MD08G1044200.v1.1

Far upstream element-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0009579
Physical Location & Seq
Forward (+)
3385952 .. 3391767
5816 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1044200.v1.1.491

Sequence Viewer

Length: 2031 bp
ATGGCCGACGAAGCCCAATACTCATCCGGTCCTGATTCAGGGAACAAGCGGAAGTACGAGGAGCAAACGCCGCCGTCCACTCGCAGAGTCACCGGTTTCTCGGCCCCCATCAAGTCATCGTCCCCCGACTCTGTGCCGACGTCGTACAGCAGCGTTCCTCCGCCCATGGAAGATTTCCAGCTGGCCAAGCAGAAGGCGCAGGAGATCGCCGCTCGGTTGTTGAACGGCGGCGATGCTAAGCGTGCTAGGGTTGAGAATGGTGGTTCCGACTCTTTTGACAAGGGCTTTAGCTCTGGTCCGCCAGATCCAAAGCCTCACTTCTCAAACCCAGCTCCTTCGTCGATCCCTGTATCTTATGGTGGGTTCGTGGGACCAAGCAAAAAGATTGAGGTTCCCAATGGCAGGGTTGGTGTCATAATTGGTAAAGGTGGGGAGACTATCAAGTATCTACAGACTCAGTCAGGCGCTAAGATTCAAGTTACCCGAGATTCTGATGCAGACCTCAATTTTCCAACTAGGATGGTGGAGCTCATGGGTACTCCGGAGCAGATTGCGAAGGCCGAGCACTTGATAAATGATGTTCTTGCTGAGGCTGAATCAGGAGGTCCTGCTGTAGCTTCTCGAAGGTTAACTGTACATACCGGTGGTGAACAATATGTTACGAAAATTCCTAACAACAAGGTTGGGCTTGTAATTGGTAAAGGAGGTGAAACAATAAAAAGTATGCAAGCTAGGTCTGGAGCTCGTATCCAGGTTATTCCCTTGCATCTGCCACCTGGTGACCCATCAACAGAAAGGACGCTACAGATTGATGGTACTACTGAACAAATTGAAGCTGCAAAACAGTTGGTAGCTGAAGTTATCAGTGAGAACCGTGTTAGAAATCCAGCAACGTCTGGAGGATACTCTCAGCAAGGTTATCAAGCACGACCACCCTCAGGATGGGCTCCTTCTGGTCCCTCAATGCAACAACCTAGTTATGGATACGGACAGTCTGGAGGGTATTCTGCCCCACCACATCAGTATAATGCGTCTCAGCCTTCTTACCCAGGCTATCCTCCTCAACCAACATCTGGTGGCTATCCGTCCAGTTGGGACCAGTCGGCTGCTGCTCCAACTCAGCAGACGTCCCAGGGTGGTGGTTATGATTACTATGGCCAGCAACAACCTTCACATCAACAGCAAAATCCTGGAGGTCCTGCTGCTCCTGCTGATGGTGCTGGTTACAATTATGGCCCGCCGCCAGTTTCAGGCTATAATCAACAAGGACAAGGGTATGCTCAAGGATATCCTGCACCACCTCAATCTGGGTATGGTCAGCCACCGGCATATGATCAACAGCAGGGTTATTCTGCAGCAGGCTATGGTAATGTGAGTAACCCACCTCAAGATGGGCATACACCCTCTTATGGATCTCAGGGGGATTCAGCTCAGGCGCCTCCTGCTCAGCAAGGATACAATGCCGGTCAACAGCCTAGCCCTAACCCTGCTGGTTATCCACCCCAAGGTTCTACTCAGCCAGGTTATGGAGCACCCCCAACTTCTCAAACTGGTTATGGAAGCCAACCACCGGCTCAGCCTGCATATGGAGCTGGATATGGAGCACCACCAGCACAAAAGCCTCCTGCAAATCCTGCAGTTTATGGGCAAACTGCTCAATCCCCCAGCACTCCAGGAGGCTATGGCCAGCCAGCTCCTGTGCAGCCAGGATACCCGCATTCTCAGCCTCCAGCTTCTGGATATGCTCAGCCAGATCCTGTTACCCGTCCCCCTACATCTGGCTATGGTGCTGCAGCACCACAGACCGGGTATGGGCCTCCATCCTATGGCGCACCACCAGCTGGCCAGCCAGGATATGGGCAGGTGCCACCACCCTACAACGCATCTTATGGTGCTGGATATGCACAACCTCCTCCTTACTCTGCTGATACCAGCACAAATGGGAACGCTCGTGTGACCTATGATGCTCCGCCACCTGCTTCACAGACTGCTCCCCCAAGTGGAGTTGCCAAAACTTCCCCTCAGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001505 