RLG00000001867

CST complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0006351
Physical Location & Seq
Reverse (-)
22253079 .. 22260036
6958 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001867

Sequence Viewer

Length: 4122 bp
ATGGAAAATGTGAGAGTCTTGACAATCGCAGAGTTACTCCAACGCGGCCGTTGCCTCACCGGAACCTCCTCTTTCGGTACCCCCTCCTTTGCTCCAAAATCAAACCCCTCCGTTTCTCCCATCCCCTCAAACCCTAACCCTAATTCCACCACTAAGGTTCTCCCCTCTCTCGACCGCTTTGCCCTCATCATCGGAACTATAACTCTCCCCAGCGCCACCTCTACCTCCGCTTCAAGCGCCGCCGCTCCTCGATGCTCCTACAACACTTGCTTCCAGTTCTCAGACAACTCGGCCGCCTCGATCTGCTGTGACGTTCTTGATTTCGATGCTCGGATTCTCGACAAGAGAATCAATGTTCTTGCTTGGAATTTCATTCCGTTCAAACGTGGCGGTGGGTTCTTGGAGATTATCAGATGGGGGTTTCAGGATTCAATAGGTGGAATTTGGCCGTGTTCGAATATCATTTCTATGGAGTTAGGCTCTTCAACTGCTAATGAAGACAGTTCGAAGGCTCGTCATGGTGTGTTCGGCGCACTGGAGTCGGTTAGCCCTTTGTCCATTGTTTCATGTAGAACAGGTGATAGCAATTCGAAAAGCAACAAGGCTTTGGATTCAGGCCCTCCGACAAGCCTCCGTGGCTTCTTAGTCCAATTTATGGTTTGTGAGTGTAGATCATGTGGTTCAAAGGAGTCAATCATGGTTTTGAAGGATTCAATTCAAGAAAGAGATGCTCATTCTTTTACCAAACCGATGTTTGTATATTGTTGCGGTTCTGCTTCATCCTGGCATCCTGTATTTACGAAACTTGTGGGAAATATTGTTGCTATATCAGGCCTGAAAAAGAAGTTGGTATTTATAGGGAAGGAAGAGTCTAAAGTAATGTGTGTAACTACAGGAAGTTCTGCCTTGCATTTGAGCAGATTGTCCAAGAAATGGACGCCGAAAGTTACAATAGGTAGAAAGGGAAATGGGGAAGTTGGTTCATATAGAGGGATAGTTAAAGGTGTGTATATGCAAGGAATGGTTGTGGAATTGGACAATGAGGTGTGGCTTTTGTTAACTGATCAACTGCTTACTCCACCACATAGTCTTAGGGCAGGTGCTCTCGTTTCTGTCAGAAATGCCCATTTTGTAAATCCCAGATTTTCTTGGACAAGAAGAATGCTTATACTTGGTGCTTGCTTTAGGACCAGTGTAATTGTGGATTCCTTCTCTCCGATAGAAACTCGGTGTCATATAGCCTCACAATCACAGAGCTCCTTGGGGAAGTTCATCGAGTCCTTAGCATATTCTTCCAGATTATGGGTATTGCTGGTTGCCTCGTATTTCCGGAAAAAGTTTGCTGGTATCTTATCTGAGAAGGAAATTTTGGGCTCAAAACATAAGGGAGGATTAGTTGAGATGTATGCTAGTTCACAGATGCCTTCGTCAAAGCATCAAACTCGGCCGGGAGTATTCATGGAATTTTGCAAGCATGATTCCCGTGCCTGTGGTTGTGAGCCATACATAGATAATCTGACTCTGGTGATCCCCTTGTCTTTTTTCATATGTCACTGTGAGCCCACATGGATGAGGGCACTGAACCTGGAAGGTAACAGTGGAAAATTGCATGATGACAAACACTACAGCCGTCAATTATGTGAAGGGAGATCTTATGTTCAGTCTATTAGAAAGATCTTTTTAAGTGAAGATATAGGCATAACTTTGATTGGAAGTTTGAAGACTTCTCCATCTTCCGGAAGACTGCAGTTGGTTGATGCAACTGGTAGCATTGATGTTCTTGTACCAGACCTTCCATCAACTTGGGATGCCACTAGAATAATCAAGGTAGTTGATTACAGTGTAATTATTGAAGGCATGCCTGGGTCTGTGGATTCCGAAGGATTGGTTGAATATGATTTGCTATCAACTCGAACTATCTTTGATTTTGTACCATTGACAAGAAAGGTGAACTTAACAGTTTGTGTTTACTTTCGCTTGAGAAGTCCGTTGTGCAGAAATCTTTGCTTTTATCCTTGTACTGGATTGGAAGAAGATTTGAAGAGATTTGAAAGTGGAACATATCACCTGCTATGGATTACCCACAAATTTCCTGTGCTACACAAGTTTCAAGGTGATGTGTTAACAAGTTCATCAAGCATGTTTGTTGAGGCTATAATCTTGCCATGGAATTTATCAGTTGCTGAAAACAATGGTATTGTGTGTCAAACTGGGGTAGTTGGGGATGAACGAAAAAATTCCGTGGAGTTTTGTGCTGTTGGATGTTACTTGAAGAATGATTCTTTCAAGAGACGGAAGGTTTGTGATTCATCAAGGAAGGAACTAATGGACTGTTCCTGTGAGGCCGTCGGAAAACTGAATTCTGGTTCTGAGTCCTACATCGAATCCAGTGAGGATAAAACATATAGCGACTTGAGTTGTCCTGAAATATCTGGTTTGGCTATAATTAGTGGAGTTACTCGTTCAGTTATCTTGTACCGTACAAAAGCCAAATTAAACAGTGGTGGCTTTTGCAGTCCAAGTGGACAGAATAAGATATTGCTCGAGTTTAAGTCTGACAGCTCTTATAAATATCAGCTGTTGCAGATTGGCTGTTATTATATCACAAAGCATGATAGAGAAGATTCTTTTTGTAATCTTAAGGGCTCTGACTCTTTCATTAGTAAAAAAATTCTCATCCCTTCCACAACTGATCTGTGGAGCCTTTCATTTGGTTCTGATGAGGTTTGGCAAAATAACAGTTCATCAAAGTGTATTCCATTGGATGATTCTCTTAGAAGTGACGAGCTTCTATCTGGATTTCATAACGAAGTACTTCTTCAGACGTCCAATGAAAATTTGTCTGAAACCTCTTCAGACATGTGTCTTTGCCTGTCTGCAAGCATATTAGGTCTTGGAGAGCCACACCTAAAGGAACTGAAAGAAAGCCTGATCAAGCCAGTTGTGACACCTAAAGACATTCCAAAAATCTCTTCATGCATTAGGCCTGTAATGACTGCGCCTCCACTATCAACTGAATCTAACCGCATGTTTCCTGAGGGAAACCTAATATCTATGCATGGGCATGTGGTTGCAGTTCATAGCATTGAGGACAACCCTGTTGATCCATACTTGAACCGTCAAAACCTTCGTGATCCTCTTGAGCTGAGATTCTTGCAGAGGACAACAAGCAGTTGCATTCACGTGTTGGTGGATAATCAGATTGTAAAGCTATCTGGTTCTTTGCGCAAACATGATTTTCCAGTCGGATTTGGACCCGGTGTGGATGCAACGTTTTATCGGATTCTTGCATTGTGGGAGCAGAATAGATGGATCTTGACATCGGTATCATTTATTACAATCCATTCCATAAGCGGAGACAATGAATCATGTGGTGTCAAGTGCTCTAATCCTGCTTCATACATGCCCAATGCTTTGCCTCAAGAGATTGTTCGTTCAGGGTTGATATCTGAATTGGTTCAAAGTTTGGACTTCAAGCCAATGCTGTTGCATTGCAGGGTTGTTGCTATCCATTTCCTGGTTCTAGAGAAGAAAAGTAGAAATGTCAATTATCAACTAAAAAATCACTTGAGGCAGCATCTTGTTGACATCCCACTTGCTGGCTTTGTTCTAGATGATGGTTCTTCTCCATGCTGTTGTTGGGCTAATGCTGAAAGAGCAGCAACCTTGCTGAGGCTGCATGAAGAATTCCCACAGTTAGCTTTTGAAAATAGTGGCTGGACCTTAAAGTGGGTCAGAACGAACAATAATTCATGGAGTTCCACTATCTATCATCTCGAGAGAATTTTAAATAATCATCATCGGATCGTAGTGAGGAACTATGGATCAATGTTTGATTCGTCCTATCAAGATCTCGCTGTGTCTGTTAGTTCAGATAATGCCCTCAGCAGCTATGATGAAAACCTTCTAAAGTTTATAGTCTTCAATGCTTGCTTCAGCAAATTTTGGACCATTGGTGCTAGTATGATGGATTTGGATGCTGTCAGGCGATTGAAGACAGAGAACCTTGTGGAAACGGAGATGTCTATGCAATCCATGCAACATATATGGGCCACAGAAGTTCAACACATTAACCATCTGAGTCAGGCCAGGAATGTTATTCAAGAACTGATGGACGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CTC1_2

Protein Analysis

1374

Amino Acids

152.62

Weight (kDa)

7.93

Isoelectric Point (pI)

47.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CTC1_2 PF15491 61 - 368 1.2e-92 CST, telomere maintenance, complex subunit CTC1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2568
AarI CACCTGC 2 cut(s) 1088, 2076
AatII GACGTC 1 cut(s) 2828
Acc16I TGCGCA 1 cut(s) 3227
Acc36I ACCTGC 2 cut(s) 1088, 2076
