Rw6G042730

Protein of unknown function (DUF3054)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0011949
Physical Location & Seq
Forward (+)
66121745 .. 66125040
3296 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G042730.1

Sequence Viewer

Length: 813 bp
ATGCTAATTCTGAGCCAAGCTCCCACCGGAGCCTTATCGGCCAGCTGTTCTCGCAAGCCCCCTCTCAAATTCACCAGCCCGAACCCGCCAATCTTCTCGACCCGAAAACCCAAATTCCTTCCCAGACCCAACCCGTCCAAGTCCCTCAACCTCACGCTCGCTAAAGCCGTCGACGGAGGAGGAGGCGTCGATTCCACCTCACCCGCCGCCGCCGTCAAGCCGCTCCCCTCTCCTCCTCCGTCGCCGTTAGACAATGACCAGCCCGTCTTCGTCGGCGGCGAGAATATGCCCTTGGAAGGCGTCATTCAGTTCGACAAGCCCAGGCCCAGCTCCTCTGCTCGAATCAGAAAATGGGGTAAGGTGGCATTGCTCGCCGGCGGGGATGTTCTGGCTTTGCTGACGTTCGCTGCAATTGGGAGATTCAGTCATGGCTTCCCTGTTTTGGACTTCGAAACCCTCCGCACTGCTGACCCTTTTATTGCCGGTTGGTTTTTGAGTGCTTATTTCCTCGGAGGCTTTGGGGAAGACGGACGTGGAATCAATGGTCTGCCTAAGGCTGTTACTGCTTCTGCAAAGTCATGGGCCCTCGGAATCCCACTTGGGATAGTCATAAGGGCATCAACAGCAGGTCACATTCCCTCATTCAGTTTTGTGTTGGTATCAATGGGGAGCACTGCTGTTCTACTTTTGGGATGGAGGACGTTATTGTATAAATTCCTTCCTGGTGATAAGAGCAAAAAGAGTGATGTATATCGCCGTGGTAGCCCGTTTGAGTTGTTTGAGTTGCTCACATCTCTAGTACGAAGATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3054

Protein Analysis

270

Amino Acids

28.76

Weight (kDa)

10.22

Isoelectric Point (pI)

