTYRAG	1 cut(s)	510
Sse9I	AATT	4 cut(s)	518, 921, 942, 975
SseBI	AGGCCT	1 cut(s)	355
SsiI	CCGC	4 cut(s)	23, 38, 55, 987
SspMI	CTAG	1 cut(s)	443
StuI	AGGCCT	1 cut(s)	355
StyD4I	CCNGG	6 cut(s)	234, 287, 630, 631, 763, 937
StyI	CCWWGG	2 cut(s)	342, 645
TaaI	ACNGT	5 cut(s)	160, 253, 452, 538, 1132
TaiI	ACGT	3 cut(s)	465, 905, 1070
TaqI	TCGA	2 cut(s)	961, 1159
TasI	AATT	4 cut(s)	518, 921, 942, 975
TatI	WGTACW	1 cut(s)	212
TauI	GCSGC	1 cut(s)	25
TfiI	GAWTC	3 cut(s)	472, 692, 702
Tru1I	TTAA	4 cut(s)	255, 387, 579, 1165
Tru9I	TTAA	4 cut(s)	255, 387, 579, 1165
TscAI	CASTG	4 cut(s)	39, 433, 541, 1137
TseFI	GTSAC	1 cut(s)	532
TseI	GCWGC	4 cut(s)	6, 185, 615, 854
Tsp45I	GTSAC	1 cut(s)	532
TspDTI	ATGAA	5 cut(s)	71, 102, 447, 531, 782
TspGWI	ACGGA	2 cut(s)	52, 285
TspMI	CCCGGG	1 cut(s)	631
TspRI	CASTG	4 cut(s)	39, 433, 541, 1137
Tth111I	GACNNNGTC	1 cut(s)	295
Van91I	CCANNNNNTGG	1 cut(s)	744
VpaK11BI	GGWCC	4 cut(s)	28, 70, 423, 997
XagI	CCTNNNNNAGG	1 cut(s)	784
XapI	RAATTY	2 cut(s)	942, 975
XbaI	TCTAGA	1 cut(s)	442
XcmI	CCANNNNNNNNNTGG	1 cut(s)	242
XmaI	CCCGGG	1 cut(s)	631
XmnI	GAANNNNTTC	1 cut(s)	804
XspI	CTAG	1 cut(s)	443

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.