MD08G1077300.v1.1

Protein of unknown function (DUF3054)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0011949
Physical Location & Seq
Reverse (-)
6379358 .. 6381749
2392 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1077300.v1.1.491

Sequence Viewer

Length: 957 bp
ATGAAATATCCTCAAAGCGAAAGGCCCAAAAAGAAGAAGAAGAAGAAGAAGAAAAGGGTCTGGGCAGCGCTGCGCTTGTGTGGGTGTTGCTGTAGCGAACGAGTGTGGGCAACTGAGCAACCCACGGAACTGAAGGCTCAAATGCTAGTGCTCGGCCAAGCTCCCGGCGGAGCCTTATCAGCCCGGCGGTGTTCTCTCACCTCCCCGCCAAAATTCTCAAACCCAAACCCTCCCCTCTTCTCTTCCCGCAGACCCAGCAGATTCTTCTCCAATGTAAAACCTTCAAAATCCCTCAATATCACGCTCGTAAAGGCCAACGGAGGCGTCGACTCGGCTTCCTCCACTGCCAAACAGGGCTTCTCCGCCTCATCACCTCCTCCTCCTCCTCCTCCGCCTTCCGTCGATAACCAACCAGTTTTCGTGGGTCAGGAGAACATACATCTAGAAGGCGTGATTCAGTTCGAGAAGTCCGATTCTTCTGCTCGCTTGCGCAAATGGGGTCGTGTAGCATTGCTTGCTGGTGGGGATGTTTTGGCATTGCTTACGTTTGCTGCAATTGGAAGATTCAGCCATGGATTCTCCGTTTTCGATTTCGAAACACTGCGCACGGCTGACCCTTTTATTGCTGGGTGGTTTTTGAGTGCTTATTTCCTAGGAGGATTTGGTGAGGATGGTCGTGGAGTCAATGGACAATCGAAGGCTCTTACCGCTACGGCTAAGTCATGGGCCCTGGGAATTCCACTTGGGATAATCATAAGGGCATCAACAGCTGGCCACATTCCGCCAGTCAAATTCATACTTGTAACAATGGGGAGCACTGCTGTTTTACTTATTGGATGGAGATCACTATTGTATAAGTTTCTTCCCGGTGATAAGAGCAAGAAGAGCGATGTATATCGCCGCGGTAGCCCATTTGAATTGTTTGAGTTGCTCACTTCATTAATACGAAGGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3054

Protein Analysis

319

Amino Acids

34.65

Weight (kDa)

10.33

Isoelectric Point (pI)

