MD08G1060500.v1.1

CST complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0006351
Physical Location & Seq
Reverse (-)
4793913 .. 4800155
6243 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1060500.v1.1.491

Sequence Viewer

Length: 4098 bp
ATGGAAAATGTGAGAATTCTCACAGTCGCAGAGCTACTCGCGCGCGGCCGTCCCCTCACCGCCACGTCCTCCATCTCTCCCAATTCGAACCAAAAGTCAAAGCTCCCCACGCGCTCCGTTCCCACCCACTCAAACCCTAGCCCCAATCCCAACCCTAGCCCTAAGTTTCTCACTCCTCTCGACCACTTCGCCGTCATCGTCGGAACCCTAGCCCTCCCCACTACCACCGCTGCATCCGATTCTCGGTGCTCCTACAACACTTGCTTCCAATTCTCCGACGGCTCCGCCGCTTCCATCTGCTGCGATATTCTCGATTTTGACGCTCGGATTATCGGGAAGAAAATCAATGTTCTCGCTTGGAATTTCATTCCCTTGAAACGCAGCGGCGGGTTCTTGGAGATTATCAGATGGAGCTTTCAAGATTCCAATGGCGGACTACGTCCCTGCAGTTCGAATTTTATGTATGTTCCTCTGGCTCTAGGCTCTTCGACTGGCCACGGAGATGGATCCAGAGCTCGGCATGGTATATTCGGCGCATTGGATTCGGTTAGCCCTTTGTCCAATGTTCCGTGGACAGCCGGTGAGAACAATTCGAACACCAATAAGGCTTTGGATTCGAGCCATTCCTCGAATTTCCGAGGCTTTTTACTCCAATTTATGGTCTGTGAGTGTAAATTTTGTAGTTCAAAGGATTCAGTTCTGGCCCTGAAGGATTCAGTTCGAGGGAAAGATACACATTCTTTTACCAAACCAGTGTTTGTATATTGTTCCGGTTCTGCTTCATGTTGGCATCCTGTATTTACGAAACTTGTAGGAAATGTTGTTGCTTTTTCGGGCTTGAAAAAGAAGGTGGTTTTTATAGGAAAGAAAGAGTCTAAACTAATGTATGTAACTACGGAAACTTCTGCATTGCATCTGCTTAGATTGTCCAAAAACTGGTTGCCGAAATGTGCAGCTGTTACAAAAGGAAAAGGTGAATGTGGCACGTATAGAGGGCTAGTCAAAGGGGTGTATATGCACGGGATGGTTGTCGAGCTGGACAATGAGGTGTGGCTTTTATTAACTGATCAACTGCTTACTCCACCACATAGTCTAAGGGCGGGTGCTCTCGTTACTGTGAGAAATACCCATTTTGTGAATCCCAGATTTTCTTGGACAAGAATGCTTATACTTGGTGCTTGCTTTAAGACTAGTGTTATTGTGGAATCTTTCTCTCCATTGGAAACTGGGTGTCATGTACTTTTACATTCACAGAGTTCGTTGGAGAAGTTCGTTGAGTCCTTAGCATTTTCTGCCAGATTATGGGTATTGCTTGTTGCCACATGTTTCCGGAAAAAGTTTGCTGGTATCTTATCTGAAAAGGAGATTTTGGGAACAAAATATAAGGAAGGGTTGGTTCAGACATATGCTGGTTCACATTTCCCTTCTTCGATGCATAAAACTCGGCATGGAGTATTTACAGAACTGTGCAACCATGATTCATGTGTCTGCAGTTGTGAACCATACATTGGCAACCTGAATTTGGTGGTGCCCTTATCTATTTTCATTTGCCGTTGTGAGGCCTTATGGATGAAAGCAGTGCAGTTGGAAAGTAACTGTGGAAATTTGCACGATAACAAGAAATATAGCCTTCAGTTCTGTGAAGGAACATCTCATGTTCAGTCAGTTAGAAAGATTTTTTCAAGTGAAGATTTAGGTGTAACTTTGATTGGAAGTTTGAAGACTTCTCCATCTTCTGGAAGACTGCAGTTGGTTGATGCAACTGGGAGCATTGATGTTCTTGTACCAGACCTTCCATCAACTCAGGATGCCAGTAGGATATACAAGGTAGTTGACTACAGTCTTGTTATTGAAGGAATGCCCCGGTCTGAGGATTGTACAGGATTGCTTGACAGTGATTTATTTTCATGCAGAAACATCTTTCATCTGATACCATTGACAAGAAAGATGAACTTAACAGTTTATGTTTACTTTTGCCTGAGGAATTCTATGTGCAGAAATCTTCCCTTCTATCCTTGTACTGGACTTGGAGAAGATTTGAAGAGACTTGAAAGTGAAACGTTTGACCTGCTTTTGGTTACCCACAAATTTCCAGTTCTACAGAAGTTTTCAGGTGATTTGGTGATAGCGAACCCTTCAAGTATGTTTGTGGAGGCCATAATCTTGCCTTGGAAATTATTGTTTTCTGGAAGTGATCAAATTTCATATCCAACTAAGGTTTTAGGGGATGATCCGAAGAATTCCATGGAACCTTCTCCCAAGAGATGGAAGGTTGATCACTCATCAAGCAGGGGTTCAAGTTCCAGTATAATAGGCACTTCCGGTGAGCCAACCAGAGAACTGAGTTCTTGTTCTATTTACATGGAATCTAGTGAGAAACAGAGATATTGTGACTTGAGTTCTCGTAAGATATCTTGTTCAGCTATGATTAGTGGAGTTAACAGTCACAGTCTAGGTTCAGTTATCTTGCATTGCACAAGTGCCAAATCAAGTAATGGCGGCTTTTCCAGACAAAGTGGACAGAAGGTATTGCTGGAGTTTACATCTGAAAGTTTTTACAAGTATCAGTTGTTGCAGATTGGTTGTTATTATATGGCAAAGCATGATGGAGAAGATTCCTTTTGTAATCTCAGGGATTCTAGCTTTTCGAGCAGTGATTATATTCATATCCCTTCCAAGACCCATCTGTGGAGCCTTTTATTTACTCCTGATGAGAACTGTCAAAATAGTTCATCAAAAAATCTTCCATTAGATAATTCTCTGATAAATGATGAGGTGTCTGAAGAGCGTGATGAAGTACTTCTTCAGATGTCCCCTGGAAATGTTTCAGATATATCTCTCTGTCTCCCTGAAAATGCAATCGGTTTTGGAGAGGTAATCCTGACCGAATTGGAAGAGGGCCTGATCAAACCAGTCGTGACACGTGAAGAGGTTTTAATGACTCCGCCTCTTCTGTCACTTGATTTGAACTGCCTATTTCCTCAGGGAAATCTATCATCTTTGTGTGGGCAAGTGGTTGCTGTTCATAGTGTTGAAGAGAATTCTGTCGATTCATTTTTGAACTATCAGAACATTGGTGATCCTCTGCAGCTGAGATTATTGCAGAGAGATACGAGCAGCTGTATTCACGTTTTGGTGGACCATCAGATTGTAAGGCTATCTAGTTCTTGGAGTAAACATTCCTTTCCTGTCGGGTTTGGACCTGGTGTGGATGCAACGTTTCACCGGGTTCTTGAATTGCGGGGGCAAAATAGATGGATGTTGACAGCTGCATCATTTATTGTAATCAATTCCATAAGGGCAGTATGCTTTAACAAAAATTCAGTAAGGGTAGTCAATGAATCGTGCAGTGACAAGCACTCCGGTCCTGCTTCATACGTTCAGAATGATGGGCCTCTAGACATTGTTTCTTCAGGTTTGATTTCTGAGTTGGTACAGTGCTTGGATTCAAAGCCAATGCAGTTTCATTGCAGGGTTGTTGCTATCCATATTGTGGTTTTGGAGAGGAAAATTAGAAATGCCAATTATCAGCCTACAGATCACTTCAGGCCATATCTTGTCAACATCCCGCTTGCTGGTTTTGTTTTGGACGACGGCTCATCCCCATGTTGTTGCTGGGCTAATTCCGAAAGAGCAGCAACCTTGCTAAGGCTGTATGAAGAATTCCCAGTGTCGGCTTTCGATACCAGTGGCTGGACCTTCAAGTGGATGAGAAAAAACAACAATGCATCTAGTGCCACCGTCTACCATCTTGATAGAATTTTAAAGAACCATGACCGGATTACAGTGAAGAACTTCGGATCAATGTTTGAGTCTTCCTATCAAGATCTCACTGTCTCTGTGAGTTCAGAAAACGCCCTCAGCAGCTACGACGAAAACCTCTTAAAGTTCATAATCTTTCACGCTTGCTTCAGCAAAATTTGGACGATTGTCGGGAGGATGATGGATTCGAATGCAATCGAGCGGTTGAAGACGGCGAACGTTTTGGAAACGGAGGCGTCTGTGCATTCCATGCAACATGTTTGGGCCAGAGAAGTTCACTACACAAGTCCTCTCACCGAGGCCAGGAATGTTTTACAGCAACTGCTCGACGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CTC1_2

Protein Analysis

1366

Amino Acids

151.27

Weight (kDa)

7.47

Isoelectric Point (pI)