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003730 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006139 GO:0006401 GO:0006402 GO:0006417 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010033 GO:0010468 GO:0010494 GO:0010556 GO:0010558 GO:0010586 GO:0010604 GO:0010605 GO:0010608 GO:0010629 GO:0010984 GO:0010985 GO:0010988 GO:0010989 GO:0016070 GO:0016071 GO:0017091 GO:0017148 GO:0019219 GO:0019222 GO:0019439 GO:0030425 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031329 GO:0031331 GO:0031974 GO:0031981 GO:0032268 GO:0032269 GO:0032991 GO:0034097 GO:0034248 GO:0034249 GO:0034641 GO:0034655 GO:0034660 GO:0035770 GO:0035925 GO:0036464 GO:0036477 GO:0042221 GO:0042995 GO:0043005 GO:0043025 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043487 GO:0043488 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044297 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044463 GO:0044464 GO:0045019 GO:0045428 GO:0045935 GO:0046483 GO:0046700 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050779 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051173 GO:0051239 GO:0051241 GO:0051246 GO:0051248 GO:0051252 GO:0051254 GO:0051716 GO:0060255 GO:0061013 GO:0061014 GO:0061157 GO:0061158 GO:0065007 GO:0065008 GO:0070013 GO:0070887 GO:0071310 GO:0071345 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0097447 GO:0097458 GO:0120025 GO:0120038 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1903311 GO:1903313 GO:1903426 GO:1903427 GO:1904406 GO:1990904 GO:2000112 GO:2000113 GO:2000377 GO:2000378 GO:2000628
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KH_1

Protein Analysis

677

Amino Acids

69.64

Weight (kDa)

6.27

Isoelectric Point (pI)

59.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KH_1 PF00013 127 - 193 5.5e-18 KH domain
KH_1 PF00013 222 - 285 5.3e-19 KH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1852, 1984
AatII GACGTC 2 cut(s) 143, 1130
Acc36I ACCTGC 2 cut(s) 1852, 1984
