RLG00000010513

Protein of unknown function (DUF3054)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0011949
Physical Location & Seq
Reverse (-)
2281928 .. 2283673
1746 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010513

Sequence Viewer

Length: 822 bp
ATGCTAATTCTGAGCCAAGCTCCCACCGGAGCCTTATCGGCCAGCTGTTCTCGCAAGCCCCCTCTCAAATTCACCAACCCGAACCCGCCAATCTTCTCGACCCGAAAACCCAAATTCGTATCCAGACCCAACCCGTCCAAGTCCCTCAACCTCACGCTCGCTAAAGCCGTCGACGGAGGAGGAGGCGTCGATTCCACCTCACCGGCCGCCGTCAAGCCGCTCCCCAATTCCTCCGCCTCTCCTCCTCCTCCTCCGTCGCCGTTAGACAATGACCAGCCCGTCTTCGTCGGCGGCGAGAATATGCCCTTGGAAGGCGTCATTCAGTTCGACAAGCCCAGCTCCTCTGCTCGAATCAGAAAATGGGGTAAGGTGGCATTGCTCGCCGGCGGGGATGTTCTGGCTTTGCTCACGTTCGCTGCAATTGGGAGATTCAGTCATGGCTTCCCTGTTTTGGACTTTGAAACCCTCCGCACTGCTGACCCTTTTATTGCCGGTTGGTTTTTGAGTGCTTATTTCCTCGGAGGCTTTGGGGAAGACGGACGTGGAATCAATGGTCTGCCTAAGGCTGTTTTTGCTTCTGCTAAGTCATGGGCCCTCGGAATCCCACTTGGGATAGTCATAAGGGCATCAACATCAGGTCACATTCCCTCATTCAGTTTTGTGTTGGTATCAATGGGGAGCACTGCTGTTCTACTTTTGGGATGGAGGACGTTATTGTATAAATTCCTTCCTGGTGATAAGAGCAAAAAGAGTGATGTATATCGCCGCGGTAGCCCGTTTGAGTTGTTTGAGTTGCTCACATCTCTAGTACGAAGATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3054

Protein Analysis

274

Amino Acids

29.05

Weight (kDa)

10.13

Isoelectric Point (pI)

