MD15G1064800.v1.1

Protein of unknown function (DUF3054)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0011949
Physical Location & Seq
Reverse (-)
4506879 .. 4509180
2302 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1064800.v1.1.491

Sequence Viewer

Length: 825 bp
ATGCTAGTGCTCGGCCAAGCTCCCGGCGGAGCCCTATCAACTCAGCGGTGTTATCTTACCTCCCCGCCAAAATTCTCAAACCCAAACCCTCCGCTCTTCTCTACCCACAGACCCAGCAGATTCTTCTCCAATGTAAACCCTTCAAAATCTCTCAATATCACGCTCGCAAAGGCCGACGGAGGCGTCGATTCGGCTTCCTCCGCTGACAAACGGGGCTTCTCGGCCTCATCAACTCCTCCTCCTCCTCCTCCTCCTCCTCCGCCTTCCGTCGATAACCGACCAGTTTTCGTGGGTCAGGAGAATGTTCCTCTAGAAGGCGTGATTCAGTTCGAGAAGCCCGATTCTTCTGCTCGTGTACGCAAATGGGGTCATGTGGCATTGCTTGCTGGTGGGGATGTTTTGGCATTGCTTACGTTTGCTGCAATTGGAAGATTCAGCCATGGATTCCCCGTTTTCGAATTCGAAACACTTCGCACGGCTGACCCTTTCATTGCTGGATGGTTTTTGAGTGCATATTTTCTCGGAGGCTTTGGTGAGGATGGACGTGGAGTCAATGGACAATCCAAGGCACTTACCGCTACGGCTAAGTCATGGGCCTTGGGAATTCCACTTGGGATAATCATAAGGGCATCGACGGCTGGCCACATTCCACCAATCAAATTCGTACTTGCAACAATGGGGAGCACTGGTGTTTTACTTATTGGATGGAGAGCACTTCTGTATAAGTTTCTTCCCGGCGATAAGAGCAAGAAGAGTGATGTGTATCGCCGCGGTAGCCCATTTGAATTGTTTGAGTTGCTCACGTCGTTAGTACGAAGGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3054

Protein Analysis

275

Amino Acids

29.36

Weight (kDa)

10.04

Isoelectric Point (pI)