AccB1I GGYRCC 2 cut(s) 1435, 1864
AccB7I CCANNNNNTGG 6 cut(s) 1073, 1526, 1736, 1826, 1841, 1856
AccBSI CCGCTC 1 cut(s) 212
AccIII TCCGGA 1 cut(s) 541
AclWI GGATC 4 cut(s) 299, 337, 1420, 1748
AcoI YGGCCR 5 cut(s) 3, 183, 1156, 1684, 1843
AcsI RAATTY 1 cut(s) 666
AcuI CTGAAG 1 cut(s) 876
AcyI GRCGYC 3 cut(s) 140, 1127, 1436
AdeI CACNNNGTG 1 cut(s) 779
AfaI GTAC 5 cut(s) 56, 146, 538, 636, 817
AgeI ACCGGT 2 cut(s) 92, 641
AgsI TTSAA 3 cut(s) 223, 476, 833
AjnI CCWGG 9 cut(s) 750, 775, 1048, 1131, 1189, 1519, 1672, 1705, 1849
Alw21I GWGCWC 5 cut(s) 531, 567, 745, 1534, 1606
Alw26I GTCTC 2 cut(s) 428, 1038
AlwI GGATC 4 cut(s) 299, 337, 1420, 1748
AlwNI CAGNNNCTG 3 cut(s) 1697, 1736, 1757
Ama87I CYCGRG 1 cut(s) 483
Aor13HI TCCGGA 1 cut(s) 541
AoxI GGCC 9 cut(s) 3, 102, 183, 558, 1156, 1234, 1684, 1814, 1843
ApeKI GCWGC 9 cut(s) 150, 836, 1106, 1109, 1202, 1355, 1702, 1790, 1793
ApoI RAATTY 1 cut(s) 666
AsiGI ACCGGT 2 cut(s) 92, 641
AspLEI GCGC 4 cut(s) 199, 467, 1438, 1832
AsuC2I CCSGG 1 cut(s) 1807
AsuHPI GGTGA 4 cut(s) 82, 659, 719, 791
AvaI CYCGRG 1 cut(s) 483
AvaII GGWCC 7 cut(s) 29, 296, 371, 605, 956, 1096, 1196
AxyI CCTNAGG 1 cut(s) 937
BalI TGGCCA 4 cut(s) 185, 1158, 1686, 1845
BanI GGYRCC 2 cut(s) 1435, 1864
BanII GRGCYC 3 cut(s) 531, 745, 949
BauI CACGAG 1 cut(s) 1950
Bbv12I GWGCWC 5 cut(s) 531, 567, 745, 1534, 1606
BbvCI CCTCAGC 1 cut(s) 588
BbvI GCAGC 9 cut(s) 162, 823, 1093, 1096, 1189, 1367, 1714, 1777, 1805
BccI CCATC 8 cut(s) 116, 514, 793, 806, 936, 1208, 1385, 1828
BceAI ACGGC 2 cut(s) 58, 241
BciT130I CCWGG 9 cut(s) 752, 777, 1050, 1133, 1191, 1521, 1674, 1707, 1851
BciVI GTATCC 5 cut(s) 758, 896, 977, 1448, 1703
BclI TGATCA 1 cut(s) 1333
BcnI CCSGG 1 cut(s) 1807
BcoDI GTCTC 2 cut(s) 428, 1038
BfaI CTAG 5 cut(s) 246, 516, 732, 975, 1476
BfmI CTRYAG 6 cut(s) 449, 612, 803, 1353, 1635, 1791
BfoI RGCGCY 2 cut(s) 468, 1439
BfuAI ACCTGC 2 cut(s) 1852, 1984
BfuI GTATCC 5 cut(s) 758, 896, 977, 1448, 1703
BlpI GCTNAGC 4 cut(s) 237, 1446, 1575, 1746
Bme18I GGWCC 7 cut(s) 29, 296, 371, 605, 956, 1096, 1196
BmeT110I CYCGRG 1 cut(s) 483
BmiI GGNNCC 9 cut(s) 105, 265, 372, 393, 948, 958, 1097, 1437, 1866