42.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3054 PF11255 129 - 238 6.2e-30 Protein of unknown function (DUF3054)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011949)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44920
fragaria_vesca FvH4_2g32650
malus_domestica MD08G1077300.v1.1 MD15G1064800.v1.1
prunus_persica Prupe.1G418700_v2.0.a1
pyrus_communis pycom08g06420 pycom15g06150
rosa_chinensis RchiOBHm_Chr6g0310081
rosa_laevigata RLG00000010513
rosa_multiflora Rmu_sc0000294.1_g000021 Rmu_sc0038452.1_g000001
rosa_roxburghii Rroxscaffold_7G00158900
rosa_rugosa Rorug06G0377200 Rorug06G0377200
rosa_samantha Rh6AG491100 Rh6BG500500 Rh6CG505400 Rh6DG491400
rosa_wichuraiana Rw6G042730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 278
AccBSI CCGCTC 1 cut(s) 220
AccI GTMKAC 1 cut(s) 171
AccII CGCG 1 cut(s) 768
AciI CCGC 9 cut(s) 86, 207, 218, 234, 291, 387, 469, 766, 768
AcoI YGGCCR 2 cut(s) 39, 204
AcsI RAATTY 3 cut(s) 68, 113, 722
AcyI GRCGYC 2 cut(s) 186, 315
AfaI GTAC 1 cut(s) 810
AfiI CCNNNNNNNGG 2 cut(s) 311, 451
AgsI TTSAA 1 cut(s) 461
AjiI CACGTC 1 cut(s) 542
AjnI CCWGG 1 cut(s) 730
AjuI GAANNNNNNNTTGG 2 cut(s) 290, 322
AluBI AGCT 3 cut(s) 20, 45, 339
AluI AGCT 3 cut(s) 20, 45, 339
Alw21I GWGCWC 1 cut(s) 683
AoxI GGCC 3 cut(s) 39, 204, 591
ApaI GGGCCC 1 cut(s) 595
ApeKI GCWGC 1 cut(s) 416
ApoI RAATTY 3 cut(s) 68, 113, 722
AspS9I GGNCC 2 cut(s) 591, 592
AsuHPI GGTGA 3 cut(s) 64, 192, 746
AxyI CCTNAGG 1 cut(s) 561
BaeGI GKGCMC 1 cut(s) 595
BanII GRGCYC 1 cut(s) 595
BbsI GAAGAC 2 cut(s) 274, 540
Bbv12I GWGCWC 1 cut(s) 683
BbvI GCAGC 1 cut(s) 403
BccI CCATC 2 cut(s) 696, 810
BceAI ACGGC 3 cut(s) 152, 194, 244
BciT130I CCWGG 1 cut(s) 732
BciVI GTATCC 1 cut(s) 130
BfaI CTAG 1 cut(s) 806
BfuI GTATCC 1 cut(s) 130
BglI GCCNNNNNGGC 1 cut(s) 38
BisI GCNGC 5 cut(s) 207, 218, 292, 417, 766
BlsI GCNGC 5 cut(s) 208, 219, 293, 418, 767
Bme1390I CCNGG 1 cut(s) 732
BmgBI CACGTC 1 cut(s) 542
BmgT120I GGNCC 2 cut(s) 591, 592
BmiI GGNNCC 2 cut(s) 31, 593
BmrFI CCNGG 1 cut(s) 732
BmsI GCATC 1 cut(s) 635
BpiI GAAGAC 2 cut(s) 274, 540
BplI GAGNNNNNCTC 1 cut(s) 36
BsaBI GATNNNNATC 1 cut(s) 759
BsaHI GRCGYC 2 cut(s) 186, 315
BsaJI CCNNGG 4 cut(s) 306, 517, 595, 766
BsaWI WCCGGW 1 cut(s) 26
BsaXI ACNNNNNCTCC 4 cut(s) 171, 201, 418, 448
Bsc4I CCNNNNNNNGG 2 cut(s) 311, 451
Bse118I RCCGGY 3 cut(s) 202, 383, 491
Bse21I CCTNAGG 1 cut(s) 561
Bse3DI GCAATG 1 cut(s) 374
Bse8I GATNNNNATC 1 cut(s) 759
BseBI CCWGG 1 cut(s) 732
BseDI CCNNGG 4 cut(s) 306, 517, 595, 766
BseGI GGATG 2 cut(s) 397, 707
BseJI GATNNNNATC 1 cut(s) 759
BseLI CCNNNNNNNGG 2 cut(s) 311, 451
BseMI GCAATG 1 cut(s) 374
BseRI GAGGAG 7 cut(s) 192, 195, 231, 234, 237, 240, 331
BseSI GKGCMC 1 cut(s) 595
BseX3I CGGCCG 1 cut(s) 204
BseXI GCAGC 1 cut(s) 403
BseYI CCCAGC 1 cut(s) 335
