MD08G1078600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0013622
Physical Location & Seq
Reverse (-)
6549966 .. 6551891
1926 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1078600.v1.1.491

Sequence Viewer

Length: 1164 bp
ATGGCTTCCACCCCCAAGCCGGTAAAACTTGCAGAGCTCCTCCAAGAGCAGCAAGAGCCCTTTGTCCTAGAGGTTTACCTATTTGAAAGGGGTTACATGAGAAAAACCTCGAGTACAAATAGCAGTTCGAGGATTACAAGTAGAACTTCTAGCCAGAAACTCATCAGATCATCCAGTTGGGGTAATCTCAGCTGCCATAAAGTATTGAGATCTGTGTGTAACAAGCTTGTTTCAAGACATAGTGAGACAACAAGCACTAAGGGTTGTGAAAACCGAGAGGGGAAGCCCAATGGTGGCGCCGAGAAGCGGAGAAGTCGCCGAGAGAGAGGGGAAACGGATACATTTTCATCTGCCAGCAGTAGGACACAGTATGATTCGTGCTCCGAGGGCGAAAAAGAATCAAAAGAAGAAGCTCCCGCTTCGTTGCGAAATAAGCATGATGCTTCCCCACTTGCTGCAGACGCTACTCAAATTTCGGAATCCTGTAATATGAAAGAGAGAAAGATAGAACGGCTCAACGGACAGTCATCGAAGATTCACCGTAAGCAACAAAACCCCTTTTCAGTACCAGAAGATGAACCTCCCCATGCAAACGCAAGTTCACCTCTTTATAACAACAGGTATAAACTGCACACCATGTCTAGAGAGAAGGAAACTTCATGTCCATCCACTTATAGCTTCAACAAGATTACAGAAGACCCAGATTTATCATCTTCTCTCTGGGAACTACTTTTCCACCCGCCATTAGAGAAGCCGAGAGCCTCTGGAATTTCGGAAAAGCTCGAGCCTGTCCGGTCTAGCATCAATCCATCCCCTCATTTCGCAAAATCGAAAAGGGTGTTGCAGCAGAAGAGGCAGCTCCTGTTCGATTGCGTGAGGGAGATGACGGAAAATCACGCAAAGAAATCGAAAGAGCAGCAGCGGCATAACCCCAACGGATTTCTTGGAGCCGAAGAGATTGGGAAGCTGATTTATGAGAAGTTGGGGAGTTGGGGAGGTAAACATGCTGGGCACGAAGCCAACGTCAATGTTTTGTTGGAGTTGGACTTGTTGGGCCCCCGTCAAGAATGGGATGACAGTTACCAAGAGAAAAGAGAAATTGGGTCCGAAATTGGAGACGCCATTTTAGAAGAGATCATCAAAGGCATTGTCTCTGAAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4378

Protein Analysis

388

Amino Acids

43.73

Weight (kDa)

8.53

Isoelectric Point (pI)

