Rw7G031300

CST complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0006351
Physical Location & Seq
Forward (+)
44749585 .. 44756065
6481 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G031300.1

Sequence Viewer

Length: 3669 bp
ATGGAGTTAGGCTCTTTCACTGCTAATGCTAATGAAGACAGTTCCAAGGCTCGTCATGGTGTGTTCGGCGCACTGGAGTCGGTTAGCCCTTTCTCCATTGTTTCATGTAGAACAGGTGATAGCAATTCGAAAAGCAACAAGGCTTTGGATTCAGGCCCTGCGACAAGCCTCCGCGGCTTCTTAGTCCAATTTATGGTTTGTGAGTGTAGATCATGCAGTTCAAAGGAGTCAATCATGGTTTTGAAGGATTCAATTCAAGAAAGAGATACTCATTCTTTTACCAAACCAATGTTTGTGTATTGTTGCGGTTCTGCTTCATCCTGGCATCCGGTATTTACGAAACTTGTGGGAAATATTGTTGCTATATCAGGCTTGAAAAAGAAGTTGGTTTTTATAGGGAAGGAAGAGTCCAAAGTAATGTGTGTAACTACAGGAAGTTCTGCCTTGCATTTGAGCAGATTGTCCAAGAAATGGACGCCGAAAGTTACAGTAGGTAGAAAGGGAAATGGGGAAGTTGGTACATATAGAGGGATAGTTAAAGGTGTGTATATGCAAGGAATGGTTGTGGAATTGGACAATGAGGTGTGGCTTTTGTTAACTGATCAACTGCTTACTCCACCACATAGTCTTAGGGTGGGTGCTCTTGTTTCTGTGAGAAATGCCCATTTTGTAAATCCCAGATTTTCTTGGACAAGAAGAATGCTTATACTTGGTGGTTGCTTTAGGACCAATGTAATTGTGGAATCCTTCTCTCCAATAGAAACTCGGTGTCATATAGCCTCACAATCACAGAGTTCCTTGGGGAAGTTCATCGAGTCCTTAGCATTTTCTTCCAGATTATGGGTATTGCTGGTTGCCTCGTATTTCCGGAAAAAGTTTGCTGGTATCTTATCTGAGAAGGAAATTTTGGGCTCAAAACATAAGGGAGGATTAGTTGAGATGTTTGCTAGTTCACAGATGCCTTCATCAATGCATCAAACTCGGCCGGGAGTATTCATGGAATTTTGCAAGCATGATTCCTGTGCCTGTGGTTGTGAGCCATACATAGATAATCTGACTCTGGTGATCCCCTTGTCTTTTTTCATATGTCACTGTGAGCCCACATGGATGAGGGCACTGAACCTGGAAGGTAACAGTAGACAATTTCATGATGACAAACAGTACAGCCGTCAATTATGTGAAGGGAGATCTTATGTTCAGTCTATTAGAAAGATCTTTTCAAGTCATGATATAGGCATAACTTTGATTGGAAGTTTGAAGACTTCTCCATCTTCCGGAAGACTGCAGTTGGTTGATGCAACTGGTAGCATTGATGTTCTTGTACCAGACCTTCCATCAACTTGGGATGCCACTAGAATAATCAAGGTAGTTGACTACAGTGTAATTATTGAAGGCATGCCTGGGTCTGTGGATTCTGGAGGATTGCTTGAATATGATTTGTTCTCAACTCGAGCTATCTTTGATTTTGTACCATTGGCAAGAAAGGTGAACTTAACAGTTTGTGTTTACTTTCGCTTGAGAAGTCCGTTGTGCAGAAATCTTTGCTTTTATCCTTGTACTGGATTGGGAGAAGATTTGAAGAGATTTGAAGGTGGAACATTTCACCTGCTATGGATTACCCACAAATTTCCTGTGTTACACAAGCTTCAAGGTGATGTGTTAACAAGCTCATCAAGTATGTTTGTTGAGGCTATAATCTTGCCATGGAATTTATCAGTTGCTGAAAACAATGGTATTGCATGTCAAACTGGGGTAGTTGGGGATGATCCAAAAAATTCCATGGAATTTTGTGCTGTTGGATGTTACTTGAAGAATGATTCTTTCAAGAGACGGAAGGTTTGTGATTCATCAAGGAAGGAACTAAGTTCTGGTTCAATGGACTGTTCCTTTGAGGCCGTCGGAAAAGTGAATTCTTGTTCTGAGTCTTACATTGAATCCAGTGAGGATAAAACATATAGCAACTTGAGTTGTCCTGAAATATCTGGTTTGGCTATAATTAGTGGAGTTACTCGTTCAGTTATCTTGTACCGTACCAAAGCCAAATTAAACAGTGGTGGCTTTTGCGGTCCAAGTGGACAGAAGATATTGCTCGAGTTTAAGTCTGACAGCTTTTATAAGTATCAGCTGTTGCAGATTGGCTGTTATTATATCACAAAGCATGATAGAGAAAATTCTTTTTGTAAACTTAAGGGCTCTGACTATTTCATTGGTAAAAAAATTCTCATCCCTCCCACAACTCATCTGTGGAGCCTTTCATTTGGTTCTGATAAGGTTTGTCAAAATAACAGTTCATCAAAGTGTATTCCATTGGATGATTCTCTTAGAAGTGACGAGCTTCTATCTGGATATCATAACGAAGTACTTCTTCAGACGTCCAATGAAAATTTGTCTGAAACCTCTTCAGACATGTGTCTCTGTCTGTCTGCAAGCGTATTAGGTCTTGGAGAGCTGCACCTAAAGGAACTGAAAGAAAGCCTGATCAAGCCAGTTGTGACACCTAAAGACATTCCAAAAATCTCTTCATGCATTAGGCCTGTAACAACTGCGCCTCCACTATCAACTGAATCTAACCGTATGTTTCCTGAGGGAGACCTAATATCTATGCGTGGGCATGTGGTTGCGGTTCATAGCATTGAGGACAACTCTGTTGATCCATATTTGAACCGTCAAAACCTTCGTGATCCTCTTGAGCTGAGATTCTTGCAGAGGACAACAAGCAGTTGCATTCACGTGTTGGTGGATAATCAAATTGTAAAGCTATCTGGTTCTTTGTGCAAACATGATTTTCCAGTCGGATTTGGACCTGGTGTGGATGCAACGTTTTATCGGATTCTTGCACTGTGGGAGCAGAATAGATGGATCTTGACATCGGTATCATTTATTACCATCCATTCCATAAGTGGAGACAATGAATCATGTGGTGTCAAGTGCTCTAATCCTGCTTCATACATGCCCAGTGCTTCGCCTCAAGAGATCGCCCATTCAGGGTTGATATCTGAATTGGTTCAAAGTTTGGACTTCAAGCCAGTGCTGTTGCATTGTAGGGTTGTTGCTATCCATTTCCTGGTTCTAGAGAAGAAAAGTAGAAATGTCAATTATCAACTAAAAAATCACTTGAGGCAGCATCTTGTTGATGTCCCACTTGCTGGCTTTGTTCTAGATGATGGTTCTTCTCCATGCTGTTGTTGGGCTAATGCTGAAAGAGCAGCAACCTTGCTGAGGCTGCATGAAGAATTCCCACAGTTAGCTTTTGAAAATAGTGGCTGGACCTTAAAGTGGGTCAGAACAAACAATAATTCCTGGAGTTCCACTATCTGTCATCTCGAGAGAATTTTAAATAATCATCATCGTATTGTAGTGAGGAACTATGGATCAATGTTTGATTCGTCTTATCAAGATCTCGCTGTGTCTGTGAGTTCAGATAATGCCCTAAGCAGCTATGATGAAAACCTTCTAAAGTTTATAGTCTTCAATGCTTGCTTCAGCAAATTTTGGACCATTGGTGCTAGTAGGATGGATTTGGATGCTGTCAGGCTATTGAAGACAGAGAACCTTGTGGAAATGGAGATGTCTATGCAATCCATGCAACATATATGGGCCACAGAAGTTCAACACATTAACCATCTCAGTCAGGCCAGGAATGTTATTCAAGAACTGATAGACGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CTC1_2

Protein Analysis

1222

Amino Acids

135.99

Weight (kDa)

8.04

Isoelectric Point (pI)

46.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CTC1_2 PF15491 9 - 214 8e-66 CST, telomere maintenance, complex subunit CTC1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2115
AarI CACCTGC 1 cut(s) 1614
AatII GACGTC 1 cut(s) 2375
Acc36I ACCTGC 1 cut(s) 1614
AccB7I CCANNNNNTGG 5 cut(s) 193, 471, 840, 3064, 3146
AccI GTMKAC 1 cut(s) 1138
AccII CGCG 1 cut(s) 174
AccIII TCCGGA 2 cut(s) 867, 1274
AciI CCGC 5 cut(s) 172, 174, 306, 2064, 2621
AclI AACGTT 1 cut(s) 2819
