Rh6AG490300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0013622
Physical Location & Seq
Forward (+)
66684997 .. 66686330
1334 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG490300.1

Sequence Viewer

Length: 1098 bp
ATGGCTTCCACAACAACAAAACCAGTTAAACAACTTGGAGAGCTTCTCCAAGAGCAGCAAGAACCCTTTGTCCTAGATGTTTACCTATTTGAAAGAGGGTGTCTGAGAAAGAGCTTGAGTTCAAGCAATAGCATTAATTCAAGCAAGAGTTTAAAGAGGTCATCTAGTTGTGGACCAATCAAGAGCAAAAAGGGTACTCCTAGCTTCTCCAAGATACTGAGATCTGTCTATAACAAGCTTGTTTCCAAAAATGGTGGCTCGAGAACTAAGAGTTCCGGAGAAGAAGAGGGAAAGTTTGATGCCAACACTGAGATTACTCTTCCTAGTGATTGCCTTGGAGATTTGGATGAATTTTCTTCTTCTAGTAGCAGAACACGGTATGAATCATGCTGTGATAGTGACAAAGATGAAGCTTGCGCCACTGTTTTGTTGCAGAATGAAGATGAAGCCTCGCTTGCAGTAGAAGGCACTTCTCAAGTTCCAAATCTCTGCAATATGAAAGAAGAGACAAAGATAGCACTGCTCAATGGGGAATCATCGAAGAAGAAGAAGAAGAAGACGCAGACTAAGCAACACACCTCAATTTCAGTACTAGAAGATAGACCTTCTCATGCAGTACCCAACTCCGTGTTATCAGCTTCTCTTTGGGAACTACTTTTCCAGCCACCATTGGAGAGGCCAAGAGATACTTTTGGAGTTCCGGAAATGCTAGAGCCGGTAATCAGATCGAATTCTTCTCCACACAAATCGAAAAGGATGTTGCAGCAGACAAGGCAGCTTCTTTTCGATTGTGTGAGAGAGGTGACAGAATCCCATGCAAAGCAAATGAAAGAGGAGCAGAACAGCGCAAAGTTTTTGGGGGCAGAAGAACTGGGAAAGCTGATTTCTGAGAAATTAAGGATTTGGGATAGACAGGCTGGGGATGAAACCAACATCGACTTTTTGTTGGATTCAGATATCCTGAGTTCAGCTGAAGAATGGAATTGTAATGAACAAGAAGAGAGAGAGATTTGTTGCGAAATTGGGGACGCCATTTTAGAAGAGATCATCAAAGAAACTGTTGCTCTACCGATTAGTTTAGATCCTGTTTCCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4378

Protein Analysis

365

Amino Acids

40.67

Weight (kDa)

5.18

Isoelectric Point (pI)