67.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4378 PF14309 268 - 380 4.1e-06 Domain of unknown function (DUF4378)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013622)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g32690
malus_domestica MD08G1078600.v1.1 MD15G1065400.v1.1
prunus_persica Prupe.1G419500_v2.0.a1
pyrus_communis pycom08g06500 pycom15g06190
rosa_chinensis RchiOBHm_Chr6g0309981
rosa_laevigata RLG00000010519
rosa_multiflora Rmu_sc0000294.1_g000010
rosa_roxburghii Rroxscaffold_7G00158980
rosa_rugosa Rorug06G0376500 Rorug06G0376600
rosa_samantha Rh6AG490300 Rh6BG499800 Rh6CG504600 Rh6CG504700 Rh6DG490700
rosa_wichuraiana Rw6G042680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 612
AccB1I GGYRCC 1 cut(s) 296
AciI CCGC 4 cut(s) 307, 417, 740, 922
AcsI RAATTY 2 cut(s) 471, 768
AcyI GRCGYC 2 cut(s) 297, 1119
AfaI GTAC 2 cut(s) 115, 567
AfiI CCNNNNNNNGG 4 cut(s) 19, 293, 306, 360
AgsI TTSAA 3 cut(s) 86, 234, 682
AloI GAACNNNNNNTCC 2 cut(s) 717, 749
AluBI AGCT 8 cut(s) 37, 192, 226, 413, 678, 781, 859, 967
AluI AGCT 8 cut(s) 37, 192, 226, 413, 678, 781, 859, 967
Alw21I GWGCWC 2 cut(s) 39, 383
Alw26I GTCTC 3 cut(s) 239, 1110, 1156
AlwNI CAGNNNCTG 1 cut(s) 862
Ama87I CYCGRG 2 cut(s) 109, 782
AoxI GGCC 1 cut(s) 1054
ApaI GGGCCC 1 cut(s) 1058
ApeKI GCWGC 7 cut(s) 49, 192, 455, 844, 856, 916, 919
ApoI RAATTY 2 cut(s) 471, 768
ArsI GACNNNNNNTTYG 3 cut(s) 1008, 1040, 1134
AspLEI GCGC 1 cut(s) 299
AspS9I GGNCC 3 cut(s) 1054, 1055, 1104
AsuHPI GGTGA 2 cut(s) 530, 594
AvaI CYCGRG 2 cut(s) 109, 782
AvaII GGWCC 1 cut(s) 1104
BaeGI GKGCMC 2 cut(s) 1014, 1058
BanI GGYRCC 1 cut(s) 296
BanII GRGCYC 3 cut(s) 39, 60, 1058
BbsI GAAGAC 1 cut(s) 702
Bbv12I GWGCWC 2 cut(s) 39, 383
BbvI GCAGC 7 cut(s) 61, 179, 442, 856, 868, 928, 931
BccI CCATC 2 cut(s) 673, 817
BceAI ACGGC 1 cut(s) 527
BcgI CGANNNNNNTGC 2 cut(s) 888, 922
BciVI GTATCC 1 cut(s) 331
BcoDI GTCTC 3 cut(s) 239, 1110, 1156
BfaI CTAG 4 cut(s) 68, 150, 642, 798
BfmI CTRYAG 1 cut(s) 456
BfoI RGCGCY 1 cut(s) 300
BfuI GTATCC 1 cut(s) 331
BglII AGATCT 1 cut(s) 209
BisI GCNGC 8 cut(s) 50, 193, 456, 845, 857, 917, 920, 923
BlsI GCNGC 8 cut(s) 51, 194, 457, 846, 858, 918, 921, 924
Bme18I GGWCC 1 cut(s) 1104
BmeT110I CYCGRG 2 cut(s) 109, 782
BmgT120I GGNCC 3 cut(s) 1054, 1055, 1104
BmiI GGNNCC 5 cut(s) 298, 949, 1056, 1057, 1105
BmsI GCATC 2 cut(s) 430, 810