AclWI GGATC 6 cut(s) 1060, 1760, 2645, 2675, 2867, 3379
AcoI YGGCCR 1 cut(s) 983
AcuI CTGAAG 3 cut(s) 2351, 2385, 3466
AcvI CACGTG 1 cut(s) 2731
AcyI GRCGYC 2 cut(s) 476, 2372
AfaI GTAC 8 cut(s) 520, 1163, 1323, 1470, 1558, 2027, 2032, 2361
AflII CTTAAG 1 cut(s) 2186
AflIII ACRYGT 2 cut(s) 2406, 2730
AjnI CCWGG 7 cut(s) 320, 1122, 1399, 2803, 3063, 3299, 3635
AjuI GAANNNNNNNTTGG 6 cut(s) 368, 400, 890, 922, 2503, 2535
Alw21I GWGCWC 2 cut(s) 643, 2933
Alw26I GTCTC 4 cut(s) 1822, 2417, 2583, 2898
AlwI GGATC 6 cut(s) 1060, 1760, 2645, 2675, 2867, 3379
AlwNI CAGNNNCTG 2 cut(s) 158, 1721
Ama87I CYCGRG 3 cut(s) 1449, 2090, 3323
Aor13HI TCCGGA 2 cut(s) 867, 1274
AoxI GGCC 6 cut(s) 154, 983, 1893, 2531, 3597, 3633
ApeKI GCWGC 5 cut(s) 2449, 3121, 3206, 3223, 3435
ArsI GACNNNNNNTTYG 4 cut(s) 1821, 1853, 1871, 1903
Asp700I GAANNNNTTC 3 cut(s) 2361, 3003, 3450
AspLEI GCGC 2 cut(s) 71, 2548
AspS9I GGNCC 7 cut(s) 155, 726, 2066, 2801, 3267, 3495, 3597
AsuC2I CCSGG 1 cut(s) 987
AsuHPI GGTGA 5 cut(s) 128, 1075, 1498, 1595, 1664
AsuII TTCGAA 1 cut(s) 128
AvaI CYCGRG 3 cut(s) 1449, 2090, 3323
AvaII GGWCC 5 cut(s) 726, 2066, 2801, 3267, 3495
AxyI CCTNAGG 1 cut(s) 2583
BaeGI GKGCMC 1 cut(s) 1117
BaeI ACNNNNGTAYC 2 cut(s) 2856, 2889
BanII GRGCYC 3 cut(s) 914, 1101, 2195
BarI GAAGNNNNNNTAC 2 cut(s) 1314, 1346
BbrPI CACGTG 1 cut(s) 2731
BbsI GAAGAC 5 cut(s) 42, 1265, 1285, 3460, 3548
Bbv12I GWGCWC 2 cut(s) 643, 2933
BbvCI CCTCAGC 1 cut(s) 3218
BbvI GCAGC 5 cut(s) 2436, 3133, 3210, 3218, 3447
BccI CCATC 7 cut(s) 1276, 1342, 2850, 2894, 3158, 3508, 3630
BceAI ACGGC 2 cut(s) 1152, 1880
BcgI CGANNNNNNTGC 4 cut(s) 60, 94, 962, 996
BciT130I CCWGG 7 cut(s) 322, 1124, 1401, 2805, 3065, 3301, 3637
BclI TGATCA 2 cut(s) 601, 2478
BcnI CCSGG 1 cut(s) 987
BcoDI GTCTC 4 cut(s) 1822, 2417, 2583, 2898
BfaI CTAG 5 cut(s) 948, 1353, 3071, 3158, 3507
BfmI CTRYAG 3 cut(s) 429, 1283, 1375
BfrI CTTAAG 1 cut(s) 2186
BfuAI ACCTGC 1 cut(s) 1614
BglI GCCNNNNNGGC 1 cut(s) 174
BglII AGATCT 3 cut(s) 1187, 1212, 3397
BisI GCNGC 6 cut(s) 175, 2450, 3122, 3207, 3224, 3436
BlsI GCNGC 6 cut(s) 176, 2451, 3123, 3208, 3225, 3437
BmcAI AGTACT 1 cut(s) 2361
Bme1390I CCNGG 8 cut(s) 322, 987, 1124, 1401, 2805, 3065, 3301, 3637
Bme18I GGWCC 5 cut(s) 726, 2066, 2801, 3267, 3495