Acc65I GGTACC 1 cut(s) 77
AccB1I GGYRCC 1 cut(s) 77
AccB7I CCANNNNNTGG 5 cut(s) 655, 933, 1302, 3517, 3599
AccBSI CCGCTC 1 cut(s) 245
AccII CGCG 1 cut(s) 45
AccIII TCCGGA 2 cut(s) 1329, 1736
AclI AACGTT 1 cut(s) 3272
AclWI GGATC 6 cut(s) 1522, 3098, 3128, 3320, 3812, 3832
AcoI YGGCCR 4 cut(s) 46, 291, 446, 1445
AcuI CTGAAG 3 cut(s) 2804, 2838, 3919
AcvI CACGTG 1 cut(s) 3184
AcyI GRCGYC 2 cut(s) 938, 2825
AfaI GTAC 7 cut(s) 79, 1785, 1932, 2020, 2477, 2482, 2814
AfiI CCNNNNNNNGG 9 cut(s) 655, 933, 1302, 1736, 2021, 3517, 3599, 3672, 3729
AflII CTTAAG 1 cut(s) 2639
AflIII ACRYGT 2 cut(s) 2859, 3183
AjnI CCWGG 5 cut(s) 782, 1584, 1861, 3516, 4088
AjuI GAANNNNNNNTTGG 6 cut(s) 830, 862, 1352, 1384, 2956, 2988
AluBI AGCT 8 cut(s) 1257, 2562, 2578, 2788, 3145, 3211, 3701, 3891
AluI AGCT 8 cut(s) 1257, 2562, 2578, 2788, 3145, 3211, 3701, 3891
Alw21I GWGCWC 3 cut(s) 1105, 1259, 3386
Alw26I GTCTC 2 cut(s) 2284, 3351
AlwI GGATC 6 cut(s) 1522, 3098, 3128, 3320, 3812, 3832
AlwNI CAGNNNCTG 1 cut(s) 2183
Ama87I CYCGRG 2 cut(s) 2543, 3776
Aor13HI TCCGGA 2 cut(s) 1329, 1736
ApeKI GCWGC 4 cut(s) 3574, 3659, 3676, 3888
ArsI GACNNNNNNTTYG 2 cut(s) 2283, 2315
Asp700I GAANNNNTTC 4 cut(s) 2236, 2814, 3456, 3903
Asp718I GGTACC 1 cut(s) 77
AspLEI GCGC 5 cut(s) 215, 239, 533, 3001, 3228
AspS9I GGNCC 6 cut(s) 617, 1188, 3254, 3720, 3948, 4050
AsuC2I CCSGG 2 cut(s) 1449, 3258
AsuHPI GGTGA 6 cut(s) 49, 590, 1537, 1960, 2057, 2126
AsuII TTCGAA 3 cut(s) 455, 506, 590
AvaI CYCGRG 2 cut(s) 2543, 3776
AvaII GGWCC 4 cut(s) 1188, 3254, 3720, 3948
AxyI CCTNAGG 1 cut(s) 3036
BaeGI GKGCMC 1 cut(s) 1579
BaeI ACNNNNGTAYC 2 cut(s) 3309, 3342
BanI GGYRCC 1 cut(s) 77
BanII GRGCYC 4 cut(s) 1259, 1376, 1563, 2648
BarI GAAGNNNNNNTAC 2 cut(s) 1776, 1808
BbrPI CACGTG 1 cut(s) 3184
BbsI GAAGAC 5 cut(s) 504, 1727, 1747, 3913, 4001
Bbv12I GWGCWC 3 cut(s) 1105, 1259, 3386
BbvCI CCTCAGC 2 cut(s) 3671, 3884
BbvI GCAGC 4 cut(s) 3586, 3663, 3671, 3900
BccI CCATC 9 cut(s) 128, 408, 1738, 1804, 3303, 3611, 3961, 4083, 4105
BceAI ACGGC 4 cut(s) 33, 433, 1614, 2330
BcgI CGANNNNNNTGC 6 cut(s) 161, 195, 522, 556, 1424, 1458
BciT130I CCWGG 5 cut(s) 784, 1586, 1863, 3518, 4090
BclI TGATCA 2 cut(s) 1063, 2931
BcnI CCSGG 2 cut(s) 1449, 3258
BcoDI GTCTC 2 cut(s) 2284, 3351
BfaI CTAG 5 cut(s) 1410, 1815, 3524, 3611, 3960
BfmI CTRYAG 3 cut(s) 891, 1624, 1745
BfoI RGCGCY 2 cut(s) 216, 240
BfrI CTTAAG 1 cut(s) 2639