62.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4378 PF14309 248 - 352 9.8e-08 Domain of unknown function (DUF4378)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013622)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g32690
malus_domestica MD08G1078600.v1.1 MD15G1065400.v1.1
prunus_persica Prupe.1G419500_v2.0.a1
pyrus_communis pycom08g06500 pycom15g06190
rosa_chinensis RchiOBHm_Chr6g0309981
rosa_laevigata RLG00000010519
rosa_multiflora Rmu_sc0000294.1_g000010
rosa_roxburghii Rroxscaffold_7G00158980
rosa_rugosa Rorug06G0376500 Rorug06G0376600
rosa_samantha Rh6AG490300 Rh6BG499800 Rh6CG504600 Rh6CG504700 Rh6DG490700
rosa_wichuraiana Rw6G042680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 2 cut(s) 275, 700
AclWI GGATC 1 cut(s) 1076
AcsI RAATTY 2 cut(s) 350, 730
AcuI CTGAAG 1 cut(s) 993
AcyI GRCGYC 1 cut(s) 1029
AfaI GTAC 3 cut(s) 196, 591, 618
AgsI TTSAA 3 cut(s) 92, 123, 141
AloI GAACNNNNNNTCC 4 cut(s) 54, 86, 642, 674
AluBI AGCT 9 cut(s) 43, 114, 204, 238, 413, 638, 778, 880, 971
AluI AGCT 9 cut(s) 43, 114, 204, 238, 413, 638, 778, 880, 971
Alw26I GTCTC 1 cut(s) 500
AlwI GGATC 1 cut(s) 1076
Ama87I CYCGRG 1 cut(s) 259
Aor13HI TCCGGA 2 cut(s) 275, 700
AoxI GGCC 1 cut(s) 677
ApeKI GCWGC 3 cut(s) 55, 763, 775
ApoI RAATTY 2 cut(s) 350, 730
AseI ATTAAT 1 cut(s) 135
AspLEI GCGC 2 cut(s) 419, 848
AspS9I GGNCC 1 cut(s) 173
AsuHPI GGTGA 1 cut(s) 814
AvaI CYCGRG 1 cut(s) 259
AvaII GGWCC 1 cut(s) 173
BbsI GAAGAC 1 cut(s) 563
BbvI GCAGC 3 cut(s) 67, 775, 787
BcoDI GTCTC 1 cut(s) 500
BfaI CTAG 7 cut(s) 74, 165, 201, 324, 363, 593, 710
BglII AGATCT 1 cut(s) 221
BisI GCNGC 3 cut(s) 56, 764, 776
BlsI GCNGC 3 cut(s) 57, 765, 777
BmcAI AGTACT 1 cut(s) 591
Bme18I GGWCC 1 cut(s) 173
BmeT110I CYCGRG 1 cut(s) 259
BmgT120I GGNCC 1 cut(s) 173
BmrI ACTGGG 1 cut(s) 881
BmsI GCATC 1 cut(s) 289
BmuI ACTGGG 1 cut(s) 881
BpiI GAAGAC 1 cut(s) 563
BplI GAGNNNNNCTC 2 cut(s) 30, 62
BpuEI CTTGAG 2 cut(s) 136, 459
BsaHI GRCGYC 1 cut(s) 1029
BsaJI CCNNGG 1 cut(s) 334
BsaWI WCCGGW 2 cut(s) 275, 700
Bse118I RCCGGY 1 cut(s) 715
Bse1I ACTGG 2 cut(s) 23, 876
BseAI TCCGGA 2 cut(s) 275, 700
BseDI CCNNGG 1 cut(s) 334
BseGI GGATG 3 cut(s) 352, 762, 928
BseMII CTCAG 5 cut(s) 95, 209, 300, 879, 953
BseNI ACTGG 2 cut(s) 23, 876
BseRI GAGGAG 1 cut(s) 848
BseXI GCAGC 3 cut(s) 67, 775, 787
BseYI CCCAGC 1 cut(s) 917
BshFI GGCC 1 cut(s) 679
BsiHKCI CYCGRG 1 cut(s) 259
BsiSI CCGG 3 cut(s) 276, 701, 716
BslFI GGGAC 1 cut(s) 1040
BsmAI GTCTC 1 cut(s) 500
BsmFI GGGAC 1 cut(s) 1040
BsnI GGCC 1 cut(s) 679
BsoBI CYCGRG 1 cut(s) 259
Bsp13I TCCGGA 2 cut(s) 275, 700
Bsp143I GATC 4 cut(s) 221, 725, 1044, 1081
BspANI GGCC 1 cut(s) 679
BspCNI CTCAG 5 cut(s) 96, 210, 301, 880, 954
