pycom08g06420

Protein of unknown function (DUF3054)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0011949
Physical Location & Seq
Reverse (-)
4994114 .. 4996029
1916 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g06420.4

Sequence Viewer

Length: 789 bp
ATGCTAGTGTTCGGCCAAGCTCCCGGCGGAGCCTTATCAGCCCGGCGGTGTTCTCTCACTTCCCCGCCAAAATTCTCAAACCCAAACCCTCCCCTCTTCTCTTCCCGCAGACCCAGCAGATTCTTCTCCAATGTAAACCCCTCAAAATCCCTCAATATCACGCTCGTAAAGGCCAACGGAGGCGTCGACTCGGCTTCCTCCACTGCCAAACAGGGCTTCTCCGCCTCATCACCTCCTCCTCCTCCTCCGCCTTCCGTCGATAACCAACCAGTTTTCGTGGGTCAGGAGAACATACCTCTAGAAGGCGTGATTCAGTTCGAGAAGCCCGATTCTTCTGCTCCATTGCTTGCTGGTGGGGATGTTTTGGCATTGCTTACGTTTGCTGCAATTGGAAGATTCAGCCATGGATTCTCCGTTTTCGATTTCGAAACACTGCGCACGGCTGACCCTTTTATTGCTGGGTGGTTTTTGAGTGCTTATTTCCTCGGAGGCTTTGGTGAGGATGGACGTGGAGTCAATGGACGATCGAAGGCTCTTACCGCTACGGCTAAGTCATGGGCCTTGGGAATTCCACTTGGGATAATCATAAGGGCATCAACAGCTGGCCACATTCCGCCAGTCAAATTCATGCTTGTAACAATGGGGAGCACTGCTGTTTTACTAATTGGATGGAGATCACTATTGTATAAGTTTCTTCCCGGTGATAAGAGCAAGAAGAGTGATGTGTATCGCCGCGGTAACCCATTTGAATTGTTTGAGTTGCTCACTTCATTAATACGAAGGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3054

Protein Analysis

263

Amino Acids

28.03

Weight (kDa)

10.16

Isoelectric Point (pI)