BfuAI ACCTGC 2 cut(s) 1088, 2076
BglI GCCNNNNNGGC 1 cut(s) 636
BglII AGATCT 3 cut(s) 1649, 1674, 3850
BisI GCNGC 8 cut(s) 46, 240, 243, 294, 3575, 3660, 3677, 3889
BlsI GCNGC 8 cut(s) 47, 241, 244, 295, 3576, 3661, 3678, 3890
BmcAI AGTACT 1 cut(s) 2814
Bme1390I CCNGG 7 cut(s) 784, 1449, 1586, 1863, 3258, 3518, 4090
Bme18I GGWCC 4 cut(s) 1188, 3254, 3720, 3948
BmeT110I CYCGRG 2 cut(s) 2543, 3776
BmgT120I GGNCC 6 cut(s) 617, 1188, 3254, 3720, 3948, 4050
BmiI GGNNCC 4 cut(s) 64, 79, 2702, 3256
BmrFI CCNGG 7 cut(s) 784, 1449, 1586, 1863, 3258, 3518, 4090
BmrI ACTGGG 1 cut(s) 2220
BmuI ACTGGG 1 cut(s) 2220
BoxI GACNNNNGTC 1 cut(s) 2862
BpiI GAAGAC 5 cut(s) 504, 1727, 1747, 3913, 4001
BplI GAGNNNNNCTC 2 cut(s) 464, 496
BpmI CTGGAG 1 cut(s) 557
Bpu10I CCTNAGC 3 cut(s) 1282, 3671, 3884
Bpu14I TTCGAA 3 cut(s) 455, 506, 590
BpuEI CTTGAG 5 cut(s) 1999, 2434, 3161, 3405, 3589
BpuMI CCSGG 2 cut(s) 1449, 3258
BsaAI YACGTR 1 cut(s) 3184
BsaHI GRCGYC 2 cut(s) 938, 2825
BsaJI CCNNGG 5 cut(s) 634, 1260, 1862, 2165, 2241
BsaWI WCCGGW 3 cut(s) 59, 1329, 1736
BsaXI ACNNNNNCTCC 4 cut(s) 1380, 1410, 2986, 3016
Bsc4I CCNNNNNNNGG 9 cut(s) 655, 933, 1302, 1736, 2021, 3517, 3599, 3672, 3729
Bse1I ACTGG 9 cut(s) 274, 540, 1191, 1768, 2026, 2215, 2388, 2939, 3242
Bse21I CCTNAGG 1 cut(s) 3036
Bse3DI GCAATG 1 cut(s) 3490
BseAI TCCGGA 2 cut(s) 1329, 1736
BseBI CCWGG 5 cut(s) 784, 1586, 1863, 3518, 4090
BseDI CCNNGG 5 cut(s) 634, 1260, 1862, 2165, 2241
BseLI CCNNNNNNNGG 9 cut(s) 655, 933, 1302, 1736, 2021, 3517, 3599, 3672, 3729
BseMI GCAATG 1 cut(s) 3490
BseMII CTCAG 8 cut(s) 294, 1347, 2361, 3027, 3137, 3662, 3898, 4070
BseNI ACTGG 9 cut(s) 274, 540, 1191, 1768, 2026, 2215, 2388, 2939, 3242
BseRI GAGGAG 2 cut(s) 58, 237
BseSI GKGCMC 1 cut(s) 1579
BseX3I CGGCCG 3 cut(s) 46, 291, 1445
BseXI GCAGC 4 cut(s) 3586, 3663, 3671, 3900
BseYI CCCAGC 1 cut(s) 209
BsgI GTGCAG 1 cut(s) 2014
Bsh1236I CGCG 1 cut(s) 45
Bsh1285I CGRYCG 4 cut(s) 49, 175, 294, 1448
BshNI GGYRCC 1 cut(s) 77
BsiEI CGRYCG 4 cut(s) 49, 175, 294, 1448
BsiHKAI GWGCWC 3 cut(s) 1105, 1259, 3386
BsiHKCI CYCGRG 2 cut(s) 2543, 3776
BsiSI CCGG 5 cut(s) 60, 1330, 1448, 1737, 3258
BslI CCNNNNNNNGG 9 cut(s) 655, 933, 1302, 1736, 2021, 3517, 3599, 3672, 3729
BsmAI GTCTC 2 cut(s) 2284, 3351
BsmBI CGTCTC 1 cut(s) 2284
BsmI GAATGC 2 cut(s) 1167, 3177
BsoBI CYCGRG 2 cut(s) 2543, 3776
Bsp119I TTCGAA 3 cut(s) 455, 506, 590
Bsp1286I GDGCHC 7 cut(s) 1105, 1259, 1376, 1563, 1579, 2648, 3386