BspEI TCCGGA 2 cut(s) 275, 700
BspPI GGATC 1 cut(s) 1076
BsrFI RCCGGY 1 cut(s) 715
BsrI ACTGG 2 cut(s) 23, 876
BssAI RCCGGY 1 cut(s) 715
BssECI CCNNGG 1 cut(s) 334
BssMI GATC 4 cut(s) 221, 725, 1044, 1081
BssNI GRCGYC 1 cut(s) 1029
BssT1I CCWWGG 1 cut(s) 334
Bst4CI ACNGT 3 cut(s) 378, 424, 1060
Bst6I CTCTTC 5 cut(s) 279, 324, 498, 993, 1035
BstACI GRCGYC 1 cut(s) 1029
BstC8I GCNNGC 2 cut(s) 415, 456
BstDEI CTNAG 7 cut(s) 104, 218, 267, 309, 567, 888, 962
BstF5I GGATG 3 cut(s) 352, 762, 928
BstHHI GCGC 2 cut(s) 419, 848
BstKTI GATC 4 cut(s) 224, 728, 1047, 1084
BstMAI GTCTC 1 cut(s) 500
BstMBI GATC 4 cut(s) 221, 725, 1044, 1081
BstMWI GCNNNNNNNGC 3 cut(s) 455, 568, 772
BstV1I GCAGC 3 cut(s) 67, 775, 787
BstV2I GAAGAC 1 cut(s) 563
BstX2I RGATCY 2 cut(s) 221, 1081
BstYI RGATCY 2 cut(s) 221, 1081
BsuRI GGCC 1 cut(s) 679
BtsCI GGATG 3 cut(s) 352, 762, 928
BtsI GCAGTG 1 cut(s) 518
BtsIMutI CAGTG 3 cut(s) 306, 420, 518
Cac8I GCNNGC 2 cut(s) 415, 456
CfoI GCGC 2 cut(s) 419, 848
Cfr10I RCCGGY 1 cut(s) 715
Cfr13I GGNCC 1 cut(s) 173
CseI GACGC 2 cut(s) 568, 1037
Csp6I GTAC 3 cut(s) 195, 590, 617
CspCI CAANNNNNGTGG 2 cut(s) 235, 270
CviAII CATG 3 cut(s) 387, 611, 815
CviQI GTAC 3 cut(s) 195, 590, 617
DdeI CTNAG 7 cut(s) 104, 218, 267, 309, 567, 888, 962
DpnI GATC 4 cut(s) 223, 727, 1046, 1083
DpnII GATC 4 cut(s) 221, 725, 1044, 1081
DraI TTTAAA 1 cut(s) 153
Eam1104I CTCTTC 5 cut(s) 279, 324, 498, 993, 1035
EarI CTCTTC 5 cut(s) 279, 324, 498, 993, 1035
Eco130I CCWWGG 1 cut(s) 334
Eco32I GATATC 1 cut(s) 958
Eco47I GGWCC 1 cut(s) 173
Eco57I CTGAAG 1 cut(s) 993
Eco88I CYCGRG 1 cut(s) 259
EcoRI GAATTC 1 cut(s) 730
EcoRV GATATC 1 cut(s) 958
EcoT14I CCWWGG 1 cut(s) 334
ErhI CCWWGG 1 cut(s) 334
FaeI CATG 3 cut(s) 390, 614, 818
FaiI YATR 6 cut(s) 231, 381, 388, 497, 612, 816
FalI AAGNNNNNCTT 4 cut(s) 438, 470, 673, 705
FaqI GGGAC 1 cut(s) 1040
FatI CATG 3 cut(s) 386, 610, 814
Fnu4HI GCNGC 3 cut(s) 56, 764, 776
FokI GGATG 3 cut(s) 359, 769, 935
Fsp4HI GCNGC 3 cut(s) 56, 764, 776
FspBI CTAG 7 cut(s) 74, 165, 201, 324, 363, 593, 710
GlaI GCGC 2 cut(s) 418, 847
GluI GCNGC 3 cut(s) 56, 764, 776
GsaI CCCAGC 1 cut(s) 921
HaeIII GGCC 1 cut(s) 679
HapII CCGG 3 cut(s) 276, 701, 716
HgaI GACGC 2 cut(s) 568, 1037
HhaI GCGC 2 cut(s) 419, 848
Hin1I GRCGYC 1 cut(s) 1029
Hin1II CATG 3 cut(s) 390, 614, 818
Hin6I GCGC 2 cut(s) 417, 846
HinP1I GCGC 2 cut(s) 417, 846
HindIII AAGCTT 2 cut(s) 236, 411
HinfI GANTC 4 cut(s) 383, 533, 809, 950
HpaII CCGG 3 cut(s) 276, 701, 716
HphI GGTGA 1 cut(s) 814
Hpy166II GTNNAC 2 cut(s) 82, 173
Hpy188I TCNGA 5 cut(s) 105, 725, 889, 955, 1097
Hpy188III TCNNGA 5 cut(s) 181, 261, 276, 701, 961
Hpy8I GTNNAC 2 cut(s) 82, 173