47.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CTC1_2 PF15491 59 - 369 4e-99 CST, telomere maintenance, complex subunit CTC1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2076
AccB1I GGYRCC 1 cut(s) 1528
AccB7I CCANNNNNTGG 8 cut(s) 658, 936, 1302, 1508, 1736, 2265, 3493, 3693
AccBSI CCGCTC 1 cut(s) 3964
AccI GTMKAC 1 cut(s) 3744
AccII CGCG 4 cut(s) 41, 43, 45, 112
AccIII TCCGGA 1 cut(s) 1329
AclI AACGTT 3 cut(s) 2060, 3218, 3981
AclWI GGATC 5 cut(s) 501, 514, 2225, 3074, 3808
AcoI YGGCCR 2 cut(s) 46, 493
AcuI CTGAAG 7 cut(s) 728, 1616, 2789, 2802, 3396, 3529, 3895
AcvI CACGTG 1 cut(s) 2924
AcyI GRCGYC 1 cut(s) 3998
AfaI GTAC 6 cut(s) 1239, 1785, 1879, 2020, 2799, 3435
AflIII ACRYGT 3 cut(s) 1322, 2921, 4018
AhlI ACTAGT 1 cut(s) 1190
AjiI CACGTC 1 cut(s) 66
AjnI CCWGG 3 cut(s) 2815, 3202, 4064
AjuI GAANNNNNNNTTGG 2 cut(s) 1491, 1523
Alw21I GWGCWC 3 cut(s) 251, 517, 1108
Alw26I GTCTC 3 cut(s) 2038, 2849, 3841
AlwI GGATC 5 cut(s) 501, 514, 2225, 3074, 3808
AlwNI CAGNNNCTG 2 cut(s) 3693, 4084
Aor13HI TCCGGA 1 cut(s) 1329
ApeKI GCWGC 9 cut(s) 230, 300, 381, 953, 3088, 3117, 3269, 3635, 3864
ArsI GACNNNNNNTTYG 2 cut(s) 80, 112
Asp700I GAANNNNTTC 3 cut(s) 2799, 2824, 3794
AspLEI GCGC 4 cut(s) 43, 45, 114, 536
AspS9I GGNCC 8 cut(s) 703, 2899, 3139, 3200, 3365, 3392, 3696, 4026
AsuC2I CCSGG 2 cut(s) 1864, 3227
AsuHPI GGTGA 9 cut(s) 49, 593, 986, 2126, 2134, 2336, 3089, 3215, 4048
AsuII TTCGAA 4 cut(s) 86, 452, 593, 3950
AvaII GGWCC 4 cut(s) 3139, 3200, 3365, 3696
AxyI CCTNAGG 2 cut(s) 1979, 2982
BaeGI GKGCMC 1 cut(s) 1533
BalI TGGCCA 1 cut(s) 495
BamHI GGATCC 1 cut(s) 506
BanI GGYRCC 1 cut(s) 1528
BanII GRGCYC 1 cut(s) 517
BarI GAAGNNNNNNTAC 2 cut(s) 1776, 1808
BbrPI CACGTG 1 cut(s) 2924
BbsI GAAGAC 4 cut(s) 1727, 1747, 3807, 3977
Bbv12I GWGCWC 3 cut(s) 251, 517, 1108
BbvCI CCTCAGC 1 cut(s) 3860
BbvI GCAGC 9 cut(s) 217, 287, 393, 965, 3100, 3129, 3256, 3647, 3876
BceAI ACGGC 6 cut(s) 33, 176, 295, 1536, 3610, 3990
BcgI CGANNNNNNTGC 4 cut(s) 525, 559, 1424, 1458
BciT130I CCWGG 3 cut(s) 2817, 3204, 4066
BclI TGATCA 4 cut(s) 1066, 2194, 2275, 2904
BcnI CCSGG 2 cut(s) 1864, 3227
BcoDI GTCTC 3 cut(s) 2038, 2849, 3841
BcuI ACTAGT 1 cut(s) 1190
BfmI CTRYAG 7 cut(s) 445, 1489, 1745, 1837, 2099, 3086, 3534
BfuAI ACCTGC 1 cut(s) 2076
BglII AGATCT 1 cut(s) 3826
BmcAI AGTACT 1 cut(s) 2799
Bme1390I CCNGG 5 cut(s) 1864, 2817, 3204, 3227, 4066
