Rroxscaffold_3G00234500

CST complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0006351
Physical Location & Seq
Reverse (-)
20920596 .. 20927797
7202 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00234500.1

Sequence Viewer

Length: 4143 bp
ATGGAAAATGTGAGAGTCTTGACAATCGCAGACTTACTCCAACGCGGCCGTTGCCTCACCGGAACCTCCTCTTTCGGTACCCCTTCTTCCTTTGCTCCAAAATCAAACCCCTCCATTTCTCCCATCCCCTCAAACCCTAACCCTTATTCCACCACTAAGGTTCTCCCCTCTCTCGACCACTTTGCCCTCATCATCGGAACTATAACTCTCCCCAGCGCCACCTCCGCCTCCACCTCCGCTTCAAACGCCGCCGCCGCTCCTCGATGCTCCTACAACACCTGCTTCCAATTCTCAGACAACTCGGCCGCCTCGATCTGCTGCGACGTTCTTGATTTCGATGCTCGGATTCTCGACAAGAGAATCAATGTTCTCGCGTGGAATTTCATTCCGTTCAAACGTGGCGGTGGGTTCTTGGAGATTATCAGATGGGGGTTTCAGGATTCAATAGGTGGAATTTGGCCGTGTTCGAATATCATTTCTATGGATTTAGGCTCTTCCACTGCTAATGAAGACAGTTCCAAGGCTCGTCATGGTGTGTTCGGCGCACTGGAGTCGGTTAGCCCTTCGTCCATTGTTTCATGTAGAACAGGTGATAGCAATTCGAAAAGCAAAGAGGTTTTCCATTCAGGCCCTCTGACAAGCCTCCGCGGCTTCTTAGTCCAATTTATGGTTTGTGAGTGTAGATCATGCAGTTCAAAGGAGTCAATCATGGTTTTGAAGGATTCAATTCAAGAAAGAGATACTCATTCTTTTACCAAACCGATGTTTGTATATTGTTGCGGTTCTGCTTCATCCTGGCATCCTGTATTTACGAAACTTGTGGGAAATATTGTTGCTATATCAGGCTTGAAAAAGAAGTTGGTTTTTCTAGGGAAGGAAGAGTCTAAAGTAATGTGTGTAACTACAGGAAGTTCTGCCTTGCATTTGAGCAGATTGTCCAAGAAATGGACGCCGAAAGTTACAGTAGGTAGAAAGGGAAATGGGGAAGTTGGTTCATATAGAGGGATAGTTAAGGGTGTGTATATGCAAGGAATGGTTGTGGAATTGGACAATGAGGTGTGGCTTTTGTTAACTGATCAACTGCTTACTCCACCACATAGTCTTAGGGCGGGTGCTCTTGTTTCTGTGAGAAATGCCCATTTTGTAAATCCCAGATTTTCTTGGACAAGAAGAATGCTTATACTTGGTGCTTGCTTTAGGACCAATGTAATTGTGGAATCCTTCTCTCCAATAGAAACTCGGTGTCATATAGCCTCACAATCACAGAGTTCCTTGGGGAAGTTCATTGAGTCCTTAGCATTTTCTTCCAGATTATGGGTATTGCTGGTTGCCTCATATTTCCGGAAAAAGTTTGCTGGTATCTTATCTGAGAAGGAAATTTTGGGCTCAAAACATAAGGGAGGATTAGTTGAGATGTTTGCTAGTTCACAGATGCCTTCGTCAAAGCATCAAACTCGGCCGGGAGTATTCATGGAATTTTGCAAGCATGATTCCTGTGCTTGTGGTTGTGAGCCATACATAGATAATCTGACTCTGGCGATCCCCTTGTCTTTTTTCATATGTCACTGTGAGCCCACATGGATGAGGGCACTGAACCTGGAAGGTAACAGTAGAAAATTGCATGATGACAAACAGTACAGCCGTCAATTATGTGAAGGGAGATCTTATGTTCAGTCTATTAGAAAGATCTTTTCAAGTGAAGATATAGGCATAACTTTGATTGGAAGTTTGAAGACTTCTCCATCTTCCGGAAGACTGCAGTTGGTTGATGCAACTGGTAGCATTGATGTTCTTGTACCAGACCTTCCATCAACTTGGGATGCCACTAGAATAATCAAGGTAGTTGACTACAGTGTAATTATTGAAGGCATGCCTGGGTTTGTGGATTCCGAAGGATTTCTTGAATATGATTTGTTCTCAACTCGAACTATCTTTGATTTTGTACCATTGGCAAGAAAGGTGAACTTAACAGTTTGTGTTTACTTTCGCTTGAGAAGTCCATTGTGCAGAAATCTTTGCTTTTATCCTTGTACTGGATTGGGAGAAGATTTGAAGGGATTTGAAAGTGGAACATTTCACCTGCTATGGATTACCCACAAATTTCCTGTGCTACACAAGTTTCAAGGTGATGCGTTAACAAGCTCATCAAGCATGTTTGTTGAGGCTATAATCTTGCCATGGAATCTATCAGTTGCTGGAAACAATCATATTTCGTGTCATACTGGGGTAGTTGGGGATGATCCAAAAAATTCCATGGAATTTTGTGCTGTTGGATGTTACTTGAAGAATGGTTCTTTCAAGAGACAGAAGGTTTGTGATTCATCAATGAAGGAACTAAGTTTTGGTTCAATGGACTGTTCCTGTGAGGCCGTTGGAAAACTGAATTCTTGTTCTGAGTCTTACATTGAATCCAGTGAGGATAAAACATATAGCGACTTGAGTTCTCATGAAATATCTGGTTTGGCTATAATTAGTGGAGTTACTCGTTCAGTTATCTTGTACCGTACAAAAGCCAAATTAAAAAGTGGTAGCTTTTGCGGTCCAAGTGGACAGAAGATAATGCTTGAGTTCAAGTCTGACAGCTTTTATAAGTATCAGCTGTTGCAGATTGGCTGTTATTATATCACAAAGCATGATGGAGAAGATTATTTTTGTAATCTTAAGGGCTCTGACTATTTCATTGGTCAAAAAATTCTCATCCCTCCCACAACTCATCTGTGGAGCCTTTCATTTGGTTCTGATGAGGCTTGTCAAAATAACAGTTCATCAAAGTGTATTCCATTGGATGATTCTCTTAGAAGTGACGAGGTTCTATCTGGATATCATAACGAAGTACTTCTTCAGACATCCAATGAAAATTTGTCTGAAACCTCTTCAGACATCTGTCTTTGTCTGTCTGCAAGCGTATTAGGTCTTGGAGAGCTGCACCTAAAGGAACTGAAAGAAAGCCTGATCAAGCCAGTTGTGACACCTAAAGACATTCCAAAAATCTCTTCATGCATTAGGCCTGTAACAACTGCGCCTCCACTATCAACTGAATCTAACCGTATGTTTCCTGAGGGAGACCTAATATCTATGCGTGGGCATGTGGTTGCGGTTCATAGCATTGAGGACAACTCTGTTGATCCATATTTGAACCGTCAAAACCTTCGTGATCCTCTTGAGTTGAGATTCTTGCAGAGGACAACAAGCAGTTGCATTCACGTGTTGGTGGATAATCAAATTGTAAAGCTATCTGGTTCTTTGCGCAAACATGATTTTCCAGTCGGATTTGGACCTGGTGTGGATGCAATGTTTTATCGGATTCTTGCACTGTGGGAGCAGAATAGATGGATCTTGACATCGGTATCATTTATTACCATCCATTCCATAAGTGGAGACAATGAATCATGTGGTGTCAAGTGCTCTAATCCTGCTTCATACATGCCCAGTGCTTCGCCTCAAGAGATTGTCCATTCAGGGTTGATATCTGAATTGGTTCAAAGTTTGGACTTCAAGCCAGTGCTGTTGCATTGCAGGGTTGTTGCTATCCATTTCCTGGTTCTAGAGAAGAAAAGTAGAAATGTCAATTATCAACTAAAAAATCACTTGAGGCAGCATCTTGTTGATGTCCCACTAGCTGGCTTTGTTCTAGATGATGGTTCTTCTCCATGCTGTTGTTGGGCTAATGCTGAAAGAGCAGCAACCTTGCTGAGGCTGCATGAAGAATTCCCACAGTTAGCTTTTGAAAATAGTGGCTGGACCTTAAAGTGGGTCAGAACAAACAATAATTCCTGGAGTTCCACTATCTATCATCTTGAGAGAATTTTAAATAATCATCATCGGATTGTAGTGAGGAACTATGGACCAATGTTTGATTCGTCTTATCAAGATCTTGCTGTGTCTGTGAGTTCAGATAATGCCCTAAGCAGCTATGATGAAAACCTTCTAAAGTTTATAGTCTTCAATGCTTGCTTCAGCAAATTTTGGACCATTGGTGCTAGTATGATGGATTTGGATGCTGTCAGGCTATTGAAGACAGAGAACCTTGTGGAAATGGAGATGTCTATGCAATCCATGCAACATATATGGGCCACAGAAGTTCAACACATCAACCATCTGAGTCAGGCCAGGAATGTTATTCAAGAACTGATAGACGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CTC1_2

Protein Analysis

1380

Amino Acids

153.08

Weight (kDa)

7.46

Isoelectric Point (pI)

44.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CTC1_2 PF15491 58 - 372 9.2e-93 CST, telomere maintenance, complex subunit CTC1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2589
AarI CACCTGC 2 cut(s) 287, 2088
Acc16I TGCGCA 1 cut(s) 3248
Acc36I ACCTGC 2 cut(s) 287, 2088
Acc65I GGTACC 1 cut(s) 77
AccB1I GGYRCC 1 cut(s) 77
