Rroxscaffold_7G00158900

Protein of unknown function (DUF3054)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0011949
Physical Location & Seq
Reverse (-)
2238664 .. 2240696
2033 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00158900.1

Sequence Viewer

Length: 816 bp
ATGCTAATTCTGAGCCAAGCTCCCACCGGAGCCTTATCGGCCAGCTGTTCTCGCAAGCCCCCTCTCAAATTCACCAACCCGAACCCGCCAATCTTCTCGACCCGAAAACCCAAATTCCTTTCCAGACCCAACCCGTCCAAGTCCCTCAACCTCACGCTCGCTAAAGCCGTGGACGGAGGAGGAGGCGTCGATTCCACCTCACCCGCCGCCGTCAAGCCGCTCCCCAATTCCTCCGCCTCTCCTCCTCCGTCGCCGTTAGACAATGACCAGCCCGTCTTCGTCGGCGGCGAGAATATGCCCTTGGAAGGCGTCATTCAGTTCGACAAGCCCAGCTCCTCTGCTCGAATCAGAAAATGGGGTAAGGTGGCATTGCTCGCCGGCGGGGATGTTCTGGCTTTGCTGACGTTCGCTGCAATTGGGAGATTCAGTCATGGCTTCCCTGTTTTGGACTTTGAAACCCTCCGCACTGCTGACCCTTTTATTGCCGGTTGGTTTTTGAGTGCTTATTTCCTCGGAGGCTTTGGGGAAGACGGACGTGGAATCAATGGTCTGCCTAAGGCTGTTACTGCTTCTGCTAAGTCATGGGCCCTCGGAATCCCACTTGGGATTGTCATAAGGGCATCAACAGCAGGTCACATTCCCTCATTCAGTTTTGTGTTGGTATCAATGGGGAGCACTGCTGTTCTACTTTTGGGATGGAGGACGTTATTGTATAAATTCCTTCCTGGTGATAAGAGCAAAAAGAGTGATGTATATCGCCGCGGTAGCCCGTTTGAGTTGTTTGAGTTGCTCACATCTCTAGTACGAAGATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3054

Protein Analysis

271

Amino Acids

28.81

Weight (kDa)

10.13

Isoelectric Point (pI)