50.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011949)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44920
fragaria_vesca FvH4_2g32650
malus_domestica MD08G1077300.v1.1 MD15G1064800.v1.1
prunus_persica Prupe.1G418700_v2.0.a1
pyrus_communis pycom08g06420 pycom15g06150
rosa_chinensis RchiOBHm_Chr6g0310081
rosa_laevigata RLG00000010513
rosa_multiflora Rmu_sc0000294.1_g000021 Rmu_sc0038452.1_g000001
rosa_roxburghii Rroxscaffold_7G00158900
rosa_rugosa Rorug06G0377200 Rorug06G0377200
rosa_samantha Rh6AG491100 Rh6BG500500 Rh6CG505400 Rh6DG491400
rosa_wichuraiana Rw6G042730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 437
AccI GTMKAC 1 cut(s) 186
AccII CGCG 1 cut(s) 735
AcoI YGGCCR 2 cut(s) 13, 604
AcsI RAATTY 3 cut(s) 71, 567, 623
AcyI GRCGYC 1 cut(s) 183
AfiI CCNNNNNNNGG 1 cut(s) 302
AgsI TTSAA 1 cut(s) 749
AhdI GACNNNNNGTC 1 cut(s) 512
AjiI CACGTC 1 cut(s) 509
AjuI GAANNNNNNNTTGG 2 cut(s) 200, 232
AluBI AGCT 2 cut(s) 20, 602
AluI AGCT 2 cut(s) 20, 602
Alw21I GWGCWC 1 cut(s) 650
AoxI GGCC 4 cut(s) 13, 171, 558, 604
ApeKI GCWGC 1 cut(s) 383
ApoI RAATTY 3 cut(s) 71, 567, 623
AseI ATTAAT 1 cut(s) 773
AspLEI GCGC 1 cut(s) 438
AspS9I GGNCC 1 cut(s) 558
AsuC2I CCSGG 3 cut(s) 24, 43, 699
AsuHPI GGTGA 3 cut(s) 222, 509, 713
AsuII TTCGAA 1 cut(s) 426
BalI TGGCCA 1 cut(s) 606
Bbv12I GWGCWC 1 cut(s) 650
BbvI GCAGC 1 cut(s) 370
BccI CCATC 2 cut(s) 497, 663
BceAI ACGGC 2 cut(s) 456, 561
BcgI CGANNNNNNTGC 2 cut(s) 317, 351
BcnI CCSGG 3 cut(s) 24, 43, 699
BfaI CTAG 2 cut(s) 5, 299
BisI GCNGC 2 cut(s) 384, 733
BlsI GCNGC 2 cut(s) 385, 734
Bme1390I CCNGG 3 cut(s) 24, 43, 699
BmeRI GACNNNNNGTC 1 cut(s) 512
BmgBI CACGTC 1 cut(s) 509
BmgT120I GGNCC 1 cut(s) 558
BmiI GGNNCC 1 cut(s) 31
BmrFI CCNGG 3 cut(s) 24, 43, 699
BmsI GCATC 1 cut(s) 602
Bpu14I TTCGAA 1 cut(s) 426
BpuMI CCSGG 3 cut(s) 24, 43, 699
BsaBI GATNNNNATC 2 cut(s) 673, 726
BsaHI GRCGYC 1 cut(s) 183
BsaJI CCNNGG 4 cut(s) 403, 484, 561, 733
Bsc4I CCNNNNNNNGG 1 cut(s) 302
Bse1I ACTGG 2 cut(s) 269, 617
Bse3DI GCAATG 2 cut(s) 341, 368
Bse8I GATNNNNATC 2 cut(s) 673, 726
BseDI CCNNGG 4 cut(s) 403, 484, 561, 733
BseGI GGATG 3 cut(s) 364, 508, 674
BseJI GATNNNNATC 2 cut(s) 673, 726
BseLI CCNNNNNNNGG 1 cut(s) 302
BseMI GCAATG 2 cut(s) 341, 368
BseNI ACTGG 2 cut(s) 269, 617
BseRI GAGGAG 4 cut(s) 225, 228, 231, 234
BseXI GCAGC 1 cut(s) 370
BseYI CCCAGC 2 cut(s) 113, 458
Bsh1236I CGCG 1 cut(s) 735
Bsh1285I CGRYCG 1 cut(s) 527
BshFI GGCC 4 cut(s) 15, 173, 560, 606
BsiEI CGRYCG 1 cut(s) 527
BsiHKAI GWGCWC 1 cut(s) 650
BsiSI CCGG 3 cut(s) 24, 43, 699
BslI CCNNNNNNNGG 1 cut(s) 302
BsnI GGCC 4 cut(s) 15, 173, 560, 606
Bsp119I TTCGAA 1 cut(s) 426
Bsp1286I GDGCHC 1 cut(s) 650
Bsp143I GATC 2 cut(s) 524, 674
Bsp19I CCATGG 1 cut(s) 403
BspANI GGCC 4 cut(s) 15, 173, 560, 606