AccB7I CCANNNNNTGG 5 cut(s) 667, 945, 1314, 3538, 3620
AccBSI CCGCTC 1 cut(s) 257
AccII CGCG 3 cut(s) 45, 374, 648
AccIII TCCGGA 2 cut(s) 1341, 1748
AclWI GGATC 5 cut(s) 1534, 2234, 3119, 3149, 3341
AcoI YGGCCR 4 cut(s) 46, 303, 458, 1457
AcuI CTGAAG 3 cut(s) 2825, 2859, 3940
AcvI CACGTG 1 cut(s) 3205
AcyI GRCGYC 1 cut(s) 950
AfaI GTAC 8 cut(s) 79, 1637, 1797, 1944, 2032, 2501, 2506, 2835
AfiI CCNNNNNNNGG 9 cut(s) 667, 945, 1314, 1748, 2033, 3538, 3620, 3693, 3750
AflII CTTAAG 1 cut(s) 2660
AflIII ACRYGT 1 cut(s) 3204
AjnI CCWGG 7 cut(s) 794, 1596, 1873, 3277, 3537, 3773, 4109
AluBI AGCT 9 cut(s) 2142, 2532, 2583, 2599, 2923, 3232, 3620, 3722, 3912
AluI AGCT 9 cut(s) 2142, 2532, 2583, 2599, 2923, 3232, 3620, 3722, 3912
Alw21I GWGCWC 2 cut(s) 1117, 3407
Alw26I GTCTC 3 cut(s) 2296, 3057, 3372
AlwI GGATC 5 cut(s) 1534, 2234, 3119, 3149, 3341
AlwNI CAGNNNCTG 1 cut(s) 2195
Aor13HI TCCGGA 2 cut(s) 1341, 1748
AoxI GGCC 9 cut(s) 46, 303, 458, 628, 1457, 2367, 3005, 4071, 4107
ApeKI GCWGC 6 cut(s) 318, 2923, 3595, 3680, 3697, 3909
ArsI GACNNNNNNTTYG 2 cut(s) 2295, 2327
Asp700I GAANNNNTTC 4 cut(s) 1896, 2835, 3477, 3924
Asp718I GGTACC 1 cut(s) 77
AspLEI GCGC 4 cut(s) 218, 545, 3022, 3249
AspS9I GGNCC 8 cut(s) 629, 1200, 2540, 3275, 3741, 3845, 3969, 4071
AsuC2I CCSGG 1 cut(s) 1461
AsuHPI GGTGA 5 cut(s) 49, 602, 1972, 2069, 2138
AsuII TTCGAA 2 cut(s) 467, 602
AvaII GGWCC 6 cut(s) 1200, 2540, 3275, 3741, 3845, 3969
AxyI CCTNAGG 1 cut(s) 3057
BaeGI GKGCMC 1 cut(s) 1591
BaeI ACNNNNGTAYC 2 cut(s) 3330, 3363
BanI GGYRCC 1 cut(s) 77
BanII GRGCYC 3 cut(s) 1388, 1575, 2669
BarI GAAGNNNNNNTAC 4 cut(s) 70, 102, 1788, 1820
BbrPI CACGTG 1 cut(s) 3205
BbsI GAAGAC 5 cut(s) 516, 1739, 1759, 3934, 4022
Bbv12I GWGCWC 2 cut(s) 1117, 3407
BbvCI CCTCAGC 1 cut(s) 3692
BbvI GCAGC 6 cut(s) 305, 2910, 3607, 3684, 3692, 3921
BceAI ACGGC 4 cut(s) 33, 445, 1626, 2354
BcgI CGANNNNNNTGC 6 cut(s) 164, 198, 534, 568, 1436, 1470
BciT130I CCWGG 7 cut(s) 796, 1598, 1875, 3279, 3539, 3775, 4111
BclI TGATCA 2 cut(s) 1075, 2952
BcnI CCSGG 1 cut(s) 1461
BcoDI GTCTC 3 cut(s) 2296, 3057, 3372
BfaI CTAG 7 cut(s) 869, 1422, 1827, 3545, 3617, 3632, 3981
BfmI CTRYAG 3 cut(s) 903, 1757, 1849
BfoI RGCGCY 1 cut(s) 219
BfrI CTTAAG 1 cut(s) 2660
BfuAI ACCTGC 2 cut(s) 287, 2088
BglI GCCNNNNNGGC 1 cut(s) 648
BglII AGATCT 3 cut(s) 1661, 1686, 3871
BmcAI AGTACT 1 cut(s) 2835
Bme1390I CCNGG 8 cut(s) 796, 1461, 1598, 1875, 3279, 3539, 3775, 4111