BmeT110I CYCGRG 3 cut(s) 1449, 2090, 3323
BmgT120I GGNCC 7 cut(s) 155, 726, 2066, 2801, 3267, 3495, 3597
BmiI GGNNCC 1 cut(s) 2249
BmrFI CCNGG 8 cut(s) 322, 987, 1124, 1401, 2805, 3065, 3301, 3637
BmrI ACTGGG 2 cut(s) 1758, 2949
BmsI GCATC 8 cut(s) 334, 948, 982, 1285, 1336, 2803, 3133, 3514
BmuI ACTGGG 2 cut(s) 1758, 2949
BoxI GACNNNNGTC 1 cut(s) 2409
BpiI GAAGAC 5 cut(s) 42, 1265, 1285, 3460, 3548
BplI GAGNNNNNCTC 3 cut(s) 28, 2627, 2659
BpmI CTGGAG 3 cut(s) 95, 1437, 3322
Bpu10I CCTNAGC 3 cut(s) 820, 3218, 3431
Bpu14I TTCGAA 1 cut(s) 128
BpuEI CTTGAG 5 cut(s) 1537, 1984, 2708, 2952, 3136
BpuMI CCSGG 1 cut(s) 987
BsaAI YACGTR 1 cut(s) 2731
BsaHI GRCGYC 2 cut(s) 476, 2372
BsaI GGTCTC 1 cut(s) 2583
BsaJI CCNNGG 6 cut(s) 45, 172, 798, 1400, 1703, 1779
BsaWI WCCGGW 3 cut(s) 328, 867, 1274
BsaXI ACNNNNNCTCC 4 cut(s) 918, 948, 2533, 2563
Bse1I ACTGG 9 cut(s) 78, 1306, 1564, 1753, 1938, 2486, 2789, 2955, 3026
Bse21I CCTNAGG 1 cut(s) 2583
BseAI TCCGGA 2 cut(s) 867, 1274
BseBI CCWGG 7 cut(s) 322, 1124, 1401, 2805, 3065, 3301, 3637
BseDI CCNNGG 6 cut(s) 45, 172, 798, 1400, 1703, 1779
BseMII CTCAG 6 cut(s) 885, 1911, 2574, 2684, 3209, 3640
BseNI ACTGG 9 cut(s) 78, 1306, 1564, 1753, 1938, 2486, 2789, 2955, 3026
BseSI GKGCMC 1 cut(s) 1117
BseX3I CGGCCG 1 cut(s) 983
BseXI GCAGC 5 cut(s) 2436, 3133, 3210, 3218, 3447
BsgI GTGCAG 2 cut(s) 1552, 2435
Bsh1236I CGCG 1 cut(s) 174
Bsh1285I CGRYCG 1 cut(s) 986
BshFI GGCC 6 cut(s) 156, 985, 1895, 2533, 3599, 3635
BsiEI CGRYCG 1 cut(s) 986
BsiHKAI GWGCWC 2 cut(s) 643, 2933
BsiHKCI CYCGRG 3 cut(s) 1449, 2090, 3323
BsiSI CCGG 4 cut(s) 329, 868, 986, 1275
BslFI GGGAC 1 cut(s) 3122
BsmAI GTCTC 4 cut(s) 1822, 2417, 2583, 2898
BsmBI CGTCTC 1 cut(s) 1822
BsmFI GGGAC 1 cut(s) 3122
BsmI GAATGC 2 cut(s) 705, 2724
BsnI GGCC 6 cut(s) 156, 985, 1895, 2533, 3599, 3635
Bso31I GGTCTC 1 cut(s) 2583
BsoBI CYCGRG 3 cut(s) 1449, 2090, 3323
Bsp119I TTCGAA 1 cut(s) 128
Bsp1286I GDGCHC 6 cut(s) 643, 914, 1101, 1117, 2195, 2933
Bsp13I TCCGGA 2 cut(s) 867, 1274
Bsp19I CCATGG 2 cut(s) 1703, 1779
BspACI CCGC 5 cut(s) 172, 174, 306, 2064, 2621
BspANI GGCC 6 cut(s) 156, 985, 1895, 2533, 3599, 3635
BspCNI CTCAG 6 cut(s) 886, 1912, 2575, 2685, 3210, 3639
BspEI TCCGGA 2 cut(s) 867, 1274
BspFNI CGCG 1 cut(s) 174