Bme18I GGWCC 4 cut(s) 3139, 3200, 3365, 3696
BmgBI CACGTC 1 cut(s) 66
BmgT120I GGNCC 8 cut(s) 703, 2899, 3139, 3200, 3365, 3392, 3696, 4026
BmiI GGNNCC 6 cut(s) 205, 283, 508, 1530, 2250, 2693
BmrFI CCNGG 5 cut(s) 1864, 2817, 3204, 3227, 4066
BmrI ACTGGG 3 cut(s) 1236, 1773, 3662
BmsI GCATC 9 cut(s) 242, 799, 922, 1422, 1747, 1798, 3202, 3281, 3737
BmuI ACTGGG 3 cut(s) 1236, 1773, 3662
BoxI GACNNNNGTC 2 cut(s) 1839, 3929
BpiI GAAGAC 4 cut(s) 1727, 1747, 3807, 3977
BpmI CTGGAG 1 cut(s) 2555
Bpu10I CCTNAGC 3 cut(s) 1282, 3647, 3860
Bpu14I TTCGAA 4 cut(s) 86, 452, 593, 3950
BpuEI CTTGAG 1 cut(s) 2416
BpuMI CCSGG 2 cut(s) 1864, 3227
BsaAI YACGTR 2 cut(s) 987, 2924
BsaHI GRCGYC 1 cut(s) 3998
BsaJI CCNNGG 8 cut(s) 496, 569, 637, 1862, 2168, 2244, 2815, 4059
BsaWI WCCGGW 5 cut(s) 770, 1329, 2321, 3362, 3777
BsaXI ACNNNNNCTCC 2 cut(s) 3344, 3374
Bse118I RCCGGY 1 cut(s) 578
Bse21I CCTNAGG 2 cut(s) 1979, 2982
Bse3DI GCAATG 3 cut(s) 908, 2470, 3466
BseAI TCCGGA 1 cut(s) 1329
BseBI CCWGG 3 cut(s) 2817, 3204, 4066
BseDI CCNNGG 8 cut(s) 496, 569, 637, 1862, 2168, 2244, 2815, 4059
BseMI GCAATG 3 cut(s) 908, 2470, 3466
BseMII CTCAG 9 cut(s) 1817, 1860, 1970, 2333, 2644, 2996, 3083, 3417, 3874
BsePI GCGCGC 1 cut(s) 41
BseRI GAGGAG 1 cut(s) 165
BseSI GKGCMC 1 cut(s) 1533
BseX3I CGGCCG 1 cut(s) 46
BseXI GCAGC 9 cut(s) 217, 287, 393, 965, 3100, 3129, 3256, 3647, 3876
BseYI CCCAGC 1 cut(s) 3615
BsgI GTGCAG 4 cut(s) 972, 1601, 2014, 3367
Bsh1236I CGCG 4 cut(s) 41, 43, 45, 112
Bsh1285I CGRYCG 1 cut(s) 49
BshNI GGYRCC 1 cut(s) 1528
BsiEI CGRYCG 1 cut(s) 49
BsiHKAI GWGCWC 3 cut(s) 251, 517, 1108
BsiSI CCGG 8 cut(s) 579, 771, 1330, 1864, 2322, 3226, 3363, 3778
BslFI GGGAC 3 cut(s) 36, 426, 2797
BsmAI GTCTC 3 cut(s) 2038, 2849, 3841
BsmFI GGGAC 3 cut(s) 36, 426, 2797
BsmI GAATGC 4 cut(s) 1167, 1863, 3958, 4006
Bsp119I TTCGAA 4 cut(s) 86, 452, 593, 3950
Bsp1286I GDGCHC 4 cut(s) 251, 517, 1108, 1533
Bsp13I TCCGGA 1 cut(s) 1329
Bsp1407I TGTACA 1 cut(s) 1877
Bsp19I CCATGG 1 cut(s) 2244
BspCNI CTCAG 9 cut(s) 1816, 1861, 1971, 2334, 2643, 2995, 3084, 3418, 3873
BspEI TCCGGA 1 cut(s) 1329
BspFNI CGCG 4 cut(s) 41, 43, 45, 112
BspLI GGNNCC 6 cut(s) 205, 283, 508, 1530, 2250, 2693
BspMAI CTGCAG 4 cut(s) 449, 1493, 1749, 3090
BspMI ACCTGC 1 cut(s) 2076
