RchiOBHm_Chr7g0224651

CST complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0006351
Physical Location & Seq
Forward (+)
46941165 .. 46948536
7372 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ20115

Sequence Viewer

Length: 4134 bp
ATGGAAAGTGTGAGAGTCTTGACAATCGCAGACTTACTCCAACGCGGCCGTTGCCTCACCGGAACCTCCTCTTTCGGTACCCCTTCATTTGCTCCAAAATCAAACCCCTCCATTTCTCCCATCCCCTCAAACCCTAACCCTAATGCCACCACTAAGGTTCTCCCCTCTCTCGAACGCTTTGCCCTCATCATCGGAACTGTAACTCTCCCCAGCGCCACCTCCGCCTCCGCTTCAAACGCCGCCGCTCCTCGATGCTCCTACAACACTTGCTTCCAATTCTCAGACAACTCGGCCGCCTCGATCTGCTGCGACGTTCTTGATTTCGATGCTCGGATTCTCGACAAGAGAATCAATGTTCTCGCTTGGAATTTCATTCCATTCAAACGTGGCGGTGGGTTCTTGGAGATTATCAGATGGGGGTTTCAGGATTCGATTGCTGGAATTTGGCCCTGTTCGAATATCATTTCTATGGAGTTAGGCTCTTCCACTGCTAATGCTAATGAAGACAGTTCCAAGGCTCGTCATGGTGTGTTCGGCGCACTGGAGTCGGTTAGCCCTTTCTCCATTGTTTCATGTAGAACAGGTGATAGCAATTCGAAAAGCTACAAGGCTTTGGATTCAGGCCCTCCGACAAGCCTCCGCGGCTTCTTAGTCCAATTTATGGTTTGTGAGTGTAGATCATGCAGTTCAAAGGAGTCAATCATGGTTTTGAAGGATTCAATTCAAGAAAGAGATACTCATTCTTTTACCAAACCAATGTTTGTGTATTGTTGCGGTTCTGCTTCATCCTGGCATCCGGTATTTACGAAACTTGTGGGAAATATTGTTGCTATATCAGGCCTGAAAAAGAAGTTGGTTTTTATAGGGAAGGAAGAGTCTAAAGTAATGTGTGTAACTACAGGAAGTTCTGCCTTGCATTTGAGCAGATTGTCCAAGAAATGGACGCCGAAAGTTACAGTAGGTAGAAAGGGAAATGGGGAAGTTGGTACATATAGAGGGATAGTTAAAGGTGTGTATATGCAAGGAATGGTTGTGGAATTGGACAATGAGGTGTGGCTTTTGTTAACTGATCAACTGCTTACTCCACCACATAGTCTTAGGGCGGGTGCACTCGTTTCTGTGAGAAATGCCCATTTTGTAAATCCCAGATTTTCTTGGACAAGAAGAATGCTTATACTTGGTGCTTGCTTTAGGACCAATGTAATTGTGGAATCCTTCTCTCCAATAGAAACTCGGTGTCATATAGCCTCACAATCACAGAGTTCGTTGGGGAAGTTCATTGAATCCTTAGCATTTTCTTCCAGATTATGGGTATTGCTGGTTGCCTCTTATTTCCGGAAAAAGTTTGCTGGTATCTTATCTGAGAAGGAAATTTTGGGCTCAAAACATAAGGGAGGATTAGTTGAGATGTATGCTAGTTCACAGATGCCTTCGTCAAAGCATCAAACTCGGGCGGGAGTATTCATGGAATTTTGCAAGCATGATTCCTGTGCCTGTGGTTGTGAGCCATACATAGATAATCTGACTCTGGTGATCCCCCTGTCTTTTTTCATATCTCACTGTGAGCCCGCATGGATGAGGGCACGGGACCCGGAAGGTAACAGTAGAAAATTGCATGATGACAAACAGTACAGCCGTCAATTATGTGAAGGGAGATCTTATGTTCAGTCTATTAGAAAGATCTTTTCAAGTGAAGATATAGGCATAACTTTGATTGGAAGTTTGAAGACTTCTCCATCTTCTGGAAGACTGCAGTTGGTTGATGCAACTGGTAGCATTGATGTTCTTGTACCAGACATTCCATCTACTTGGGATGCCACTAGAACAATCAAGGTAGTTGACTACAGTGTAATTATTGAAGGCGTGCCTGGGTCTGTGGATTCCGAAGGATTGCTTGAATATGATTTGTTCTCAACTCGAACTATCTTTGATTTTGTACCATTGGCAAGAAAGGTGAACTTAACAGTTTGTGTTTACTTTCGCTTGAGAAGTCCGTTGTGCAGAAATCTTTGCTTTTATCCTTGTACTGGATTGGGAGAAGATTTGAAGAGATTTGAAAGTGGAACATTTCACCTGCTATGGATTACCCACAAATTTCCTGTGCTACACAAGTTTCAAGGTGATGTGTTAACAAGCTCATTAAGCATGTTTGTTGAGGCTATAATCTTGCCATGGAATTTATCAGTTGCTGAAAACAATGGTATTGCATGTCAAACTGGGGTAGTTGGGGATGATCCAAAAAATTCCATGGAATTCTGTGCTGTTGGATGTTACTTGAAGAATGATTCTTTCAAGAGACGGAAGGTTTGTGATTCATCAAGGAAGGAACTAAGTTCTGGTTCAATGGACTGTTCCTATGAGGCTGTCGGAAAACTGAATTCTTGTTCTGAGTCTTACATTGAATCCAGTGAGGATAAAACATATAGCGACTTGAGTTGTCATGAAATATCTGGTTTGGCTATAATCAGCGAAGTTACTCGTTCAGTTATGTTGTACTGTACAAAAGCCAAATTAAATAGTGATGGCTTTTGCGGTCCAAGTGGACAGAAGATATTGCTCGAGTTTAAGTCTGACAGCTTTTATAAGTATCAGCTGTTGCAGATTGGCTGCTATTATATCACAAAGCATGATAGAGAAGATTCTTTTTGTAATCTTAAGGGCTCTGACTATTTCATTGGTAAAAAAATTCTCATCCCTCCCACAACTCATCTGTGGAGCCTTTCATTTGGTTCTGATGAGATTTGTCAAAATAACAGTTCATCAAAGTGTATTCCATTGGATGATTCTCTTATAAGTGACAAGCTTCTATCTGGATATCATAACGAAGTACTTCAGACATCCAATGAAAATTTGTCTGGAACCTCTTCAGACATGTGTCTTTGTCTGTCTGCAAGCGTATTAGGTCTTGGAGAGCTACAGCTAAAGGAACTGAAAGAAAGCCTGATCAAGCCAGTTGTGACACCAAAAGACATTCCAAAAATCTCTTCATGCATTAGGCCTGTAACGACTGCGCCTCCACTATCAACTGAATCTAACCGCATGTTTCCTGAGGGAAACCTAATATCTATGCGTGGGCATGTGGTTGCGGTTCATAGCGTTGAGGACAACTCTGTTGATCCATATTTGAACAGTCAAAACCTTCGTGATCCTCTTGAGCTGAGATTCTTGCAGAGGACAACAAGCAGTTGCATTCACGTGTTGGTGAATAATCAGATTGTAAAGCTATCTGGTTCTTTGTGCAATCATGATTTTCCAGTCGGATTTGGACCTGGTGTGGATGCAACGTTTTATCGGATTCTTGCATTGTGGGAGCAGAATAGATGGATCTTGACATCGGTATCATTTATTACAATCCATTCCATAAGTGGAGACAATGAATCATGTGGTGTCAAGTGCTCTAATCCTGCTTCATACACACCCAATGCTTCGCCTCAAGAGATTGTTCGTTCACGGTTGATATCTGAATTGGATCAAAGTTTGGACTTCAAGCCAATGCTGTTGCATTGCAGGGTTGTTGCTATCCATTTCCTGGTTCTAGAGAAGAAAAGTGGAAATGTCAATTATCAACTAAAAAATCACTTGAGGCAGCATCTTGTTGACGTCCCACTTGCTGGCTTTGTTCTAGATGACGGTTCTTCTCCATGCTGTTGTTGGGCTAATGCTGAAAGAGCAGCAACCTTGCTGAGGCTGCATGAAGAATTCCCAGAGTTAGCTTTTGAAAATAGTGGCCGGACCTTAAAGTGGGTCAGAACGGACAATAATTCATGGAGTTCCACTATCTATCATCTCGAGAGAATTTTAAATAATCATCATCGGATTGTAGTGAGGAACTATGGATCAATGTTTGATTCGTCTTATCAAGATCTCGCTGTGTCTGTGAGTTCAGATAATGCCCTCAGCAGCTATGATGAAAACCTTCTAAAGTTTATAGTCTTCAATGCTTGCTTCAGCAAATTTTGGACCATTGGTGCTAGTATGATGGATTTGGATGCTGTCAGGCGATTGAAGAGAGAGAACCTTGTGGAAATGGAGATGTTTATGCATTCCATGCAACATATATGGGCCGCAGAAGTTCAACACATTAACCATCTGGGTCAGGCCAGGAATGTTATTCAAGAACTGATAGACGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CTC1_2

Protein Analysis

1377

Amino Acids

152.83

Weight (kDa)

7.7

Isoelectric Point (pI)

44.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CTC1_2 PF15491 61 - 370 1.5e-93 CST, telomere maintenance, complex subunit CTC1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2583, 2792
AarI CACCTGC 1 cut(s) 2082
AatII GACGTC 1 cut(s) 3603
Acc36I ACCTGC 1 cut(s) 2082
Acc65I GGTACC 1 cut(s) 77