BpiI GAAGAC 1 cut(s) 702
BsaHI GRCGYC 2 cut(s) 297, 1119
BsaJI CCNNGG 1 cut(s) 384
BsaWI WCCGGW 1 cut(s) 792
Bsc4I CCNNNNNNNGG 4 cut(s) 19, 293, 306, 360
Bse118I RCCGGY 1 cut(s) 19
Bse1I ACTGG 1 cut(s) 174
BseDI CCNNGG 1 cut(s) 384
BseGI GGATG 4 cut(s) 170, 665, 809, 1078
BseLI CCNNNNNNNGG 4 cut(s) 19, 293, 306, 360
BseMII CTCAG 1 cut(s) 202
BseNI ACTGG 1 cut(s) 174
BseRI GAGGAG 1 cut(s) 29
BseSI GKGCMC 2 cut(s) 1014, 1058
BseXI GCAGC 7 cut(s) 61, 179, 442, 856, 868, 928, 931
BseYI CCCAGC 1 cut(s) 1007
BsgI GTGCAG 1 cut(s) 614
BshFI GGCC 1 cut(s) 1056
BshNI GGYRCC 1 cut(s) 296
BsiHKAI GWGCWC 2 cut(s) 39, 383
BsiHKCI CYCGRG 2 cut(s) 109, 782
BsiSI CCGG 2 cut(s) 20, 793
BslI CCNNNNNNNGG 4 cut(s) 19, 293, 306, 360
BsmAI GTCTC 3 cut(s) 239, 1110, 1156
BsmBI CGTCTC 1 cut(s) 1110
BsnI GGCC 1 cut(s) 1056
BsoBI CYCGRG 2 cut(s) 109, 782
Bsp120I GGGCCC 1 cut(s) 1054
Bsp1286I GDGCHC 5 cut(s) 39, 60, 383, 1014, 1058
Bsp143I GATC 3 cut(s) 167, 209, 1134
BspACI CCGC 4 cut(s) 307, 417, 740, 922
BspANI GGCC 1 cut(s) 1056
BspCNI CTCAG 1 cut(s) 201
BspLI GGNNCC 5 cut(s) 298, 949, 1056, 1057, 1105
BspMAI CTGCAG 1 cut(s) 460
BspT107I GGYRCC 1 cut(s) 296
BsrFI RCCGGY 1 cut(s) 19
BsrI ACTGG 1 cut(s) 174
BssAI RCCGGY 1 cut(s) 19
BssECI CCNNGG 1 cut(s) 384
BssMI GATC 3 cut(s) 167, 209, 1134
BssNI GRCGYC 2 cut(s) 297, 1119
Bst4CI ACNGT 4 cut(s) 369, 525, 542, 1079
Bst6I CTCTTC 4 cut(s) 845, 948, 1125, 1152
BstACI GRCGYC 2 cut(s) 297, 1119
BstC8I GCNNGC 1 cut(s) 355
BstDEI CTNAG 2 cut(s) 188, 258
BstF5I GGATG 4 cut(s) 170, 665, 809, 1078
BstH2I RGCGCY 1 cut(s) 300
BstHHI GCGC 1 cut(s) 299
BstKTI GATC 3 cut(s) 170, 212, 1137
BstMAI GTCTC 3 cut(s) 239, 1110, 1156
BstMBI GATC 3 cut(s) 167, 209, 1134
BstMWI GCNNNNNNNGC 6 cut(s) 55, 387, 433, 461, 853, 922
BstNSI RCATGY 1 cut(s) 1007
BstSFI CTRYAG 1 cut(s) 456
BstSLI GKGCMC 2 cut(s) 1014, 1058
BstV1I GCAGC 7 cut(s) 61, 179, 442, 856, 868, 928, 931
BstV2I GAAGAC 1 cut(s) 702
BstX2I RGATCY 1 cut(s) 209
BstYI RGATCY 1 cut(s) 209
BsuI GTATCC 1 cut(s) 331
BsuRI GGCC 1 cut(s) 1056
BtsCI GGATG 4 cut(s) 170, 665, 809, 1078
Cac8I GCNNGC 1 cut(s) 355
CaiI CAGNNNCTG 1 cut(s) 862
CfoI GCGC 1 cut(s) 299
Cfr10I RCCGGY 1 cut(s) 19
Cfr13I GGNCC 3 cut(s) 1054, 1055, 1104
CseI GACGC 2 cut(s) 470, 1127
Csp6I GTAC 2 cut(s) 114, 566
CviAII CATG 6 cut(s) 97, 437, 587, 637, 660, 1004
CviQI GTAC 2 cut(s) 114, 566