BspPI GGATC 5 cut(s) 501, 514, 2225, 3074, 3808
BspQI GCTCTTC 2 cut(s) 490, 2778
BspT104I TTCGAA 4 cut(s) 86, 452, 593, 3950
BspT107I GGYRCC 1 cut(s) 1528
BsrBI CCGCTC 1 cut(s) 3964
BsrDI GCAATG 3 cut(s) 908, 2470, 3466
BsrFI RCCGGY 1 cut(s) 578
BsrGI TGTACA 1 cut(s) 1877
BssAI RCCGGY 1 cut(s) 578
BssECI CCNNGG 8 cut(s) 496, 569, 637, 1862, 2168, 2244, 2815, 4059
BssHII GCGCGC 1 cut(s) 41
BssNI GRCGYC 1 cut(s) 3998
BssT1I CCWWGG 2 cut(s) 2168, 2244
Bst2UI CCWGG 3 cut(s) 2817, 3204, 4066
Bst6I CTCTTC 7 cut(s) 490, 2036, 2778, 2889, 2922, 2955, 3030
BstACI GRCGYC 1 cut(s) 3998
BstAPI GCANNNNNTGC 3 cut(s) 1292, 3734, 4012
BstAUI TGTACA 1 cut(s) 1877
BstBAI YACGTR 2 cut(s) 987, 2924
BstBI TTCGAA 4 cut(s) 86, 452, 593, 3950
BstC8I GCNNGC 4 cut(s) 43, 1180, 3573, 3907
BstDSI CCRYGG 3 cut(s) 496, 569, 2244
BstEII GGTNACC 1 cut(s) 2077
BstENI CCTNNNNNAGG 1 cut(s) 3646
BstFNI CGCG 4 cut(s) 41, 43, 45, 112
BstHHI GCGC 4 cut(s) 43, 45, 114, 536
BstMAI GTCTC 3 cut(s) 2038, 2849, 3841
BstMCI CGRYCG 1 cut(s) 49
BstMWI GCNNNNNNNGC 6 cut(s) 40, 109, 1292, 2649, 3734, 4012
BstNI CCWGG 3 cut(s) 2817, 3204, 4066
BstNSI RCATGY 2 cut(s) 1326, 4022
BstPAI GACNNNNGTC 2 cut(s) 1839, 3929
BstPI GGTNACC 1 cut(s) 2077
BstSCI CCNGG 5 cut(s) 1862, 2815, 3202, 3225, 4064
BstSFI CTRYAG 7 cut(s) 445, 1489, 1745, 1837, 2099, 3086, 3534
BstSLI GKGCMC 1 cut(s) 1533
BstUI CGCG 4 cut(s) 41, 43, 45, 112
BstV1I GCAGC 9 cut(s) 217, 287, 393, 965, 3100, 3129, 3256, 3647, 3876
BstV2I GAAGAC 4 cut(s) 1727, 1747, 3807, 3977
BstX2I RGATCY 2 cut(s) 506, 3826
BstXI CCANNNNNNTGG 1 cut(s) 503
BstYI RGATCY 2 cut(s) 506, 3826
BstZI CGGCCG 1 cut(s) 46
Bsu36I CCTNAGG 2 cut(s) 1979, 2982
BtgI CCRYGG 3 cut(s) 496, 569, 2244
BtrI CACGTC 1 cut(s) 66
BtsI GCAGTG 3 cut(s) 1584, 2659, 3355
BveI ACCTGC 1 cut(s) 2076
Cac8I GCNNGC 4 cut(s) 43, 1180, 3573, 3907
CaiI CAGNNNCTG 2 cut(s) 3693, 4084
CfoI GCGC 4 cut(s) 43, 45, 114, 536
Cfr10I RCCGGY 1 cut(s) 578
Cfr13I GGNCC 8 cut(s) 703, 2899, 3139, 3200, 3365, 3392, 3696, 4026
CseI GACGC 2 cut(s) 329, 3987
CsiI ACCWGGT 1 cut(s) 3202
Csp6I GTAC 6 cut(s) 1238, 1784, 1878, 2019, 2798, 3434
CviQI GTAC 6 cut(s) 1238, 1784, 1878, 2019, 2798, 3434
DraI TTTAAA 1 cut(s) 3765
EaeI YGGCCR 2 cut(s) 46, 493
EagI CGGCCG 1 cut(s) 46
Eam1104I CTCTTC 7 cut(s) 490, 2036, 2778, 2889, 2922, 2955, 3030
