MD15G1065400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0013622
Physical Location & Seq
Reverse (-)
4557637 .. 4559191
1555 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1065400.v1.1.491

Sequence Viewer

Length: 648 bp
ATGAAAGAGAGAAAGTTACAGATAGAACGGCTCAACGGACAGTCATCGGAGATTCACCGTAAGCAACAAAACCCCGATTCGGTACCAGAAGATAAACCTCCCCATGCAAATGCAAGTTTACCTCTTCATAACAAGTATGAACTGCAAACTCCATGTCCATCCACTTATAGCTTCAACAAGATTACAGAAGACTCAATATTATCATCTTCTCTCTGGGAACTACTTTTCCACCCACCATTTGAGAACCCAACAGCTTCTGGAGTTTCAGAAATGCTAGAGCCTGCCCGGTCTAGCATCAATCCATCCCCTCATTTCGCAAAATCGAAGAGGGTGTGGCAGCAGACAAGGCTGCTCCTGTTTGATTGCGTGAGGGAGATGATGGAAAATCATGCGAAGAAATTGAAAGAGCAGCAGCAGCATAACTGCGACCGATTTCTTGGAGCCGAAGAGATTGGGAAGCTGGTTTATGAGAAGTTGGGGAGTTGGGGGGATAAACAGGCTGGGCACCAAGGCAACGTCAACTTTTTGTTGGAGTGGGACTTGTTGGCTTCGGCTGAAGAATGGGGTGATAATTACCAAGAGAGAAGGGAAATTGGGTCGGAAATTGGAGACGCCATTTTACAAGACATCATCAAAGACATATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4378

Protein Analysis

216

Amino Acids

24.74

Weight (kDa)

5.52

Isoelectric Point (pI)