BspHI TCATGA 2 cut(s) 1147, 1225
BspLI GGNNCC 1 cut(s) 2249
BspMAI CTGCAG 1 cut(s) 1287
BspMI ACCTGC 1 cut(s) 1614
BspPI GGATC 6 cut(s) 1060, 1760, 2645, 2675, 2867, 3379
BspT104I TTCGAA 1 cut(s) 128
BspTI CTTAAG 1 cut(s) 2186
BspTNI GGTCTC 1 cut(s) 2583
BsrI ACTGG 9 cut(s) 78, 1306, 1564, 1753, 1938, 2486, 2789, 2955, 3026
BssECI CCNNGG 6 cut(s) 45, 172, 798, 1400, 1703, 1779
BssNI GRCGYC 2 cut(s) 476, 2372
BssT1I CCWWGG 4 cut(s) 45, 798, 1703, 1779
Bst2UI CCWGG 7 cut(s) 322, 1124, 1401, 2805, 3065, 3301, 3637
Bst6I CTCTTC 4 cut(s) 399, 1574, 2404, 2524
BstACI GRCGYC 2 cut(s) 476, 2372
BstAFI CTTAAG 1 cut(s) 2186
BstAPI GCANNNNNTGC 1 cut(s) 3583
BstBAI YACGTR 1 cut(s) 2731
BstBI TTCGAA 1 cut(s) 128
BstC8I GCNNGC 5 cut(s) 1010, 1397, 2428, 3148, 3478
BstDSI CCRYGG 3 cut(s) 172, 1703, 1779
BstENI CCTNNNNNAGG 1 cut(s) 3217
BstFNI CGCG 1 cut(s) 174
BstHHI GCGC 2 cut(s) 71, 2548
BstMAI GTCTC 4 cut(s) 1822, 2417, 2583, 2898
BstMCI CGRYCG 1 cut(s) 986
BstMWI GCNNNNNNNGC 4 cut(s) 174, 3203, 3223, 3583
BstNI CCWGG 7 cut(s) 322, 1124, 1401, 2805, 3065, 3301, 3637
BstNSI RCATGY 5 cut(s) 1399, 1743, 2410, 2615, 2953
BstPAI GACNNNNGTC 1 cut(s) 2409
BstSCI CCNGG 8 cut(s) 320, 985, 1122, 1399, 2803, 3063, 3299, 3635
BstSFI CTRYAG 3 cut(s) 429, 1283, 1375
BstSLI GKGCMC 1 cut(s) 1117
BstUI CGCG 1 cut(s) 174
BstV1I GCAGC 5 cut(s) 2436, 3133, 3210, 3218, 3447
BstV2I GAAGAC 5 cut(s) 42, 1265, 1285, 3460, 3548
BstX2I RGATCY 4 cut(s) 1187, 1212, 2859, 3397
BstXI CCANNNNNNTGG 1 cut(s) 1341
BstYI RGATCY 4 cut(s) 1187, 1212, 2859, 3397
BstZI CGGCCG 1 cut(s) 983
Bsu36I CCTNAGG 1 cut(s) 2583
BsuRI GGCC 6 cut(s) 156, 985, 1895, 2533, 3599, 3635
BtgI CCRYGG 3 cut(s) 172, 1703, 1779
BtsI GCAGTG 1 cut(s) 18
BveI ACCTGC 1 cut(s) 1614
Cac8I GCNNGC 5 cut(s) 1010, 1397, 2428, 3148, 3478
CaiI CAGNNNCTG 2 cut(s) 158, 1721
CciI TCATGA 2 cut(s) 1147, 1225
CfoI GCGC 2 cut(s) 71, 2548
Cfr13I GGNCC 7 cut(s) 155, 726, 2066, 2801, 3267, 3495, 3597
Cfr42I CCGCGG 1 cut(s) 175
CseI GACGC 1 cut(s) 484
CsiI ACCWGGT 1 cut(s) 2803
Csp6I GTAC 8 cut(s) 519, 1162, 1322, 1469, 1557, 2026, 2031, 2360
CviQI GTAC 8 cut(s) 519, 1162, 1322, 1469, 1557, 2026, 2031, 2360
DraI TTTAAA 1 cut(s) 3336
EaeI YGGCCR 1 cut(s) 983
EagI CGGCCG 1 cut(s) 983
Eam1104I CTCTTC 4 cut(s) 399, 1574, 2404, 2524