BmsI GCATC 5 cut(s) 223, 484, 775, 1892, 1954
BpmI CTGGAG 6 cut(s) 759, 918, 1017, 1212, 1656, 1713
Bpu10I CCTNAGC 2 cut(s) 588, 1431
Bpu1102I GCTNAGC 4 cut(s) 237, 1446, 1575, 1746
BpuEI CTTGAG 2 cut(s) 1266, 1371
BpuMI CCSGG 1 cut(s) 1807
BsaHI GRCGYC 3 cut(s) 140, 1127, 1436
BsaJI CCNNGG 5 cut(s) 165, 1048, 1131, 1132, 1504
BsaWI WCCGGW 4 cut(s) 26, 92, 541, 641
Bse118I RCCGGY 5 cut(s) 92, 641, 1324, 1463, 1570
Bse1I ACTGG 4 cut(s) 1089, 1099, 1244, 1555
Bse21I CCTNAGG 1 cut(s) 937
BseAI TCCGGA 1 cut(s) 541
BseBI CCWGG 9 cut(s) 752, 777, 1050, 1133, 1191, 1521, 1674, 1707, 1851
BseDI CCNNGG 5 cut(s) 165, 1048, 1131, 1132, 1504
BseGI GGATG 4 cut(s) 23, 525, 947, 1820
BseNI ACTGG 4 cut(s) 1089, 1099, 1244, 1555
BseRI GAGGAG 3 cut(s) 74, 1050, 1902
BseXI GCAGC 9 cut(s) 162, 823, 1093, 1096, 1189, 1367, 1714, 1777, 1805
BseYI CCCAGC 2 cut(s) 328, 1664
BsgI GTGCAG 2 cut(s) 1278, 1721
BshFI GGCC 9 cut(s) 5, 104, 185, 560, 1158, 1236, 1686, 1816, 1845
BshNI GGYRCC 2 cut(s) 1435, 1864
BshTI ACCGGT 2 cut(s) 92, 641
BsiHKAI GWGCWC 5 cut(s) 531, 567, 745, 1534, 1606
BsiHKCI CYCGRG 1 cut(s) 483
BsiSI CCGG 8 cut(s) 27, 93, 542, 642, 1325, 1464, 1571, 1806
BslFI GGGAC 6 cut(s) 106, 384, 942, 1109, 1114, 1752
BsmAI GTCTC 2 cut(s) 428, 1038
BsmBI CGTCTC 1 cut(s) 1038
BsmFI GGGAC 6 cut(s) 106, 384, 942, 1109, 1114, 1752
BsmI GAATGC 1 cut(s) 1717
BsnI GGCC 9 cut(s) 5, 104, 185, 560, 1158, 1236, 1686, 1816, 1845
BsoBI CYCGRG 1 cut(s) 483
Bsp1286I GDGCHC 6 cut(s) 531, 567, 745, 949, 1534, 1606
Bsp13I TCCGGA 1 cut(s) 541
Bsp1407I TGTACA 1 cut(s) 634
Bsp143I GATC 6 cut(s) 204, 304, 342, 1333, 1412, 1753
Bsp1720I GCTNAGC 4 cut(s) 237, 1446, 1575, 1746
Bsp19I CCATGG 1 cut(s) 165
BspANI GGCC 9 cut(s) 5, 104, 185, 560, 1158, 1236, 1686, 1816, 1845
BspEI TCCGGA 1 cut(s) 541
BspLI GGNNCC 9 cut(s) 105, 265, 372, 393, 948, 958, 1097, 1437, 1866
BspMAI CTGCAG 3 cut(s) 1357, 1639, 1795
BspMI ACCTGC 2 cut(s) 1852, 1984
BspPI GGATC 4 cut(s) 299, 337, 1420, 1748
BspT107I GGYRCC 2 cut(s) 1435, 1864
BsrBI CCGCTC 1 cut(s) 212
BsrFI RCCGGY 5 cut(s) 92, 641, 1324, 1463, 1570
BsrGI TGTACA 1 cut(s) 634
BsrI ACTGG 4 cut(s) 1089, 1099, 1244, 1555
BssAI RCCGGY 5 cut(s) 92, 641, 1324, 1463, 1570