Bsp13I TCCGGA 2 cut(s) 1329, 1736
Bsp19I CCATGG 1 cut(s) 2165
BspCNI CTCAG 8 cut(s) 293, 1348, 2362, 3028, 3138, 3663, 3897, 4071
BspEI TCCGGA 2 cut(s) 1329, 1736
BspFNI CGCG 1 cut(s) 45
BspLI GGNNCC 4 cut(s) 64, 79, 2702, 3256
BspMAI CTGCAG 1 cut(s) 1749
BspMI ACCTGC 2 cut(s) 1088, 2076
BspPI GGATC 6 cut(s) 1522, 3098, 3128, 3320, 3812, 3832
BspQI GCTCTTC 1 cut(s) 487
BspT104I TTCGAA 3 cut(s) 455, 506, 590
BspT107I GGYRCC 1 cut(s) 77
BspTI CTTAAG 1 cut(s) 2639
BsrBI CCGCTC 1 cut(s) 245
BsrDI GCAATG 1 cut(s) 3490
BsrI ACTGG 9 cut(s) 274, 540, 1191, 1768, 2026, 2215, 2388, 2939, 3242
BssECI CCNNGG 5 cut(s) 634, 1260, 1862, 2165, 2241
BssNI GRCGYC 2 cut(s) 938, 2825
BssT1I CCWWGG 2 cut(s) 1260, 2165
Bst2UI CCWGG 5 cut(s) 784, 1586, 1863, 3518, 4090
Bst6I CTCTTC 5 cut(s) 487, 861, 2036, 2857, 2977
BstACI GRCGYC 2 cut(s) 938, 2825
BstAFI CTTAAG 1 cut(s) 2639
BstAPI GCANNNNNTGC 1 cut(s) 4036
BstBAI YACGTR 1 cut(s) 3184
BstBI TTCGAA 3 cut(s) 455, 506, 590
BstC8I GCNNGC 6 cut(s) 1180, 1472, 1859, 2881, 3601, 3931
BstDSI CCRYGG 3 cut(s) 634, 2165, 2241
BstENI CCTNNNNNAGG 1 cut(s) 3670
BstFNI CGCG 1 cut(s) 45
BstH2I RGCGCY 2 cut(s) 216, 240
BstHHI GCGC 5 cut(s) 215, 239, 533, 3001, 3228
BstMAI GTCTC 2 cut(s) 2284, 3351
BstMCI CGRYCG 4 cut(s) 49, 175, 294, 1448
BstMWI GCNNNNNNNGC 6 cut(s) 51, 236, 636, 3656, 3676, 4036
BstNI CCWGG 5 cut(s) 784, 1586, 1863, 3518, 4090
BstNSI RCATGY 6 cut(s) 1861, 2143, 2863, 3031, 3068, 3406
BstPAI GACNNNNGTC 1 cut(s) 2862
BstSCI CCNGG 7 cut(s) 782, 1447, 1584, 1861, 3256, 3516, 4088
BstSFI CTRYAG 3 cut(s) 891, 1624, 1745
BstSLI GKGCMC 1 cut(s) 1579
BstUI CGCG 1 cut(s) 45
BstV1I GCAGC 4 cut(s) 3586, 3663, 3671, 3900
BstV2I GAAGAC 5 cut(s) 504, 1727, 1747, 3913, 4001
BstX2I RGATCY 4 cut(s) 1649, 1674, 3312, 3850
BstXI CCANNNNNNTGG 1 cut(s) 1803
BstYI RGATCY 4 cut(s) 1649, 1674, 3312, 3850
BstZI CGGCCG 3 cut(s) 46, 291, 1445
Bsu36I CCTNAGG 1 cut(s) 3036
BtgI CCRYGG 3 cut(s) 634, 2165, 2241
BtsIMutI CAGTG 8 cut(s) 533, 1198, 1552, 1577, 1603, 1846, 2395, 2506
BveI ACCTGC 2 cut(s) 1088, 2076
Cac8I GCNNGC 6 cut(s) 1180, 1472, 1859, 2881, 3601, 3931
CaiI CAGNNNCTG 1 cut(s) 2183
CfoI GCGC 5 cut(s) 215, 239, 533, 3001, 3228
Cfr13I GGNCC 6 cut(s) 617, 1188, 3254, 3720, 3948, 4050
CseI GACGC 1 cut(s) 946
Csp6I GTAC 7 cut(s) 78, 1784, 1931, 2019, 2476, 2481, 2813
CviQI GTAC 7 cut(s) 78, 1784, 1931, 2019, 2476, 2481, 2813