40.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3054 PF11255 127 - 236 7.8e-30 Protein of unknown function (DUF3054)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011949)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44920
fragaria_vesca FvH4_2g32650
malus_domestica MD08G1077300.v1.1 MD15G1064800.v1.1
prunus_persica Prupe.1G418700_v2.0.a1
pyrus_communis pycom08g06420 pycom15g06150
rosa_chinensis RchiOBHm_Chr6g0310081
rosa_laevigata RLG00000010513
rosa_multiflora Rmu_sc0000294.1_g000021 Rmu_sc0038452.1_g000001
rosa_roxburghii Rroxscaffold_7G00158900
rosa_rugosa Rorug06G0377200 Rorug06G0377200
rosa_samantha Rh6AG491100 Rh6BG500500 Rh6CG505400 Rh6DG491400
rosa_wichuraiana Rw6G042730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 272
Acc36I ACCTGC 1 cut(s) 620
AccBSI CCGCTC 1 cut(s) 220
AccII CGCG 1 cut(s) 762
AcoI YGGCCR 1 cut(s) 39
AcsI RAATTY 3 cut(s) 68, 113, 716
AcyI GRCGYC 2 cut(s) 186, 309
AfaI GTAC 1 cut(s) 804
AfiI CCNNNNNNNGG 2 cut(s) 305, 445
AgsI TTSAA 1 cut(s) 455
AjiI CACGTC 1 cut(s) 536
AjnI CCWGG 1 cut(s) 724
AjuI GAANNNNNNNTTGG 2 cut(s) 284, 316
AluBI AGCT 3 cut(s) 20, 45, 333
AluI AGCT 3 cut(s) 20, 45, 333
Alw21I GWGCWC 1 cut(s) 677
AoxI GGCC 2 cut(s) 39, 585
ApaI GGGCCC 1 cut(s) 589
ApeKI GCWGC 1 cut(s) 410
ApoI RAATTY 3 cut(s) 68, 113, 716
AspS9I GGNCC 2 cut(s) 585, 586
AsuHPI GGTGA 3 cut(s) 64, 192, 740
AxyI CCTNAGG 1 cut(s) 555
BaeGI GKGCMC 1 cut(s) 589
BanII GRGCYC 1 cut(s) 589
BbsI GAAGAC 2 cut(s) 268, 534
Bbv12I GWGCWC 1 cut(s) 677
BbvI GCAGC 1 cut(s) 397
BccI CCATC 2 cut(s) 690, 804
BceAI ACGGC 3 cut(s) 152, 194, 238
BciT130I CCWGG 1 cut(s) 726
BfaI CTAG 1 cut(s) 800
BfuAI ACCTGC 1 cut(s) 620
BglI GCCNNNNNGGC 1 cut(s) 38
BisI GCNGC 5 cut(s) 207, 218, 286, 411, 760
BlsI GCNGC 5 cut(s) 208, 219, 287, 412, 761
Bme1390I CCNGG 1 cut(s) 726
BmgBI CACGTC 1 cut(s) 536
BmgT120I GGNCC 2 cut(s) 585, 586
BmiI GGNNCC 2 cut(s) 31, 587
BmrFI CCNGG 1 cut(s) 726
BmsI GCATC 1 cut(s) 629
BpiI GAAGAC 2 cut(s) 268, 534
BplI GAGNNNNNCTC 1 cut(s) 36
BsaBI GATNNNNATC 1 cut(s) 753
BsaHI GRCGYC 2 cut(s) 186, 309
BsaJI CCNNGG 5 cut(s) 168, 300, 511, 589, 760
BsaWI WCCGGW 1 cut(s) 26
BsaXI ACNNNNNCTCC 4 cut(s) 171, 201, 412, 442
Bsc4I CCNNNNNNNGG 2 cut(s) 305, 445
Bse118I RCCGGY 2 cut(s) 377, 485
Bse21I CCTNAGG 1 cut(s) 555
Bse3DI GCAATG 1 cut(s) 368
Bse8I GATNNNNATC 1 cut(s) 753
BseBI CCWGG 1 cut(s) 726
BseDI CCNNGG 5 cut(s) 168, 300, 511, 589, 760
BseGI GGATG 2 cut(s) 391, 701
BseJI GATNNNNATC 1 cut(s) 753
BseLI CCNNNNNNNGG 2 cut(s) 305, 445
BseMI GCAATG 1 cut(s) 368
BseRI GAGGAG 5 cut(s) 192, 195, 231, 234, 325