BssECI CCNNGG 5 cut(s) 165, 1048, 1131, 1132, 1504
BssMI GATC 6 cut(s) 204, 304, 342, 1333, 1412, 1753
BssNI GRCGYC 3 cut(s) 140, 1127, 1436
BssSI CACGAG 1 cut(s) 1950
BssT1I CCWWGG 2 cut(s) 165, 1504
Bst2BI CACGAG 1 cut(s) 1950
Bst2UI CCWGG 9 cut(s) 752, 777, 1050, 1133, 1191, 1521, 1674, 1707, 1851
Bst4CI ACNGT 4 cut(s) 634, 846, 875, 993
BstACI GRCGYC 3 cut(s) 140, 1127, 1436
BstAPI GCANNNNNTGC 1 cut(s) 1634
BstAUI TGTACA 1 cut(s) 634
BstDSI CCRYGG 1 cut(s) 165
BstEII GGTNACC 1 cut(s) 779
BstENI CCTNNNNNAGG 1 cut(s) 36
BstF5I GGATG 4 cut(s) 23, 525, 947, 1820
BstH2I RGCGCY 2 cut(s) 468, 1439
BstHHI GCGC 4 cut(s) 199, 467, 1438, 1832
BstKTI GATC 6 cut(s) 207, 307, 345, 1336, 1415, 1756
BstMAI GTCTC 2 cut(s) 428, 1038
BstMBI GATC 6 cut(s) 204, 304, 342, 1333, 1412, 1753
BstNI CCWGG 9 cut(s) 752, 777, 1050, 1133, 1191, 1521, 1674, 1707, 1851
BstPI GGTNACC 1 cut(s) 779
BstSFI CTRYAG 6 cut(s) 449, 612, 803, 1353, 1635, 1791
BstV1I GCAGC 9 cut(s) 162, 823, 1093, 1096, 1189, 1367, 1714, 1777, 1805
BstX2I RGATCY 3 cut(s) 304, 1412, 1753
BstXI CCANNNNNNTGG 1 cut(s) 1139
BstYI RGATCY 3 cut(s) 304, 1412, 1753
Bsu36I CCTNAGG 1 cut(s) 937
BsuI GTATCC 5 cut(s) 758, 896, 977, 1448, 1703
BsuRI GGCC 9 cut(s) 5, 104, 185, 560, 1158, 1236, 1686, 1816, 1845
BtgI CCRYGG 1 cut(s) 165
BtgZI GCGATG 1 cut(s) 246
BtsCI GGATG 4 cut(s) 23, 525, 947, 1820
BtsIMutI CAGTG 1 cut(s) 871
BveI ACCTGC 2 cut(s) 1852, 1984
CaiI CAGNNNCTG 3 cut(s) 1697, 1736, 1757
CfoI GCGC 4 cut(s) 199, 467, 1438, 1832
Cfr10I RCCGGY 5 cut(s) 92, 641, 1324, 1463, 1570
CseI GACGC 2 cut(s) 808, 1020
CsiI ACCWGGT 1 cut(s) 775
Csp6I GTAC 5 cut(s) 55, 145, 537, 635, 816
CspAI ACCGGT 2 cut(s) 92, 641
CviAII CATG 2 cut(s) 166, 532
CviQI GTAC 5 cut(s) 55, 145, 537, 635, 816
DinI GGCGCC 1 cut(s) 1437
DpnI GATC 6 cut(s) 206, 306, 344, 1335, 1414, 1755
DpnII GATC 6 cut(s) 204, 304, 342, 1333, 1412, 1753
DraIII CACNNNGTG 1 cut(s) 779
EaeI YGGCCR 5 cut(s) 3, 183, 1156, 1684, 1843
EciI GGCGGA 3 cut(s) 150, 288, 1959
Ecl136II GAGCTC 2 cut(s) 529, 743
Eco130I CCWWGG 2 cut(s) 165, 1504
Eco24I GRGCYC 3 cut(s) 531, 745, 949
Eco32I GATATC 1 cut(s) 1289
Eco47I GGWCC 7 cut(s) 29, 296, 371, 605, 956, 1096, 1196