EarI CTCTTC 7 cut(s) 490, 2036, 2778, 2889, 2922, 2955, 3030
EciI GGCGGA 3 cut(s) 274, 447, 2934
Ecl136II GAGCTC 1 cut(s) 515
EclXI CGGCCG 1 cut(s) 46
Eco130I CCWWGG 2 cut(s) 2168, 2244
Eco147I AGGCCT 1 cut(s) 1562
Eco24I GRGCYC 1 cut(s) 517
Eco32I GATATC 1 cut(s) 2412
Eco47I GGWCC 4 cut(s) 3139, 3200, 3365, 3696
Eco52I CGGCCG 1 cut(s) 46
Eco53kI GAGCTC 1 cut(s) 515
Eco57I CTGAAG 7 cut(s) 728, 1616, 2789, 2802, 3396, 3529, 3895
Eco72I CACGTG 1 cut(s) 2924
Eco81I CCTNAGG 2 cut(s) 1979, 2982
Eco91I GGTNACC 1 cut(s) 2077
EcoICRI GAGCTC 1 cut(s) 515
EcoNI CCTNNNNNAGG 1 cut(s) 3646
EcoO109I RGGNCCY 1 cut(s) 2899
EcoO65I GGTNACC 1 cut(s) 2077
EcoRI GAATTC 5 cut(s) 15, 1984, 2239, 3040, 3662
EcoRII CCWGG 3 cut(s) 2815, 3202, 4064
EcoRV GATATC 1 cut(s) 2412
EcoT14I CCWWGG 2 cut(s) 2168, 2244
EcoT22I ATGCAT 2 cut(s) 1437, 3730
EcoT38I GRGCYC 1 cut(s) 517
ErhI CCWWGG 2 cut(s) 2168, 2244
FalI AAGNNNNNCTT 4 cut(s) 1937, 1969, 2787, 2819
FaqI GGGAC 3 cut(s) 36, 426, 2797
FauI CCCGC 4 cut(s) 380, 1093, 3234, 3576
FauNDI CATATG 1 cut(s) 1405
FbaI TGATCA 4 cut(s) 1066, 2194, 2275, 2904
FblI GTMKAC 1 cut(s) 3744
FriOI GRGCYC 1 cut(s) 517
GlaI GCGC 4 cut(s) 42, 44, 113, 535
GsaI CCCAGC 1 cut(s) 3619
GsuI CTGGAG 1 cut(s) 2555
HapII CCGG 8 cut(s) 579, 771, 1330, 1864, 2322, 3226, 3363, 3778
HgaI GACGC 2 cut(s) 329, 3987
HhaI GCGC 4 cut(s) 43, 45, 114, 536
Hin1I GRCGYC 1 cut(s) 3998
Hin6I GCGC 4 cut(s) 41, 43, 112, 534
HinP1I GCGC 4 cut(s) 41, 43, 112, 534
HincII GTYRAC 4 cut(s) 1834, 2440, 3264, 3562
HindII GTYRAC 4 cut(s) 1834, 2440, 3264, 3562
HpaI GTTAAC 1 cut(s) 2440
HpaII CCGG 8 cut(s) 579, 771, 1330, 1864, 2322, 3226, 3363, 3778
HphI GGTGA 9 cut(s) 49, 593, 986, 2126, 2134, 2336, 3089, 3215, 4048
Hpy99I CGWCG 5 cut(s) 203, 281, 3596, 3875, 4094
HpyCH4IV ACGT 9 cut(s) 65, 439, 986, 2060, 2923, 3129, 3218, 3378, 3981
HpyF10VI GCNNNNNNNGC 6 cut(s) 40, 109, 1292, 2649, 3734, 4012
HpySE526I ACGT 9 cut(s) 65, 439, 986, 2060, 2923, 3129, 3218, 3378, 3981
Hsp92I GRCGYC 1 cut(s) 3998
HspAI GCGC 4 cut(s) 41, 43, 112, 534
Kpn2I TCCGGA 1 cut(s) 1329
Ksp22I TGATCA 4 cut(s) 1066, 2194, 2275, 2904
KspAI GTTAAC 1 cut(s) 2440
LguI GCTCTTC 2 cut(s) 490, 2778
LmnI GCTCC 7 cut(s) 108, 119, 254, 287, 411, 1767, 2691
Lsp1109I GCAGC 9 cut(s) 217, 287, 393, 965, 3100, 3129, 3256, 3647, 3876