53.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3054 PF11255 174 - 283 1.1e-29 Protein of unknown function (DUF3054)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011949)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44920
fragaria_vesca FvH4_2g32650
malus_domestica MD08G1077300.v1.1 MD15G1064800.v1.1
prunus_persica Prupe.1G418700_v2.0.a1
pyrus_communis pycom08g06420 pycom15g06150
rosa_chinensis RchiOBHm_Chr6g0310081
rosa_laevigata RLG00000010513
rosa_multiflora Rmu_sc0000294.1_g000021 Rmu_sc0038452.1_g000001
rosa_roxburghii Rroxscaffold_7G00158900
rosa_rugosa Rorug06G0377200 Rorug06G0377200
rosa_samantha Rh6AG491100 Rh6BG500500 Rh6CG505400 Rh6DG491400
rosa_wichuraiana Rw6G042730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 491, 605
AccI GTMKAC 1 cut(s) 327
AccII CGCG 1 cut(s) 903
AcoI YGGCCR 2 cut(s) 154, 772
AcsI RAATTY 3 cut(s) 212, 735, 791
AcuI CTGAAG 1 cut(s) 152
AcyI GRCGYC 1 cut(s) 324
AfeI AGCGCT 1 cut(s) 69
AgsI TTSAA 2 cut(s) 285, 917
AjnI CCWGG 1 cut(s) 729
AjuI GAANNNNNNNTTGG 2 cut(s) 341, 373
AluBI AGCT 2 cut(s) 161, 770
AluI AGCT 2 cut(s) 161, 770
Alw21I GWGCWC 2 cut(s) 153, 818
Aor51HI AGCGCT 1 cut(s) 69
AoxI GGCC 5 cut(s) 23, 154, 312, 726, 772
ApaI GGGCCC 1 cut(s) 730
ApeKI GCWGC 3 cut(s) 65, 70, 551
ApoI RAATTY 3 cut(s) 212, 735, 791
AseI ATTAAT 1 cut(s) 941
AspA2I CCTAGG 1 cut(s) 652
AspLEI GCGC 4 cut(s) 70, 75, 492, 606
AspS9I GGNCC 3 cut(s) 24, 726, 727
AsuC2I CCSGG 3 cut(s) 165, 184, 867
AsuHPI GGTGA 4 cut(s) 190, 363, 677, 881
AsuII TTCGAA 1 cut(s) 594
AvrII CCTAGG 1 cut(s) 652
BaeGI GKGCMC 1 cut(s) 730
BalI TGGCCA 1 cut(s) 774
BanII GRGCYC 1 cut(s) 730
Bbv12I GWGCWC 2 cut(s) 153, 818
BbvI GCAGC 3 cut(s) 57, 77, 538
BccI CCATC 2 cut(s) 665, 831
BceAI ACGGC 2 cut(s) 624, 729
BcgI CGANNNNNNTGC 2 cut(s) 461, 495
BciT130I CCWGG 1 cut(s) 731
BcnI CCSGG 3 cut(s) 165, 184, 867
BfaI CTAG 3 cut(s) 146, 443, 653
BfmI CTRYAG 1 cut(s) 91
BfoI RGCGCY 1 cut(s) 71
BisI GCNGC 4 cut(s) 66, 71, 552, 901
BlnI CCTAGG 1 cut(s) 652
BlsI GCNGC 4 cut(s) 67, 72, 553, 902
Bme1390I CCNGG 4 cut(s) 165, 184, 731, 867
BmgT120I GGNCC 3 cut(s) 24, 726, 727
BmiI GGNNCC 2 cut(s) 172, 728
BmrFI CCNGG 4 cut(s) 165, 184, 731, 867
BmsI GCATC 1 cut(s) 770
Bpu14I TTCGAA 1 cut(s) 594
BpuMI CCSGG 3 cut(s) 165, 184, 867
BsaBI GATNNNNATC 2 cut(s) 841, 894
BsaHI GRCGYC 1 cut(s) 324