EarI CTCTTC 4 cut(s) 399, 1574, 2404, 2524
EclXI CGGCCG 1 cut(s) 983
Eco130I CCWWGG 4 cut(s) 45, 798, 1703, 1779
Eco147I AGGCCT 1 cut(s) 2533
Eco24I GRGCYC 3 cut(s) 914, 1101, 2195
Eco31I GGTCTC 1 cut(s) 2583
Eco32I GATATC 2 cut(s) 2348, 2994
Eco47I GGWCC 5 cut(s) 726, 2066, 2801, 3267, 3495
Eco52I CGGCCG 1 cut(s) 983
Eco57I CTGAAG 3 cut(s) 2351, 2385, 3466
Eco72I CACGTG 1 cut(s) 2731
Eco81I CCTNAGG 1 cut(s) 2583
Eco88I CYCGRG 3 cut(s) 1449, 2090, 3323
EcoNI CCTNNNNNAGG 1 cut(s) 3217
EcoO109I RGGNCCY 1 cut(s) 155
EcoRI GAATTC 2 cut(s) 1909, 3233
EcoRII CCWGG 7 cut(s) 320, 1122, 1399, 2803, 3063, 3299, 3635
EcoRV GATATC 2 cut(s) 2348, 2994
EcoT14I CCWWGG 4 cut(s) 45, 798, 1703, 1779
EcoT22I ATGCAT 2 cut(s) 975, 2528
EcoT38I GRGCYC 3 cut(s) 914, 1101, 2195
ErhI CCWWGG 4 cut(s) 45, 798, 1703, 1779
Esp3I CGTCTC 1 cut(s) 1822
FalI AAGNNNNNCTT 4 cut(s) 1475, 1507, 2349, 2381
FaqI GGGAC 1 cut(s) 3122
FauNDI CATATG 1 cut(s) 1085
FbaI TGATCA 2 cut(s) 601, 2478
FblI GTMKAC 1 cut(s) 1138
Fnu4HI GCNGC 6 cut(s) 175, 2450, 3122, 3207, 3224, 3436
FriOI GRGCYC 3 cut(s) 914, 1101, 2195
Fsp4HI GCNGC 6 cut(s) 175, 2450, 3122, 3207, 3224, 3436
FspBI CTAG 5 cut(s) 948, 1353, 3071, 3158, 3507
GlaI GCGC 2 cut(s) 70, 2547
GluI GCNGC 6 cut(s) 175, 2450, 3122, 3207, 3224, 3436
GsuI CTGGAG 3 cut(s) 95, 1437, 3322
HaeIII GGCC 6 cut(s) 156, 985, 1895, 2533, 3599, 3635
HapII CCGG 4 cut(s) 329, 868, 986, 1275
HgaI GACGC 1 cut(s) 484
HhaI GCGC 2 cut(s) 71, 2548
Hin1I GRCGYC 2 cut(s) 476, 2372
Hin6I GCGC 2 cut(s) 69, 2546
HinP1I GCGC 2 cut(s) 69, 2546
HincII GTYRAC 3 cut(s) 597, 1372, 1662
HindII GTYRAC 3 cut(s) 597, 1372, 1662
HindIII AAGCTT 1 cut(s) 1643
HpaI GTTAAC 2 cut(s) 597, 1662
HpaII CCGG 4 cut(s) 329, 868, 986, 1275
HphI GGTGA 5 cut(s) 128, 1075, 1498, 1595, 1664
Hpy166II GTNNAC 9 cut(s) 597, 953, 1139, 1372, 1489, 1507, 1662, 2075, 2183
Hpy8I GTNNAC 9 cut(s) 597, 953, 1139, 1372, 1489, 1507, 1662, 2075, 2183
Hpy99I CGWCG 1 cut(s) 1901
HpyCH4IV ACGT 3 cut(s) 2372, 2730, 2819
HpyF10VI GCNNNNNNNGC 4 cut(s) 174, 3203, 3223, 3583
HpySE526I ACGT 3 cut(s) 2372, 2730, 2819
Hsp92I GRCGYC 2 cut(s) 476, 2372
HspAI GCGC 2 cut(s) 69, 2546
Kpn2I TCCGGA 2 cut(s) 867, 1274
Ksp22I TGATCA 2 cut(s) 601, 2478
KspAI GTTAAC 2 cut(s) 597, 1662
KspI CCGCGG 1 cut(s) 175