42.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3054 PF11255 130 - 239 2.1e-28 Protein of unknown function (DUF3054)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011949)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44920
fragaria_vesca FvH4_2g32650
malus_domestica MD08G1077300.v1.1 MD15G1064800.v1.1
prunus_persica Prupe.1G418700_v2.0.a1
pyrus_communis pycom08g06420 pycom15g06150
rosa_chinensis RchiOBHm_Chr6g0310081
rosa_laevigata RLG00000010513
rosa_multiflora Rmu_sc0000294.1_g000021 Rmu_sc0038452.1_g000001
rosa_roxburghii Rroxscaffold_7G00158900
rosa_rugosa Rorug06G0377200 Rorug06G0377200
rosa_samantha Rh6AG491100 Rh6BG500500 Rh6CG505400 Rh6DG491400
rosa_wichuraiana Rw6G042730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 182
AccBSI CCGCTC 1 cut(s) 94
AccII CGCG 1 cut(s) 771
AciI CCGC 9 cut(s) 27, 46, 65, 92, 201, 260, 576, 769, 771
AcoI YGGCCR 2 cut(s) 13, 640
AcsI RAATTY 4 cut(s) 71, 458, 603, 659
AcyI GRCGYC 1 cut(s) 183
AfaI GTAC 3 cut(s) 357, 666, 813
AfiI CCNNNNNNNGG 1 cut(s) 314
AgsI TTSAA 2 cut(s) 144, 785
AhdI GACNNNNNGTC 1 cut(s) 548
AjiI CACGTC 2 cut(s) 545, 804
AluBI AGCT 1 cut(s) 20
AluI AGCT 1 cut(s) 20
Alw21I GWGCWC 3 cut(s) 12, 686, 715
AoxI GGCC 5 cut(s) 13, 171, 222, 594, 640
ApeKI GCWGC 1 cut(s) 419
ApoI RAATTY 4 cut(s) 71, 458, 603, 659
Asp700I GAANNNNTTC 1 cut(s) 468
AspS9I GGNCC 1 cut(s) 594
AsuC2I CCSGG 2 cut(s) 24, 735
AsuHPI GGTGA 1 cut(s) 545
AsuII TTCGAA 2 cut(s) 456, 462
BalI TGGCCA 1 cut(s) 642
BanII GRGCYC 1 cut(s) 34
BauI CACGAG 1 cut(s) 351
Bbv12I GWGCWC 3 cut(s) 12, 686, 715
BbvI GCAGC 1 cut(s) 406
BccI CCATC 3 cut(s) 492, 533, 699
BceAI ACGGC 3 cut(s) 492, 597, 651
BcgI CGANNNNNNTGC 2 cut(s) 329, 363
BcnI CCSGG 2 cut(s) 24, 735
BfaI CTAG 2 cut(s) 5, 311
BisI GCNGC 2 cut(s) 420, 769
BlsI GCNGC 2 cut(s) 421, 770
Bme1390I CCNGG 2 cut(s) 24, 735
BmeRI GACNNNNNGTC 1 cut(s) 548
BmgBI CACGTC 2 cut(s) 545, 804
BmgT120I GGNCC 1 cut(s) 594
BmiI GGNNCC 1 cut(s) 31
BmrFI CCNGG 2 cut(s) 24, 735
BmsI GCATC 1 cut(s) 638
Bpu14I TTCGAA 2 cut(s) 456, 462
BpuMI CCSGG 2 cut(s) 24, 735
BsaBI GATNNNNATC 1 cut(s) 762
BsaHI GRCGYC 1 cut(s) 183
BsaJI CCNNGG 4 cut(s) 439, 564, 597, 769
BsaXI ACNNNNNCTCC 4 cut(s) 223, 253, 673, 703
Bsc4I CCNNNNNNNGG 1 cut(s) 314
Bse1I ACTGG 2 cut(s) 281, 691
Bse3DI GCAATG 3 cut(s) 377, 404, 489
Bse8I GATNNNNATC 1 cut(s) 762
BseDI CCNNGG 4 cut(s) 439, 564, 597, 769
BseGI GGATG 4 cut(s) 400, 503, 544, 710
BseJI GATNNNNATC 1 cut(s) 762
BseLI CCNNNNNNNGG 1 cut(s) 314
BseMI GCAATG 3 cut(s) 377, 404, 489
BseMII CTCAG 1 cut(s) 56
BseNI ACTGG 2 cut(s) 281, 691
BseRI GAGGAG 8 cut(s) 225, 228, 231, 234, 237, 240, 243, 246
BseXI GCAGC 1 cut(s) 406
BseYI CCCAGC 1 cut(s) 113
Bsh1236I CGCG 1 cut(s) 771
BshFI GGCC 5 cut(s) 15, 173, 224, 596, 642
BsiHKAI GWGCWC 3 cut(s) 12, 686, 715