Bme18I GGWCC 6 cut(s) 1200, 2540, 3275, 3741, 3845, 3969
BmgT120I GGNCC 8 cut(s) 629, 1200, 2540, 3275, 3741, 3845, 3969, 4071
BmiI GGNNCC 3 cut(s) 64, 79, 2723
BmrFI CCNGG 8 cut(s) 796, 1461, 1598, 1875, 3279, 3539, 3775, 4111
BmrI ACTGGG 2 cut(s) 2232, 3423
BmuI ACTGGG 2 cut(s) 2232, 3423
BoxI GACNNNNGTC 1 cut(s) 2883
BpiI GAAGAC 5 cut(s) 516, 1739, 1759, 3934, 4022
BplI GAGNNNNNCTC 2 cut(s) 3101, 3133
BpmI CTGGAG 2 cut(s) 569, 3796
Bpu10I CCTNAGC 3 cut(s) 1294, 3692, 3905
Bpu14I TTCGAA 2 cut(s) 467, 602
BpuEI CTTGAG 7 cut(s) 2011, 2458, 2585, 3182, 3426, 3610, 3818
BpuMI CCSGG 1 cut(s) 1461
BsaAI YACGTR 1 cut(s) 3205
BsaHI GRCGYC 1 cut(s) 950
BsaI GGTCTC 1 cut(s) 3057
BsaJI CCNNGG 6 cut(s) 519, 646, 1272, 1874, 2177, 2253
BsaWI WCCGGW 3 cut(s) 59, 1341, 1748
BsaXI ACNNNNNCTCC 4 cut(s) 1392, 1422, 3007, 3037
Bsc4I CCNNNNNNNGG 9 cut(s) 667, 945, 1314, 1748, 2033, 3538, 3620, 3693, 3750
Bse1I ACTGG 9 cut(s) 552, 1780, 2038, 2227, 2412, 2960, 3263, 3429, 3500
Bse21I CCTNAGG 1 cut(s) 3057
Bse3DI GCAATG 2 cut(s) 3297, 3511
BseAI TCCGGA 2 cut(s) 1341, 1748
BseBI CCWGG 7 cut(s) 796, 1598, 1875, 3279, 3539, 3775, 4111
BseDI CCNNGG 6 cut(s) 519, 646, 1272, 1874, 2177, 2253
BseLI CCNNNNNNNGG 9 cut(s) 667, 945, 1314, 1748, 2033, 3538, 3620, 3693, 3750
BseMI GCAATG 2 cut(s) 3297, 3511
BseMII CTCAG 6 cut(s) 306, 1359, 2385, 3048, 3683, 4091
BseNI ACTGG 9 cut(s) 552, 1780, 2038, 2227, 2412, 2960, 3263, 3429, 3500
BseRI GAGGAG 2 cut(s) 58, 249
BseSI GKGCMC 1 cut(s) 1591
BseX3I CGGCCG 3 cut(s) 46, 303, 1457
BseXI GCAGC 6 cut(s) 305, 2910, 3607, 3684, 3692, 3921
BseYI CCCAGC 1 cut(s) 212
BsgI GTGCAG 2 cut(s) 2026, 2909
Bsh1236I CGCG 3 cut(s) 45, 374, 648
Bsh1285I CGRYCG 3 cut(s) 49, 306, 1460
BshFI GGCC 9 cut(s) 48, 305, 460, 630, 1459, 2369, 3007, 4073, 4109
BshNI GGYRCC 1 cut(s) 77
BsiEI CGRYCG 3 cut(s) 49, 306, 1460
BsiHKAI GWGCWC 2 cut(s) 1117, 3407
BsiSI CCGG 4 cut(s) 60, 1342, 1460, 1749
BslFI GGGAC 1 cut(s) 3596
BslI CCNNNNNNNGG 9 cut(s) 667, 945, 1314, 1748, 2033, 3538, 3620, 3693, 3750
BsmAI GTCTC 3 cut(s) 2296, 3057, 3372
BsmFI GGGAC 1 cut(s) 3596
BsmI GAATGC 2 cut(s) 1179, 3198
BsnI GGCC 9 cut(s) 48, 305, 460, 630, 1459, 2369, 3007, 4073, 4109
Bso31I GGTCTC 1 cut(s) 3057
Bsp119I TTCGAA 2 cut(s) 467, 602
Bsp1286I GDGCHC 6 cut(s) 1117, 1388, 1575, 1591, 2669, 3407
Bsp13I TCCGGA 2 cut(s) 1341, 1748
Bsp19I CCATGG 2 cut(s) 2177, 2253
BspANI GGCC 9 cut(s) 48, 305, 460, 630, 1459, 2369, 3007, 4073, 4109
BspCNI CTCAG 6 cut(s) 305, 1360, 2386, 3049, 3684, 4092