43.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3054 PF11255 126 - 235 7.7e-30 Protein of unknown function (DUF3054)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011949)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44920
fragaria_vesca FvH4_2g32650
malus_domestica MD08G1077300.v1.1 MD15G1064800.v1.1
prunus_persica Prupe.1G418700_v2.0.a1
pyrus_communis pycom08g06420 pycom15g06150
rosa_chinensis RchiOBHm_Chr6g0310081
rosa_laevigata RLG00000010513
rosa_multiflora Rmu_sc0000294.1_g000021 Rmu_sc0038452.1_g000001
rosa_roxburghii Rroxscaffold_7G00158900
rosa_rugosa Rorug06G0377200 Rorug06G0377200
rosa_samantha Rh6AG491100 Rh6BG500500 Rh6CG505400 Rh6DG491400
rosa_wichuraiana Rw6G042730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 263
Acc36I ACCTGC 1 cut(s) 617
AccBSI CCGCTC 1 cut(s) 223
AccI GTMKAC 1 cut(s) 171
AciI CCGC 8 cut(s) 86, 204, 207, 210, 221, 276, 378, 460
AcoI YGGCCR 1 cut(s) 39
AcsI RAATTY 3 cut(s) 68, 113, 713
AcyI GRCGYC 2 cut(s) 186, 300
AfaI GTAC 1 cut(s) 801
AfiI CCNNNNNNNGG 2 cut(s) 296, 442
AjiI CACGTC 1 cut(s) 533
AjnI CCWGG 2 cut(s) 320, 721
AjuI GAANNNNNNNTTGG 2 cut(s) 275, 307
AluBI AGCT 3 cut(s) 20, 45, 330
AluI AGCT 3 cut(s) 20, 45, 330
Alw21I GWGCWC 1 cut(s) 674
AoxI GGCC 3 cut(s) 39, 323, 582
ApaI GGGCCC 1 cut(s) 586
ApeKI GCWGC 1 cut(s) 407
ApoI RAATTY 3 cut(s) 68, 113, 713
AspS9I GGNCC 3 cut(s) 324, 582, 583
AsuHPI GGTGA 3 cut(s) 64, 192, 737
AsuII TTCGAA 1 cut(s) 450
AxyI CCTNAGG 1 cut(s) 552
BaeGI GKGCMC 1 cut(s) 586
BanII GRGCYC 1 cut(s) 586
BbsI GAAGAC 2 cut(s) 259, 531
Bbv12I GWGCWC 1 cut(s) 674
BbvI GCAGC 1 cut(s) 394
BccI CCATC 2 cut(s) 687, 801
BceAI ACGGC 4 cut(s) 152, 197, 229, 741
BciT130I CCWGG 2 cut(s) 322, 723
BfaI CTAG 1 cut(s) 797
BfuAI ACCTGC 1 cut(s) 617
BglI GCCNNNNNGGC 1 cut(s) 38
BisI GCNGC 5 cut(s) 207, 210, 221, 277, 408
BlsI GCNGC 5 cut(s) 208, 211, 222, 278, 409
Bme1390I CCNGG 2 cut(s) 322, 723
BmgBI CACGTC 1 cut(s) 533
BmgT120I GGNCC 3 cut(s) 324, 582, 583
BmiI GGNNCC 2 cut(s) 31, 584
BmrFI CCNGG 2 cut(s) 322, 723
BmsI GCATC 1 cut(s) 626
BpiI GAAGAC 2 cut(s) 259, 531
BplI GAGNNNNNCTC 1 cut(s) 36
Bpu14I TTCGAA 1 cut(s) 450
BsaBI GATNNNNATC 1 cut(s) 750
BsaHI GRCGYC 2 cut(s) 186, 300
BsaJI CCNNGG 5 cut(s) 291, 320, 508, 586, 757
BsaWI WCCGGW 1 cut(s) 26
BsaXI ACNNNNNCTCC 4 cut(s) 171, 201, 409, 439
Bsc4I CCNNNNNNNGG 2 cut(s) 296, 442
Bse118I RCCGGY 2 cut(s) 374, 482
Bse21I CCTNAGG 1 cut(s) 552
Bse3DI GCAATG 1 cut(s) 365
Bse8I GATNNNNATC 1 cut(s) 750
BseBI CCWGG 2 cut(s) 322, 723
BseDI CCNNGG 5 cut(s) 291, 320, 508, 586, 757
BseGI GGATG 2 cut(s) 388, 698
BseJI GATNNNNATC 1 cut(s) 750
BseLI CCNNNNNNNGG 2 cut(s) 296, 442
BseMI GCAATG 1 cut(s) 365