BsaJI CCNNGG 6 cut(s) 123, 571, 652, 729, 730, 901
Bse1I ACTGG 2 cut(s) 413, 785
Bse3DI GCAATG 2 cut(s) 509, 536
Bse8I GATNNNNATC 2 cut(s) 841, 894
BseBI CCWGG 1 cut(s) 731
BseDI CCNNGG 6 cut(s) 123, 571, 652, 729, 730, 901
BseGI GGATG 3 cut(s) 532, 676, 842
BseJI GATNNNNATC 2 cut(s) 841, 894
BseMI GCAATG 2 cut(s) 509, 536
BseMII CTCAG 1 cut(s) 105
BseNI ACTGG 2 cut(s) 413, 785
BseRI GAGGAG 5 cut(s) 366, 369, 372, 375, 378
BseSI GKGCMC 1 cut(s) 730
BseXI GCAGC 3 cut(s) 57, 77, 538
BseYI CCCAGC 2 cut(s) 254, 626
Bsh1236I CGCG 1 cut(s) 903
BshFI GGCC 5 cut(s) 25, 156, 314, 728, 774
BsiHKAI GWGCWC 2 cut(s) 153, 818
BsiSI CCGG 3 cut(s) 165, 184, 867
BsnI GGCC 5 cut(s) 25, 156, 314, 728, 774
Bsp119I TTCGAA 1 cut(s) 594
Bsp120I GGGCCC 1 cut(s) 726
Bsp1286I GDGCHC 3 cut(s) 153, 730, 818
Bsp143I GATC 1 cut(s) 842
Bsp19I CCATGG 1 cut(s) 571
BspANI GGCC 5 cut(s) 25, 156, 314, 728, 774
BspCNI CTCAG 1 cut(s) 106
BspFNI CGCG 1 cut(s) 903
BspLI GGNNCC 2 cut(s) 172, 728
BspQI GCTCTTC 1 cut(s) 878
BspT104I TTCGAA 1 cut(s) 594
BsrDI GCAATG 2 cut(s) 509, 536
BsrI ACTGG 2 cut(s) 413, 785
BssECI CCNNGG 6 cut(s) 123, 571, 652, 729, 730, 901
BssMI GATC 1 cut(s) 842
BssNI GRCGYC 1 cut(s) 324
BssT1I CCWWGG 2 cut(s) 571, 652
Bst2UI CCWGG 1 cut(s) 731
Bst6I CTCTTC 3 cut(s) 242, 247, 878
BstACI GRCGYC 1 cut(s) 324
BstAPI GCANNNNNTGC 1 cut(s) 515
BstBI TTCGAA 1 cut(s) 594
BstC8I GCNNGC 4 cut(s) 484, 488, 516, 772
BstDEI CTNAG 2 cut(s) 114, 717
BstDSI CCRYGG 3 cut(s) 123, 571, 901
BstF5I GGATG 3 cut(s) 532, 676, 842
BstFNI CGCG 1 cut(s) 903
BstH2I RGCGCY 1 cut(s) 71
BstHHI GCGC 4 cut(s) 70, 75, 492, 606
BstKTI GATC 1 cut(s) 845
BstMBI GATC 1 cut(s) 842
BstMWI GCNNNNNNNGC 7 cut(s) 179, 255, 515, 707, 767, 885, 906
BstNI CCWGG 1 cut(s) 731
BstSCI CCNGG 4 cut(s) 163, 182, 729, 865
BstSFI CTRYAG 1 cut(s) 91
BstSLI GKGCMC 1 cut(s) 730
BstUI CGCG 1 cut(s) 903
BstV1I GCAGC 3 cut(s) 57, 77, 538
BsuRI GGCC 5 cut(s) 25, 156, 314, 728, 774
BtgI CCRYGG 3 cut(s) 123, 571, 901
BtgZI GCGATG 1 cut(s) 903
BtsCI GGATG 3 cut(s) 532, 676, 842
BtsI GCAGTG 3 cut(s) 342, 599, 816
BtsIMutI CAGTG 3 cut(s) 342, 599, 816
Cac8I GCNNGC 4 cut(s) 484, 488, 516, 772
CfoI GCGC 4 cut(s) 70, 75, 492, 606
Cfr13I GGNCC 3 cut(s) 24, 726, 727
Cfr42I CCGCGG 1 cut(s) 904
CseI GACGC 1 cut(s) 313
CviAII CATG 2 cut(s) 572, 723
DdeI CTNAG 2 cut(s) 114, 717
DpnI GATC 1 cut(s) 844