AccB1I GGYRCC 1 cut(s) 77
AccB7I CCANNNNNTGG 6 cut(s) 661, 939, 1308, 1742, 3529, 3611
AccBSI CCGCTC 1 cut(s) 245
AccII CGCG 2 cut(s) 45, 642
AccIII TCCGGA 1 cut(s) 1335
AclI AACGTT 1 cut(s) 3284
AclWI GGATC 7 cut(s) 1528, 2228, 3110, 3140, 3332, 3477, 3844
AcoI YGGCCR 3 cut(s) 46, 291, 3727
AcuI CTGAAG 3 cut(s) 2816, 2850, 3931
AcvI CACGTG 1 cut(s) 3196
AcyI GRCGYC 2 cut(s) 944, 3600
AfaI GTAC 9 cut(s) 79, 988, 1631, 1791, 1938, 2026, 2495, 2500, 2829
AfiI CCNNNNNNNGG 9 cut(s) 661, 939, 1308, 1742, 2027, 3529, 3611, 3684, 3741
AflII CTTAAG 1 cut(s) 2654
AflIII ACRYGT 2 cut(s) 2871, 3195
AjnI CCWGG 5 cut(s) 788, 1867, 3268, 3528, 4100
AjuI GAANNNNNNNTTGG 6 cut(s) 836, 868, 1358, 1390, 2968, 3000
Alw21I GWGCWC 2 cut(s) 1111, 3398
Alw26I GTCTC 2 cut(s) 2290, 3363
Alw44I GTGCAC 1 cut(s) 1107
AlwI GGATC 7 cut(s) 1528, 2228, 3110, 3140, 3332, 3477, 3844
AlwNI CAGNNNCTG 1 cut(s) 2189
Ama87I CYCGRG 3 cut(s) 1449, 2558, 3788
Aor13HI TCCGGA 1 cut(s) 1335
AoxI GGCC 9 cut(s) 46, 291, 446, 622, 838, 2996, 3727, 4062, 4098
ApaLI GTGCAC 1 cut(s) 1107
ApeKI GCWGC 6 cut(s) 306, 2607, 3586, 3671, 3688, 3900
ArsI GACNNNNNNTTYG 2 cut(s) 2289, 2321
Asp700I GAANNNNTTC 3 cut(s) 2829, 2863, 3915
Asp718I GGTACC 1 cut(s) 77
AspLEI GCGC 3 cut(s) 215, 539, 3013
AspS9I GGNCC 9 cut(s) 447, 623, 1194, 1588, 2534, 3266, 3732, 3960, 4062
AsuC2I CCSGG 1 cut(s) 1592
AsuHPI GGTGA 7 cut(s) 49, 596, 1543, 1966, 2063, 2132, 3214
AsuII TTCGAA 2 cut(s) 455, 596
AvaI CYCGRG 3 cut(s) 1449, 2558, 3788
AvaII GGWCC 6 cut(s) 1194, 1588, 2534, 3266, 3732, 3960
AxyI CCTNAGG 1 cut(s) 3048
BaeGI GKGCMC 2 cut(s) 1111, 1585
BaeI ACNNNNGTAYC 2 cut(s) 3321, 3354
BanI GGYRCC 1 cut(s) 77
BanII GRGCYC 3 cut(s) 1382, 1569, 2663
BbrPI CACGTG 1 cut(s) 3196
BbsI GAAGAC 4 cut(s) 510, 1733, 1753, 3925
Bbv12I GWGCWC 2 cut(s) 1111, 3398
BbvCI CCTCAGC 2 cut(s) 3683, 3896
BbvI GCAGC 6 cut(s) 293, 2594, 3598, 3675, 3683, 3912
BccI CCATC 8 cut(s) 128, 408, 1744, 1810, 2516, 3315, 3973, 4095
BceAI ACGGC 2 cut(s) 33, 1620
BcgI CGANNNNNNTGC 6 cut(s) 161, 195, 528, 562, 1430, 1464
BciT130I CCWGG 5 cut(s) 790, 1869, 3270, 3530, 4102
BclI TGATCA 2 cut(s) 1069, 2943
BcnI CCSGG 1 cut(s) 1592
BcoDI GTCTC 2 cut(s) 2290, 3363
BfaI CTAG 5 cut(s) 1416, 1821, 3536, 3623, 3972
BfmI CTRYAG 4 cut(s) 897, 1751, 1843, 2915
BfoI RGCGCY 1 cut(s) 216
BfrI CTTAAG 1 cut(s) 2654
BfuAI ACCTGC 1 cut(s) 2082
BglI GCCNNNNNGGC 1 cut(s) 642
BglII AGATCT 3 cut(s) 1655, 1680, 3862