BsiSI CCGG 2 cut(s) 24, 735
BslI CCNNNNNNNGG 1 cut(s) 314
BsnI GGCC 5 cut(s) 15, 173, 224, 596, 642
Bsp119I TTCGAA 2 cut(s) 456, 462
Bsp1286I GDGCHC 4 cut(s) 12, 34, 686, 715
Bsp19I CCATGG 1 cut(s) 439
BspACI CCGC 9 cut(s) 27, 46, 65, 92, 201, 260, 576, 769, 771
BspANI GGCC 5 cut(s) 15, 173, 224, 596, 642
BspCNI CTCAG 1 cut(s) 55
BspFNI CGCG 1 cut(s) 771
BspLI GGNNCC 1 cut(s) 31
BspQI GCTCTTC 1 cut(s) 101
BspT104I TTCGAA 2 cut(s) 456, 462
BsrBI CCGCTC 1 cut(s) 94
BsrDI GCAATG 3 cut(s) 377, 404, 489
BsrI ACTGG 2 cut(s) 281, 691
BssECI CCNNGG 4 cut(s) 439, 564, 597, 769
BssNI GRCGYC 1 cut(s) 183
BssSI CACGAG 1 cut(s) 351
BssT1I CCWWGG 3 cut(s) 439, 564, 597
Bst2BI CACGAG 1 cut(s) 351
Bst6I CTCTTC 2 cut(s) 101, 746
BstACI GRCGYC 1 cut(s) 183
BstAPI GCANNNNNTGC 1 cut(s) 383
BstBI TTCGAA 2 cut(s) 456, 462
BstC8I GCNNGC 3 cut(s) 165, 384, 640
BstDEI CTNAG 2 cut(s) 42, 585
BstDSI CCRYGG 2 cut(s) 439, 769
BstENI CCTNNNNNAGG 1 cut(s) 312
BstF5I GGATG 4 cut(s) 400, 503, 544, 710
BstFNI CGCG 1 cut(s) 771
BstMWI GCNNNNNNNGC 6 cut(s) 200, 383, 575, 635, 744, 774
BstSCI CCNGG 2 cut(s) 22, 733
BstUI CGCG 1 cut(s) 771
BstV1I GCAGC 1 cut(s) 406
BsuRI GGCC 5 cut(s) 15, 173, 224, 596, 642
BtgI CCRYGG 2 cut(s) 439, 769
BtrI CACGTC 2 cut(s) 545, 804
BtsCI GGATG 4 cut(s) 400, 503, 544, 710
BtsIMutI CAGTG 1 cut(s) 684
Cac8I GCNNGC 3 cut(s) 165, 384, 640
Cfr13I GGNCC 1 cut(s) 594
Cfr42I CCGCGG 1 cut(s) 772
CseI GACGC 1 cut(s) 172
Csp6I GTAC 3 cut(s) 356, 665, 812
CviAII CATG 3 cut(s) 371, 440, 591
CviQI GTAC 3 cut(s) 356, 665, 812
DdeI CTNAG 2 cut(s) 42, 585
DrdI GACNNNNNNGTC 1 cut(s) 182
DriI GACNNNNNGTC 1 cut(s) 548
DseDI GACNNNNNNGTC 1 cut(s) 182
EaeI YGGCCR 2 cut(s) 13, 640
Eam1104I CTCTTC 2 cut(s) 101, 746
Eam1105I GACNNNNNGTC 1 cut(s) 548
EarI CTCTTC 2 cut(s) 101, 746
EciI GGCGGA 2 cut(s) 42, 249
Eco130I CCWWGG 3 cut(s) 439, 564, 597
Eco24I GRGCYC 1 cut(s) 34
EcoNI CCTNNNNNAGG 1 cut(s) 312
EcoRI GAATTC 2 cut(s) 458, 603
EcoT14I CCWWGG 3 cut(s) 439, 564, 597
EcoT38I GRGCYC 1 cut(s) 34
ErhI CCWWGG 3 cut(s) 439, 564, 597
FaeI CATG 3 cut(s) 374, 443, 594
FaiI YATR 6 cut(s) 372, 441, 514, 592, 623, 723
FatI CATG 3 cut(s) 370, 439, 590
FauI CCCGC 1 cut(s) 72
Fnu4HI GCNGC 2 cut(s) 420, 769
FokI GGATG 4 cut(s) 407, 510, 551, 717
FriOI GRGCYC 1 cut(s) 34
Fsp4HI GCNGC 2 cut(s) 420, 769
FspBI CTAG 2 cut(s) 5, 311
GluI GCNGC 2 cut(s) 420, 769
GsaI CCCAGC 1 cut(s) 117
HaeIII GGCC 5 cut(s) 15, 173, 224, 596, 642
HapII CCGG 2 cut(s) 24, 735
HgaI GACGC 1 cut(s) 172
Hin1I GRCGYC 1 cut(s) 183
Hin1II CATG 3 cut(s) 374, 443, 594
HinfI GANTC 7 cut(s) 120, 188, 322, 341, 432, 444, 549
HpaII CCGG 2 cut(s) 24, 735
HphI GGTGA 1 cut(s) 545
Hpy166II GTNNAC 2 cut(s) 136, 356
Hpy188I TCNGA 1 cut(s) 524
Hpy188III TCNNGA 3 cut(s) 296, 311, 331
Hpy8I GTNNAC 2 cut(s) 136, 356