59.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4378 PF14309 85 - 212 5.9e-09 Domain of unknown function (DUF4378)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013622)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g32690
malus_domestica MD08G1078600.v1.1 MD15G1065400.v1.1
prunus_persica Prupe.1G419500_v2.0.a1
pyrus_communis pycom08g06500 pycom15g06190
rosa_chinensis RchiOBHm_Chr6g0309981
rosa_laevigata RLG00000010519
rosa_multiflora Rmu_sc0000294.1_g000010
rosa_roxburghii Rroxscaffold_7G00158980
rosa_rugosa Rorug06G0376500 Rorug06G0376600
rosa_samantha Rh6AG490300 Rh6BG499800 Rh6CG504600 Rh6CG504700 Rh6DG490700
rosa_wichuraiana Rw6G042680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 82
AccB1I GGYRCC 2 cut(s) 82, 504
AcuI CTGAAG 1 cut(s) 576
AcyI GRCGYC 1 cut(s) 612
AfaI GTAC 1 cut(s) 84
AfiI CCNNNNNNNGG 1 cut(s) 79
AgsI TTSAA 2 cut(s) 175, 403
AloI GAACNNNNNNTCC 2 cut(s) 210, 242
AluBI AGCT 3 cut(s) 171, 254, 460
AluI AGCT 3 cut(s) 171, 254, 460
Alw26I GTCTC 1 cut(s) 603
AlwNI CAGNNNCTG 1 cut(s) 257
ApeKI GCWGC 5 cut(s) 337, 349, 409, 412, 415
ArsI GACNNNNNNTTYG 2 cut(s) 139, 171
Asp718I GGTACC 1 cut(s) 82
AsuC2I CCSGG 1 cut(s) 286
AsuHPI GGTGA 2 cut(s) 47, 578
BaeGI GKGCMC 1 cut(s) 507
BanI GGYRCC 2 cut(s) 82, 504
BbsI GAAGAC 1 cut(s) 195
BbvI GCAGC 5 cut(s) 336, 349, 421, 424, 427
BccI CCATC 3 cut(s) 166, 310, 373
BceAI ACGGC 1 cut(s) 44
BcnI CCSGG 1 cut(s) 286
BcoDI GTCTC 1 cut(s) 603
BfaI CTAG 2 cut(s) 275, 291
BisI GCNGC 5 cut(s) 338, 350, 410, 413, 416
BlsI GCNGC 5 cut(s) 339, 351, 411, 414, 417
Bme1390I CCNGG 1 cut(s) 286
BmiI GGNNCC 3 cut(s) 84, 442, 506
BmrFI CCNGG 1 cut(s) 286
BmsI GCATC 1 cut(s) 303
BpiI GAAGAC 1 cut(s) 195
BpmI CTGGAG 1 cut(s) 279
BpuMI CCSGG 1 cut(s) 286
BsaHI GRCGYC 1 cut(s) 612
BsaJI CCNNGG 1 cut(s) 508
Bsc4I CCNNNNNNNGG 1 cut(s) 79
BseDI CCNNGG 1 cut(s) 508
BseGI GGATG 2 cut(s) 158, 302
BseLI CCNNNNNNNGG 1 cut(s) 79
BseSI GKGCMC 1 cut(s) 507
BseXI GCAGC 5 cut(s) 336, 349, 421, 424, 427
BseYI CCCAGC 1 cut(s) 500
Bsh1285I CGRYCG 1 cut(s) 430
BshNI GGYRCC 2 cut(s) 82, 504
BsiEI CGRYCG 1 cut(s) 430
BsiSI CCGG 1 cut(s) 286
BslFI GGGAC 1 cut(s) 551
BslI CCNNNNNNNGG 1 cut(s) 79
BsmAI GTCTC 1 cut(s) 603
BsmBI CGTCTC 1 cut(s) 603
BsmFI GGGAC 1 cut(s) 551
Bsp1286I GDGCHC 1 cut(s) 507
BspLI GGNNCC 3 cut(s) 84, 442, 506
BspT107I GGYRCC 2 cut(s) 82, 504
BssECI CCNNGG 1 cut(s) 508
BssNI GRCGYC 1 cut(s) 612
BssT1I CCWWGG 1 cut(s) 508
Bst4CI ACNGT 2 cut(s) 42, 59
Bst6I CTCTTC 3 cut(s) 129, 320, 441
BstACI GRCGYC 1 cut(s) 612
BstC8I GCNNGC 1 cut(s) 282
BstF5I GGATG 2 cut(s) 158, 302
BstMAI GTCTC 1 cut(s) 603
BstMCI CGRYCG 1 cut(s) 430
BstMWI GCNNNNNNNGC 2 cut(s) 346, 415
BstSCI CCNGG 1 cut(s) 284
BstSLI GKGCMC 1 cut(s) 507
BstV1I GCAGC 5 cut(s) 336, 349, 421, 424, 427
BstV2I GAAGAC 1 cut(s) 195
BtsCI GGATG 2 cut(s) 158, 302
Cac8I GCNNGC 1 cut(s) 282
CaiI CAGNNNCTG 1 cut(s) 257
CseI GACGC 1 cut(s) 620
Csp6I GTAC 1 cut(s) 83
CviAII CATG 3 cut(s) 104, 153, 389