BseSI GKGCMC 1 cut(s) 589
BseXI GCAGC 1 cut(s) 397
BseYI CCCAGC 1 cut(s) 329
Bsh1236I CGCG 1 cut(s) 762
BshFI GGCC 2 cut(s) 41, 587
BsiHKAI GWGCWC 1 cut(s) 677
BsiSI CCGG 3 cut(s) 27, 378, 486
BslFI GGGAC 1 cut(s) 127
BslI CCNNNNNNNGG 2 cut(s) 305, 445
BsmFI GGGAC 1 cut(s) 127
BsnI GGCC 2 cut(s) 41, 587
Bsp120I GGGCCC 1 cut(s) 585
Bsp1286I GDGCHC 2 cut(s) 589, 677
BspANI GGCC 2 cut(s) 41, 587
BspFNI CGCG 1 cut(s) 762
BspLI GGNNCC 2 cut(s) 31, 587
BspMI ACCTGC 1 cut(s) 620
BsrBI CCGCTC 1 cut(s) 220
BsrDI GCAATG 1 cut(s) 368
BsrFI RCCGGY 2 cut(s) 377, 485
BssAI RCCGGY 2 cut(s) 377, 485
BssECI CCNNGG 5 cut(s) 168, 300, 511, 589, 760
BssNI GRCGYC 2 cut(s) 186, 309
BssT1I CCWWGG 1 cut(s) 300
Bst2UI CCWGG 1 cut(s) 726
BstACI GRCGYC 2 cut(s) 186, 309
BstC8I GCNNGC 5 cut(s) 43, 56, 159, 375, 379
BstDEI CTNAG 3 cut(s) 11, 555, 576
BstDSI CCRYGG 2 cut(s) 168, 760
BstF5I GGATG 2 cut(s) 391, 701
BstFNI CGCG 1 cut(s) 762
BstMWI GCNNNNNNNGC 6 cut(s) 38, 51, 374, 566, 626, 765
BstNI CCWGG 1 cut(s) 726
BstSCI CCNGG 1 cut(s) 724
BstSLI GKGCMC 1 cut(s) 589
BstUI CGCG 1 cut(s) 762
BstV1I GCAGC 1 cut(s) 397
BstV2I GAAGAC 2 cut(s) 268, 534
Bsu36I CCTNAGG 1 cut(s) 555
BsuRI GGCC 2 cut(s) 41, 587
BtgI CCRYGG 2 cut(s) 168, 760
BtrI CACGTC 1 cut(s) 536
BtsCI GGATG 2 cut(s) 391, 701
BtsI GCAGTG 2 cut(s) 465, 675
BtsIMutI CAGTG 2 cut(s) 465, 675
BveI ACCTGC 1 cut(s) 620
Cac8I GCNNGC 5 cut(s) 43, 56, 159, 375, 379
Cfr10I RCCGGY 2 cut(s) 377, 485
Cfr13I GGNCC 2 cut(s) 585, 586
Cfr42I CCGCGG 1 cut(s) 763
CseI GACGC 2 cut(s) 175, 298
Csp6I GTAC 1 cut(s) 803
CviAII CATG 2 cut(s) 431, 582
CviQI GTAC 1 cut(s) 803
DdeI CTNAG 3 cut(s) 11, 555, 576
DrdI GACNNNNNNGTC 1 cut(s) 272
DseDI GACNNNNNNGTC 1 cut(s) 272
EaeI YGGCCR 1 cut(s) 39
EciI GGCGGA 1 cut(s) 223
Eco130I CCWWGG 1 cut(s) 300
Eco24I GRGCYC 1 cut(s) 589
Eco81I CCTNAGG 1 cut(s) 555
EcoO109I RGGNCCY 1 cut(s) 586
EcoRII CCWGG 1 cut(s) 724
EcoT14I CCWWGG 1 cut(s) 300
EcoT38I GRGCYC 1 cut(s) 589
ErhI CCWWGG 1 cut(s) 300
FaeI CATG 2 cut(s) 434, 585
FaiI YATR 6 cut(s) 296, 432, 583, 614, 714, 754
FaqI GGGAC 1 cut(s) 127
FatI CATG 2 cut(s) 430, 581
FauI CCCGC 3 cut(s) 93, 211, 374
Fnu4HI GCNGC 5 cut(s) 207, 218, 286, 411, 760
FokI GGATG 2 cut(s) 398, 708
FriOI GRGCYC 1 cut(s) 589
Fsp4HI GCNGC 5 cut(s) 207, 218, 286, 411, 760
FspBI CTAG 1 cut(s) 800
GluI GCNGC 5 cut(s) 207, 218, 286, 411, 760
GsaI CCCAGC 1 cut(s) 333
HaeIII GGCC 2 cut(s) 41, 587
HapII CCGG 3 cut(s) 27, 378, 486
HgaI GACGC 2 cut(s) 175, 298
Hin1I GRCGYC 2 cut(s) 186, 309
Hin1II CATG 2 cut(s) 434, 585
HinfI GANTC 5 cut(s) 191, 345, 423, 540, 594