DraI TTTAAA 1 cut(s) 3789
EaeI YGGCCR 4 cut(s) 46, 291, 446, 1445
EagI CGGCCG 3 cut(s) 46, 291, 1445
Eam1104I CTCTTC 5 cut(s) 487, 861, 2036, 2857, 2977
EarI CTCTTC 5 cut(s) 487, 861, 2036, 2857, 2977
Ecl136II GAGCTC 1 cut(s) 1257
EclXI CGGCCG 3 cut(s) 46, 291, 1445
Eco130I CCWWGG 2 cut(s) 1260, 2165
Eco147I AGGCCT 2 cut(s) 834, 2986
Eco24I GRGCYC 4 cut(s) 1259, 1376, 1563, 2648
Eco32I GATATC 1 cut(s) 3447
Eco47I GGWCC 4 cut(s) 1188, 3254, 3720, 3948
Eco52I CGGCCG 3 cut(s) 46, 291, 1445
Eco53kI GAGCTC 1 cut(s) 1257
Eco57I CTGAAG 3 cut(s) 2804, 2838, 3919
Eco72I CACGTG 1 cut(s) 3184
Eco81I CCTNAGG 1 cut(s) 3036
Eco88I CYCGRG 2 cut(s) 2543, 3776
EcoICRI GAGCTC 1 cut(s) 1257
EcoNI CCTNNNNNAGG 1 cut(s) 3670
EcoO109I RGGNCCY 1 cut(s) 617
EcoRI GAATTC 2 cut(s) 2359, 3686
EcoRII CCWGG 5 cut(s) 782, 1584, 1861, 3516, 4088
EcoRV GATATC 1 cut(s) 3447
EcoT14I CCWWGG 2 cut(s) 1260, 2165
EcoT22I ATGCAT 2 cut(s) 2981, 3060
EcoT38I GRGCYC 4 cut(s) 1259, 1376, 1563, 2648
ErhI CCWWGG 2 cut(s) 1260, 2165
Esp3I CGTCTC 1 cut(s) 2284
FalI AAGNNNNNCTT 4 cut(s) 1937, 1969, 2802, 2834
FauNDI CATATG 1 cut(s) 1547
FbaI TGATCA 2 cut(s) 1063, 2931
Fnu4HI GCNGC 8 cut(s) 46, 240, 243, 294, 3575, 3660, 3677, 3889
FriOI GRGCYC 4 cut(s) 1259, 1376, 1563, 2648
Fsp4HI GCNGC 8 cut(s) 46, 240, 243, 294, 3575, 3660, 3677, 3889
FspBI CTAG 5 cut(s) 1410, 1815, 3524, 3611, 3960
FspI TGCGCA 1 cut(s) 3227
GlaI GCGC 5 cut(s) 214, 238, 532, 3000, 3227
GluI GCNGC 8 cut(s) 46, 240, 243, 294, 3575, 3660, 3677, 3889
GsaI CCCAGC 1 cut(s) 213
GsuI CTGGAG 1 cut(s) 557
HaeII RGCGCY 2 cut(s) 216, 240
HapII CCGG 5 cut(s) 60, 1330, 1448, 1737, 3258
HgaI GACGC 1 cut(s) 946
HhaI GCGC 5 cut(s) 215, 239, 533, 3001, 3228
Hin1I GRCGYC 2 cut(s) 938, 2825
Hin6I GCGC 5 cut(s) 213, 237, 531, 2999, 3226
HinP1I GCGC 5 cut(s) 213, 237, 531, 2999, 3226
HincII GTYRAC 3 cut(s) 1059, 2124, 3586
HindII GTYRAC 3 cut(s) 1059, 2124, 3586
HpaI GTTAAC 2 cut(s) 1059, 2124
HpaII CCGG 5 cut(s) 60, 1330, 1448, 1737, 3258
HphI GGTGA 6 cut(s) 49, 590, 1537, 1960, 2057, 2126
Hpy166II GTNNAC 7 cut(s) 1059, 1415, 1951, 1969, 2124, 2525, 3586
Hpy8I GTNNAC 7 cut(s) 1059, 1415, 1951, 1969, 2124, 2525, 3586
Hpy99I CGWCG 1 cut(s) 2351
HpyCH4IV ACGT 5 cut(s) 312, 385, 2825, 3183, 3272
HpyF10VI GCNNNNNNNGC 6 cut(s) 51, 236, 636, 3656, 3676, 4036
HpySE526I ACGT 5 cut(s) 312, 385, 2825, 3183, 3272
Hsp92I GRCGYC 2 cut(s) 938, 2825
HspAI GCGC 5 cut(s) 213, 237, 531, 2999, 3226