BmcAI AGTACT 1 cut(s) 2829
Bme1390I CCNGG 6 cut(s) 790, 1592, 1869, 3270, 3530, 4102
Bme18I GGWCC 6 cut(s) 1194, 1588, 2534, 3266, 3732, 3960
BmeT110I CYCGRG 3 cut(s) 1449, 2558, 3788
BmgT120I GGNCC 9 cut(s) 447, 623, 1194, 1588, 2534, 3266, 3732, 3960, 4062
BmiI GGNNCC 6 cut(s) 64, 79, 1589, 1590, 2717, 2860
BmrFI CCNGG 6 cut(s) 790, 1592, 1869, 3270, 3530, 4102
BmrI ACTGGG 1 cut(s) 2226
BmuI ACTGGG 1 cut(s) 2226
BoxI GACNNNNGTC 1 cut(s) 2874
BpiI GAAGAC 4 cut(s) 510, 1733, 1753, 3925
BplI GAGNNNNNCTC 4 cut(s) 464, 496, 3092, 3124
BpmI CTGGAG 1 cut(s) 563
Bpu10I CCTNAGC 3 cut(s) 1288, 3683, 3896
Bpu14I TTCGAA 2 cut(s) 455, 596
BpuEI CTTGAG 5 cut(s) 2005, 2452, 3173, 3417, 3601
BpuMI CCSGG 1 cut(s) 1592
BsaAI YACGTR 1 cut(s) 3196
BsaHI GRCGYC 2 cut(s) 944, 3600
BsaJI CCNNGG 5 cut(s) 513, 640, 1868, 2171, 2247
BsaWI WCCGGW 3 cut(s) 59, 796, 1335
BsaXI ACNNNNNCTCC 4 cut(s) 1386, 1416, 2998, 3028
Bsc4I CCNNNNNNNGG 9 cut(s) 661, 939, 1308, 1742, 2027, 3529, 3611, 3684, 3741
Bse1I ACTGG 7 cut(s) 546, 1774, 2032, 2221, 2406, 2951, 3254
Bse21I CCTNAGG 1 cut(s) 3048
Bse3DI GCAATG 1 cut(s) 3502
BseAI TCCGGA 1 cut(s) 1335
BseBI CCWGG 5 cut(s) 790, 1869, 3270, 3530, 4102
BseDI CCNNGG 5 cut(s) 513, 640, 1868, 2171, 2247
BseLI CCNNNNNNNGG 9 cut(s) 661, 939, 1308, 1742, 2027, 3529, 3611, 3684, 3741
BseMI GCAATG 1 cut(s) 3502
BseMII CTCAG 7 cut(s) 294, 1353, 2379, 3039, 3149, 3674, 3910
BseNI ACTGG 7 cut(s) 546, 1774, 2032, 2221, 2406, 2951, 3254
BseRI GAGGAG 2 cut(s) 58, 237
BseSI GKGCMC 2 cut(s) 1111, 1585
BseX3I CGGCCG 2 cut(s) 46, 291
BseXI GCAGC 6 cut(s) 293, 2594, 3598, 3675, 3683, 3912
BseYI CCCAGC 1 cut(s) 209
BsgI GTGCAG 1 cut(s) 2020
Bsh1236I CGCG 2 cut(s) 45, 642
Bsh1285I CGRYCG 2 cut(s) 49, 294
BshFI GGCC 9 cut(s) 48, 293, 448, 624, 840, 2998, 3729, 4064, 4100
BshNI GGYRCC 1 cut(s) 77
BsiEI CGRYCG 2 cut(s) 49, 294
BsiHKAI GWGCWC 2 cut(s) 1111, 3398
BsiHKCI CYCGRG 3 cut(s) 1449, 2558, 3788
BsiSI CCGG 5 cut(s) 60, 797, 1336, 1592, 3730
BslFI GGGAC 2 cut(s) 1601, 3587
BslI CCNNNNNNNGG 9 cut(s) 661, 939, 1308, 1742, 2027, 3529, 3611, 3684, 3741
BsmAI GTCTC 2 cut(s) 2290, 3363
BsmBI CGTCTC 1 cut(s) 2290
BsmFI GGGAC 2 cut(s) 1601, 3587
BsmI GAATGC 3 cut(s) 1173, 3189, 4042
BsnI GGCC 9 cut(s) 48, 293, 448, 624, 840, 2998, 3729, 4064, 4100
BsoBI CYCGRG 3 cut(s) 1449, 2558, 3788
Bsp119I TTCGAA 2 cut(s) 455, 596
Bsp1286I GDGCHC 6 cut(s) 1111, 1382, 1569, 1585, 2663, 3398
Bsp13I TCCGGA 1 cut(s) 1335