LweI GCATC 9 cut(s) 242, 799, 922, 1422, 1747, 1798, 3202, 3281, 3737
MabI ACCWGGT 1 cut(s) 3202
MaeII ACGT 9 cut(s) 65, 439, 986, 2060, 2923, 3129, 3218, 3378, 3981
MauBI CGCGCGCG 1 cut(s) 41
MbiI CCGCTC 1 cut(s) 3964
MflI RGATCY 2 cut(s) 506, 3826
MhlI GDGCHC 4 cut(s) 251, 517, 1108, 1533
MlsI TGGCCA 1 cut(s) 495
MluNI TGGCCA 1 cut(s) 495
MlyI GAGTC 4 cut(s) 881, 1286, 2935, 3821
MmeI TCCRAC 5 cut(s) 181, 300, 1242, 1566, 2234
Mox20I TGGCCA 1 cut(s) 495
Mph1103I ATGCAT 2 cut(s) 1437, 3730
MroI TCCGGA 1 cut(s) 1329
MroXI GAANNNNTTC 3 cut(s) 2799, 2824, 3794
MscI TGGCCA 1 cut(s) 495
MseI TTAA 8 cut(s) 1061, 1185, 1955, 2439, 2936, 3312, 3764, 3884
MslI CAYNNNNRTG 3 cut(s) 501, 3001, 3604
Msp20I TGGCCA 1 cut(s) 495
MspA1I CMGCKG 6 cut(s) 230, 384, 956, 3091, 3120, 3269
MspI CCGG 8 cut(s) 579, 771, 1330, 1864, 2322, 3226, 3363, 3778
MspR9I CCNGG 5 cut(s) 1864, 2817, 3204, 3227, 4066
Mva1269I GAATGC 4 cut(s) 1167, 1863, 3958, 4006
MvaI CCWGG 3 cut(s) 2817, 3204, 4066
MvnI CGCG 4 cut(s) 41, 43, 45, 112
MwoI GCNNNNNNNGC 6 cut(s) 40, 109, 1292, 2649, 3734, 4012
NciI CCSGG 2 cut(s) 1864, 3227
NcoI CCATGG 1 cut(s) 2244
NdeI CATATG 1 cut(s) 1405
NlaIV GGNNCC 6 cut(s) 205, 283, 508, 1530, 2250, 2693
NmeAIII GCCGAG 2 cut(s) 496, 1423
NmuCI GTSAC 5 cut(s) 2390, 2444, 2917, 2955, 3350
NsiI ATGCAT 2 cut(s) 1437, 3730
NspI RCATGY 2 cut(s) 1326, 4022
NspV TTCGAA 4 cut(s) 86, 452, 593, 3950
PauI GCGCGC 1 cut(s) 41
PceI AGGCCT 1 cut(s) 1562
PciI ACATGT 2 cut(s) 1322, 4018
PciSI GCTCTTC 2 cut(s) 490, 2778
PcsI WCGNNNNNNNCGW 1 cut(s) 195
PctI GAATGC 4 cut(s) 1167, 1863, 3958, 4006
PdmI GAANNNNTTC 3 cut(s) 2799, 2824, 3794
PflFI GACNNNGTC 1 cut(s) 438
PflMI CCANNNNNTGG 8 cut(s) 658, 936, 1302, 1508, 1736, 2265, 3493, 3693
PleI GAGTC 4 cut(s) 880, 1285, 2935, 3820
PmaCI CACGTG 1 cut(s) 2924
PmlI CACGTG 1 cut(s) 2924
PpsI GAGTC 4 cut(s) 880, 1285, 2935, 3820
Ppu21I YACGTR 2 cut(s) 987, 2924
PscI ACATGT 2 cut(s) 1322, 4018
PshAI GACNNNNGTC 2 cut(s) 1839, 3929
Psp124BI GAGCTC 1 cut(s) 517
Psp1406I AACGTT 3 cut(s) 2060, 3218, 3981
Psp6I CCWGG 3 cut(s) 2815, 3202, 4064
PspCI CACGTG 1 cut(s) 2924
PspEI GGTNACC 1 cut(s) 2077
PspFI CCCAGC 1 cut(s) 3615
PspGI CCWGG 3 cut(s) 2815, 3202, 4064
PspN4I GGNNCC 6 cut(s) 205, 283, 508, 1530, 2250, 2693