LmnI GCTCC 2 cut(s) 2247, 2845
Lsp1109I GCAGC 5 cut(s) 2436, 3133, 3210, 3218, 3447
LweI GCATC 8 cut(s) 334, 948, 982, 1285, 1336, 2803, 3133, 3514
MabI ACCWGGT 1 cut(s) 2803
MaeI CTAG 5 cut(s) 948, 1353, 3071, 3158, 3507
MaeII ACGT 3 cut(s) 2372, 2730, 2819
MflI RGATCY 4 cut(s) 1187, 1212, 2859, 3397
MhlI GDGCHC 6 cut(s) 643, 914, 1101, 1117, 2195, 2933
MlyI GAGTC 6 cut(s) 86, 236, 416, 824, 1051, 1931
MmeI TCCRAC 3 cut(s) 1777, 1879, 2773
Mph1103I ATGCAT 2 cut(s) 975, 2528
MroI TCCGGA 2 cut(s) 867, 1274
MroXI GAANNNNTTC 3 cut(s) 2361, 3003, 3450
MslI CAYNNNNRTG 3 cut(s) 1106, 2296, 2729
MspA1I CMGCKG 2 cut(s) 174, 2125
MspCI CTTAAG 1 cut(s) 2186
MspI CCGG 4 cut(s) 329, 868, 986, 1275
MspR9I CCNGG 8 cut(s) 322, 987, 1124, 1401, 2805, 3065, 3301, 3637
Mva1269I GAATGC 2 cut(s) 705, 2724
MvaI CCWGG 7 cut(s) 322, 1124, 1401, 2805, 3065, 3301, 3637
MvnI CGCG 1 cut(s) 174
MwoI GCNNNNNNNGC 4 cut(s) 174, 3203, 3223, 3583
NciI CCSGG 1 cut(s) 987
NcoI CCATGG 2 cut(s) 1703, 1779
NdeI CATATG 1 cut(s) 1085
NlaIV GGNNCC 1 cut(s) 2249
NmeAIII GCCGAG 1 cut(s) 961
NmuCI GTSAC 3 cut(s) 1088, 2327, 2491
NsiI ATGCAT 2 cut(s) 975, 2528
NspI RCATGY 5 cut(s) 1399, 1743, 2410, 2615, 2953
NspV TTCGAA 1 cut(s) 128
PaeI GCATGC 1 cut(s) 1399
PaeR7I CTCGAG 3 cut(s) 1449, 2090, 3323
PagI TCATGA 2 cut(s) 1147, 1225
PaqCI CACCTGC 1 cut(s) 1614
PceI AGGCCT 1 cut(s) 2533
PciI ACATGT 1 cut(s) 2406
PctI GAATGC 2 cut(s) 705, 2724
PdmI GAANNNNTTC 3 cut(s) 2361, 3003, 3450
PflMI CCANNNNNTGG 5 cut(s) 193, 471, 840, 3064, 3146
PfoI TCCNGGA 1 cut(s) 3299
PkrI GCNGC 6 cut(s) 176, 2451, 3123, 3208, 3225, 3437
PleI GAGTC 6 cut(s) 85, 235, 415, 823, 1051, 1930
PmaCI CACGTG 1 cut(s) 2731
PmlI CACGTG 1 cut(s) 2731
PpsI GAGTC 6 cut(s) 85, 235, 415, 823, 1051, 1930
Ppu21I YACGTR 1 cut(s) 2731
PscI ACATGT 1 cut(s) 2406
PshAI GACNNNNGTC 1 cut(s) 2409
PsiI TTATAA 1 cut(s) 2115
Psp1406I AACGTT 1 cut(s) 2819
Psp6I CCWGG 7 cut(s) 320, 1122, 1399, 2803, 3063, 3299, 3635
PspCI CACGTG 1 cut(s) 2731
PspGI CCWGG 7 cut(s) 320, 1122, 1399, 2803, 3063, 3299, 3635
PspN4I GGNNCC 1 cut(s) 2249
PspPI GGNCC 7 cut(s) 155, 726, 2066, 2801, 3267, 3495, 3597
PspXI VCTCGAGB 2 cut(s) 1449, 2090
PstI CTGCAG 1 cut(s) 1287
PstNI CAGNNNCTG 2 cut(s) 158, 1721
PsuI RGATCY 4 cut(s) 1187, 1212, 2859, 3397