Kpn2I TCCGGA 2 cut(s) 1329, 1736
KpnI GGTACC 1 cut(s) 81
Ksp22I TGATCA 2 cut(s) 1063, 2931
KspAI GTTAAC 2 cut(s) 1059, 2124
LguI GCTCTTC 1 cut(s) 487
LmnI GCTCC 6 cut(s) 97, 250, 260, 1262, 2700, 3298
Lsp1109I GCAGC 4 cut(s) 3586, 3663, 3671, 3900
MaeI CTAG 5 cut(s) 1410, 1815, 3524, 3611, 3960
MaeII ACGT 5 cut(s) 312, 385, 2825, 3183, 3272
MbiI CCGCTC 1 cut(s) 245
MflI RGATCY 4 cut(s) 1649, 1674, 3312, 3850
MhlI GDGCHC 7 cut(s) 1105, 1259, 1376, 1563, 1579, 2648, 3386
MlyI GAGTC 9 cut(s) 24, 548, 698, 878, 1286, 1513, 2381, 2645, 4090
MmeI TCCRAC 5 cut(s) 64, 647, 2239, 2329, 3226
Mph1103I ATGCAT 2 cut(s) 2981, 3060
MroI TCCGGA 2 cut(s) 1329, 1736
MroXI GAANNNNTTC 4 cut(s) 2236, 2814, 3456, 3903
MslI CAYNNNNRTG 5 cut(s) 467, 1568, 2749, 3063, 3182
MspA1I CMGCKG 1 cut(s) 2578
MspCI CTTAAG 1 cut(s) 2639
MspI CCGG 5 cut(s) 60, 1330, 1448, 1737, 3258
MspR9I CCNGG 7 cut(s) 784, 1449, 1586, 1863, 3258, 3518, 4090
Mva1269I GAATGC 2 cut(s) 1167, 3177
MvaI CCWGG 5 cut(s) 784, 1586, 1863, 3518, 4090
MvnI CGCG 1 cut(s) 45
MwoI GCNNNNNNNGC 6 cut(s) 51, 236, 636, 3656, 3676, 4036
NciI CCSGG 2 cut(s) 1449, 3258
NcoI CCATGG 1 cut(s) 2165
NdeI CATATG 1 cut(s) 1547
NlaIV GGNNCC 4 cut(s) 64, 79, 2702, 3256
NmeAIII GCCGAG 2 cut(s) 269, 1423
NmuCI GTSAC 4 cut(s) 308, 1550, 2780, 2944
NsbI TGCGCA 1 cut(s) 3227
NsiI ATGCAT 2 cut(s) 2981, 3060
NspI RCATGY 6 cut(s) 1861, 2143, 2863, 3031, 3068, 3406
NspV TTCGAA 3 cut(s) 455, 506, 590
PaeI GCATGC 1 cut(s) 1861
PaeR7I CTCGAG 2 cut(s) 2543, 3776
PaqCI CACCTGC 2 cut(s) 1088, 2076
PceI AGGCCT 2 cut(s) 834, 2986
PciI ACATGT 1 cut(s) 2859
PciSI GCTCTTC 1 cut(s) 487
PcsI WCGNNNNNNNCGW 1 cut(s) 296
PctI GAATGC 2 cut(s) 1167, 3177
PdmI GAANNNNTTC 4 cut(s) 2236, 2814, 3456, 3903
PflMI CCANNNNNTGG 5 cut(s) 655, 933, 1302, 3517, 3599
PkrI GCNGC 8 cut(s) 47, 241, 244, 295, 3576, 3661, 3678, 3890
PleI GAGTC 9 cut(s) 23, 547, 697, 877, 1285, 1513, 2380, 2645, 4089
PmaCI CACGTG 1 cut(s) 3184
PmlI CACGTG 1 cut(s) 3184
PpsI GAGTC 9 cut(s) 23, 547, 697, 877, 1285, 1513, 2380, 2645, 4089
Ppu21I YACGTR 1 cut(s) 3184
PscI ACATGT 1 cut(s) 2859
PshAI GACNNNNGTC 1 cut(s) 2862
PsiI TTATAA 1 cut(s) 2568
Psp124BI GAGCTC 1 cut(s) 1259
Psp1406I AACGTT 1 cut(s) 3272
Psp6I CCWGG 5 cut(s) 782, 1584, 1861, 3516, 4088
PspCI CACGTG 1 cut(s) 3184
PspFI CCCAGC 1 cut(s) 209
PspGI CCWGG 5 cut(s) 782, 1584, 1861, 3516, 4088
PspN4I GGNNCC 4 cut(s) 64, 79, 2702, 3256