BspEI TCCGGA 2 cut(s) 1341, 1748
BspFNI CGCG 3 cut(s) 45, 374, 648
BspHI TCATGA 1 cut(s) 2446
BspLI GGNNCC 3 cut(s) 64, 79, 2723
BspMAI CTGCAG 1 cut(s) 1761
BspMI ACCTGC 2 cut(s) 287, 2088
BspPI GGATC 5 cut(s) 1534, 2234, 3119, 3149, 3341
BspQI GCTCTTC 1 cut(s) 499
BspT104I TTCGAA 2 cut(s) 467, 602
BspT107I GGYRCC 1 cut(s) 77
BspTI CTTAAG 1 cut(s) 2660
BspTNI GGTCTC 1 cut(s) 3057
BsrBI CCGCTC 1 cut(s) 257
BsrDI GCAATG 2 cut(s) 3297, 3511
BsrI ACTGG 9 cut(s) 552, 1780, 2038, 2227, 2412, 2960, 3263, 3429, 3500
BssECI CCNNGG 6 cut(s) 519, 646, 1272, 1874, 2177, 2253
BssNI GRCGYC 1 cut(s) 950
BssT1I CCWWGG 4 cut(s) 519, 1272, 2177, 2253
Bst2UI CCWGG 7 cut(s) 796, 1598, 1875, 3279, 3539, 3775, 4111
Bst6I CTCTTC 4 cut(s) 499, 873, 2878, 2998
BstACI GRCGYC 1 cut(s) 950
BstAFI CTTAAG 1 cut(s) 2660
BstAPI GCANNNNNTGC 1 cut(s) 4057
BstBAI YACGTR 1 cut(s) 3205
BstBI TTCGAA 2 cut(s) 467, 602
BstC8I GCNNGC 6 cut(s) 1192, 1484, 1871, 2902, 3622, 3952
BstDSI CCRYGG 3 cut(s) 646, 2177, 2253
BstENI CCTNNNNNAGG 1 cut(s) 3691
BstFNI CGCG 3 cut(s) 45, 374, 648
BstH2I RGCGCY 1 cut(s) 219
BstHHI GCGC 4 cut(s) 218, 545, 3022, 3249
BstMAI GTCTC 3 cut(s) 2296, 3057, 3372
BstMCI CGRYCG 3 cut(s) 49, 306, 1460
BstMWI GCNNNNNNNGC 9 cut(s) 51, 224, 245, 254, 648, 2148, 3677, 3697, 4057
BstNI CCWGG 7 cut(s) 796, 1598, 1875, 3279, 3539, 3775, 4111
BstNSI RCATGY 4 cut(s) 1873, 2155, 3089, 3427
BstPAI GACNNNNGTC 1 cut(s) 2883
BstSCI CCNGG 8 cut(s) 794, 1459, 1596, 1873, 3277, 3537, 3773, 4109
BstSFI CTRYAG 3 cut(s) 903, 1757, 1849
BstSLI GKGCMC 1 cut(s) 1591
BstUI CGCG 3 cut(s) 45, 374, 648
BstV1I GCAGC 6 cut(s) 305, 2910, 3607, 3684, 3692, 3921
BstV2I GAAGAC 5 cut(s) 516, 1739, 1759, 3934, 4022
BstX2I RGATCY 4 cut(s) 1661, 1686, 3333, 3871
BstXI CCANNNNNNTGG 1 cut(s) 1815
BstYI RGATCY 4 cut(s) 1661, 1686, 3333, 3871
BstZI CGGCCG 3 cut(s) 46, 303, 1457
Bsu36I CCTNAGG 1 cut(s) 3057
BsuRI GGCC 9 cut(s) 48, 305, 460, 630, 1459, 2369, 3007, 4073, 4109
BtgI CCRYGG 3 cut(s) 646, 2177, 2253
BtsI GCAGTG 1 cut(s) 498
BtsIMutI CAGTG 9 cut(s) 498, 545, 1564, 1589, 1858, 2419, 3311, 3436, 3507
BveI ACCTGC 2 cut(s) 287, 2088
Cac8I GCNNGC 6 cut(s) 1192, 1484, 1871, 2902, 3622, 3952
CaiI CAGNNNCTG 1 cut(s) 2195
CciI TCATGA 1 cut(s) 2446
CfoI GCGC 4 cut(s) 218, 545, 3022, 3249
Cfr13I GGNCC 8 cut(s) 629, 1200, 2540, 3275, 3741, 3845, 3969, 4071
Cfr42I CCGCGG 1 cut(s) 649
CseI GACGC 1 cut(s) 958
CsiI ACCWGGT 1 cut(s) 3277