DpnII GATC 1 cut(s) 842
EaeI YGGCCR 2 cut(s) 154, 772
Eam1104I CTCTTC 3 cut(s) 242, 247, 878
EarI CTCTTC 3 cut(s) 242, 247, 878
EciI GGCGGA 4 cut(s) 183, 352, 381, 771
Eco130I CCWWGG 2 cut(s) 571, 652
Eco24I GRGCYC 1 cut(s) 730
Eco47III AGCGCT 1 cut(s) 69
Eco57I CTGAAG 1 cut(s) 152
EcoO109I RGGNCCY 1 cut(s) 727
EcoRI GAATTC 1 cut(s) 735
EcoRII CCWGG 1 cut(s) 729
EcoT14I CCWWGG 2 cut(s) 571, 652
EcoT38I GRGCYC 1 cut(s) 730
ErhI CCWWGG 2 cut(s) 571, 652
FaeI CATG 2 cut(s) 575, 726
FaiI YATR 7 cut(s) 437, 573, 724, 755, 797, 855, 895
FatI CATG 2 cut(s) 571, 722
FauI CCCGC 2 cut(s) 213, 254
FblI GTMKAC 1 cut(s) 327
Fnu4HI GCNGC 4 cut(s) 66, 71, 552, 901
FokI GGATG 3 cut(s) 539, 683, 849
FriOI GRGCYC 1 cut(s) 730
Fsp4HI GCNGC 4 cut(s) 66, 71, 552, 901
FspBI CTAG 3 cut(s) 146, 443, 653
FspI TGCGCA 2 cut(s) 491, 605
GlaI GCGC 4 cut(s) 69, 74, 491, 605
GluI GCNGC 4 cut(s) 66, 71, 552, 901
GsaI CCCAGC 2 cut(s) 258, 630
HaeII RGCGCY 1 cut(s) 71
HaeIII GGCC 5 cut(s) 25, 156, 314, 728, 774
HapII CCGG 3 cut(s) 165, 184, 867
HgaI GACGC 1 cut(s) 313
HhaI GCGC 4 cut(s) 70, 75, 492, 606
Hin1I GRCGYC 1 cut(s) 324
Hin1II CATG 2 cut(s) 575, 726
Hin6I GCGC 4 cut(s) 68, 73, 490, 604
HinP1I GCGC 4 cut(s) 68, 73, 490, 604
HincII GTYRAC 1 cut(s) 328
HindII GTYRAC 1 cut(s) 328
HinfI GANTC 7 cut(s) 261, 329, 454, 473, 564, 576, 681
HpaII CCGG 3 cut(s) 165, 184, 867
HphI GGTGA 4 cut(s) 190, 363, 677, 881
Hpy166II GTNNAC 1 cut(s) 328
Hpy188I TCNGA 1 cut(s) 472
Hpy188III TCNNGA 3 cut(s) 428, 443, 463
Hpy8I GTNNAC 1 cut(s) 328
Hpy99I CGWCG 2 cut(s) 329, 404
HpyAV CCTTC 6 cut(s) 127, 291, 405, 440, 691, 942
HpyCH4IV ACGT 1 cut(s) 545
HpyCH4V TGCA 1 cut(s) 554
HpyF10VI GCNNNNNNNGC 7 cut(s) 179, 255, 515, 707, 767, 885, 906
HpyF3I CTNAG 2 cut(s) 114, 717
HpySE526I ACGT 1 cut(s) 545
Hsp92I GRCGYC 1 cut(s) 324
Hsp92II CATG 2 cut(s) 575, 726
HspAI GCGC 4 cut(s) 68, 73, 490, 604
KspI CCGCGG 1 cut(s) 904
Kzo9I GATC 1 cut(s) 842
LguI GCTCTTC 1 cut(s) 878
LmnI GCTCC 3 cut(s) 166, 170, 813
Lsp1109I GCAGC 3 cut(s) 57, 77, 538
LweI GCATC 1 cut(s) 770
MaeI CTAG 3 cut(s) 146, 443, 653
MaeII ACGT 1 cut(s) 545
MaeIII GTNAC 1 cut(s) 802
MalI GATC 1 cut(s) 844
MboI GATC 1 cut(s) 842
MfeI CAATTG 1 cut(s) 555
MhlI GDGCHC 3 cut(s) 153, 730, 818
MlsI TGGCCA 1 cut(s) 774
MluCI AATT 5 cut(s) 212, 555, 735, 791, 917
MluNI TGGCCA 1 cut(s) 774