Eco53kI GAGCTC 2 cut(s) 529, 743
Eco57I CTGAAG 1 cut(s) 876
Eco81I CCTNAGG 1 cut(s) 937
Eco88I CYCGRG 1 cut(s) 483
Eco91I GGTNACC 1 cut(s) 779
EcoICRI GAGCTC 2 cut(s) 529, 743
EcoNI CCTNNNNNAGG 1 cut(s) 36
EcoO109I RGGNCCY 2 cut(s) 605, 1196
EcoO65I GGTNACC 1 cut(s) 779
EcoRII CCWGG 9 cut(s) 750, 775, 1048, 1131, 1189, 1519, 1672, 1705, 1849
EcoRV GATATC 1 cut(s) 1289
EcoT14I CCWWGG 2 cut(s) 165, 1504
EcoT38I GRGCYC 3 cut(s) 531, 745, 949
EgeI GGCGCC 1 cut(s) 1437
EheI GGCGCC 1 cut(s) 1437
ErhI CCWWGG 2 cut(s) 165, 1504
Esp3I CGTCTC 1 cut(s) 1038
FaeI CATG 2 cut(s) 169, 535
FalI AAGNNNNNCTT 2 cut(s) 302, 334
FaqI GGGAC 6 cut(s) 106, 384, 942, 1109, 1114, 1752
FatI CATG 2 cut(s) 165, 531
FauI CCCGC 2 cut(s) 1245, 1722
FauNDI CATATG 2 cut(s) 1330, 1585
FbaI TGATCA 1 cut(s) 1333
FokI GGATG 4 cut(s) 10, 532, 954, 1807
FriOI GRGCYC 3 cut(s) 531, 745, 949
FspBI CTAG 5 cut(s) 246, 516, 732, 975, 1476
GlaI GCGC 4 cut(s) 198, 466, 1437, 1831
GsaI CCCAGC 2 cut(s) 332, 1668
GsuI CTGGAG 6 cut(s) 759, 918, 1017, 1212, 1656, 1713
HaeII RGCGCY 2 cut(s) 468, 1439
HaeIII GGCC 9 cut(s) 5, 104, 185, 560, 1158, 1236, 1686, 1816, 1845
HapII CCGG 8 cut(s) 27, 93, 542, 642, 1325, 1464, 1571, 1806
HgaI GACGC 2 cut(s) 808, 1020
HhaI GCGC 4 cut(s) 199, 467, 1438, 1832
Hin1I GRCGYC 3 cut(s) 140, 1127, 1436
Hin1II CATG 2 cut(s) 169, 535
Hin6I GCGC 4 cut(s) 197, 465, 1436, 1830
HinP1I GCGC 4 cut(s) 197, 465, 1436, 1830
HincII GTYRAC 2 cut(s) 630, 1469
HindII GTYRAC 2 cut(s) 630, 1469
HinfI GANTC 9 cut(s) 35, 87, 128, 269, 454, 472, 488, 596, 1424
HpaI GTTAAC 1 cut(s) 630
HpaII CCGG 8 cut(s) 27, 93, 542, 642, 1325, 1464, 1571, 1806
HphI GGTGA 4 cut(s) 82, 659, 719, 791
Hpy166II GTNNAC 4 cut(s) 78, 630, 650, 1469
Hpy188I TCNGA 3 cut(s) 268, 493, 2025
Hpy8I GTNNAC 4 cut(s) 78, 630, 650, 1469
Hpy99I CGWCG 4 cut(s) 11, 142, 145, 343
HpyAV CCTTC 7 cut(s) 187, 345, 550, 618, 960, 1050, 1179
HpyCH4III ACNGT 4 cut(s) 634, 846, 875, 993
HpyCH4IV ACGT 3 cut(s) 140, 893, 1127
HpySE526I ACGT 3 cut(s) 140, 893, 1127
Hsp92I GRCGYC 3 cut(s) 140, 1127, 1436
Hsp92II CATG 2 cut(s) 169, 535
HspAI GCGC 4 cut(s) 197, 465, 1436, 1830