Csp6I GTAC 8 cut(s) 78, 1636, 1796, 1943, 2031, 2500, 2505, 2834
CviQI GTAC 8 cut(s) 78, 1636, 1796, 1943, 2031, 2500, 2505, 2834
DraI TTTAAA 1 cut(s) 3810
EaeI YGGCCR 4 cut(s) 46, 303, 458, 1457
EagI CGGCCG 3 cut(s) 46, 303, 1457
Eam1104I CTCTTC 4 cut(s) 499, 873, 2878, 2998
EarI CTCTTC 4 cut(s) 499, 873, 2878, 2998
EciI GGCGGA 1 cut(s) 214
EclXI CGGCCG 3 cut(s) 46, 303, 1457
Eco130I CCWWGG 4 cut(s) 519, 1272, 2177, 2253
Eco147I AGGCCT 1 cut(s) 3007
Eco24I GRGCYC 3 cut(s) 1388, 1575, 2669
Eco31I GGTCTC 1 cut(s) 3057
Eco32I GATATC 2 cut(s) 2822, 3468
Eco47I GGWCC 6 cut(s) 1200, 2540, 3275, 3741, 3845, 3969
Eco52I CGGCCG 3 cut(s) 46, 303, 1457
Eco57I CTGAAG 3 cut(s) 2825, 2859, 3940
Eco72I CACGTG 1 cut(s) 3205
Eco81I CCTNAGG 1 cut(s) 3057
EcoNI CCTNNNNNAGG 1 cut(s) 3691
EcoO109I RGGNCCY 1 cut(s) 629
EcoRI GAATTC 2 cut(s) 2383, 3707
EcoRII CCWGG 7 cut(s) 794, 1596, 1873, 3277, 3537, 3773, 4109
EcoRV GATATC 2 cut(s) 2822, 3468
EcoT14I CCWWGG 4 cut(s) 519, 1272, 2177, 2253
EcoT22I ATGCAT 1 cut(s) 3002
EcoT38I GRGCYC 3 cut(s) 1388, 1575, 2669
ErhI CCWWGG 4 cut(s) 519, 1272, 2177, 2253
FalI AAGNNNNNCTT 6 cut(s) 1884, 1916, 1949, 1981, 2823, 2855
FaqI GGGAC 1 cut(s) 3596
FauI CCCGC 1 cut(s) 1102
FauNDI CATATG 1 cut(s) 1559
FbaI TGATCA 2 cut(s) 1075, 2952
FriOI GRGCYC 3 cut(s) 1388, 1575, 2669
FspBI CTAG 7 cut(s) 869, 1422, 1827, 3545, 3617, 3632, 3981
FspI TGCGCA 1 cut(s) 3248
GlaI GCGC 4 cut(s) 217, 544, 3021, 3248
GsaI CCCAGC 1 cut(s) 216
GsuI CTGGAG 2 cut(s) 569, 3796
HaeII RGCGCY 1 cut(s) 219
HaeIII GGCC 9 cut(s) 48, 305, 460, 630, 1459, 2369, 3007, 4073, 4109
HapII CCGG 4 cut(s) 60, 1342, 1460, 1749
HgaI GACGC 1 cut(s) 958
HhaI GCGC 4 cut(s) 218, 545, 3022, 3249
Hin1I GRCGYC 1 cut(s) 950
Hin6I GCGC 4 cut(s) 216, 543, 3020, 3247
HinP1I GCGC 4 cut(s) 216, 543, 3020, 3247
HincII GTYRAC 3 cut(s) 1071, 1846, 2136
HindII GTYRAC 3 cut(s) 1071, 1846, 2136
HpaI GTTAAC 2 cut(s) 1071, 2136
HpaII CCGG 4 cut(s) 60, 1342, 1460, 1749
HphI GGTGA 5 cut(s) 49, 602, 1972, 2069, 2138
Hpy166II GTNNAC 7 cut(s) 1071, 1427, 1846, 1963, 1981, 2136, 2549
Hpy8I GTNNAC 7 cut(s) 1071, 1427, 1846, 1963, 1981, 2136, 2549
Hpy99I CGWCG 1 cut(s) 326
HpyCH4IV ACGT 3 cut(s) 324, 397, 3204
HpyF10VI GCNNNNNNNGC 9 cut(s) 51, 224, 245, 254, 648, 2148, 3677, 3697, 4057
HpySE526I ACGT 3 cut(s) 324, 397, 3204
Hsp92I GRCGYC 1 cut(s) 950
HspAI GCGC 4 cut(s) 216, 543, 3020, 3247
Kpn2I TCCGGA 2 cut(s) 1341, 1748
KpnI GGTACC 1 cut(s) 81
Ksp22I TGATCA 2 cut(s) 1075, 2952