HpaII CCGG 3 cut(s) 27, 378, 486
HphI GGTGA 3 cut(s) 64, 192, 740
Hpy166II GTNNAC 1 cut(s) 172
Hpy188I TCNGA 4 cut(s) 12, 350, 515, 593
Hpy188III TCNNGA 2 cut(s) 97, 123
Hpy8I GTNNAC 1 cut(s) 172
Hpy99I CGWCG 3 cut(s) 191, 253, 284
HpyAV CCTTC 2 cut(s) 299, 731
HpyCH4IV ACGT 3 cut(s) 404, 535, 704
HpyCH4V TGCA 1 cut(s) 413
HpyF10VI GCNNNNNNNGC 6 cut(s) 38, 51, 374, 566, 626, 765
HpyF3I CTNAG 3 cut(s) 11, 555, 576
HpySE526I ACGT 3 cut(s) 404, 535, 704
Hsp92I GRCGYC 2 cut(s) 186, 309
Hsp92II CATG 2 cut(s) 434, 585
KroI GCCGGC 1 cut(s) 377
KroNI GCCGGC 1 cut(s) 379
KspI CCGCGG 1 cut(s) 763
LmnI GCTCC 5 cut(s) 25, 29, 225, 338, 672
Lsp1109I GCAGC 1 cut(s) 397
LweI GCATC 1 cut(s) 629
MaeI CTAG 1 cut(s) 800
MaeII ACGT 3 cut(s) 404, 535, 704
MaeIII GTNAC 2 cut(s) 562, 632
MbiI CCGCTC 1 cut(s) 220
MboII GAAGA 3 cut(s) 85, 268, 539
MfeI CAATTG 1 cut(s) 414
MhlI GDGCHC 2 cut(s) 589, 677
MluCI AATT 6 cut(s) 6, 68, 113, 226, 414, 716
MreI CGCCGGCG 1 cut(s) 377
MroNI GCCGGC 1 cut(s) 377
MspA1I CMGCKG 2 cut(s) 45, 762
MspI CCGG 3 cut(s) 27, 378, 486
MspR9I CCNGG 1 cut(s) 726
MunI CAATTG 1 cut(s) 414
MvaI CCWGG 1 cut(s) 726
MvnI CGCG 1 cut(s) 762
MwoI GCNNNNNNNGC 6 cut(s) 38, 51, 374, 566, 626, 765
NaeI GCCGGC 1 cut(s) 379
NgoMIV GCCGGC 1 cut(s) 377
NlaIII CATG 2 cut(s) 434, 585
NlaIV GGNNCC 2 cut(s) 31, 587
NmuCI GTSAC 1 cut(s) 632
PcsI WCGNNNNNNNCGW 2 cut(s) 165, 285
PdiI GCCGGC 1 cut(s) 379
PfeI GAWTC 5 cut(s) 191, 345, 423, 540, 594
PkrI GCNGC 5 cut(s) 208, 219, 287, 412, 761
Psp6I CCWGG 1 cut(s) 724
PspFI CCCAGC 1 cut(s) 329
PspGI CCWGG 1 cut(s) 724
PspN4I GGNNCC 2 cut(s) 31, 587
PspOMI GGGCCC 1 cut(s) 585
PspPI GGNCC 2 cut(s) 585, 586
PvuII CAGCTG 1 cut(s) 45
RsaI GTAC 1 cut(s) 804
RsaNI GTAC 1 cut(s) 803
SacII CCGCGG 1 cut(s) 763
SatI GCNGC 5 cut(s) 207, 218, 286, 411, 760
Sau96I GGNCC 2 cut(s) 585, 586
ScrFI CCNGG 1 cut(s) 726
SduI GDGCHC 2 cut(s) 589, 677
SfaNI GCATC 1 cut(s) 629
Sfr303I CCGCGG 1 cut(s) 763
SgrAI CRCCGGYG 1 cut(s) 377
SgrBI CCGCGG 1 cut(s) 763
Sse9I AATT 6 cut(s) 6, 68, 113, 226, 414, 716
SspMI CTAG 1 cut(s) 800
StyD4I CCNGG 1 cut(s) 724
StyI CCWWGG 1 cut(s) 300
TaiI ACGT 3 cut(s) 407, 538, 707
TaqI TCGA 4 cut(s) 98, 189, 321, 343
TasI AATT 6 cut(s) 6, 68, 113, 226, 414, 716
TauI GCSGC 4 cut(s) 209, 220, 288, 762
TfiI GAWTC 5 cut(s) 191, 345, 423, 540, 594
TscAI CASTG 2 cut(s) 472, 682
TseFI GTSAC 1 cut(s) 632
TseI GCWGC 1 cut(s) 410
Tsp45I GTSAC 1 cut(s) 632
TspGWI ACGGA 3 cut(s) 189, 237, 546
TspRI CASTG 2 cut(s) 472, 682
XapI RAATTY 3 cut(s) 68, 113, 716
XspI CTAG 1 cut(s) 800
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.