PspPI GGNCC 6 cut(s) 617, 1188, 3254, 3720, 3948, 4050
PspXI VCTCGAGB 1 cut(s) 2543
PstI CTGCAG 1 cut(s) 1749
PstNI CAGNNNCTG 1 cut(s) 2183
PsuI RGATCY 4 cut(s) 1649, 1674, 3312, 3850
PvuII CAGCTG 1 cut(s) 2578
RsaI GTAC 7 cut(s) 79, 1785, 1932, 2020, 2477, 2482, 2814
RsaNI GTAC 7 cut(s) 78, 1784, 1931, 2019, 2476, 2481, 2813
RseI CAYNNNNRTG 5 cut(s) 467, 1568, 2749, 3063, 3182
SacI GAGCTC 1 cut(s) 1259
SapI GCTCTTC 1 cut(s) 487
SatI GCNGC 8 cut(s) 46, 240, 243, 294, 3575, 3660, 3677, 3889
Sau96I GGNCC 6 cut(s) 617, 1188, 3254, 3720, 3948, 4050
ScaI AGTACT 1 cut(s) 2814
SchI GAGTC 9 cut(s) 24, 548, 698, 878, 1286, 1513, 2381, 2645, 4090
ScrFI CCNGG 7 cut(s) 784, 1449, 1586, 1863, 3258, 3518, 4090
SduI GDGCHC 7 cut(s) 1105, 1259, 1376, 1563, 1579, 2648, 3386
SfcI CTRYAG 3 cut(s) 891, 1624, 1745
Sfr274I CTCGAG 2 cut(s) 2543, 3776
SfuI TTCGAA 3 cut(s) 455, 506, 590
SinI GGWCC 4 cut(s) 1188, 3254, 3720, 3948
SlaI CTCGAG 2 cut(s) 2543, 3776
SmiMI CAYNNNNRTG 5 cut(s) 467, 1568, 2749, 3063, 3182
SmlI CTYRAG 8 cut(s) 1978, 2413, 2543, 2639, 3140, 3420, 3568, 3776
SmoI CTYRAG 8 cut(s) 1978, 2413, 2543, 2639, 3140, 3420, 3568, 3776
SphI GCATGC 1 cut(s) 1861
SseBI AGGCCT 2 cut(s) 834, 2986
SspI AATATT 1 cut(s) 817
SspMI CTAG 5 cut(s) 1410, 1815, 3524, 3611, 3960
SstI GAGCTC 1 cut(s) 1259
StuI AGGCCT 2 cut(s) 834, 2986
StyD4I CCNGG 7 cut(s) 782, 1447, 1584, 1861, 3256, 3516, 4088
StyI CCWWGG 2 cut(s) 1260, 2165
TaiI ACGT 5 cut(s) 315, 388, 2828, 3186, 3275
TatI WGTACW 2 cut(s) 2018, 2812
TauI GCSGC 4 cut(s) 48, 242, 245, 296
TscAI CASTG 8 cut(s) 540, 1198, 1559, 1584, 1603, 1846, 2395, 2506
TseFI GTSAC 4 cut(s) 308, 1550, 2780, 2944
TseI GCWGC 4 cut(s) 3574, 3659, 3676, 3888
Tsp45I GTSAC 4 cut(s) 308, 1550, 2780, 2944
TspGWI ACGGA 7 cut(s) 100, 366, 623, 1977, 2230, 2308, 4031
TspRI CASTG 8 cut(s) 540, 1198, 1559, 1584, 1603, 1846, 2395, 2506
Van91I CCANNNNNTGG 5 cut(s) 655, 933, 1302, 3517, 3599
Vha464I CTTAAG 1 cut(s) 2639
VpaK11BI GGWCC 4 cut(s) 1188, 3254, 3720, 3948
XagI CCTNNNNNAGG 1 cut(s) 3670
XbaI TCTAGA 2 cut(s) 3523, 3610
XceI RCATGY 6 cut(s) 1861, 2143, 2863, 3031, 3068, 3406
XcmI CCANNNNNNNNNTGG 2 cut(s) 1198, 3636
XhoI CTCGAG 2 cut(s) 2543, 3776
XmnI GAANNNNTTC 4 cut(s) 2236, 2814, 3456, 3903
XspI CTAG 5 cut(s) 1410, 1815, 3524, 3611, 3960
ZraI GACGTC 1 cut(s) 2826
ZrmI AGTACT 1 cut(s) 2814
Zsp2I ATGCAT 2 cut(s) 2981, 3060
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.