KspAI GTTAAC 2 cut(s) 1071, 2136
KspI CCGCGG 1 cut(s) 649
LguI GCTCTTC 1 cut(s) 499
LmnI GCTCC 5 cut(s) 100, 262, 272, 2721, 3319
Lsp1109I GCAGC 6 cut(s) 305, 2910, 3607, 3684, 3692, 3921
MabI ACCWGGT 1 cut(s) 3277
MaeI CTAG 7 cut(s) 869, 1422, 1827, 3545, 3617, 3632, 3981
MaeII ACGT 3 cut(s) 324, 397, 3204
MaeIII GTNAC 9 cut(s) 898, 958, 1562, 1604, 2276, 2479, 2801, 2965, 3010
MbiI CCGCTC 1 cut(s) 257
MflI RGATCY 4 cut(s) 1661, 1686, 3333, 3871
MhlI GDGCHC 6 cut(s) 1117, 1388, 1575, 1591, 2669, 3407
MlyI GAGTC 8 cut(s) 24, 560, 710, 890, 1298, 1525, 2405, 4111
MmeI TCCRAC 4 cut(s) 64, 2251, 2353, 3247
Mph1103I ATGCAT 1 cut(s) 3002
MroI TCCGGA 2 cut(s) 1341, 1748
MroXI GAANNNNTTC 4 cut(s) 1896, 2835, 3477, 3924
MseI TTAA 8 cut(s) 1011, 1070, 1967, 2135, 2519, 2661, 3746, 3809
MslI CAYNNNNRTG 4 cut(s) 479, 1580, 2770, 3203
MspA1I CMGCKG 2 cut(s) 648, 2599
MspCI CTTAAG 1 cut(s) 2660
MspI CCGG 4 cut(s) 60, 1342, 1460, 1749
MspR9I CCNGG 8 cut(s) 796, 1461, 1598, 1875, 3279, 3539, 3775, 4111
Mva1269I GAATGC 2 cut(s) 1179, 3198
MvaI CCWGG 7 cut(s) 796, 1598, 1875, 3279, 3539, 3775, 4111
MvnI CGCG 3 cut(s) 45, 374, 648
MwoI GCNNNNNNNGC 9 cut(s) 51, 224, 245, 254, 648, 2148, 3677, 3697, 4057
NciI CCSGG 1 cut(s) 1461
NcoI CCATGG 2 cut(s) 2177, 2253
NdeI CATATG 1 cut(s) 1559
NlaIV GGNNCC 3 cut(s) 64, 79, 2723
NmeAIII GCCGAG 2 cut(s) 281, 1435
NmuCI GTSAC 3 cut(s) 1562, 2801, 2965
NsbI TGCGCA 1 cut(s) 3248
NsiI ATGCAT 1 cut(s) 3002
NspI RCATGY 4 cut(s) 1873, 2155, 3089, 3427
NspV TTCGAA 2 cut(s) 467, 602
PaeI GCATGC 1 cut(s) 1873
PagI TCATGA 1 cut(s) 2446
PaqCI CACCTGC 2 cut(s) 287, 2088
PceI AGGCCT 1 cut(s) 3007
PciSI GCTCTTC 1 cut(s) 499
PcsI WCGNNNNNNNCGW 1 cut(s) 308
PctI GAATGC 2 cut(s) 1179, 3198
PdmI GAANNNNTTC 4 cut(s) 1896, 2835, 3477, 3924
PflMI CCANNNNNTGG 5 cut(s) 667, 945, 1314, 3538, 3620
PfoI TCCNGGA 1 cut(s) 3773
PleI GAGTC 8 cut(s) 23, 559, 709, 889, 1297, 1525, 2404, 4110
PmaCI CACGTG 1 cut(s) 3205
PmlI CACGTG 1 cut(s) 3205
PpsI GAGTC 8 cut(s) 23, 559, 709, 889, 1297, 1525, 2404, 4110
Ppu21I YACGTR 1 cut(s) 3205
PshAI GACNNNNGTC 1 cut(s) 2883
PsiI TTATAA 1 cut(s) 2589
Psp6I CCWGG 7 cut(s) 794, 1596, 1873, 3277, 3537, 3773, 4109
PspCI CACGTG 1 cut(s) 3205
PspFI CCCAGC 1 cut(s) 212
PspGI CCWGG 7 cut(s) 794, 1596, 1873, 3277, 3537, 3773, 4109
PspN4I GGNNCC 3 cut(s) 64, 79, 2723
PspPI GGNCC 8 cut(s) 629, 1200, 2540, 3275, 3741, 3845, 3969, 4071
PstI CTGCAG 1 cut(s) 1761
PstNI CAGNNNCTG 1 cut(s) 2195