BspFNI CGCG 1 cut(s) 735
BspLI GGNNCC 1 cut(s) 31
BspT104I TTCGAA 1 cut(s) 426
BsrDI GCAATG 2 cut(s) 341, 368
BsrI ACTGG 2 cut(s) 269, 617
BssECI CCNNGG 4 cut(s) 403, 484, 561, 733
BssMI GATC 2 cut(s) 524, 674
BssNI GRCGYC 1 cut(s) 183
BssT1I CCWWGG 2 cut(s) 403, 561
Bst6I CTCTTC 3 cut(s) 101, 106, 710
BstACI GRCGYC 1 cut(s) 183
BstBI TTCGAA 1 cut(s) 426
BstC8I GCNNGC 2 cut(s) 348, 604
BstDEI CTNAG 1 cut(s) 549
BstDSI CCRYGG 2 cut(s) 403, 733
BstEII GGTNACC 1 cut(s) 737
BstENI CCTNNNNNAGG 1 cut(s) 300
BstF5I GGATG 3 cut(s) 364, 508, 674
BstFNI CGCG 1 cut(s) 735
BstHHI GCGC 1 cut(s) 438
BstKTI GATC 2 cut(s) 527, 677
BstMBI GATC 2 cut(s) 524, 674
BstMCI CGRYCG 1 cut(s) 527
BstMWI GCNNNNNNNGC 4 cut(s) 38, 114, 539, 599
BstPI GGTNACC 1 cut(s) 737
BstSCI CCNGG 3 cut(s) 22, 41, 697
BstUI CGCG 1 cut(s) 735
BstV1I GCAGC 1 cut(s) 370
BsuRI GGCC 4 cut(s) 15, 173, 560, 606
BtgI CCRYGG 2 cut(s) 403, 733
BtrI CACGTC 1 cut(s) 509
BtsCI GGATG 3 cut(s) 364, 508, 674
BtsI GCAGTG 3 cut(s) 201, 431, 648
BtsIMutI CAGTG 3 cut(s) 201, 431, 648
Cac8I GCNNGC 2 cut(s) 348, 604
CfoI GCGC 1 cut(s) 438
Cfr13I GGNCC 1 cut(s) 558
Cfr42I CCGCGG 1 cut(s) 736
CseI GACGC 1 cut(s) 172
CviAII CATG 3 cut(s) 404, 555, 628
DdeI CTNAG 1 cut(s) 549
DpnI GATC 2 cut(s) 526, 676
DpnII GATC 2 cut(s) 524, 674
DriI GACNNNNNGTC 1 cut(s) 512
EaeI YGGCCR 2 cut(s) 13, 604
Eam1104I CTCTTC 3 cut(s) 101, 106, 710
Eam1105I GACNNNNNGTC 1 cut(s) 512
EarI CTCTTC 3 cut(s) 101, 106, 710
EciI GGCGGA 4 cut(s) 42, 211, 237, 603
Eco130I CCWWGG 2 cut(s) 403, 561
Eco91I GGTNACC 1 cut(s) 737
EcoNI CCTNNNNNAGG 1 cut(s) 300
EcoO65I GGTNACC 1 cut(s) 737
EcoRI GAATTC 1 cut(s) 567
EcoT14I CCWWGG 2 cut(s) 403, 561
ErhI CCWWGG 2 cut(s) 403, 561
FaeI CATG 3 cut(s) 407, 558, 631
FaiI YATR 6 cut(s) 293, 405, 556, 587, 629, 687
FatI CATG 3 cut(s) 403, 554, 627
FauI CCCGC 2 cut(s) 72, 113
FblI GTMKAC 1 cut(s) 186
Fnu4HI GCNGC 2 cut(s) 384, 733
FokI GGATG 3 cut(s) 371, 515, 681
Fsp4HI GCNGC 2 cut(s) 384, 733
FspBI CTAG 2 cut(s) 5, 299
FspI TGCGCA 1 cut(s) 437
GlaI GCGC 1 cut(s) 437
GluI GCNGC 2 cut(s) 384, 733
GsaI CCCAGC 2 cut(s) 117, 462
HaeIII GGCC 4 cut(s) 15, 173, 560, 606
HapII CCGG 3 cut(s) 24, 43, 699
HgaI GACGC 1 cut(s) 172
HhaI GCGC 1 cut(s) 438
Hin1I GRCGYC 1 cut(s) 183
Hin1II CATG 3 cut(s) 407, 558, 631
Hin6I GCGC 1 cut(s) 436
HinP1I GCGC 1 cut(s) 436
HincII GTYRAC 1 cut(s) 187
HindII GTYRAC 1 cut(s) 187
HinfI GANTC 7 cut(s) 120, 188, 310, 329, 396, 408, 513
HpaII CCGG 3 cut(s) 24, 43, 699
HphI GGTGA 3 cut(s) 222, 509, 713
Hpy166II GTNNAC 2 cut(s) 136, 187
Hpy188I TCNGA 1 cut(s) 488
Hpy188III TCNNGA 3 cut(s) 284, 299, 319
Hpy8I GTNNAC 2 cut(s) 136, 187
Hpy99I CGWCG 2 cut(s) 188, 260
HpyAV CCTTC 4 cut(s) 261, 296, 523, 774
HpyCH4IV ACGT 2 cut(s) 377, 508
HpyCH4V TGCA 1 cut(s) 386