DdeI CTNAG 2 cut(s) 188, 258
DinI GGCGCC 1 cut(s) 298
DpnI GATC 3 cut(s) 169, 211, 1136
DpnII GATC 3 cut(s) 167, 209, 1134
Eam1104I CTCTTC 4 cut(s) 845, 948, 1125, 1152
EarI CTCTTC 4 cut(s) 845, 948, 1125, 1152
Ecl136II GAGCTC 1 cut(s) 37
Eco24I GRGCYC 3 cut(s) 39, 60, 1058
Eco47I GGWCC 1 cut(s) 1104
Eco53kI GAGCTC 1 cut(s) 37
Eco88I CYCGRG 2 cut(s) 109, 782
EcoICRI GAGCTC 1 cut(s) 37
EcoO109I RGGNCCY 1 cut(s) 1055
EcoT38I GRGCYC 3 cut(s) 39, 60, 1058
EgeI GGCGCC 1 cut(s) 298
EheI GGCGCC 1 cut(s) 298
Esp3I CGTCTC 1 cut(s) 1110
FaeI CATG 6 cut(s) 100, 440, 590, 640, 663, 1007
FalI AAGNNNNNCTT 2 cut(s) 130, 162
FatI CATG 6 cut(s) 96, 436, 586, 636, 659, 1003
FauI CCCGC 2 cut(s) 424, 747
Fnu4HI GCNGC 8 cut(s) 50, 193, 456, 845, 857, 917, 920, 923
FokI GGATG 4 cut(s) 157, 652, 796, 1085
FriOI GRGCYC 3 cut(s) 39, 60, 1058
Fsp4HI GCNGC 8 cut(s) 50, 193, 456, 845, 857, 917, 920, 923
FspBI CTAG 4 cut(s) 68, 150, 642, 798
GlaI GCGC 1 cut(s) 298
GluI GCNGC 8 cut(s) 50, 193, 456, 845, 857, 917, 920, 923
GsaI CCCAGC 1 cut(s) 1011
HaeII RGCGCY 1 cut(s) 300
HaeIII GGCC 1 cut(s) 1056
HapII CCGG 2 cut(s) 20, 793
HgaI GACGC 2 cut(s) 470, 1127
HhaI GCGC 1 cut(s) 299
Hin1I GRCGYC 2 cut(s) 297, 1119
Hin1II CATG 6 cut(s) 100, 440, 590, 640, 663, 1007
Hin6I GCGC 1 cut(s) 297
HinP1I GCGC 1 cut(s) 297
HindIII AAGCTT 1 cut(s) 224
HinfI GANTC 4 cut(s) 374, 398, 479, 535
HpaII CCGG 2 cut(s) 20, 793
HphI GGTGA 2 cut(s) 530, 594
Hpy166II GTNNAC 3 cut(s) 76, 602, 1001
Hpy188I TCNGA 6 cut(s) 167, 385, 478, 775, 1108, 1156
Hpy188III TCNNGA 4 cut(s) 234, 642, 765, 1064
Hpy8I GTNNAC 3 cut(s) 76, 602, 1001
HpyAV CCTTC 1 cut(s) 643
HpyCH4III ACNGT 4 cut(s) 369, 525, 542, 1079
HpyCH4IV ACGT 1 cut(s) 1023
HpyCH4V TGCA 5 cut(s) 32, 458, 590, 631, 844
HpyF10VI GCNNNNNNNGC 6 cut(s) 55, 387, 433, 461, 853, 922
HpyF3I CTNAG 2 cut(s) 188, 258
HpySE526I ACGT 1 cut(s) 1023
Hsp92I GRCGYC 2 cut(s) 297, 1119
Hsp92II CATG 6 cut(s) 100, 440, 590, 640, 663, 1007
HspAI GCGC 1 cut(s) 297
KasI GGCGCC 1 cut(s) 296
Kzo9I GATC 3 cut(s) 167, 209, 1134
LmnI GCTCC 5 cut(s) 42, 386, 418, 864, 947
Lsp1109I GCAGC 7 cut(s) 61, 179, 442, 856, 868, 928, 931
LweI GCATC 2 cut(s) 430, 810
MaeI CTAG 4 cut(s) 68, 150, 642, 798
MaeII ACGT 1 cut(s) 1023
MaeIII GTNAC 3 cut(s) 92, 218, 1079
MalI GATC 3 cut(s) 169, 211, 1136
MboI GATC 3 cut(s) 167, 209, 1134