KasI GGCGCC 1 cut(s) 1435
Kpn2I TCCGGA 1 cut(s) 541
Ksp22I TGATCA 1 cut(s) 1333
KspAI GTTAAC 1 cut(s) 630
Kzo9I GATC 6 cut(s) 204, 304, 342, 1333, 1412, 1753
Lsp1109I GCAGC 9 cut(s) 162, 823, 1093, 1096, 1189, 1367, 1714, 1777, 1805
LweI GCATC 5 cut(s) 223, 484, 775, 1892, 1954
MabI ACCWGGT 1 cut(s) 775
MaeI CTAG 5 cut(s) 246, 516, 732, 975, 1476
MaeII ACGT 3 cut(s) 140, 893, 1127
MaeIII GTNAC 8 cut(s) 88, 478, 658, 779, 1223, 1376, 1759, 1954
MalI GATC 6 cut(s) 206, 306, 344, 1335, 1414, 1755
MbiI CCGCTC 1 cut(s) 212
MboI GATC 6 cut(s) 204, 304, 342, 1333, 1412, 1753
MboII GAAGA 1 cut(s) 182
MflI RGATCY 3 cut(s) 304, 1412, 1753
MhlI GDGCHC 6 cut(s) 531, 567, 745, 949, 1534, 1606
MlsI TGGCCA 4 cut(s) 185, 1158, 1686, 1845
MluCI AATT 6 cut(s) 417, 505, 666, 693, 828, 1228
MluNI TGGCCA 4 cut(s) 185, 1158, 1686, 1845
Mly113I GGCGCC 1 cut(s) 1436
MlyI GAGTC 4 cut(s) 96, 122, 263, 448
MmeI TCCRAC 3 cut(s) 291, 536, 1139
Mox20I TGGCCA 4 cut(s) 185, 1158, 1686, 1845
MroI TCCGGA 1 cut(s) 541
MscI TGGCCA 4 cut(s) 185, 1158, 1686, 1845
MseI TTAA 1 cut(s) 629
MslI CAYNNNNRTG 1 cut(s) 642
Msp20I TGGCCA 4 cut(s) 185, 1158, 1686, 1845
MspA1I CMGCKG 2 cut(s) 181, 1841
MspI CCGG 8 cut(s) 27, 93, 542, 642, 1325, 1464, 1571, 1806
Mva1269I GAATGC 1 cut(s) 1717
MvaI CCWGG 9 cut(s) 752, 777, 1050, 1133, 1191, 1521, 1674, 1707, 1851
NarI GGCGCC 1 cut(s) 1436
NciI CCSGG 1 cut(s) 1807
NcoI CCATGG 1 cut(s) 165
NdeI CATATG 2 cut(s) 1330, 1585
NdeII GATC 6 cut(s) 204, 304, 342, 1333, 1412, 1753
NlaIII CATG 2 cut(s) 169, 535
NlaIV GGNNCC 9 cut(s) 105, 265, 372, 393, 948, 958, 1097, 1437, 1866
NmeAIII GCCGAG 2 cut(s) 80, 586
NmuCI GTSAC 3 cut(s) 88, 779, 1954
PaqCI CACCTGC 2 cut(s) 1852, 1984
PasI CCCWGGG 1 cut(s) 1132
PctI GAATGC 1 cut(s) 1717
PfeI GAWTC 5 cut(s) 35, 472, 488, 596, 1424
PflFI GACNNNGTC 1 cut(s) 457
PflMI CCANNNNNTGG 6 cut(s) 1073, 1526, 1736, 1826, 1841, 1856
PfoI TCCNGGA 2 cut(s) 1189, 1672
PinAI ACCGGT 2 cut(s) 92, 641
PleI GAGTC 4 cut(s) 95, 122, 263, 448
PluTI GGCGCC 1 cut(s) 1439
PpsI GAGTC 4 cut(s) 95, 122, 263, 448
PpuMI RGGWCCY 2 cut(s) 605, 1196
Psp124BI GAGCTC 2 cut(s) 531, 745
Psp5II RGGWCCY 2 cut(s) 605, 1196