Bsh1236I CGCG 1 cut(s) 768
Bsh1285I CGRYCG 1 cut(s) 207
BshFI GGCC 3 cut(s) 41, 206, 593
BsiEI CGRYCG 1 cut(s) 207
BsiHKAI GWGCWC 1 cut(s) 683
BsiSI CCGG 4 cut(s) 27, 203, 384, 492
BslFI GGGAC 1 cut(s) 127
BslI CCNNNNNNNGG 2 cut(s) 311, 451
BsmFI GGGAC 1 cut(s) 127
BsnI GGCC 3 cut(s) 41, 206, 593
Bsp120I GGGCCC 1 cut(s) 591
Bsp1286I GDGCHC 2 cut(s) 595, 683
BspACI CCGC 9 cut(s) 86, 207, 218, 234, 291, 387, 469, 766, 768
BspANI GGCC 3 cut(s) 41, 206, 593
BspFNI CGCG 1 cut(s) 768
BspLI GGNNCC 2 cut(s) 31, 593
BsrBI CCGCTC 1 cut(s) 220
BsrDI GCAATG 1 cut(s) 374
BsrFI RCCGGY 3 cut(s) 202, 383, 491
BssAI RCCGGY 3 cut(s) 202, 383, 491
BssECI CCNNGG 4 cut(s) 306, 517, 595, 766
BssNI GRCGYC 2 cut(s) 186, 315
BssT1I CCWWGG 1 cut(s) 306
Bst2UI CCWGG 1 cut(s) 732
BstACI GRCGYC 2 cut(s) 186, 315
BstC8I GCNNGC 5 cut(s) 43, 56, 159, 381, 385
BstDEI CTNAG 3 cut(s) 11, 561, 582
BstDSI CCRYGG 1 cut(s) 766
BstF5I GGATG 2 cut(s) 397, 707
BstFNI CGCG 1 cut(s) 768
BstMCI CGRYCG 1 cut(s) 207
BstMWI GCNNNNNNNGC 5 cut(s) 38, 51, 380, 572, 771
BstNI CCWGG 1 cut(s) 732
BstSCI CCNGG 1 cut(s) 730
BstSLI GKGCMC 1 cut(s) 595
BstUI CGCG 1 cut(s) 768
BstV1I GCAGC 1 cut(s) 403
BstV2I GAAGAC 2 cut(s) 274, 540
BstZI CGGCCG 1 cut(s) 204
Bsu36I CCTNAGG 1 cut(s) 561
BsuI GTATCC 1 cut(s) 130
BsuRI GGCC 3 cut(s) 41, 206, 593
BtgI CCRYGG 1 cut(s) 766
BtrI CACGTC 1 cut(s) 542
BtsCI GGATG 2 cut(s) 397, 707
BtsI GCAGTG 2 cut(s) 471, 681
BtsIMutI CAGTG 2 cut(s) 471, 681
Cac8I GCNNGC 5 cut(s) 43, 56, 159, 381, 385
Cfr10I RCCGGY 3 cut(s) 202, 383, 491
Cfr13I GGNCC 2 cut(s) 591, 592
Cfr42I CCGCGG 1 cut(s) 769
CseI GACGC 2 cut(s) 175, 304
Csp6I GTAC 1 cut(s) 809
CviAII CATG 2 cut(s) 437, 588
CviQI GTAC 1 cut(s) 809
DdeI CTNAG 3 cut(s) 11, 561, 582
DrdI GACNNNNNNGTC 1 cut(s) 278
DseDI GACNNNNNNGTC 1 cut(s) 278
EaeI YGGCCR 2 cut(s) 39, 204
EagI CGGCCG 1 cut(s) 204
EciI GGCGGA 1 cut(s) 223
EclXI CGGCCG 1 cut(s) 204
Eco130I CCWWGG 1 cut(s) 306
Eco24I GRGCYC 1 cut(s) 595
Eco52I CGGCCG 1 cut(s) 204
Eco81I CCTNAGG 1 cut(s) 561
EcoO109I RGGNCCY 1 cut(s) 592
EcoRII CCWGG 1 cut(s) 730
EcoT14I CCWWGG 1 cut(s) 306
EcoT38I GRGCYC 1 cut(s) 595
ErhI CCWWGG 1 cut(s) 306
FaeI CATG 2 cut(s) 440, 591
FaiI YATR 6 cut(s) 302, 438, 589, 620, 720, 760
FaqI GGGAC 1 cut(s) 127
FatI CATG 2 cut(s) 436, 587
FauI CCCGC 2 cut(s) 93, 380
FblI GTMKAC 1 cut(s) 171
Fnu4HI GCNGC 5 cut(s) 207, 218, 292, 417, 766
FokI GGATG 2 cut(s) 404, 714
FriOI GRGCYC 1 cut(s) 595
Fsp4HI GCNGC 5 cut(s) 207, 218, 292, 417, 766
FspBI CTAG 1 cut(s) 806
GluI GCNGC 5 cut(s) 207, 218, 292, 417, 766
GsaI CCCAGC 1 cut(s) 339
HaeIII GGCC 3 cut(s) 41, 206, 593
HapII CCGG 4 cut(s) 27, 203, 384, 492
HgaI GACGC 2 cut(s) 175, 304
Hin1I GRCGYC 2 cut(s) 186, 315
Hin1II CATG 2 cut(s) 440, 591
HincII GTYRAC 1 cut(s) 172
HindII GTYRAC 1 cut(s) 172