BseRI GAGGAG 5 cut(s) 192, 195, 222, 225, 322
BseSI GKGCMC 1 cut(s) 586
BseXI GCAGC 1 cut(s) 394
BseYI CCCAGC 1 cut(s) 326
BshFI GGCC 3 cut(s) 41, 325, 584
BsiHKAI GWGCWC 1 cut(s) 674
BsiSI CCGG 3 cut(s) 27, 375, 483
BslFI GGGAC 1 cut(s) 127
BslI CCNNNNNNNGG 2 cut(s) 296, 442
BsmFI GGGAC 1 cut(s) 127
BsnI GGCC 3 cut(s) 41, 325, 584
Bsp119I TTCGAA 1 cut(s) 450
Bsp120I GGGCCC 1 cut(s) 582
Bsp1286I GDGCHC 2 cut(s) 586, 674
BspACI CCGC 8 cut(s) 86, 204, 207, 210, 221, 276, 378, 460
BspANI GGCC 3 cut(s) 41, 325, 584
BspLI GGNNCC 2 cut(s) 31, 584
BspMI ACCTGC 1 cut(s) 617
BspT104I TTCGAA 1 cut(s) 450
BsrBI CCGCTC 1 cut(s) 223
BsrDI GCAATG 1 cut(s) 365
BsrFI RCCGGY 2 cut(s) 374, 482
BssAI RCCGGY 2 cut(s) 374, 482
BssECI CCNNGG 5 cut(s) 291, 320, 508, 586, 757
BssNI GRCGYC 2 cut(s) 186, 300
BssT1I CCWWGG 1 cut(s) 291
Bst2UI CCWGG 2 cut(s) 322, 723
BstACI GRCGYC 2 cut(s) 186, 300
BstBI TTCGAA 1 cut(s) 450
BstC8I GCNNGC 5 cut(s) 43, 56, 159, 372, 376
BstDEI CTNAG 2 cut(s) 11, 552
BstDSI CCRYGG 1 cut(s) 757
BstF5I GGATG 2 cut(s) 388, 698
BstMWI GCNNNNNNNGC 6 cut(s) 38, 51, 371, 563, 623, 762
BstNI CCWGG 2 cut(s) 322, 723
BstSCI CCNGG 2 cut(s) 320, 721
BstSLI GKGCMC 1 cut(s) 586
BstV1I GCAGC 1 cut(s) 394
BstV2I GAAGAC 2 cut(s) 259, 531
Bsu36I CCTNAGG 1 cut(s) 552
BsuRI GGCC 3 cut(s) 41, 325, 584
BtgI CCRYGG 1 cut(s) 757
BtrI CACGTC 1 cut(s) 533
BtsCI GGATG 2 cut(s) 388, 698
BtsI GCAGTG 2 cut(s) 462, 672
BtsIMutI CAGTG 2 cut(s) 462, 672
BveI ACCTGC 1 cut(s) 617
Cac8I GCNNGC 5 cut(s) 43, 56, 159, 372, 376
Cfr10I RCCGGY 2 cut(s) 374, 482
Cfr13I GGNCC 3 cut(s) 324, 582, 583
CseI GACGC 2 cut(s) 175, 289
Csp6I GTAC 1 cut(s) 800
CviAII CATG 2 cut(s) 428, 579
CviQI GTAC 1 cut(s) 800
DdeI CTNAG 2 cut(s) 11, 552
DrdI GACNNNNNNGTC 1 cut(s) 263
DseDI GACNNNNNNGTC 1 cut(s) 263
EaeI YGGCCR 1 cut(s) 39
Eco130I CCWWGG 1 cut(s) 291
Eco24I GRGCYC 1 cut(s) 586
Eco81I CCTNAGG 1 cut(s) 552
EcoO109I RGGNCCY 1 cut(s) 583
EcoRII CCWGG 2 cut(s) 320, 721
EcoT14I CCWWGG 1 cut(s) 291
EcoT38I GRGCYC 1 cut(s) 586
ErhI CCWWGG 1 cut(s) 291
FaeI CATG 2 cut(s) 431, 582
FaiI YATR 6 cut(s) 287, 429, 580, 611, 711, 751
FaqI GGGAC 1 cut(s) 127
FatI CATG 2 cut(s) 427, 578
FauI CCCGC 3 cut(s) 93, 211, 371
FblI GTMKAC 1 cut(s) 171
Fnu4HI GCNGC 5 cut(s) 207, 210, 221, 277, 408
FokI GGATG 2 cut(s) 395, 705
FriOI GRGCYC 1 cut(s) 586
Fsp4HI GCNGC 5 cut(s) 207, 210, 221, 277, 408
FspBI CTAG 1 cut(s) 797
GluI GCNGC 5 cut(s) 207, 210, 221, 277, 408
GsaI CCCAGC 1 cut(s) 330
HaeIII GGCC 3 cut(s) 41, 325, 584
HapII CCGG 3 cut(s) 27, 375, 483
HgaI GACGC 2 cut(s) 175, 289
Hin1I GRCGYC 2 cut(s) 186, 300
Hin1II CATG 2 cut(s) 431, 582
HincII GTYRAC 1 cut(s) 172
HindII GTYRAC 1 cut(s) 172
HinfI GANTC 5 cut(s) 191, 342, 420, 537, 591