MlyI GAGTC 2 cut(s) 323, 690
Mox20I TGGCCA 1 cut(s) 774
MscI TGGCCA 1 cut(s) 774
MseI TTAA 1 cut(s) 941
Msp20I TGGCCA 1 cut(s) 774
MspA1I CMGCKG 2 cut(s) 770, 903
MspI CCGG 3 cut(s) 165, 184, 867
MspR9I CCNGG 4 cut(s) 165, 184, 731, 867
MteI GCGCNGCGC 1 cut(s) 71
MunI CAATTG 1 cut(s) 555
MvaI CCWGG 1 cut(s) 731
MvnI CGCG 1 cut(s) 903
MwoI GCNNNNNNNGC 7 cut(s) 179, 255, 515, 707, 767, 885, 906
NciI CCSGG 3 cut(s) 165, 184, 867
NcoI CCATGG 1 cut(s) 571
NdeII GATC 1 cut(s) 842
NlaIII CATG 2 cut(s) 575, 726
NlaIV GGNNCC 2 cut(s) 172, 728
NmeAIII GCCGAG 2 cut(s) 132, 311
NsbI TGCGCA 2 cut(s) 491, 605
NspV TTCGAA 1 cut(s) 594
PasI CCCWGGG 1 cut(s) 730
PciSI GCTCTTC 1 cut(s) 878
PcsI WCGNNNNNNNCGW 2 cut(s) 324, 468
PfeI GAWTC 5 cut(s) 261, 454, 473, 564, 576
PkrI GCNGC 4 cut(s) 67, 72, 553, 902
PleI GAGTC 2 cut(s) 323, 689
PpsI GAGTC 2 cut(s) 323, 689
PshBI ATTAAT 1 cut(s) 941
Psp6I CCWGG 1 cut(s) 729
PspFI CCCAGC 2 cut(s) 254, 626
PspGI CCWGG 1 cut(s) 729
PspN4I GGNNCC 2 cut(s) 172, 728
PspOMI GGGCCC 1 cut(s) 726
PspPI GGNCC 3 cut(s) 24, 726, 727
PvuII CAGCTG 1 cut(s) 770
SacII CCGCGG 1 cut(s) 904
SalI GTCGAC 1 cut(s) 326
SapI GCTCTTC 1 cut(s) 878
SaqAI TTAA 1 cut(s) 941
SatI GCNGC 4 cut(s) 66, 71, 552, 901
Sau3AI GATC 1 cut(s) 842
Sau96I GGNCC 3 cut(s) 24, 726, 727
SchI GAGTC 2 cut(s) 323, 690
ScrFI CCNGG 4 cut(s) 165, 184, 731, 867
SduI GDGCHC 3 cut(s) 153, 730, 818
SetI ASST 7 cut(s) 163, 203, 283, 376, 548, 772, 953
SfaNI GCATC 1 cut(s) 770
SfcI CTRYAG 1 cut(s) 91
Sfr303I CCGCGG 1 cut(s) 904
SfuI TTCGAA 1 cut(s) 594
SgrBI CCGCGG 1 cut(s) 904
Sse9I AATT 5 cut(s) 212, 555, 735, 791, 917
SspMI CTAG 3 cut(s) 146, 443, 653
StyD4I CCNGG 4 cut(s) 163, 182, 729, 865
StyI CCWWGG 2 cut(s) 571, 652
TaiI ACGT 1 cut(s) 548
TaqI TCGA 6 cut(s) 327, 402, 462, 588, 594, 695
TasI AATT 5 cut(s) 212, 555, 735, 791, 917
TauI GCSGC 1 cut(s) 903
TfiI GAWTC 5 cut(s) 261, 454, 473, 564, 576
Tru1I TTAA 1 cut(s) 941
Tru9I TTAA 1 cut(s) 941
TscAI CASTG 3 cut(s) 349, 606, 823
TseI GCWGC 3 cut(s) 65, 70, 551
TspDTI ATGAA 3 cut(s) 17, 784, 927
TspGWI ACGGA 4 cut(s) 140, 333, 388, 571
TspRI CASTG 3 cut(s) 349, 606, 823
VspI ATTAAT 1 cut(s) 941
XapI RAATTY 3 cut(s) 212, 735, 791
XbaI TCTAGA 1 cut(s) 442
XmaJI CCTAGG 1 cut(s) 652
XmiI GTMKAC 1 cut(s) 327
XspI CTAG 3 cut(s) 146, 443, 653
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.