PvuII CAGCTG 1 cut(s) 2125
RsaI GTAC 8 cut(s) 520, 1163, 1323, 1470, 1558, 2027, 2032, 2361
RsaNI GTAC 8 cut(s) 519, 1162, 1322, 1469, 1557, 2026, 2031, 2360
RseI CAYNNNNRTG 3 cut(s) 1106, 2296, 2729
SacII CCGCGG 1 cut(s) 175
SatI GCNGC 6 cut(s) 175, 2450, 3122, 3207, 3224, 3436
Sau96I GGNCC 7 cut(s) 155, 726, 2066, 2801, 3267, 3495, 3597
ScaI AGTACT 1 cut(s) 2361
SchI GAGTC 6 cut(s) 86, 236, 416, 824, 1051, 1931
ScrFI CCNGG 8 cut(s) 322, 987, 1124, 1401, 2805, 3065, 3301, 3637
SduI GDGCHC 6 cut(s) 643, 914, 1101, 1117, 2195, 2933
SexAI ACCWGGT 1 cut(s) 2803
SfaNI GCATC 8 cut(s) 334, 948, 982, 1285, 1336, 2803, 3133, 3514
SfcI CTRYAG 3 cut(s) 429, 1283, 1375
Sfr274I CTCGAG 3 cut(s) 1449, 2090, 3323
Sfr303I CCGCGG 1 cut(s) 175
SfuI TTCGAA 1 cut(s) 128
SgrBI CCGCGG 1 cut(s) 175
SinI GGWCC 5 cut(s) 726, 2066, 2801, 3267, 3495
SlaI CTCGAG 3 cut(s) 1449, 2090, 3323
SmiMI CAYNNNNRTG 3 cut(s) 1106, 2296, 2729
SmlI CTYRAG 9 cut(s) 1449, 1516, 1963, 2090, 2186, 2687, 2967, 3115, 3323
SmoI CTYRAG 9 cut(s) 1449, 1516, 1963, 2090, 2186, 2687, 2967, 3115, 3323
SphI GCATGC 1 cut(s) 1399
SseBI AGGCCT 1 cut(s) 2533
SsiI CCGC 5 cut(s) 172, 174, 306, 2064, 2621
SspI AATATT 1 cut(s) 355
SspMI CTAG 5 cut(s) 948, 1353, 3071, 3158, 3507
StuI AGGCCT 1 cut(s) 2533
StyD4I CCNGG 8 cut(s) 320, 985, 1122, 1399, 2803, 3063, 3299, 3635
StyI CCWWGG 4 cut(s) 45, 798, 1703, 1779
TaiI ACGT 3 cut(s) 2375, 2733, 2822
TaqI TCGA 5 cut(s) 128, 813, 1450, 2091, 3324
TatI WGTACW 3 cut(s) 1161, 1556, 2359
TauI GCSGC 1 cut(s) 177
TseFI GTSAC 3 cut(s) 1088, 2327, 2491
TseI GCWGC 5 cut(s) 2449, 3121, 3206, 3223, 3435
Tsp45I GTSAC 3 cut(s) 1088, 2327, 2491
TspGWI ACGGA 2 cut(s) 1515, 1846
Van91I CCANNNNNTGG 5 cut(s) 193, 471, 840, 3064, 3146
Vha464I CTTAAG 1 cut(s) 2186
VpaK11BI GGWCC 5 cut(s) 726, 2066, 2801, 3267, 3495
XagI CCTNNNNNAGG 1 cut(s) 3217
XbaI TCTAGA 2 cut(s) 3070, 3157
XceI RCATGY 5 cut(s) 1399, 1743, 2410, 2615, 2953
XcmI CCANNNNNNNNNTGG 3 cut(s) 736, 2897, 3183
XhoI CTCGAG 3 cut(s) 1449, 2090, 3323
XmiI GTMKAC 1 cut(s) 1138
XmnI GAANNNNTTC 3 cut(s) 2361, 3003, 3450
XspI CTAG 5 cut(s) 948, 1353, 3071, 3158, 3507
ZraI GACGTC 1 cut(s) 2373
ZrmI AGTACT 1 cut(s) 2361
Zsp2I ATGCAT 2 cut(s) 975, 2528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.