HpaII CCGG 3 cut(s) 27, 375, 483
HphI GGTGA 3 cut(s) 64, 192, 737
Hpy166II GTNNAC 1 cut(s) 172
Hpy188I TCNGA 4 cut(s) 12, 347, 512, 590
Hpy188III TCNNGA 1 cut(s) 97
Hpy8I GTNNAC 1 cut(s) 172
Hpy99I CGWCG 5 cut(s) 173, 176, 191, 244, 275
HpyAV CCTTC 3 cut(s) 128, 290, 728
HpyCH4IV ACGT 3 cut(s) 401, 532, 701
HpyCH4V TGCA 2 cut(s) 410, 572
HpyF10VI GCNNNNNNNGC 6 cut(s) 38, 51, 371, 563, 623, 762
HpyF3I CTNAG 2 cut(s) 11, 552
HpySE526I ACGT 3 cut(s) 401, 532, 701
Hsp92I GRCGYC 2 cut(s) 186, 300
Hsp92II CATG 2 cut(s) 431, 582
KroI GCCGGC 1 cut(s) 374
KroNI GCCGGC 1 cut(s) 376
LmnI GCTCC 5 cut(s) 25, 29, 228, 335, 669
Lsp1109I GCAGC 1 cut(s) 394
LweI GCATC 1 cut(s) 626
MaeI CTAG 1 cut(s) 797
MaeII ACGT 3 cut(s) 401, 532, 701
MaeIII GTNAC 2 cut(s) 559, 629
MbiI CCGCTC 1 cut(s) 223
MboII GAAGA 3 cut(s) 85, 259, 536
MfeI CAATTG 1 cut(s) 411
MhlI GDGCHC 2 cut(s) 586, 674
MluCI AATT 5 cut(s) 6, 68, 113, 411, 713
MreI CGCCGGCG 1 cut(s) 374
MroNI GCCGGC 1 cut(s) 374
MspA1I CMGCKG 1 cut(s) 45
MspI CCGG 3 cut(s) 27, 375, 483
MspR9I CCNGG 2 cut(s) 322, 723
MunI CAATTG 1 cut(s) 411
MvaI CCWGG 2 cut(s) 322, 723
MwoI GCNNNNNNNGC 6 cut(s) 38, 51, 371, 563, 623, 762
NaeI GCCGGC 1 cut(s) 376
NgoMIV GCCGGC 1 cut(s) 374
NlaIII CATG 2 cut(s) 431, 582
NlaIV GGNNCC 2 cut(s) 31, 584
NmuCI GTSAC 1 cut(s) 629
NspV TTCGAA 1 cut(s) 450
PcsI WCGNNNNNNNCGW 2 cut(s) 165, 276
PdiI GCCGGC 1 cut(s) 376
PfeI GAWTC 5 cut(s) 191, 342, 420, 537, 591
PkrI GCNGC 5 cut(s) 208, 211, 222, 278, 409
Psp6I CCWGG 2 cut(s) 320, 721
PspFI CCCAGC 1 cut(s) 326
PspGI CCWGG 2 cut(s) 320, 721
PspN4I GGNNCC 2 cut(s) 31, 584
PspOMI GGGCCC 1 cut(s) 582
PspPI GGNCC 3 cut(s) 324, 582, 583
PvuII CAGCTG 1 cut(s) 45
RsaI GTAC 1 cut(s) 801
RsaNI GTAC 1 cut(s) 800
SalI GTCGAC 1 cut(s) 170
SatI GCNGC 5 cut(s) 207, 210, 221, 277, 408
Sau96I GGNCC 3 cut(s) 324, 582, 583
ScrFI CCNGG 2 cut(s) 322, 723
SduI GDGCHC 2 cut(s) 586, 674
SfaNI GCATC 1 cut(s) 626
SfuI TTCGAA 1 cut(s) 450
SgrAI CRCCGGYG 1 cut(s) 374
SgrDI CGTCGACG 1 cut(s) 170
Sse9I AATT 5 cut(s) 6, 68, 113, 411, 713
SsiI CCGC 8 cut(s) 86, 204, 207, 210, 221, 276, 378, 460
SspMI CTAG 1 cut(s) 797
StyD4I CCNGG 2 cut(s) 320, 721
StyI CCWWGG 1 cut(s) 291
TaiI ACGT 3 cut(s) 404, 535, 704
TaqI TCGA 6 cut(s) 98, 171, 189, 312, 340, 450
TasI AATT 5 cut(s) 6, 68, 113, 411, 713
TauI GCSGC 4 cut(s) 209, 212, 223, 279
TfiI GAWTC 5 cut(s) 191, 342, 420, 537, 591
TscAI CASTG 2 cut(s) 469, 679
TseFI GTSAC 1 cut(s) 629
TseI GCWGC 1 cut(s) 407
Tsp45I GTSAC 1 cut(s) 629
TspGWI ACGGA 3 cut(s) 189, 228, 543
TspRI CASTG 2 cut(s) 469, 679
XapI RAATTY 3 cut(s) 68, 113, 713
XmiI GTMKAC 1 cut(s) 171
XspI CTAG 1 cut(s) 797
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.