Bsp1407I TGTACA 1 cut(s) 2498
Bsp19I CCATGG 2 cut(s) 2171, 2247
BspANI GGCC 9 cut(s) 48, 293, 448, 624, 840, 2998, 3729, 4064, 4100
BspCNI CTCAG 7 cut(s) 293, 1354, 2380, 3040, 3150, 3675, 3909
BspEI TCCGGA 1 cut(s) 1335
BspFNI CGCG 2 cut(s) 45, 642
BspHI TCATGA 2 cut(s) 2440, 3244
BspLI GGNNCC 6 cut(s) 64, 79, 1589, 1590, 2717, 2860
BspMAI CTGCAG 1 cut(s) 1755
BspMI ACCTGC 1 cut(s) 2082
BspPI GGATC 7 cut(s) 1528, 2228, 3110, 3140, 3332, 3477, 3844
BspQI GCTCTTC 1 cut(s) 487
BspT104I TTCGAA 2 cut(s) 455, 596
BspT107I GGYRCC 1 cut(s) 77
BspTI CTTAAG 1 cut(s) 2654
BsrBI CCGCTC 1 cut(s) 245
BsrDI GCAATG 1 cut(s) 3502
BsrGI TGTACA 1 cut(s) 2498
BsrI ACTGG 7 cut(s) 546, 1774, 2032, 2221, 2406, 2951, 3254
BssECI CCNNGG 5 cut(s) 513, 640, 1868, 2171, 2247
BssNI GRCGYC 2 cut(s) 944, 3600
BssT1I CCWWGG 3 cut(s) 513, 2171, 2247
Bst2UI CCWGG 5 cut(s) 790, 1869, 3270, 3530, 4102
Bst6I CTCTTC 6 cut(s) 487, 867, 2042, 2869, 2989, 4001
BstACI GRCGYC 2 cut(s) 944, 3600
BstAFI CTTAAG 1 cut(s) 2654
BstAPI GCANNNNNTGC 1 cut(s) 4048
BstAUI TGTACA 1 cut(s) 2498
BstBAI YACGTR 1 cut(s) 3196
BstBI TTCGAA 2 cut(s) 455, 596
BstC8I GCNNGC 7 cut(s) 1186, 1478, 1569, 1865, 2893, 3613, 3943
BstDSI CCRYGG 3 cut(s) 640, 2171, 2247
BstENI CCTNNNNNAGG 1 cut(s) 3682
BstFNI CGCG 2 cut(s) 45, 642
BstH2I RGCGCY 1 cut(s) 216
BstHHI GCGC 3 cut(s) 215, 539, 3013
BstMAI GTCTC 2 cut(s) 2290, 3363
BstMCI CGRYCG 2 cut(s) 49, 294
BstMWI GCNNNNNNNGC 8 cut(s) 51, 221, 236, 642, 2142, 3668, 3688, 4048
BstNI CCWGG 5 cut(s) 790, 1869, 3270, 3530, 4102
BstNSI RCATGY 5 cut(s) 2149, 2211, 2875, 3043, 3080
BstPAI GACNNNNGTC 1 cut(s) 2874
BstSCI CCNGG 6 cut(s) 788, 1590, 1867, 3268, 3528, 4100
BstSFI CTRYAG 4 cut(s) 897, 1751, 1843, 2915
BstSLI GKGCMC 2 cut(s) 1111, 1585
BstUI CGCG 2 cut(s) 45, 642
BstV1I GCAGC 6 cut(s) 293, 2594, 3598, 3675, 3683, 3912
BstV2I GAAGAC 4 cut(s) 510, 1733, 1753, 3925
BstX2I RGATCY 4 cut(s) 1655, 1680, 3324, 3862
BstXI CCANNNNNNTGG 1 cut(s) 1809
BstYI RGATCY 4 cut(s) 1655, 1680, 3324, 3862
BstZI CGGCCG 2 cut(s) 46, 291
Bsu36I CCTNAGG 1 cut(s) 3048
BsuRI GGCC 9 cut(s) 48, 293, 448, 624, 840, 2998, 3729, 4064, 4100
BtgI CCRYGG 3 cut(s) 640, 2171, 2247
BtsI GCAGTG 1 cut(s) 486
BtsIMutI CAGTG 5 cut(s) 486, 539, 1558, 1852, 2413
BveI ACCTGC 1 cut(s) 2082
Cac8I GCNNGC 7 cut(s) 1186, 1478, 1569, 1865, 2893, 3613, 3943
CaiI CAGNNNCTG 1 cut(s) 2189
CciI TCATGA 2 cut(s) 2440, 3244
CfoI GCGC 3 cut(s) 215, 539, 3013