MboII GAAGA 8 cut(s) 419, 544, 584, 705, 707, 862, 965, 1142
MflI RGATCY 1 cut(s) 209
MhlI GDGCHC 5 cut(s) 39, 60, 383, 1014, 1058
MluCI AATT 4 cut(s) 471, 768, 1098, 1110
Mly113I GGCGCC 1 cut(s) 297
MmeI TCCRAC 2 cut(s) 1017, 1023
MspA1I CMGCKG 2 cut(s) 192, 922
MspI CCGG 2 cut(s) 20, 793
MwoI GCNNNNNNNGC 6 cut(s) 55, 387, 433, 461, 853, 922
NarI GGCGCC 1 cut(s) 297
NdeII GATC 3 cut(s) 167, 209, 1134
NlaIII CATG 6 cut(s) 100, 440, 590, 640, 663, 1007
NlaIV GGNNCC 5 cut(s) 298, 949, 1056, 1057, 1105
NmeAIII GCCGAG 3 cut(s) 325, 344, 780
NspI RCATGY 1 cut(s) 1007
PaeR7I CTCGAG 2 cut(s) 109, 782
PcsI WCGNNNNNNNCGW 1 cut(s) 1020
PfeI GAWTC 4 cut(s) 374, 398, 479, 535
PkrI GCNGC 8 cut(s) 51, 194, 457, 846, 858, 918, 921, 924
PluTI GGCGCC 1 cut(s) 300
PsiI TTATAA 1 cut(s) 612
Psp124BI GAGCTC 1 cut(s) 39
PspFI CCCAGC 1 cut(s) 1007
PspN4I GGNNCC 5 cut(s) 298, 949, 1056, 1057, 1105
PspOMI GGGCCC 1 cut(s) 1054
PspPI GGNCC 3 cut(s) 1054, 1055, 1104
PspXI VCTCGAGB 2 cut(s) 109, 782
PstI CTGCAG 1 cut(s) 460
PstNI CAGNNNCTG 1 cut(s) 862
PsuI RGATCY 1 cut(s) 209
PvuII CAGCTG 1 cut(s) 192
RsaI GTAC 2 cut(s) 115, 567
RsaNI GTAC 2 cut(s) 114, 566
SacI GAGCTC 1 cut(s) 39
SatI GCNGC 8 cut(s) 50, 193, 456, 845, 857, 917, 920, 923
Sau3AI GATC 3 cut(s) 167, 209, 1134
Sau96I GGNCC 3 cut(s) 1054, 1055, 1104
SduI GDGCHC 5 cut(s) 39, 60, 383, 1014, 1058
SfaNI GCATC 2 cut(s) 430, 810
SfcI CTRYAG 1 cut(s) 456
SfoI GGCGCC 1 cut(s) 298
Sfr274I CTCGAG 2 cut(s) 109, 782
SinI GGWCC 1 cut(s) 1104
SlaI CTCGAG 2 cut(s) 109, 782
SmlI CTYRAG 2 cut(s) 109, 782
SmoI CTYRAG 2 cut(s) 109, 782
Sse9I AATT 4 cut(s) 471, 768, 1098, 1110
SsiI CCGC 4 cut(s) 307, 417, 740, 922
SspDI GGCGCC 1 cut(s) 296
SspMI CTAG 4 cut(s) 68, 150, 642, 798
SstI GAGCTC 1 cut(s) 39
TaaI ACNGT 4 cut(s) 369, 525, 542, 1079
TaiI ACGT 1 cut(s) 1026
TaqI TCGA 7 cut(s) 110, 128, 530, 783, 830, 867, 908
TasI AATT 4 cut(s) 471, 768, 1098, 1110
TatI WGTACW 1 cut(s) 113
TauI GCSGC 1 cut(s) 925
TfiI GAWTC 4 cut(s) 374, 398, 479, 535
TseI GCWGC 7 cut(s) 49, 192, 455, 844, 856, 916, 919
TspDTI ATGAA 4 cut(s) 336, 506, 591, 648
TspGWI ACGGA 4 cut(s) 350, 534, 902, 951
VpaK11BI GGWCC 1 cut(s) 1104
XapI RAATTY 2 cut(s) 471, 768
XbaI TCTAGA 1 cut(s) 641
XceI RCATGY 1 cut(s) 1007
XhoI CTCGAG 2 cut(s) 109, 782
XspI CTAG 4 cut(s) 68, 150, 642, 798
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.