HpyF10VI GCNNNNNNNGC 4 cut(s) 38, 114, 539, 599
HpyF3I CTNAG 1 cut(s) 549
HpySE526I ACGT 2 cut(s) 377, 508
Hsp92I GRCGYC 1 cut(s) 183
Hsp92II CATG 3 cut(s) 407, 558, 631
HspAI GCGC 1 cut(s) 436
KspI CCGCGG 1 cut(s) 736
Kzo9I GATC 2 cut(s) 524, 674
LmnI GCTCC 4 cut(s) 25, 29, 343, 645
Lsp1109I GCAGC 1 cut(s) 370
LweI GCATC 1 cut(s) 602
MaeI CTAG 2 cut(s) 5, 299
MaeII ACGT 2 cut(s) 377, 508
MaeIII GTNAC 2 cut(s) 634, 737
MalI GATC 2 cut(s) 526, 676
MboI GATC 2 cut(s) 524, 674
MboII GAAGA 7 cut(s) 88, 93, 115, 324, 405, 686, 727
MfeI CAATTG 1 cut(s) 387
MhlI GDGCHC 1 cut(s) 650
MlsI TGGCCA 1 cut(s) 606
MluCI AATT 6 cut(s) 71, 387, 567, 623, 663, 749
MluNI TGGCCA 1 cut(s) 606
MlyI GAGTC 2 cut(s) 182, 522
Mox20I TGGCCA 1 cut(s) 606
MscI TGGCCA 1 cut(s) 606
MseI TTAA 1 cut(s) 773
Msp20I TGGCCA 1 cut(s) 606
MspA1I CMGCKG 2 cut(s) 602, 735
MspI CCGG 3 cut(s) 24, 43, 699
MspR9I CCNGG 3 cut(s) 24, 43, 699
MunI CAATTG 1 cut(s) 387
MvnI CGCG 1 cut(s) 735
MwoI GCNNNNNNNGC 4 cut(s) 38, 114, 539, 599
NciI CCSGG 3 cut(s) 24, 43, 699
NcoI CCATGG 1 cut(s) 403
NdeII GATC 2 cut(s) 524, 674
NlaIII CATG 3 cut(s) 407, 558, 631
NlaIV GGNNCC 1 cut(s) 31
NmeAIII GCCGAG 1 cut(s) 170
NsbI TGCGCA 1 cut(s) 437
NspV TTCGAA 1 cut(s) 426
PcsI WCGNNNNNNNCGW 2 cut(s) 183, 324
PfeI GAWTC 5 cut(s) 120, 310, 329, 396, 408
PkrI GCNGC 2 cut(s) 385, 734
Ple19I CGATCG 1 cut(s) 527
PleI GAGTC 2 cut(s) 182, 521
PpsI GAGTC 2 cut(s) 182, 521
PshBI ATTAAT 1 cut(s) 773
PspEI GGTNACC 1 cut(s) 737
PspFI CCCAGC 2 cut(s) 113, 458
PspN4I GGNNCC 1 cut(s) 31
PspPI GGNCC 1 cut(s) 558
PvuI CGATCG 1 cut(s) 527
PvuII CAGCTG 1 cut(s) 602
SacII CCGCGG 1 cut(s) 736
SalI GTCGAC 1 cut(s) 185
SaqAI TTAA 1 cut(s) 773
SatI GCNGC 2 cut(s) 384, 733
Sau3AI GATC 2 cut(s) 524, 674
Sau96I GGNCC 1 cut(s) 558
SchI GAGTC 2 cut(s) 182, 522
ScrFI CCNGG 3 cut(s) 24, 43, 699
SduI GDGCHC 1 cut(s) 650
SetI ASST 7 cut(s) 22, 235, 298, 380, 511, 604, 785
SfaNI GCATC 1 cut(s) 602
Sfr303I CCGCGG 1 cut(s) 736
SfuI TTCGAA 1 cut(s) 426
SgrBI CCGCGG 1 cut(s) 736
Sse9I AATT 6 cut(s) 71, 387, 567, 623, 663, 749
SspMI CTAG 2 cut(s) 5, 299
StyD4I CCNGG 3 cut(s) 22, 41, 697
StyI CCWWGG 2 cut(s) 403, 561
TaiI ACGT 2 cut(s) 380, 511
TaqI TCGA 6 cut(s) 186, 258, 318, 420, 426, 527
TasI AATT 6 cut(s) 71, 387, 567, 623, 663, 749
TauI GCSGC 1 cut(s) 735
TfiI GAWTC 5 cut(s) 120, 310, 329, 396, 408
Tru1I TTAA 1 cut(s) 773
Tru9I TTAA 1 cut(s) 773
TscAI CASTG 3 cut(s) 208, 438, 655
TseI GCWGC 1 cut(s) 383
TspDTI ATGAA 2 cut(s) 616, 759
TspGWI ACGGA 3 cut(s) 192, 244, 403
TspRI CASTG 3 cut(s) 208, 438, 655
VspI ATTAAT 1 cut(s) 773
XagI CCTNNNNNAGG 1 cut(s) 300
XapI RAATTY 3 cut(s) 71, 567, 623
XbaI TCTAGA 1 cut(s) 298
XmiI GTMKAC 1 cut(s) 186
XspI CTAG 2 cut(s) 5, 299
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.