Cfr13I GGNCC 9 cut(s) 447, 623, 1194, 1588, 2534, 3266, 3732, 3960, 4062
Cfr42I CCGCGG 1 cut(s) 643
CseI GACGC 1 cut(s) 952
CsiI ACCWGGT 1 cut(s) 3268
Csp6I GTAC 9 cut(s) 78, 987, 1630, 1790, 1937, 2025, 2494, 2499, 2828
CviQI GTAC 9 cut(s) 78, 987, 1630, 1790, 1937, 2025, 2494, 2499, 2828
DraI TTTAAA 1 cut(s) 3801
EaeI YGGCCR 3 cut(s) 46, 291, 3727
EagI CGGCCG 2 cut(s) 46, 291
Eam1104I CTCTTC 6 cut(s) 487, 867, 2042, 2869, 2989, 4001
EarI CTCTTC 6 cut(s) 487, 867, 2042, 2869, 2989, 4001
EciI GGCGGA 1 cut(s) 211
EclXI CGGCCG 2 cut(s) 46, 291
Eco130I CCWWGG 3 cut(s) 513, 2171, 2247
Eco147I AGGCCT 2 cut(s) 840, 2998
Eco24I GRGCYC 3 cut(s) 1382, 1569, 2663
Eco32I GATATC 2 cut(s) 2816, 3459
Eco47I GGWCC 6 cut(s) 1194, 1588, 2534, 3266, 3732, 3960
Eco52I CGGCCG 2 cut(s) 46, 291
Eco57I CTGAAG 3 cut(s) 2816, 2850, 3931
Eco72I CACGTG 1 cut(s) 3196
Eco81I CCTNAGG 1 cut(s) 3048
Eco88I CYCGRG 3 cut(s) 1449, 2558, 3788
EcoNI CCTNNNNNAGG 1 cut(s) 3682
EcoO109I RGGNCCY 2 cut(s) 623, 1588
EcoRI GAATTC 3 cut(s) 2252, 2377, 3698
EcoRII CCWGG 5 cut(s) 788, 1867, 3268, 3528, 4100
EcoRV GATATC 2 cut(s) 2816, 3459
EcoT14I CCWWGG 3 cut(s) 513, 2171, 2247
EcoT22I ATGCAT 2 cut(s) 2993, 4044
EcoT38I GRGCYC 3 cut(s) 1382, 1569, 2663
ErhI CCWWGG 3 cut(s) 513, 2171, 2247
Esp3I CGTCTC 1 cut(s) 2290
FalI AAGNNNNNCTT 4 cut(s) 1878, 1910, 1943, 1975
FaqI GGGAC 2 cut(s) 1601, 3587
FauI CCCGC 3 cut(s) 1096, 1447, 1576
FbaI TGATCA 2 cut(s) 1069, 2943
FriOI GRGCYC 3 cut(s) 1382, 1569, 2663
FspBI CTAG 5 cut(s) 1416, 1821, 3536, 3623, 3972
GlaI GCGC 3 cut(s) 214, 538, 3012
GsaI CCCAGC 1 cut(s) 213
GsuI CTGGAG 1 cut(s) 563
HaeII RGCGCY 1 cut(s) 216
HaeIII GGCC 9 cut(s) 48, 293, 448, 624, 840, 2998, 3729, 4064, 4100
HapII CCGG 5 cut(s) 60, 797, 1336, 1592, 3730
HgaI GACGC 1 cut(s) 952
HhaI GCGC 3 cut(s) 215, 539, 3013
Hin1I GRCGYC 2 cut(s) 944, 3600
Hin6I GCGC 3 cut(s) 213, 537, 3011
HinP1I GCGC 3 cut(s) 213, 537, 3011
HincII GTYRAC 4 cut(s) 1065, 1840, 2130, 3598
HindII GTYRAC 4 cut(s) 1065, 1840, 2130, 3598
HindIII AAGCTT 1 cut(s) 2801
HpaI GTTAAC 2 cut(s) 1065, 2130
HpaII CCGG 5 cut(s) 60, 797, 1336, 1592, 3730
HphI GGTGA 7 cut(s) 49, 596, 1543, 1966, 2063, 2132, 3214
Hpy99I CGWCG 1 cut(s) 314
HpyCH4IV ACGT 5 cut(s) 312, 385, 3195, 3284, 3600
HpyF10VI GCNNNNNNNGC 8 cut(s) 51, 221, 236, 642, 2142, 3668, 3688, 4048
HpySE526I ACGT 5 cut(s) 312, 385, 3195, 3284, 3600
Hsp92I GRCGYC 2 cut(s) 944, 3600
HspAI GCGC 3 cut(s) 213, 537, 3011