PsuI RGATCY 4 cut(s) 1661, 1686, 3333, 3871
PvuII CAGCTG 1 cut(s) 2599
RsaI GTAC 8 cut(s) 79, 1637, 1797, 1944, 2032, 2501, 2506, 2835
RsaNI GTAC 8 cut(s) 78, 1636, 1796, 1943, 2031, 2500, 2505, 2834
RseI CAYNNNNRTG 4 cut(s) 479, 1580, 2770, 3203
SacII CCGCGG 1 cut(s) 649
SapI GCTCTTC 1 cut(s) 499
SaqAI TTAA 8 cut(s) 1011, 1070, 1967, 2135, 2519, 2661, 3746, 3809
Sau96I GGNCC 8 cut(s) 629, 1200, 2540, 3275, 3741, 3845, 3969, 4071
ScaI AGTACT 1 cut(s) 2835
SchI GAGTC 8 cut(s) 24, 560, 710, 890, 1298, 1525, 2405, 4111
ScrFI CCNGG 8 cut(s) 796, 1461, 1598, 1875, 3279, 3539, 3775, 4111
SduI GDGCHC 6 cut(s) 1117, 1388, 1575, 1591, 2669, 3407
SexAI ACCWGGT 1 cut(s) 3277
SfcI CTRYAG 3 cut(s) 903, 1757, 1849
Sfr303I CCGCGG 1 cut(s) 649
SfuI TTCGAA 2 cut(s) 467, 602
SgrBI CCGCGG 1 cut(s) 649
SinI GGWCC 6 cut(s) 1200, 2540, 3275, 3741, 3845, 3969
SmiMI CAYNNNNRTG 4 cut(s) 479, 1580, 2770, 3203
SmlI CTYRAG 8 cut(s) 1990, 2437, 2564, 2660, 3161, 3441, 3589, 3797
SmoI CTYRAG 8 cut(s) 1990, 2437, 2564, 2660, 3161, 3441, 3589, 3797
SphI GCATGC 1 cut(s) 1873
SseBI AGGCCT 1 cut(s) 3007
SspI AATATT 1 cut(s) 829
SspMI CTAG 7 cut(s) 869, 1422, 1827, 3545, 3617, 3632, 3981
StuI AGGCCT 1 cut(s) 3007
StyD4I CCNGG 8 cut(s) 794, 1459, 1596, 1873, 3277, 3537, 3773, 4109
StyI CCWWGG 4 cut(s) 519, 1272, 2177, 2253
TaiI ACGT 3 cut(s) 327, 400, 3207
TaqI TCGA 8 cut(s) 174, 262, 311, 336, 351, 467, 602, 1924
TatI WGTACW 3 cut(s) 1635, 2030, 2833
TauI GCSGC 6 cut(s) 48, 251, 254, 257, 308, 651
Tru1I TTAA 8 cut(s) 1011, 1070, 1967, 2135, 2519, 2661, 3746, 3809
Tru9I TTAA 8 cut(s) 1011, 1070, 1967, 2135, 2519, 2661, 3746, 3809
TscAI CASTG 9 cut(s) 505, 552, 1571, 1596, 1858, 2419, 3318, 3436, 3507
TseFI GTSAC 3 cut(s) 1562, 2801, 2965
TseI GCWGC 6 cut(s) 318, 2923, 3595, 3680, 3697, 3909
Tsp45I GTSAC 3 cut(s) 1562, 2801, 2965
TspGWI ACGGA 1 cut(s) 378
TspRI CASTG 9 cut(s) 505, 552, 1571, 1596, 1858, 2419, 3318, 3436, 3507
Van91I CCANNNNNTGG 5 cut(s) 667, 945, 1314, 3538, 3620
Vha464I CTTAAG 1 cut(s) 2660
VpaK11BI GGWCC 6 cut(s) 1200, 2540, 3275, 3741, 3845, 3969
XagI CCTNNNNNAGG 1 cut(s) 3691
XbaI TCTAGA 2 cut(s) 3544, 3631
XceI RCATGY 4 cut(s) 1873, 2155, 3089, 3427
XcmI CCANNNNNNNNNTGG 3 cut(s) 1210, 3371, 3657
XmnI GAANNNNTTC 4 cut(s) 1896, 2835, 3477, 3924
XspI CTAG 7 cut(s) 869, 1422, 1827, 3545, 3617, 3632, 3981
ZrmI AGTACT 1 cut(s) 2835
Zsp2I ATGCAT 1 cut(s) 3002
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.