Hpy99I CGWCG 5 cut(s) 179, 188, 272, 637, 808
HpyAV CCTTC 4 cut(s) 150, 273, 308, 810
HpyCH4IV ACGT 3 cut(s) 413, 544, 803
HpyCH4V TGCA 3 cut(s) 422, 512, 671
HpyF10VI GCNNNNNNNGC 6 cut(s) 200, 383, 575, 635, 744, 774
HpyF3I CTNAG 2 cut(s) 42, 585
HpySE526I ACGT 3 cut(s) 413, 544, 803
Hsp92I GRCGYC 1 cut(s) 183
Hsp92II CATG 3 cut(s) 374, 443, 594
KspI CCGCGG 1 cut(s) 772
LguI GCTCTTC 1 cut(s) 101
LmnI GCTCC 3 cut(s) 25, 29, 681
LpnPI CCDG 9 cut(s) 37, 127, 281, 294, 372, 480, 624, 672, 748
Lsp1109I GCAGC 1 cut(s) 406
LweI GCATC 1 cut(s) 638
MaeI CTAG 2 cut(s) 5, 311
MaeII ACGT 3 cut(s) 413, 544, 803
MbiI CCGCTC 1 cut(s) 94
MboII GAAGA 6 cut(s) 88, 115, 336, 441, 722, 763
MfeI CAATTG 1 cut(s) 423
MhlI GDGCHC 4 cut(s) 12, 34, 686, 715
MlsI TGGCCA 1 cut(s) 642
MluCI AATT 6 cut(s) 71, 423, 458, 603, 659, 785
MluNI TGGCCA 1 cut(s) 642
MlyI GAGTC 1 cut(s) 558
Mox20I TGGCCA 1 cut(s) 642
MroXI GAANNNNTTC 1 cut(s) 468
MscI TGGCCA 1 cut(s) 642
Msp20I TGGCCA 1 cut(s) 642
MspA1I CMGCKG 3 cut(s) 46, 203, 771
MspI CCGG 2 cut(s) 24, 735
MspR9I CCNGG 2 cut(s) 24, 735
MunI CAATTG 1 cut(s) 423
MvnI CGCG 1 cut(s) 771
MwoI GCNNNNNNNGC 6 cut(s) 200, 383, 575, 635, 744, 774
NciI CCSGG 2 cut(s) 24, 735
NcoI CCATGG 1 cut(s) 439
NlaIII CATG 3 cut(s) 374, 443, 594
NlaIV GGNNCC 1 cut(s) 31
NmeAIII GCCGAG 1 cut(s) 200
NspV TTCGAA 2 cut(s) 456, 462
PciSI GCTCTTC 1 cut(s) 101
PcsI WCGNNNNNNNCGW 3 cut(s) 171, 183, 336
PdmI GAANNNNTTC 1 cut(s) 468
PfeI GAWTC 6 cut(s) 120, 188, 322, 341, 432, 444
PkrI GCNGC 2 cut(s) 421, 770
PleI GAGTC 1 cut(s) 557
PpsI GAGTC 1 cut(s) 557
PspFI CCCAGC 1 cut(s) 113
PspN4I GGNNCC 1 cut(s) 31
PspPI GGNCC 1 cut(s) 594
RsaI GTAC 3 cut(s) 357, 666, 813
RsaNI GTAC 3 cut(s) 356, 665, 812
SacII CCGCGG 1 cut(s) 772
SapI GCTCTTC 1 cut(s) 101
SatI GCNGC 2 cut(s) 420, 769
Sau96I GGNCC 1 cut(s) 594
SchI GAGTC 1 cut(s) 558
ScrFI CCNGG 2 cut(s) 24, 735
SduI GDGCHC 4 cut(s) 12, 34, 686, 715
SetI ASST 6 cut(s) 22, 62, 416, 547, 806, 821
SfaNI GCATC 1 cut(s) 638
Sfr303I CCGCGG 1 cut(s) 772
SfuI TTCGAA 2 cut(s) 456, 462
SgrBI CCGCGG 1 cut(s) 772
Sse9I AATT 6 cut(s) 71, 423, 458, 603, 659, 785
SsiI CCGC 9 cut(s) 27, 46, 65, 92, 201, 260, 576, 769, 771
SspMI CTAG 2 cut(s) 5, 311
StyD4I CCNGG 2 cut(s) 22, 733
StyI CCWWGG 3 cut(s) 439, 564, 597
TaiI ACGT 3 cut(s) 416, 547, 806
TaqI TCGA 6 cut(s) 186, 270, 330, 456, 462, 632
TasI AATT 6 cut(s) 71, 423, 458, 603, 659, 785
TauI GCSGC 1 cut(s) 771
TfiI GAWTC 6 cut(s) 120, 188, 322, 341, 432, 444
TscAI CASTG 1 cut(s) 691
TseI GCWGC 1 cut(s) 419
TspDTI ATGAA 1 cut(s) 478
TspGWI ACGGA 2 cut(s) 192, 256
TspRI CASTG 1 cut(s) 691
XagI CCTNNNNNAGG 1 cut(s) 312
XapI RAATTY 4 cut(s) 71, 458, 603, 659
XbaI TCTAGA 1 cut(s) 310
XmnI GAANNNNTTC 1 cut(s) 468
XspI CTAG 2 cut(s) 5, 311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.