KflI GGGWCCC 1 cut(s) 1588
Kpn2I TCCGGA 1 cut(s) 1335
KpnI GGTACC 1 cut(s) 81
Ksp22I TGATCA 2 cut(s) 1069, 2943
KspAI GTTAAC 2 cut(s) 1065, 2130
KspI CCGCGG 1 cut(s) 643
LguI GCTCTTC 1 cut(s) 487
LmnI GCTCC 5 cut(s) 97, 250, 260, 2715, 3310
Lsp1109I GCAGC 6 cut(s) 293, 2594, 3598, 3675, 3683, 3912
MabI ACCWGGT 1 cut(s) 3268
MaeI CTAG 5 cut(s) 1416, 1821, 3536, 3623, 3972
MaeII ACGT 5 cut(s) 312, 385, 3195, 3284, 3600
MaeIII GTNAC 9 cut(s) 199, 892, 952, 1598, 2270, 2473, 2795, 2956, 3001
MbiI CCGCTC 1 cut(s) 245
MflI RGATCY 4 cut(s) 1655, 1680, 3324, 3862
MhlI GDGCHC 6 cut(s) 1111, 1382, 1569, 1585, 2663, 3398
MlyI GAGTC 6 cut(s) 24, 554, 704, 884, 1519, 2399
MmeI TCCRAC 5 cut(s) 64, 653, 2245, 2347, 3238
Mph1103I ATGCAT 2 cut(s) 2993, 4044
MroI TCCGGA 1 cut(s) 1335
MroXI GAANNNNTTC 3 cut(s) 2829, 2863, 3915
MslI CAYNNNNRTG 3 cut(s) 467, 2764, 3194
MspA1I CMGCKG 2 cut(s) 642, 2593
MspCI CTTAAG 1 cut(s) 2654
MspI CCGG 5 cut(s) 60, 797, 1336, 1592, 3730
MspR9I CCNGG 6 cut(s) 790, 1592, 1869, 3270, 3530, 4102
Mva1269I GAATGC 3 cut(s) 1173, 3189, 4042
MvaI CCWGG 5 cut(s) 790, 1869, 3270, 3530, 4102
MvnI CGCG 2 cut(s) 45, 642
MwoI GCNNNNNNNGC 8 cut(s) 51, 221, 236, 642, 2142, 3668, 3688, 4048
NciI CCSGG 1 cut(s) 1592
NcoI CCATGG 2 cut(s) 2171, 2247
NlaIV GGNNCC 6 cut(s) 64, 79, 1589, 1590, 2717, 2860
NmeAIII GCCGAG 1 cut(s) 269
NmuCI GTSAC 2 cut(s) 2795, 2956
NsiI ATGCAT 2 cut(s) 2993, 4044
NspI RCATGY 5 cut(s) 2149, 2211, 2875, 3043, 3080
NspV TTCGAA 2 cut(s) 455, 596
PaeR7I CTCGAG 2 cut(s) 2558, 3788
PagI TCATGA 2 cut(s) 2440, 3244
PaqCI CACCTGC 1 cut(s) 2082
PceI AGGCCT 2 cut(s) 840, 2998
PciI ACATGT 1 cut(s) 2871
PciSI GCTCTTC 1 cut(s) 487
PcsI WCGNNNNNNNCGW 1 cut(s) 296
PctI GAATGC 3 cut(s) 1173, 3189, 4042
PdmI GAANNNNTTC 3 cut(s) 2829, 2863, 3915
PflMI CCANNNNNTGG 6 cut(s) 661, 939, 1308, 1742, 3529, 3611
PleI GAGTC 6 cut(s) 23, 553, 703, 883, 1519, 2398
PmaCI CACGTG 1 cut(s) 3196
PmlI CACGTG 1 cut(s) 3196
PpsI GAGTC 6 cut(s) 23, 553, 703, 883, 1519, 2398
Ppu21I YACGTR 1 cut(s) 3196
PpuMI RGGWCCY 1 cut(s) 1588
PscI ACATGT 1 cut(s) 2871
PshAI GACNNNNGTC 1 cut(s) 2874
PsiI TTATAA 2 cut(s) 2583, 2792
Psp1406I AACGTT 1 cut(s) 3284
Psp5II RGGWCCY 1 cut(s) 1588
Psp6I CCWGG 5 cut(s) 788, 1867, 3268, 3528, 4100
PspCI CACGTG 1 cut(s) 3196
PspFI CCCAGC 1 cut(s) 209
PspGI CCWGG 5 cut(s) 788, 1867, 3268, 3528, 4100
PspN4I GGNNCC 6 cut(s) 64, 79, 1589, 1590, 2717, 2860