HinfI GANTC 5 cut(s) 191, 351, 429, 546, 600
HpaII CCGG 4 cut(s) 27, 203, 384, 492
HphI GGTGA 3 cut(s) 64, 192, 746
Hpy166II GTNNAC 1 cut(s) 172
Hpy188I TCNGA 4 cut(s) 12, 356, 521, 599
Hpy188III TCNNGA 2 cut(s) 97, 123
Hpy8I GTNNAC 1 cut(s) 172
Hpy99I CGWCG 5 cut(s) 173, 176, 191, 259, 290
HpyAV CCTTC 2 cut(s) 305, 737
HpyCH4IV ACGT 3 cut(s) 410, 541, 710
HpyCH4V TGCA 1 cut(s) 419
HpyF10VI GCNNNNNNNGC 5 cut(s) 38, 51, 380, 572, 771
HpyF3I CTNAG 3 cut(s) 11, 561, 582
HpySE526I ACGT 3 cut(s) 410, 541, 710
Hsp92I GRCGYC 2 cut(s) 186, 315
Hsp92II CATG 2 cut(s) 440, 591
KroI GCCGGC 1 cut(s) 383
KroNI GCCGGC 1 cut(s) 385
KspI CCGCGG 1 cut(s) 769
LmnI GCTCC 5 cut(s) 25, 29, 225, 344, 678
Lsp1109I GCAGC 1 cut(s) 403
LweI GCATC 1 cut(s) 635
MaeI CTAG 1 cut(s) 806
MaeII ACGT 3 cut(s) 410, 541, 710
MaeIII GTNAC 1 cut(s) 638
MbiI CCGCTC 1 cut(s) 220
MboII GAAGA 3 cut(s) 85, 274, 545
MfeI CAATTG 1 cut(s) 420
MhlI GDGCHC 2 cut(s) 595, 683
MluCI AATT 6 cut(s) 6, 68, 113, 226, 420, 722
MreI CGCCGGCG 1 cut(s) 383
MroNI GCCGGC 1 cut(s) 383
MspA1I CMGCKG 2 cut(s) 45, 768
MspI CCGG 4 cut(s) 27, 203, 384, 492
MspR9I CCNGG 1 cut(s) 732
MunI CAATTG 1 cut(s) 420
MvaI CCWGG 1 cut(s) 732
MvnI CGCG 1 cut(s) 768
MwoI GCNNNNNNNGC 5 cut(s) 38, 51, 380, 572, 771
NaeI GCCGGC 1 cut(s) 385
NgoMIV GCCGGC 1 cut(s) 383
NlaIII CATG 2 cut(s) 440, 591
NlaIV GGNNCC 2 cut(s) 31, 593
NmuCI GTSAC 1 cut(s) 638
PcsI WCGNNNNNNNCGW 2 cut(s) 165, 291
PdiI GCCGGC 1 cut(s) 385
PfeI GAWTC 5 cut(s) 191, 351, 429, 546, 600
PkrI GCNGC 5 cut(s) 208, 219, 293, 418, 767
Psp6I CCWGG 1 cut(s) 730
PspFI CCCAGC 1 cut(s) 335
PspGI CCWGG 1 cut(s) 730
PspN4I GGNNCC 2 cut(s) 31, 593
PspOMI GGGCCC 1 cut(s) 591
PspPI GGNCC 2 cut(s) 591, 592
PvuII CAGCTG 1 cut(s) 45
RsaI GTAC 1 cut(s) 810
RsaNI GTAC 1 cut(s) 809
SacII CCGCGG 1 cut(s) 769
SalI GTCGAC 1 cut(s) 170
SatI GCNGC 5 cut(s) 207, 218, 292, 417, 766
Sau96I GGNCC 2 cut(s) 591, 592
ScrFI CCNGG 1 cut(s) 732
SduI GDGCHC 2 cut(s) 595, 683
SfaNI GCATC 1 cut(s) 635
Sfr303I CCGCGG 1 cut(s) 769
SgrAI CRCCGGYG 1 cut(s) 383
SgrBI CCGCGG 1 cut(s) 769
SgrDI CGTCGACG 1 cut(s) 170
Sse9I AATT 6 cut(s) 6, 68, 113, 226, 420, 722
SsiI CCGC 9 cut(s) 86, 207, 218, 234, 291, 387, 469, 766, 768
SspMI CTAG 1 cut(s) 806
StyD4I CCNGG 1 cut(s) 730
StyI CCWWGG 1 cut(s) 306
TaiI ACGT 3 cut(s) 413, 544, 713
TaqI TCGA 5 cut(s) 98, 171, 189, 327, 349
TasI AATT 6 cut(s) 6, 68, 113, 226, 420, 722
TauI GCSGC 4 cut(s) 209, 220, 294, 768
TfiI GAWTC 5 cut(s) 191, 351, 429, 546, 600
TscAI CASTG 2 cut(s) 478, 688
TseFI GTSAC 1 cut(s) 638
TseI GCWGC 1 cut(s) 416
Tsp45I GTSAC 1 cut(s) 638
TspGWI ACGGA 3 cut(s) 189, 243, 552
TspRI CASTG 2 cut(s) 478, 688
XapI RAATTY 3 cut(s) 68, 113, 722
XmiI GTMKAC 1 cut(s) 171
XspI CTAG 1 cut(s) 806
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.