PspPI GGNCC 8 cut(s) 703, 2899, 3139, 3200, 3365, 3392, 3696, 4026
PstI CTGCAG 4 cut(s) 449, 1493, 1749, 3090
PstNI CAGNNNCTG 2 cut(s) 3693, 4084
PsuI RGATCY 2 cut(s) 506, 3826
PsyI GACNNNGTC 1 cut(s) 438
PteI GCGCGC 1 cut(s) 41
PvuII CAGCTG 4 cut(s) 956, 3091, 3120, 3269
RsaI GTAC 6 cut(s) 1239, 1785, 1879, 2020, 2799, 3435
RsaNI GTAC 6 cut(s) 1238, 1784, 1878, 2019, 2798, 3434
RseI CAYNNNNRTG 3 cut(s) 501, 3001, 3604
SacI GAGCTC 1 cut(s) 517
SapI GCTCTTC 2 cut(s) 490, 2778
SaqAI TTAA 8 cut(s) 1061, 1185, 1955, 2439, 2936, 3312, 3764, 3884
Sau96I GGNCC 8 cut(s) 703, 2899, 3139, 3200, 3365, 3392, 3696, 4026
ScaI AGTACT 1 cut(s) 2799
SchI GAGTC 4 cut(s) 881, 1286, 2935, 3821
ScrFI CCNGG 5 cut(s) 1864, 2817, 3204, 3227, 4066
SduI GDGCHC 4 cut(s) 251, 517, 1108, 1533
SexAI ACCWGGT 1 cut(s) 3202
SfaNI GCATC 9 cut(s) 242, 799, 922, 1422, 1747, 1798, 3202, 3281, 3737
SfcI CTRYAG 7 cut(s) 445, 1489, 1745, 1837, 2099, 3086, 3534
SfuI TTCGAA 4 cut(s) 86, 452, 593, 3950
SinI GGWCC 4 cut(s) 3139, 3200, 3365, 3696
SmiMI CAYNNNNRTG 3 cut(s) 501, 3001, 3604
SmlI CTYRAG 1 cut(s) 2395
SmoI CTYRAG 1 cut(s) 2395
SpeI ACTAGT 1 cut(s) 1190
SseBI AGGCCT 1 cut(s) 1562
SstI GAGCTC 1 cut(s) 517
StuI AGGCCT 1 cut(s) 1562
StyD4I CCNGG 5 cut(s) 1862, 2815, 3202, 3225, 4064
StyI CCWWGG 2 cut(s) 2168, 2244
TaiI ACGT 9 cut(s) 68, 442, 989, 2063, 2926, 3132, 3221, 3381, 3984
TaqII GACCGA 1 cut(s) 2900
TatI WGTACW 4 cut(s) 1237, 1877, 2018, 2797
TauI GCSGC 4 cut(s) 48, 290, 387, 2502
Tru1I TTAA 8 cut(s) 1061, 1185, 1955, 2439, 2936, 3312, 3764, 3884
Tru9I TTAA 8 cut(s) 1061, 1185, 1955, 2439, 2936, 3312, 3764, 3884
TseFI GTSAC 5 cut(s) 2390, 2444, 2917, 2955, 3350
TseI GCWGC 9 cut(s) 230, 300, 381, 953, 3088, 3117, 3269, 3635, 3864
Tsp45I GTSAC 5 cut(s) 2390, 2444, 2917, 2955, 3350
TspGWI ACGGA 5 cut(s) 106, 513, 558, 911, 4007
Tth111I GACNNNGTC 1 cut(s) 438
Van91I CCANNNNNTGG 8 cut(s) 658, 936, 1302, 1508, 1736, 2265, 3493, 3693
VpaK11BI GGWCC 4 cut(s) 3139, 3200, 3365, 3696
XagI CCTNNNNNAGG 1 cut(s) 3646
XbaI TCTAGA 1 cut(s) 3397
XceI RCATGY 2 cut(s) 1326, 4022
XcmI CCANNNNNNNNNTGG 2 cut(s) 607, 3612
XmiI GTMKAC 1 cut(s) 3744
XmnI GAANNNNTTC 3 cut(s) 2799, 2824, 3794
ZrmI AGTACT 1 cut(s) 2799
Zsp2I ATGCAT 2 cut(s) 1437, 3730
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.