PspPI GGNCC 9 cut(s) 447, 623, 1194, 1588, 2534, 3266, 3732, 3960, 4062
PspPPI RGGWCCY 1 cut(s) 1588
PspXI VCTCGAGB 1 cut(s) 2558
PstI CTGCAG 1 cut(s) 1755
PstNI CAGNNNCTG 1 cut(s) 2189
PsuI RGATCY 4 cut(s) 1655, 1680, 3324, 3862
PvuII CAGCTG 1 cut(s) 2593
RsaI GTAC 9 cut(s) 79, 988, 1631, 1791, 1938, 2026, 2495, 2500, 2829
RsaNI GTAC 9 cut(s) 78, 987, 1630, 1790, 1937, 2025, 2494, 2499, 2828
RseI CAYNNNNRTG 3 cut(s) 467, 2764, 3194
SacII CCGCGG 1 cut(s) 643
SapI GCTCTTC 1 cut(s) 487
Sau96I GGNCC 9 cut(s) 447, 623, 1194, 1588, 2534, 3266, 3732, 3960, 4062
ScaI AGTACT 1 cut(s) 2829
SchI GAGTC 6 cut(s) 24, 554, 704, 884, 1519, 2399
ScrFI CCNGG 6 cut(s) 790, 1592, 1869, 3270, 3530, 4102
SduI GDGCHC 6 cut(s) 1111, 1382, 1569, 1585, 2663, 3398
SexAI ACCWGGT 1 cut(s) 3268
SfcI CTRYAG 4 cut(s) 897, 1751, 1843, 2915
Sfr274I CTCGAG 2 cut(s) 2558, 3788
Sfr303I CCGCGG 1 cut(s) 643
SfuI TTCGAA 2 cut(s) 455, 596
SgrBI CCGCGG 1 cut(s) 643
SinI GGWCC 6 cut(s) 1194, 1588, 2534, 3266, 3732, 3960
SlaI CTCGAG 2 cut(s) 2558, 3788
SmiMI CAYNNNNRTG 3 cut(s) 467, 2764, 3194
SmlI CTYRAG 8 cut(s) 1984, 2431, 2558, 2654, 3152, 3432, 3580, 3788
SmoI CTYRAG 8 cut(s) 1984, 2431, 2558, 2654, 3152, 3432, 3580, 3788
SseBI AGGCCT 2 cut(s) 840, 2998
SspI AATATT 1 cut(s) 823
SspMI CTAG 5 cut(s) 1416, 1821, 3536, 3623, 3972
StuI AGGCCT 2 cut(s) 840, 2998
StyD4I CCNGG 6 cut(s) 788, 1590, 1867, 3268, 3528, 4100
StyI CCWWGG 3 cut(s) 513, 2171, 2247
TaiI ACGT 5 cut(s) 315, 388, 3198, 3287, 3603
TatI WGTACW 5 cut(s) 1629, 2024, 2493, 2498, 2827
TauI GCSGC 6 cut(s) 48, 242, 245, 296, 645, 4067
TscAI CASTG 5 cut(s) 493, 546, 1565, 1852, 2413
TseFI GTSAC 2 cut(s) 2795, 2956
TseI GCWGC 6 cut(s) 306, 2607, 3586, 3671, 3688, 3900
Tsp45I GTSAC 2 cut(s) 2795, 2956
TspGWI ACGGA 3 cut(s) 1983, 2314, 3767
TspRI CASTG 5 cut(s) 493, 546, 1565, 1852, 2413
Van91I CCANNNNNTGG 6 cut(s) 661, 939, 1308, 1742, 3529, 3611
Vha464I CTTAAG 1 cut(s) 2654
VneI GTGCAC 1 cut(s) 1107
VpaK11BI GGWCC 6 cut(s) 1194, 1588, 2534, 3266, 3732, 3960
XagI CCTNNNNNAGG 1 cut(s) 3682
XbaI TCTAGA 2 cut(s) 3535, 3622
XceI RCATGY 5 cut(s) 2149, 2211, 2875, 3043, 3080
XcmI CCANNNNNNNNNTGG 3 cut(s) 1204, 3362, 3648
XhoI CTCGAG 2 cut(s) 2558, 3788
XmnI GAANNNNTTC 3 cut(s) 2829, 2863, 3915
XspI CTAG 5 cut(s) 1416, 1821, 3536, 3623, 3972
ZraI GACGTC 1 cut(s) 3601
ZrmI AGTACT 1 cut(s) 2829
Zsp2I ATGCAT 2 cut(s) 2993, 4044
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.