Psp6I CCWGG 9 cut(s) 750, 775, 1048, 1131, 1189, 1519, 1672, 1705, 1849
PspEI GGTNACC 1 cut(s) 779
PspFI CCCAGC 2 cut(s) 328, 1664
PspGI CCWGG 9 cut(s) 750, 775, 1048, 1131, 1189, 1519, 1672, 1705, 1849
PspN4I GGNNCC 9 cut(s) 105, 265, 372, 393, 948, 958, 1097, 1437, 1866
PspPPI RGGWCCY 2 cut(s) 605, 1196
PstI CTGCAG 3 cut(s) 1357, 1639, 1795
PstNI CAGNNNCTG 3 cut(s) 1697, 1736, 1757
PsuI RGATCY 3 cut(s) 304, 1412, 1753
PsyI GACNNNGTC 1 cut(s) 457
PvuII CAGCTG 2 cut(s) 181, 1841
RsaI GTAC 5 cut(s) 56, 146, 538, 636, 817
RsaNI GTAC 5 cut(s) 55, 145, 537, 635, 816
RseI CAYNNNNRTG 1 cut(s) 642
SacI GAGCTC 2 cut(s) 531, 745
SaqAI TTAA 1 cut(s) 629
Sau3AI GATC 6 cut(s) 204, 304, 342, 1333, 1412, 1753
SchI GAGTC 4 cut(s) 96, 122, 263, 448
SduI GDGCHC 6 cut(s) 531, 567, 745, 949, 1534, 1606
SexAI ACCWGGT 1 cut(s) 775
SfaNI GCATC 5 cut(s) 223, 484, 775, 1892, 1954
SfcI CTRYAG 6 cut(s) 449, 612, 803, 1353, 1635, 1791
SfoI GGCGCC 1 cut(s) 1437
SinI GGWCC 7 cut(s) 29, 296, 371, 605, 956, 1096, 1196
SmiMI CAYNNNNRTG 1 cut(s) 642
SmlI CTYRAG 2 cut(s) 1281, 1386
SmoI CTYRAG 2 cut(s) 1281, 1386
Sse9I AATT 6 cut(s) 417, 505, 666, 693, 828, 1228
SspDI GGCGCC 1 cut(s) 1435
SspMI CTAG 5 cut(s) 246, 516, 732, 975, 1476
SstI GAGCTC 2 cut(s) 531, 745
StyI CCWWGG 2 cut(s) 165, 1504
TaaI ACNGT 4 cut(s) 634, 846, 875, 993
TaiI ACGT 3 cut(s) 143, 896, 1130
TaqI TCGA 2 cut(s) 341, 622
TasI AATT 6 cut(s) 417, 505, 666, 693, 828, 1228
TatI WGTACW 1 cut(s) 634
TauI GCSGC 4 cut(s) 73, 212, 231, 1243
TfiI GAWTC 5 cut(s) 35, 472, 488, 596, 1424
Tru1I TTAA 1 cut(s) 629
Tru9I TTAA 1 cut(s) 629
TscAI CASTG 1 cut(s) 871
TseFI GTSAC 3 cut(s) 88, 779, 1954
TseI GCWGC 9 cut(s) 150, 836, 1106, 1109, 1202, 1355, 1702, 1790, 1793
Tsp45I GTSAC 3 cut(s) 88, 779, 1954
TspGWI ACGGA 2 cut(s) 1002, 1074
TspRI CASTG 1 cut(s) 871
Tth111I GACNNNGTC 1 cut(s) 457
Van91I CCANNNNNTGG 6 cut(s) 1073, 1526, 1736, 1826, 1841, 1856
VpaK11BI GGWCC 7 cut(s) 29, 296, 371, 605, 956, 1096, 1196
XagI CCTNNNNNAGG 1 cut(s) 36
XapI RAATTY 1 cut(s) 666
XcmI CCANNNNNNNNNTGG 2 cut(s) 939, 1853
XspI CTAG 5 cut(s) 246, 516, 732, 975, 1476
ZraI GACGTC 2 cut(s) 141, 1128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.