HpyAV CCTTC 2 cut(s) 458, 615
HpyCH4III ACNGT 3 cut(s) 378, 424, 1060
HpyCH4V TGCA 6 cut(s) 433, 458, 492, 614, 763, 818
HpyF10VI GCNNNNNNNGC 3 cut(s) 455, 568, 772
HpyF3I CTNAG 7 cut(s) 104, 218, 267, 309, 567, 888, 962
Hsp92I GRCGYC 1 cut(s) 1029
Hsp92II CATG 3 cut(s) 390, 614, 818
HspAI GCGC 2 cut(s) 417, 846
Kpn2I TCCGGA 2 cut(s) 275, 700
Kzo9I GATC 4 cut(s) 221, 725, 1044, 1081
LmnI GCTCC 1 cut(s) 835
LpnPI CCDG 9 cut(s) 36, 289, 674, 714, 729, 857, 899, 903, 974
Lsp1109I GCAGC 3 cut(s) 67, 775, 787
LweI GCATC 1 cut(s) 289
MaeI CTAG 7 cut(s) 74, 165, 201, 324, 363, 593, 710
MaeIII GTNAC 2 cut(s) 398, 802
MalI GATC 4 cut(s) 223, 727, 1046, 1083
MboI GATC 4 cut(s) 221, 725, 1044, 1081
MflI RGATCY 2 cut(s) 221, 1081
MluCI AATT 7 cut(s) 136, 350, 582, 730, 893, 982, 1020
MmeI TCCRAC 1 cut(s) 927
MnlI CCTC 8 cut(s) 89, 150, 280, 460, 589, 669, 793, 826
MroI TCCGGA 2 cut(s) 275, 700
MseI TTAA 4 cut(s) 27, 135, 152, 896
MspA1I CMGCKG 1 cut(s) 971
MspI CCGG 3 cut(s) 276, 701, 716
MwoI GCNNNNNNNGC 3 cut(s) 455, 568, 772
NdeII GATC 4 cut(s) 221, 725, 1044, 1081
NlaIII CATG 3 cut(s) 390, 614, 818
NmuCI GTSAC 2 cut(s) 398, 802
PaeR7I CTCGAG 1 cut(s) 259
PfeI GAWTC 4 cut(s) 383, 533, 809, 950
PkrI GCNGC 3 cut(s) 57, 765, 777
PshBI ATTAAT 1 cut(s) 135
PspFI CCCAGC 1 cut(s) 917
PspPI GGNCC 1 cut(s) 173
PsuI RGATCY 2 cut(s) 221, 1081
PvuII CAGCTG 1 cut(s) 971
RsaI GTAC 3 cut(s) 196, 591, 618
RsaNI GTAC 3 cut(s) 195, 590, 617
SaqAI TTAA 4 cut(s) 27, 135, 152, 896
SatI GCNGC 3 cut(s) 56, 764, 776
Sau3AI GATC 4 cut(s) 221, 725, 1044, 1081
Sau96I GGNCC 1 cut(s) 173
ScaI AGTACT 1 cut(s) 591
SfaNI GCATC 1 cut(s) 289
Sfr274I CTCGAG 1 cut(s) 259
SinI GGWCC 1 cut(s) 173
SlaI CTCGAG 1 cut(s) 259
SmlI CTYRAG 3 cut(s) 115, 259, 474
SmoI CTYRAG 3 cut(s) 115, 259, 474
Sse9I AATT 7 cut(s) 136, 350, 582, 730, 893, 982, 1020
SspMI CTAG 7 cut(s) 74, 165, 201, 324, 363, 593, 710
StyI CCWWGG 1 cut(s) 334
TaaI ACNGT 3 cut(s) 378, 424, 1060
TaqI TCGA 6 cut(s) 260, 539, 728, 749, 786, 936
TasI AATT 7 cut(s) 136, 350, 582, 730, 893, 982, 1020
TatI WGTACW 1 cut(s) 589
TfiI GAWTC 4 cut(s) 383, 533, 809, 950
Tru1I TTAA 4 cut(s) 27, 135, 152, 896
Tru9I TTAA 4 cut(s) 27, 135, 152, 896
TscAI CASTG 3 cut(s) 313, 427, 525
TseFI GTSAC 2 cut(s) 398, 802
TseI GCWGC 3 cut(s) 55, 763, 775
Tsp45I GTSAC 2 cut(s) 398, 802
TspDTI ATGAA 9 cut(s) 363, 396, 423, 453, 459, 512, 842, 939, 1005
TspGWI ACGGA 1 cut(s) 616
TspRI CASTG 3 cut(s) 313, 427, 525
VpaK11BI GGWCC 1 cut(s) 173
VspI ATTAAT 1 cut(s) 135
XapI RAATTY 2 cut(s) 350, 730
XhoI CTCGAG 1 cut(s) 259
XspI CTAG 7 cut(s) 74, 165, 201, 324, 363, 593, 710
ZrmI AGTACT 1 cut(s) 591
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.