CviQI GTAC 1 cut(s) 83
Eam1104I CTCTTC 3 cut(s) 129, 320, 441
EarI CTCTTC 3 cut(s) 129, 320, 441
Eco130I CCWWGG 1 cut(s) 508
Eco57I CTGAAG 1 cut(s) 576
EcoT14I CCWWGG 1 cut(s) 508
ErhI CCWWGG 1 cut(s) 508
Esp3I CGTCTC 1 cut(s) 603
FaeI CATG 3 cut(s) 107, 156, 392
FaiI YATR 9 cut(s) 105, 129, 138, 154, 168, 390, 420, 468, 641
FaqI GGGAC 1 cut(s) 551
FatI CATG 3 cut(s) 103, 152, 388
Fnu4HI GCNGC 5 cut(s) 338, 350, 410, 413, 416
FokI GGATG 2 cut(s) 145, 289
Fsp4HI GCNGC 5 cut(s) 338, 350, 410, 413, 416
FspBI CTAG 2 cut(s) 275, 291
GluI GCNGC 5 cut(s) 338, 350, 410, 413, 416
GsaI CCCAGC 1 cut(s) 504
GsuI CTGGAG 1 cut(s) 279
HapII CCGG 1 cut(s) 286
HgaI GACGC 1 cut(s) 620
Hin1I GRCGYC 1 cut(s) 612
Hin1II CATG 3 cut(s) 107, 156, 392
HincII GTYRAC 1 cut(s) 520
HindII GTYRAC 1 cut(s) 520
HinfI GANTC 3 cut(s) 52, 77, 191
HpaII CCGG 1 cut(s) 286
HphI GGTGA 2 cut(s) 47, 578
Hpy166II GTNNAC 2 cut(s) 119, 520
Hpy188I TCNGA 4 cut(s) 49, 268, 601, 647
Hpy188III TCNNGA 1 cut(s) 258
Hpy8I GTNNAC 2 cut(s) 119, 520
HpyAV CCTTC 1 cut(s) 579
HpyCH4III ACNGT 2 cut(s) 42, 59
HpyCH4IV ACGT 1 cut(s) 516
HpyCH4V TGCA 3 cut(s) 107, 113, 145
HpyF10VI GCNNNNNNNGC 2 cut(s) 346, 415
HpySE526I ACGT 1 cut(s) 516
Hsp92I GRCGYC 1 cut(s) 612
Hsp92II CATG 3 cut(s) 107, 156, 392
KpnI GGTACC 1 cut(s) 86
LmnI GCTCC 2 cut(s) 357, 440
LpnPI CCDG 9 cut(s) 99, 199, 243, 294, 299, 368, 446, 482, 486
Lsp1109I GCAGC 5 cut(s) 336, 349, 421, 424, 427
LweI GCATC 1 cut(s) 303
MaeI CTAG 2 cut(s) 275, 291
MaeII ACGT 1 cut(s) 516
MaeIII GTNAC 1 cut(s) 15
MboII GAAGA 8 cut(s) 101, 116, 198, 200, 337, 406, 458, 569
MhlI GDGCHC 1 cut(s) 507
MluCI AATT 4 cut(s) 398, 571, 591, 603
MlyI GAGTC 1 cut(s) 185
MmeI TCCRAC 2 cut(s) 510, 579
MnlI CCTC 5 cut(s) 108, 132, 318, 321, 363
MslI CAYNNNNRTG 1 cut(s) 108
MspI CCGG 1 cut(s) 286
MspR9I CCNGG 1 cut(s) 286
MwoI GCNNNNNNNGC 2 cut(s) 346, 415
NciI CCSGG 1 cut(s) 286
NlaIII CATG 3 cut(s) 107, 156, 392
NlaIV GGNNCC 3 cut(s) 84, 442, 506
PfeI GAWTC 2 cut(s) 52, 77
PkrI GCNGC 5 cut(s) 339, 351, 411, 414, 417
PleI GAGTC 1 cut(s) 185
PpsI GAGTC 1 cut(s) 185
PspFI CCCAGC 1 cut(s) 500
PspN4I GGNNCC 3 cut(s) 84, 442, 506
PstNI CAGNNNCTG 1 cut(s) 257
RsaI GTAC 1 cut(s) 84
RsaNI GTAC 1 cut(s) 83
RseI CAYNNNNRTG 1 cut(s) 108
SatI GCNGC 5 cut(s) 338, 350, 410, 413, 416
SchI GAGTC 1 cut(s) 185
ScrFI CCNGG 1 cut(s) 286
SduI GDGCHC 1 cut(s) 507
SetI ASST 6 cut(s) 100, 124, 173, 256, 462, 519
SfaNI GCATC 1 cut(s) 303
SmiMI CAYNNNNRTG 1 cut(s) 108
Sse9I AATT 4 cut(s) 398, 571, 591, 603
SspI AATATT 1 cut(s) 198
SspMI CTAG 2 cut(s) 275, 291
StyD4I CCNGG 1 cut(s) 284
StyI CCWWGG 1 cut(s) 508
TaaI ACNGT 2 cut(s) 42, 59
TaiI ACGT 1 cut(s) 519
TaqI TCGA 1 cut(s) 323
TaqII GACCGA 1 cut(s) 444
TasI AATT 4 cut(s) 398, 571, 591, 603
TfiI GAWTC 2 cut(s) 52, 77
TseI GCWGC 5 cut(s) 337, 349, 409, 412, 415
TspDTI ATGAA 3 cut(s) 17, 116, 153
TspGWI ACGGA 1 cut(s) 51
XspI CTAG 2 cut(s) 275, 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.